LOCUS       NM_004646               5535 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens NPHS1 adhesion molecule, nephrin (NPHS1), mRNA.
ACCESSION   NM_004646
VERSION     NM_004646.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5535)
  AUTHORS   Horinouchi,T., Nagano,C. and Watts,A.J.B.
  TITLE     Anti-nephrin antibodies in steroid-sensitive nephrotic syndrome in
            Japanese children
  JOURNAL   Pediatr Nephrol 39 (1), 337 (2024)
   PUBMED   37495741
  REMARK    GeneRIF: Anti-nephrin antibodies in steroid-sensitive nephrotic
            syndrome in Japanese children.
REFERENCE   2  (bases 1 to 5535)
  AUTHORS   Imeri,F., Stepanovska Tanturovska,B., Manaila,R., Pavenstadt,H.,
            Pfeilschifter,J. and Huwiler,A.
  TITLE     Loss of S1P Lyase Expression in Human Podocytes Causes a Reduction
            in Nephrin Expression That Involves PKCdelta Activation
  JOURNAL   Int J Mol Sci 24 (4), 3267 (2023)
   PUBMED   36834691
  REMARK    GeneRIF: Loss of S1P Lyase Expression in Human Podocytes Causes a
            Reduction in Nephrin Expression That Involves PKCdelta Activation.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5535)
  AUTHORS   Kim,J.Y., Lee,E.J., Seo,J., Lee,Y., Ahn,Y., Park,S., Bae,Y.J.,
            Lee,J., Lim,B.J., Kim,D., Cho,J.W. and Oh,S.H.
  TITLE     Nephrin expression in human epidermal keratinocytes and its
            implication in poor wound closure
  JOURNAL   FASEB J 36 (7), e22424 (2022)
   PUBMED   35747929
  REMARK    GeneRIF: Nephrin expression in human epidermal keratinocytes and
            its implication in poor wound closure.
REFERENCE   4  (bases 1 to 5535)
  AUTHORS   El-Refaey,A.M., Elsamanoudy,A.Z., Elmorsy,Z., Gaber,E., Sarhan,A.,
            Hammad,A., Zedan,M.M. and Bakr,A.
  TITLE     Clinicopathological Impact of Gene Polymorphism of Nephrin and
            Glucocorticoid Receptor Genes in Egyptian Children with Nonfamilial
            Nephrotic Syndrome
  JOURNAL   Saudi J Kidney Dis Transpl 32 (6), 1615-1627 (2021)
   PUBMED   35946274
  REMARK    GeneRIF: Clinicopathological Impact of Gene Polymorphism of Nephrin
            and Glucocorticoid Receptor Genes in Egyptian Children with
            Nonfamilial Nephrotic Syndrome.
REFERENCE   5  (bases 1 to 5535)
  AUTHORS   Aya,K., Tanaka,H. and Seino,Y.
  TITLE     Novel mutation in the nephrin gene of a Japanese patient with
            congenital nephrotic syndrome of the Finnish type
  JOURNAL   Kidney Int 57 (2), 401-404 (2000)
   PUBMED   10652016
REFERENCE   6  (bases 1 to 5535)
  AUTHORS   Bolk,S., Puffenberger,E.G., Hudson,J., Morton,D.H. and
            Chakravarti,A.
  TITLE     Elevated frequency and allelic heterogeneity of congenital
            nephrotic syndrome, Finnish type, in the old order Mennonites
  JOURNAL   Am J Hum Genet 65 (6), 1785-1790 (1999)
   PUBMED   10577936
REFERENCE   7  (bases 1 to 5535)
  AUTHORS   Holthofer,H., Ahola,H., Solin,M.L., Wang,S., Palmen,T., Luimula,P.,
            Miettinen,A. and Kerjaschki,D.
  TITLE     Nephrin localizes at the podocyte filtration slit area and is
            characteristically spliced in the human kidney
  JOURNAL   Am J Pathol 155 (5), 1681-1687 (1999)
   PUBMED   10550324
REFERENCE   8  (bases 1 to 5535)
  AUTHORS   Ruotsalainen,V., Ljungberg,P., Wartiovaara,J., Lenkkeri,U.,
            Kestila,M., Jalanko,H., Holmberg,C. and Tryggvason,K.
  TITLE     Nephrin is specifically located at the slit diaphragm of glomerular
            podocytes
  JOURNAL   Proc Natl Acad Sci U S A 96 (14), 7962-7967 (1999)
   PUBMED   10393930
REFERENCE   9  (bases 1 to 5535)
  AUTHORS   Lenkkeri,U., Mannikko,M., McCready,P., Lamerdin,J., Gribouval,O.,
            Niaudet,P.M., Antignac,C. K, Kashtan,C.E., Homberg,C., Olsen,A.,
            Kestila,M. and Tryggvason,K.
  TITLE     Structure of the gene for congenital nephrotic syndrome of the
            finnish type (NPHS1) and characterization of mutations
  JOURNAL   Am J Hum Genet 64 (1), 51-61 (1999)
   PUBMED   9915943
REFERENCE   10 (bases 1 to 5535)
  AUTHORS   Kestila,M., Lenkkeri,U., Mannikko,M., Lamerdin,J., McCready,P.,
            Putaala,H., Ruotsalainen,V., Morita,T., Nissinen,M., Herva,R.,
            Kashtan,C.E., Peltonen,L., Holmberg,C., Olsen,A. and Tryggvason,K.
  TITLE     Positionally cloned gene for a novel glomerular
            protein--nephrin--is mutated in congenital nephrotic syndrome
  JOURNAL   Mol Cell 1 (4), 575-582 (1998)
   PUBMED   9660941
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC002133.1 and U95090.1.
            
            On Mar 24, 2020 this sequence version replaced NM_004646.3.
            
            Summary: This gene encodes a member of the immunoglobulin family of
            cell adhesion molecules that functions in the glomerular filtration
            barrier in the kidney. The gene is primarily expressed in renal
            tissues, and the protein is a type-1 transmembrane protein found at
            the slit diaphragm of glomerular podocytes. The slit diaphragm is
            thought to function as an ultrafilter to exclude albumin and other
            plasma macromolecules in the formation of urine. Mutations in this
            gene result in Finnish-type congenital nephrosis 1, characterized
            by severe proteinuria and loss of the slit diaphragm and foot
            processes.[provided by RefSeq, Oct 2009].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF035835.1, EU642886.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000378910.10/ ENSP00000368190.4
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 5' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-725               AC002133.1         27252-27976         c
            726-941             AC002133.1         26929-27144         c
            942-1064            AC002133.1         26734-26856         c
            1065-1193           AC002133.1         26433-26561         c
            1194-1275           AC002133.1         25836-25917         c
            1276-1379           AC002133.1         25022-25125         c
            1380-1507           AC002133.1         24708-24835         c
            1508-1679           AC002133.1         24448-24619         c
            1680-1837           AC002133.1         24109-24266         c
            1838-1982           AC002133.1         23725-23869         c
            1983-2107           AC002133.1         23513-23637         c
            2108-2294           AC002133.1         21480-21666         c
            2295-2424           AC002133.1         21141-21270         c
            2425-2597           AC002133.1         20840-21012         c
            2598-2738           AC002133.1         19791-19931         c
            2739-2879           AC002133.1         19575-19715         c
            2880-3001           AC002133.1         18944-19065         c
            3002-3173           AC002133.1         17851-18022         c
            3174-3330           AC002133.1         17596-17752         c
            3331-3482           AC002133.1         17187-17338         c
            3483-3594           AC002133.1         14968-15079         c
            3595-3776           AC002133.1         14709-14890         c
            3777-3833           U95090.1           37305-37361         c
            3834-3953           U95090.1           33243-33362         c
            3954-3978           U95090.1           33076-33100         c
            3979-4054           U95090.1           32896-32971         c
            4055-4148           U95090.1           32653-32746         c
            4149-4261           U95090.1           32444-32556         c
            4262-5535           U95090.1           26972-28245         c
FEATURES             Location/Qualifiers
     source          1..5535
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="19"
                     /map="19q13.12"
     gene            1..5535
                     /gene="NPHS1"
                     /gene_synonym="CNF; nephrin; NPHN"
                     /note="NPHS1 adhesion molecule, nephrin"
                     /db_xref="GeneID:4868"
                     /db_xref="HGNC:HGNC:7908"
                     /db_xref="MIM:602716"
     exon            1..725
                     /gene="NPHS1"
                     /gene_synonym="CNF; nephrin; NPHN"
                     /inference="alignment:Splign:2.1.0"
     CDS             668..4393
                     /gene="NPHS1"
                     /gene_synonym="CNF; nephrin; NPHN"
                     /note="renal glomerulus-specific cell adhesion receptor;
                     nephrosis 1, congenital, Finnish type (nephrin); NPHS1,
                     nephrin"
                     /codon_start=1
                     /product="nephrin precursor"
                     /protein_id="NP_004637.1"
                     /db_xref="CCDS:CCDS32996.1"
                     /db_xref="GeneID:4868"
                     /db_xref="HGNC:HGNC:7908"
                     /db_xref="MIM:602716"
                     /translation="MALGTTLRASLLLLGLLTEGLAQLAIPASVPRGFWALPENLTVV
                     EGASVELRCGVSTPGSAVQWAKDGLLLGPDPRIPGFPRYRLEGDPARGEFHLHIEACD
                     LSDDAEYECQVGRSEMGPELVSPRVILSILVPPKLLLLTPEAGTMVTWVAGQEYVVNC
                     VSGDAKPAPDITILLSGQTISDISANVNEGSQQKLFTVEATARVTPRSSDNRQLLVCE
                     ASSPALEAPIKASFTVNVLFPPGPPVIEWPGLDEGHVRAGQSLELPCVARGGNPLATL
                     QWLKNGQPVSTAWGTEHTQAVARSVLVMTVRPEDHGAQLSCEAHNSVSAGTQEHGITL
                     QVTFPPSAIIILGSASQTENKNVTLSCVSKSSRPRVLLRWWLGWRQLLPMEETVMDGL
                     HGGHISMSNLTFLARREDNGLTLTCEAFSEAFTKETFKKSLILNVKYPAQKLWIEGPP
                     EGQKLRAGTRVRLVCLAIGGNPEPSLMWYKDSRTVTESRLPQESRRVHLGSVEKSGST
                     FSRELVLVTGPSDNQAKFTCKAGQLSASTQLAVQFPPTNVTILANASALRPGDALNLT
                     CVSVSSNPPVNLSWDKEGERLEGVAAPPRRAPFKGSAAARSVLLQVSSRDHGQRVTCR
                     AHSAELRETVSSFYRLNVLYRPEFLGEQVLVVTAVEQGEALLPVSVSANPAPEAFNWT
                     FRGYRLSPAGGPRHRILSSGALHLWNVTRADDGLYQLHCQNSEGTAEARLRLDVHYAP
                     TIRALQDPTEVNVGGSVDIVCTVDANPILPGMFNWERLGEDEEDQSLDDMEKISRGPT
                     GRLRIHHAKLAQAGAYQCIVDNGVAPPARRLLRLVVRFAPQVEHPTPLTKVAAAGDST
                     SSATLHCRARGVPNIVFTWTKNGVPLDLQDPRYTEHTYHQGGVHSSLLTIANVSAAQD
                     YALFTCTATNALGSDQTNIQLVSISRPDPPSGLKVVSLTPHSVGLEWKPGFDGGLPQR
                     FCIRYEALGTPGFHYVDVVPPQATTFTLTGLQPSTRYRVWLLASNALGDSGLADKGTQ
                     LPITTPGLHQPSGEPEDQLPTEPPSGPSGLPLLPVLFALGGLLLLSNASCVGGVLWQR
                     RLRRLAEGISEKTEAGSEEDRVRNEYEESQWTGERDTQSSTVSTTEAEPYYRSLRDFS
                     PQLPPTQEEVSYSRGFTGEDEDMAFPGHLYDEVERTYPPSGAWGPLYDEVQMGPWDLH
                     WPEDTYQDPRGIYDQVAGDLDTLEPDSLPFELRGHLV"
     exon            726..941
                     /gene="NPHS1"
                     /gene_synonym="CNF; nephrin; NPHN"
                     /inference="alignment:Splign:2.1.0"
     exon            942..1064
                     /gene="NPHS1"
                     /gene_synonym="CNF; nephrin; NPHN"
                     /inference="alignment:Splign:2.1.0"
     exon            1065..1193
                     /gene="NPHS1"
                     /gene_synonym="CNF; nephrin; NPHN"
                     /inference="alignment:Splign:2.1.0"
     exon            1194..1275
                     /gene="NPHS1"
                     /gene_synonym="CNF; nephrin; NPHN"
                     /inference="alignment:Splign:2.1.0"
     exon            1276..1379
                     /gene="NPHS1"
                     /gene_synonym="CNF; nephrin; NPHN"
                     /inference="alignment:Splign:2.1.0"
     exon            1380..1507
                     /gene="NPHS1"
                     /gene_synonym="CNF; nephrin; NPHN"
                     /inference="alignment:Splign:2.1.0"
     exon            1508..1679
                     /gene="NPHS1"
                     /gene_synonym="CNF; nephrin; NPHN"
                     /inference="alignment:Splign:2.1.0"
     exon            1680..1837
                     /gene="NPHS1"
                     /gene_synonym="CNF; nephrin; NPHN"
                     /inference="alignment:Splign:2.1.0"
     exon            1838..1982
                     /gene="NPHS1"
                     /gene_synonym="CNF; nephrin; NPHN"
                     /inference="alignment:Splign:2.1.0"
     exon            1983..2107
                     /gene="NPHS1"
                     /gene_synonym="CNF; nephrin; NPHN"
                     /inference="alignment:Splign:2.1.0"
     exon            2108..2294
                     /gene="NPHS1"
                     /gene_synonym="CNF; nephrin; NPHN"
                     /inference="alignment:Splign:2.1.0"
     exon            2295..2424
                     /gene="NPHS1"
                     /gene_synonym="CNF; nephrin; NPHN"
                     /inference="alignment:Splign:2.1.0"
     exon            2425..2597
                     /gene="NPHS1"
                     /gene_synonym="CNF; nephrin; NPHN"
                     /inference="alignment:Splign:2.1.0"
     exon            2598..2738
                     /gene="NPHS1"
                     /gene_synonym="CNF; nephrin; NPHN"
                     /inference="alignment:Splign:2.1.0"
     exon            2739..2879
                     /gene="NPHS1"
                     /gene_synonym="CNF; nephrin; NPHN"
                     /inference="alignment:Splign:2.1.0"
     exon            2880..3001
                     /gene="NPHS1"
                     /gene_synonym="CNF; nephrin; NPHN"
                     /inference="alignment:Splign:2.1.0"
     exon            3002..3173
                     /gene="NPHS1"
                     /gene_synonym="CNF; nephrin; NPHN"
                     /inference="alignment:Splign:2.1.0"
     exon            3174..3330
                     /gene="NPHS1"
                     /gene_synonym="CNF; nephrin; NPHN"
                     /inference="alignment:Splign:2.1.0"
     exon            3331..3482
                     /gene="NPHS1"
                     /gene_synonym="CNF; nephrin; NPHN"
                     /inference="alignment:Splign:2.1.0"
     exon            3483..3594
                     /gene="NPHS1"
                     /gene_synonym="CNF; nephrin; NPHN"
                     /inference="alignment:Splign:2.1.0"
     exon            3595..3776
                     /gene="NPHS1"
                     /gene_synonym="CNF; nephrin; NPHN"
                     /inference="alignment:Splign:2.1.0"
     exon            3777..3833
                     /gene="NPHS1"
                     /gene_synonym="CNF; nephrin; NPHN"
                     /inference="alignment:Splign:2.1.0"
     exon            3834..3953
                     /gene="NPHS1"
                     /gene_synonym="CNF; nephrin; NPHN"
                     /inference="alignment:Splign:2.1.0"
     exon            3954..3978
                     /gene="NPHS1"
                     /gene_synonym="CNF; nephrin; NPHN"
                     /inference="alignment:Splign:2.1.0"
     exon            3979..4054
                     /gene="NPHS1"
                     /gene_synonym="CNF; nephrin; NPHN"
                     /inference="alignment:Splign:2.1.0"
     exon            4055..4148
                     /gene="NPHS1"
                     /gene_synonym="CNF; nephrin; NPHN"
                     /inference="alignment:Splign:2.1.0"
     exon            4149..4261
                     /gene="NPHS1"
                     /gene_synonym="CNF; nephrin; NPHN"
                     /inference="alignment:Splign:2.1.0"
     exon            4262..5535
                     /gene="NPHS1"
                     /gene_synonym="CNF; nephrin; NPHN"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5214..5219
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NPHS1"
                     /gene_synonym="CNF; nephrin; NPHN"
                     /note="hexamer: AATAAA"
     polyA_site      5247
                     /gene="NPHS1"
                     /gene_synonym="CNF; nephrin; NPHN"
                     /note="major polyA site"
     regulatory      5512..5517
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NPHS1"
                     /gene_synonym="CNF; nephrin; NPHN"
                     /note="hexamer: AATAAA"
     polyA_site      5535
                     /gene="NPHS1"
                     /gene_synonym="CNF; nephrin; NPHN"
ORIGIN      
        1 gagaatgagc tcaagctggg tcagagagca gggctgactc tgccagtgcc tgcatcagcc
       61 tcatcgctct cctaggctcc tggcctgctg gactctgggc tgcaggtcct tcttgaaagg
      121 ctgtgagtag tgagacaagg agcaggagtg aggggtggca ggagagaaga tagagattga
      181 gagagagaga gagagagaga cagagagaga ggaagagaca gagacaaaag gagagagaac
      241 ggcttagaca aggagagaaa gatggaaaga taaagagact gggcgcagtg gctcacgcct
      301 gtaatcccaa cacttgggga ggccaaggtg ggaggatggc ttgaaggaaa gagtctgaga
      361 tcaacctggc caacatagtg agaccccgtc tctaaaaaaa aaaaaaagaa aaaaaaaaga
      421 aaaaagaaaa aaaagttttt ttaaagagac agagaaagag actcagagat tgagactgag
      481 agcaagacag agagagatac tcacagggaa gaggggaaga ggaaaacgag aaagggagga
      541 gagtaacgga aagagataaa aaagaaaagc aggtggcaga gacacacaga gagggaccca
      601 gagaaagcca gacagacgca ggtggctggc agcgggcgct gtgggggtca cagtaggggg
      661 acctgtgatg gccctgggga cgacgctcag ggcttctctc ctgctcctgg ggctgctgac
      721 tgaaggcctg gcgcagttgg cgattcctgc ctccgttccc cggggcttct gggccctgcc
      781 tgaaaacctg acggtggtgg agggggcctc agtggagctg cgttgtgggg tcagcacccc
      841 tggcagtgcg gtgcaatggg ccaaagatgg gctgctcctg ggccccgacc ccaggatccc
      901 aggcttcccg aggtaccgcc tggaagggga ccctgctaga ggtgaattcc acctgcacat
      961 cgaggcctgt gacctcagcg atgacgcgga gtatgagtgc caggtcggcc gctctgagat
     1021 ggggcccgag ctcgtgtctc ccagagtgat cctctccatc ctggttcctc ccaagctgct
     1081 cctgctgacc ccagaggcag gcaccatggt cacctgggta gctgggcagg agtacgtggt
     1141 caactgtgtg tctggggacg cgaagccagc acctgacatc accattctcc tgagtggaca
     1201 gacaatatct gacatctctg caaacgtgaa cgagggctcc cagcagaaac tcttcactgt
     1261 ggaggccaca gccagggtga caccccggag ctcagataat aggcagttgc tggtctgtga
     1321 ggcgtctagc ccagcactgg aggcccccat caaggcctca ttcaccgtga atgttctgtt
     1381 ccctccagga ccccctgtca tcgagtggcc aggcctggat gaggggcacg tgcgggcagg
     1441 acagagcttg gagctgccgt gcgtggcccg agggggtaat cccttagcca cactgcagtg
     1501 gctgaagaat ggccagccgg tgtccacagc gtggggcaca gagcacaccc aggcggtggc
     1561 ccgcagtgtg ctggtgatga ccgtgaggcc agaagaccat ggagcgcagc tcagctgcga
     1621 ggcccacaac agcgtgtctg cagggaccca ggagcacggc atcacactgc aggtcacctt
     1681 tccccctagt gccattatta tcttgggatc tgcatcccag actgagaaca agaacgtgac
     1741 actctcctgt gtcagcaagt ccagtcgccc gcgggttctg ctacgatggt ggctgggctg
     1801 gcggcagctg ctgcccatgg aggagacagt catggatgga ctgcatggcg gtcacatctc
     1861 catgtccaac ctgacattcc tggcgcggcg ggaggacaac ggtctgaccc tcacatgtga
     1921 ggccttcagt gaagccttca ccaaggagac cttcaagaag tcgctcatcc tgaacgtaaa
     1981 atatcccgcc cagaaactgt ggattgaggg tcccccagag ggccagaagc tccgggctgg
     2041 gacccgggtg aggctggtgt gtttggctat cgggggcaac ccagagccct ccctcatgtg
     2101 gtacaaggac tcgcgcaccg tgaccgagtc gcggctgccg caggagtcgc ggcgcgtgca
     2161 tctcggcagc gtggagaaat ctgggagcac cttctcccga gagctggtgc tggtcacagg
     2221 gccgtcggac aaccaggcca agttcacgtg caaggctgga cagctcagcg cgtccacgca
     2281 gctggcggtg cagtttcccc caactaacgt gacgatcctg gccaacgcat ccgcactgcg
     2341 cccgggagac gccttaaact tgacatgcgt cagcgtcagc agcaatccgc cggtcaactt
     2401 gtcctgggac aaggaagggg agaggctgga gggcgtggcc gccccacccc ggagagcccc
     2461 attcaaaggc tccgccgccg ccaggagcgt ccttctgcaa gtgtcatccc gcgatcatgg
     2521 ccagcgcgtg acctgccgcg cccacagcgc cgagctccgc gaaaccgtga gctccttcta
     2581 tcgcctcaac gtactgtacc gtccagagtt cctgggggag caggtgctgg tggtgaccgc
     2641 ggtggagcag ggcgaggcgt tgctgcccgt gtccgtgtcc gctaaccccg cccccgaggc
     2701 cttcaactgg accttccgcg gctatcgcct cagtccagcg ggcggccccc ggcatcgcat
     2761 cctgtccagc ggggctctgc atctgtggaa tgtgacccgc gcggacgacg gcctctatca
     2821 gctgcactgc cagaactctg agggcaccgc ggaagcgcgg ctgcggctgg acgtgcacta
     2881 tgctcccacc atccgtgccc tccaggaccc cactgaggtg aacgtcgggg gttctgtgga
     2941 catagtctgc actgtcgatg ccaatcccat cctcccgggc atgttcaact gggagagact
     3001 gggagaagat gaggaggacc agagcctgga tgacatggag aagatatcca ggggaccaac
     3061 ggggcgcctg cggattcacc atgccaaact ggcccaggct ggcgcttacc agtgcattgt
     3121 ggacaatggg gtggcgcctc cagcacgacg gctgctccgt cttgttgtca gatttgcccc
     3181 ccaggtggag caccccactc ccctaactaa ggtggctgca gctggagaca gcaccagttc
     3241 tgccaccctc cactgccgtg cccgaggtgt ccccaacatc gttttcactt ggacaaaaaa
     3301 cggggtccct ctggatctcc aagatcccag gtacacggag cacacatacc accagggtgg
     3361 tgtccacagc agcctcctga ccattgccaa cgtgtctgcc gcccaggatt acgccctctt
     3421 cacatgtaca gccaccaacg cccttggctc ggaccaaacc aacattcaac ttgtcagcat
     3481 cagccgccct gaccctccat caggattaaa ggttgtgagt ctgaccccac actccgtggg
     3541 gctggagtgg aagcctggct ttgatggggg cctgccacag aggttctgca tcaggtatga
     3601 ggccctgggg actccagggt tccactatgt ggatgtcgta ccaccccagg ccaccacctt
     3661 cacgctgact ggtctacagc cttctacaag atacagggtc tggctgctgg ccagtaatgc
     3721 cttgggggac agtggactgg ctgacaaagg gacccagctt cccatcacta ccccaggtct
     3781 ccaccagcct tctggagaac ctgaagacca gctgcccaca gagccacctt caggaccctc
     3841 ggggctgccc ctgctgcctg tgctgttcgc tcttgggggg cttctgctcc tctccaatgc
     3901 ctcctgtgtc gggggggtcc tctggcagcg gagactcagg cgtcttgctg agggcatctc
     3961 agagaagaca gaggcagggt cggaagagga ccgagtcagg aacgaatatg aggagagcca
     4021 gtggacagga gagcgggaca ctcagagctc cacggtcagc acaacagagg cagagccgta
     4081 ttaccgctcc ctgagggact tcagccccca gctgcccccg acgcaggagg aggtgtctta
     4141 ttcccgaggt ttcacaggtg aagatgagga tatggccttc cctgggcact tgtatgatga
     4201 ggtagaaaga acgtaccccc cgtctggagc ctggggaccc ctctacgatg aagtgcagat
     4261 gggaccctgg gacctccact ggcctgaaga cacatatcag gatccaagag gaatctatga
     4321 ccaggtggcc ggagacttgg acactctgga acccgattct ctgcccttcg agctgagggg
     4381 acatctggtg taagagccct ctcaacccca ttgtcctgca cctgcaggaa tttacactcc
     4441 actggtctct ctcattacag cctgggccga gctggttagg tgagctccat aaaacccaaa
     4501 gggacttggt gtcaggagag gacatggagg gggctgagtg acagagatgg ttcagctggt
     4561 accagagtag aaacaaggtg catcctgggg ttggctttag aaactaaact tctccaaaag
     4621 gacagggcag attgtaaacg tcgtctcaaa aatgaaatgc tgccgggtgc ggtgactcac
     4681 gcctataatc ccagcacttt gggaggctga ggcgggtgga tcacctgagg tcaggagttc
     4741 gagaccagcc tggccaacat ggtaaaactc catttctact aaaaatataa aaaattagcc
     4801 aggagtagtg gcgcatgcct gtagtcccag ctacttggga ggctgatgca tgagaattgc
     4861 ttgaacccag gaggcggagg ttgcagtgag ctgagatcac gccactgcac tccagcctgg
     4921 gcgacagagc gagattctgt ctcaaaaaat aaaaataaaa taaaaataaa aaataaaatg
     4981 ctattctgag ggtccctaag tgcttcatga agtaaagttt tcagaagtgg ggaaagacat
     5041 atgaaaaact ctgttcattt tgcattccat gacgtctgaa atagctaata ataagtggaa
     5101 tttatgaatc catgttgcat cctacctatt attttattat ttccgacagt tatctaacac
     5161 aggaggcata aactatgaca catgtaaaca taagtatgct ccataagcat taaaataaac
     5221 agacaatcaa aagaagctag aggaaaagaa tatgtactgt gtgtgtgctt tcgtttatag
     5281 aaattctaaa ataggcaaaa ctaatctacg gtggcagaga gcacagcaga ggtttcctgg
     5341 ggccatgaga ggagggttga ctgcaaaaag gcatgggaga actttctgag gtgttggaaa
     5401 tattctatat aacgattgtg gtggtggttg cacgggtgca cagatttgtc aaaactcatt
     5461 gaactttaca cttaagatgg gtgcattttt attgtacata aatgatatct caataaaagt
     5521 gattttaaaa acagg
//
