LOCUS       NM_017755               3056 bp    mRNA    linear   PRI 17-JUN-2024
DEFINITION  Homo sapiens NOP2/Sun RNA methyltransferase 2 (NSUN2), transcript
            variant 1, mRNA.
ACCESSION   NM_017755
VERSION     NM_017755.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3056)
  AUTHORS   Zhang,X., An,K., Ge,X., Sun,Y., Wei,J., Ren,W., Wang,H., Wang,Y.,
            Du,Y., He,L., Li,O., Zhou,S., Shi,Y., Ren,T., Yang,Y.G., Kan,Q. and
            Tian,X.
  TITLE     NSUN2/YBX1 promotes the progression of breast cancer by enhancing
            HGH1 mRNA stability through m5C methylation
  JOURNAL   Breast Cancer Res 26 (1), 94 (2024)
   PUBMED   38844963
  REMARK    GeneRIF: NSUN2/YBX1 promotes the progression of breast cancer by
            enhancing HGH1 mRNA stability through m[5]C methylation.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3056)
  AUTHORS   Wang,R., Xue,W., Kan,F., Zhang,H., Wang,D., Wang,L. and Wang,J.
  TITLE     NSUN2 affects diabetic retinopathy progression by regulating MUC1
            expression through RNA m5C methylation
  JOURNAL   J Transl Med 22 (1), 476 (2024)
   PUBMED   38764010
  REMARK    GeneRIF: NSUN2 affects diabetic retinopathy progression by
            regulating MUC1 expression through RNA m[5]C methylation.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 3056)
  AUTHORS   Du,X., Cheng,C., Yang,Y., Fan,B., Wang,P., Xia,H., Ni,X., Liu,Q.,
            Lu,L. and Wei,L.
  TITLE     NSUN2 promotes lung adenocarcinoma progression through stabilizing
            PIK3R2 mRNA in an m5C-dependent manner
  JOURNAL   Mol Carcinog 63 (5), 962-976 (2024)
   PUBMED   38411298
  REMARK    GeneRIF: NSUN2 promotes lung adenocarcinoma progression through
            stabilizing PIK3R2 mRNA in an m[5]C-dependent manner.
REFERENCE   4  (bases 1 to 3056)
  AUTHORS   Wang,X., Liu,D., Hua,K. and Fang,L.
  TITLE     LncRNA HOST2 promotes NSUN2-mediated breast cancer progression via
            interaction with ELAVL1
  JOURNAL   Cell Signal 117, 111112 (2024)
   PUBMED   38387687
  REMARK    GeneRIF: LncRNA HOST2 promotes NSUN2-mediated breast cancer
            progression via interaction with ELAVL1.
REFERENCE   5  (bases 1 to 3056)
  AUTHORS   Chen,Y., Jiang,Z., Zhang,C., Zhang,L., Chen,H., Xiao,N., Bai,L.,
            Liu,H. and Wan,J.
  TITLE     5-Methylcytosine transferase NSUN2 drives NRF2-mediated ferroptosis
            resistance in non-small cell lung cancer
  JOURNAL   J Biol Chem 300 (4), 106793 (2024)
   PUBMED   38403250
  REMARK    GeneRIF: 5-Methylcytosine transferase NSUN2 drives NRF2-mediated
            ferroptosis resistance in non-small cell lung cancer.
REFERENCE   6  (bases 1 to 3056)
  AUTHORS   Sakita-Suto,S., Kanda,A., Suzuki,F., Sato,S., Takata,T. and
            Tatsuka,M.
  TITLE     Aurora-B regulates RNA methyltransferase NSUN2
  JOURNAL   Mol Biol Cell 18 (3), 1107-1117 (2007)
   PUBMED   17215513
  REMARK    GeneRIF: These results indicate that Aurora-B participates to
            regulate the assembly of nucleolar RNA-processing machinery and the
            RNA methyltransferase activity of NSUN2 via phosphorylation at
            Ser139 during mitosis.
REFERENCE   7  (bases 1 to 3056)
  AUTHORS   Najmabadi,H., Motazacker,M.M., Garshasbi,M., Kahrizi,K.,
            Tzschach,A., Chen,W., Behjati,F., Hadavi,V., Nieh,S.E.,
            Abedini,S.S., Vazifehmand,R., Firouzabadi,S.G., Jamali,P.,
            Falah,M., Seifati,S.M., Gruters,A., Lenzner,S., Jensen,L.R.,
            Ruschendorf,F., Kuss,A.W. and Ropers,H.H.
  TITLE     Homozygosity mapping in consanguineous families reveals extreme
            heterogeneity of non-syndromic autosomal recessive mental
            retardation and identifies 8 novel gene loci
  JOURNAL   Hum Genet 121 (1), 43-48 (2007)
   PUBMED   17120046
REFERENCE   8  (bases 1 to 3056)
  AUTHORS   Brzezicha,B., Schmidt,M., Makalowska,I., Jarmolowski,A.,
            Pienkowska,J. and Szweykowska-Kulinska,Z.
  TITLE     Identification of human tRNA:m5C methyltransferase catalysing
            intron-dependent m5C formation in the first position of the
            anticodon of the pre-tRNA Leu (CAA)
  JOURNAL   Nucleic Acids Res 34 (20), 6034-6043 (2006)
   PUBMED   17071714
  REMARK    GeneRIF: First report showing intron-dependent methylation of human
            pre-tRNA Leu(CAA) and identification of human gene encoding tRNA
            methylase(Trm4) responsible for this reaction.
REFERENCE   9  (bases 1 to 3056)
  AUTHORS   Ballif,B.A., Villen,J., Beausoleil,S.A., Schwartz,D. and Gygi,S.P.
  TITLE     Phosphoproteomic analysis of the developing mouse brain
  JOURNAL   Mol Cell Proteomics 3 (11), 1093-1101 (2004)
   PUBMED   15345747
REFERENCE   10 (bases 1 to 3056)
  AUTHORS   Beausoleil,S.A., Jedrychowski,M., Schwartz,D., Elias,J.E.,
            Villen,J., Li,J., Cohn,M.A., Cantley,L.C. and Gygi,S.P.
  TITLE     Large-scale characterization of HeLa cell nuclear phosphoproteins
  JOURNAL   Proc Natl Acad Sci U S A 101 (33), 12130-12135 (2004)
   PUBMED   15302935
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BF000644.1, AB255451.1 and
            BC001041.2.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_017755.5.
            
            Summary: This gene encodes a methyltransferase that catalyzes the
            methylation of cytosine to 5-methylcytosine (m5C) at position 34 of
            intron-containing tRNA(Leu)(CAA) precursors. This modification is
            necessary to stabilize the anticodon-codon pairing and correctly
            translate the mRNA. Alternatively spliced transcript variants
            encoding different isoforms have been noted for this gene.[provided
            by RefSeq, Mar 2011].
            
            Transcript Variant: This variant (1) represents the predominant
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC137083.1, AK291144.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000264670.11/ ENSP00000264670.6
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-14                BF000644.1         3-16                c
            15-1878             AB255451.1         330-2193
            1879-3056           BC001041.2         1551-2728
FEATURES             Location/Qualifiers
     source          1..3056
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5p15.31"
     gene            1..3056
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /note="NOP2/Sun RNA methyltransferase 2"
                     /db_xref="GeneID:54888"
                     /db_xref="HGNC:HGNC:25994"
                     /db_xref="MIM:610916"
     exon            1..161
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /inference="alignment:Splign:2.1.0"
     CDS             66..2369
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /EC_number="2.1.1.203"
                     /note="isoform 1 is encoded by transcript variant 1;
                     5-methycytoisine methyltransferase; Myc-induced
                     SUN-domain-containing protein; NOL1/NOP2/Sun domain
                     family, member 2; tRNA methyltransferase 4 homolog; tRNA
                     (cytosine-5-)-methyltransferase NSUN2; tRNA
                     (cytosine(34)-C(5))-methyltransferase; NOP2/Sun RNA
                     methyltransferase family member 2; substrate of
                     AIM1/Aurora kinase B; myc-induced SUN domain-containing
                     protein; mRNA cytosine C(5)-methyltransferase; tRNA
                     cytosine C(5)-methyltransferase; RNA cytosine
                     C(5)-methyltransferase NSUN2"
                     /codon_start=1
                     /product="RNA cytosine C(5)-methyltransferase NSUN2
                     isoform 1"
                     /protein_id="NP_060225.4"
                     /db_xref="CCDS:CCDS3869.1"
                     /db_xref="GeneID:54888"
                     /db_xref="HGNC:HGNC:25994"
                     /db_xref="MIM:610916"
                     /translation="MGRRSRGRRLQQQQRPEDAEDGAEGGGKRGEAGWEGGYPEIVKE
                     NKLFEHYYQELKIVPEGEWGQFMDALREPLPATLRITGYKSHAKEILHCLKNKYFKEL
                     EDLEVDGQKVEVPQPLSWYPEELAWHTNLSRKILRKSPHLEKFHQFLVSETESGNISR
                     QEAVSMIPPLLLNVRPHHKILDMCAAPGSKTTQLIEMLHADMNVPFPEGFVIANDVDN
                     KRCYLLVHQAKRLSSPCIMVVNHDASSIPRLQIDVDGRKEILFYDRILCDVPCSGDGT
                     MRKNIDVWKKWTTLNSLQLHGLQLRIATRGAEQLAEGGRMVYSTCSLNPIEDEAVIAS
                     LLEKSEGALELADVSNELPGLKWMPGITQWKVMTKDGQWFTDWDAVPHSRHTQIRPTM
                     FPPKDPEKLQAMHLERCLRILPHHQNTGGFFVAVLVKKSSMPWNKRQPKLQGKSAETR
                     ESTQLSPADLTEGKPTDPSKLESPSFTGTGDTEIAHATEDLENNGSKKDGVCGPPPSK
                     KMKLFGFKEDPFVFIPEDDPLFPPIEKFYALDPSFPRMNLLTRTTEGKKRQLYMVSKE
                     LRNVLLNNSEKMKVINTGIKVWCRNNSGEEFDCAFRLAQEGIYTLYPFINSRIITVSM
                     EDVKILLTQENPFFRKLSSETYSQAKDLAKGSIVLKYEPDSANPDALQCPIVLCGWRG
                     KASIRTFVPKNERLHYLRMMGLEVLGEKKKEGVILTNESAASTGQPDNDVTEGQRAGE
                     PNSPDAEEANSPDVTAGCDPAGVHPPR"
     exon            162..319
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /inference="alignment:Splign:2.1.0"
     exon            320..424
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /inference="alignment:Splign:2.1.0"
     exon            425..530
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /inference="alignment:Splign:2.1.0"
     exon            531..602
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /inference="alignment:Splign:2.1.0"
     exon            603..687
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /inference="alignment:Splign:2.1.0"
     exon            688..880
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /inference="alignment:Splign:2.1.0"
     exon            881..955
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /inference="alignment:Splign:2.1.0"
     exon            956..1086
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /inference="alignment:Splign:2.1.0"
     exon            1087..1160
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /inference="alignment:Splign:2.1.0"
     exon            1161..1291
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /inference="alignment:Splign:2.1.0"
     exon            1292..1388
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /inference="alignment:Splign:2.1.0"
     exon            1389..1573
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /inference="alignment:Splign:2.1.0"
     exon            1574..1666
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /inference="alignment:Splign:2.1.0"
     exon            1667..1802
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /inference="alignment:Splign:2.1.0"
     exon            1803..1883
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /inference="alignment:Splign:2.1.0"
     exon            1884..2022
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /inference="alignment:Splign:2.1.0"
     exon            2023..2062
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /inference="alignment:Splign:2.1.0"
     exon            2063..3056
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3037..3042
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /note="hexamer: AATAAA"
     polyA_site      3056
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /note="major polyA site"
ORIGIN      
        1 gtagggctag agttctggcc gtggcgggcc ggtttctgcg tgctgcgtgc gcggccgcgt
       61 gggctatggg gcggcggtcg cggggtcggc ggctccagca acagcagcgg ccggaggacg
      121 cggaggatgg cgccgagggt ggtggaaagc gcggcgaggc gggctgggaa ggaggctacc
      181 ccgagatcgt caaggagaac aagctgttcg agcactacta ccaggagctc aagatcgtgc
      241 ccgagggcga gtggggccag ttcatggacg ctctcaggga gccgctcccg gccactttaa
      301 gaattactgg ttacaaaagc cacgcaaaag agattctcca ttgcttaaag aacaaatatt
      361 ttaaggaatt ggaggacctg gaggtggacg gtcagaaagt tgaagttcca cagccactga
      421 gttggtatcc tgaagaactt gcctggcaca caaatttaag tcgaaaaatc ttgagaaaat
      481 cgccacactt ggaaaagttt catcagtttc tagttagtga aacagaatct ggaaatatta
      541 gtcgtcaaga agctgttagc atgatcccac cactgctcct caacgtgcgg cctcatcata
      601 agatcttaga tatgtgtgca gcacctggct caaagaccac acagttaatt gaaatgctac
      661 atgccgacat gaatgtcccc tttccagagg gatttgttat tgcgaatgat gtggacaaca
      721 agcgctgcta cctgctcgtc catcaagcca agaggctgag cagcccctgc atcatggtgg
      781 tcaaccatga tgcctccagc atacccaggc tccagataga tgtggacggc aggaaagaga
      841 tcctcttcta tgatcgaatt ttatgtgatg tcccttgcag tggagacggc actatgagaa
      901 aaaacattga tgtttggaaa aagtggacca ccttaaatag cttgcagcta catggcttac
      961 agctgcggat tgcaacacgc ggggctgaac agctggctga aggtggaagg atggtgtatt
     1021 ccacgtgttc actaaaccct attgaggatg aagcagtcat agcatcttta ctggaaaaaa
     1081 gtgaaggtgc tttggagctt gctgatgtgt ctaatgaact gccagggctg aagtggatgc
     1141 ctggaatcac acagtggaag gtaatgacga aagatgggca gtggtttaca gactgggacg
     1201 ctgttcctca cagcagacac acccagatcc gacctaccat gttccctccg aaggacccag
     1261 aaaagctgca ggccatgcac ctggagcgat gccttaggat attaccccat catcagaata
     1321 ctggagggtt ttttgtggca gtattggtga aaaaatcttc aatgccgtgg aataaacgtc
     1381 agccaaagct tcagggtaaa tctgcagaga ccagagaaag cacacagctg agccctgcag
     1441 atctcacaga agggaaaccc acagatccct ctaagctgga aagtccgtca ttcacaggaa
     1501 ctggtgacac agaaatagct catgcaactg aggatttaga gaataatggc agtaagaaag
     1561 atggcgtgtg tggtcctcct ccatcaaaga aaatgaagtt atttggattt aaagaagatc
     1621 catttgtatt tattcctgaa gatgacccat tatttccacc tattgagaaa ttttatgctt
     1681 tggatccttc attcccaagg atgaatttgt taactcggac tacagaaggg aagaaaaggc
     1741 agctctacat ggtttctaag gagttgcgga atgtgctgct gaataacagt gagaagatga
     1801 aggttattaa cacggggatc aaagtctggt gtagaaataa cagcggtgaa gagtttgact
     1861 gtgctttccg gctggcacag gagggaatat atacattgta tccatttatt aactcaagaa
     1921 ttattactgt atcaatggaa gatgttaaga tactgttgac ccaggaaaat ccctttttta
     1981 gaaaactcag cagtgagacc tacagtcaag caaaggacct ggcaaaggga agcatcgtgc
     2041 tgaagtatga accagattct gcgaatccag acgctctgca gtgtcccatc gtcttatgcg
     2101 gatggcgggg aaaggcctcc attcgaactt ttgtgcccaa gaatgaacgg cttcattatc
     2161 tcaggatgat ggggctggag gtattgggag aaaagaagaa ggaaggggtt atcctcacaa
     2221 atgagagtgc agccagcacc ggacagccag acaatgacgt gactgaggga cagagagcag
     2281 gagagcccaa cagcccagat gcagaagagg ccaacagtcc agacgtgaca gcaggctgtg
     2341 acccggcggg ggtccatcca ccccggtgag caggcccaag gcagcggggg cccacacccc
     2401 tcacacgcaa aactggcttc ttctggtcac tggtgtctga aaccaaatcc agagcagcct
     2461 gtggcctgta aagcatatat ttctaatgac tgcagactgg tgggatcata ggagccttct
     2521 gaatgaccag gactgctttc tttggagctg atgaaaatgt actcttttag cgtgttagaa
     2581 atcacttgtt ttattttgtt tctttggcca agctgggtct agtgtttctt ttgctgggaa
     2641 tagactttca aaagttgtac ttctatcaag aaacaaaact gcccttgcag aaatttcagg
     2701 tcttttgtta agcctgtatt ggtcttaagg tgcagtattt tttaaattat tatttataga
     2761 aagaatctat aaattcttgg ggaagtgtgt tataagcttt aataattaca ttgagctgca
     2821 cctcagtggt gtgtcattaa catgcagtgg ggttaatatc tgaggcctca gatgactttg
     2881 tgccttttgg aataaagggt aaaataaact ctcccagagt aagagctgta tcgtgaattg
     2941 tcatactaat tattgagggg gacttatgtg cttttattga atggagtgct ttacaatttt
     3001 tatttttaaa tggggttggg atccttggaa tatttcaata aaattgataa aatata
//
