LOCUS       NM_015102               4955 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens nephrocystin 4 (NPHP4), transcript variant 1, mRNA.
ACCESSION   NM_015102
VERSION     NM_015102.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4955)
  AUTHORS   Ali,A., Unar,A., Muhammad,Z., Dil,S., Zhang,B., Sadaf,H., Khan,M.,
            Ali,M., Khan,R., Shah,K.M.B., Ma,A., Jiang,X., Zhang,Y., Zhang,H.
            and Shi,Q.
  TITLE     A novel NPHP4 homozygous missense variant identified in infertile
            brothers with multiple morphological abnormalities of the sperm
            flagella
  JOURNAL   J Assist Reprod Genet 41 (1), 109-120 (2024)
   PUBMED   37831349
  REMARK    GeneRIF: A novel NPHP4 homozygous missense variant identified in
            infertile brothers with multiple morphological abnormalities of the
            sperm flagella.
REFERENCE   2  (bases 1 to 4955)
  AUTHORS   Otaki,Y., Watanabe,T., Sato,J., Kobayashi,Y., Aono,T., Saito,Y.,
            Goto,J., Takahashi,H., Arimoto,T., Sato,H., Konta,T., Ueno,Y. and
            Watanabe,M.
  TITLE     Association of Nephronophthisis 4 genetic variation with
            cardiorenal syndrome and cardiovascular events in Japanese general
            population: the Yamagata (Takahata) study
  JOURNAL   Heart Vessels 37 (4), 673-682 (2022)
   PUBMED   34591160
  REMARK    GeneRIF: Association of Nephronophthisis 4 genetic variation with
            cardiorenal syndrome and cardiovascular events in Japanese general
            population: the Yamagata (Takahata) study.
REFERENCE   3  (bases 1 to 4955)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 4955)
  AUTHORS   Hoefele,J., Otto,E., Felten,H., Kuhn,K., Bley,T.A., Zauner,I.,
            Hildebrandt,F. and Neumann,H.P.
  TITLE     Clinical and histological presentation of 3 siblings with mutations
            in the NPHP4 gene
  JOURNAL   Am J Kidney Dis 43 (2), 358-364 (2004)
   PUBMED   14750102
REFERENCE   5  (bases 1 to 4955)
  AUTHORS   Otto,E., Hoefele,J., Ruf,R., Mueller,A.M., Hiller,K.S., Wolf,M.T.,
            Schuermann,M.J., Becker,A., Birkenhager,R., Sudbrak,R.,
            Hennies,H.C., Nurnberg,P. and Hildebrandt,F.
  TITLE     A gene mutated in nephronophthisis and retinitis pigmentosa encodes
            a novel protein, nephroretinin, conserved in evolution
  JOURNAL   Am J Hum Genet 71 (5), 1161-1167 (2002)
   PUBMED   12205563
  REMARK    GeneRIF: Encodes a novel protein, nephroretinin, that is conserved
            in evolution--for example, in the nematode Caenorhabditis elegans.
            GeneRIF: Recessive mutations in the NPHP4 gene, encoding the
            protein nephroretinin, in humans cause nephronophthisis type 4 and
            Senior-Loken syndrome.There is evolutionary conservation of the
            NPHP4 gene, with an ortholog in C. elegans.
REFERENCE   6  (bases 1 to 4955)
  AUTHORS   Mollet,G., Salomon,R., Gribouval,O., Silbermann,F., Bacq,D.,
            Landthaler,G., Milford,D., Nayir,A., Rizzoni,G., Antignac,C. and
            Saunier,S.
  TITLE     The gene mutated in juvenile nephronophthisis type 4 encodes a
            novel protein that interacts with nephrocystin
  JOURNAL   Nat Genet 32 (2), 300-305 (2002)
   PUBMED   12244321
  REMARK    GeneRIF: Interacts with NPHP1 protein, suggesting that these two
            proteins participate in a common signaling pathway; identification
            of five different mutations in unrelated individuals with
            nephronophthisis
            Erratum:[Nat Genet 2002 Nov;32(3):459]
REFERENCE   7  (bases 1 to 4955)
  AUTHORS   Donaldson,J.C., Dise,R.S., Ritchie,M.D. and Hanks,S.K.
  TITLE     Nephrocystin-conserved domains involved in targeting to epithelial
            cell-cell junctions, interaction with filamins, and establishing
            cell polarity
  JOURNAL   J Biol Chem 277 (32), 29028-29035 (2002)
   PUBMED   12006559
REFERENCE   8  (bases 1 to 4955)
  AUTHORS   Schuermann,M.J., Otto,E., Becker,A., Saar,K., Ruschendorf,F.,
            Polak,B.C., Ala-Mello,S., Hoefele,J., Wiedensohler,A., Haller,M.,
            Omran,H., Nurnberg,P. and Hildebrandt,F.
  TITLE     Mapping of gene loci for nephronophthisis type 4 and Senior-Loken
            syndrome, to chromosome 1p36
  JOURNAL   Am J Hum Genet 70 (5), 1240-1246 (2002)
   PUBMED   11920287
REFERENCE   9  (bases 1 to 4955)
  AUTHORS   Stokman,M., Lilien,M. and Knoers,N.
  TITLE     Nephronophthisis-Related Ciliopathies
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   27336129
REFERENCE   10 (bases 1 to 4955)
  AUTHORS   Gunay-Aygun,M., Gahl,W.A. and Heller,T.
  TITLE     Congenital Hepatic Fibrosis Overview horizontal line RETIRED
            CHAPTER, FOR HISTORICAL REFERENCE ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301743
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AF537130.1, AK314326.1,
            BU738621.1 and R40620.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_015102.4.
            
            Summary: This gene encodes a protein involved in renal tubular
            development and function. This protein interacts with nephrocystin,
            and belongs to a multifunctional complex that is localized to
            actin- and microtubule-based structures. Mutations in this gene are
            associated with nephronophthisis type 4, a renal disease, and with
            Senior-Loken syndrome type 4, a combination of nephronophthisis and
            retinitis pigmentosa. Alternative splicing results in multiple
            transcript variants. [provided by RefSeq, Apr 2014].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF537130.1, AY118228.1 [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000378156.9/ ENSP00000367398.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-3021              AF537130.1         47-3067
            3022-3037           AK314326.1         2734-2749
            3038-4948           AF537130.1         3084-4994
            4949-4950           BU738621.1         25-26               c
            4951-4955           R40620.1           18-22               c
FEATURES             Location/Qualifiers
     source          1..4955
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.31"
     gene            1..4955
                     /gene="NPHP4"
                     /gene_synonym="POC10; SLSN4"
                     /note="nephrocystin 4"
                     /db_xref="GeneID:261734"
                     /db_xref="HGNC:HGNC:19104"
                     /db_xref="MIM:607215"
     exon            1..182
                     /gene="NPHP4"
                     /gene_synonym="POC10; SLSN4"
                     /inference="alignment:Splign:2.1.0"
     exon            183..355
                     /gene="NPHP4"
                     /gene_synonym="POC10; SLSN4"
                     /inference="alignment:Splign:2.1.0"
     CDS             221..4501
                     /gene="NPHP4"
                     /gene_synonym="POC10; SLSN4"
                     /note="isoform a is encoded by transcript variant 1; POC10
                     centriolar protein homolog; nephroretinin"
                     /codon_start=1
                     /product="nephrocystin-4 isoform a"
                     /protein_id="NP_055917.1"
                     /db_xref="CCDS:CCDS44052.1"
                     /db_xref="GeneID:261734"
                     /db_xref="HGNC:HGNC:19104"
                     /db_xref="MIM:607215"
                     /translation="MNDWHRIFTQNVLVPPHPQRARQPWKESTAFQCVLKWLDGPVIR
                     QGVLEVLSEVECHLRVSFFDVTYRHFFGRTWKTTVKPTKRPPSRIVFNEPLYFHTSLN
                     HPHIVAVVEVVAEGKKRDGSLQTLSCGFGILRIFSNQPDSPISASQDKRLRLYHGTPR
                     ALLHPLLQDPAEQNRHMTLIENCSLQYTLKPHPALEPAFHLLPENLLVSGLQQIPGLL
                     PAHGESGDALRKPRLQKPITGHLDDLFFTLYPSLEKFEEELLELHVQDHFQEGCGPLD
                     GGALEILERRLRVGVHNGLGFVQRPQVVVLVPEMDVALTRSASFSRKVVSSSKTSSGS
                     QALVLRSRLRLPEMVGHPAFAVIFQLEYVFSSPAGVDGNAASVTSLSNLACMHMVRWA
                     VWNPLLEADSGRVTLPLQGGIQPNPSHCLVYKVPSASMSSEEVKQVESGTLRFQFSLG
                     SEEHLDAPTEPVSGPKVERRPSRKPPTSPSSPPAPVPRVLAAPQNSPVGPGLSISQLA
                     ASPRSPTQHCLARPTSQLPHGSQASPAQAQEFPLEAGISHLEADLSQTSLVLETSIAE
                     QLQELPFTPLHAPIVVGTQTRSSAGQPSRASMVLLQSSGFPEILDANKQPAEAVSATE
                     PVTFNPQKEESDCLQSNEMVLQFLAFSRVAQDCRGTSWPKTVYFTFQFYRFPPATTPR
                     LQLVQLDEAGQPSSGALTHILVPVSRDGTFDAGSPGFQLRYMVGPGFLKPGERRCFAR
                     YLAVQTLQIDVWDGDSLLLIGSAAVQMKHLLRQGRPAVQASHELEVVATEYEQDNMVV
                     SGDMLGFGRVKPIGVHSVVKGRLHLTLANVGHPCEQKVRGCSTLPPSRSRVISNDGAS
                     RFSGGSLLTTGSSRRKHVVQAQKLADVDSELAAMLLTHARQGKGPQDVSRESDATRRR
                     KLERMRSVRLQEAGGDLGRRGTSVLAQQSVRTQHLRDLQVIAAYRERTKAESIASLLS
                     LAITTEHTLHATLGVAEFFEFVLKNPHNTQHTVTVEIDNPELSVIVDSQEWRDFKGAA
                     GLHTPVEEDMFHLRGSLAPQLYLRPHETAHVPFKFQSFSAGQLAMVQASPGLSNEKGM
                     DAVSPWKSSAVPTKHAKVLFRASGGKPIAVLCLTVELQPHVVDQVFRFYHPELSFLKK
                     AIRLPPWHTFPGAPVGMLGEDPPVHVRCSDPNVICETQNVGPGEPRDIFLKVASGPSP
                     EIKDFFVIIYSDRWLATPTQTWQVYLHSLQRVDVSCVAGQLTRLSLVLRGTQTVRKVR
                     AFTSHPQELKTDPKGVFVLPPRGVQDLHVGVRPLRAGSRFVHLNLVDVDCHQLVASWL
                     VCLCCRQPLISKAFEIMLAAGEGKGVNKRITYTNPYPSRRTFHLHSDHPELLRFREDS
                     FQVGGGETYTIGLQFAPSQRVGEEEILIYINDHEDKNEEAFCVKVIYQ"
     exon            356..499
                     /gene="NPHP4"
                     /gene_synonym="POC10; SLSN4"
                     /inference="alignment:Splign:2.1.0"
     exon            500..672
                     /gene="NPHP4"
                     /gene_synonym="POC10; SLSN4"
                     /inference="alignment:Splign:2.1.0"
     exon            673..737
                     /gene="NPHP4"
                     /gene_synonym="POC10; SLSN4"
                     /inference="alignment:Splign:2.1.0"
     exon            738..893
                     /gene="NPHP4"
                     /gene_synonym="POC10; SLSN4"
                     /inference="alignment:Splign:2.1.0"
     exon            894..1030
                     /gene="NPHP4"
                     /gene_synonym="POC10; SLSN4"
                     /inference="alignment:Splign:2.1.0"
     exon            1031..1212
                     /gene="NPHP4"
                     /gene_synonym="POC10; SLSN4"
                     /inference="alignment:Splign:2.1.0"
     exon            1213..1339
                     /gene="NPHP4"
                     /gene_synonym="POC10; SLSN4"
                     /inference="alignment:Splign:2.1.0"
     exon            1340..1522
                     /gene="NPHP4"
                     /gene_synonym="POC10; SLSN4"
                     /inference="alignment:Splign:2.1.0"
     exon            1523..1661
                     /gene="NPHP4"
                     /gene_synonym="POC10; SLSN4"
                     /inference="alignment:Splign:2.1.0"
     exon            1662..1723
                     /gene="NPHP4"
                     /gene_synonym="POC10; SLSN4"
                     /inference="alignment:Splign:2.1.0"
     exon            1724..1831
                     /gene="NPHP4"
                     /gene_synonym="POC10; SLSN4"
                     /inference="alignment:Splign:2.1.0"
     exon            1832..1983
                     /gene="NPHP4"
                     /gene_synonym="POC10; SLSN4"
                     /inference="alignment:Splign:2.1.0"
     exon            1984..2175
                     /gene="NPHP4"
                     /gene_synonym="POC10; SLSN4"
                     /inference="alignment:Splign:2.1.0"
     exon            2176..2363
                     /gene="NPHP4"
                     /gene_synonym="POC10; SLSN4"
                     /inference="alignment:Splign:2.1.0"
     exon            2364..2524
                     /gene="NPHP4"
                     /gene_synonym="POC10; SLSN4"
                     /inference="alignment:Splign:2.1.0"
     exon            2525..2705
                     /gene="NPHP4"
                     /gene_synonym="POC10; SLSN4"
                     /inference="alignment:Splign:2.1.0"
     exon            2706..2831
                     /gene="NPHP4"
                     /gene_synonym="POC10; SLSN4"
                     /inference="alignment:Splign:2.1.0"
     exon            2832..3037
                     /gene="NPHP4"
                     /gene_synonym="POC10; SLSN4"
                     /inference="alignment:Splign:2.1.0"
     exon            3038..3264
                     /gene="NPHP4"
                     /gene_synonym="POC10; SLSN4"
                     /inference="alignment:Splign:2.1.0"
     exon            3265..3451
                     /gene="NPHP4"
                     /gene_synonym="POC10; SLSN4"
                     /inference="alignment:Splign:2.1.0"
     exon            3452..3535
                     /gene="NPHP4"
                     /gene_synonym="POC10; SLSN4"
                     /inference="alignment:Splign:2.1.0"
     exon            3536..3692
                     /gene="NPHP4"
                     /gene_synonym="POC10; SLSN4"
                     /inference="alignment:Splign:2.1.0"
     exon            3693..3778
                     /gene="NPHP4"
                     /gene_synonym="POC10; SLSN4"
                     /inference="alignment:Splign:2.1.0"
     exon            3779..3864
                     /gene="NPHP4"
                     /gene_synonym="POC10; SLSN4"
                     /inference="alignment:Splign:2.1.0"
     exon            3865..4036
                     /gene="NPHP4"
                     /gene_synonym="POC10; SLSN4"
                     /inference="alignment:Splign:2.1.0"
     exon            4037..4216
                     /gene="NPHP4"
                     /gene_synonym="POC10; SLSN4"
                     /inference="alignment:Splign:2.1.0"
     exon            4217..4360
                     /gene="NPHP4"
                     /gene_synonym="POC10; SLSN4"
                     /inference="alignment:Splign:2.1.0"
     exon            4361..4955
                     /gene="NPHP4"
                     /gene_synonym="POC10; SLSN4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4930..4935
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NPHP4"
                     /gene_synonym="POC10; SLSN4"
                     /note="hexamer: AACAAA"
     polyA_site      4955
                     /gene="NPHP4"
                     /gene_synonym="POC10; SLSN4"
                     /note="major polyA site"
ORIGIN      
        1 cttccgaggc tcctcccgcg ggtggcagcg gacggggcgc gcccctcggc cagtcctcgg
       61 tcctcaggct tgtggctccg ttgagcaccg gccgccgggc ctctgggtcc gtcgagtgga
      121 gactctctga aaagcgtggg ctccgtggcc tccggcgcgg ccgcggcggg tcggtctcct
      181 agatcatccg ggaagcccac gggaccctca ggcgggcagg atgaacgact ggcacaggat
      241 cttcacccaa aacgtgcttg tccctcccca cccacagaga gcgcgccagc cttggaagga
      301 atccacggca ttccagtgtg tcctcaagtg gctggacgga ccggtaatta ggcagggcgt
      361 gctggaggta ctgtcagagg ttgaatgcca tctgcgagtg tctttctttg atgtcaccta
      421 ccggcacttc tttgggagga cgtggaaaac cacagtgaag ccgacgaaga gaccgccgtc
      481 caggatcgtc tttaatgagc ccttgtattt tcacacatcc ctaaaccacc ctcatatcgt
      541 ggctgtggtg gaagtggtcg ctgagggcaa gaaacgggat gggagcctcc agacattgtc
      601 ctgtgggttt ggaattcttc ggatcttcag caaccagccg gactctccta tctctgcttc
      661 ccaggacaaa aggttgcggc tgtaccatgg cacccccaga gccctcctgc acccgcttct
      721 ccaggacccc gcagagcaaa acagacacat gaccctcatt gagaactgca gcctgcagta
      781 cacgctgaag ccacacccgg ccctggagcc tgcgttccac cttcttcctg agaaccttct
      841 ggtgtctggt ctgcagcaga tacctggcct gcttccagct catggagaat ccggcgacgc
      901 tctccgaaag cctcgcctcc agaagcccat cacggggcac ttggatgact tattcttcac
      961 cctgtacccc tccctggaga agtttgagga agagctgctg gagctccacg tccaggacca
     1021 cttccaggag ggatgtggcc cactggacgg tggtgccctg gagatcctgg agcggcgcct
     1081 gcgtgtgggc gtgcacaatg gtctgggctt cgtgcagagg ccgcaggtcg ttgtactggt
     1141 gcctgagatg gatgtggcct tgacgcgctc agctagcttc agcaggaaag tggtctcctc
     1201 ttccaagacc agctccggga gccaagctct ggttttgaga agccgcctcc gcctcccaga
     1261 gatggtcggc caccctgcat ttgcggtcat cttccagctg gagtacgtgt tcagcagccc
     1321 tgcaggagtg gacggcaatg cagcttcggt cacctctctg tccaacctgg catgcatgca
     1381 catggtccgc tgggctgttt ggaacccctt gctggaagct gattctggaa gggtgaccct
     1441 gcctctgcag ggtgggatcc agcccaaccc ctcgcactgt ctggtctaca aggtaccctc
     1501 agccagcatg agctctgaag aggtgaagca ggtggagtcg ggtacactcc ggttccagtt
     1561 ctcgctgggc tcagaagaac acctggatgc acccacggag cctgtcagtg gccccaaagt
     1621 ggagcggcgg ccttccagga aaccacccac gtccccttcg agcccgccag cgccagtacc
     1681 tcgagttctc gctgccccgc agaactcacc tgtgggacca gggttgtcaa tttcccagct
     1741 ggcggcctcc ccgcggtccc cgactcagca ctgcttggcc aggcctactt cacagctacc
     1801 ccatggctct caggcctccc cggcccaggc acaggagttc ccgttggagg ccggtatctc
     1861 ccacctggaa gccgacctga gccagacctc cctggtcctg gaaacatcca ttgccgaaca
     1921 gttacaggag ctgccgttca cgcctttgca tgcccctatt gttgtgggaa cccagaccag
     1981 gagctctgca gggcagccct cgagagcctc catggtgctc ctgcagtcct ccggctttcc
     2041 cgagattctg gatgccaata aacagccagc cgaggctgtc agcgctacag aacctgtgac
     2101 gtttaaccct cagaaggaag aatcagattg tctacaaagc aacgagatgg tgctacagtt
     2161 tcttgccttt agcagagtgg cccaggactg ccgaggaaca tcatggccaa agactgtgta
     2221 tttcaccttc cagttctacc gcttcccacc cgcaacgacg ccacgactgc agctggtcca
     2281 gctggatgag gccggccagc ccagctctgg cgccctgacc cacatcctcg tgcctgtgag
     2341 cagagatggc acctttgatg ctgggtctcc tggcttccag ctgaggtaca tggtgggccc
     2401 tgggttcctg aagccaggtg agcggcgctg ctttgcccgc tacctggccg tgcagaccct
     2461 gcagattgac gtctgggacg gagactccct gctgctcatc ggatctgctg ccgtccagat
     2521 gaagcatctc ctccgccaag gccggccggc tgtgcaggcc tcccacgagc ttgaggtcgt
     2581 ggcaactgaa tacgagcagg acaacatggt ggtgagtgga gacatgctgg ggtttggccg
     2641 cgtcaagccc atcggcgtcc actcggtggt gaagggccgg ctgcacctga ctttggccaa
     2701 cgtgggtcac ccgtgtgaac agaaagtgag aggttgtagc acattgccac cgtccagatc
     2761 tcgggtcatc tcaaacgatg gagccagccg cttctctgga ggcagcctcc tcacgactgg
     2821 aagctcaagg cgaaaacacg tggtgcaagc acagaagctg gcggacgtgg acagtgagct
     2881 ggctgccatg ctactgaccc atgcccggca gggcaagggg ccccaggacg tcagccgcga
     2941 gtcggatgcc acccgcaggc gtaagctgga gcggatgagg tctgtgcgcc tgcaggaggc
     3001 cgggggagac ttgggccggc gcgggacgag cgtgttggcg cagcagagcg tccgcacaca
     3061 gcacttgcgg gacctacagg tcatcgccgc ctaccgggaa cgcacgaagg ccgagagcat
     3121 cgccagcctg ctgagcctgg ccatcaccac ggagcacacg ctccacgcca cgctgggggt
     3181 cgccgagttc tttgagtttg tgcttaagaa cccccacaac acacagcaca cggtgactgt
     3241 ggagatcgac aaccccgagc tcagcgtcat cgtggacagt caggagtgga gggacttcaa
     3301 gggtgctgct ggcctgcaca caccggtgga ggaggacatg ttccacctgc gtggcagcct
     3361 ggccccccag ctctacctgc gcccccacga gaccgcccac gtccccttca agttccagag
     3421 cttctctgca gggcagctgg ccatggtgca ggcctctcct gggttgagca acgagaaggg
     3481 catggacgcc gtgtcacctt ggaagtccag cgcagtgccc actaaacacg ccaaggtctt
     3541 gttccgagcg agtggtggca agcccatcgc cgtgctctgc ctgactgtgg agctgcagcc
     3601 ccacgtggtg gaccaggtct tccgcttcta tcacccggag ctctccttcc tgaagaaggc
     3661 catccgcctg ccgccctggc acacatttcc aggtgctccg gtgggaatgc ttggtgagga
     3721 ccccccagtc catgttcgct gcagcgaccc gaacgtcatc tgtgagaccc agaatgtggg
     3781 ccccggggaa ccacgggaca tatttctgaa ggtggccagt ggtccaagcc cggagatcaa
     3841 agacttcttt gtcatcattt actcggatcg ctggctggcg acacccacac agacgtggca
     3901 ggtctacctc cactccctgc agcgcgtgga tgtctcctgc gtcgcaggcc agctgacccg
     3961 cctgtccctt gtccttcggg ggacacagac agtgaggaaa gtgagagctt tcacctctca
     4021 tccccaggag ctgaagacag accccaaagg tgtcttcgtg ctgccgcctc gtggggtgca
     4081 ggacctgcat gttggcgtga ggccccttag ggccggcagc cgctttgtcc atctcaacct
     4141 ggtggacgtg gattgccacc agctggtggc ctcctggctc gtgtgcctct gctgccgcca
     4201 gccgctcatc tccaaggcct ttgagatcat gttggctgcg ggcgaaggga agggtgtcaa
     4261 caagaggatc acctacacca acccctaccc ctcccggagg acattccacc tgcacagcga
     4321 ccacccggag ctgctgcggt tcagagagga ctccttccag gtcgggggtg gagagaccta
     4381 caccatcggc ttgcagtttg cgcctagtca gagagtgggt gaggaggaga tcctgatcta
     4441 catcaatgac catgaggaca aaaacgaaga ggcattttgc gtgaaggtca tctaccagtg
     4501 agggcttgag ggtgacgtcc ttcctgcggc acccagctgg ggcctgtctg tgcccctcct
     4561 gccctgcagg ctgtcctccc cgcctctctg cagcctttca cttcagtgcc cacctggctg
     4621 acctgtgcac ttggctgagg aagcagagac cgagcgctgg tcattttgta gtacctgcat
     4681 ccagcttagc tgctgctgac acccagcagg cctgggttcc gtgagcgcga actccgtggt
     4741 ggtgggtctg gctctggtgc tgccatctac gcatgtggga ccctcgttat cgctgttgct
     4801 caaaatgtat tttatgaatc atcctaaatg agaaaattat gtttttctta ctggattttg
     4861 tacaaacata atctattatt tgctatgcaa tattttatgc tggtattata tctgtttttt
     4921 aaattgttga acaaaatact aaacttttac acgtc
//
