LOCUS       NM_001193455            2951 bp    mRNA    linear   PRI 17-JUN-2024
DEFINITION  Homo sapiens NOP2/Sun RNA methyltransferase 2 (NSUN2), transcript
            variant 2, mRNA.
ACCESSION   NM_001193455
VERSION     NM_001193455.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Zhang,X., An,K., Ge,X., Sun,Y., Wei,J., Ren,W., Wang,H., Wang,Y.,
            Du,Y., He,L., Li,O., Zhou,S., Shi,Y., Ren,T., Yang,Y.G., Kan,Q. and
            Tian,X.
  TITLE     NSUN2/YBX1 promotes the progression of breast cancer by enhancing
            HGH1 mRNA stability through m5C methylation
  JOURNAL   Breast Cancer Res 26 (1), 94 (2024)
   PUBMED   38844963
  REMARK    GeneRIF: NSUN2/YBX1 promotes the progression of breast cancer by
            enhancing HGH1 mRNA stability through m[5]C methylation.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Wang,R., Xue,W., Kan,F., Zhang,H., Wang,D., Wang,L. and Wang,J.
  TITLE     NSUN2 affects diabetic retinopathy progression by regulating MUC1
            expression through RNA m5C methylation
  JOURNAL   J Transl Med 22 (1), 476 (2024)
   PUBMED   38764010
  REMARK    GeneRIF: NSUN2 affects diabetic retinopathy progression by
            regulating MUC1 expression through RNA m[5]C methylation.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 2951)
  AUTHORS   Du,X., Cheng,C., Yang,Y., Fan,B., Wang,P., Xia,H., Ni,X., Liu,Q.,
            Lu,L. and Wei,L.
  TITLE     NSUN2 promotes lung adenocarcinoma progression through stabilizing
            PIK3R2 mRNA in an m5C-dependent manner
  JOURNAL   Mol Carcinog 63 (5), 962-976 (2024)
   PUBMED   38411298
  REMARK    GeneRIF: NSUN2 promotes lung adenocarcinoma progression through
            stabilizing PIK3R2 mRNA in an m[5]C-dependent manner.
REFERENCE   4  (bases 1 to 2951)
  AUTHORS   Wang,X., Liu,D., Hua,K. and Fang,L.
  TITLE     LncRNA HOST2 promotes NSUN2-mediated breast cancer progression via
            interaction with ELAVL1
  JOURNAL   Cell Signal 117, 111112 (2024)
   PUBMED   38387687
  REMARK    GeneRIF: LncRNA HOST2 promotes NSUN2-mediated breast cancer
            progression via interaction with ELAVL1.
REFERENCE   5  (bases 1 to 2951)
  AUTHORS   Chen,Y., Jiang,Z., Zhang,C., Zhang,L., Chen,H., Xiao,N., Bai,L.,
            Liu,H. and Wan,J.
  TITLE     5-Methylcytosine transferase NSUN2 drives NRF2-mediated ferroptosis
            resistance in non-small cell lung cancer
  JOURNAL   J Biol Chem 300 (4), 106793 (2024)
   PUBMED   38403250
  REMARK    GeneRIF: 5-Methylcytosine transferase NSUN2 drives NRF2-mediated
            ferroptosis resistance in non-small cell lung cancer.
REFERENCE   6  (bases 1 to 2951)
  AUTHORS   Sakita-Suto,S., Kanda,A., Suzuki,F., Sato,S., Takata,T. and
            Tatsuka,M.
  TITLE     Aurora-B regulates RNA methyltransferase NSUN2
  JOURNAL   Mol Biol Cell 18 (3), 1107-1117 (2007)
   PUBMED   17215513
  REMARK    GeneRIF: These results indicate that Aurora-B participates to
            regulate the assembly of nucleolar RNA-processing machinery and the
            RNA methyltransferase activity of NSUN2 via phosphorylation at
            Ser139 during mitosis.
REFERENCE   7  (bases 1 to 2951)
  AUTHORS   Najmabadi,H., Motazacker,M.M., Garshasbi,M., Kahrizi,K.,
            Tzschach,A., Chen,W., Behjati,F., Hadavi,V., Nieh,S.E.,
            Abedini,S.S., Vazifehmand,R., Firouzabadi,S.G., Jamali,P.,
            Falah,M., Seifati,S.M., Gruters,A., Lenzner,S., Jensen,L.R.,
            Ruschendorf,F., Kuss,A.W. and Ropers,H.H.
  TITLE     Homozygosity mapping in consanguineous families reveals extreme
            heterogeneity of non-syndromic autosomal recessive mental
            retardation and identifies 8 novel gene loci
  JOURNAL   Hum Genet 121 (1), 43-48 (2007)
   PUBMED   17120046
REFERENCE   8  (bases 1 to 2951)
  AUTHORS   Brzezicha,B., Schmidt,M., Makalowska,I., Jarmolowski,A.,
            Pienkowska,J. and Szweykowska-Kulinska,Z.
  TITLE     Identification of human tRNA:m5C methyltransferase catalysing
            intron-dependent m5C formation in the first position of the
            anticodon of the pre-tRNA Leu (CAA)
  JOURNAL   Nucleic Acids Res 34 (20), 6034-6043 (2006)
   PUBMED   17071714
  REMARK    GeneRIF: First report showing intron-dependent methylation of human
            pre-tRNA Leu(CAA) and identification of human gene encoding tRNA
            methylase(Trm4) responsible for this reaction.
REFERENCE   9  (bases 1 to 2951)
  AUTHORS   Ballif,B.A., Villen,J., Beausoleil,S.A., Schwartz,D. and Gygi,S.P.
  TITLE     Phosphoproteomic analysis of the developing mouse brain
  JOURNAL   Mol Cell Proteomics 3 (11), 1093-1101 (2004)
   PUBMED   15345747
REFERENCE   10 (bases 1 to 2951)
  AUTHORS   Beausoleil,S.A., Jedrychowski,M., Schwartz,D., Elias,J.E.,
            Villen,J., Li,J., Cohn,M.A., Cantley,L.C. and Gygi,S.P.
  TITLE     Large-scale characterization of HeLa cell nuclear phosphoproteins
  JOURNAL   Proc Natl Acad Sci U S A 101 (33), 12130-12135 (2004)
   PUBMED   15302935
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BF000644.1, BG387061.1,
            AK298980.1 and BC001041.2.
            
            On May 31, 2019 this sequence version replaced NM_001193455.1.
            
            Summary: This gene encodes a methyltransferase that catalyzes the
            methylation of cytosine to 5-methylcytosine (m5C) at position 34 of
            intron-containing tRNA(Leu)(CAA) precursors. This modification is
            necessary to stabilize the anticodon-codon pairing and correctly
            translate the mRNA. Alternatively spliced transcript variants
            encoding different isoforms have been noted for this gene.[provided
            by RefSeq, Mar 2011].
            
            Transcript Variant: This variant (2) lacks an in-frame coding exon
            in the 5' region compared to variant 1. This results in a shorter
            isoform (2) missing an internal protein segment compared to isoform
            1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR7346977.966089.1, AK298980.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-14                BF000644.1         3-16                c
            15-625              BG387061.1         7-617
            626-2621            AK298980.1         624-2619
            2622-2951           BC001041.2         2399-2728
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5p15.31"
     gene            1..2951
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /note="NOP2/Sun RNA methyltransferase 2"
                     /db_xref="GeneID:54888"
                     /db_xref="HGNC:HGNC:25994"
                     /db_xref="MIM:610916"
     exon            1..161
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /inference="alignment:Splign:2.1.0"
     CDS             66..2264
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /EC_number="2.1.1.203"
                     /note="isoform 2 is encoded by transcript variant 2;
                     5-methycytoisine methyltransferase; Myc-induced
                     SUN-domain-containing protein; NOL1/NOP2/Sun domain
                     family, member 2; tRNA methyltransferase 4 homolog; tRNA
                     (cytosine-5-)-methyltransferase NSUN2; tRNA
                     (cytosine(34)-C(5))-methyltransferase; NOP2/Sun RNA
                     methyltransferase family member 2; substrate of
                     AIM1/Aurora kinase B; myc-induced SUN domain-containing
                     protein; mRNA cytosine C(5)-methyltransferase; tRNA
                     cytosine C(5)-methyltransferase; RNA cytosine
                     C(5)-methyltransferase NSUN2"
                     /codon_start=1
                     /product="RNA cytosine C(5)-methyltransferase NSUN2
                     isoform 2"
                     /protein_id="NP_001180384.1"
                     /db_xref="CCDS:CCDS54832.1"
                     /db_xref="GeneID:54888"
                     /db_xref="HGNC:HGNC:25994"
                     /db_xref="MIM:610916"
                     /translation="MGRRSRGRRLQQQQRPEDAEDGAEGGGKRGEAGWEGGYPEIVKE
                     NKLFEHYYQELKIVPEGEWGQFMDALREPLPATLRITGYKRYPEELAWHTNLSRKILR
                     KSPHLEKFHQFLVSETESGNISRQEAVSMIPPLLLNVRPHHKILDMCAAPGSKTTQLI
                     EMLHADMNVPFPEGFVIANDVDNKRCYLLVHQAKRLSSPCIMVVNHDASSIPRLQIDV
                     DGRKEILFYDRILCDVPCSGDGTMRKNIDVWKKWTTLNSLQLHGLQLRIATRGAEQLA
                     EGGRMVYSTCSLNPIEDEAVIASLLEKSEGALELADVSNELPGLKWMPGITQWKVMTK
                     DGQWFTDWDAVPHSRHTQIRPTMFPPKDPEKLQAMHLERCLRILPHHQNTGGFFVAVL
                     VKKSSMPWNKRQPKLQGKSAETRESTQLSPADLTEGKPTDPSKLESPSFTGTGDTEIA
                     HATEDLENNGSKKDGVCGPPPSKKMKLFGFKEDPFVFIPEDDPLFPPIEKFYALDPSF
                     PRMNLLTRTTEGKKRQLYMVSKELRNVLLNNSEKMKVINTGIKVWCRNNSGEEFDCAF
                     RLAQEGIYTLYPFINSRIITVSMEDVKILLTQENPFFRKLSSETYSQAKDLAKGSIVL
                     KYEPDSANPDALQCPIVLCGWRGKASIRTFVPKNERLHYLRMMGLEVLGEKKKEGVIL
                     TNESAASTGQPDNDVTEGQRAGEPNSPDAEEANSPDVTAGCDPAGVHPPR"
     exon            162..319
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /inference="alignment:Splign:2.1.0"
     exon            320..425
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /inference="alignment:Splign:2.1.0"
     exon            426..497
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /inference="alignment:Splign:2.1.0"
     exon            498..582
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /inference="alignment:Splign:2.1.0"
     exon            583..775
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /inference="alignment:Splign:2.1.0"
     exon            776..850
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /inference="alignment:Splign:2.1.0"
     exon            851..981
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /inference="alignment:Splign:2.1.0"
     exon            982..1055
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /inference="alignment:Splign:2.1.0"
     exon            1056..1186
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /inference="alignment:Splign:2.1.0"
     exon            1187..1283
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /inference="alignment:Splign:2.1.0"
     exon            1284..1468
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /inference="alignment:Splign:2.1.0"
     exon            1469..1561
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /inference="alignment:Splign:2.1.0"
     exon            1562..1697
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /inference="alignment:Splign:2.1.0"
     exon            1698..1778
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /inference="alignment:Splign:2.1.0"
     exon            1779..1917
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /inference="alignment:Splign:2.1.0"
     exon            1918..1957
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /inference="alignment:Splign:2.1.0"
     exon            1958..2951
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2932..2937
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /note="hexamer: AATAAA"
     polyA_site      2951
                     /gene="NSUN2"
                     /gene_synonym="MISU; MRT5; SAKI; TRM4"
                     /note="major polyA site"
ORIGIN      
        1 gtagggctag agttctggcc gtggcgggcc ggtttctgcg tgctgcgtgc gcggccgcgt
       61 gggctatggg gcggcggtcg cggggtcggc ggctccagca acagcagcgg ccggaggacg
      121 cggaggatgg cgccgagggt ggtggaaagc gcggcgaggc gggctgggaa ggaggctacc
      181 ccgagatcgt caaggagaac aagctgttcg agcactacta ccaggagctc aagatcgtgc
      241 ccgagggcga gtggggccag ttcatggacg ctctcaggga gccgctcccg gccactttaa
      301 gaattactgg ttacaaaagg tatcctgaag aacttgcctg gcacacaaat ttaagtcgaa
      361 aaatcttgag aaaatcgcca cacttggaaa agtttcatca gtttctagtt agtgaaacag
      421 aatctggaaa tattagtcgt caagaagctg ttagcatgat cccaccactg ctcctcaacg
      481 tgcggcctca tcataagatc ttagatatgt gtgcagcacc tggctcaaag accacacagt
      541 taattgaaat gctacatgcc gacatgaatg tcccctttcc agagggattt gttattgcga
      601 atgatgtgga caacaagcgc tgctacctgc tcgtccatca agccaagagg ctgagcagcc
      661 cctgcatcat ggtggtcaac catgatgcct ccagcatacc caggctccag atagatgtgg
      721 acggcaggaa agagatcctc ttctatgatc gaattttatg tgatgtccct tgcagtggag
      781 acggcactat gagaaaaaac attgatgttt ggaaaaagtg gaccacctta aatagcttgc
      841 agctacatgg cttacagctg cggattgcaa cacgcggggc tgaacagctg gctgaaggtg
      901 gaaggatggt gtattccacg tgttcactaa accctattga ggatgaagca gtcatagcat
      961 ctttactgga aaaaagtgaa ggtgctttgg agcttgctga tgtgtctaat gaactgccag
     1021 ggctgaagtg gatgcctgga atcacacagt ggaaggtaat gacgaaagat gggcagtggt
     1081 ttacagactg ggacgctgtt cctcacagca gacacaccca gatccgacct accatgttcc
     1141 ctccgaagga cccagaaaag ctgcaggcca tgcacctgga gcgatgcctt aggatattac
     1201 cccatcatca gaatactgga gggttttttg tggcagtatt ggtgaaaaaa tcttcaatgc
     1261 cgtggaataa acgtcagcca aagcttcagg gtaaatctgc agagaccaga gaaagcacac
     1321 agctgagccc tgcagatctc acagaaggga aacccacaga tccctctaag ctggaaagtc
     1381 cgtcattcac aggaactggt gacacagaaa tagctcatgc aactgaggat ttagagaata
     1441 atggcagtaa gaaagatggc gtgtgtggtc ctcctccatc aaagaaaatg aagttatttg
     1501 gatttaaaga agatccattt gtatttattc ctgaagatga cccattattt ccacctattg
     1561 agaaatttta tgctttggat ccttcattcc caaggatgaa tttgttaact cggactacag
     1621 aagggaagaa aaggcagctc tacatggttt ctaaggagtt gcggaatgtg ctgctgaata
     1681 acagtgagaa gatgaaggtt attaacacgg ggatcaaagt ctggtgtaga aataacagcg
     1741 gtgaagagtt tgactgtgct ttccggctgg cacaggaggg aatatataca ttgtatccat
     1801 ttattaactc aagaattatt actgtatcaa tggaagatgt taagatactg ttgacccagg
     1861 aaaatccctt ttttagaaaa ctcagcagtg agacctacag tcaagcaaag gacctggcaa
     1921 agggaagcat cgtgctgaag tatgaaccag attctgcgaa tccagacgct ctgcagtgtc
     1981 ccatcgtctt atgcggatgg cggggaaagg cctccattcg aacttttgtg cccaagaatg
     2041 aacggcttca ttatctcagg atgatggggc tggaggtatt gggagaaaag aagaaggaag
     2101 gggttatcct cacaaatgag agtgcagcca gcaccggaca gccagacaat gacgtgactg
     2161 agggacagag agcaggagag cccaacagcc cagatgcaga agaggccaac agtccagacg
     2221 tgacagcagg ctgtgacccg gcgggggtcc atccaccccg gtgagcaggc ccaaggcagc
     2281 gggggcccac acccctcaca cgcaaaactg gcttcttctg gtcactggtg tctgaaacca
     2341 aatccagagc agcctgtggc ctgtaaagca tatatttcta atgactgcag actggtggga
     2401 tcataggagc cttctgaatg accaggactg ctttctttgg agctgatgaa aatgtactct
     2461 tttagcgtgt tagaaatcac ttgttttatt ttgtttcttt ggccaagctg ggtctagtgt
     2521 ttcttttgct gggaatagac tttcaaaagt tgtacttcta tcaagaaaca aaactgccct
     2581 tgcagaaatt tcaggtcttt tgttaagcct gtattggtct taaggtgcag tattttttaa
     2641 attattattt atagaaagaa tctataaatt cttggggaag tgtgttataa gctttaataa
     2701 ttacattgag ctgcacctca gtggtgtgtc attaacatgc agtggggtta atatctgagg
     2761 cctcagatga ctttgtgcct tttggaataa agggtaaaat aaactctccc agagtaagag
     2821 ctgtatcgtg aattgtcata ctaattattg agggggactt atgtgctttt attgaatgga
     2881 gtgctttaca atttttattt ttaaatgggg ttgggatcct tggaatattt caataaaatt
     2941 gataaaatat a
//
