LOCUS       NM_017970              14008 bp    mRNA    linear   PRI 10-JUN-2024
DEFINITION  Homo sapiens NRDE-2, necessary for RNA interference, domain
            containing (NRDE2), mRNA.
ACCESSION   NM_017970
VERSION     NM_017970.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 14008)
  AUTHORS   Wang,Y., Liu,X., Zuo,X., Wang,C., Zhang,Z., Zhang,H., Zeng,T.,
            Chen,S., Liu,M., Chen,H., Song,Q., Li,Q., Yang,C., Le,Y., Xing,J.,
            Zhang,H., An,J., Jia,W., Kang,L., Zhang,H., Xie,H., Ye,J., Wu,T.,
            He,F., Zhang,X., Li,Y. and Zhou,G.
  TITLE     NRDE2 deficiency impairs homologous recombination repair and
            sensitizes hepatocellular carcinoma to PARP inhibitors
  JOURNAL   Cell Genom 4 (5), 100550 (2024)
   PUBMED   38697125
  REMARK    GeneRIF: NRDE2 deficiency impairs homologous recombination repair
            and sensitizes hepatocellular carcinoma to PARP inhibitors.
REFERENCE   2  (bases 1 to 14008)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 14008)
  AUTHORS   Wang,J., Chen,J., Wu,G., Zhang,H., Du,X., Chen,S., Zhang,L.,
            Wang,K., Fan,J., Gao,S., Wu,X., Zhang,S., Kuai,B., Zhao,P., Chi,B.,
            Wang,L., Li,G., Wong,C.C.L., Zhou,Y., Li,J., Yun,C. and Cheng,H.
  TITLE     NRDE2 negatively regulates exosome functions by inhibiting MTR4
            recruitment and exosome interaction
  JOURNAL   Genes Dev 33 (9-10), 536-549 (2019)
   PUBMED   30842217
  REMARK    GeneRIF: NRDE2 interacts with MTR4's key residues, locks MTR4 in a
            closed conformation, and inhibits MTR4 interaction with the exosome
            as well as proteins important for MTR4 recruitment
REFERENCE   4  (bases 1 to 14008)
  AUTHORS   Jiao,A.L., Perales,R., Umbreit,N.T., Haswell,J.R., Piper,M.E.,
            Adams,B.D., Pellman,D., Kennedy,S. and Slack,F.J.
  TITLE     Human nuclear RNAi-defective 2 (NRDE2) is an essential RNA splicing
            factor
  JOURNAL   RNA 25 (3), 352-363 (2019)
   PUBMED   30538148
  REMARK    GeneRIF: Our work establishes a conserved role for human NRDE2 in
            RNA splicing, characterizes the severe genomic instability
            phenotypes observed upon loss of NRDE2, and highlights the direct
            regulation of CEP131 splicing as one of multiple mechanisms through
            which such phenotypes might be explained.
REFERENCE   5  (bases 1 to 14008)
  AUTHORS   Richard,P., Ogami,K., Chen,Y., Feng,S., Moresco,J.J., Yates,J.R.
            3rd and Manley,J.L.
  TITLE     NRDE-2, the human homolog of fission yeast Nrl1, prevents DNA
            damage accumulation in human cells
  JOURNAL   RNA Biol 15 (7), 868-876 (2018)
   PUBMED   29902117
  REMARK    GeneRIF: this study found that Mtr4 association with the human
            homolog of fission yeast Nrl1, NRDE-2, defines a novel function for
            Mtr4 in the DNA damage response pathway.
REFERENCE   6  (bases 1 to 14008)
  AUTHORS   Yashin,A.I., Wu,D., Arbeev,K.G. and Ukraintseva,S.V.
  TITLE     Joint influence of small-effect genetic variants on human longevity
  JOURNAL   Aging (Albany NY) 2 (9), 612-620 (2010)
   PUBMED   20834067
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK294958.1, BC008925.2, AW196499.1, AL161662.6 and AL355074.5.
            
            On Nov 22, 2018 this sequence version replaced NM_017970.3.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK294958.1, SRR1803616.261501.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000354366.8/ ENSP00000346335.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1557              AK294958.1         1-1557
            1558-3150           BC008925.2         1184-2776
            3151-3810           AK294958.1         3151-3810
            3811-3814           AW196499.1         17-20               c
            3815-11688          AL161662.6         85251-93124         c
            11689-14008         AL355074.5         179316-181635       c
FEATURES             Location/Qualifiers
     source          1..14008
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q32.11"
     gene            1..14008
                     /gene="NRDE2"
                     /gene_synonym="C14orf102"
                     /note="NRDE-2, necessary for RNA interference, domain
                     containing"
                     /db_xref="GeneID:55051"
                     /db_xref="HGNC:HGNC:20186"
                     /db_xref="MIM:618631"
     exon            1..101
                     /gene="NRDE2"
                     /gene_synonym="C14orf102"
                     /inference="alignment:Splign:2.1.0"
     CDS             38..3532
                     /gene="NRDE2"
                     /gene_synonym="C14orf102"
                     /note="UPF0614 protein C14orf102; protein NRDE2 homolog"
                     /codon_start=1
                     /product="nuclear exosome regulator NRDE2"
                     /protein_id="NP_060440.2"
                     /db_xref="CCDS:CCDS9890.1"
                     /db_xref="GeneID:55051"
                     /db_xref="HGNC:HGNC:20186"
                     /db_xref="MIM:618631"
                     /translation="MALFPAFAGLSEAPDGGSSRKELDWLSNPSFCVGSITSLSQQTE
                     AAPAHVSEGLPLTRSHLKSESSDESDTNKKLKQTSRKKKKEKKKKRKHQHHKKTKRKH
                     GPSSSSRSETDTDSEKDKPSRGVGGSKKESEEPNQGNNAAADTGHRFVWLEDIQAVTG
                     ETFRTDKKPDPANWEYKSLYRGDIARYKRKGDSCLGINPKKQCISWEGTSTEKKHSRK
                     QVERYFTKKSVGLMNIDGVAISSKTEPPSSEPISFIPVKDLEDAAPVTTWLNPLGIYD
                     QSTTHWLQGQGPPEQESKQPDAQPDSESAALKAKVEEFNRRVRENPRDTQLWMAFVAF
                     QDEVMKSPGLYAIEEGEQEKRKRSLKLILEKKLAILERAIESNQSSVDLKLAKLKLCT
                     EFWEPSTLVKEWQKLIFLHPNNTALWQKYLLFCQSQFSTFSISKIHSLYGKCLSTLSA
                     VKDGSILSHPALPGTEEAMFALFLQQCHFLRQAGHSEKAISLFQAMVDFTFFKPDSVK
                     DLPTKGQVEFFEPFWDSGEPRAGEKGARGWKAWMHQQERGGWVVINPDEDDDEPEEDD
                     QEIKDKTLPRWQIWLAAERSRDQRHWRPWRPDKTKKQTEEDCEDPERQVLFDDIGQSL
                     IRLSSHDLQFQLVEAFLQFLGVPSGFTPPASCLYLAMDENSIFDNGLYDEKPLTFFNP
                     LFSGASCVGRMDRLGYPRWTRGQNREGEEFIRNVFHLVMPLFSGKEKSQLCFSWLQYE
                     IAKVIWCLHTKNKKRLKSQGKNCKKLAKNLLKEPENCNNFCLWKQYAHLEWLLGNTED
                     ARKVFDTALGMAGSRELKDSDLCELSLLYAELEVELSPEVRRAATARAVHILTKLTES
                     SPYGPYTGQVLAVHILKARKAYEHALQDCLGDSCVSNPAPTDSCSRLISLAKCFMLFQ
                     YLTIGIDAAVQIYEQVFAKLNSSVFPEGSGEGDSASSQSWTSVLEAITLMHTSLLRFH
                     MKVSVYPLAPLREALSQALKLYPGNQVLWRSYVQIQNKSHSASKTRRFFDTITRSAKP
                     LEPWLFAIEAEKLRKRLVETVQRLDGREIHATIPETGLMHRIQALFENAMRSDSGSQC
                     PLLWRMYLNFLVSLGNKERSKGVFYKALQNCPWAKVLYLDAVEYFPDEMQEILDLMTE
                     KELRVRLPLEELELLLED"
     exon            102..210
                     /gene="NRDE2"
                     /gene_synonym="C14orf102"
                     /inference="alignment:Splign:2.1.0"
     exon            211..444
                     /gene="NRDE2"
                     /gene_synonym="C14orf102"
                     /inference="alignment:Splign:2.1.0"
     exon            445..594
                     /gene="NRDE2"
                     /gene_synonym="C14orf102"
                     /inference="alignment:Splign:2.1.0"
     exon            595..1042
                     /gene="NRDE2"
                     /gene_synonym="C14orf102"
                     /inference="alignment:Splign:2.1.0"
     exon            1043..1448
                     /gene="NRDE2"
                     /gene_synonym="C14orf102"
                     /inference="alignment:Splign:2.1.0"
     exon            1449..1582
                     /gene="NRDE2"
                     /gene_synonym="C14orf102"
                     /inference="alignment:Splign:2.1.0"
     exon            1583..1703
                     /gene="NRDE2"
                     /gene_synonym="C14orf102"
                     /inference="alignment:Splign:2.1.0"
     exon            1704..1879
                     /gene="NRDE2"
                     /gene_synonym="C14orf102"
                     /inference="alignment:Splign:2.1.0"
     exon            1880..2266
                     /gene="NRDE2"
                     /gene_synonym="C14orf102"
                     /inference="alignment:Splign:2.1.0"
     exon            2267..3195
                     /gene="NRDE2"
                     /gene_synonym="C14orf102"
                     /inference="alignment:Splign:2.1.0"
     exon            3196..3334
                     /gene="NRDE2"
                     /gene_synonym="C14orf102"
                     /inference="alignment:Splign:2.1.0"
     exon            3335..3406
                     /gene="NRDE2"
                     /gene_synonym="C14orf102"
                     /inference="alignment:Splign:2.1.0"
     exon            3407..14008
                     /gene="NRDE2"
                     /gene_synonym="C14orf102"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3789..3794
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NRDE2"
                     /gene_synonym="C14orf102"
                     /note="hexamer: ATTAAA"
     polyA_site      3810
                     /gene="NRDE2"
                     /gene_synonym="C14orf102"
                     /note="major polyA site"
     polyA_site      10807
                     /gene="NRDE2"
                     /gene_synonym="C14orf102"
     regulatory      13990..13995
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NRDE2"
                     /gene_synonym="C14orf102"
                     /note="hexamer: AATAAA"
     polyA_site      14008
                     /gene="NRDE2"
                     /gene_synonym="C14orf102"
ORIGIN      
        1 atccctatag agaagaacgg aggtacggcc tgtggtcatg gcgctgttcc cagcctttgc
       61 ggggcttagt gaggctcccg atggcgggag ctccaggaaa gagttagact ggctgagcaa
      121 cccaagcttt tgtgttggat ccataacgtc cctgagccaa caaactgaag cagctccagc
      181 ccatgtttct gaagggttac cgctgacaag gagtcatctg aaatcagagt cttcagatga
      241 aagtgacact aacaaaaagc tcaaacaaac aagtagaaaa aagaagaaag agaaaaagaa
      301 aaaaaggaag catcagcatc ataagaaaac aaagaggaag catgggccgt cgagtagcag
      361 caggtctgag acagacaccg attctgaaaa ggacaaacct tccagaggcg ttggaggcag
      421 taaaaaggaa tctgaggaac cgaatcaagg aaataatgct gcagctgata ctggacatcg
      481 ctttgtttgg cttgaggaca ttcaggctgt gacgggagaa accttcagaa cagataagaa
      541 accagatcct gcgaactggg agtacaagtc tctctaccga ggggatatag caagatacaa
      601 gaggaaagga gactcctgcc ttggcattaa ccctaagaag cagtgcatat cttgggaagg
      661 gacttccaca gagaagaagc attcacgcaa gcaggttgaa cgctatttta ctaagaagag
      721 tgtgggatta atgaacatcg atggagttgc cattagcagt aaaactgaac ctccctcatc
      781 tgagcccatc tcctttatac cagtgaagga cttggaagat gcggctcctg ttacaacctg
      841 gttgaatcct ctggggattt atgatcagtc aaccacacat tggctacaag gacagggtcc
      901 tccagagcag gaatcaaagc agccagacgc acagccagac agcgagagtg cggctctcaa
      961 ggccaaggtg gaggagttta acaggagggt gcgggagaat cctcgggata cgcagctgtg
     1021 gatggcattt gttgcttttc aggacgaggt catgaaaagt cctggcctgt atgccatcga
     1081 ggaaggagag caggaaaagc gaaagaggtc cctgaagctc attctggaga agaagctggc
     1141 cattctggag cgggccattg agagcaacca gagcagtgtg gatctgaaac tggccaagct
     1201 gaagctctgc acagagttct gggagccctc cactctggtc aaagagtggc agaaactgat
     1261 atttttgcat cccaataata cagccctttg gcagaaatac cttttatttt gccagagcca
     1321 gtttagtacc ttttcgatat caaaaattca cagtctttat ggaaaatgct tgagcacttt
     1381 gtctgctgtt aaggacggca gcatcttatc tcaccctgcg ttgcctggca cggaagaggc
     1441 catgtttgca ctctttcttc agcagtgcca ctttctgcgg caggctggcc actctgagaa
     1501 ggccatctca ttgttccagg ccatggtgga cttcaccttc ttcaaacccg acagcgtgaa
     1561 agatctgcct accaaaggac aggtggaatt ctttgaaccc ttttgggaca gtggagagcc
     1621 ccgggctggg gagaagggag cccgaggctg gaaggcgtgg atgcaccagc aggaacgagg
     1681 tggctgggtg gtcatcaacc cagatgagga tgacgatgaa ccagaagagg atgaccagga
     1741 aataaaagat aagactctgc ccaggtggca gatctggctt gctgctgagc gttcccgtga
     1801 ccagaggcac tggcggccct ggcgccctga taagaccaag aagcaaaccg aggaagactg
     1861 tgaggatccc gagagacagg tgttgtttga tgatattggg caatctttga tcagactttc
     1921 cagccatgat cttcagttcc agctggtgga ggccttcctg cagttcttgg gtgtgccttc
     1981 tggctttact cctccagcct cctgtcttta tctggccatg gatgagaaca gcatctttga
     2041 taatggactt tatgatgaaa agcccttgac ttttttcaac cctttgtttt ctggggctag
     2101 ctgtgttggc cgcatggata ggttgggcta tcctcgctgg accaggggtc agaaccgaga
     2161 gggcgaggag ttcatccgca atgtcttcca ccttgtcatg cctttatttt caggcaaaga
     2221 gaagtcccag ctctgcttct cctggttaca gtatgagatt gcaaaggtca tttggtgcct
     2281 gcacactaaa aacaagaaga gattaaagtc tcaagggaag aactgcaaaa aactagccaa
     2341 gaatctcctt aaggagccag aaaactgcaa caacttttgc ctgtggaagc agtatgcaca
     2401 tctggagtgg ttgcttggca acacggagga tgccagaaaa gtttttgaca cagcacttgg
     2461 catggcagga agcagagaac tgaaagactc tgacctctgt gagctcagtc tgctctatgc
     2521 tgagctggag gtggagctgt cgccagaagt gagaagggct gccacagctc gagctgttca
     2581 catattaacc aagctgactg agagcagccc ctatgggccc tacactggac aggtgttggc
     2641 tgttcacatt ttgaaagcgc gaaaggctta tgagcacgca ctgcaggact gtttgggtga
     2701 cagctgtgtc tccaatccag ctcccaccga ttcctgtagc cgcctaatta gcctggctaa
     2761 atgcttcatg ctcttccagt atttgaccat agggattgat gctgctgtgc agatatacga
     2821 acaggtgttt gcaaaactga acagttctgt tttcccagaa ggctctggcg agggggacag
     2881 tgccagctcc cagagttgga ccagtgttct cgaagccatc acactgatgc acacgagcct
     2941 gctgagattc cacatgaaag tgagtgttta cccgctggcc cctctgcgag aggcactctc
     3001 acaggcttta aagttgtatc caggcaacca ggttctttgg aggtcctatg tacagattca
     3061 gaataagtcc cacagtgcca gcaaaaccag gagatttttt gacacaatca ccaggtctgc
     3121 caaacccttg gagccttggt tgtttgcaat tgaagctgag aaactgagga agagactggt
     3181 ggaaactgtc cagaggttag acggtagaga gatccacgcc acaattcctg agaccggctt
     3241 aatgcatcgg atccaagccc tgtttgaaaa tgccatgcgc agcgacagtg gcagccagtg
     3301 ccccttgctg tggaggatgt atttgaactt tctggtttcc ttaggaaata aagaaagaag
     3361 caaaggtgta ttctacaaag cacttcagaa ttgcccttgg gcaaaggtgt tgtacctgga
     3421 cgccgtggag tatttccccg atgagatgca ggagatcctg gacctgatga ctgagaagga
     3481 gctccgggtg cgcctgccgc tggaggagct ggagctgctg ctggaggatt agagagcagc
     3541 gggaaaacgg gctgtgcctg cgaggccaag ttgcccaccc tgcggagcta ggaggcgcga
     3601 gcagagaacg tgtgtgttag gagaactcgg cttttgaaat gttctttctc gatagtaata
     3661 atgtgggctg ccagcctctc acatcttgca cactttttgg gtgtgtaaat gacacaaaag
     3721 ttatttacat attatatatg tgaatatgtg tatatatgta catagccaga gagtcatgcc
     3781 acgtggtcat taaaccgatg atgattgagg cgtggcagcg tccttgctca ttctctccct
     3841 gtgcgcagcc agcactggcc cccgcacggt gaatgtgttc cctccgctgg tgtcgtggtg
     3901 gtcctgccag gccctctctg agataaaggt ctggtttttc agcactagga tgtcttcccc
     3961 aggctggtga gcaggagatt ttgctcttgt gttgagaccc gcagttaacg cttggtgttc
     4021 tggcctgcac gcagtgtgtg gcaagttccc tgagtttacc tcggcggcga tgcctccgcc
     4081 tggagctgga ggggcagcgt gccctggggc tgctgaaaaa ctcaggcctg tggagtcaga
     4141 acccgctctt cagcaggtcc caggccactt gagtgtggaa ggctgcccgg ggcactggca
     4201 gcctctgggg gaagcctgct cccaagccgt ctaggagccg agcacgctgt accgccagcc
     4261 ccagccctgc ctgcatgagg ggcccggcac gcttgtgcag caacgctgct tacactatga
     4321 aggccaaggc tggcctggtt ctggtgttgc attcgagggc tgtgaacaga atgaaatgtc
     4381 aacatccatt tttcagactt gttctgcctc cgaagagatt tttagtgctg agtgattttc
     4441 actattaaca tatggctgga aggaatggtt ttcttatctt ctccgagctc tagactggct
     4501 agcttctctc tggctttttt tgttttgttt tcctctcttt agaattgctg tatacttgta
     4561 tctcctcagc acatttagaa aagccagtgt ataattttgg ctggcaactg acggacgcag
     4621 cgggcagcag caggcagggc agcgcacgag agcacctccc agggcacggt gggaaactgg
     4681 ccagcccagc cccactactg ccctctcctg ctcgtcccca gtccctgagc tacaggtggc
     4741 tgtaggcaga agcccaccgg gtgttcctta gcgacagaca ggtcgtcagc tgctcccctt
     4801 gatgcctttt cacagaaaca accctcaact tgaggaagtt gagagctcca ctccgaggtg
     4861 gacttgctcc ccctccctga gagctaactg gcttgaactg tcatgagttc ctggctgccc
     4921 acaaagaggg accttctcca cccttgtcct ctgcgtgggg cagacatcag ccagacatgt
     4981 caccgacctg attgggggca gggagtagga agtccaggac caaaccatgc tccccatgac
     5041 agcagctggc gcctcagtcc ccatctgact catgtctctt ctcatctggt tcagaactta
     5101 gaagggcgtg gcctctgatc agctggttcc ctccaaaccc cagtggcaac ctgtcccttg
     5161 agagccagga atggctctta gcagtgggca gtgagccccc gagaggataa agcggctgct
     5221 cgaacagatt gcgagcacat tacttgctgc atgggaggag gtttaaaaca aatcaaagat
     5281 tccttgaggg cattttacag aaaacaagat gtcaagagca tcactgttgt tatttaaaag
     5341 cccatttgag gccaggcgcg gtggctcacg tctgtaatcc cagcactttg ggaggccgag
     5401 gcggacggat cacgaggtca ggagatcgag accatcctgg ctaacatggt gaaacccccg
     5461 tctctactaa aaatacaaaa aattagccgg gcgtggtggc tggcgcctgt agtcccagct
     5521 actcggaagg ctaaggcagg agaatggcgt gaacctggga ggcagagctt gcagtgagcc
     5581 aagatcgcgc cactgcactc cagcctgggc gacacagcaa gactccgtct cgaaaaaaaa
     5641 aaaaaaaaaa cagcccgttt gagattgctg acatgaactt ctgaaagatg actacgtgcc
     5701 tcccaagact aaccgtcagc ttggagggct gagaaggggg ttcccagctg gagtcaaaca
     5761 gtggctcatg tgtttcatca ggaacgctcg tcatttcccc gaaggagtca ctgatcacat
     5821 cagaccacag gggtcggccc acctcgggtt tgggacagtg ttctttcctg gaatgaagca
     5881 gtggcaatgc ctctggcaca ggattgctgg gcctgcagga ggccaggagg cacctctgtg
     5941 ctgtgttccc agtaggagga ggcagctact acttgtaagg cacacatatc attttgcatt
     6001 ggaaaagtga agataggctt gctgcagagg gagtgatggg agagttcacc ttaaattgtt
     6061 ctactgagga agggctccaa agagccagtg ggttttccgc aggcagacgg ggttctccct
     6121 gtccggctca ccagtgcagg aagccaggga agaaattact gtgggaattc tgttatttca
     6181 caagcacttc atacctgcat ccctgcactt ggcaccgcag gcctcagtga ccagctgccg
     6241 aaacggtaat ttcagcattt tggtcagtaa gcaaactgga gaagcagctg gggtggggcg
     6301 cagttcagcc cgctaccctg acactctagg ccctggcatt tgctttagct gcttttgtag
     6361 aattgacatg agggagactg tccctctaga ggaaaacgct cagctattac caatgaggaa
     6421 aaggcaatac tgatgtgctg gggttttttg ttttttttaa tctcaaactt aggaaaagat
     6481 gcaagtgcgt tacaaagaat tttgtttcct gaaccgtgtg agtcagttgc tgagagtctg
     6541 ctacccgccc acgatacaga cactgaacag gaagtcagca tggacgcagt actcctagca
     6601 ggtcctcagg cccagtcatg tcctctagga aaaggatcca gttcaggagc aggagctgca
     6661 cttggttgtc atctctttgg tctccttctg attggaagct ttttgacttc caagacgtgg
     6721 atacttttga agtgttctga atttgtagaa tgttcctcag tttgtgtttg cctggtgttt
     6781 cctcagcact ggattcaggc taagcatctt tggcaggagt gtgacagcac tgacactctt
     6841 gggtggcacg tactttcaaa gtgcccatta ctgatgatgt gcatttgacc acttggtgaa
     6901 gacggtgtct gctgggcatc tccagtgtga ctttcccttt ctaactgaac agtgttttgt
     6961 ggcagactct caaaatctgt aaatacccct tcctcagcaa actttcagat caattcatat
     7021 gtattggggt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgtgtgt
     7081 agttccctat ttaaagggct atactgtatt ccattaatag taactgtttg ttaggatgct
     7141 cagattgtcc cgagtttggc cagtggaagc cccttcacat aggcttcggt gtccttagga
     7201 catgtcctta tcattccttg agcactccct tgctttctgg caaaacagac tttgggttca
     7261 tcctgtcctt tccctgccta gccccggaat cagccctcac tccttttagt ggagaataat
     7321 acttttagca gcaaaggtct gggtctaggg gtgctcactg cagttggggt gtcactgttc
     7381 ccaggtgctc ccagtggaca gaggtcgttc ttgttttctt ctttctgtgt ctaactcctc
     7441 agagaaacct ggctcccact gtcctgttta cctgcctgac tgcctccata cataagcagc
     7501 ctccctcccc tgccaccacc ccgtccctgc agatgccttc ctcacccact tgggctctca
     7561 tactgcacat caggctgccc acactgactc cccctatcgg accaccccag ccctgcacat
     7621 acaccccaca cggatgccag cctcaccctg cccatgctga gtccccgtgc tgtggtgttc
     7681 ctccacatgg acagctccac accctacttg ggctgacacc cgccctcccc acaagcaccc
     7741 cgtccttacc ctgcactggg ccacccccac gtgtggatgt cctctgtggt aggcagagtg
     7801 gcgcccctaa agatggccac accctaagcc ctggaacgtg tgaatgttac atggcaaaag
     7861 gaactttgca gataaaaatt aagaagattg taaactttaa aacagggaaa ttatcctgga
     7921 tcatctgcat gggtccaatg caatcacaag gatccataag aatagaaaag gcaggcagaa
     7981 agaagggtcg gtgggagaga gggactgggc ctgctgctgc tggtggaaaa tgaagtgcca
     8041 cgagtcagga gggagggtgg ccttcacaga ctggcaaagg cctcagctgg cagccagcaa
     8101 ggaaatgctg acctcagctg gcccacaact ccaagaaatt aaattctgcc aacaataatc
     8161 ctcagcaagg aaacggattc tcccttagag acgccagagg gaacggtcct gccaacagca
     8221 agactttagc ctggagacac tcgtgttgga cttctgacct acagaactga gataaaattg
     8281 tggtttttat ttatttattt atttattttt gagacggagt cttgctctgt cgcccaggct
     8341 ggagtgcagt gacgggatct tggctcactg caacctccgc ctcctgggtt caagcgattg
     8401 tcctgcctca gcctcctgag tagctgggac tacaggcacc tgccaccacg cacagctaat
     8461 ttttgtattt ttagtaaaga tggggtttca ccatgttggc caggctggcc tcaaacttct
     8521 gaccttgtga tccgcctgcc tcagcttccc aaagtgctgg gattacaggc gtgagccacc
     8581 gtgcctggcc aaattgtgct gtttttaaac aaagcctgtg gtaacttgtt atagtattaa
     8641 cagaaaacta atacacactc ctcattcagt gtgggcccta caacccacac caggccacct
     8701 gtcaagggaa cgcttacctt gatctgtccc acttaatagc tttaggcctg atttgttcag
     8761 aaagagaaat gacggaaata gacggagaaa gggaagggga agttctgaca tgtttctgaa
     8821 caccttgaaa gggatgagat ttctgagccc aagggctcag aagggctttc aagatatctc
     8881 taggaatcct cagtctcttg ggaacaccag tagttatttt ctgccctcag agtgctaaga
     8941 acatgcatgg tccacaaaac ttccaaaaag gaaagctgta cttcctttgg aagcaatagc
     9001 cacattaacg cagcctttat tatttaaaca acgaatatcc ctggctctgt ggtagtgacc
     9061 atttcccact gtaattaccg ttgaacgcca gctctggggg tgtgatgtgg cgaacgcata
     9121 gtgaggtcca cactccttgg gaccgcagcc tgagttcctg tccaaaggct tccctgtagc
     9181 tgggtccacc cacaggctgc tgttgagcgg taggtgtgtg tggagactgc agaaacagga
     9241 agtgtttgtg aaagagcacg ctttattggg aagcagactg ctgcacagtg accaacaggc
     9301 cacccaatgg gcacttacac atcgtactcc aaaagacaca ggatgttttg ctaataaaaa
     9361 tcagtgggaa cagggattcc tctgggcaac tcccccaacc tcatcccttt cagggattga
     9421 gaaatctccc aaccattttc ctgatgacag ccatggttca ttagagatac agcccctcag
     9481 gggtgccttc ctgtttctta taaagaacat tttctttaga ttttttgaag tcttcatttg
     9541 ttactttcat tctacgttct cttaaggcca tcagaccagc ttctgtacag attgcctgta
     9601 agaaagaaga gtgtgttcag aaagtgacat actcattttt attctaacaa ggaattccaa
     9661 aaaacctatt ctgaggccag gcgcggtggc tcacacctgt aatcccagca ctctgggagg
     9721 ccgaggcagg tggatcgcct aaggtcagga gttcaagacc agcctggcca acacggtgaa
     9781 accccatctc tactaaaaat acaaaaagtt agccagggac ggtggcaggc acctgtaatc
     9841 ctagctactc gggaggctga ggcaggagaa ttgcttgaac ccgggaggca gaggttacag
     9901 tgagccgaga tcgcgccact gcactctagc ctgagcaacg agcgaaactc tacctcaaaa
     9961 aaaaaaaaaa aaaaatctgt tctgaaataa agcatgagac acctagacta aacacaaatt
    10021 gcttatttgg gaaagatttg atttttaaaa aaaatgtttt aagcatccta ttttctgaaa
    10081 ggatgtgaag tggcttaaac tgggagttac tgtaactaag actgacccaa gggtcagggc
    10141 tgtgggttct agttttggct caggttctaa caagctatgt ggcttgtaca acaccacttt
    10201 tgttaaagag aataatattc ctaggaaata aacaaaatta tgccctgaaa cttagaggca
    10261 attgacagta tatgtctatt tcatttactt atagtcataa aagaacatgt attctggtta
    10321 gatactgtaa gtggcagtat ctatactatg gaataatact acagagtata gcatttattc
    10381 caatatattt aatttctcag taattactgg cccaaagtaa aagaaaacaa taacccatgt
    10441 tacatgggtt actgttattg ttttaggcct gatctgttta gaaaggaaag agaaatgaag
    10501 gaaatagata gagaaggaca gcaaacaaac atattatgtg ggttactgtt attgttttct
    10561 ttttcttttg gccagtaatt actgtggcaa aaacaagatg tggcaaaaaa cacaatcctg
    10621 gcacagaagt ttgtttggtg atggccatca ggttatttac ttcaaatggt tcgggtgaaa
    10681 agaaagtttt gtgtatagaa agagaaaaag caaacaggta aaatgttaac tcttggcaaa
    10741 tcactgtgct attgttgcaa cttttttgaa ggcttgaaaa ttgtcaaagt aagttcttat
    10801 cttaaaattt taagatcttt caatttttaa aaaagaagat agattttact tgccaacaga
    10861 gacatctagt tctctaggac taactgccaa tgatccgtgg gctcagggca ggtgctggcc
    10921 tggaaaggca ctgcttgtgc tgtccctacc taaccatggc atttatgccc aacgaaaacc
    10981 cagctctttc caggaaaagc aggtagaacc catattcaac gctgccagac cttgctgtaa
    11041 attctggtca gctgggagga aatgtcctaa ctcatgtaca tttgtcataa aaccaagaag
    11101 tataatctgg tacaaccttc cagaacacag attgggagat gaatgcagcg ggtgcttggt
    11161 ccccactctg cggctctggg gagagttctc ccaggacata cccttcccct gacctgggac
    11221 tgagcagcta catttgacac accttgagaa agtcacctgg gcctaaggct tagtaccagc
    11281 gactcagcct tgtggctcca ggtgatttag cgtcattcac tgcataaaat gatgcattaa
    11341 gaatgacttc aatatcaagc acatatacca tttggatgta atcgtaaacc aatatgatga
    11401 ttaaaataca gacacccacc tgtccaggcc aaccaccatc ccaagagcac atatagactc
    11461 tttcctgatt gacggccaca ttcccagaaa taagctgaca cggctaggct ctcaccttga
    11521 tgtcagcacc agagaggtca tctttagcca tgatcaggtc gtccagggtt acatcatcag
    11581 ccagcgtcat cctgcttgtg tgaatctgaa agatgcgctt cttcgtcttt tcatcaggca
    11641 gggggaactc aatcttcctg tcaatgcggc ctgcaggtac aaagagattt gaagtcacac
    11701 caacatccaa acccaggctc ggccatggct cagaggcctc agcccatctc cttacatacg
    11761 aagtttcagt cggacagaat tctgtgggaa ggatgtctaa attttaaaaa gtaaacatat
    11821 atttttgtag tttgtagatt tctacatttg aattctcttc atttaaaaga aactctctct
    11881 gctttggtac cttcagaaat aaatgaaagg agcaattcct gagtgaaact gggcagacct
    11941 attgtgttaa aaacattaca aaagacagca aacaaatcac tgtttcttct gatgctacct
    12001 gaatgtaaaa aggacaaatc ctaccatcac atgaattatt tcttccagaa aaagcagcaa
    12061 gttcttgagg ctaaaaaggg aatgttatgg aaaagaaaag acaagacata agactggaat
    12121 tcaaagggcc aaggtgcaca gaccatatca tgctctgttt taaccctcat ctcaagagga
    12181 cctatttttt tgtaaattat cctaagtttc actgcagtat ttgctttttt atgtatcatt
    12241 ttataggcct cccaaagcac acttaaacac aaatatataa acacattaat ctccatgatg
    12301 aaaccaaagc aaatttaacc tggtctgata agtgctggat ccaaagtttc tattcggttt
    12361 gtggccatga taactttcac atctccccta gaatcaaatc catccaactg gttcagcagt
    12421 tccaacattg ttcgctgaat ttctctctca ccaccagaat tggagtcata tctttggaaa
    12481 aaaaaaaagg gaaggaatga agactcaact gatcgcctgc tcaaggggaa ttacagcaaa
    12541 gacctcattg tgacaaaaca ccttaacaaa cattcctgtt tcaactcaca tggcactccc
    12601 tcaattatgc tcggaaaaaa taaattcagc accccttgcc atggtctgaa tgtatttccc
    12661 caaatttatg tgttgaaact taatccctac tgtgtggcta agagatgagg ccttttggaa
    12721 agggctaaag gcatgaggca gagccttata cagcatgagt tccttctaaa aagagaaaaa
    12781 ggccttatac agcatgtatt ccttataaaa aatgctgcag ggaactagtg taggcccttt
    12841 ttgctctttt gtttgtcccc tgagcatttg tctcctctgc agaataggtg ccatctcgga
    12901 agcagagagc agccctcact agacatcaat gccagtgcct tgatcttggg cttctcagcc
    12961 tccagaactg tgggaaatac atttctattc tttatacaaa taccagtcct aaggtatttt
    13021 gtttagcagc acaaatggac taagacgctc ccatcagcca agttacagat catatttttt
    13081 catagcagtt tctgtgtctg aaattgtatc atttattctg tttccatgtt tggtatctga
    13141 tccctacaac ctgtgagctc ctccagggca gagacttgtg ctcacaactg tgtccctaga
    13201 gtccagatgg tcctggatca gggcgggtgc tcaagacatg gttattcata actgaccaat
    13261 ctctccagac gttcacagtt actgggcagt tgttagctgg ctcctgggag agcagagcct
    13321 aaagcatgca tggaaggcca cgcacagcaa gaactgtggc catagggaga gccccctgag
    13381 attgctcatt ctcaagagaa gagctatgcg gtgttcccta ctaaacaagg caaaataagt
    13441 atgagaaagt ctaccttttt gtcccaatgg cgtcaatttc atcaataaac acgatggacg
    13501 gtgcatgttc ttcagcaact cggaacaatt cccgtacgag tttgggccca tcacctaggt
    13561 acttctgaat aagttcagag ccaaccactc tcaagaaagt ggctgaggtt tggtttgcta
    13621 ctgctttggc taacaaggtt ttacctattt tggatgaaaa agataaaaaa aaaaaagaca
    13681 ccttaagtgc cattttaact taaaaactcg tatcattacc accttaaatg gacaccaaaa
    13741 acaggcgggc cctcatgcta aggcatggca ccaattcaag tgtcacgaca ttccattctc
    13801 ctaagtagat aagaggacat ttagaaaaat ctactaacat gtttggatta tccttagcta
    13861 ttttgctgaa cttatgataa cgtcagaaaa tactttttac ttggttattt tccaaccttg
    13921 acccagtttc ccctgaaaag ctgcgaacta taattacctg tacgaagaat tgcttcattc
    13981 cagtaatgaa ataaatctca tttttttc
//
