LOCUS       NM_023034              10768 bp    mRNA    linear   PRI 24-OCT-2024
DEFINITION  Homo sapiens nuclear receptor binding SET domain protein 3 (NSD3),
            transcript variant long, mRNA.
ACCESSION   NM_023034
VERSION     NM_023034.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10768)
  AUTHORS   Zhou,Y., Peng,X., Fang,C., Peng,X., Tang,J., Wang,Z., Long,Y.,
            Chen,J., Peng,Y., Zhang,Z., Zhou,Y., Tang,J., Liao,J., Xiao,D.,
            Tao,Y., Shi,Y. and Liu,S.
  TITLE     Histones Methyltransferase NSD3 Inhibits Lung Adenocarcinoma
            Glycolysis Through Interacting with PPP1CB to Decrease STAT3
            Signaling Pathway
  JOURNAL   Adv Sci (Weinh) 11 (38), e2400381 (2024)
   PUBMED   39119928
  REMARK    GeneRIF: Histones Methyltransferase NSD3 Inhibits Lung
            Adenocarcinoma Glycolysis Through Interacting with PPP1CB to
            Decrease STAT3 Signaling Pathway.
REFERENCE   2  (bases 1 to 10768)
  AUTHORS   Topchu,I., Pangeni,R.P., Bychkov,I., Miller,S.A., Izumchenko,E.,
            Yu,J., Golemis,E., Karanicolas,J. and Boumber,Y.
  TITLE     The role of NSD1, NSD2, and NSD3 histone methyltransferases in
            solid tumors
  JOURNAL   Cell Mol Life Sci 79 (6), 285 (2022)
   PUBMED   35532818
  REMARK    GeneRIF: The role of NSD1, NSD2, and NSD3 histone
            methyltransferases in solid tumors.
            Review article
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 10768)
  AUTHORS   Sun,Y., Xie,J., Cai,S., Wang,Q., Feng,Z., Li,Y., Lu,J.J., Chen,W.
            and Ye,Z.
  TITLE     Elevated expression of nuclear receptor-binding SET domain 3
            promotes pancreatic cancer cell growth
  JOURNAL   Cell Death Dis 12 (10), 913 (2021)
   PUBMED   34615858
  REMARK    GeneRIF: Elevated expression of nuclear receptor-binding SET domain
            3 promotes pancreatic cancer cell growth.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 10768)
  AUTHORS   Zhang,M., Yang,Y., Zhou,M., Dong,A., Yan,X., Loppnau,P., Min,J. and
            Liu,Y.
  TITLE     Histone and DNA binding ability studies of the NSD subfamily of
            PWWP domains
  JOURNAL   Biochem Biophys Res Commun 569, 199-206 (2021)
   PUBMED   34271259
  REMARK    GeneRIF: Histone and DNA binding ability studies of the NSD
            subfamily of PWWP domains.
REFERENCE   5  (bases 1 to 10768)
  AUTHORS   Peng,X., Peng,Q. and Zhong,L.
  TITLE     Targeting H3K36 methyltransferases NSDs: a promising strategy for
            tumor targeted therapy
  JOURNAL   Signal Transduct Target Ther 6 (1), 220 (2021)
   PUBMED   34083510
  REMARK    GeneRIF: Targeting H3K36 methyltransferases NSDs: a promising
            strategy for tumor targeted therapy.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 10768)
  AUTHORS   Tonon,G., Wong,K.K., Maulik,G., Brennan,C., Feng,B., Zhang,Y.,
            Khatry,D.B., Protopopov,A., You,M.J., Aguirre,A.J., Martin,E.S.,
            Yang,Z., Ji,H., Chin,L. and Depinho,R.A.
  TITLE     High-resolution genomic profiles of human lung cancer
  JOURNAL   Proc Natl Acad Sci U S A 102 (27), 9625-9630 (2005)
   PUBMED   15983384
REFERENCE   7  (bases 1 to 10768)
  AUTHORS   Rosati,R., La Starza,R., Veronese,A., Aventin,A., Schwienbacher,C.,
            Vallespi,T., Negrini,M., Martelli,M.F. and Mecucci,C.
  TITLE     NUP98 is fused to the NSD3 gene in acute myeloid leukemia
            associated with t(8;11)(p11.2;p15)
  JOURNAL   Blood 99 (10), 3857-3860 (2002)
   PUBMED   11986249
  REMARK    Erratum:[Blood 2002 Aug 15;100(4):1132]
REFERENCE   8  (bases 1 to 10768)
  AUTHORS   Stec,I., van Ommen,G.J. and den Dunnen,J.T.
  TITLE     WHSC1L1, on human chromosome 8p11.2, closely resembles WHSC1 and
            maps to a duplicated region shared with 4p16.3
  JOURNAL   Genomics 76 (1-3), 5-8 (2001)
   PUBMED   11549311
REFERENCE   9  (bases 1 to 10768)
  AUTHORS   Angrand,P.O., Apiou,F., Stewart,A.F., Dutrillaux,B., Losson,R. and
            Chambon,P.
  TITLE     NSD3, a new SET domain-containing gene, maps to 8p12 and is
            amplified in human breast cancer cell lines
  JOURNAL   Genomics 74 (1), 79-88 (2001)
   PUBMED   11374904
REFERENCE   10 (bases 1 to 10768)
  AUTHORS   Stec,I., Nagl,S.B., van Ommen,G.J. and den Dunnen,J.T.
  TITLE     The PWWP domain: a potential protein-protein interaction domain in
            nuclear proteins influencing differentiation?
  JOURNAL   FEBS Lett 473 (1), 1-5 (2000)
   PUBMED   10802047
  REMARK    Review article
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF332469.1 and AC087362.13.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_023034.1.
            
            Summary: This gene is related to the Wolf-Hirschhorn syndrome
            candidate-1 gene and encodes a protein with PWWP
            (proline-tryptophan-tryptophan-proline) domains. This protein
            methylates histone H3 at lysine residues 4 and 27, which represses
            gene transcription. Two alternatively spliced variants have been
            described. [provided by RefSeq, May 2015].
            
            Transcript Variant: This variant (long) contains an alternative 3'
            end sequence. The longer protein encoded has a different carboxy
            terminus than the protein encoded by variant short.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660809.23424.1, AF332469.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000317025.13/ ENSP00000313983.7
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-5430              AF332469.1         2-5431
            5431-10768          AC087362.13        29703-35040         c
FEATURES             Location/Qualifiers
     source          1..10768
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="8"
                     /map="8p11.23"
     gene            1..10768
                     /gene="NSD3"
                     /gene_synonym="KMT3F; KMT3G; pp14328; WHISTLE; WHSC1L1"
                     /note="nuclear receptor binding SET domain protein 3"
                     /db_xref="GeneID:54904"
                     /db_xref="HGNC:HGNC:12767"
                     /db_xref="MIM:607083"
     exon            1..473
                     /gene="NSD3"
                     /gene_synonym="KMT3F; KMT3G; pp14328; WHISTLE; WHSC1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            474..1192
                     /gene="NSD3"
                     /gene_synonym="KMT3F; KMT3G; pp14328; WHISTLE; WHSC1L1"
                     /inference="alignment:Splign:2.1.0"
     CDS             518..4831
                     /gene="NSD3"
                     /gene_synonym="KMT3F; KMT3G; pp14328; WHISTLE; WHSC1L1"
                     /EC_number="2.1.1.370"
                     /EC_number="2.1.1.371"
                     /note="chromatin modulator; isoform long is encoded by
                     transcript variant long; histone-lysine
                     N-methyltransferase NSD3; Wolf-Hirschhorn syndrome
                     candidate 1-like 1; protein whistle; nuclear SET
                     domain-containing protein 3"
                     /codon_start=1
                     /product="histone-lysine N-methyltransferase NSD3 isoform
                     long"
                     /protein_id="NP_075447.1"
                     /db_xref="CCDS:CCDS43729.1"
                     /db_xref="GeneID:54904"
                     /db_xref="HGNC:HGNC:12767"
                     /db_xref="MIM:607083"
                     /translation="MDFSFSFMQGIMGNTIQQPPQLIDSANIRQEDAFDNNSDIAEDG
                     GQTPYEATLQQGFQYPATTEDLPPLTNGYPSSISVYETQTKYQSYNQYPNGSANGFGA
                     VRNFSPTDYYHSEIPNTRPHEILEKPSPPQPPPPPSVPQTVIPKKTGSPEIKLKITKT
                     IQNGRELFESSLCGDLLNEVQASEHTKSKHESRKEKRKKSNKHDSSRSEERKSHKIPK
                     LEPEEQNRPNERVDTVSEKPREEPVLKEEAPVQPILSSVPTTEVSTGVKFQVGDLVWS
                     KVGTYPWWPCMVSSDPQLEVHTKINTRGAREYHVQFFSNQPERAWVHEKRVREYKGHK
                     QYEELLAEATKQASNHSEKQKIRKPRPQRERAQWDIGIAHAEKALKMTREERIEQYTF
                     IYIDKQPEEALSQAKKSVASKTEVKKTRRPRSVLNTQPEQTNAGEVASSLSSTEIRRH
                     SQRRHTSAEEEEPPPVKIAWKTAAARKSLPASITMHKGSLDLQKCNMSPVVKIEQVFA
                     LQNATGDGKFIDQFVYSTKGIGNKTEISVRGQDRLIISTPNQRNEKPTQSVSSPEATS
                     GSTGSVEKKQQRRSIRTRSESEKSTEVVPKKKIKKEQVETVPQATVKTGLQKGASEIS
                     DSCKPLKKRSRASTDVEMTSSAYRDTSDSDSRGLSDLQVGFGKQVDSPSATADADVSD
                     VQSMDSSLSRRGTGMSKKDTVCQICESSGDSLIPCEGECCKHFHLECLGLASLPDSKF
                     ICMECKTGQHPCFSCKVSGKDVKRCSVGACGKFYHEACVRKFPTAIFESKGFRCPQHC
                     CSACSMEKDIHKASKGRMMRCLRCPVAYHSGDACIAAGSMLVSSYILICSNHSKRSSN
                     SSAVNVGFCFVCARGLIVQDHSDPMFSSYAYKSHYLLNESNRAELMKLPMIPSSSASK
                     KKCEKGGRLLCCESCPASFHPECLSIEMPEGCWNCNDCKAGKKLHYKQIVWVKLGNYR
                     WWPAEICNPRSVPLNIQGLKHDLGDFPVFFFGSHDYYWVHQGRVFPYVEGDKSFAEGQ
                     TSINKTFKKALEEAAKRFQELKAQRESKEALEIEKNSRKPPPYKHIKANKVIGKVQIQ
                     VADLSEIPRCNCKPADENPCGLESECLNRMLQYECHPQVCPAGDRCQNQCFTKRLYPD
                     AEIIKTERRGWGLRTKRSIKKGEFVNEYVGELIDEEECRLRIKRAHENSVTNFYMLTV
                     TKDRIIDAGPKGNYSRFMNHSCNPNCETQKWTVNGDVRVGLFALCDIPAGMELTFNYN
                     LDCLGNGRTECHCGADNCSGFLGVRPKSACASTNEEKAKNAKLKQKRRKIKTEPKQMH
                     EDYCFQCGDGGELVMCDKKDCPKAYHLLCLNLTQPPYGKWECPWHQCDECSSAAVSFC
                     EFCPHSFCKDHEKGALVPSALEGRLCCSEHDPMAPVSPEYWSKIKCKWESQDHGEEVK
                     E"
     exon            1193..1264
                     /gene="NSD3"
                     /gene_synonym="KMT3F; KMT3G; pp14328; WHISTLE; WHSC1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            1265..1427
                     /gene="NSD3"
                     /gene_synonym="KMT3F; KMT3G; pp14328; WHISTLE; WHSC1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            1428..1582
                     /gene="NSD3"
                     /gene_synonym="KMT3F; KMT3G; pp14328; WHISTLE; WHSC1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            1583..2098
                     /gene="NSD3"
                     /gene_synonym="KMT3F; KMT3G; pp14328; WHISTLE; WHSC1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            2099..2225
                     /gene="NSD3"
                     /gene_synonym="KMT3F; KMT3G; pp14328; WHISTLE; WHSC1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            2226..2326
                     /gene="NSD3"
                     /gene_synonym="KMT3F; KMT3G; pp14328; WHISTLE; WHSC1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            2327..2372
                     /gene="NSD3"
                     /gene_synonym="KMT3F; KMT3G; pp14328; WHISTLE; WHSC1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            2373..2503
                     /gene="NSD3"
                     /gene_synonym="KMT3F; KMT3G; pp14328; WHISTLE; WHSC1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            2504..2632
                     /gene="NSD3"
                     /gene_synonym="KMT3F; KMT3G; pp14328; WHISTLE; WHSC1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            2633..2759
                     /gene="NSD3"
                     /gene_synonym="KMT3F; KMT3G; pp14328; WHISTLE; WHSC1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            2760..2957
                     /gene="NSD3"
                     /gene_synonym="KMT3F; KMT3G; pp14328; WHISTLE; WHSC1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            2958..3128
                     /gene="NSD3"
                     /gene_synonym="KMT3F; KMT3G; pp14328; WHISTLE; WHSC1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            3129..3275
                     /gene="NSD3"
                     /gene_synonym="KMT3F; KMT3G; pp14328; WHISTLE; WHSC1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            3276..3432
                     /gene="NSD3"
                     /gene_synonym="KMT3F; KMT3G; pp14328; WHISTLE; WHSC1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            3433..3635
                     /gene="NSD3"
                     /gene_synonym="KMT3F; KMT3G; pp14328; WHISTLE; WHSC1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            3636..3748
                     /gene="NSD3"
                     /gene_synonym="KMT3F; KMT3G; pp14328; WHISTLE; WHSC1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            3749..4018
                     /gene="NSD3"
                     /gene_synonym="KMT3F; KMT3G; pp14328; WHISTLE; WHSC1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            4019..4135
                     /gene="NSD3"
                     /gene_synonym="KMT3F; KMT3G; pp14328; WHISTLE; WHSC1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            4136..4277
                     /gene="NSD3"
                     /gene_synonym="KMT3F; KMT3G; pp14328; WHISTLE; WHSC1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            4278..4384
                     /gene="NSD3"
                     /gene_synonym="KMT3F; KMT3G; pp14328; WHISTLE; WHSC1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            4385..4589
                     /gene="NSD3"
                     /gene_synonym="KMT3F; KMT3G; pp14328; WHISTLE; WHSC1L1"
                     /inference="alignment:Splign:2.1.0"
     exon            4590..10768
                     /gene="NSD3"
                     /gene_synonym="KMT3F; KMT3G; pp14328; WHISTLE; WHSC1L1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      10744..10749
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NSD3"
                     /gene_synonym="KMT3F; KMT3G; pp14328; WHISTLE; WHSC1L1"
                     /note="hexamer: AATAAA"
     polyA_site      10768
                     /gene="NSD3"
                     /gene_synonym="KMT3F; KMT3G; pp14328; WHISTLE; WHSC1L1"
                     /note="major polyA site"
ORIGIN      
        1 ggggctttgt gcgcggcggc ggcgggagag gcggcggcgg cggccagcac ggaggcggag
       61 gccgaggggg ctgtgcacag gtcgccgcgg agaggcgtgc gaattccgag ccgagcgccg
      121 aggaccgtgc tacccaggcc gggctgccag ccgcaggctc ctctctggca gcagcggcgg
      181 cgcggcgacc cccgtccctc ggcctcccct tcccatccca cctcccgagc cttcctcttc
      241 ccgcagcacg cccggcccgg cccggccgtg gccctcctca gtgccggccg ccatggcaga
      301 ggcgtccggc gcggggaaaa tctagcccgg ggatttcatg cggcctagct cggttccgcc
      361 tcctcctcgc gcggccccag cggctgcccg caccccagcc ccactccggg cctccgtgtc
      421 tctcctgtga tcgcactgac acggccgggg ggttagaatg gaacaaactg aaggcccgat
      481 gagagaaagg gaaagttaag gatgctggag cagaacaatg gatttctctt tctctttcat
      541 gcaagggatc atgggaaaca caattcagca accacctcaa ctcattgact ccgccaacat
      601 ccgtcaggag gatgcctttg ataacaacag tgacattgct gaagatggtg gccagacacc
      661 atatgaagct actttgcagc aaggctttca gtacccagct acaacagaag atcttcctcc
      721 actcacaaat gggtatccat catcaatcag tgtgtatgaa actcaaacca aataccagtc
      781 atataatcag tatcctaatg ggtcagccaa tggctttggt gcagttagaa actttagccc
      841 cactgactat tatcattcag aaattccaaa cacaagacca catgaaattc tggaaaaacc
      901 ttcccctcca cagccaccac ctcctccttc ggtaccacaa actgtgattc caaagaagac
      961 tggctcacct gaaattaaac taaaaataac caaaactatc cagaatggca gggaattgtt
     1021 tgagtcttcc ctttgtggag accttttaaa tgaagtacag gcaagtgagc acacgaaatc
     1081 aaagcatgaa agcagaaaag aaaagaggaa aaaaagcaac aagcatgact catcaagatc
     1141 tgaagagcgc aagtcacaca aaatccccaa attagaacca gaggaacaaa atagaccaaa
     1201 tgagagggtt gacactgtat cagaaaaacc aagggaagaa ccagtactaa aagaggaagc
     1261 cccagttcag ccaatactat cttctgttcc aacaacggaa gtgtccactg gtgttaagtt
     1321 tcaggttggc gatcttgtgt ggtccaaggt gggaacctat ccttggtggc cttgtatggt
     1381 ttcaagtgat ccccagcttg aggttcatac taaaattaac acaagaggtg cccgagaata
     1441 tcatgtccag ttttttagca accagccaga gagggcgtgg gttcatgaaa aacgggtacg
     1501 agagtataaa ggtcataaac agtatgaaga attactggct gaggcaacca aacaagccag
     1561 caatcactct gagaaacaaa agattcggaa accccgacct cagagagaac gtgctcagtg
     1621 ggatattggc attgcccatg cagagaaagc attgaaaatg actcgagaag aaagaataga
     1681 acagtatact tttatttaca ttgataaaca gcctgaagag gctttatccc aagcaaaaaa
     1741 gagtgttgcc tccaaaaccg aagttaaaaa aacccgacga ccaagatctg tgctgaatac
     1801 tcagccagaa cagaccaatg caggggaggt ggcctcctca ctctcaagta ctgaaattcg
     1861 gagacatagc cagaggcggc acacaagtgc ggaagaggaa gagccaccgc ctgttaaaat
     1921 agcctggaaa actgcggcag caaggaaatc cttaccagct tccattacga tgcacaaagg
     1981 gagcctggat ttgcagaagt gtaacatgtc tccagttgtg aaaattgaac aagtgtttgc
     2041 tcttcagaat gctacagggg atgggaaatt tatcgatcaa tttgtttatt caacaaaggg
     2101 aattggtaac aaaacagaaa taagtgtcag ggggcaagac aggcttataa tttctacacc
     2161 aaaccagaga aatgaaaagc caacgcagag tgtatcatct cctgaagcaa catctggttc
     2221 tacaggctca gtagaaaaga agcaacagag aagatcaatt agaactcgtt ctgaatcaga
     2281 gaaatccact gaggttgtgc caaagaagaa gatcaaaaag gagcaggttg aaacagttcc
     2341 tcaggctaca gtgaagactg gattacagaa aggtgccagc gagatttcag attcctgtaa
     2401 acctctaaag aaaaggagtc gcgcctcaac tgatgtagaa atgactagtt cagcatacag
     2461 agacacatct gactccgatt ctagaggact gagtgacctg caggtaggct ttggaaagca
     2521 agtagatagc ccttcagcta ctgcagatgc agacgtttct gatgtgcagt ccatggattc
     2581 aagtttgtcg agaagaggca ctggaatgag taagaaggac actgtatgtc agatttgtga
     2641 aagctctggt gactctctga ttccttgtga gggagagtgc tgcaaacact ttcacctgga
     2701 gtgcctggga ttggcatcac ttcctgatag caagttcatc tgcatggaat gtaaaactgg
     2761 gcagcaccca tgtttttcgt gtaaagtgtc tggtaaagat gtgaagcgtt gttctgttgg
     2821 tgcttgtggg aaattttatc atgaagcctg tgtccgcaaa ttccccactg ccatctttga
     2881 atcaaaagga ttccgctgtc ctcagcactg ctgctctgcc tgctctatgg agaaagatat
     2941 ccacaaagca agtaaaggcc gcatgatgag atgtttaaga tgtccagttg cctatcactc
     3001 tggagatgct tgcattgcgg ccggaagcat gttagtatcc tcctacattc tcatctgtag
     3061 taatcattcc aaacggagca gtaattcttc tgctgtaaat gtaggctttt gtttcgtttg
     3121 tgccagaggg ctgatagttc aggaccattc agaccccatg ttcagttcat atgcctataa
     3181 gtcccactac ctactgaatg aatcaaatcg tgctgagttg atgaaattac ctatgattcc
     3241 ttcttcgtca gcttccaaaa agaaatgtga gaaaggtgga agattgctct gctgtgaatc
     3301 gtgcccagct tccttccacc cggaatgcct aagcatagaa atgccagaag gctgctggaa
     3361 ttgtaatgac tgtaaagctg gcaagaaact acattacaag cagattgttt gggtcaaatt
     3421 gggaaattac agatggtggc cagcagagat ctgcaacccc aggtctgtgc cactgaacat
     3481 ccagggcctt aaacatgact tgggggactt ccctgtattc ttctttggtt ctcatgacta
     3541 ctactgggta caccagggca gagtgttccc ttatgttgaa ggagacaaaa gctttgctga
     3601 agggcagact agtattaaca agaccttcaa aaaggcactg gaagaagctg caaaacgttt
     3661 ccaggaattg aaagcacaaa gagaaagtaa agaagcccta gagattgaaa aaaactcaag
     3721 aaaaccccct ccctacaaac acatcaaagc taacaaagta ataggaaagg tgcagatcca
     3781 ggttgctgac ctgtcagaga ttccccgctg taactgcaag ccagctgatg aaaacccttg
     3841 tggcttggaa tcggagtgcc tgaacagaat gttgcagtat gaatgccacc cgcaggtgtg
     3901 cccagctgga gatcgttgtc agaaccagtg ctttacaaag agactatacc ctgatgcaga
     3961 gatcatcaaa acggagcgga gaggctgggg cctcaggacc aaaaggagca ttaagaaggg
     4021 tgaatttgta aatgaatacg tcggtgaatt aattgatgaa gaagaatgca gattgcgaat
     4081 caagcgagcc cacgagaaca gtgtaactaa tttttatatg ttaactgtta ccaaggaccg
     4141 tataattgat gccggcccaa aaggaaatta ttctcgcttc atgaaccaca gttgtaatcc
     4201 caactgtgaa acacaaaagt ggacagtgaa tggagatgtt cgagtgggac tatttgctct
     4261 ctgtgatatt cctgcaggga tggagttaac atttaattat aacctagatt gtctgggcaa
     4321 cggcagaacg gagtgccact gtggagcaga taactgcagt ggttttctag gagtgcggcc
     4381 aaagtcggca tgtgcgtcaa caaatgaaga gaaggcaaaa aatgctaagt taaaacagaa
     4441 gagacgaaag atcaaaacag aaccaaagca gatgcatgaa gattactgtt ttcaatgtgg
     4501 agatggtgga gagctggtca tgtgtgacaa aaaagactgt cccaaagcat accacctcct
     4561 atgccttaac ctgactcagc caccatatgg aaagtgggag tgtccgtggc atcagtgcga
     4621 tgagtgcagc agtgcagctg tttccttctg tgaattctgt ccacattcat tttgtaaaga
     4681 tcatgaaaag ggggccctgg ttccctctgc actggaaggc cgcctctgct gctcggaaca
     4741 tgaccccatg gctcctgtgt caccagaata ctggagcaag ataaaatgta aatgggaatc
     4801 acaagatcat ggagaagaag taaaagaata aatgtgtggt gtcccctcct ttctatttaa
     4861 gtgaaaaaag caaatagatc atgcatttaa aaagaagaga ctgctacagt gcatacagcc
     4921 tttgccatcg gaactgcctt attaaagcaa aaatgggaaa ccagttcatg caggcagaag
     4981 cagttggtgg tgtctggttt ttgtttgatt tggttggttt gggattcttt tgtggagggt
     5041 taaattccct tggtcttttc ttgcctttta ttgtgcttca gtgccattgc agcttgaaaa
     5101 agaaatgttt ttgctgttaa aataagaaca aagagaaaag taagttttgt taatgagata
     5161 aatttaaagt ctaagatgtg ttccttggtt gtataaagca aaagtagcca tcattccttt
     5221 atttattttc atttttagga atttcaagaa gtgtagttca atagtctaat caagtgtgtg
     5281 tgtgttttaa gtaggaatct gagaaagccc tctaggaaag ggtatgataa gctttatata
     5341 cctctttact gagcagtagg taggctcact tctctttccc ttcaaaatgc ttttcatagg
     5401 cttagagaag ggctctatgg aagtattaaa tctggcctct tgaaaacaat gcctcggtgg
     5461 attttacctc tgtggtctta aacaaggtgg gctttgtact atggtaactt tacctagaag
     5521 ggtatatggt gtgtttcctt ggtggaagga aaaaaaggaa tggatgtgct cactgatttt
     5581 taaaacgttt tccattgaca tggatggttt gggttttgga tgaagtgcgt ttctgtggat
     5641 atgaagcata gttggaatgg tcactcctat tttcctgaca ctgattaagg cagctaaagg
     5701 gggatgaagg cataaaccaa aatttggatc atataagcac cattttctgt ccaattttat
     5761 tgtagaccct ggtcttaatt ggagagaatt acaggcattt tattttttgt ctctgttttc
     5821 ctctcctact caaacatttc ttttgtcatc tgaccattca gtcatgtttc ctggtaaaac
     5881 cacacaagtg agtttgaatt gtatttgaat agatcttttt ggttgagttc ctaatttggg
     5941 gggtgaaagt agtaggggca tccccaggtt acatgtaaag aatttctatt attatccagt
     6001 acttaagatt gctcccattg agtaagggga agaacttcta cttttacagg gtgcataaga
     6061 gccacacaaa cacacttcat cttaggaagc acttggagaa aactcccaag aaagtcagtg
     6121 ttagcaaaaa tggttgatgg tgatgcttga ctcttataga tgcgagtata ccaagatgtc
     6181 tgctcaccaa actcacttgc ttttaatgct taggaatatg tatattccag tattccttgt
     6241 atgaaaataa ttagtaatgt cagacattgt tgtaacactg tactaagtag aagtctaggg
     6301 ttagcttgaa tgaatgttaa ctttctctgt gaattttgac tgcttacaat tgctggtacc
     6361 tggtagcatt tattttccct caagtaccta gtaaccttat gaaggtaggg agggtagatg
     6421 ctctaccaca ttccttcagc tgtattgagt gttccatttg cttatcaatt tagagctctt
     6481 ctatggaaag agggtgaaaa tctgttttgt gcctcttttt tttgtatgta aatgtgcaat
     6541 tatactggat tttctcttgt aaaaaacact acaattcttt tactgaagct cctaaactgc
     6601 catttgcctg actccagcaa ttaattctgc ggtgactcat tgggcttcca gtacttctgt
     6661 tgattagata tggtcccaaa agaaagagca aaatggaaaa tgcgatccat gtcaccaaat
     6721 ataattgcta caagtaacat gaaatacagc tcaccaaaac actaaacttt gctcttgagc
     6781 aattatatag tttgatgtcc ttttaaaaaa taagaaaagc taggttttat catactaaaa
     6841 ttttattttg tatatgatgt ttggtttttt aaacttacta acataaggat ttcctttaat
     6901 attccaaata cagttcttga aacagtatcc aacatgaaat cttatctcct tgcttttaga
     6961 cttagtgcta tttactatct aaagactttt ctatggtaag ccagggtaca tcctatatac
     7021 tacagatacc ttaggtttca gtatttttat gcagcttctt cattgtgtca cattttgttt
     7081 tcatatctgt aacagagctc tgctgaaatt aaaaaaaaat tatgattaat aggattgttt
     7141 caccatttca gaaatcagag tagaaaacaa aatagctgcc aggtgcagtg gctcacacct
     7201 gtaatcccag cactttggga ggccgaggtg ggcagatcat gaggtcagga gtttgagacc
     7261 agcctgacca acatggtgaa accctgtctc tgctaaaaat acaaaaagga gccgggcatg
     7321 gtggcacata cctgtagtcc cagtttctca ggaggcagag gttgcagtga gccaagatca
     7381 catcactgca ctccagcctg ggtgacagag cgagactcca tctcaaaaaa aaaaggtaac
     7441 catagttctg aagtctttgg ccatgaatgt tctttaatgg tctaaaaagc ggttttagtc
     7501 atgctggtgc caaggagttc tcctcacacc tccatagcgt caatgttgac ctatttgata
     7561 tatttttaag ttgcagatta tagatgcaga aagagaaaat tgtcctttat gtgtgcatga
     7621 gtatacatgc actgtgcctg tgatacacca atgaaatatt ccaaaagaca ttgctcactc
     7681 atagtctgaa gttggtattt gttaactttt aaatctatat gtggtcagag tgtaagtggc
     7741 tggtttttat tcttgtccaa caatgaaata acttaaagca gcatgggcta atggtggtat
     7801 tttttttaac cacatgttaa tttgttgaag gaagaagtga acataagatt tttgataatc
     7861 agcctaaagc ttcagaaact ggatcagtaa gagctaaaga tgaaatcctg ggaaactttc
     7921 tcaggcactg tgcaggggag ggaggggctg gaccgccact gctgcgaggg aagaatcaga
     7981 agagctgcct gtggctggct gagaagggca aggtggatgg ctcttcattc ctggaaggct
     8041 gaagcagcca gaggcagtga ttcacctctt taatgtctaa tcagggagag atacaggagt
     8101 gaggtgggtt ttattttcat ttttgtttta acaagttccc actcttatct taattctcaa
     8161 aaatgatctg gcctcatcta tagaactggc tgagttgggg aatcatttaa aatttgggat
     8221 tttctgtaca agtcaagaac atatgtgaaa gctaaaaaag taatggaggc agggggactg
     8281 ctcccagctc ccttctgcca tttacacaaa atatttgaaa acatgtctgt aaataacgtt
     8341 gtggaactga gatattgagt atctaatcat gtaaaggacc ctcagatggg acaggctatt
     8401 ggacaacttt cctggttcat gaagtggaat ttcctttttg agtagtttct tgcatggtgt
     8461 attccaatag gtcaaaccca gacctactgt ttgcaaacag gccatctgta gagaatacaa
     8521 tctctggact gcccaggact tgttgcttgt ctttcatttc attttaaata caaagctctt
     8581 cctttagcat tcaccttgaa gtcaaggtgt tggctgccac atgccatgtc ctcttaacaa
     8641 gataagaaaa cagtggctat ggagccctca cagctgggaa gctgacattc tatacttcca
     8701 tatgagaaaa aggacattgg aaagttgctt tatttctggg cagcgtagac taaagttact
     8761 tttcacaagg ctgatgcatc cagcctcccc cagttgtcca agttggtgca gacatgaatt
     8821 gatgaatgga aacactttcc tttggcctag ggccacctag aattggtctc tggttatttg
     8881 taaaacaaaa agtagaactt gcttccattt tcagatggac tagggaatac tataattaga
     8941 cagctttgca atgaattaac aaatactgaa aagttggaaa tgtgtatata ctgaaatctc
     9001 accaactttt acctggggtg gggtaccagg agaaagggaa ccccctttct cctggtaaag
     9061 ggtaaggggg gggataatgt ttaccacagg tacgaaatag tcactttaac attgagacct
     9121 ctgcctcatt gaattcaggt tttttaagta cttgaaactc ttcagattct ccttatttta
     9181 gtttcttttt acatttatga agtagaaagc attgttttgt aaactgtttt gaaaataaat
     9241 agcctagtct cttatcctct ttagcgtgga ttaaaggtga agttctgcaa atgggagagt
     9301 gttcacagta gatagctcag attgattgaa cacatttgag gaagagactc ctgcatgaga
     9361 taccagcatt tttacaaata ctttttatgt acattcttta ttttgtcatt ttgtcaaccc
     9421 tctccccaag cacatcttct ttccttttac tatgtctatg tagggaaaaa aacaaaacaa
     9481 aaaattgcac ttacgttaca ctcccaaaat gtgggtaatc cgtgtctttc aaaaaacatt
     9541 tctgtttttt gttttgtttt ggtcagtcca ttgcataagt gacaagtttg ggtgcttgtg
     9601 gcacgtatgt atgaagcggg agggggatga gaattgcctg tccttcagta ggctgtaaaa
     9661 gtaatttaca tgtaagtaaa aagggaaaat agaatagatg ccaaagtcat ttattcagtc
     9721 cttagttttc ttatgtggca ttactgcatc tgctagttag tgagaaagca ccctcagctt
     9781 ttactgctcc cctccctgcc tgccaacaca cttgatgtgt gcaaacagcc ctcaagtatc
     9841 tgtcagatga cctatataag gtattgaata aggtattctt gtcagtttag aaatggactg
     9901 gataaaactt acttggttgt cattatttta tctcatttgt cctgttacat gccctatgtt
     9961 aagataatta tattgccact aataatcaag atgctaaatg agtattacaa ctggctaata
    10021 tcatttttta tatacaaggg tatgtgtata tttggaattg atatgagaaa ctcatttgta
    10081 cccatttgag tgatattgca caacaaacac agatacctac agactccgtt ttcattttct
    10141 cgtgttcttt atgataatga tctttgtaga ttggttattt ctgtacttta tctgtaataa
    10201 actttgtaga tcctgtgaac cattactttg cctaaatcac ttgagacttg agtctttaat
    10261 aacaaagcat caatattcac taaagtcaat ctcttttgag tttctgtgac ttggctagaa
    10321 gctcttgaca ctaagggatt agtgttaatt ttccctgggg gtgttccact agggcattac
    10381 tgtataatga cttgatgttg ccacatagac ttcaagatat ataatatttt gaggattttg
    10441 ttgattggcc tatgttttat tgcatagtgt gaaacgtgta aagcttggtt aacctgtata
    10501 tagatagctt attgttgact agttatagtg tatttagggt tgcctgtaat atttaagctt
    10561 ctttactgat gtgtgtgctg gtaggaacat ataatttttg tacattatat ttactgagat
    10621 gttgcctttt ttattttaca aatactttgg aattccaatg tgttttttgc ttccgtgagg
    10681 attaatttgg aaaggttttt aatgacattc cactgatttc agattttgct tgagattgac
    10741 ttcaataaat tgtcctgtat gttccaaa
//
