LOCUS       NM_001330078            9038 bp    mRNA    linear   PRI 20-JUN-2024
DEFINITION  Homo sapiens neurexin 1 (NRXN1), transcript variant alpha4, mRNA.
ACCESSION   NM_001330078 XM_011533171
VERSION     NM_001330078.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9038)
  AUTHORS   Sebastian,R., Jin,K., Pavon,N., Bansal,R., Potter,A., Song,Y.,
            Babu,J., Gabriel,R., Sun,Y., Aronow,B. and Pak,C.
  TITLE     Schizophrenia-associated NRXN1 deletions induce
            developmental-timing- and cell-type-specific vulnerabilities in
            human brain organoids
  JOURNAL   Nat Commun 14 (1), 3770 (2023)
   PUBMED   37355690
  REMARK    GeneRIF: Schizophrenia-associated NRXN1 deletions induce
            developmental-timing- and cell-type-specific vulnerabilities in
            human brain organoids.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9038)
  AUTHORS   Zhong,Y., An,L., Wang,Y., Yang,L. and Cao,Q.
  TITLE     Functional abnormality in the sensorimotor system attributed to
            NRXN1 variants in boys with attention deficit hyperactivity
            disorder
  JOURNAL   Brain Imaging Behav 16 (3), 967-976 (2022)
   PUBMED   34687402
  REMARK    GeneRIF: Functional abnormality in the sensorimotor system
            attributed to NRXN1 variants in boys with attention deficit
            hyperactivity disorder.
REFERENCE   3  (bases 1 to 9038)
  AUTHORS   Rhoades,R., Henry,B., Prichett,D., Fang,Y. and Teng,S.
  TITLE     Computational Saturation Mutagenesis to Investigate the Effects of
            Neurexin-1 Mutations on AlphaFold Structure
  JOURNAL   Genes (Basel) 13 (5), 789 (2022)
   PUBMED   35627176
  REMARK    GeneRIF: Computational Saturation Mutagenesis to Investigate the
            Effects of Neurexin-1 Mutations on AlphaFold Structure.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9038)
  AUTHORS   Aksu Uzunhan,T. and Ayaz,A.
  TITLE     Homozygous exonic and intragenic NRXN1 deletion presenting as
            either West syndrome or autism spectrum disorder in two siblings
  JOURNAL   Clin Neurol Neurosurg 214, 107141 (2022)
   PUBMED   35101781
  REMARK    GeneRIF: Homozygous exonic and intragenic NRXN1 deletion presenting
            as either West syndrome or autism spectrum disorder in two
            siblings.
REFERENCE   5  (bases 1 to 9038)
  AUTHORS   Yan,Q., Weyn-Vanhentenryck,S.M., Wu,J., Sloan,S.A., Zhang,Y.,
            Chen,K., Wu,J.Q., Barres,B.A. and Zhang,C.
  TITLE     Systematic discovery of regulated and conserved alternative exons
            in the mammalian brain reveals NMD modulating chromatin regulators
  JOURNAL   Proc Natl Acad Sci U S A 112 (11), 3445-3450 (2015)
   PUBMED   25737549
REFERENCE   6  (bases 1 to 9038)
  AUTHORS   Tabuchi,K. and Sudhof,T.C.
  TITLE     Structure and evolution of neurexin genes: insight into the
            mechanism of alternative splicing
  JOURNAL   Genomics 79 (6), 849-859 (2002)
   PUBMED   12036300
REFERENCE   7  (bases 1 to 9038)
  AUTHORS   Rowen,L., Young,J., Birditt,B., Kaur,A., Madan,A., Philipps,D.L.,
            Qin,S., Minx,P., Wilson,R.K., Hood,L. and Graveley,B.R.
  TITLE     Analysis of the human neurexin genes: alternative splicing and the
            generation of protein diversity
  JOURNAL   Genomics 79 (4), 587-597 (2002)
   PUBMED   11944992
REFERENCE   8  (bases 1 to 9038)
  AUTHORS   Ichtchenko,K., Hata,Y., Nguyen,T., Ullrich,B., Missler,M.,
            Moomaw,C. and Sudhof,T.C.
  TITLE     Neuroligin 1: a splice site-specific ligand for beta-neurexins
  JOURNAL   Cell 81 (3), 435-443 (1995)
   PUBMED   7736595
REFERENCE   9  (bases 1 to 9038)
  AUTHORS   Ullrich,B., Ushkaryov,Y.A. and Sudhof,T.C.
  TITLE     Cartography of neurexins: more than 1000 isoforms generated by
            alternative splicing and expressed in distinct subsets of neurons
  JOURNAL   Neuron 14 (3), 497-507 (1995)
   PUBMED   7695896
REFERENCE   10 (bases 1 to 9038)
  AUTHORS   Ushkaryov,Y.A., Petrenko,A.G., Geppert,M. and Sudhof,T.C.
  TITLE     Neurexins: synaptic cell surface proteins related to the
            alpha-latrotoxin receptor and laminin
  JOURNAL   Science 257 (5066), 50-56 (1992)
   PUBMED   1621094
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC007682.3, AC009234.3,
            AC007462.3, AC068725.5, AC069550.4 and AC078994.3.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_001330078.1.
            
            Summary: This gene encodes a single-pass type I membrane protein
            that belongs to the neurexin family. Neurexins are cell-surface
            receptors that bind neuroligins to form Ca(2+)-dependent
            neurexin/neuroligin complexes at synapses in the central nervous
            system. This complex is required for efficient neurotransmission
            and is involved in the formation of synaptic contacts. Three
            members of this gene family have been studied in detail and are
            estimated to generate over 3,000 variants through the use of two
            alternative promoters (alpha and beta) and extensive alternative
            splicing in each family member. Recently, a third promoter (gamma)
            was identified for this gene in the 3' region. Mutations in this
            gene are associated with Pitt-Hopkins-like syndrome-2 and may
            contribute to susceptibility to schizophrenia. [provided by RefSeq,
            Aug 2016].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA1965299, SAMEA1966682
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000401669.7/ ENSP00000385017.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-152               AC007682.3         162922-163073       c
            153-1845            AC007682.3         158443-160135       c
            1846-1863           AC007682.3         56879-56896         c
            1864-1893           AC007682.3         53599-53628         c
            1894-1905           AC007682.3         52810-52821         c
            1906-2207           AC009234.3         35329-35630         c
            2208-2231           AC009234.3         33241-33264         c
            2232-2393           AC009234.3         32037-32198         c
            2394-2832           AC007462.3         127866-128304       c
            2833-3216           AC007462.3         113532-113915       c
            3217-3420           AC007462.3         106506-106709       c
            3421-3447           AC007462.3         103904-103930       c
            3448-3570           AC007462.3         81774-81896         c
            3571-3952           AC007462.3         72612-72993         c
            3953-4143           AC007462.3         71184-71374         c
            4144-4317           AC007462.3         47577-47750         c
            4318-4437           AC007462.3         40721-40840         c
            4438-4619           AC068725.5         115017-115198
            4620-4791           AC069550.4         112425-112596
            4792-4881           AC069550.4         148875-148964
            4882-5201           AC069550.4         150329-150648
            5202-5289           AC078994.3         97384-97471
            5290-9038           AC078994.3         118924-122672
FEATURES             Location/Qualifiers
     source          1..9038
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2p16.3"
     gene            1..9038
                     /gene="NRXN1"
                     /gene_synonym="Hs.22998; PTHSL2; SCZD17"
                     /note="neurexin 1"
                     /db_xref="GeneID:9378"
                     /db_xref="HGNC:HGNC:8008"
                     /db_xref="MIM:600565"
     exon            1..152
                     /gene="NRXN1"
                     /gene_synonym="Hs.22998; PTHSL2; SCZD17"
                     /inference="alignment:Splign:2.1.0"
     exon            153..1845
                     /gene="NRXN1"
                     /gene_synonym="Hs.22998; PTHSL2; SCZD17"
                     /inference="alignment:Splign:2.1.0"
     CDS             1074..5597
                     /gene="NRXN1"
                     /gene_synonym="Hs.22998; PTHSL2; SCZD17"
                     /note="isoform alpha4 precursor is encoded by transcript
                     variant alpha4; neurexin I"
                     /codon_start=1
                     /product="neurexin-1 isoform alpha4 precursor"
                     /protein_id="NP_001317007.1"
                     /db_xref="CCDS:CCDS82451.1"
                     /db_xref="GeneID:9378"
                     /db_xref="HGNC:HGNC:8008"
                     /db_xref="MIM:600565"
                     /translation="MGTALLQRGGCFLLCLSLLLLGCWAELGSGLEFPGAEGQWTRFP
                     KWNACCESEMSFQLKTRSARGLVLYFDDEGFCDFLELILTRGGRLQLSFSIFCAEPAT
                     LLADTPVNDGAWHSVRIRRQFRNTTLFIDQVEAKWVEVKSKRRDMTVFSGLFVGGLPP
                     ELRAAALKLTLASVREREPFKGWIRDVRVNSSQVLPVDSGEVKLDDEPPNSGGGSPCE
                     AGEEGEGGVCLNGGVCSVVDDQAVCDCSRTGFRGKDCSQEDNNVEGLAHLMMGDQGKS
                     KGKEEYIATFKGSEYFCYDLSQNPIQSSSDEITLSFKTLQRNGLMLHTGKSADYVNLA
                     LKNGAVSLVINLGSGAFEALVEPVNGKFNDNAWHDVKVTRNLRQHSGIGHAMVTISVD
                     GILTTTGYTQEDYTMLGSDDFFYVGGSPSTADLPGSPVSNNFMGCLKEVVYKNNDVRL
                     ELSRLAKQGDPKMKIHGVVAFKCENVATLDPITFETPESFISLPKWNAKKTGSISFDF
                     RTTEPNGLILFSHGKPRHQKDAKHPQMIKVDFFAIEMLDGHLYLLLDMGSGTIKIKAL
                     LKKVNDGEWYHVDFQRDGRSGTISVNTLRTPYTAPGESEILDLDDELYLGGLPENKAG
                     LVFPTEVWTALLNYGYVGCIRDLFIDGQSKDIRQMAEVQSTAGVKPSCSKETAKPCLS
                     NPCKNNGMCRDGWNRYVCDCSGTGYLGRSCEREATVLSYDGSMFMKIQLPVVMHTEAE
                     DVSLRFRSQRAYGILMATTSRDSADTLRLELDAGRVKLTVNLDCIRINCNSSKGPETL
                     FAGYNLNDNEWHTVRVVRRGKSLKLTVDDQQAMTGQMAGDHTRLEFHNIETGIITERR
                     YLSSVPSNFIGHLQSLTFNGMAYIDLCKNGDIDYCELNARFGFRNIIADPVTFKTKSS
                     YVALATLQAYTSMHLFFQFKTTSLDGLILYNSGDGNDFIVVELVKGYLHYVFDLGNGA
                     NLIKGSSNKPLNDNQWHNVMISRDTSNLHTVKIDTKITTQITAGARNLDLKSDLYIGG
                     VAKETYKSLPKLVHAKEGFQGCLASVDLNGRLPDLISDALFCNGQIERGCEGPSTTCQ
                     EDSCSNQGVCLQQWDGFSCDCSMTSFSGPLCNDPGTTYIFSKGGGQITYKWPPNDRPS
                     TRADRLAIGFSTVQKEAVLVRVDSSSGLGDYLELHIHQGKIGVKFNVGTDDIAIEESN
                     AIINDGKYHVVRFTRSGGNATLQVDSWPVIERYPAGNNDNERLAIARQRIPYRLGRVV
                     DEWLLDKGRQLTIFNSQATIIIGGKEQGQPFQGQLSGLYYNGLKVLNMAAENDANIAI
                     VGNVRLVGEVPSSMTTESTATAMQSEMSTSIMETTTTLATSTARRGKPPTKEPISQTT
                     DDILVASAECPSDDEDIDPCEPSSGGLANPTRAGGREPYPGSAEVIRESSSTTGMVVG
                     IVAAAALCILILLYAMYKYRNRDEGSYHVDESRNYISNSAQSNGAVVKEKQPSSAKSS
                     NKNKKNKDKEYYV"
     exon            1846..1863
                     /gene="NRXN1"
                     /gene_synonym="Hs.22998; PTHSL2; SCZD17"
                     /inference="alignment:Splign:2.1.0"
     exon            1864..1893
                     /gene="NRXN1"
                     /gene_synonym="Hs.22998; PTHSL2; SCZD17"
                     /inference="alignment:Splign:2.1.0"
     exon            1894..1905
                     /gene="NRXN1"
                     /gene_synonym="Hs.22998; PTHSL2; SCZD17"
                     /inference="alignment:Splign:2.1.0"
     exon            1906..2207
                     /gene="NRXN1"
                     /gene_synonym="Hs.22998; PTHSL2; SCZD17"
                     /inference="alignment:Splign:2.1.0"
     exon            2208..2231
                     /gene="NRXN1"
                     /gene_synonym="Hs.22998; PTHSL2; SCZD17"
                     /inference="alignment:Splign:2.1.0"
     exon            2232..2393
                     /gene="NRXN1"
                     /gene_synonym="Hs.22998; PTHSL2; SCZD17"
                     /inference="alignment:Splign:2.1.0"
     exon            2394..2832
                     /gene="NRXN1"
                     /gene_synonym="Hs.22998; PTHSL2; SCZD17"
                     /inference="alignment:Splign:2.1.0"
     exon            2833..3216
                     /gene="NRXN1"
                     /gene_synonym="Hs.22998; PTHSL2; SCZD17"
                     /inference="alignment:Splign:2.1.0"
     exon            3217..3420
                     /gene="NRXN1"
                     /gene_synonym="Hs.22998; PTHSL2; SCZD17"
                     /inference="alignment:Splign:2.1.0"
     exon            3421..3447
                     /gene="NRXN1"
                     /gene_synonym="Hs.22998; PTHSL2; SCZD17"
                     /inference="alignment:Splign:2.1.0"
     exon            3448..3570
                     /gene="NRXN1"
                     /gene_synonym="Hs.22998; PTHSL2; SCZD17"
                     /inference="alignment:Splign:2.1.0"
     exon            3571..3952
                     /gene="NRXN1"
                     /gene_synonym="Hs.22998; PTHSL2; SCZD17"
                     /inference="alignment:Splign:2.1.0"
     exon            3953..4143
                     /gene="NRXN1"
                     /gene_synonym="Hs.22998; PTHSL2; SCZD17"
                     /inference="alignment:Splign:2.1.0"
     exon            4144..4317
                     /gene="NRXN1"
                     /gene_synonym="Hs.22998; PTHSL2; SCZD17"
                     /inference="alignment:Splign:2.1.0"
     exon            4318..4437
                     /gene="NRXN1"
                     /gene_synonym="Hs.22998; PTHSL2; SCZD17"
                     /inference="alignment:Splign:2.1.0"
     exon            4438..4619
                     /gene="NRXN1"
                     /gene_synonym="Hs.22998; PTHSL2; SCZD17"
                     /inference="alignment:Splign:2.1.0"
     exon            4620..4791
                     /gene="NRXN1"
                     /gene_synonym="Hs.22998; PTHSL2; SCZD17"
                     /inference="alignment:Splign:2.1.0"
     exon            4792..4881
                     /gene="NRXN1"
                     /gene_synonym="Hs.22998; PTHSL2; SCZD17"
                     /inference="alignment:Splign:2.1.0"
     exon            4882..5201
                     /gene="NRXN1"
                     /gene_synonym="Hs.22998; PTHSL2; SCZD17"
                     /inference="alignment:Splign:2.1.0"
     exon            5202..5289
                     /gene="NRXN1"
                     /gene_synonym="Hs.22998; PTHSL2; SCZD17"
                     /inference="alignment:Splign:2.1.0"
     exon            5290..9038
                     /gene="NRXN1"
                     /gene_synonym="Hs.22998; PTHSL2; SCZD17"
                     /inference="alignment:Splign:2.1.0"
     regulatory      7176..7181
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NRXN1"
                     /gene_synonym="Hs.22998; PTHSL2; SCZD17"
                     /note="hexamer: AATATA"
     polyA_site      7193
                     /gene="NRXN1"
                     /gene_synonym="Hs.22998; PTHSL2; SCZD17"
     regulatory      8657..8662
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NRXN1"
                     /gene_synonym="Hs.22998; PTHSL2; SCZD17"
                     /note="hexamer: AATAAA"
     polyA_site      8678
                     /gene="NRXN1"
                     /gene_synonym="Hs.22998; PTHSL2; SCZD17"
                     /note="major polyA site"
     regulatory      9015..9020
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NRXN1"
                     /gene_synonym="Hs.22998; PTHSL2; SCZD17"
                     /note="hexamer: ATTAAA"
     polyA_site      9038
                     /gene="NRXN1"
                     /gene_synonym="Hs.22998; PTHSL2; SCZD17"
ORIGIN      
        1 gttctcccct cccgcactcc tggagataga cgctcaagcc tgacgcctct gaaaaggcag
       61 cagcagtcgc cttatccata gacctcagga aattcgcttt gaccgatgca tgcttcaggc
      121 tctggctcga gctgagggga ataaccgggc agatgtctga gtccttaaaa aagacaagat
      181 aaaaccaaaa tgcatcacca acacacaacc aattgatgtg ctggcatctt agcttggccc
      241 tgcagaggca gagagaaaag aagaggagct gttgcgattt ccacatctaa cagcttctgg
      301 ctatttcgca actgctgggt ttgagtccta ctgttgccgt ggctgaaaag agaagtaaaa
      361 tccaacttgt gagaccacgc acaaggaatt aggctttggc tgcagcggtg ctgaagccca
      421 tgctatgaca gaagaagcag gcctttgaag ctatcctcgg gttaagaaat ggtgccctaa
      481 agaggcaagc atccctttct ccgttctgct gtaagacacc acatctgaag gagacagact
      541 tgaggcattc acaagcatcc aaggatactg gctcactgct ggcttcctgt actttcttga
      601 gaaaagtgtt ttctttttac tgactctcac tgcttccttt tggcctggta ccactggtgg
      661 aacctggcag ataatgggat ttgaggagtt aagtatctgt tgacactgca gatactctta
      721 aattagttaa tcagactcaa atgggtcagt gctttccctt gatcttttgt tgaagccttt
      781 tccagaacgt tgtctccaaa gtgcatcctc cctttggtct ggaagttctg tggctaccct
      841 gtaccactgg ggtcaacgtt ttccagttgg aagaggacta cagggagaga gggcggggtt
      901 attggaagaa gaagaagaaa aaaagaacta gataaaggag ggcacatccc tctcactccc
      961 gcctttcccc ttacctttct gtctctcggg acccttattt cttcgtcacg gtgtccagga
     1021 ccattttgac cctgtcggcc ccggcacccc cccgccgcac cccagccccg agcatgggga
     1081 cggcgctgct ccagcgcggg ggctgttttc ttctgtgcct ctcgctgctg ctcctgggct
     1141 gctgggcgga gctgggcagc gggctggagt ttccgggcgc cgagggccaa tggacgcgct
     1201 tccccaagtg gaacgcctgc tgcgagagcg agatgagctt ccagctcaag actcgcagcg
     1261 cccgcggcct cgtgctctac ttcgacgacg agggcttctg cgacttcctg gagctgattc
     1321 tgacgcgcgg cggccgcctg cagctcagct tctccatctt ctgcgctgag cctgcgacgc
     1381 tcctggccga cacgccggtt aacgacggcg cctggcacag cgtgcgcatc cgccgccagt
     1441 tccgcaacac cacgctcttc atcgaccagg tggaggccaa gtgggtggag gtcaagtcca
     1501 agcgcaggga catgacggtg ttcagcggcc ttttcgtcgg ggggctgccc ccggaactgc
     1561 gcgccgcggc gctcaagctc accctggcct cggtgaggga gcgggagccc ttcaaggggt
     1621 ggattcgtga cgtgagggtc aactcctcgc aggtcctgcc cgtggacagc ggcgaggtga
     1681 agctggacga tgagccgccc aacagcggcg ggggaagccc gtgcgaggcg ggcgaggagg
     1741 gcgagggcgg ggtgtgcctc aacggaggtg tgtgctccgt ggtggacgac caggccgtgt
     1801 gcgactgctc gcgaaccggc ttccgcggca aggactgcag ccaagaagac aacaatgtgg
     1861 aaggtctggc gcacctgatg atgggcgacc aaggtaaaag taaaggaaaa gaagaatata
     1921 ttgccacgtt caaaggatct gaatacttct gctacgactt gtctcaaaac cccattcaaa
     1981 gcagcagtga tgaaataact ctgtcattta aaacccttca gaggaatgga ctgatgcttc
     2041 acactgggaa atcggctgat tatgtcaatc ttgccctgaa aaatggagct gtctctctgg
     2101 tcattaattt gggatcaggg gcctttgaag cactagtgga gcctgtgaat ggaaagttta
     2161 atgataatgc ctggcatgat gtgaaagtca ccaggaatct gcgtcagcac tcaggcattg
     2221 gacacgctat ggtgacaata tcagtggatg ggattcttac cacaacgggc tacacgcaag
     2281 aagattatac catgctgggg tctgatgact ttttctatgt tggaggcagt cccagcacag
     2341 ccgaccttcc agggtcacca gtcagtaaca actttatggg ctgtctcaaa gaggttgtat
     2401 ataaaaataa tgatgtgagg ctggaattat ctcgacttgc caagcaagga gatcctaaga
     2461 tgaagatcca tggagtggtg gcatttaaat gtgagaatgt tgcaacttta gacccaatca
     2521 cctttgaaac cccagagtct ttcatctctt tgcctaaatg gaatgcaaag aaaactggct
     2581 ccatatcatt tgatttccgt acaacagagc caaatggcct catcttattt agccatggca
     2641 agccaagaca tcagaaagat gccaagcacc cacagatgat aaaggtggac ttctttgcta
     2701 ttgagatgct agatggccac ctctacctcc tcctggacat ggggtcaggt actataaaaa
     2761 taaaagccct gttgaagaaa gtgaatgatg gagaatggta tcatgtggac ttccagagag
     2821 acggacggtc aggtaccatt tctgtcaaca cgttgcgtac tccctacact gctcctggtg
     2881 agagtgagat tctggacctg gatgatgagt tgtacctggg ggggctgcca gaaaataaag
     2941 ctggccttgt cttccccacc gaggtgtgga ctgctctgct caactatggc tacgtgggct
     3001 gcatcaggga tttgttcatc gatggccaaa gcaaagatat ccggcaaatg gctgaagttc
     3061 aaagtactgc tggagtgaag ccttcctgct caaaggaaac agcaaaaccg tgccttagca
     3121 acccttgcaa aaacaatggc atgtgcaggg atgggtggaa cagatatgtc tgtgattgtt
     3181 ccggaacagg ctatcttggc aggtcctgtg agagagaggc aacggttttg agctatgatg
     3241 ggagcatgtt tatgaaaatt cagctccccg tagtcatgca tacggaggct gaggatgttt
     3301 ccttacggtt ccgatcccag cgtgcatatg gcattctgat ggcaaccact tctagagact
     3361 ctgctgacac cctccgcctg gagctagacg caggacgtgt gaaactgacg gtcaatctag
     3421 attgtatcag gattaactgt aattccagca aaggtcccga gactcttttt gctggctata
     3481 acctcaatga taacgagtgg cacacagtgc gtgtagttcg gcgtggaaaa agtttaaagt
     3541 taacagtgga tgaccaacag gccatgacag gtcaaatggc aggtgatcat actaggctgg
     3601 agttccataa catagagact ggcatcatca cagaacgacg gtatctttct tctgtcccct
     3661 ccaacttcat tggacacctg cagagcttga catttaatgg aatggcatac attgacctgt
     3721 gtaaaaatgg cgacatagat tactgtgagc ttaatgccag atttggcttc aggaacatca
     3781 tagcagatcc tgtcaccttc aagaccaaat cgagctatgt tgccttagct accttgcaag
     3841 cctacacttc tatgcatctt tttttccagt tcaagacaac atccctagat ggattaattc
     3901 tatataacag tggggatgga aatgacttta ttgtggttga attagttaaa gggtacttac
     3961 attacgtgtt tgatttggga aatggtgcta acctcatcaa aggaagctca aataaacctc
     4021 tcaatgacaa tcagtggcac aacgtgatga tatcaaggga caccagcaac ctccacactg
     4081 taaagattga cacaaaaatc acaacgcaaa tcaccgccgg agccaggaac ttagacctca
     4141 agagtgactt atatatagga ggagtagcta aagaaacata caaatcctta ccaaaacttg
     4201 tacatgccaa agaaggcttt caaggctgcc tggcatcagt tgatttaaat ggacggcttc
     4261 cggacctcat ctccgatgct cttttctgca acggacagat cgagagagga tgtgaagggc
     4321 ccagcacaac ctgccaagag gactcatgtt ccaatcaagg tgtgtgcttg caacaatggg
     4381 atggcttcag ctgtgactgt agtatgactt ccttcagtgg accactctgc aatgaccctg
     4441 ggacgacata tatctttagc aaaggtggtg gacaaatcac gtataagtgg cctcctaatg
     4501 accgacccag tacacgagca gacagactgg ccataggttt tagcactgtt cagaaagaag
     4561 ccgtattggt gcgagtggac agttcttcag gcttgggtga ctacctagaa ctgcatatac
     4621 accagggaaa aattggagtt aagtttaatg ttgggacaga tgacatcgcc attgaagaat
     4681 ccaatgcaat cattaatgat gggaaatacc atgtagttcg tttcacgagg agtggtggca
     4741 atgccacgtt gcaggtggac agctggccag tgatcgagcg ctaccctgca ggaaacaatg
     4801 ataacgagcg cctggcgatt gctagacagc gaattccata tcgacttggt cgagtagttg
     4861 atgaatggct actcgacaaa gggcgtcagc tcacaatctt caatagccaa gcaaccataa
     4921 taattggcgg gaaagagcag ggccagccct tccagggcca gctctctggg ctgtactaca
     4981 atggcttgaa agttctgaat atggcagccg aaaacgatgc caacatcgcc atagtgggaa
     5041 atgtgagact ggttggtgaa gtgccttcct ctatgacaac tgagtcaaca gccactgcca
     5101 tgcaatcaga gatgtccaca tcaattatgg agactaccac gaccctggct actagcacag
     5161 ccagaagagg aaagcccccg acaaaagaac ccattagcca gaccacagat gacatccttg
     5221 tggcctcagc agagtgtccc agcgatgatg aggacattga cccctgtgag ccgagctcag
     5281 gtgggttagc caacccaacc cgagcaggcg gcagagagcc gtatccaggc tcagcagaag
     5341 tgatccggga gtccagcagc accacgggta tggtcgttgg gatagtagcc gctgccgccc
     5401 tgtgcatcct tatcctcctc tatgccatgt acaagtacag aaaccgggat gaaggctcat
     5461 accatgtgga cgagagtcga aactacatca gtaactcagc acagtccaat ggggctgttg
     5521 taaaggagaa acaacccagc agtgcgaaaa gctccaacaa aaataagaaa aacaaggata
     5581 aagagtatta tgtctgatcc caagatctta aatggacact tgtatagaaa tagtcttcat
     5641 tttatctgag acataatata aacttattta ctttcctttt tatgaagcac atacaaaaga
     5701 agacagggaa tgcaatcagg aaggaaagac tttttaaaaa ataaaaacaa gtatctcatg
     5761 ctcttgtttc tcaaaaaaga aaaacaaaaa acaaaaaaca ggggccaata aattccctaa
     5821 catccacagt gttttcattt actctgcttg tctttatgtt gctggaacat ttctaaaaga
     5881 cagtgatgac cgcacgcatt cataaagcaa aggagtacta cagcatcaag gcacaacaca
     5941 aaaaccaaca caaaacataa cacaaaaaag aagctaccta tgatcctgga tttagccaaa
     6001 gtgctagcgc tttcctgaga agtcagtcca attgccagag aagactgtcc ttttgagtga
     6061 ctcaacctgc aaacctttaa gagtttgccg cctggtgcaa ctggagcagt ggttggaact
     6121 tgcatttgaa acaaagtgct ggcttttttg aagacttgtg taggaacaca ttcaaaaagc
     6181 ccctttctgg ttgtgagaga ggaaaaaaaa gtatggaggc cttattttca aaaatgtgaa
     6241 atataaggca cgttttcaca caaaatttca aaacaaaaac aagagggcat agatgcaatc
     6301 attgggaaat tttcatgcac gcttattatg ttattacata tgtttatata aaatccatct
     6361 ctgtgtgctt tctggactgt gataagtgac gttttatagc ctgttgtata gaaaatgcaa
     6421 aatatatctc tgctcttcag ccatttttgg taaattcaat gttataagtg ttgctaagta
     6481 tagggagttt tatgacatca gagcaacaat tatttcagtt gggtttttct ttttttttgc
     6541 caccattata aattgccaca attactttta ttttttaaag aaattacagt gtagtgttta
     6601 ttctaaggaa gatatgtatg aatgtatata caaagactca gctacttctt ttcttatatg
     6661 tacagccttc attctgttgc aattaagttt tagtacttgt atgaaaggtg tgaattagaa
     6721 agtcacatat atacatatgt atcttataat cttttctccc tgaaatactc acattcccac
     6781 atacattcac tattttcaca cacacacaca cacacacaca cacacacaca cacacacaca
     6841 cacgaatcca cagcaatcca tcagatatgc tggaagatcc aaacgtgcat acagtagcaa
     6901 atatttattg acaaattgaa aagcaggaag gaagagggtt gtgccaaggt attgatgaca
     6961 aatggggtga tttgcttcat tgagatcttg ctcccaggta accttaagaa gattttagtc
     7021 cctaaagaaa tgaacctttc cttatcaaat agaatatcac tgatatactg ctgcatgaat
     7081 aagaaccatt atgtgggcag gttatggaag caaaattggt taatctacac cttaactctg
     7141 gctgctgcaa ttgaaaactt tctttctaat aaaataatat atatatctct gaatctgatg
     7201 tgcatgatga acatttctgg aaagtaaaca ctttctctcg actaagaaag tatttttcag
     7261 taatttttga ttctgtatta ctacccactt aaaaaatcat tctctgttaa ttattgacta
     7321 tgttttaact tttctggttt tacatatata tcattaaata tagttttagt ttaacacatt
     7381 aagtagtgga aaagtataga ttatacactt gttttgcata ttattcaatg atgttcacag
     7441 caataaatta ctaatatgag aaggtagtgc aaaaaatgca ttattacatt gcaagttgat
     7501 gtttcaggaa aaattggttg cattacttga ataccttcta aaagaacgat aaaaaatttt
     7561 gtagaagaga agaatatctt tccaattagc tttaatcagc aaaggcaaaa aatgcaaaac
     7621 atcttttgtg atactttagt tgatgaaaaa aaagccaaga agtggcctgg atttcagaca
     7681 tacagcacct tagaaatatg tataattcat agcaaatcac cactatctat ggttgccaag
     7741 taattgttgt gtacaaagaa atttagtggc tttttatgac ccagaaagta gaaggtataa
     7801 aatgaaaatt taatatccat tgcttctttt ctagattttc atttttttta atttgccaca
     7861 ttaaataatt cacgagtctt ttcttttccc ccgtctcatt cagtcttact cagggaaagc
     7921 cagttaaata agaagggaag tgtaaaacta ctaatttaca gcttgaaggg tttttttttt
     7981 taagattgct tttctatttt atctattaag cttgaaccag tttccatctg gaaagattct
     8041 aaaatataat agaaaataaa ttgaataatg gtaattttaa gcagtatatc tatgaaatgt
     8101 gttgtttggg attgatttca atgtttctcc ccagcgtaaa ttttaaagtt acatgcaatg
     8161 attgatatag tttattatca gcaaaattat ttctaatatt attggcttac acaaactcta
     8221 gcaaaaatta ttttttcctc aatggaaaaa gaaaagagag gaacagagtt agaaagagaa
     8281 aatgacttga atcccaagta tcttcatacc aaacatatca gggttccctg catagtttgc
     8341 agttgattga ggatttcaca cgacttggca taaatttgat tacatctcta gactgtaact
     8401 tttctgattg attatgcttc ttttttatcc atgtaatgtg ttgccttttt taaaagaaca
     8461 aatactacaa taacgtgtga agtggtcaat ccctaagaca tcaaagaaag gccacataac
     8521 cctacccttc tagtgctttg tgtgattggg tatcaaaggg gttttcttct tctttttttg
     8581 tttgtttgtt tttctgttgc aaggccaatt tatccattat tggaaatgct aagcaagtgg
     8641 aatttactgt tttgttaata aaatatttct taatacaact gtgatttatt gattttaaaa
     8701 attatatgcg gcaccatttc gtgcttaagt gggaaagtaa agaaggctgc atttgaaggg
     8761 aagacccatc cttatattat agaggaagaa tgttgactct cttttttctg gatttaacta
     8821 gtcattaaag acctctatat tcatacactt aaagatgtag ccattaaatg tacttttgtt
     8881 atatttgttc atcttgatat gccagaaacc ataaactcat acagtattag ttgcaaacaa
     8941 tattaaaata ctaatttcat aaatatgctt tttctctata aaagacaatg gaatccaagt
     9001 taagtaaacc tgtgattaaa ttccaacact atcacata
//
