LOCUS       NM_016143               2721 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens NSFL1 cofactor (NSFL1C), transcript variant 1, mRNA.
ACCESSION   NM_016143
VERSION     NM_016143.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2721)
  AUTHORS   Xue,X., Duan,R., Zheng,G., Chen,H., Zhang,W. and Shi,L.
  TITLE     Translocator protein (18 kDa) regulates the microglial phenotype in
            Parkinson's disease through P47
  JOURNAL   Bioengineered 13 (4), 11061-11071 (2022)
   PUBMED   35475466
  REMARK    GeneRIF: Translocator protein (18 kDa) regulates the microglial
            phenotype in Parkinson's disease through P47.
REFERENCE   2  (bases 1 to 2721)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   3  (bases 1 to 2721)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 2721)
  AUTHORS   Kaneko,Y., Shimoda,K., Ayala,R., Goto,Y., Panico,S., Zhang,X. and
            Kondo,H.
  TITLE     p97 and p47 function in membrane tethering in cooperation with FTCD
            during mitotic Golgi reassembly
  JOURNAL   EMBO J 40 (9), e105853 (2021)
   PUBMED   33555040
  REMARK    GeneRIF: p97 and p47 function in membrane tethering in cooperation
            with FTCD during mitotic Golgi reassembly.
REFERENCE   5  (bases 1 to 2721)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   6  (bases 1 to 2721)
  AUTHORS   Uchiyama,K., Jokitalo,E., Lindman,M., Jackman,M., Kano,F.,
            Murata,M., Zhang,X. and Kondo,H.
  TITLE     The localization and phosphorylation of p47 are important for Golgi
            disassembly-assembly during the cell cycle
  JOURNAL   J Cell Biol 161 (6), 1067-1079 (2003)
   PUBMED   12810701
REFERENCE   7  (bases 1 to 2721)
  AUTHORS   Uchiyama,K., Jokitalo,E., Kano,F., Murata,M., Zhang,X., Canas,B.,
            Newman,R., Rabouille,C., Pappin,D., Freemont,P. and Kondo,H.
  TITLE     VCIP135, a novel essential factor for p97/p47-mediated membrane
            fusion, is required for Golgi and ER assembly in vivo
  JOURNAL   J Cell Biol 159 (5), 855-866 (2002)
   PUBMED   12473691
REFERENCE   8  (bases 1 to 2721)
  AUTHORS   Meyer,H.H., Wang,Y. and Warren,G.
  TITLE     Direct binding of ubiquitin conjugates by the mammalian p97 adaptor
            complexes, p47 and Ufd1-Npl4
  JOURNAL   EMBO J 21 (21), 5645-5652 (2002)
   PUBMED   12411482
REFERENCE   9  (bases 1 to 2721)
  AUTHORS   Rabouille,C., Kondo,H., Newman,R., Hui,N., Freemont,P. and
            Warren,G.
  TITLE     Syntaxin 5 is a common component of the NSF- and p97-mediated
            reassembly pathways of Golgi cisternae from mitotic Golgi fragments
            in vitro
  JOURNAL   Cell 92 (5), 603-610 (1998)
   PUBMED   9506515
REFERENCE   10 (bases 1 to 2721)
  AUTHORS   Kondo,H., Rabouille,C., Newman,R., Levine,T.P., Pappin,D.,
            Freemont,P. and Warren,G.
  TITLE     p47 is a cofactor for p97-mediated membrane fusion
  JOURNAL   Nature 388 (6637), 75-78 (1997)
   PUBMED   9214505
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB173022.1, AK315433.1,
            AL109658.5 and AA948047.1.
            
            On Aug 11, 2020 this sequence version replaced NM_016143.4.
            
            Summary: N-ethylmaleimide-sensitive factor (NSF) and
            valosin-containing protein (p97) are two ATPases known to be
            involved in transport vesicle/target membrane fusion and fusions
            between membrane compartments. A trimer of the protein encoded by
            this gene binds a hexamer of cytosolic p97 and is required for
            p97-mediated regrowth of Golgi cisternae from mitotic Golgi
            fragments. Alternative splicing results in multiple transcript
            variants. A related pseudogene has been identified on chromosome 8.
            [provided by RefSeq, May 2011].
            
            Transcript Variant: This variant (1) encodes isoform a.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074967.2366121.1,
                                           SRR18074968.474291.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000216879.9/ ENSP00000216879.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-6                 DB173022.1         538-543
            7-1138              AK315433.1         1-1132
            1139-2334           AL109658.5         65555-66750         c
            2335-2721           AA948047.1         8-394               c
FEATURES             Location/Qualifiers
     source          1..2721
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20p13"
     gene            1..2721
                     /gene="NSFL1C"
                     /gene_synonym="dJ776F14.1; P47; UBX1; UBXD10; UBXN2C"
                     /note="NSFL1 cofactor"
                     /db_xref="GeneID:55968"
                     /db_xref="HGNC:HGNC:15912"
                     /db_xref="MIM:606610"
     exon            1..130
                     /gene="NSFL1C"
                     /gene_synonym="dJ776F14.1; P47; UBX1; UBXD10; UBXN2C"
                     /inference="alignment:Splign:2.1.0"
     CDS             26..1138
                     /gene="NSFL1C"
                     /gene_synonym="dJ776F14.1; P47; UBX1; UBXD10; UBXN2C"
                     /note="isoform a is encoded by transcript variant 1; SHP1
                     homolog; p97 cofactor p47; UBX domain-containing protein
                     2C; NSFL1 (p97) cofactor (p47)"
                     /codon_start=1
                     /product="NSFL1 cofactor p47 isoform a"
                     /protein_id="NP_057227.2"
                     /db_xref="CCDS:CCDS13015.1"
                     /db_xref="GeneID:55968"
                     /db_xref="HGNC:HGNC:15912"
                     /db_xref="MIM:606610"
                     /translation="MAAERQEALREFVAVTGAEEDRARFFLESAGWDLQIALASFYED
                     GGDEDIVTISQATPSSVSRGTAPSDNRVTSFRDLIHDQDEDEEEEEGQRFYAGGSERS
                     GQQIVGPPRKKSPNELVDDLFKGAKEHGAVAVERVTKSPGETSKPRPFAGGGYRLGAA
                     PEEESAYVAGEKRQHSSQDVHVVLKLWKSGFSLDNGELRSYQDPSNAQFLESIRRGEV
                     PAELRRLAHGGQVNLDMEDHRDEDFVKPKGAFKAFTGEGQKLGSTAPQVLSTSSPAQQ
                     AENEAKASSSILIDESEPTTNIQIRLADGGRLVQKFNHSHRISDIRLFIVDARPAMAA
                     TSFILMTTFPNKELADESQTLKEANLLNAVIVQRLT"
     exon            131..228
                     /gene="NSFL1C"
                     /gene_synonym="dJ776F14.1; P47; UBX1; UBXD10; UBXN2C"
                     /inference="alignment:Splign:2.1.0"
     exon            229..303
                     /gene="NSFL1C"
                     /gene_synonym="dJ776F14.1; P47; UBX1; UBXD10; UBXN2C"
                     /inference="alignment:Splign:2.1.0"
     exon            304..469
                     /gene="NSFL1C"
                     /gene_synonym="dJ776F14.1; P47; UBX1; UBXD10; UBXN2C"
                     /inference="alignment:Splign:2.1.0"
     exon            470..562
                     /gene="NSFL1C"
                     /gene_synonym="dJ776F14.1; P47; UBX1; UBXD10; UBXN2C"
                     /inference="alignment:Splign:2.1.0"
     exon            563..672
                     /gene="NSFL1C"
                     /gene_synonym="dJ776F14.1; P47; UBX1; UBXD10; UBXN2C"
                     /inference="alignment:Splign:2.1.0"
     exon            673..810
                     /gene="NSFL1C"
                     /gene_synonym="dJ776F14.1; P47; UBX1; UBXD10; UBXN2C"
                     /inference="alignment:Splign:2.1.0"
     exon            811..975
                     /gene="NSFL1C"
                     /gene_synonym="dJ776F14.1; P47; UBX1; UBXD10; UBXN2C"
                     /inference="alignment:Splign:2.1.0"
     exon            976..2721
                     /gene="NSFL1C"
                     /gene_synonym="dJ776F14.1; P47; UBX1; UBXD10; UBXN2C"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1357..1362
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NSFL1C"
                     /gene_synonym="dJ776F14.1; P47; UBX1; UBXD10; UBXN2C"
                     /note="hexamer: AATAAA"
     polyA_site      1376
                     /gene="NSFL1C"
                     /gene_synonym="dJ776F14.1; P47; UBX1; UBXD10; UBXN2C"
                     /note="major polyA site"
     regulatory      2699..2704
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NSFL1C"
                     /gene_synonym="dJ776F14.1; P47; UBX1; UBXD10; UBXN2C"
                     /note="hexamer: ATTAAA"
     polyA_site      2721
                     /gene="NSFL1C"
                     /gene_synonym="dJ776F14.1; P47; UBX1; UBXD10; UBXN2C"
ORIGIN      
        1 gctttggaag gcgcacgggg cgaagatggc ggcggagcga caggaggcgc tgagggagtt
       61 cgtggcggtg acgggcgccg aggaggaccg ggcccgcttc tttctcgagt cggccggctg
      121 ggacttgcag atcgcgctag cgagctttta tgaggacgga ggggatgaag acattgtgac
      181 catttcgcag gcaaccccca gttcagtgtc cagaggcaca gcccccagtg ataatagagt
      241 gacatccttc agagacctca ttcatgacca agatgaagat gaggaggaag aggaaggcca
      301 gaggttttat gctgggggct cagagagaag tggacagcag attgttggcc ctcccaggaa
      361 gaaaagtccc aacgagctgg tggatgatct ctttaaaggt gccaaagagc atggagctgt
      421 agctgtggag cgagtgacca agagccctgg agagaccagt aaaccgagac catttgcagg
      481 aggtggctac cgccttgggg cagcaccaga ggaagagtct gcctatgtgg caggagaaaa
      541 gaggcagcat tccagccaag atgttcatgt agtattgaaa ctctggaaga gtggattcag
      601 cctggataat ggagaactca gaagctacca agacccatcc aatgcccagt ttctggagtc
      661 tatccgcaga ggggaggtgc cagcagagct tcggaggcta gctcacggtg gacaggtgaa
      721 cttggatatg gaggaccatc gggacgagga ctttgtgaag cccaaaggag ccttcaaagc
      781 cttcactggc gagggtcaga aactgggcag cactgccccc caggtgttga gtaccagctc
      841 tccagcccaa caggcagaaa atgaagccaa agccagctct tccatcttaa tcgacgaatc
      901 agagcctacc acaaacatcc aaattcggct tgcagacggc gggaggctgg tgcagaaatt
      961 taaccacagc cacaggatca gcgacatccg actcttcatc gtggatgccc ggccagccat
     1021 ggctgccacc agctttatcc tcatgactac tttcccgaac aaagagctgg ctgatgagag
     1081 ccagaccctg aaggaagcca acctgctcaa tgctgtcatc gtgcagcggt taacataacc
     1141 gcccagccag ctgcctggcc tccctcctgt gtttcccatg gccagtggcc atgccccatg
     1201 gggatcgccc ctcctgcccc cttgtgcaca cccagcagtc cagtgcaacg tctcctccat
     1261 agctctgggt tcttagatct tggttggacg tttgttttct ccttagttgc atttcctggg
     1321 tttttgtgat gatcaatgga ctttaatgaa aaaaaaaata aaaacaacca aaaaaattga
     1381 aggaatatca ccagcatgtt gtacggaaac tctcccactg aagcaggctt taattgcttt
     1441 aaaattatat ttatcttggg gcctgtggga ggaaacttcc ttccatcttc tctgcataaa
     1501 aacttgtggc acacaatgct tattcactag tgtgtcccac ccgccagccc cacagatgac
     1561 tggaggaagg aggggaaatg tgtagaaaga ggcttcgcca ccacttgttc ccacgagaat
     1621 atgtcacttg cccagataaa actgggcggc agccagagtt ccctgaagtg ggaagtcaga
     1681 gctccatgca cacagtgtct tcagaaggtg aaaataaata tttccctgtg ctccttttac
     1741 tcaacccctg gggtatctaa tcttgccagg tcttggccag ttgagattct gttccacctg
     1801 cctgcctggc cctttcctcc attaccatcc agactgctcg cctcctgggg attctcaggg
     1861 gctccattat ggcttgattt actccacgtg cagaagtctt gagtggacct aggaggtagg
     1921 tgggatattt tttttcacta ggatacagct catgccaacc catcctaagt gagttcagaa
     1981 tcagggtatc ttgccctata agataaacag tcaaaatgcc accgagctgt tcactagtga
     2041 tgtgtggcaa atcaaatcaa ctgttgaaga aggggtgagt tttctgtgct acaagcacct
     2101 gtcactgttg gtacttgcag gaggcttctg ctgggtatgt tttggaagtg agtgtcacta
     2161 cttggctttg cttagcaggt tctgcttcac acttgttctt tgacctgctg acttgtgact
     2221 tgcagaaaca taggcagtag tcctagcctg gtaaagaccc tccaccaccc ctataagttt
     2281 gattgctatg caggtttggg agaggaggcc tattgggctc ttggatggaa ccctttcccg
     2341 tattaaacaa accagagaca gaatcagtgc tgactcagga tctcctggtt tggaatcgta
     2401 atgtgcctca atcctctttc caagcaggcc tcaccagtct ctttctcttt cctgcttcac
     2461 ccctgcaatg agccaagaac caacactaca tccacctaga actgcagaag ggcttgtggt
     2521 ttcaaccaag acccatcctg agcaagggac ttggcttggt gcttttgatc ccaaagttcc
     2581 cacaccggca gtggcctgct ggggcaatgg catctgtcac ggtgttttct ccagcaggtg
     2641 gagattatgg aacctacata tgggtctgga aaaactgtac actgttgtca ccttgaccat
     2701 taaaaaccag aatgaggaca a
//
