LOCUS       NM_002520               1320 bp    mRNA    linear   PRI 20-FEB-2025
DEFINITION  Homo sapiens nucleophosmin 1 (NPM1), transcript variant 1, mRNA.
ACCESSION   NM_002520
VERSION     NM_002520.7
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1320)
  AUTHORS   Khoroshkin,M., Buyan,A., Dodel,M., Navickas,A., Yu,J., Trejo,F.,
            Doty,A., Baratam,R., Zhou,S., Lee,S.B., Joshi,T., Garcia,K.,
            Choi,B., Miglani,S., Subramanyam,V., Modi,H., Carpenter,C.,
            Markett,D., Corces,M.R., Mardakheh,F.K., Kulakovskiy,I.V. and
            Goodarzi,H.
  TITLE     Systematic identification of post-transcriptional regulatory
            modules
  JOURNAL   Nat Commun 15 (1), 7872 (2024)
   PUBMED   39251607
  REMARK    Erratum:[Nat Commun. 2024 Oct 28;15(1):9277. doi:
            10.1038/s41467-024-52903-4.. PMID: 39468017]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1320)
  AUTHORS   Zhang,S., Zhang,Y., Duan,X., Wang,B. and Zhan,Z.
  TITLE     Targeting NPM1 Epigenetically Promotes Postinfarction Cardiac
            Repair by Reprogramming Reparative Macrophage Metabolism
  JOURNAL   Circulation 149 (25), 1982-2001 (2024)
   PUBMED   38390737
  REMARK    GeneRIF: Targeting NPM1 Epigenetically Promotes Postinfarction
            Cardiac Repair by Reprogramming Reparative Macrophage Metabolism.
REFERENCE   3  (bases 1 to 1320)
  AUTHORS   Kapitonova,A.A., Perfilova,K.V., Cooley,R.B. and Sluchanko,N.N.
  TITLE     Phosphorylation Code of Human Nucleophosmin Includes Four Cryptic
            Sites for Hierarchical Binding of 14-3-3 Proteins
  JOURNAL   J Mol Biol 436 (12), 168592 (2024)
   PUBMED   38702038
  REMARK    GeneRIF: Phosphorylation Code of Human Nucleophosmin Includes Four
            Cryptic Sites for Hierarchical Binding of 14-3-3 Proteins.
REFERENCE   4  (bases 1 to 1320)
  AUTHORS   Selheim,F., Aasebo,E., Reikvam,H., Bruserud,O. and
            Hernandez-Valladares,M.
  TITLE     Monocytic Differentiation of Human Acute Myeloid Leukemia Cells: A
            Proteomic and Phosphoproteomic Comparison of FAB-M4/M5 Patients
            with and without Nucleophosmin 1 Mutations
  JOURNAL   Int J Mol Sci 25 (10), 5080 (2024)
   PUBMED   38791118
  REMARK    GeneRIF: Monocytic Differentiation of Human Acute Myeloid Leukemia
            Cells: A Proteomic and Phosphoproteomic Comparison of FAB-M4/M5
            Patients with and without Nucleophosmin 1 Mutations.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 1320)
  AUTHORS   Petiti,J., Pignochino,Y., Schiavon,A., Giugliano,E., Berrino,E.,
            Giordano,G., Itri,F., Dragani,M., Cilloni,D. and Lo Iacono,M.
  TITLE     Comprehensive Molecular Profiling of NPM1-Mutated Acute Myeloid
            Leukemia Using RNAseq Approach
  JOURNAL   Int J Mol Sci 25 (7), 3631 (2024)
   PUBMED   38612443
  REMARK    GeneRIF: Comprehensive Molecular Profiling of NPM1-Mutated Acute
            Myeloid Leukemia Using RNAseq Approach.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 1320)
  AUTHORS   Fankhauser,C., Izaurralde,E., Adachi,Y., Wingfield,P. and
            Laemmli,U.K.
  TITLE     Specific complex of human immunodeficiency virus type 1 rev and
            nucleolar B23 proteins: dissociation by the Rev response element
  JOURNAL   Mol Cell Biol 11 (5), 2567-2575 (1991)
   PUBMED   2017166
REFERENCE   7  (bases 1 to 1320)
  AUTHORS   Venkatesh,L.K., Mohammed,S. and Chinnadurai,G.
  TITLE     Functional domains of the HIV-1 rev gene required for
            trans-regulation and subcellular localization
  JOURNAL   Virology 176 (1), 39-47 (1990)
   PUBMED   2109912
REFERENCE   8  (bases 1 to 1320)
  AUTHORS   Cochrane,A.W., Perkins,A. and Rosen,C.A.
  TITLE     Identification of sequences important in the nucleolar localization
            of human immunodeficiency virus Rev: relevance of nucleolar
            localization to function
  JOURNAL   J Virol 64 (2), 881-885 (1990)
   PUBMED   2404140
REFERENCE   9  (bases 1 to 1320)
  AUTHORS   Zhang,X.X., Thomis,D.C. and Samuel,C.E.
  TITLE     Isolation and characterization of a molecular cDNA clone of a human
            mRNA from interferon-treated cells encoding nucleolar protein B23,
            numatrin
  JOURNAL   Biochem Biophys Res Commun 164 (1), 176-184 (1989)
   PUBMED   2478125
REFERENCE   10 (bases 1 to 1320)
  AUTHORS   Chan,P.K., Chan,W.Y., Yung,B.Y., Cook,R.G., Aldrich,M.B., Ku,D.,
            Goldknopf,I.L. and Busch,H.
  TITLE     Amino acid sequence of a specific antigenic peptide of protein B23
  JOURNAL   J Biol Chem 261 (30), 14335-14341 (1986)
   PUBMED   2429957
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff in
            collaboration with Francesco Amaldi. The reference sequence was
            derived from AC091980.4 and AC093246.3.
            
            On May 6, 2020 this sequence version replaced NM_002520.6.
            
            Summary: The protein encoded by this gene is involved in several
            cellular processes, including centrosome duplication, protein
            chaperoning, and cell proliferation. The encoded phosphoprotein
            shuttles between the nucleolus, nucleus, and cytoplasm, chaperoning
            ribosomal proteins and core histones from the nucleus to the
            cytoplasm. This protein is also known to sequester the tumor
            suppressor ARF in the nucleolus, protecting it from degradation
            until it is needed. Mutations in this gene are associated with
            acute myeloid leukemia. Dozens of pseudogenes of this gene have
            been identified. [provided by RefSeq, Aug 2017].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1). Variants 1 and 7 both encode the same isoform (1).
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC008495.1, M26697.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000296930.10/ ENSP00000296930.5
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-158               AC091980.4         138242-138399
            159-238             AC091980.4         140444-140523
            239-358             AC091980.4         141698-141817
            359-452             AC091980.4         142099-142192
            453-559             AC091980.4         143103-143209
            560-624             AC091980.4         143307-143371
            625-682             AC091980.4         150546-150603
            683-769             AC091980.4         151232-151318
            770-871             AC093246.3         89669-89770         c
            872-946             AC093246.3         87298-87372         c
            947-1320            AC093246.3         84172-84545         c
FEATURES             Location/Qualifiers
     source          1..1320
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q35.1"
     gene            1..1320
                     /gene="NPM1"
                     /gene_synonym="B23; NPM"
                     /note="nucleophosmin 1"
                     /db_xref="GeneID:4869"
                     /db_xref="HGNC:HGNC:7910"
                     /db_xref="MIM:164040"
     exon            1..158
                     /gene="NPM1"
                     /gene_synonym="B23; NPM"
                     /inference="alignment:Splign:2.1.0"
     CDS             101..985
                     /gene="NPM1"
                     /gene_synonym="B23; NPM"
                     /note="isoform 1 is encoded by transcript variant 1;
                     nucleophosmin/nucleoplasmin family, member 1; nucleolar
                     protein NO38; testicular tissue protein Li 128;
                     nucleophosmin (nucleolar phosphoprotein B23, numatrin)"
                     /codon_start=1
                     /product="nucleophosmin isoform 1"
                     /protein_id="NP_002511.1"
                     /db_xref="CCDS:CCDS4376.1"
                     /db_xref="GeneID:4869"
                     /db_xref="HGNC:HGNC:7910"
                     /db_xref="MIM:164040"
                     /translation="MEDSMDMDMSPLRPQNYLFGCELKADKDYHFKVDNDENEHQLSL
                     RTVSLGAGAKDELHIVEAEAMNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRL
                     KCGSGPVHISGQHLVAVEEDAESEDEEEEDVKLLSISGKRSAPGGGSKVPQKKVKLAA
                     DEDDDDDDEEDDDEDDDDDDFDDEEAEEKAPVKKSIRDTPAKNAQKSNQNGKDSKPSS
                     TPRSKGQESFKKQEKTPKTPKGPSSVEDIKAKMQASIEKGGSLPKVEAKFINYVKNCF
                     RMTDQEAIQDLWQWRKSL"
     exon            159..238
                     /gene="NPM1"
                     /gene_synonym="B23; NPM"
                     /inference="alignment:Splign:2.1.0"
     exon            239..358
                     /gene="NPM1"
                     /gene_synonym="B23; NPM"
                     /inference="alignment:Splign:2.1.0"
     exon            359..452
                     /gene="NPM1"
                     /gene_synonym="B23; NPM"
                     /inference="alignment:Splign:2.1.0"
     exon            453..559
                     /gene="NPM1"
                     /gene_synonym="B23; NPM"
                     /inference="alignment:Splign:2.1.0"
     exon            560..624
                     /gene="NPM1"
                     /gene_synonym="B23; NPM"
                     /inference="alignment:Splign:2.1.0"
     exon            625..682
                     /gene="NPM1"
                     /gene_synonym="B23; NPM"
                     /inference="alignment:Splign:2.1.0"
     exon            683..769
                     /gene="NPM1"
                     /gene_synonym="B23; NPM"
                     /inference="alignment:Splign:2.1.0"
     exon            770..871
                     /gene="NPM1"
                     /gene_synonym="B23; NPM"
                     /inference="alignment:Splign:2.1.0"
     exon            872..946
                     /gene="NPM1"
                     /gene_synonym="B23; NPM"
                     /inference="alignment:Splign:2.1.0"
     exon            947..1320
                     /gene="NPM1"
                     /gene_synonym="B23; NPM"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1296..1301
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NPM1"
                     /gene_synonym="B23; NPM"
                     /note="hexamer: AATAAA"
     polyA_site      1320
                     /gene="NPM1"
                     /gene_synonym="B23; NPM"
                     /note="major polyA site"
ORIGIN      
        1 ctttccctgg tgtgattccg tcctgcgcgg ttgttctctg gagcagcgtt cttttatctc
       61 cgtccgcctt ctctcctacc taagtgcgtg ccgccacccg atggaagatt cgatggacat
      121 ggacatgagc cccctgaggc cccagaacta tcttttcggt tgtgaactaa aggccgacaa
      181 agattatcac tttaaggtgg ataatgatga aaatgagcac cagttatctt taagaacggt
      241 cagtttaggg gctggtgcaa aggatgagtt gcacattgtt gaagcagagg caatgaatta
      301 cgaaggcagt ccaattaaag taacactggc aactttgaaa atgtctgtac agccaacggt
      361 ttcccttggg ggctttgaaa taacaccacc agtggtctta aggttgaagt gtggttcagg
      421 gccagtgcat attagtggac agcacttagt agctgtggag gaagatgcag agtcagaaga
      481 tgaagaggag gaggatgtga aactcttaag tatatctgga aagcggtctg cccctggagg
      541 tggtagcaag gttccacaga aaaaagtaaa acttgctgct gatgaagatg atgacgatga
      601 tgatgaagag gatgatgatg aagatgatga tgatgatgat tttgatgatg aggaagctga
      661 agaaaaagcg ccagtgaaga aatctatacg agatactcca gccaaaaatg cacaaaagtc
      721 aaatcagaat ggaaaagact caaaaccatc atcaacacca agatcaaaag gacaagaatc
      781 cttcaagaaa caggaaaaaa ctcctaaaac accaaaagga cctagttctg tagaagacat
      841 taaagcaaaa atgcaagcaa gtatagaaaa aggtggttct cttcccaaag tggaagccaa
      901 attcatcaat tatgtgaaga attgcttccg gatgactgac caagaggcta ttcaagatct
      961 ctggcagtgg aggaagtctc tttaagaaaa tagtttaaac aatttgttaa aaaattttcc
     1021 gtcttatttc atttctgtaa cagttgatat ctggctgtcc tttttataat gcagagtgag
     1081 aactttccct accgtgtttg ataaatgttg tccaggttct attgccaaga atgtgttgtc
     1141 caaaatgcct gtttagtttt taaagatgga actccaccct ttgcttggtt ttaagtatgt
     1201 atggaatgtt atgataggac atagtagtag cggtggtcag acatggaaat ggtggggaga
     1261 caaaaatata catgtgaaat aaaactcagt attttaataa agtagcacgg tttctattga
//
