LOCUS       NM_004885               1964 bp    mRNA    linear   PRI 08-FEB-2025
DEFINITION  Homo sapiens neuropeptide FF receptor 2 (NPFFR2), transcript
            variant 1, mRNA.
ACCESSION   NM_004885
VERSION     NM_004885.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 1964)
  AUTHORS   Jiang,H., Wang,L., Zhu,J. and Ping,Z.
  TITLE     NPFFR2 gene compound heterozygous variants associated with
            preeclampsia identified by whole-exome sequencing
  JOURNAL   Gene 854, 147108 (2023)
   PUBMED   36535464
  REMARK    GeneRIF: NPFFR2 gene compound heterozygous variants associated with
            preeclampsia identified by whole-exome sequencing.
            Erratum:[Gene. 2023 Dec 30;889:147759. doi:
            10.1016/j.gene.2023.147759.. PMID: 37696100]
REFERENCE   2  (bases 1 to 1964)
  AUTHORS   Ovejero-Benito,M.C., Munoz-Aceituno,E., Sabador,D., Almoguera,B.,
            Prieto-Perez,R., Hakonarson,H., Coto-Segura,P., Carretero,G.,
            Reolid,A., Llamas-Velasco,M., Abad-Santos,F. and Dauden,E.
  TITLE     Genome-wide association analysis of psoriasis patients treated with
            anti-TNF drugs
  JOURNAL   Exp Dermatol 29 (12), 1225-1232 (2020)
   PUBMED   33058233
  REMARK    GeneRIF: Genome-wide association analysis of psoriasis patients
            treated with anti-TNF drugs.
REFERENCE   3  (bases 1 to 1964)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 1964)
  AUTHORS   Roumeas,L., Humbert,J.P., Schneider,S., Doebelin,C., Bertin,I.,
            Schmitt,M., Bourguignon,J.J., Simonin,F. and Bihel,F.
  TITLE     Effects of systematic N-terminus deletions and benzoylations of
            endogenous RF-amide peptides on NPFF1R, NPFF2R, GPR10, GPR54 and
            GPR103
  JOURNAL   Peptides 71, 156-161 (2015)
   PUBMED   26211894
  REMARK    GeneRIF: Effects of systematic N-terminus deletions and
            benzoylations of endogenous RF-amide peptides on NPFF1R, NPFF2R,
            GPR10, GPR54 and GPR103.
REFERENCE   5  (bases 1 to 1964)
  AUTHORS   Bray,L., Froment,C., Pardo,P., Candotto,C., Burlet-Schiltz,O.,
            Zajac,J.M., Mollereau,C. and Mouledous,L.
  TITLE     Identification and functional characterization of the
            phosphorylation sites of the neuropeptide FF2 receptor
  JOURNAL   J Biol Chem 289 (49), 33754-33766 (2014)
   PUBMED   25326382
  REMARK    GeneRIF: The NPFF2 neuropeptide FF receptor phosphorylation sites
            have been mapped and their role identified in receptor regulation.
REFERENCE   6  (bases 1 to 1964)
  AUTHORS   Engstrom,M., Brandt,A., Wurster,S., Savola,J.M. and Panula,P.
  TITLE     Prolactin releasing peptide has high affinity and efficacy at
            neuropeptide FF2 receptors
  JOURNAL   J Pharmacol Exp Ther 305 (3), 825-832 (2003)
   PUBMED   12606605
REFERENCE   7  (bases 1 to 1964)
  AUTHORS   Bonini,J.A., Jones,K.A., Adham,N., Forray,C., Artymyshyn,R.,
            Durkin,M.M., Smith,K.E., Tamm,J.A., Boteju,L.W., Lakhlani,P.P.,
            Raddatz,R., Yao,W.J., Ogozalek,K.L., Boyle,N., Kouranova,E.V.,
            Quan,Y., Vaysse,P.J., Wetzel,J.M., Branchek,T.A., Gerald,C. and
            Borowsky,B.
  TITLE     Identification and characterization of two G protein-coupled
            receptors for neuropeptide FF
  JOURNAL   J Biol Chem 275 (50), 39324-39331 (2000)
   PUBMED   11024015
REFERENCE   8  (bases 1 to 1964)
  AUTHORS   Elshourbagy,N.A., Ames,R.S., Fitzgerald,L.R., Foley,J.J.,
            Chambers,J.K., Szekeres,P.G., Evans,N.A., Schmidt,D.B.,
            Buckley,P.T., Dytko,G.M., Murdock,P.R., Milligan,G., Groarke,D.A.,
            Tan,K.B., Shabon,U., Nuthulaganti,P., Wang,D.Y., Wilson,S.,
            Bergsma,D.J. and Sarau,H.M.
  TITLE     Receptor for the pain modulatory neuropeptides FF and AF is an
            orphan G protein-coupled receptor
  JOURNAL   J Biol Chem 275 (34), 25965-25971 (2000)
   PUBMED   10851242
REFERENCE   9  (bases 1 to 1964)
  AUTHORS   Parker,R.M., Copeland,N.G., Eyre,H.J., Liu,M., Gilbert,D.J.,
            Crawford,J., Couzens,M., Sutherland,G.R., Jenkins,N.A. and
            Herzog,H.
  TITLE     Molecular cloning and characterisation of GPR74 a novel G-protein
            coupled receptor closest related to the Y-receptor family
  JOURNAL   Brain Res Mol Brain Res 77 (2), 199-208 (2000)
   PUBMED   10837915
REFERENCE   10 (bases 1 to 1964)
  AUTHORS   Cikos,S., Gregor,P. and Koppel,J.
  TITLE     Sequence and tissue distribution of a novel G-protein-coupled
            receptor expressed prominently in human placenta
  JOURNAL   Biochem Biophys Res Commun 256 (2), 352-356 (1999)
   PUBMED   10079187
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC106898.5 and AC106051.3.
            
            On Jun 27, 2020 this sequence version replaced NM_004885.2.
            
            Summary: This gene encodes a member of a subfamily of
            G-protein-coupled neuropeptide receptors. This protein is activated
            by the neuropeptides A-18-amide (NPAF) and F-8-amide (NPFF) and may
            function in pain modulation and regulation of the opioid system.
            Alternative splicing results in multiple transcript variants.
            [provided by RefSeq, Jan 2009].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF119815.1, AK308069.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145743, SAMEA2162568
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000308744.12/ ENSP00000307822.7
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-201               AC106898.5         75738-75938
            202-536             AC106051.3         2477-2811
            537-636             AC106051.3         11932-12031
            637-1964            AC106051.3         20870-22197
FEATURES             Location/Qualifiers
     source          1..1964
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="4"
                     /map="4q13.3"
     gene            1..1964
                     /gene="NPFFR2"
                     /gene_synonym="GPR74; HLWAR77; NPFF2; NPGPR"
                     /note="neuropeptide FF receptor 2"
                     /db_xref="GeneID:10886"
                     /db_xref="HGNC:HGNC:4525"
                     /db_xref="MIM:607449"
     exon            1..201
                     /gene="NPFFR2"
                     /gene_synonym="GPR74; HLWAR77; NPFF2; NPGPR"
                     /inference="alignment:Splign:2.1.0"
     exon            202..536
                     /gene="NPFFR2"
                     /gene_synonym="GPR74; HLWAR77; NPFF2; NPGPR"
                     /inference="alignment:Splign:2.1.0"
     CDS             209..1471
                     /gene="NPFFR2"
                     /gene_synonym="GPR74; HLWAR77; NPFF2; NPGPR"
                     /note="isoform 1 is encoded by transcript variant 1;
                     neuropeptide FF 2; G-protein coupled receptor 74;
                     G-protein coupled receptor HLWAR77; neuropeptide G-protein
                     coupled receptor"
                     /codon_start=1
                     /product="neuropeptide FF receptor 2 isoform 1"
                     /protein_id="NP_004876.3"
                     /db_xref="CCDS:CCDS3552.1"
                     /db_xref="GeneID:10886"
                     /db_xref="HGNC:HGNC:4525"
                     /db_xref="MIM:607449"
                     /translation="MNEKWDTNSSENWHPIWNVNDTKHHLYSDINITYVNYYLHQPQV
                     AAIFIISYFLIFFLCMMGNTVVCFIVMRNKHMHTVTNLFILNLAISDLLVGIFCMPIT
                     LLDNIIAGWPFGNTMCKISGLVQGISVAASVFTLVAIAVDRFQCVVYPFKPKLTIKTA
                     FVIIMIIWVLAITIMSPSAVMLHVQEEKYYRVRLNSQNKTSPVYWCREDWPNQEMRKI
                     YTTVLFANIYLAPLSLIVIMYGRIGISLFRAAVPHTGRKNQEQWHVVSRKKQKIIKML
                     LIVALLFILSWLPLWTLMMLSDYADLSPNELQIINIYIYPFAHWLAFGNSSVNPIIYG
                     FFNENFRRGFQEAFQLQLCQKRAKPMEAYALKAKSHVLINTSNQLVQESTFQNPHGET
                     LLYRKSAEKPQQELVMEELKETTNSSEI"
     exon            537..636
                     /gene="NPFFR2"
                     /gene_synonym="GPR74; HLWAR77; NPFF2; NPGPR"
                     /inference="alignment:Splign:2.1.0"
     exon            637..1964
                     /gene="NPFFR2"
                     /gene_synonym="GPR74; HLWAR77; NPFF2; NPGPR"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1695..1700
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NPFFR2"
                     /gene_synonym="GPR74; HLWAR77; NPFF2; NPGPR"
                     /note="hexamer: AATAAA"
     polyA_site      1712
                     /gene="NPFFR2"
                     /gene_synonym="GPR74; HLWAR77; NPFF2; NPGPR"
     regulatory      1944..1949
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NPFFR2"
                     /gene_synonym="GPR74; HLWAR77; NPFF2; NPGPR"
                     /note="hexamer: AATACA"
     polyA_site      1964
                     /gene="NPFFR2"
                     /gene_synonym="GPR74; HLWAR77; NPFF2; NPGPR"
                     /note="major polyA site"
ORIGIN      
        1 actcagcgtc cagcagcgcg gcgggccagc ctggagcgga agcctggagt ggagcaggca
       61 gtccgcgggg gacagacgtc ggctgggatt gagccggcag actgcgaaaa gtagctggag
      121 ccggagcagg gacagaacct gttgctgcag acgggcttgg tggattctgg ttcctgccgc
      181 cgacagggct cgccgggaga ggttcatcat gaatgagaaa tgggacacaa actcttcaga
      241 aaactggcat cccatctgga atgtcaatga cacaaagcat catctgtact cagatattaa
      301 tattacctat gtgaactact atcttcacca gcctcaagtg gcagcaatct tcattatttc
      361 ctactttctg atcttctttt tgtgcatgat gggaaatact gtggtttgct ttattgtaat
      421 gaggaacaaa catatgcaca cagtcactaa tctcttcatc ttaaacctgg ccataagtga
      481 tttactagtt ggcatattct gcatgcctat aacactgctg gacaatatta tagcaggatg
      541 gccatttgga aacacgatgt gcaagatcag tggattggtc cagggaatat ctgtcgcagc
      601 ttcagtcttt acgttagttg caattgctgt agataggttc cagtgtgtgg tctacccttt
      661 taaaccaaag ctcactatca agacagcgtt tgtcattatt atgatcatct gggtcctagc
      721 catcaccatt atgtctccat ctgcagtaat gttacatgtg caagaagaaa aatattaccg
      781 agtgagactc aactcccaga ataaaaccag tccagtctac tggtgccggg aagactggcc
      841 aaatcaggaa atgaggaaga tctacaccac tgtgctgttt gccaacatct acctggctcc
      901 cctctccctc attgtcatca tgtatggaag gattggaatt tcactcttca gggctgcagt
      961 tcctcacaca ggcaggaaga accaggagca gtggcacgtg gtgtccagga agaagcagaa
     1021 gatcattaag atgctcctga ttgtggccct gctttttatt ctctcatggc tgcccctgtg
     1081 gactctaatg atgctctcag actacgctga cctttctcca aatgaactgc agatcatcaa
     1141 catctacatc tacccttttg cacactggct ggcattcggc aacagcagtg tcaatcccat
     1201 catttatggt ttcttcaacg agaatttccg ccgtggtttc caagaagctt tccagctcca
     1261 gctctgccaa aaaagagcaa agcctatgga agcttatgcc ctaaaagcta aaagccatgt
     1321 gctcataaac acatctaatc agcttgtcca ggaatctaca tttcaaaacc ctcatgggga
     1381 aaccttgctt tataggaaaa gtgctgaaaa accccaacag gaattagtga tggaagaatt
     1441 aaaagaaact actaacagca gtgagattta aaaagagcta gtgtgataat cctaactcta
     1501 ctacgcatta tatatttaaa tccattgctt tttgtggctt tgcacttcaa atttttcaaa
     1561 gaatgttcta aataaaacat ttactgaaag ccctctctgg caaaaaaatt aaaaataaac
     1621 aaaaatggtc ataagatcat aaacaatctt atgttgtata aaaatacgta gagtgactta
     1681 gacatgtttg catgaataaa tatatttcta gagaacagtt tacaaagcct catctttcca
     1741 aacttaacca tttgtgtatg cgtcaaatca agcctgcacg cgtgcgtgca tgtgtgtgtg
     1801 tattttcccc aaatggtgat gatgagcagt gctttgcatg aaactagatt ttatcaattt
     1861 ctcctttgat tttggattta aaattttaga ttacaagact attactctgc ttatgtttat
     1921 aaactgtact tggttttcta agaaatacat tcgatcacat gaaa
//
