LOCUS       NM_198060               5613 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens nebulin related anchoring protein (NRAP), transcript
            variant 2, mRNA.
ACCESSION   NM_198060
VERSION     NM_198060.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5613)
  AUTHORS   Koskenvuo,J.W., Saarinen,I., Ahonen,S., Tommiska,J., Weckstrom,S.,
            Seppala,E.H., Tuupanen,S., Kangas-Kontio,T., Schleit,J., Helio,K.,
            Hathaway,J., Gummesson,A., Dahlberg,P., Ojala,T.H., Vepsalainen,V.,
            Kytola,V., Muona,M., Sistonen,J., Salmenpera,P., Gentile,M.,
            Paananen,J., Myllykangas,S., Alastalo,T.P. and Helio,T.
  TITLE     Biallelic loss-of-function in NRAP is a cause of recessive dilated
            cardiomyopathy
  JOURNAL   PLoS One 16 (2), e0245681 (2021)
   PUBMED   33534821
  REMARK    GeneRIF: Biallelic loss-of-function in NRAP is a cause of recessive
            dilated cardiomyopathy.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5613)
  AUTHORS   Jirka,C., Pak,J.H., Grosgogeat,C.A., Marchetii,M.M. and Gupta,V.A.
  TITLE     Dysregulation of NRAP degradation by KLHL41 contributes to
            pathophysiology in nemaline myopathy
  JOURNAL   Hum Mol Genet 28 (15), 2549-2560 (2019)
   PUBMED   30986853
REFERENCE   3  (bases 1 to 5613)
  AUTHORS   Truszkowska,G.T., Bilinska,Z.T., Muchowicz,A., Pollak,A.,
            Biernacka,A., Kozar-Kaminska,K., Stawinski,P., Gasperowicz,P.,
            Kosinska,J., Zielinski,T. and Ploski,R.
  TITLE     Homozygous truncating mutation in NRAP gene identified by whole
            exome sequencing in a patient with dilated cardiomyopathy
  JOURNAL   Sci Rep 7 (1), 3362 (2017)
   PUBMED   28611399
  REMARK    GeneRIF: Loss-of-function mutation in NRAP gene is associated with
            dilated cardiomyopathy.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5613)
  AUTHORS   Blandin,G., Marchand,S., Charton,K., Daniele,N., Gicquel,E.,
            Boucheteil,J.B., Bentaib,A., Barrault,L., Stockholm,D., Bartoli,M.
            and Richard,I.
  TITLE     A human skeletal muscle interactome centered on proteins involved
            in muscular dystrophies: LGMD interactome
  JOURNAL   Skelet Muscle 3 (1), 3 (2013)
   PUBMED   23414517
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5613)
  AUTHORS   Chen,X., Li,X., Wang,P., Liu,Y., Zhang,Z., Zhao,G., Xu,H., Zhu,J.,
            Qin,X., Chen,S., Hu,L. and Kong,X.
  TITLE     Novel association strategy with copy number variation for
            identifying new risk Loci of human diseases
  JOURNAL   PLoS One 5 (8), e12185 (2010)
   PUBMED   20808825
  REMARK    GeneRIF: Observational study and genome-wide association study of
            gene-disease association. (HuGE Navigator)
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5613)
  AUTHORS   Mohiddin,S.A., Lu,S., Cardoso,J.P., Carroll,S., Jha,S., Horowits,R.
            and Fananapazir,L.
  TITLE     Genomic organization, alternative splicing, and expression of human
            and mouse N-RAP, a nebulin-related LIM protein of striated muscle
  JOURNAL   Cell Motil Cytoskeleton 55 (3), 200-212 (2003)
   PUBMED   12789664
  REMARK    GeneRIF: Highly conserved and exclusively expressed in cardiac and
            skeletal muscle.  Candidate cause for cardiac and skeletal
            myopathies
REFERENCE   7  (bases 1 to 5613)
  AUTHORS   Luo,G., Herrera,A.H. and Horowits,R.
  TITLE     Molecular interactions of N-RAP, a nebulin-related protein of
            striated muscle myotendon junctions and intercalated disks
  JOURNAL   Biochemistry 38 (19), 6135-6143 (1999)
   PUBMED   10320340
REFERENCE   8  (bases 1 to 5613)
  AUTHORS   Luo,G., Leroy,E., Kozak,C.A., Polymeropoulos,M.H. and Horowits,R.
  TITLE     Mapping of the gene (NRAP) encoding N-RAP in the mouse and human
            genomes
  JOURNAL   Genomics 45 (1), 229-232 (1997)
   PUBMED   9339382
REFERENCE   9  (bases 1 to 5613)
  AUTHORS   Luo,G., Zhang,J.Q., Nguyen,T.P., Herrera,A.H., Paterson,B. and
            Horowits,R.
  TITLE     Complete cDNA sequence and tissue localization of N-RAP, a novel
            nebulin-related protein of striated muscle
  JOURNAL   Cell Motil Cytoskeleton 38 (1), 75-90 (1997)
   PUBMED   9295142
REFERENCE   10 (bases 1 to 5613)
  AUTHORS   Lanfranchi,G., Muraro,T., Caldara,F., Pacchioni,B., Pallavicini,A.,
            Pandolfo,D., Toppo,S., Trevisan,S., Scarso,S. and Valle,G.
  TITLE     Identification of 4370 expressed sequence tags from a
            3'-end-specific cDNA library of human skeletal muscle by DNA
            sequencing and filter hybridization
  JOURNAL   Genome Res 6 (1), 35-42 (1996)
   PUBMED   8681137
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA564310.1, AY177620.1, AL390197.19 and F30818.1.
            
            On Jun 2, 2019 this sequence version replaced NM_198060.3.
            
            Transcript Variant: This variant (2) includes an alternate exon in
            the coding region, but maintains the reading frame, compared to
            variant 1. The encoded isoform (2, also known as isoform S) is
            longer than isoform 1.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BX647658.1, AL831980.2 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1968540 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000359988.4/ ENSP00000353078.3
            RefSeq Select criteria :: based on manual assertion, conservation
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-18                DA564310.1         33-50
            19-458              AY177620.1         1-440
            459-459             AL390197.19        171807-171807       c
            460-1643            AY177620.1         442-1625
            1644-1644           AL390197.19        148431-148431       c
            1645-3705           AY177620.1         1627-3687
            3706-3726           AL390197.19        124772-124792       c
            3727-5358           AY177620.1         3709-5340
            5359-5503           F30818.1           227-371
            5504-5613           AL390197.19        102991-103100       c
FEATURES             Location/Qualifiers
     source          1..5613
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q25.3"
     gene            1..5613
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /note="nebulin related anchoring protein"
                     /db_xref="GeneID:4892"
                     /db_xref="HGNC:HGNC:7988"
                     /db_xref="MIM:602873"
     exon            1..231
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     CDS             160..5352
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /note="isoform 2 is encoded by transcript variant 2"
                     /codon_start=1
                     /product="nebulin-related-anchoring protein isoform 2"
                     /protein_id="NP_932326.2"
                     /db_xref="CCDS:CCDS7579.1"
                     /db_xref="GeneID:4892"
                     /db_xref="HGNC:HGNC:7988"
                     /db_xref="MIM:602873"
                     /translation="MNVQPCSRCGYGVYPAEKISCIDQIWHKACFHCEVCKMMLSVNN
                     FVSHQKKPYCHAHNPKNNTFTSVYHTPLNLNVRTFPEAISGIHDQEDGEQCKSVFHWD
                     MKSKDKEGAPNRQPLANERAYWTGYGEGNAWCPGALPDPEIVRMVEARKSLGEEYTED
                     YEQPRGKGSFPAMITPAYQRAKKANQLASQVEYKRGHDERISRFSTVVDTPELLRSKA
                     GAQLQSDVRYTEDYEQQRGKGSFPAMITPAYQIAKRANELASDVRYHQQYQKEMRGMA
                     GPAIGAEGILTRECADQYGQGYPEEYEEHRGKGSFPAMITPAYQNAKKAHELASDIKY
                     RQDFNKMKGAAHYHSLPAQDNLVLKQAQSVNKLVSEVEYKKDLESSRGHSINYCETPQ
                     FRNVSKISKFTSDNKYKENYQNHMRGRYEGVGMDRRTLHAMKVGSLASNVAYKADYKH
                     DIVDYNYPATLTPSYQTAMKLVPLKDANYRQSIDKLKYSSVTDTPQIVQAKINAQQLS
                     HVNYRADYEKNKLNYTLPQDVPQLVKAKTNAKLFSEVKYKEGWEKTKGKGFEMKLDAM
                     SLLAAKASGELASNIKYKEEYEKTKGKAMGTADSRLLHSLQIAKMSSEVEYKKGFEES
                     KTRFHLPMDMVNIRHAKKAQTLASDLDYRKKLHEYTVLPEDMKTQWAKKAYGLQSELQ
                     YKADLAWMKGVGWLTEGSLNLEQAKKAGQLVSEKNYRQRVDELKFTSVTDSSQMEHAK
                     KSQELQSGVAYKAGNEQSVHQYTISKDEPLFLQARANAANLSEKLYKSSWENQKAKGF
                     ELRLDSLTFLAAKAKRDLASEVKYKEDYERSRGKLIGAKDVQGDSQMSHSLQMSKLQS
                     ELEYKKGFEDTKSQCHVSLDMVHLVHARKAQHLATDVGYKTAEHHFTALPTDMKVEWA
                     KKAYGLQSDNQYRADVKWMKGMGWVATGSLNVEQAKKAGELISEKKYRQHPDALKFTS
                     IKDTPEMVQARISYTQAVDRLYREQGENIKHHYTPTADLPEVLLAKLNAMNISETRYK
                     ESWSKLRDGGYKLRLDALPFQAAKASGEIISDYKYKEAFEKMKGQMLGSRSLEDDISL
                     AHSVYATSLQSDVNYKKGFEHSKAQFHLPLDMAALVHAKKAQTLASNQDYKHPLPQYT
                     SLAEDLRLSCAKKAHKLQSENLYRSDLNFMRGVACVIPGTLEIEGRKKASELISESKY
                     RQHPHSFKYTAVTDTPNLLHAKFSNQITNERLYKAAGEDARHEYTMTLGLPEFIRAKT
                     NAANLSDARYKESWRNLRAQGYKLTIEALPFQAARASGDIASDFLYRHDFVKERGKLI
                     GPQSVRDDPRIQHCRRMGQLQSELQYRRGATSSQAQFHLPMDMVHLVHAKNAQALASD
                     HDYRTQYHKFTALPEDLKMAWAKKAHALQSELRYKSDLIGMKGIGWLALRSPQMESAK
                     KAGELISETKYRKKPDSIKFTTVVDSPDLVHAKNSYMHCNERMYRSGDAESLHRYTLI
                     PDHPDFTRARLNALHLSDKVYRNSWEQTRAGSYDFRLDAIPFQTARASREIASDFRYK
                     EAFLRDRGLQIGYRSVDDDPRMKHFLNVGRLQSDNEYKKDFAKSRSQFHSSTDQPGLL
                     QAKRSQQLASDVHYRQPLPQPTCDPEQLGLRHAQKAHQLQSDVKYKSDLNLTRGVGWT
                     PPGSYKVEMARRAAELANARGLGLQGAYRGAEAVEAGDHQSGEVNPDATEILHVKKKK
                     ALLL"
     exon            232..326
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            327..414
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            415..519
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            520..624
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            625..729
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            730..834
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            835..942
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            943..1047
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1048..1152
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1153..1269
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1270..1374
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1375..1482
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1483..1587
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1588..1686
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1687..1791
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1792..1899
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            1900..2001
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2002..2199
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2200..2304
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2305..2403
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2404..2508
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2509..2616
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2617..2928
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            2929..3033
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3034..3132
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3133..3237
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3238..3345
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3346..3459
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3460..3657
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3658..3762
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3763..3861
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3862..3966
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            3967..4074
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            4075..4386
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            4387..4491
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            4492..4590
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            4591..4695
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            4696..4803
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            4804..5115
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            5116..5247
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     exon            5248..5613
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5474..5479
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /note="hexamer: ATTAAA"
     regulatory      5479..5484
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /note="hexamer: AATAAT"
     polyA_site      5497
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /note="major polyA site"
     polyA_site      5506
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
     regulatory      5585..5590
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
                     /note="hexamer: AATAAA"
     polyA_site      5613
                     /gene="NRAP"
                     /gene_synonym="N-RAP"
ORIGIN      
        1 agaaggtcag caaaggaaag tggaagttgg attctgaaag atcgaggtgc ccacaggaat
       61 tttatggtcg tcggattttg aagacttgaa ctagactggg ggttctcctt gcatttcttg
      121 cctgttgcct atctttgtcc tctctcttcc ggcttcgaga tgaatgtgca gccctgttct
      181 aggtgtgggt atggggttta tcctgccgag aagatcagct gtatagatca gatatggcat
      241 aaagcctgtt ttcactgtga agtttgcaag atgatgctgt ctgttaataa ctttgtgagt
      301 caccagaaaa agccgtactg tcacgcccat aaccctaaga acaacacttt caccagtgtc
      361 tatcacactc cattaaatct aaatgtgagg acatttccag aggccatcag tgggatccat
      421 gaccaagaag atggtgaaca gtgtaaatca gtttttcact gggacatgaa atccaaggat
      481 aaggaaggtg cacctaacag gcagccactg gcaaatgaga gagcctattg gactggatat
      541 ggggaaggga atgcttggtg cccaggagct ctgccagacc ccgaaattgt aaggatggtt
      601 gaggctcgaa agtctcttgg tgaggaatat acagaagact atgagcaacc caggggcaag
      661 gggagctttc cagccatgat cacacctgct tatcaaaggg ccaagaaagc caaccagctg
      721 gccagccaag tggagtataa gagagggcat gatgaacgca tctccaggtt ctccacggtg
      781 gtggatactc ctgagctgct acggagcaag gctggggcac agcttcaaag tgatgtgaga
      841 tacacagagg actatgaaca acaaagaggg aaaggcagtt tccctgcgat gatcacaccc
      901 gcctatcaga tagccaaaag agccaatgag ctggcaagtg atgtgaggta ccatcaacaa
      961 tatcaaaaag aaatgagggg aatggctggt ccagccattg gagctgaggg catcttgaca
     1021 agggaatgtg cagaccaata tggccagggt tacccggagg agtatgagga gcacagggga
     1081 aagggcagct tcccagctat gatcactcca gcatatcaga acgccaagaa agctcacgaa
     1141 ctcgctagtg acataaaata caggcaggac ttcaataaga tgaaaggcgc tgcacattat
     1201 cactcgcttc cagctcaaga caacttggtt ctcaaacagg ctcagagcgt aaacaaactc
     1261 gtgagtgagg tggagtataa gaaggatctg gaaagtagta gaggtcacag tatcaactac
     1321 tgtgaaacac ctcaattcag gaacgtgagc aagatctcaa aatttaccag tgataataaa
     1381 tataaagaaa actaccagaa ccacatgaga ggccgctatg aaggagttgg tatggacaga
     1441 cgcactctgc atgctatgaa agttggcagc ctggcaagca acgttgccta caaagctgat
     1501 tataaacatg atattgtcga ctacaactac ccagccactc tcacgccttc ctatcaaaca
     1561 gctatgaaac tggtgccctt gaaagatgcc aattataggc agagcatcga caagttgaag
     1621 tacagctcgg tgactgacac cccacagatt gttcaagcca aaatcaatgc ccagcagctg
     1681 agtcatgtga attaccgtgc tgactatgag aaaaataagt tgaattacac attgccccag
     1741 gatgttcctc agctggtgaa ggccaaaacc aatgccaaac tcttcagtga ggttaagtat
     1801 aaagaaggct gggagaagac aaaggggaaa ggatttgaga tgaagctgga tgccatgtct
     1861 ctgctggccg ccaaagcctc tggggagctt gctagcaata ttaaatataa agaagaatat
     1921 gaaaagacaa aaggcaaagc catgggaaca gccgactcta ggcttctgca ctccctgcag
     1981 attgctaaga tgagcagtga ggttgaatat aagaaaggct ttgaagagag taagacccgg
     2041 tttcacctgc ccatggatat ggtaaacatc aggcatgcta agaaggccca aactctcgcc
     2101 agtgacctgg actacaggaa gaaactgcat gaatacactg tgctgcctga agatatgaag
     2161 actcagtggg ccaagaaggc ctatgggctc cagagcgagc tgcagtacaa ggctgacctg
     2221 gcatggatga agggagttgg gtggctgaca gaggggagtc tcaacttgga acaagccaag
     2281 aaggctggac agctggtcag cgagaaaaac taccggcaga gggtcgatga gctgaagttc
     2341 accagcgtga ccgacagctc ccagatggag cacgccaaga agagccagga gctacagagc
     2401 ggggtggcct acaaggcagg aaatgagcag tctgtccatc agtataccat cagcaaagac
     2461 gagcctctct tcctgcaggc ccgagccaat gctgcaaatc tcagcgagaa actgtataag
     2521 agcagctggg aaaaccagaa agcaaaaggg tttgagctgc gtcttgattc cttgaccttc
     2581 ctggcagcca aagccaagag ggacctggct agcgaggtga agtacaagga ggattatgag
     2641 agatccagag ggaagctcat tggggcaaaa gatgtacagg gagattcgca aatgagccac
     2701 tcactgcaaa tgtccaagct gcagagtgag ctggagtaca agaagggatt cgaggacacc
     2761 aaatcccaat gccacgtctc cctggacatg gtccacctcg tgcatgcccg caaagctcag
     2821 catttagcca cagacgtagg ctacaagaca gcggaacatc actttacggc tttgcccaca
     2881 gacatgaagg tggaatgggc caagaaggct tatggcttac agagtgataa ccaatacagg
     2941 gcagatgtga agtggatgaa aggcatgggc tgggtcgcca ccgggtcatt aaatgtggag
     3001 caggcgaaga aggcaggaga actcattagc gagaagaagt accgtcagca tccagatgct
     3061 ttgaagttta ccagtattaa agacactccg gagatggtcc aggccagaat tagttatacc
     3121 caagcagtgg atagattata cagggaacaa ggagaaaaca taaagcatca ttacacaccg
     3181 actgctgacc tccctgaagt cctgctggcc aagctgaatg ccatgaatat cagtgagacg
     3241 cgttataagg aatcctggag caaacttcga gatggtggct ataaactgag gttggatgcc
     3301 cttccattcc aagcagcaaa ggcttctggt gaaatcataa gtgattacaa atacaaagag
     3361 gcatttgaga aaatgaaagg acagatgctt ggttcccgga gcttggaaga tgatatcagc
     3421 cttgcacatt cagtgtatgc gacctcactg cagagtgatg tgaattacaa gaaaggcttt
     3481 gaacactcaa aggcgcagtt ccatctgccg ttggacatgg ccgccctggt gcatgccaag
     3541 aaggctcaga ccctggccag caatcaggac tacaaacatc cactgcccca gtacacttcc
     3601 ttggcagaag acctgaggct gagctgtgcc aagaaagctc acaaattgca gagtgagaat
     3661 ttgtaccggt cagacctgaa ctttatgcga ggtgttgcat gtgtcattcc aggcacgtta
     3721 gagattgaag ggaggaagaa agcatcggaa ctcatcagtg agagtaaata tcggcagcat
     3781 ccccactcat tcaagtacac agctgtgaca gacactccca acctccttca tgcgaaattc
     3841 agcaaccaga taacgaatga gcgcctctat aaagcagctg gagaggatgc aagacacgag
     3901 tatacaatga ccctgggtct gcccgagttc atccgagcaa aaacgaatgc agccaacctg
     3961 agtgacgcaa gatacaaaga gtcctggcgt aatcttcgtg ctcaaggcta caagctgaca
     4021 atagaagcgc tccccttcca ggctgcccgg gcctctggag atatagccag tgattttctc
     4081 tacagacatg actttgtgaa ggagcgaggg aaactcatag ggccccagag tgtaagagac
     4141 gacccccgga tccagcactg ccggcgcatg ggccagctgc agagcgagct tcagtacagg
     4201 aggggggcga ccagcagcca agcccagttc catctgccca tggacatggt gcacctggtc
     4261 catgccaaga atgcccaggc tctggccagc gaccacgact acaggacaca gtatcacaag
     4321 ttcacagcac tgcccgagga cctgaagatg gcctgggcca agaaagccca tgccctgcag
     4381 agtgagttgc gctacaagtc agacctgatc ggcatgaagg gcataggatg gctggcgctg
     4441 agatccccac agatggagag tgcaaagaag gctggagaac tcatcagcga gaccaagtac
     4501 cgtaaaaaac cagacagtat caagttcacc acagtggttg actccccaga cctggttcat
     4561 gccaagaaca gctatatgca ctgcaatgag cgcatgtata gatctggaga tgcagaatcc
     4621 ctgcacagat acaccctgat ccccgaccat cccgatttca cccgagctcg cctcaatgcg
     4681 ctgcatctga gtgacaaagt ctacagaaac tcctgggagc agacccgggc tggcagttat
     4741 gacttcaggc tggatgccat ccccttccag actgcccggg catctaggga gatcgccagt
     4801 gatttccggt acaaagaggc tttcctgcgg gaccgaggcc tgcagatcgg gtaccgcagt
     4861 gtcgacgatg acccaaggat gaagcatttc ctcaacgttg gcaggctcca gagtgacaat
     4921 gagtacaaga aggactttgc caagagtcgg tcccagtttc acagcagcac agaccagccc
     4981 ggcctccttc aggccaagag gagccagcag ctggccagtg atgtgcacta caggcagccc
     5041 ctgccccagc ccacctgcga cccggagcag ctgggcctca ggcatgctca gaaggcccac
     5101 cagctgcaga gtgatgtcaa gtataaatca gacttgaacc tgaccagagg tgttggctgg
     5161 acccctcctg gctcctacaa agtggaaatg gctcggcggg ctgcggaact ggccaacgca
     5221 aggggcctgg gtctccaggg agcttaccgg ggggcagaag cagtggaggc tggagatcat
     5281 cagagtgggg aggtgaaccc agatgccact gagattctgc acgtcaaaaa gaagaaggcc
     5341 ctgctgttgt gagccatgtc caccctgatt cctgagaggc cagagaggaa gtttgttcac
     5401 cagagacagg cttcagatgg ctttgatttc ggcaagctgg aatggcccac cagcccatcc
     5461 cagatcttcc tttattaaaa taataactct gaaagcagat ggagaatgtt gtatttgtgg
     5521 tcctctctga tacctagacg tggatggctg attctgttga gtgcctcgag tgtctgggct
     5581 gaggaataaa gatgtgatgg tggttcccgc tga
//
