LOCUS       NM_001099456             751 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens neuropeptide W (NPW), mRNA.
ACCESSION   NM_001099456 XM_496111 XM_939689
VERSION     NM_001099456.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Ji,L., Zhu,H., Chen,H., Fan,W., Chen,J., Chen,J., Zhu,G. and
            Wang,J.
  TITLE     Modulation of CaV1.2 calcium channel by neuropeptide W regulates
            vascular myogenic tone via G protein-coupled receptor 7
  JOURNAL   J Hypertens 33 (12), 2431-2442 (2015)
   PUBMED   26536090
  REMARK    GeneRIF: our data highlight that NPW modulates the CaV1.2 currents
            via G protein-coupled receptor 7, and the presence of it influences
            arterial tone.
            Erratum:[J Hypertens. 2016 May;34(5):1023. doi:
            10.1097/01.hjh.0000482494.85475.78.. PMID: 27031934]
REFERENCE   2  (bases 1 to 751)
  AUTHORS   Li,H., Feinle-Bisset,C., Frisby,C., Kentish,S., Wittert,G.A. and
            Page,A.J.
  TITLE     Gastric neuropeptide W is regulated by meal-related nutrients
  JOURNAL   Peptides 62, 6-14 (2014)
   PUBMED   25270269
  REMARK    GeneRIF: these studies indicated an inconsistency between plasma
            and gastric NPW expression in response to nutrient ingestion,
            suggesting food induced gastric NPW expression may play a more
            important role locally
REFERENCE   3  (bases 1 to 751)
  AUTHORS   Green,B.R., Smith,M., White,K.L., White,H.S. and Bulaj,G.
  TITLE     Analgesic neuropeptide W suppresses seizures in the brain revealed
            by rational repositioning and peptide engineering
  JOURNAL   ACS Chem Neurosci 2 (1), 51-56 (2011)
   PUBMED   22826747
  REMARK    GeneRIF: This review of NPW suggests that study of effects of this
            peptide may reveal its role in regulating feeding and energy
            metabolism coupled to leptin levels in the brain.
            Review article
REFERENCE   4  (bases 1 to 751)
  AUTHORS   Luttrell,L.M.
  TITLE     Reviews in molecular biology and biotechnology: transmembrane
            signaling by G protein-coupled receptors
  JOURNAL   Mol Biotechnol 39 (3), 239-264 (2008)
   PUBMED   18240029
  REMARK    Review article
REFERENCE   5  (bases 1 to 751)
  AUTHORS   Lambert,N.A.
  TITLE     Dissociation of heterotrimeric g proteins in cells
  JOURNAL   Sci Signal 1 (25), re5 (2008)
   PUBMED   18577758
  REMARK    Review article
            Erratum:[Sci Signal. 2008 Jul 15;1(28):er3]
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 751)
  AUTHORS   Tanaka,H., Yoshida,T., Miyamoto,N., Motoike,T., Kurosu,H.,
            Shibata,K., Yamanaka,A., Williams,S.C., Richardson,J.A.,
            Tsujino,N., Garry,M.G., Lerner,M.R., King,D.S., O'Dowd,B.F.,
            Sakurai,T. and Yanagisawa,M.
  TITLE     Characterization of a family of endogenous neuropeptide ligands for
            the G protein-coupled receptors GPR7 and GPR8
  JOURNAL   Proc Natl Acad Sci U S A 100 (10), 6251-6256 (2003)
   PUBMED   12719537
  REMARK    GeneRIF: a non-AUG translation initiation site is used to express
            mouse prepro-Npw
REFERENCE   7  (bases 1 to 751)
  AUTHORS   Brezillon,S., Lannoy,V., Franssen,J.D., Le Poul,E., Dupriez,V.,
            Lucchetti,J., Detheux,M. and Parmentier,M.
  TITLE     Identification of natural ligands for the orphan G protein-coupled
            receptors GPR7 and GPR8
  JOURNAL   J Biol Chem 278 (2), 776-783 (2003)
   PUBMED   12401809
REFERENCE   8  (bases 1 to 751)
  AUTHORS   Shimomura,Y., Harada,M., Goto,M., Sugo,T., Matsumoto,Y., Abe,M.,
            Watanabe,T., Asami,T., Kitada,C., Mori,M., Onda,H. and Fujino,M.
  TITLE     Identification of neuropeptide W as the endogenous ligand for
            orphan G-protein-coupled receptors GPR7 and GPR8
  JOURNAL   J Biol Chem 277 (39), 35826-35832 (2002)
   PUBMED   12130646
  REMARK    GeneRIF: determined that non-AUG translation initiation is used to
            express human prepro-NPW
REFERENCE   9  (bases 1 to 751)
  AUTHORS   Fujii,R., Yoshida,H., Fukusumi,S., Habata,Y., Hosoya,M.,
            Kawamata,Y., Yano,T., Hinuma,S., Kitada,C., Asami,T., Mori,M.,
            Fujisawa,Y. and Fujino,M.
  TITLE     Identification of a neuropeptide modified with bromine as an
            endogenous ligand for GPR7
  JOURNAL   J Biol Chem 277 (37), 34010-34016 (2002)
   PUBMED   12118011
REFERENCE   10 (bases 1 to 751)
  AUTHORS   Itoh,H., Toyama,R., Kozasa,T., Tsukamoto,T., Matsuoka,M. and
            Kaziro,Y.
  TITLE     Presence of three distinct molecular species of Gi protein alpha
            subunit. Structure of rat cDNAs and human genomic DNAs
  JOURNAL   J Biol Chem 263 (14), 6656-6664 (1988)
   PUBMED   2834384
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AB084276.1 and BM985063.1.
            
            On Nov 23, 2018 this sequence version replaced NM_001099456.2.
            
            Summary: The product of this gene is processed into 23- and
            30-amino acid neuropeptides that bind and activate two G-protein
            coupled receptors in the central nervous system. The neuropeptides
            have been shown to enhance cortisol secretion from adrenal cells
            through the adenylate cyclase/protein kinase A signaling cascade.
            The preproprotein is translated using a non-AUG initiation codon
            that is inferred from analyses of the mouse ortholog. [provided by
            RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BM985063.1, SRR1163657.479299.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA1968189
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match       :: ENST00000566435.4/ ENSP00000456974.3
            non-AUG initiation codon :: PMID: 12719537, 12130646
            RefSeq Select criteria   :: based on single protein-coding
                                        transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-674               AB084276.1         46-719
            675-751             BM985063.1         18-94               c
FEATURES             Location/Qualifiers
     source          1..751
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16p13.3"
     gene            1..751
                     /gene="NPW"
                     /gene_synonym="L8; L8C; PPL8; PPNPW"
                     /note="neuropeptide W"
                     /db_xref="GeneID:283869"
                     /db_xref="HGNC:HGNC:30509"
                     /db_xref="MIM:607997"
     exon            1..528
                     /gene="NPW"
                     /gene_synonym="L8; L8C; PPL8; PPNPW"
                     /inference="alignment:Splign:2.1.0"
     CDS             118..615
                     /gene="NPW"
                     /gene_synonym="L8; L8C; PPL8; PPNPW"
                     /note="non-AUG (CUG) translation initiation codon;
                     preproprotein L8; prepro-neuropeptide W"
                     /codon_start=1
                     /product="neuropeptide W preproprotein"
                     /protein_id="NP_001092926.2"
                     /db_xref="CCDS:CCDS42102.1"
                     /db_xref="GeneID:283869"
                     /db_xref="HGNC:HGNC:30509"
                     /db_xref="MIM:607997"
                     /translation="MAWRPGERGAPASRPRLALLLLLLLLPLPSGAWYKHVASPRYHT
                     VGRAAGLLMGLRRSPYLWRRALRAAAGPLARDTLSPEPAAREAPLLLPSWVQELWETR
                     RRSSQAGIPVRAPRSPRAPEPALEPESLDFSGAGQRLRRDVSRPAVDPAANRLGLPCL
                     APGPF"
     exon            529..751
                     /gene="NPW"
                     /gene_synonym="L8; L8C; PPL8; PPNPW"
                     /inference="alignment:Splign:2.1.0"
     regulatory      715..720
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NPW"
                     /gene_synonym="L8; L8C; PPL8; PPNPW"
                     /note="hexamer: AATAAA"
     polyA_site      751
                     /gene="NPW"
                     /gene_synonym="L8; L8C; PPL8; PPNPW"
                     /note="major polyA site"
ORIGIN      
        1 ggctcgcctc cagcctcctg cgctccggta cctgggcgtc ccaactccac tgcgcgccca
       61 aacccagccg agccggttcg tggcccgccc cgccgggcgg ccgtcgacgc gagcgccctg
      121 gcgtggcgcc caggggagcg gggggctccc gcgagccggc cgcggctggc actgctgctg
      181 cttctgctcc tgctgccgct gccctccggc gcgtggtaca agcacgtggc gagtccccgc
      241 taccacacgg tgggccgcgc cgctggcctg ctcatggggc tgcgtcgctc accctatctg
      301 tggcgccgcg cgctgcgcgc ggccgccggg cccctggcca gggacaccct ctcccccgaa
      361 cccgcagccc gcgaggctcc tctcctgctg ccctcgtggg ttcaggagct gtgggagacg
      421 cgacgcagga gctcccaggc agggatcccc gtccgtgcgc cccggagccc gcgcgcccca
      481 gagcctgcgc tggaaccgga gtccctggac ttcagcggag ctggccagag acttcggaga
      541 gacgtctccc gcccagcggt ggaccccgca gcaaaccgcc ttggcctgcc ctgcctggcc
      601 cccggaccgt tctgacagcg tcccccgccc gcccgtggcg cctccgcgcc tgacccagga
      661 ggagtggccg cgcgcttcca ggagccgctc atagaccccg cctgccgtcc ggtcaataaa
      721 atccgcctga ctcctgcgcc cccgcatgcg a
//
