LOCUS       NM_015471              13115 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens NSL1 component of MIS12 kinetochore complex (NSL1),
            transcript variant 1, mRNA.
ACCESSION   NM_015471
VERSION     NM_015471.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 13115)
  AUTHORS   Trepte,P., Secker,C., Kostova,S., Maseko,S.B., Choi,S.G.,
            Blavier,J., Minia,I., Ramos,E.S., Cassonnet,P., Golusik,S.,
            Zenkner,M., Beetz,S., Liebich,M.J., Scharek,N., Schutz,A.,
            Sperling,M., Lisurek,M., Wang,Y., Spirohn,K., Hao,T.,
            Calderwood,M.A., Hill,D.E., Landthaler,M., Olivet,J., Twizere,J.C.,
            Vidal,M. and Wanker,E.E.
  TITLE     AI-guided pipeline for protein-protein interaction drug discovery
            identifies a SARS-CoV-2 inhibitor
  JOURNAL   bioRxiv (2023)
   PUBMED   37398436
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13115)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 13115)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   4  (bases 1 to 13115)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Paulo,J.A., Cannon,J.R., Ting,L.,
            Baltier,K., Colby,G., Gebreab,F., Gygi,M.P., Parzen,H., Szpyt,J.,
            Tam,S., Zarraga,G., Pontano-Vaites,L., Swarup,S., White,A.E.,
            Schweppe,D.K., Rad,R., Erickson,B.K., Obar,R.A., Guruharsha,K.G.,
            Li,K., Artavanis-Tsakonas,S., Gygi,S.P. and Harper,J.W.
  TITLE     Architecture of the human interactome defines protein communities
            and disease networks
  JOURNAL   Nature 545 (7655), 505-509 (2017)
   PUBMED   28514442
REFERENCE   5  (bases 1 to 13115)
  AUTHORS   Petrovic,A., Keller,J., Liu,Y., Overlack,K., John,J.,
            Dimitrova,Y.N., Jenni,S., van Gerwen,S., Stege,P., Wohlgemuth,S.,
            Rombaut,P., Herzog,F., Harrison,S.C., Vetter,I.R. and Musacchio,A.
  TITLE     Structure of the MIS12 Complex and Molecular Basis of Its
            Interaction with CENP-C at Human Kinetochores
  JOURNAL   Cell 167 (4), 1028-1040 (2016)
   PUBMED   27881301
REFERENCE   6  (bases 1 to 13115)
  AUTHORS   Kiyomitsu,T., Iwasaki,O., Obuse,C. and Yanagida,M.
  TITLE     Inner centromere formation requires hMis14, a trident kinetochore
            protein that specifically recruits HP1 to human chromosomes
  JOURNAL   J Cell Biol 188 (6), 791-807 (2010)
   PUBMED   20231385
  REMARK    GeneRIF: The hMis14, which contains a tripartite-binding domain for
            HP1 and two other kinetochore proteins, hMis13 and blinkin, is a
            cornerstone for the assembly of the inner centromere and
            kinetochore.
REFERENCE   7  (bases 1 to 13115)
  AUTHORS   Kiyomitsu,T., Obuse,C. and Yanagida,M.
  TITLE     Human Blinkin/AF15q14 is required for chromosome alignment and the
            mitotic checkpoint through direct interaction with Bub1 and BubR1
  JOURNAL   Dev Cell 13 (5), 663-676 (2007)
   PUBMED   17981135
REFERENCE   8  (bases 1 to 13115)
  AUTHORS   Kline,S.L., Cheeseman,I.M., Hori,T., Fukagawa,T. and Desai,A.
  TITLE     The human Mis12 complex is required for kinetochore assembly and
            proper chromosome segregation
  JOURNAL   J Cell Biol 173 (1), 9-17 (2006)
   PUBMED   16585270
REFERENCE   9  (bases 1 to 13115)
  AUTHORS   Obuse,C., Iwasaki,O., Kiyomitsu,T., Goshima,G., Toyoda,Y. and
            Yanagida,M.
  TITLE     A conserved Mis12 centromere complex is linked to heterochromatic
            HP1 and outer kinetochore protein Zwint-1
  JOURNAL   Nat Cell Biol 6 (11), 1135-1141 (2004)
   PUBMED   15502821
REFERENCE   10 (bases 1 to 13115)
  AUTHORS   Cheeseman,I.M., Niessen,S., Anderson,S., Hyndman,F., Yates,J.R.
            3rd, Oegema,K. and Desai,A.
  TITLE     A conserved protein network controls assembly of the outer
            kinetochore and its ability to sustain tension
  JOURNAL   Genes Dev 18 (18), 2255-2268 (2004)
   PUBMED   15371340
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK223260.1, AL591647.14 and
            AA806390.1.
            
            On Nov 22, 2018 this sequence version replaced NM_015471.3.
            
            Summary: This gene encodes a protein with two coiled-coil domains
            that localizes to kinetochores, which are chromosome-associated
            structures that attach to microtubules and mediate chromosome
            movements during cell division. The encoded protein is part of a
            conserved protein complex that includes two chromodomain-containing
            proteins and a component of the outer plate of the kinetochore.
            This protein complex is proposed to bridge centromeric
            heterochromatin with the outer kinetochore structure. Multiple
            transcript variants encoding different isoforms have been found for
            this gene. There is a pseudogene of the 3' UTR region of this gene
            on chromosome X. [provided by RefSeq, Jul 2014].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074967.2526052.1,
                                           SRR14038191.280817.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000366977.8/ ENSP00000355944.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1625              AK223260.1         1-1625
            1626-12762          AL591647.14        52759-63895         c
            12763-13115         AA806390.1         14-366              c
FEATURES             Location/Qualifiers
     source          1..13115
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q32.3"
     gene            1..13115
                     /gene="NSL1"
                     /gene_synonym="C1orf48; DC8; MIS14"
                     /note="NSL1 component of MIS12 kinetochore complex"
                     /db_xref="GeneID:25936"
                     /db_xref="HGNC:HGNC:24548"
                     /db_xref="MIM:609174"
     exon            1..248
                     /gene="NSL1"
                     /gene_synonym="C1orf48; DC8; MIS14"
                     /inference="alignment:Splign:2.1.0"
     CDS             15..860
                     /gene="NSL1"
                     /gene_synonym="C1orf48; DC8; MIS14"
                     /note="isoform 1 is encoded by transcript variant 1;
                     kinetochore-associated protein NSL1 homolog; NSL1, MIND
                     kinetochore complex component, homolog; NSL1, MIS12
                     kinetochore complex component"
                     /codon_start=1
                     /product="kinetochore-associated protein NSL1 homolog
                     isoform 1"
                     /protein_id="NP_056286.3"
                     /db_xref="CCDS:CCDS1509.1"
                     /db_xref="GeneID:25936"
                     /db_xref="HGNC:HGNC:24548"
                     /db_xref="MIM:609174"
                     /translation="MAGSPELVVLDPPWDKELAAGTESQALVSATPREDFRVRCTSKR
                     AVTEMLQLCGRFVQKLGDALPEEIREPALRDAQWTFESAVQENISINGQAWQEASDNC
                     FMDSDIKVLEDQFDEIIVDIATKRKQYPRKILECVIKTIKAKQEILKQYHPVVHPLDL
                     KYDPDPAPHMENLKCRGETVAKEISEAMKSLPALIEQGEGFSQVLRMQPVIHLQRIHQ
                     EVFSSCHRKPDAKPENFITQIETTPTETASRKTSDMVLKRKQTKDCPQRKWYPLRPKK
                     INLDT"
     exon            249..327
                     /gene="NSL1"
                     /gene_synonym="C1orf48; DC8; MIS14"
                     /inference="alignment:Splign:2.1.0"
     exon            328..458
                     /gene="NSL1"
                     /gene_synonym="C1orf48; DC8; MIS14"
                     /inference="alignment:Splign:2.1.0"
     exon            459..513
                     /gene="NSL1"
                     /gene_synonym="C1orf48; DC8; MIS14"
                     /inference="alignment:Splign:2.1.0"
     exon            514..581
                     /gene="NSL1"
                     /gene_synonym="C1orf48; DC8; MIS14"
                     /inference="alignment:Splign:2.1.0"
     exon            582..13115
                     /gene="NSL1"
                     /gene_synonym="C1orf48; DC8; MIS14"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1489..1494
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NSL1"
                     /gene_synonym="C1orf48; DC8; MIS14"
                     /note="hexamer: AATAAA"
     polyA_site      1511
                     /gene="NSL1"
                     /gene_synonym="C1orf48; DC8; MIS14"
                     /note="major polyA site"
     regulatory      1626..1631
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NSL1"
                     /gene_synonym="C1orf48; DC8; MIS14"
                     /note="hexamer: AATATA"
     polyA_site      1649
                     /gene="NSL1"
                     /gene_synonym="C1orf48; DC8; MIS14"
     regulatory      8316..8321
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NSL1"
                     /gene_synonym="C1orf48; DC8; MIS14"
                     /note="hexamer: AATGAA"
     polyA_site      8338
                     /gene="NSL1"
                     /gene_synonym="C1orf48; DC8; MIS14"
     regulatory      13088..13093
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NSL1"
                     /gene_synonym="C1orf48; DC8; MIS14"
                     /note="hexamer: AATAAA"
     polyA_site      13115
                     /gene="NSL1"
                     /gene_synonym="C1orf48; DC8; MIS14"
ORIGIN      
        1 agttccgacg aaaaatggcg gggtctcctg agttggtggt ccttgaccct ccatgggaca
       61 aggagctcgc ggctggcaca gagagccagg ccttggtctc cgccactccc cgagaagact
      121 ttcgggtgcg ctgcacctcg aagcgggctg tgaccgaaat gctacaactg tgcggccgct
      181 tcgtgcaaaa gctcggggac gctctgccgg aggagattcg ggagcccgct ctgcgagatg
      241 cgcagtggac ttttgaatca gctgtgcaag agaatatcag cattaatggg caagcatggc
      301 aggaagcttc agataattgt tttatggatt ctgacatcaa agtacttgaa gatcagtttg
      361 atgaaatcat agtagatata gccacaaaac gtaagcagta tcccagaaag atcctggaat
      421 gtgtcatcaa aaccataaaa gcaaaacaag aaattctgaa gcagtaccac cctgttgtac
      481 atccactgga cctaaaatat gaccctgatc cagcccctca tatggaaaat ttgaaatgca
      541 gaggggaaac agtagcaaag gagatcagtg aagccatgaa gtccttgcct gcattaattg
      601 aacaaggaga gggattttcc caagttctca ggatgcagcc tgttatccac ctccagagga
      661 ttcaccaaga agtcttttcc agttgtcata ggaaaccaga tgctaaacct gagaacttta
      721 taacacagat agaaaccaca ccaacagaga ctgcttccag gaaaacctct gacatggtac
      781 tgaaaagaaa gcaaactaaa gactgccccc agagaaaatg gtatccattg cggccaaaga
      841 aaattaatct tgatacatga gctctttctg tttattttgg gagttgaaaa taggcaccat
      901 caacatttag attacagcct aattaatacc tagataagac ttcatttgaa ataagaaata
      961 actcttttac tagtgattca tttatacaga tatagtatct ctgtgcgggg atatgatata
     1021 atattgtatt tccttactgt tttatctatt gtaaataaaa agcattttaa aaagtattga
     1081 cacaaagccc atcagtgggc attaaaaata ttaaaagtgc agacttttac tgtccttaag
     1141 tgccatcaac tctcagctcc cttgtagctt ttgtgggatt taacaagtaa caaattctgt
     1201 tgtgtttccc tggtatacat ctttctagga aaaaaaaaaa aaagagagag agctgtataa
     1261 tgatttttcg tttacatgct gaaaagtaat tatcagttct gcacagcagc agatgcaggg
     1321 ttttttttta aagatgtagt ttgatttatc aaattaatgt gctgatgata atactggctt
     1381 tgactttgtt actccatgtt cagctaattt aggtttgtga gattaacttt aggatttttt
     1441 gttgtgtaag acaatgataa ctattatttg tgcaacatta ctctttgaaa taaaaattgg
     1501 catgtagcca atgtttcctg cccacactca cttttttcta tagaccatta acataatttg
     1561 acttggaact aatggtttct ttttagggtt tcttatttat ttctttacaa atcattccag
     1621 ttcaaaatat atatcagatt aatacactgg caaagtgtca ttgtgttatt gaactatttc
     1681 acatttgaag atgtttatat atcaggtata taatcactgg gagaagtctg agaaaacgat
     1741 attggcttta cttctaacat ttaactatag tttcttatag ctcttattgt gtgtcttaca
     1801 ttatacagtg taggcagggc catcaaactt accaactgtt tccccttttg atgactctga
     1861 taccttcatt catttttcaa aggaatttac tgatcatgag gttggaaaat ctgtatttct
     1921 cttgcttatt atgtattaat aatcataaat gtctagattc accagaagtc accagaaggt
     1981 ctgtctcagt gaagaaaact tataaagcca ctttgttgca ttttgtgttt cagtgttaca
     2041 gtttgagatc tgtatatttg tacacagcta tgtgtttttc attgaaataa tgtacaaaga
     2101 ctgatcttga tgctgtgtat ttttatgagt tgtcttaggc attcctgagc tcagcttcag
     2161 ttggatggtg ggtcagcacc ctgcgtttct gaacatacta gacttcagtt taaaactgtt
     2221 ttgaggctgt ataatatgtt gctgttttta cagtgctaat gtttttatag tattaaaatt
     2281 ctgtatgatt tgtttcactt cagaggtccc caaacaaaag agttattgtt aattctatta
     2341 gattatataa gctacttgca ttttgattat ataagaacat ggtccctccc ttttaagaga
     2401 ggtatattgt gctacataga gtttttaaat gacatgatag atttgctctg aagtattcag
     2461 cagagaaaca aggaatcgat taaggaaatg tgacaaaaat cctgttaatg ttgaatctgt
     2521 gtaatgacta tattctactt tgtgttggaa atttttataa gtttttaaaa aatggttata
     2581 ttttaaagtc cccaggcaga cagctgagat ttgaaatcct cttgagagcc caaagtttta
     2641 ctccatagtt atagcagagt ttaaaataga aataccactg ggaaagacag tacgtattag
     2701 cattattaac taagaaaagc atgaccaata gcagtaataa tattgaataa gtatactgtg
     2761 taagaggaga agttgcattt ctgcataggc caagaactag taagctaagg attaaaagca
     2821 agaaagtctg tagttttaag gaattggaaa ggttgaagga aaaaattgaa aatcaaatct
     2881 tgctctatga taaaaagagt ctggattgaa tagggtctta aaattatctt tcagaaaaga
     2941 aatagccagg cgcggtgggc tcatgcctgt aatcccagca ctttgggagg ctgaagtgga
     3001 cagattactt gagcccagcc tgggcaacat agtgaaactc catttctaca aaaaatacaa
     3061 aaattagcag ggtgtgatgg cacacacctg tagtcccagc cacccaggag gctgaaatga
     3121 gaggatcact tgagccctgg aggcagaagt tgcagtgagc caggatggca ccactgtact
     3181 ccagcctggg tgacagagtg agaccctgtt tcaaaataat aataagaaga acacaagaag
     3241 gttaagtcta gtcccaaaga agagagaaca ctgtcaaagc tggtttctga aaactggtac
     3301 caaaatctac atttgtttat ttaggctact ataacagaat accacaggct gggtggctta
     3361 aacaacagat acttacttct cacagttctg gaggctagaa gtccatgaga aggtgctggc
     3421 aaatgcagtt tctggtgagg gctgtcttcc cgacttgtag atgaccacct ccttactatg
     3481 tcctcacatg gcctttcctc agtgcatgca tgtggagaga gggacagagt gcaagctctc
     3541 tggtgtctct tcttataagg acaccaattg tatcagatca gagccccacc cttatggcct
     3601 catttaacct taattatttc cttagaggcc ccatctccaa atacagctat aggcacatgg
     3661 gagttaaggc ttcatcatac agattttagg gggacacaaa cattgagtcc ataacacaag
     3721 cccacagaaa atcttatcct agacctcttt ccctggccct ttatggcttg ggctccgtag
     3781 aggacaaagt gaataaaacc aaatttttta ttgcttcaaa cagtatgatt tcatcttgta
     3841 agatgtactt tttcttttct aaggctgaat ttgtttgatt tctggtgaac acctatcttc
     3901 tctatccacc ttcattcatt tattcatctg ttcaacacat acagttggac acctactcgg
     3961 atcaaaagtc agagatctta agagcacagc ctctaagcca gatcatctga gttcagattt
     4021 cagttctacc attattagtt caatgatatt ggacaagtta cttaacctgt ctatgatcaa
     4081 aagtcagaga tcttaaagag catagtctct gagccagatc atctgggttc agattgcagt
     4141 tctaccatta ttagttcagt gacattgggc aagttacata acctgtctat gctgcagttt
     4201 tctcatgtgc aaaatgggga taataattat gcctacctca tagcgttatt ctgagaagta
     4261 aatgagttca taatgtgaag gccttagact aataactggt gcatatgagt actctatgat
     4321 cactgttatt acttcttttg ataaaggaaa tgaaaacact caggacaagt gttgccttca
     4381 ggaaggtcat attccagtga agaggatgag tgtgcaaaca atgataatgc catgcagcaa
     4441 atggtatgat agatgtatgt gtggaactta ggggagtagt catggaagtc tttgtagaag
     4501 aggtggcatt tgagcagtct tgaatgtttt aggcaaataa tcagggaagg aatacaggaa
     4561 taaagaacaa attactgtta taacaatgtg gatcaatatc aaagatacta tattgaataa
     4621 aagaagcaaa gccaggcaca agagaataca tcctgtgtat ggaatcacag aatgggcaaa
     4681 actaagccat ggtggttccc tctggggaga ggggagatat taactgggaa tggcacggga
     4741 aacatttttg ggtgagcaaa atgttctgta tcttgatctg agtggtgctc acatggctgt
     4801 atatgtatgt aaaattttgt tgaactatac acttaaaatt tctgcacttt atttaagttt
     4861 tatattttaa aaatgcacaa aactgattca ccaaatattt gaggagcgtc tccagcatca
     4921 aagacagacc caaaaactgt aagtccctta atggcaggaa tctttgttcc atccacctgg
     4981 aataattgct ggcacatgat gggccctcga tgcatagtgg ttaactgagt gaattaaaac
     5041 aaaaaccaaa gagaataaag gacctcaaag aaaacagata catacaaagc aaaatcccag
     5101 aatgatgact gtactcccag aaagaagaga aaatcataca ttttttggaa aatcggaatg
     5161 ctattaaata atgttcagta aataagaaaa agtttttgaa aattaaaaat atgaaagttg
     5221 aaataaaaat tcagtgggag ggctgggaga taaaatgaaa ttttcccaaa aataaaacag
     5281 aaagattaag aaatggcaaa ttagaaaagt aagaataaag agaataagtt cataaagttc
     5341 aaagtctaat cgatacccat tccagagata gcatgcagaa aaagcagcag agtcaaaatt
     5401 atcaaagaga taatgcagtt accatatggc ccagcaattt tactccacgt tctaaaccta
     5461 agagaattga aaacatgctc acacaataac ttacacacaa atgtttgtag cagcaggttg
     5521 aactttcatg aactgatgaa tggataaata aaatgtggac tatccactca atagaatatt
     5581 atttggcaat aagaagaaat gaggtactgg taaatgccac agtgtagatg aaccttaaaa
     5641 catgccaagt gaaagaagcc tctcacaaaa gactgctgaa ttttgtagac tccatgtaaa
     5701 tgaactgtcc agaacaggca aatctacaga aacaaaaaat agatctgtca ctgcctaagg
     5761 ctggaaagat ggagagggtg gaaggaatag gaagtgactg ctaatgggta tgggattttt
     5821 tttttttttt ttttgtgaaa caaggtcttg ctgtgttgcc caagctggag tacagtggtg
     5881 ctatcttggc ttactgcaac ctctgccccc gcaactcaag ccatcctccc accttagcct
     5941 cccaagtagg tgggactata ggcatgcacc atcatgccca gctaattttt gtattttttg
     6001 tagagacgga gtttcgctat gtggctggtc tcaaactcgt gagctcaagc gatccgccca
     6061 cctctgcctc ccaaagtgct ggattacagg cgtgagccac tgcgcctggc ctgggatgtc
     6121 ttttgagggt gatgaaaacc ctctaaattt gattatggtg attcacgact gcttgttgaa
     6181 tatactgaaa accattgaac aatacagttt aaaagggtga gtgagatggt aaagtatatc
     6241 tcaatttaaa aagagaataa agaaatagaa ataacacaag aaacattccc agacctgagg
     6301 aataccaaga ggaattggaa tggcatcaga ttactaaaca acaatcctaa aatctagaag
     6361 catctggaga aatattctca gcataaatga tatacaacct aggcttttat acacagtcaa
     6421 actaataatc aagtgcatga ttagctaaaa gatatctcag atatgcaaag cgtcaaaaaa
     6481 aattacctcc aaggcatccc gtttgggggt tctaccaggg gctgtgctct attccaaagg
     6541 gtaaaccaaa aaagcagcag ccatgggatc tgggaaacta aggctcccaa aacacagcac
     6601 actcgagtct acaggctact aactaatcag cagatttgga gactaggcag accaggttgg
     6661 aataagacta tgtaggccgg gtgcggtggc tcacacctgt aatcccagca ccttgggagg
     6721 ccgagacagg cagatcacct gaggctggga attggagacc agcctgacca acaactaaaa
     6781 atacaaaatt agctgggcgt ggtggcgcat gcctgtaatc ccagctactc aggaggctga
     6841 ggctggagaa tcgcttgaac ccaggaggca gaggttgcag tgagccaaga tcacgccatc
     6901 gcactccagc ctgggcaaca agaacgaaac ttcatctcaa aaaaaaaaaa aggactatgt
     6961 aggactccaa aagagatatt ttcagaaaaa aataaaagtg acaacacgag tgataatgtt
     7021 aactatgcag aaaattgtat aaaggagatg ttttgtaata tgattaagat gttcaaagaa
     7081 acaaaaaata aggcagttat tcactacagt agaggcagaa agctgtacaa aaaaagaaat
     7141 gtaatcatgg ttacaaatta gttctccagt gaacaatatt tatatagcca taataataaa
     7201 aaccctgaat ctgaattcag taaaaaaaaa aaaaaaaaat tgggacagtt acactgggag
     7261 gctaaaggat gggaattagc aattagtaca gcacaggcac tgaacagtta gatggacatt
     7321 ggttaaagca gtaaggtggg gtgtggaggc ccttatagct gctgggtcct ggggcagggt
     7381 agggtgtccc aggacatgga gctggagcag ccagagtggc agggatagtg aggaggcttc
     7441 tgtgccaacc agtacaatcc atatgtttgt aacagtgtga ctgtctgaac accagctttg
     7501 acatggcagt ggggagtgaa atcttcaccc accacaactg gaagtcagtg gatgtctaaa
     7561 aagctgattc aagatcatac aatatggata taatttagtg cagcagaata aagagctgac
     7621 gaagtgggaa gtattaactc tgtggatgaa cttaggtggg ggaactgggc cagggactga
     7681 taatttttga tagaagcctc atagatttga tttttaaaat taaatgcata tagtaattaa
     7741 gggaaaataa atttggttta agagacaaat catcccctag aacctttctc ggggtttcag
     7801 tttttcaggg tttcagaagc tttctcaggg tttcaaaaca ggcgtgtgcc accatgcccg
     7861 gctagtttat ttgttttaga gatggggtct cgccatattg ccccggctgg tcttgaattc
     7921 ctgggctcaa gccatcctcc tgcctcagct tcccaaagtg ctgggattac aggaatgagc
     7981 cactgcacct ggcctatcac agtatatttc agataagact tactattctc tgtttttggc
     8041 tgataattct agttttggtt tcttcaggtt tttttttttt tctcccctct gaccatttgt
     8101 tttgcaaata gattatatta tgaaagtctc tgaagaaaaa aatatctctc tagagaacca
     8161 ctgaaggggg ttcttgggga ttttatatga aaggatttga gaaaattaat gttatattca
     8221 tttgaagctt caggggtaaa gaatcccttg taattcgttg ctgtgtctct acattgctca
     8281 tactttgccc atgggctgta aactaatatt cgttgaatga atgagttcat attgcaaatt
     8341 tctaacttgt cggtttccct ggatcacaat ctcacacatt aatggcttgt ggcatttata
     8401 ttcattatta attgaactta gaaggtgagt gtggggaggg gagaggtaaa aaactgagga
     8461 cagatcactg ggaaactgac ctgaattagg actccagagt ctcctgtcta ctagaactac
     8521 gtctactaga actaagtcta attcccatat ccctgcagga cataagagca gaggatggca
     8581 ttgacatccc tcagtgacat cactgtgcta gcaggtatag ttgccttctg atcacctttt
     8641 cctttggtca catcccctga cttctgggtg gcctgggata gctagagaag aaaattcaac
     8701 ttctccctgc aggtcttctg ctccagctga ctcctgtgcc tgtgtaaaag attgggcatt
     8761 tctatcatca ccttgactag ccttactcag agaatggctc cagtgccttt gggctcatac
     8821 ctagttgcag attttaagac ctcccttggt gcccattcat gtagctctgt ggcccacatc
     8881 ttctacaatg atgtcattgg agttcttttg tccatttctg cccctgatgc caccttgatg
     8941 ggaatgccac cctccacaca actcttcacc agtccataaa tacctgagac ttggcgcatt
     9001 tctttttttt tttttttttt tttttttttt gagactgtca cccaggctgg agtgtagtgg
     9061 agcgatctca actcactgca acctccacct cccaggttca agaggttctc atgccttagc
     9121 ctcccgagta gctgggatta caggcgcgtg ctaccatgct gggctaatat ttgcattttt
     9181 atagagatga ggttttgcca tgttggtcag gctggtctcg aactcctgac ctcaggtgat
     9241 ccactcgccc tggcctccca aagtgctgtg attacaggag tgagccgcca cgcccggccc
     9301 atttcattct taaaaaaaaa aatccttccg gaggtttagt tcctccacaa ggcagcttct
     9361 ggttacctgc cccctcaggg tccttgctgc ctgggtcacc ccagcaaccc cttcgtccac
     9421 acatcactgc cctttgcagc accagctgat tacattgctt tttccatgtt ctagctgttc
     9481 ttttgagccg ttttatagcc gagcatctcc accagaagag cggataggaa aaaggaaaag
     9541 agaggaggat ttgaccttgg tatcttgctt catttccttc tctgacaaaa atttggtgaa
     9601 aaggttaaaa taagtgttcg aattctctct gacttgtcat attctgtcta tgccctgtgt
     9661 gcctgcctct gatcctgagg aagtgtagaa atgaggggat gacactggca gaataataaa
     9721 aatgggcaaa gttgtagggc acccctgctc caggtcccat acaccccagc tccagagcct
     9781 caggatcttt cccagccatg tgccagacaa tattgtaaaa tgatttttcg tagccacacc
     9841 ttttattaca cacatactcg atgaatctaa atactgtgct aagagctgtg tggatgtaca
     9901 cctgtaaacc aaggcacaga ccctgcccac aagcaatttg ccgtcagtta ggggagccat
     9961 cagtagcatc tagggatcca ttagagggaa aagagggagc tgagggagag gctaggagcc
    10021 caagcaaacc tgcacttctg ctcctagagg gaacctgaga gtgaaactca tgggtttgga
    10081 aacttctgtg gaattacctg cttgcaagaa atgattttgt ctggttttag aaactacgat
    10141 ggaacactaa tatattcctg taattgactg gcaagtttta acacttactg caatttattt
    10201 ttcttgtaag cccaacccaa gtattcttcc tttaaaaaaa atttcagatt gacaaatgac
    10261 tgcttcttta gaaatcctcc ttactaaaat tactcctcat ttctttttct ttcatattca
    10321 aagggaacaa acgttcaaac acacaaataa acactgctct ttgccagagt ggtggactct
    10381 ggcctcccca cccccagcac tcccagtctt gccctcctgc aaacactaga cttaattttc
    10441 tctgcgctga gggccctcct cgagtcagag tgagaggaaa aacagaaggg agaagctgcg
    10501 agatgtggca acaaagggga cggtgatcag gtttatccct gatgttgtct gtgaaggtgt
    10561 tccattctcc agagatctca ccaaatggta taagtgatgt gcagataagc ctgtggttcc
    10621 cttcactcat cagatcagag cctcagaatt ctcagaaacc atttttttcc taacataaaa
    10681 ctccttttct catttctatc cctctgtaaa tctgatttga aaaaattgtt tgaacaaacc
    10741 aacaatgtca gatttgttcc agctatatga gctcacaatt catctactct tggtagttca
    10801 agataaaaga ttacccaaag aaaaggaaga gattgagtaa agccacaact gtaatagtgt
    10861 cccagattgg gtaaaaggcc attaataaat agcgtgaatt gtagaactgt ttaaagcagc
    10921 atatttttaa aacaggcata taaagaatcc actttatgcc tggaattgta caaaaatact
    10981 ttaaataaat gtttacatat ataatgttat caacttattt gctgaaggta atatcaatat
    11041 aatgctagta gttaccatgt attgagtgct tactacatgc catgatcatg ctaaaagctt
    11101 cacatgcacc atctctttta atcctcaaga caatccatga attaagggct attaccacgc
    11161 ccatttcatg agaaagaggt ggagatgaga agggaagtgc ctggccacct tcccagcggt
    11221 ctatagacca tgtagagcag agcgaggcct tctgaccaca aactctgctt ccaccttctc
    11281 cccgtggttc acagcaaagg ccttgtccag agtctcagac gctctcaccg ctacaccaca
    11341 gcgacctcgt tggttcttct catttttttt caatgacaca tagcaacaga gagtatcaac
    11401 aaacattttt aaatttccca gcagttaacg aatagtgaat attactatgt aatactagtg
    11461 tctaagaaag tgctcaaagt tgtttaaagc aatttattat cttaatgagg ttaaatatga
    11521 acttttgctg ttaatttctt gggaaatata aatcatcata gctagtcatt ctttcatcgt
    11581 gggaagctaa cagtcctgat tgttcaggat tataattata gtaaattctg aatttgtgac
    11641 gtgtgaattt tagaaggtta caaaacaact tccagacaac tgagtcataa agtttttgag
    11701 agaagtatgt ggcacacgtc taattcattg tcctttctct ccagtggtat agttatgata
    11761 ttaacctaaa ttgcaaaagg aatcgtttta gttgactgct gcttgaaatt gtgtcacttt
    11821 tccaaattta ggtaatgtct gttacaagga gtatattttg gaaacattaa cttgactgat
    11881 acttatttcc ctggttaagt gaccttcctg ccatgtgctg agtgccagga cttactgccc
    11941 ctcagtaatt tggaagggga tcctgggccc tgcctggaac aaagggttgt gaaacagctc
    12001 tgagtctcaa ggcaagtctt gaaagccata tatagttttc agaaaaagag atcttctaaa
    12061 ttccattcac ccccatttaa cacattagga gccaagcctg gtggactagc tctaggggca
    12121 gtttaggaga gaaggggatc ggggaacctt catcccttct gcctctggtg aactggcaag
    12181 atgaagagga tgaagctggg tgacccagag cccgccctcc ctctctcggc agtcatctcc
    12241 aaggccggag tagactggac tggagggcct ctgagaggac acagccagga agcacactct
    12301 ccctgtgctc ttcacccacc atgaagagac aggacgggaa agtccgtgct gtgctggtgt
    12361 ccatcagagg gagggacacc atccagcccc tgggaccggg ctcggctctg ttgttgcaca
    12421 gaagtcattt ggagatcccc acgtgccaca tggaggattg tgggaggacc ccaccgaggt
    12481 ggctggcagc ctctcatgga caccatggag agtgacccaa tcactctgca tttgaggact
    12541 gcagagtggc tcagcggtct ggacattgaa gaggagccaa cctcaggggt aacacagtgc
    12601 cgctgggagc tgagatgagc caggtggtcg gtgggggaca caagcctaga acaaattcaa
    12661 taaaccagcc acagacgtta tcatcagatg tgcccagaga caaggtggac aggggcattt
    12721 gctgtggaga ccagagggcc agagagagta cagggacctg gatagatggc tccagcacct
    12781 cttcttctcc ctacacccat atgccatctt gataaacagc agggaaatct gaaaaatacc
    12841 aagtcttccc atagggacag tgttacctcc caaaaactgt ttgaataatt ggcttgaact
    12901 ggattttctg aactgaacta atgctattgt tttgtgggtt tttttcttcc tactgagtag
    12961 agtagttgtt tatgggggac atctgatcct ttcattttct tccagagagc taaccacctg
    13021 ttctgacacc atttattgaa tagtacctct tttcccccac tgatgtgaga ggctgcattt
    13081 ttcatctaat aaaatcgaat atatatttag atcta
//
