LOCUS       NM_198465               8066 bp    mRNA    linear   PRI 04-OCT-2024
DEFINITION  Homo sapiens Nik related kinase (NRK), mRNA.
ACCESSION   NM_198465 XM_114703
VERSION     NM_198465.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8066)
  AUTHORS   He,W., Tian,Z., Dong,B., Cao,Y., Hu,W., Wu,P., Yu,L., Zhang,X. and
            Guo,S.
  TITLE     Identification and functional activity of Nik related kinase (NRK)
            in benign hyperplastic prostate
  JOURNAL   J Transl Med 22 (1), 255 (2024)
   PUBMED   38459501
  REMARK    GeneRIF: Identification and functional activity of Nik related
            kinase (NRK) in benign hyperplastic prostate.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8066)
  AUTHORS   Wei,T., Song,J., Liang,K., Li,L., Mo,X., Huang,Z., Chen,G., Mao,N.
            and Yang,J.
  TITLE     Identification of a novel therapeutic candidate, NRK, in primary
            cancer-associated fibroblasts of lung adenocarcinoma
            microenvironment
  JOURNAL   J Cancer Res Clin Oncol 147 (4), 1049-1064 (2021)
   PUBMED   33387038
  REMARK    GeneRIF: Identification of a novel therapeutic candidate, NRK, in
            primary cancer-associated fibroblasts of lung adenocarcinoma
            microenvironment.
REFERENCE   3  (bases 1 to 8066)
  AUTHORS   Naito,S., Fukushima,T., Endo,A., Denda,K. and Komada,M.
  TITLE     Nik-related kinase is targeted for proteasomal degradation by the
            chaperone-dependent ubiquitin ligase CHIP
  JOURNAL   FEBS Lett 594 (11), 1778-1786 (2020)
   PUBMED   32162334
  REMARK    GeneRIF: Nik-related kinase is targeted for proteasomal degradation
            by the chaperone-dependent ubiquitin ligase CHIP.
REFERENCE   4  (bases 1 to 8066)
  AUTHORS   Yanagawa,T., Denda,K., Inatani,T., Fukushima,T., Tanaka,T.,
            Kumaki,N., Inagaki,Y. and Komada,M.
  TITLE     Deficiency of X-Linked Protein Kinase Nrk during Pregnancy Triggers
            Breast Tumor in Mice
  JOURNAL   Am J Pathol 186 (10), 2751-2760 (2016)
   PUBMED   27634343
  REMARK    GeneRIF: The results suggest that Nrk suppresses the excessive
            proliferation of mammary epithelial cells during pregnancy, and the
            impairment of this regulatory system leads to frequent occurrence
            of breast tumor in Nrk mutant mice.
REFERENCE   5  (bases 1 to 8066)
  AUTHORS   Ross,M.T., Grafham,D.V., Coffey,A.J., Scherer,S., McLay,K.,
            Muzny,D., Platzer,M., Howell,G.R., Burrows,C., Bird,C.P.,
            Frankish,A., Lovell,F.L., Howe,K.L., Ashurst,J.L., Fulton,R.S.,
            Sudbrak,R., Wen,G., Jones,M.C., Hurles,M.E., Andrews,T.D.,
            Scott,C.E., Searle,S., Ramser,J., Whittaker,A., Deadman,R.,
            Carter,N.P., Hunt,S.E., Chen,R., Cree,A., Gunaratne,P., Havlak,P.,
            Hodgson,A., Metzker,M.L., Richards,S., Scott,G., Steffen,D.,
            Sodergren,E., Wheeler,D.A., Worley,K.C., Ainscough,R.,
            Ambrose,K.D., Ansari-Lari,M.A., Aradhya,S., Ashwell,R.I.,
            Babbage,A.K., Bagguley,C.L., Ballabio,A., Banerjee,R., Barker,G.E.,
            Barlow,K.F., Barrett,I.P., Bates,K.N., Beare,D.M., Beasley,H.,
            Beasley,O., Beck,A., Bethel,G., Blechschmidt,K., Brady,N.,
            Bray-Allen,S., Bridgeman,A.M., Brown,A.J., Brown,M.J., Bonnin,D.,
            Bruford,E.A., Buhay,C., Burch,P., Burford,D., Burgess,J.,
            Burrill,W., Burton,J., Bye,J.M., Carder,C., Carrel,L., Chako,J.,
            Chapman,J.C., Chavez,D., Chen,E., Chen,G., Chen,Y., Chen,Z.,
            Chinault,C., Ciccodicola,A., Clark,S.Y., Clarke,G., Clee,C.M.,
            Clegg,S., Clerc-Blankenburg,K., Clifford,K., Cobley,V., Cole,C.G.,
            Conquer,J.S., Corby,N., Connor,R.E., David,R., Davies,J., Davis,C.,
            Davis,J., Delgado,O., Deshazo,D., Dhami,P., Ding,Y., Dinh,H.,
            Dodsworth,S., Draper,H., Dugan-Rocha,S., Dunham,A., Dunn,M.,
            Durbin,K.J., Dutta,I., Eades,T., Ellwood,M., Emery-Cohen,A.,
            Errington,H., Evans,K.L., Faulkner,L., Francis,F., Frankland,J.,
            Fraser,A.E., Galgoczy,P., Gilbert,J., Gill,R., Glockner,G.,
            Gregory,S.G., Gribble,S., Griffiths,C., Grocock,R., Gu,Y.,
            Gwilliam,R., Hamilton,C., Hart,E.A., Hawes,A., Heath,P.D.,
            Heitmann,K., Hennig,S., Hernandez,J., Hinzmann,B., Ho,S., Hoffs,M.,
            Howden,P.J., Huckle,E.J., Hume,J., Hunt,P.J., Hunt,A.R.,
            Isherwood,J., Jacob,L., Johnson,D., Jones,S., de Jong,P.J.,
            Joseph,S.S., Keenan,S., Kelly,S., Kershaw,J.K., Khan,Z.,
            Kioschis,P., Klages,S., Knights,A.J., Kosiura,A., Kovar-Smith,C.,
            Laird,G.K., Langford,C., Lawlor,S., Leversha,M., Lewis,L., Liu,W.,
            Lloyd,C., Lloyd,D.M., Loulseged,H., Loveland,J.E., Lovell,J.D.,
            Lozado,R., Lu,J., Lyne,R., Ma,J., Maheshwari,M., Matthews,L.H.,
            McDowall,J., McLaren,S., McMurray,A., Meidl,P., Meitinger,T.,
            Milne,S., Miner,G., Mistry,S.L., Morgan,M., Morris,S., Muller,I.,
            Mullikin,J.C., Nguyen,N., Nordsiek,G., Nyakatura,G., O'Dell,C.N.,
            Okwuonu,G., Palmer,S., Pandian,R., Parker,D., Parrish,J.,
            Pasternak,S., Patel,D., Pearce,A.V., Pearson,D.M., Pelan,S.E.,
            Perez,L., Porter,K.M., Ramsey,Y., Reichwald,K., Rhodes,S.,
            Ridler,K.A., Schlessinger,D., Schueler,M.G., Sehra,H.K.,
            Shaw-Smith,C., Shen,H., Sheridan,E.M., Shownkeen,R., Skuce,C.D.,
            Smith,M.L., Sotheran,E.C., Steingruber,H.E., Steward,C.A.,
            Storey,R., Swann,R.M., Swarbreck,D., Tabor,P.E., Taudien,S.,
            Taylor,T., Teague,B., Thomas,K., Thorpe,A., Timms,K., Tracey,A.,
            Trevanion,S., Tromans,A.C., d'Urso,M., Verduzco,D., Villasana,D.,
            Waldron,L., Wall,M., Wang,Q., Warren,J., Warry,G.L., Wei,X.,
            West,A., Whitehead,S.L., Whiteley,M.N., Wilkinson,J.E.,
            Willey,D.L., Williams,G., Williams,L., Williamson,A.,
            Williamson,H., Wilming,L., Woodmansey,R.L., Wray,P.W., Yen,J.,
            Zhang,J., Zhou,J., Zoghbi,H., Zorilla,S., Buck,D., Reinhardt,R.,
            Poustka,A., Rosenthal,A., Lehrach,H., Meindl,A., Minx,P.J.,
            Hillier,L.W., Willard,H.F., Wilson,R.K., Waterston,R.H., Rice,C.M.,
            Vaudin,M., Coulson,A., Nelson,D.L., Weinstock,G., Sulston,J.E.,
            Durbin,R., Hubbard,T., Gibbs,R.A., Beck,S., Rogers,J. and
            Bentley,D.R.
  TITLE     The DNA sequence of the human X chromosome
  JOURNAL   Nature 434 (7031), 325-337 (2005)
   PUBMED   15772651
REFERENCE   6  (bases 1 to 8066)
  AUTHORS   Nakano,K., Kanai-Azuma,M., Kanai,Y., Moriyama,K., Yazaki,K.,
            Hayashi,Y. and Kitamura,N.
  TITLE     Cofilin phosphorylation and actin polymerization by NRK/NESK, a
            member of the germinal center kinase family
  JOURNAL   Exp Cell Res 287 (2), 219-227 (2003)
   PUBMED   12837278
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from Z70274.1, BX538345.1, Z69734.2,
            KF459287.1, Z68339.2 and Z83850.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_198465.3.
            
            Summary: The mouse ortholog of this gene encodes a protein kinase
            required for JNK activation. The encoded protein may be involved in
            the induction of actin polymerization in late
            embryogenesis.[provided by RefSeq, Jun 2010].
            
            ##Evidence-Data-START##
            Transcript exon combination :: BX538345.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968968, SAMEA2142853
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000243300.14/ ENSP00000434830.1
            RefSeq Select criteria :: based on conservation, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-4                 Z70274.1           30079-30082         c
            5-761               BX538345.1         3-759
            762-762             Z69734.2           19646-19646
            763-1674            BX538345.1         761-1672
            1675-1675           KF459287.1         27-27
            1676-2260           Z68339.2           11245-11829
            2261-2420           Z83850.1           102-261
            2421-3718           BX538345.1         2419-3716
            3719-3719           Z83850.1           24864-24864
            3720-3948           BX538345.1         3718-3946
            3949-3949           Z83850.1           25819-25819
            3950-5899           BX538345.1         3948-5897
            5900-8064           Z83850.1           46951-49115
            8065-8066           BX538345.1         8063-8064
FEATURES             Location/Qualifiers
     source          1..8066
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq22.3"
     gene            1..8066
                     /gene="NRK"
                     /gene_synonym="NESK"
                     /note="Nik related kinase"
                     /db_xref="GeneID:203447"
                     /db_xref="HGNC:HGNC:25391"
                     /db_xref="MIM:300791"
     exon            1..364
                     /gene="NRK"
                     /gene_synonym="NESK"
                     /inference="alignment:Splign:2.1.0"
     CDS             308..5056
                     /gene="NRK"
                     /gene_synonym="NESK"
                     /EC_number="2.7.11.1"
                     /codon_start=1
                     /product="nik-related protein kinase"
                     /protein_id="NP_940867.2"
                     /db_xref="CCDS:CCDS65305.1"
                     /db_xref="GeneID:203447"
                     /db_xref="HGNC:HGNC:25391"
                     /db_xref="MIM:300791"
                     /translation="MAGPGGWRDREVTDLGHLPDPTGIFSLDKTIGLGTYGRIYLGLH
                     EKTGAFTAVKVMNARKTPLPEIGRRVRVNKYQKSVGWRYSDEEEDLRTELNLLRKYSF
                     HKNIVSFYGAFFKLSPPGQRHQLWMVMELCAAGSVTDVVRMTSNQSLKEDWIAYICRE
                     ILQGLAHLHAHRVIHRDIKGQNVLLTHNAEVKLVDFGVSAQVSRTNGRRNSFIGTPYW
                     MAPEVIDCDEDPRRSYDYRSDVWSVGITAIEMAEGAPPLCNLQPLEALFVILRESAPT
                     VKSSGWSRKFHNFMEKCTIKNFLFRPTSANMLQHPFVRDIKNERHVVESLTRHLTGII
                     KKRQKKGIPLIFEREEAIKEQYTVRRFRGPSCTHELLRLPTSSRCRPLRVLHGEPSQP
                     RWLPDREEPQVQALQQLQGAARVFMPLQALDSAPKPLKGQAQAPQRLQGAARVFMPLQ
                     AQVKAKASKPLQMQIKAPPRLRRAARVLMPLQAQVRAPRLLQVQSQVSKKQQAQTQTS
                     EPQDLDQVPEEFQGQDQVPEQQRQGQAPEQQQRHNQVPEQELEQNQAPEQPEVQEQAA
                     EPAQAETEAEEPESLRVNAQVFLPLLSQDHHVLLPLHLDTQVLIPVEGQTEGSPQAQA
                     WTLEPPQAIGSVQALIEGLSRDLLRAPNSNNSKPLGPLQTLMENLSSNRFYSQPEQAR
                     EKKSKVSTLRQALAKRLSPKRFRAKSSWRPEKLELSDLEARRQRRQRRWEDIFNQHEE
                     ELRQVDKDKEDESSDNDEVFHSIQAEVQIEPLKPYISNPKKIEVQERSPSVPNNQDHA
                     HHVKFSSSVPQRSLLEQAQKPIDIRQRSSQNRQNWLAASESSSEEESPVTGRRSQSSP
                     PYSTIDQKLLVDIHVPDGFKVGKISPPVYLTNEWVGYNALSEIFRNDWLTPAPVIQPP
                     EEDGDYVELYDASADTDGDDDDESNDTFEDTYDHANGNDDLDNQVDQANDVCKDHDDD
                     NNKFVDDVNNNYYEAPSCPRASYGRDGSCKQDGYDGSRGKEEAYRGYGSHTANRSHGG
                     SAASEDNAAIGDQEEHAANIGSERRGSEGDGGKGVVRTSEESGALGLNGEENCSETDG
                     PGLKRPASQDFEYLQEEPGGGNEASNAIDSGAAPSAPDHESDNKDISESSTQSDFSAN
                     HSSPSKGSGMSADANFASAILYAGFVEVPEESPKQPSEVNVNPLYVSPACKKPLIHMY
                     EKEFTSEICCGSLWGVNLLLGTRSNLYLMDRSGKADITKLIRRRPFRQIQVLEPLNLL
                     ITISGHKNRLRVYHLTWLRNKILNNDPESKRRQEEMLKTEEACKAIDKLTGCEHFSVL
                     QHEETTYIAIALKSSIHLYAWAPKSFDESTAIKVCIDQSADSEGDYMSYQAYIRILAK
                     IQAADPVNRFKRPDELLHLLKLKVFPTLDHKPVTVDLAIGSEKRLKIFFSSADGYHLI
                     DAESEVMSDVTLPKNPLEIIIPQNIIILPDCLGIGMMLTFNAEALSVEANEQLFKKIL
                     EMWKDIPSSIAFECTQRTTGWGQKAIEVRSLQSRVLESELKRRSIKKLRFLCTRGDKL
                     FFTSTLRNHHSRVYFMTLGKLEELQSNYDV"
     exon            365..430
                     /gene="NRK"
                     /gene_synonym="NESK"
                     /inference="alignment:Splign:2.1.0"
     exon            431..487
                     /gene="NRK"
                     /gene_synonym="NESK"
                     /inference="alignment:Splign:2.1.0"
     exon            488..559
                     /gene="NRK"
                     /gene_synonym="NESK"
                     /inference="alignment:Splign:2.1.0"
     exon            560..685
                     /gene="NRK"
                     /gene_synonym="NESK"
                     /inference="alignment:Splign:2.1.0"
     exon            686..796
                     /gene="NRK"
                     /gene_synonym="NESK"
                     /inference="alignment:Splign:2.1.0"
     exon            797..887
                     /gene="NRK"
                     /gene_synonym="NESK"
                     /inference="alignment:Splign:2.1.0"
     exon            888..1018
                     /gene="NRK"
                     /gene_synonym="NESK"
                     /inference="alignment:Splign:2.1.0"
     exon            1019..1073
                     /gene="NRK"
                     /gene_synonym="NESK"
                     /inference="alignment:Splign:2.1.0"
     exon            1074..1152
                     /gene="NRK"
                     /gene_synonym="NESK"
                     /inference="alignment:Splign:2.1.0"
     exon            1153..1328
                     /gene="NRK"
                     /gene_synonym="NESK"
                     /inference="alignment:Splign:2.1.0"
     exon            1329..1392
                     /gene="NRK"
                     /gene_synonym="NESK"
                     /inference="alignment:Splign:2.1.0"
     exon            1393..2548
                     /gene="NRK"
                     /gene_synonym="NESK"
                     /inference="alignment:Splign:2.1.0"
     exon            2549..2656
                     /gene="NRK"
                     /gene_synonym="NESK"
                     /inference="alignment:Splign:2.1.0"
     exon            2657..2724
                     /gene="NRK"
                     /gene_synonym="NESK"
                     /inference="alignment:Splign:2.1.0"
     exon            2725..2819
                     /gene="NRK"
                     /gene_synonym="NESK"
                     /inference="alignment:Splign:2.1.0"
     exon            2820..2917
                     /gene="NRK"
                     /gene_synonym="NESK"
                     /inference="alignment:Splign:2.1.0"
     exon            2918..3282
                     /gene="NRK"
                     /gene_synonym="NESK"
                     /inference="alignment:Splign:2.1.0"
     exon            3283..3619
                     /gene="NRK"
                     /gene_synonym="NESK"
                     /inference="alignment:Splign:2.1.0"
     exon            3620..3806
                     /gene="NRK"
                     /gene_synonym="NESK"
                     /inference="alignment:Splign:2.1.0"
     exon            3807..3962
                     /gene="NRK"
                     /gene_synonym="NESK"
                     /inference="alignment:Splign:2.1.0"
     exon            3963..4106
                     /gene="NRK"
                     /gene_synonym="NESK"
                     /inference="alignment:Splign:2.1.0"
     exon            4107..4265
                     /gene="NRK"
                     /gene_synonym="NESK"
                     /inference="alignment:Splign:2.1.0"
     exon            4266..4366
                     /gene="NRK"
                     /gene_synonym="NESK"
                     /inference="alignment:Splign:2.1.0"
     exon            4367..4510
                     /gene="NRK"
                     /gene_synonym="NESK"
                     /inference="alignment:Splign:2.1.0"
     exon            4511..4660
                     /gene="NRK"
                     /gene_synonym="NESK"
                     /inference="alignment:Splign:2.1.0"
     exon            4661..4820
                     /gene="NRK"
                     /gene_synonym="NESK"
                     /inference="alignment:Splign:2.1.0"
     exon            4821..4960
                     /gene="NRK"
                     /gene_synonym="NESK"
                     /inference="alignment:Splign:2.1.0"
     exon            4961..8066
                     /gene="NRK"
                     /gene_synonym="NESK"
                     /inference="alignment:Splign:2.1.0"
     regulatory      8048..8053
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NRK"
                     /gene_synonym="NESK"
                     /note="hexamer: AATAAA"
     polyA_site      8066
                     /gene="NRK"
                     /gene_synonym="NESK"
                     /note="major polyA site"
ORIGIN      
        1 agacacagcg ctctcgacac ggagcaccct tctagcttct tcgtctccag gactgacgct
       61 caggctcctc tctcgcctta gcccaacttg ctttcccgcc tcgcaaactc cggtttccct
      121 ccactcccaa ctcttttcac tacacgtttc ccctcctcta tctcccacgc cacgaacccc
      181 gatccccaga ctcctctctc ccgccctcct ccttcctctc tcctcccttc aactcttcat
      241 ccgcttccac ctcagactct gcgcgcaccc aattcagtcg cccgctcccg ttcggctcct
      301 cgaagccatg gcgggacctg ggggctggag ggacagggag gtcacggatc tgggccacct
      361 gccggatcca actggaatat tctcactaga taaaaccatt ggccttggta cttatggcag
      421 aatctatttg ggacttcatg agaagactgg tgcatttaca gctgttaaag tgatgaacgc
      481 tcgtaagacc cctttacctg aaataggaag gcgagtgaga gtgaataaat atcaaaaatc
      541 tgttgggtgg agatacagtg atgaggaaga ggatctcagg actgaactca accttctgag
      601 gaagtactct ttccacaaaa acattgtgtc cttctatgga gcatttttca agctgagtcc
      661 ccctggtcag cggcaccaac tttggatggt gatggagtta tgtgcagcag gttcggtcac
      721 tgatgtagtg agaatgacca gtaatcagag tttaaaagaa gattggattg cttatatctg
      781 ccgagaaatc cttcagggct tagctcacct tcacgcacac cgagtaattc accgggacat
      841 caaaggtcag aatgtgctgc tgactcataa tgctgaagta aaactggttg attttggagt
      901 gagtgcccag gtgagcagaa ctaatggaag aaggaatagt ttcattggga caccatactg
      961 gatggcacct gaggtgattg actgtgatga ggacccaaga cgctcctatg attacagaag
     1021 tgatgtgtgg tctgtgggaa ttactgccat tgaaatggct gaaggagccc ctcctctgtg
     1081 taaccttcaa cccttggaag ctctcttcgt tattttgcgg gaatctgctc ccacagtcaa
     1141 atccagcgga tggtcccgta agttccacaa tttcatggaa aagtgtacga taaaaaattt
     1201 cctgtttcgt cctacttctg caaacatgct tcaacaccca tttgttcggg atataaaaaa
     1261 tgaacgacat gttgttgagt cattaacaag gcatcttact ggaatcatta aaaaaagaca
     1321 gaaaaaagga atacctttga tctttgaaag agaagaagct attaaggaac agtacaccgt
     1381 gagaagattc agaggaccct cttgcactca cgagcttctg agattgccaa ccagcagcag
     1441 atgcagacca cttagagtcc tgcatgggga accctctcag ccaaggtggc tacctgatcg
     1501 agaagagcca caggtccagg cacttcagca gctacaggga gcagccaggg tattcatgcc
     1561 actgcaggct ctggacagtg cacctaagcc tctaaagggg caggctcagg cacctcaacg
     1621 actacaaggg gcagctcggg tgttcatgcc actacaggct caggtgaagg ctaaggcctc
     1681 taaacctcta caaatgcaga ttaaggcacc tccacgacta cggagggcag ccagggtgct
     1741 catgccacta caggcacagg ttagggcacc taggcttctg caggtacagt cccaggtatc
     1801 caaaaagcag caggcccaga cccagacatc agaaccacaa gatttggacc aggtaccaga
     1861 ggaatttcag ggtcaagatc aggtacccga acaacaaagg cagggccagg cccctgaaca
     1921 acagcagagg cacaaccagg tgcctgaaca agagctggag cagaaccagg cacctgaaca
     1981 gccagaggta caggaacagg ctgccgagcc tgcacaggca gagactgagg cagaggaacc
     2041 tgagtcatta cgagtaaatg cccaggtatt tctgcccctg ctatcacaag atcaccatgt
     2101 gctgttgcca ctacatttgg atactcaggt gctcattcca gtagaggggc aaactgaagg
     2161 atcacctcag gcacaggctt ggacactaga acccccacag gcaattggct cagttcaagc
     2221 actgatagag ggactatcaa gagacttgct tcgggcacca aactcaaata actcaaagcc
     2281 acttggtccg ttgcaaaccc tgatggaaaa tctgtcatca aataggtttt actcacaacc
     2341 agaacaggca cgggagaaaa aatcaaaagt ttctactctg aggcaagcac tggcaaaaag
     2401 actatcacca aagaggttca gggcaaagtc atcatggaga cctgaaaagc ttgaactctc
     2461 ggatttagaa gcccgcaggc aaaggcgcca acgcagatgg gaagatatct ttaatcagca
     2521 tgaggaagaa ttgagacaag ttgataaaga caaagaagat gaatcatcag acaatgatga
     2581 agtatttcat tcgattcagg ctgaagtcca gatagagcca ttgaagccat acatttcaaa
     2641 tcctaaaaaa attgaggttc aagagagatc tccttctgtg cctaacaacc aggatcatgc
     2701 acatcatgtc aagttctctt caagcgttcc tcagcggtct cttttggaac aagctcagaa
     2761 gcccattgac atcagacaaa ggagttcgca aaatcgtcaa aattggctgg cagcatcaga
     2821 atcttcttct gaggaagaaa gtcctgtgac tggaaggagg tctcagtcat caccacctta
     2881 ttctactatt gatcagaagt tgctggttga catccatgtt ccagatggat ttaaagtagg
     2941 aaaaatatca ccccctgtat acttgacaaa cgaatgggta ggctataatg cactctctga
     3001 aatcttccgg aatgattggt taactccggc acctgtcatt cagccacctg aagaggatgg
     3061 tgattatgtt gaactctatg atgccagtgc tgatactgat ggtgatgatg atgatgagtc
     3121 taatgatact tttgaagata cctatgatca tgccaatggc aatgatgact tggataacca
     3181 ggttgatcag gctaatgatg tttgtaaaga ccatgatgat gacaacaata agtttgttga
     3241 tgatgtaaat aataattatt atgaggcgcc tagttgtcca agggcaagct atggcagaga
     3301 tggaagctgc aagcaagatg gttatgatgg aagtcgtgga aaagaggaag cctacagagg
     3361 ctatggaagc catacagcca atagaagcca tggaggaagt gcagccagtg aggacaatgc
     3421 agccattgga gatcaggaag aacatgcagc caatataggc agtgaaagaa gaggcagtga
     3481 gggtgatgga ggtaagggag tcgttcgaac cagtgaagag agtggagccc ttggactcaa
     3541 tggagaagaa aattgctcag agacagatgg tccaggattg aagagacctg cgtctcagga
     3601 ctttgaatat ctacaggagg agccaggtgg tggaaatgag gcctcaaatg ccattgactc
     3661 aggtgctgca ccgtcagcac ctgatcatga gagtgacaat aaggacatat cagaatcatc
     3721 aacacaatca gatttttctg ccaatcactc atctccttcc aaaggttctg ggatgtctgc
     3781 tgatgctaac tttgccagtg ccatcttata cgctggattc gtagaagtac ctgaggaatc
     3841 acctaagcaa ccctctgaag tcaatgttaa cccactctat gtctctcctg catgtaaaaa
     3901 accactaatc cacatgtatg aaaaggagtt cacttctgag atctgctgtg gttctttgtg
     3961 gggagtcaat ttgctgttgg gaacccgatc taatctatat ctgatggaca gaagtggaaa
     4021 ggctgacatt actaaactta taaggcgaag accattccgc cagattcaag tcttagagcc
     4081 actcaatttg ctgattacca tctcaggtca taagaacaga cttcgggtgt atcatctgac
     4141 ctggttgagg aacaagattt tgaataatga tccagaaagt aaaagaaggc aagaagaaat
     4201 gctgaagaca gaggaagcct gcaaagctat tgataagtta acaggctgtg aacacttcag
     4261 tgtcctccaa catgaagaaa caacatatat tgcaattgct ttgaaatcat caattcacct
     4321 ttatgcatgg gcaccaaagt cctttgatga aagcactgct attaaagtat gcattgatca
     4381 atcagcagac tctgaaggag actacatgtc ctatcaagcc tatatacgaa tactggcaaa
     4441 aatacaggca gctgatccag tgaaccggtt taagagacca gatgagctcc ttcatttgct
     4501 gaagctcaag gtatttccaa cacttgatca taagccagtg acagttgacc tggctattgg
     4561 ttctgaaaaa agactaaaga ttttcttcag ctcagcagat ggatatcacc tcatcgatgc
     4621 agaatctgag gttatgtctg atgtgaccct gccaaagaat cccctggaaa tcattatacc
     4681 acagaatatc atcattttac ctgattgctt gggaattggc atgatgctca ccttcaatgc
     4741 tgaagccctc tctgtggaag caaatgaaca actcttcaag aagatccttg aaatgtggaa
     4801 agacatacca tcttctatag cttttgaatg tacacagcga accacaggat ggggccaaaa
     4861 ggccattgaa gtgcgctctt tgcaatccag ggttctggaa agtgagctga agcgcaggtc
     4921 aattaagaag ctgagattcc tgtgcacccg gggtgacaag ctgttcttta cctctaccct
     4981 gcgcaatcac cacagccggg tttacttcat gacacttgga aaacttgaag agctccaaag
     5041 caattatgat gtctaaaagt ttccagtgat ttattaccac attataaaca tcatgtatag
     5101 gcagtctgca tcttcagatt tcagagatta aatgagtatt cagttttatt tttagtaaag
     5161 attaaatcca aaactttact tttaatgtag cacagaatag ttttaatgag aaatgcagct
     5221 ttatgtataa aattaactat agcaagctct aggtactcca atggtgtaca atgtcttttg
     5281 cacaaacttt gtaacttttg ttactgtgaa ttcaaacatt actctttgga cagtttggac
     5341 agtatctgta ttcagatttt acaacatgga gtaaagaaac ctgttatgaa ttagattaca
     5401 agcagccttc aaaagaattg gcactgggat aagatttttc agaaaaagaa aaacatcggc
     5461 aaactgtgtg tgatttttcc aaagctatat aaagaaccaa aggtttagtc aagaaacaaa
     5521 aatcttaaag attattataa cccagactaa ggttgaacaa cctgcatgcc cagagaaaac
     5581 tatggcgaca aaggggaaaa ggccaccact cgttttctca ctgattcatg ccaattaagc
     5641 ctacagttaa agaccagttt tgttcttttc acccattttt aagctggttt tctcctgata
     5701 agaagaaagg aagaaagccc cagacgcttg gtttttctca gaacccccaa aagatgtgca
     5761 atagctgttg ttacaaacca ccaaataata cagttgtgag cctgaataca ggactgaact
     5821 cctatacacg tgtactgtag aatgagtatt ttttaatacc ttaaggtagg cgtcaaattc
     5881 tactccccaa agcagagata gattgattta tcaaaattat tatctggcca acagtgtgac
     5941 tatcagacag catcaaatat ttgcccaatc caagattaga ctacacaaaa gcttccttcc
     6001 agtattaaac aaaaagaatt aaacataact atgaaaaaac tttgctaata tctgtgtttt
     6061 tcagatttca ttttttgtaa aatcagaaat taatctaaac atattcagtg ataagttcat
     6121 gtgtaacgac ttaatgttaa aggttaaaaa aaagatttca caaaatatac aactttcacc
     6181 atatatataa gcctgcaaaa ttagagtagt gaaagtcatg ctagtccatc acccaaatat
     6241 gttatagacg ccatagacag gtgatgtttg gtcacctatg gtaactgcta cctgatgaag
     6301 agcataattt ctgcatatcc atcctcaata ccatggtaaa ttctggggca atagagaagc
     6361 aacagaactg ccacaaagta tacctcaata taattcctct agttctgctt ctaaaatctg
     6421 aggacagtgc tagtgggaaa ataattttca aactacctgg ttaaccaaaa tacaaaagca
     6481 gctgactatg tgtgatttca taatagcaca tttcttgaca cttagtgcta gaaatgaaga
     6541 tttggatttt cctaacaact tacatcaaga atgtagtgta gctcattatt gagaatttag
     6601 gaaagcctga atccattaat taaggaaata aatgtgactc acatttcttt tactgtgaca
     6661 caataatgtg atcctaaaac tggcttatcc ttgagtgttt acaactcaaa caactttttg
     6721 aatgcagtag tttttttttt ttaaaaacaa acttttatgt caaatttttt ttcttagaag
     6781 tagtcttcat tattataaat ttgtacacca aaaggccatg gggaactttg tgcaagtacc
     6841 tcatcgctga gcaaatggag cttgctatgt tttaatttca gaaaatttcc tcatatacgt
     6901 agtgtgtaga atcaagtctt ttaataattc attttttctt cataatattt actcaaagtt
     6961 aagcttaaaa ataagtttta tcttaaaatc atatttgaag acagtaagac agtaaactat
     7021 tttaggaagt caacccccat tgcactctgt ggcagttatt ctggtaaaaa taggcaaaag
     7081 tgacctgaat ctacaatgat gtcccaaagt aaccaagtaa gagagattgt aaatgataaa
     7141 ccgagcttta aaggataaag tgttaataaa gaaaggaagc tgggcacatg tcaaaaaggg
     7201 agatcgaaat gttaggtaat catttagaaa ggacagaaaa tatttaaagt ggctcatagg
     7261 taatgaatat ttctgactta gatgtaaatc catctggaat ctttacatcc tttgccagct
     7321 gaaacaagaa agtgaaggga caatgatatt tcatggtcag tttattttgt aagagacaga
     7381 agaaattata tctatacatt accttgtagc agcagtacct ggaagcccca gcccgtcaca
     7441 gaagtgtgga ggggggctcc tgactagaca atttccctag cccttgtgat ttgaagcatg
     7501 aaagttctgg caggttatga gcagcactag ggataaagta tggttttatt ttggtgtaat
     7561 ttaggttttt caacaaagcc cttgtctaaa ataaaaggca ttattggaaa tatttgaaaa
     7621 ctagaaaatg atggataaaa gggctgataa gaaaatttct gactgtcagt agaagtgaga
     7681 taagatcctc agaggaaaca gtaagaaggg ataatcatta agatagtaaa acaggcaaag
     7741 cagaatcaca tgtgcacaca cacatacaca tgtaaacatt ggaatgcata agttttaata
     7801 ttttagcgct atcagtttct aaatgcatta attactaact gccctctccc aagattcatt
     7861 tagttcaaac agtatccgta aactaggaat aatgccacat gcattcaatg ggatctttta
     7921 agtactcttc agtttgttcc aagaaatgtg cctactgaaa tcaaattaat ttgtattcaa
     7981 tgtgtacttc aagactgcta attgtttcat ctgaaagcct acaatgaatc attgttcaac
     8041 cttgaaaaat aaaattttgt aaaaca
//
