LOCUS       NM_022146               9249 bp    mRNA    linear   PRI 08-JUN-2024
DEFINITION  Homo sapiens neuropeptide FF receptor 1 (NPFFR1), mRNA.
ACCESSION   NM_022146
VERSION     NM_022146.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9249)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   2  (bases 1 to 9249)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   3  (bases 1 to 9249)
  AUTHORS   Badgandi,H.B., Hwang,S.H., Shimada,I.S., Loriot,E. and
            Mukhopadhyay,S.
  TITLE     Tubby family proteins are adapters for ciliary trafficking of
            integral membrane proteins
  JOURNAL   J Cell Biol 216 (3), 743-760 (2017)
   PUBMED   28154160
REFERENCE   4  (bases 1 to 9249)
  AUTHORS   Roumeas,L., Humbert,J.P., Schneider,S., Doebelin,C., Bertin,I.,
            Schmitt,M., Bourguignon,J.J., Simonin,F. and Bihel,F.
  TITLE     Effects of systematic N-terminus deletions and benzoylations of
            endogenous RF-amide peptides on NPFF1R, NPFF2R, GPR10, GPR54 and
            GPR103
  JOURNAL   Peptides 71, 156-161 (2015)
   PUBMED   26211894
  REMARK    GeneRIF: Effects of systematic N-terminus deletions and
            benzoylations of endogenous RF-amide peptides on NPFF1R, NPFF2R,
            GPR10, GPR54 and GPR103.
REFERENCE   5  (bases 1 to 9249)
  AUTHORS   Lima,C.J., Cardoso,S.C., Lemos,E.F., Zingler,E., Capanema,C.,
            Menezes,L.D., Vogado,G., Dos Santos,B.T., de Moraes,O.L.,
            Duarte,E.F., de Brito,V.N., Latronico,A.C. and Lofrano-Porto,A.
  TITLE     Mutational analysis of the genes encoding RFamide-related
            peptide-3, the human orthologue of gonadotrophin-inhibitory
            hormone, and its receptor (GPR147) in patients with
            gonadotrophin-releasing hormone-dependent pubertal disorders
  JOURNAL   J Neuroendocrinol 26 (11), 817-824 (2014)
   PUBMED   25180599
  REMARK    GeneRIF: RFRP-3/GPR147 may play secondary, modulatory roles on the
            regulation of pubertal development; a restraining modulatory effect
            of the NPVF p.I71_K72 variant on the activation of the
            gonadotrophic axis cannot be ruled out
REFERENCE   6  (bases 1 to 9249)
  AUTHORS   Ubuka,T., Morgan,K., Pawson,A.J., Osugi,T., Chowdhury,V.S.,
            Minakata,H., Tsutsui,K., Millar,R.P. and Bentley,G.E.
  TITLE     Identification of human GnIH homologs, RFRP-1 and RFRP-3, and the
            cognate receptor, GPR147 in the human hypothalamic pituitary axis
  JOURNAL   PLoS One 4 (12), e8400 (2009)
   PUBMED   20027225
  REMARK    GeneRIF: GnIH homologs in the human hypothalamus and were
            identified and human GnIH receptor (GPR147) mRNA expression in the
            hypothalamus as well as in the pituitary, was characterized.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 9249)
  AUTHORS   Dowal,L., Provitera,P. and Scarlata,S.
  TITLE     Stable association between G alpha(q) and phospholipase C beta 1 in
            living cells
  JOURNAL   J Biol Chem 281 (33), 23999-24014 (2006)
   PUBMED   16754659
REFERENCE   8  (bases 1 to 9249)
  AUTHORS   Grupe,A., Li,Y., Rowland,C., Nowotny,P., Hinrichs,A.L., Smemo,S.,
            Kauwe,J.S., Maxwell,T.J., Cherny,S., Doil,L., Tacey,K., van
            Luchene,R., Myers,A., Wavrant-De Vrieze,F., Kaleem,M.,
            Hollingworth,P., Jehu,L., Foy,C., Archer,N., Hamilton,G.,
            Holmans,P., Morris,C.M., Catanese,J., Sninsky,J., White,T.J.,
            Powell,J., Hardy,J., O'Donovan,M., Lovestone,S., Jones,L.,
            Morris,J.C., Thal,L., Owen,M., Williams,J. and Goate,A.
  TITLE     A scan of chromosome 10 identifies a novel locus showing strong
            association with late-onset Alzheimer disease
  JOURNAL   Am J Hum Genet 78 (1), 78-88 (2006)
   PUBMED   16385451
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   9  (bases 1 to 9249)
  AUTHORS   Bonini,J.A., Jones,K.A., Adham,N., Forray,C., Artymyshyn,R.,
            Durkin,M.M., Smith,K.E., Tamm,J.A., Boteju,L.W., Lakhlani,P.P.,
            Raddatz,R., Yao,W.J., Ogozalek,K.L., Boyle,N., Kouranova,E.V.,
            Quan,Y., Vaysse,P.J., Wetzel,J.M., Branchek,T.A., Gerald,C. and
            Borowsky,B.
  TITLE     Identification and characterization of two G protein-coupled
            receptors for neuropeptide FF
  JOURNAL   J Biol Chem 275 (50), 39324-39331 (2000)
   PUBMED   11024015
REFERENCE   10 (bases 1 to 9249)
  AUTHORS   Hinuma,S., Shintani,Y., Fukusumi,S., Iijima,N., Matsumoto,Y.,
            Hosoya,M., Fujii,R., Watanabe,T., Kikuchi,K., Terao,Y., Yano,T.,
            Yamamoto,T., Kawamata,Y., Habata,Y., Asada,M., Kitada,C.,
            Kurokawa,T., Onda,H., Nishimura,O., Tanaka,M., Ibata,Y. and
            Fujino,M.
  TITLE     New neuropeptides containing carboxy-terminal RFamide and their
            receptor in mammals
  JOURNAL   Nat Cell Biol 2 (10), 703-708 (2000)
   PUBMED   11025660
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL355138.19 and EU432128.1.
            
            On May 17, 2019 this sequence version replaced NM_022146.4.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            RNAseq introns :: mixed sample support SAMEA1965299, SAMEA1966682
                              [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000277942.7/ ENSP00000277942.5
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-335               AL355138.19        41335-41669         c
            336-1621            EU432128.1         8-1293
            1622-9249           AL355138.19        4994-12621          c
FEATURES             Location/Qualifiers
     source          1..9249
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q22.1"
     gene            1..9249
                     /gene="NPFFR1"
                     /gene_synonym="GPR147; NPFF1; NPFF1R1; OT7T022"
                     /note="neuropeptide FF receptor 1"
                     /db_xref="GeneID:64106"
                     /db_xref="HGNC:HGNC:17425"
                     /db_xref="MIM:607448"
     exon            1..335
                     /gene="NPFFR1"
                     /gene_synonym="GPR147; NPFF1; NPFF1R1; OT7T022"
                     /inference="alignment:Splign:2.1.0"
     CDS             329..1621
                     /gene="NPFFR1"
                     /gene_synonym="GPR147; NPFF1; NPFF1R1; OT7T022"
                     /note="neuropeptide FF 1; G-protein coupled receptor 147;
                     RFamide-related peptide receptor OT7T022"
                     /codon_start=1
                     /product="neuropeptide FF receptor 1"
                     /protein_id="NP_071429.1"
                     /db_xref="CCDS:CCDS53539.1"
                     /db_xref="GeneID:64106"
                     /db_xref="HGNC:HGNC:17425"
                     /db_xref="MIM:607448"
                     /translation="MEGEPSQPPNSSWPLSQNGTNTEATPATNLTFSSYYQHTSPVAA
                     MFIVAYALIFLLCMVGNTLVCFIVLKNRHMHTVTNMFILNLAVSDLLVGIFCMPTTLV
                     DNLITGWPFDNATCKMSGLVQGMSVSASVFTLVAIAVERFRCIVHPFREKLTLRKALV
                     TIAVIWALALLIMCPSAVTLTVTREEHHFMVDARNRSYPLYSCWEAWPEKGMRRVYTT
                     VLFSHIYLAPLALIVVMYARIARKLCQAPGPAPGGEEAADPRASRRRARVVHMLVMVA
                     LFFTLSWLPLWALLLLIDYGQLSAPQLHLVTVYAFPFAHWLAFFNSSANPIIYGYFNE
                     NFRRGFQAAFRARLCPRPSGSHKEAYSERPGGLLHRRVFVVVRPSDSGLPSESGPSSG
                     APRPGRLPLRNGRVAHHGLPREGPGCSHLPLTIPAWDI"
     exon            336..650
                     /gene="NPFFR1"
                     /gene_synonym="GPR147; NPFF1; NPFF1R1; OT7T022"
                     /inference="alignment:Splign:2.1.0"
     exon            651..750
                     /gene="NPFFR1"
                     /gene_synonym="GPR147; NPFF1; NPFF1R1; OT7T022"
                     /inference="alignment:Splign:2.1.0"
     exon            751..9249
                     /gene="NPFFR1"
                     /gene_synonym="GPR147; NPFF1; NPFF1R1; OT7T022"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9230..9235
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NPFFR1"
                     /gene_synonym="GPR147; NPFF1; NPFF1R1; OT7T022"
                     /note="hexamer: AATAAA"
     polyA_site      9249
                     /gene="NPFFR1"
                     /gene_synonym="GPR147; NPFF1; NPFF1R1; OT7T022"
                     /note="major polyA site"
ORIGIN      
        1 aggtgcgcgg gtgccatgac gactgcgcct cacgagcccc ctccttcccc tggtcgggag
       61 gcgaggggcg gcgggcggct gagccctgcg ggcagcagcg ctgcgcggga gccagtcagc
      121 ccgaggggag ggaaaccggc cagggcccgg ggcctgggag cgcacgcccc ctgctcacct
      181 ctgcccggac cgaccggacc tgcggagcgc ggaggcaggg gcgcagcccg caaccaagtg
      241 cccggagacc gtccctctgc ccgctggccc gaaggggccc gctgcctccc gccatcggag
      301 accgccggag cccgcaactg ggggcatcat ggagggggag ccctcccagc ctcccaacag
      361 cagttggccc ctaagtcaga atgggactaa cactgaggcc accccggcta caaacctcac
      421 cttctcctcc tactatcagc acacctcccc tgtggcggcc atgttcattg tggcctatgc
      481 gctcatcttc ctgctctgca tggtgggcaa caccctggtc tgtttcatcg tgctcaagaa
      541 ccggcacatg catactgtca ccaacatgtt catcctcaac ctggctgtca gtgacctgct
      601 ggtgggcatc ttctgcatgc ccaccaccct tgtggacaac ctcatcactg ggtggccctt
      661 cgacaatgcc acatgcaaga tgagcggctt ggtgcagggc atgtctgtgt cggcttccgt
      721 tttcacactg gtggccattg ctgtggaaag gttccgctgc atcgtgcacc ctttccgcga
      781 gaagctgacc ctgcggaagg cgctcgtcac catcgccgtc atctgggccc tggcgctgct
      841 catcatgtgt ccctcggccg tcacgctgac cgtcacccgt gaggagcacc acttcatggt
      901 ggacgcccgc aaccgctcct acccgctcta ctcctgctgg gaggcctggc ccgagaaggg
      961 catgcgcagg gtctacacca ctgtgctctt ctcgcacatc tacctggcgc cgctggcgct
     1021 catcgtggtc atgtacgccc gcatcgcgcg caagctctgc caggccccgg gcccggcccc
     1081 cgggggcgag gaggctgcgg acccgcgagc atcgcggcgc agagcgcgcg tggtgcacat
     1141 gctggtcatg gtggcgctgt tcttcacgct gtcctggctg ccgctctggg cgctgctgct
     1201 gctcatcgac tacgggcagc tcagcgcgcc gcagctgcac ctggtcaccg tctacgcctt
     1261 ccccttcgcg cactggctgg ccttcttcaa cagcagcgcc aaccccatca tctacggcta
     1321 cttcaacgag aacttccgcc gcggcttcca ggccgccttc cgcgcccgcc tctgcccgcg
     1381 cccgtcgggg agccacaagg aggcctactc cgagcggccc ggcgggcttc tgcacaggcg
     1441 ggtcttcgtg gtggtgcggc ccagcgactc cgggctgccc tctgagtcgg gccctagcag
     1501 tggggccccc aggcccggcc gcctcccgct gcggaatggg cgggtggctc accacggctt
     1561 gcccagggaa gggcctggct gctcccacct gcccctcacc attccagcct gggatatctg
     1621 agggggtcca gggagggcgg gacgctgcct cagggccccg attggacacg agatacatgc
     1681 aggcgatagc ggcctggtgt ggttaggtga ggatgcgtgg tccctccctc tccagagcca
     1741 ggcagtggtg ggcagctgct ggtgatgtct tctctgctgc cttcaccctc agtagaggca
     1801 aatgcggggg acgggaagga accaacttgc cctgtgtggt gggcttgaca aacatcatct
     1861 cactgaattg tcacaacgct ttggagatag gttgtcttct cttccttcta cagatgcaaa
     1921 aactgaggcc tggagagatg tgactcaccc aagtttctca ggtggaaatg gcatgtgaag
     1981 ctatctgact ccaatacctg tgtggttttt atcatctcca gctggctcca agagggcccg
     2041 gtaaggtaga acactgtgaa ctgagtaagt cctaattagg cagctaggaa gaagcgaaaa
     2101 gcaaagccgg cctgtccctt gatgctttgt gtccatgtga gggagtccct catgttccgt
     2161 tactgaagcc actccccttg atccttttct agaagaacac ggtgtgcccc cgctgccggc
     2221 tctaatgtgt tggtttgtct tgtttgttcc aagaagttcc aagaagccat tgtcctcaga
     2281 ggacggcagc ttcagaacga gccttgggga ggctgcctca gttgcagggc actctaggtc
     2341 ttgccctctg ggtctgagct tagagaacag aggagggact cccagaccaa ggcaagaact
     2401 tacaatgcca cagacattgt aatgctgcag ggccacagga ggtgtccttt tacgtactca
     2461 tagacaacga ggaaggggaa gcacggtatg cattttccac attgggaaac acatctgctc
     2521 actggggctg caggtacaaa gccttgccca aggtctctct cctccctgct tcctgggagg
     2581 gagaagttgg tcaactgaag ctgcagccgg agtgccattt ggagactcct gaaggacaca
     2641 cactaaactc ttccccagca caaatgactt gtgtactcta aggaggtacc aggagagccc
     2701 tgtttactct ggctttcccc caacacccct gcatcaactt gaagaaatgt ggcttctttc
     2761 caggagacgg gagcacatgg gattgccttg gagggtcctt tcacaggccc cttcatcttg
     2821 cacttaaatg gacatttccc cccacctctg tagaaaacac ggtgttgcta gaaatttagt
     2881 aaacaaaaaa gaaacgtttc caggggtcgg ggctctgagg ctctgttccc ttaaccgaca
     2941 cacagtgact gtttactgtt tgctccactc taagttccag gtttaggggc agaagaaaat
     3001 tcctaagcag ttgtacaata cattctccaa gcatgtcccc agacaccgtc tcatttaatc
     3061 catagtaatg aggttgaagg agattgaatg ttattgtcat ttcacaaagt gactgaagct
     3121 caaaggggcc aagtttccag ggccacagag gggcaggatt tagattaaaa tttgtcttct
     3181 gattgttctt cccaccccac atgctgcctt tcaaggaagg tggggatgac gggagagctg
     3241 gtaatttccc caaaggcttt tttgcctaac agtgaaccag aatgcccact cctggtgccc
     3301 aagctcttgg tctgggaaag aaatctattg tgtttactct cgtaatgtcc agcctgagtt
     3361 gttcttgccc tggtgctgag caggctgcca agagccatca tgaccttgtc ctggttctgg
     3421 gggtgatgga tgagggtctg agagagagta tgccctcctg cccctcagtg gtattagctg
     3481 gtgccatgca tttctggtac tgtatcagtg tcatgtgtca tatgggccag agagttccca
     3541 gctggttctg ggctatttgt tgtgaagaag tctgactcca ggctctaaaa gtaagccaga
     3601 gaaagggccg tctcaaaccc aaaatgccta tccctatggc tgggtgggaa ttgtctgaag
     3661 acaccacctg gtgtctcctc tggggtgtgt ttctccttta gcatagcctc cccaagtaca
     3721 agatcagcat ttgaggctgg gggcgggtag ggataccaaa gtactcactc aagccaacct
     3781 ttccatcaca gcccctttgt cttcccccaa aagatgtcat gctttgaaag gttataactg
     3841 cccaaaaaac tccttcagct ctcgctgaag gttacaatta acatattttg ctgttatttt
     3901 tgtttcttct aaaaaatcaa tcagaggcta tacctgccca ttcagatctg agtgataaca
     3961 ggcatgacaa tgctttaatg aagcagtgag acattgtggg tatgtgggga gttcgtttct
     4021 ttgtggatgt ggtaaaatat aaataatata gaatttacca ttttggccat ttttaagtgt
     4081 acaggactgg aattaaatat attcacattg ttgtgtaacc attaccacta tctattttca
     4141 gaatttttta ttatcccaaa ctgcaactct gtatctataa acaatatcac cgcatcccct
     4201 ctcctcctag cccctggtaa ctactattct actttctgtc tgtgaatttg gccactctag
     4261 gtacttctat acaagaggaa tcatacaata ctatcctttt tgactggctt ctttcactta
     4321 gcacaatgtc ttcaaggttc atccatgttg tggcatgtgt cagaatttct ttcattttaa
     4381 aagctgaata atatcccatt gtctagatag tcctcatttt gttcattcat ccatcagtgg
     4441 acacttaggt tgtttctatc ttttggctac tgtgaataat gtatggagtt tttagatgct
     4501 agaagtagtt cctgacaata gtgcctgtgt gaatcctgag gacagagacc ccaggttggg
     4561 aaccagggtc tctcccaact taccctttat ggctgacaaa acagaggctt ccaggacagc
     4621 tgtgatgtgt ctcacttccc tgtgcccacc ccagctgctc tgggcttctg ctgggcttta
     4681 cttcctgact agagtggcta gaaaattgta tgtttcctct acaagatgtt tttatgaagc
     4741 tattttataa ttatagattt attatcctca ctggattcct atgttctgac acctggaccc
     4801 tgacggggcc ttgtgcagcc taggcgccca cgggcaagtg gtgtggacca ttcctgacac
     4861 gccatcctcc acctccaggt tggctgggtt tcctaaggtg actgcccttt cataagcggc
     4921 aagttcggag atgaacggca tgcgtgatgg gggaaaaggg ccagaggcct gttgacactg
     4981 agcatcccca cccagagggc ctcctggagg gactctgtgt cctgtcctgc catgtgcggc
     5041 atgagtggtc accctcattg tggctctcca ctgatgaatg gcctcttgac tttaccaaga
     5101 ccaggcagca cccaacagac caatgtgagc tggaggcgga tgccgggcct gcaccccaca
     5161 aaccacagtc tgcccagccc tgtgctgagg agtctctctt ggggcctgga ggactcatga
     5221 gctgatctgc attggaatgg agggcgaagt tggcaaagga agatgagagc ccccctatca
     5281 ttagtctact gtgtattcat ccaaaaagca aggcgagagc caagcccaga gctggatgga
     5341 caaaggattg ctattaagct ctgtaccttt ttgcacccgt ttcctcagct cacccctcag
     5401 aagtgtcact gatgaaagat agatgagaag gaggcggtct aaatgggctt cattacaatg
     5461 aacaatgttg aatgaacaat acattccttg ttgaataacc caattattta tgatcatggc
     5521 attttattgt tgtttttctc tctctctaca aaagctctac ataatcattg tggaaatttt
     5581 acaagatttc aataagcaaa gacaagaaaa tgaaaagcac ccaaaatcct accgcaagag
     5641 acaagtatag cgtccttttc tgtgcatata tctctcaata tatttaacat tcttggaaca
     5701 gctttattga ggcagaactt gaatatgaaa aaattcaccc gtcttaagtg tactgcttga
     5761 cgagttttaa cagttgtata tagttatgtc cctaccacac tgaagatatt ttcatcatcc
     5821 taaaatgttt ccttgtgcta atttatagtc agtcctttcc cacgctcctg attccagaaa
     5881 acgatgatct gatttcagaa tgtcatgtag atggaatcag acagtttgca gccgagtctg
     5941 gtttctttca cttaaaatga tgtttgcaag attcattcat gctgtagcat gtatcagtag
     6001 tttttccttt tgattgctga gtactattcc actgtatgaa tagatcacat tttgtttatc
     6061 cattcatcag ttgatagaca tttggttgtc accagttttt ggctattatt aataatgttg
     6121 ccattaacat tcatgtacaa gtccttatat gtatgtgtat gtatgttttc catttttctt
     6181 gggtagatac ctaggatcga gattggtagg ttgcatggtc tgtgtaaaat ttaactgtat
     6241 aagaaactgc caaagtgctt tccaaagtcg ctctaccatt ttgtactccc atcagcagtg
     6301 tatgaatgtt ccagttgttc tacatcctgg ccaacactta gcagtattag ccttttgaaa
     6361 atttccaggc caggtgtggt ggctcacgtc tgtaatccca gcactttggg aggctgaggt
     6421 gcgtagatca cctgaggtca ggagttcaag accagcctgg ccaatatggg gaaaccccat
     6481 ctctactaaa aacacaaaaa ttagccaggc atggtggcac acacctgtag tcccagctac
     6541 ttgggaggct gagacacgag aatcacttga acctaggagg cagaggatgc agtgagccaa
     6601 aattgcgcca ctgcactcca acctgggtga cagagcaaga ctgtctcaaa aaaaaaaaaa
     6661 aaaagaaaga aagaaagaag aaaagaaaat ttccctgatg actagtgatg ctgggcatct
     6721 ttttgtgtgc ttactgcatt cttatatctt ttattgtaaa gtatcttcaa atctttcttt
     6781 taaaaacatg attttggctg ggtgcggtgg ctcacacctg taatcccagc actttgggag
     6841 gccaaggtgg gcagatcacc tgaggtcagg agttcgagac cagcctggtc aacgtgatga
     6901 aaccccatct ctaccaaaaa cacaaaaatt agccaggtgt ggtgatgtgc gcctgtaatc
     6961 ccagctactt gggagactga ggcaggagaa tcacttgaac ctgggaggca gaggtttcag
     7021 tgagccaaga tggcgccact gcactccagc ctaggtgaca gagtgagatt ccatctcaaa
     7081 taataataat aataataata ttttattgta gagactttag cacaaaaact tgccctaact
     7141 ctaagggatt tttttgtgaa tataattttt ttttttttga gacagagtct tgcttttgtt
     7201 gcccaggctg gagtgcaatg acgtgatttt ggctcactgc aacctttgcc tcctaggttc
     7261 aagcgattct cctgcctcag cctcctgagt agctgggatt acaggcatct gccaccacac
     7321 ccagctaatt tttgtatttt taatagagat gggattttac catgttggcc aggctggtct
     7381 tgaactcctg acctcagatg atctgcccgc ctcggcctcc caaagtgctg ggattacagg
     7441 cgcgagccac catgctccac caatgatttt taaatccatt aagaatgggt ctaaagcaag
     7501 gtaagaggat atagtgtgat agaagtagga gggctatttg aacagatggt taggaatggc
     7561 ctctgtgaag aggtgatgtt tgagtacaga tatgaagtga gagagggagc catgtgacta
     7621 gctggggaag aaacaaaccc agacagagag agcatcatgt acacaggttc taagggagga
     7681 gtgggataac tgaaaaagga attagtagat atcaaaatgc tattgaattt caataattaa
     7741 atttctggtt aactcaaaac atgtattcag tgtctattaa aagccaggca taggccaggc
     7801 atgggggctc atgcctgtaa tcccagcact ttgggaggcc gaggtgggtc gatcacttga
     7861 ggtcaggagt tcgagaccag cctggctaac atggtgaaac cccatctcta ttaaaaatac
     7921 aaaaattagc caagcatgat ggcaggcacc tgtaatccca gctacttggg aggctgaggg
     7981 aggggaattg cttgaacctg ggaggcggag tttgcagtaa gccaagatag cgccactgta
     8041 ctctatcctg ggcaacagag tgagactcca tctcaaaaaa aaaaaaaaaa aacaaaaaac
     8101 aaaaaaaaaa cgccaggcat agtactacgt actttgtgtc tgttatttat ttctcacagt
     8161 ggaccataag ctccatgagg acaggaacta tgtctatttc acttaccttt gtggattcag
     8221 gtaggatatt gcctggtgca taggaggcac tcaataaatg tctgttgaat gaatgaagta
     8281 attaactaag caggtattta ttattattcc catacaatag attggaaaag caaggctcag
     8341 agaagtaaag taacttactg gaggttatat aaccaggagg tcctgaaggt gagactcaaa
     8401 ccttagtcta tctgaccaca aagtccaggt ttttgccatt ccccaagctg ctgtggtctc
     8461 tccttcctac atctgggaaa ctgaggttca gagtaaaaga aagagccttg atggaggtct
     8521 cccataatcc taaaaataaa accagccagc cacaggctcc aggctgctgt tttggccatg
     8581 agacacattt gcatcacttt caatggcttc caaagatttg ggatagaagg aataccagca
     8641 gtttaggtaa aaggtttaaa tttagtttga ctcaatacca catcaagccc tcaaagcagc
     8701 cacgggctcc cctgcactgt ggtcactgaa ggagcaaaag ctgctgaact ggctgtagcg
     8761 tcctgccttg agttgtttgt gttctcctgc tagctgcaat gcccaggtct ttgaatcctg
     8821 tcaccaataa atgatgctgt tgtcatgtgc aagcaaccaa attaatgtgg gtggaaaaac
     8881 ccaagcttag tgatttgggc aaattagttt atctgtcaca tagccagtgg tggtgttaaa
     8941 tcactctctg tttgaggagt aatcactctc cccccatcac caccctgtct tcctttcggt
     9001 gtggaagtta gaacagccaa gtcaatccgt ttcagtgact gagcgatcta catttctgtg
     9061 ttttgactgc cccccaggag gagtatgttg tgtagtgcat tttgtttgtc catagtgctg
     9121 tctcgtggtc ctccgaagcc acctctaatg atcataattg cctcttttat tcttctctag
     9181 tccgtgtctt tgcaaaatga ttctgttacc tatgctataa attatggaaa ataaactcat
     9241 tatgaaaga
//
