LOCUS       NM_006310               4309 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens aminopeptidase puromycin sensitive (NPEPPS),
            transcript variant 1, mRNA.
ACCESSION   NM_006310 XM_001128588
VERSION     NM_006310.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Huang,B., Xiong,X., Zhang,L., Liu,X., Wang,Y., Gong,X., Sang,Q.,
            Lu,Y., Qu,H., Zheng,H. and Zheng,Y.
  TITLE     PSA controls hepatic lipid metabolism by regulating the NRF2
            signaling pathway
  JOURNAL   J Mol Cell Biol 13 (7), 527-539 (2021)
   PUBMED   34048566
  REMARK    GeneRIF: PSA controls hepatic lipid metabolism by regulating the
            NRF2 signaling pathway.
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Zheng,X., Li,Q., Li,X., Zhang,Y., Wu,X., Wei,Q., Cao,S., Yang,M.,
            Lin,Z., Liao,Z., Qi,J., Lv,Q., Wang,L., Li,Y., Irwanto,A.,
            Cheng,C.Y., Chai,X., Khor,C.C., Heng,C.K., Koh,W.P., Yuan,J.M.,
            Bei,J., Zhang,F., Zhang,X., Zeng,Y., Shen,Y., Liu,J. and Gu,J.
  TITLE     Analysis of 47 Non-MHC Ankylosing Spondylitis Susceptibility Loci
            Regarding Associated Variants across Whites and Han Chinese
  JOURNAL   J Rheumatol 47 (5), 674-681 (2020)
   PUBMED   31523044
  REMARK    GeneRIF: Analysis of 47 Non-MHC Ankylosing Spondylitis
            Susceptibility Loci Regarding Associated Variants across Whites and
            Han Chinese.
REFERENCE   3  (bases 1 to 4309)
  AUTHORS   Perez,I., Blanco,L., Sanz,B., Errarte,P., Ariz,U., Beitia,M.,
            Fernandez,A., Loizate,A., Candenas,M.L., Pinto,F.M., Gil,J.,
            Lopez,J.I. and Larrinaga,G.
  TITLE     Altered Activity and Expression of Cytosolic Peptidases in
            Colorectal Cancer
  JOURNAL   Int J Med Sci 12 (6), 458-467 (2015)
   PUBMED   26078706
  REMARK    GeneRIF: cytosolic peptidases(puromycin-sensitive aminopeptidase
            (PSA), aminopeptidase B (APB) and pyroglutamyl-peptidase I (PGI) )
            may be involved in colorectal carcinogenesis
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4309)
  AUTHORS   Lemaitre,R.N., King,I.B., Kabagambe,E.K., Wu,J.H., McKnight,B.,
            Manichaikul,A., Guan,W., Sun,Q., Chasman,D.I., Foy,M., Wang,L.,
            Zhu,J., Siscovick,D.S., Tsai,M.Y., Arnett,D.K., Psaty,B.M.,
            Djousse,L., Chen,Y.D., Tang,W., Weng,L.C., Wu,H., Jensen,M.K.,
            Chu,A.Y., Jacobs,D.R. Jr., Rich,S.S., Mozaffarian,D., Steffen,L.,
            Rimm,E.B., Hu,F.B., Ridker,P.M., Fornage,M. and Friedlander,Y.
  TITLE     Genetic loci associated with circulating levels of very long-chain
            saturated fatty acids
  JOURNAL   J Lipid Res 56 (1), 176-184 (2015)
   PUBMED   25378659
REFERENCE   5  (bases 1 to 4309)
  AUTHORS   Lv,F. and Xue,Q.
  TITLE     [MiR-614 inhibited lung cancer cell invasion and proliferation via
            targeting PSA]
  JOURNAL   Zhongguo Fei Ai Za Zhi 17 (10), 715-721 (2014)
   PUBMED   25342037
  REMARK    GeneRIF: MiR-614 inhibited cell invasion and proliferationa
            targeting PSA in lung cancer cells, PGCL3
REFERENCE   6  (bases 1 to 4309)
  AUTHORS   Beninga,J., Rock,K.L. and Goldberg,A.L.
  TITLE     Interferon-gamma can stimulate post-proteasomal trimming of the N
            terminus of an antigenic peptide by inducing leucine aminopeptidase
  JOURNAL   J Biol Chem 273 (30), 18734-18742 (1998)
   PUBMED   9668046
REFERENCE   7  (bases 1 to 4309)
  AUTHORS   Tobler,A.R., Constam,D.B., Schmitt-Graff,A., Malipiero,U.,
            Schlapbach,R. and Fontana,A.
  TITLE     Cloning of the human puromycin-sensitive aminopeptidase and
            evidence for expression in neurons
  JOURNAL   J Neurochem 68 (3), 889-897 (1997)
   PUBMED   9048733
REFERENCE   8  (bases 1 to 4309)
  AUTHORS   Constam,D.B., Tobler,A.R., Rensing-Ehl,A., Kemler,I., Hersh,L.B.
            and Fontana,A.
  TITLE     Puromycin-sensitive aminopeptidase. Sequence analysis, expression,
            and functional characterization
  JOURNAL   J Biol Chem 270 (45), 26931-26939 (1995)
   PUBMED   7592939
REFERENCE   9  (bases 1 to 4309)
  AUTHORS   Cussenot,O., Berthon,P., Cochand-Priollet,B., Maitland,N.J. and Le
            Duc,A.
  TITLE     Immunocytochemical comparison of cultured normal epithelial
            prostatic cells with prostatic tissue sections
  JOURNAL   Exp Cell Res 214 (1), 83-92 (1994)
   PUBMED   8082751
REFERENCE   10 (bases 1 to 4309)
  AUTHORS   McLellan,S., Dyer,S.H., Rodriguez,G. and Hersh,L.B.
  TITLE     Studies on the tissue distribution of the puromycin-sensitive
            enkephalin-degrading aminopeptidases
  JOURNAL   J Neurochem 51 (5), 1552-1559 (1988)
   PUBMED   3171591
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB476543.3, BC065294.1,
            AJ132583.1 and BM723238.1.
            
            On Nov 22, 2018 this sequence version replaced NM_006310.3.
            
            Summary: This gene encodes the puromycin-sensitive aminopeptidase,
            a zinc metallopeptidase which hydrolyzes amino acids from the
            N-terminus of its substrate. The protein has been localized to both
            the cytoplasm and to cellular membranes. This enzyme degrades
            enkaphalins in the brain, and studies in mouse suggest that it is
            involved in proteolytic events regulating the cell cycle. [provided
            by RefSeq, Jul 2008].
            
            CCDS Note: Experimental evidence (N-terminal protein sequencing;
            PubMed ID 10978616) shows a peptide starting at Pro-46, suggesting
            that the downstream ATG (coding for Met-45) is used.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.63608.1,
                                           SRR1803612.124215.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000322157.9/ ENSP00000320324.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-693               DB476543.3         31-723
            694-3131            BC065294.1         413-2850
            3132-3156           AJ132583.1         2892-2916
            3157-3590           AJ132583.1         2918-3351
            3591-4054           AJ132583.1         3353-3816
            4055-4286           AJ132583.1         3820-4051
            4287-4309           BM723238.1         556-578
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q21.32"
     gene            1..4309
                     /gene="NPEPPS"
                     /gene_synonym="AAP-S; MP100; PSA"
                     /note="aminopeptidase puromycin sensitive"
                     /db_xref="GeneID:9520"
                     /db_xref="HGNC:HGNC:7900"
                     /db_xref="MIM:606793"
     exon            1..448
                     /gene="NPEPPS"
                     /gene_synonym="AAP-S; MP100; PSA"
                     /inference="alignment:Splign:2.1.0"
     CDS             194..2953
                     /gene="NPEPPS"
                     /gene_synonym="AAP-S; MP100; PSA"
                     /EC_number="3.4.11.14"
                     /note="isoform 1 is encoded by transcript variant 1;
                     puromycin-sensitive aminopeptidase; metalloproteinase
                     MP100; cytosol alanyl aminopeptidase"
                     /codon_start=1
                     /product="puromycin-sensitive aminopeptidase isoform 1"
                     /protein_id="NP_006301.3"
                     /db_xref="CCDS:CCDS45721.1"
                     /db_xref="GeneID:9520"
                     /db_xref="HGNC:HGNC:7900"
                     /db_xref="MIM:606793"
                     /translation="MWLAAAAPSLARRLLFLGPPPPPLLLLVFSRSSRRRLHSLGLAA
                     MPEKRPFERLPADVSPINYSLCLKPDLLDFTFEGKLEAAAQVRQATNQIVMNCADIDI
                     ITASYAPEGDEEIHATGFNYQNEDEKVTLSFPSTLQTGTGTLKIDFVGELNDKMKGFY
                     RSKYTTPSGEVRYAAVTQFEATDARRAFPCWDEPAIKATFDISLVVPKDRVALSNMNV
                     IDRKPYPDDENLVEVKFARTPVMSTYLVAFVVGEYDFVETRSKDGVCVRVYTPVGKAE
                     QGKFALEVAAKTLPFYKDYFNVPYPLPKIDLIAIADFAAGAMENWGLVTYRETALLID
                     PKNSCSSSRQWVALVVGHELAHQWFGNLVTMEWWTHLWLNEGFASWIEYLCVDHCFPE
                     YDIWTQFVSADYTRAQELDALDNSHPIEVSVGHPSEVDEIFDAISYSKGASVIRMLHD
                     YIGDKDFKKGMNMYLTKFQQKNAATEDLWESLENASGKPIAAVMNTWTKQMGFPLIYV
                     EAEQVEDDRLLRLSQKKFCAGGSYVGEDCPQWMVPITISTSEDPNQAKLKILMDKPEM
                     NVVLKNVKPDQWVKLNLGTVGFYRTQYSSAMLESLLPGIRDLSLPPVDRLGLQNDLFS
                     LARAGIISTVEVLKVMEAFVNEPNYTVWSDLSCNLGILSTLLSHTDFYEEIQEFVKDV
                     FSPIGERLGWDPKPGEGHLDALLRGLVLGKLGKAGHKATLEEARRRFKDHVEGKQILS
                     ADLRSPVYLTVLKHGDGTTLDIMLKLHKQADMQEEKNRIERVLGATLLPDLIQKVLTF
                     ALSEEVRPQDTVSVIGGVAGGSKHGRKAAWKFIKDNWEELYNRYQGGFLISRLIKLSV
                     EGFAVDKMAGEVKAFFESHPAPSAERTIQQCCENILLNAAWLKRDAESIHQYLLQRKA
                     SPPTV"
     exon            449..533
                     /gene="NPEPPS"
                     /gene_synonym="AAP-S; MP100; PSA"
                     /inference="alignment:Splign:2.1.0"
     exon            534..611
                     /gene="NPEPPS"
                     /gene_synonym="AAP-S; MP100; PSA"
                     /inference="alignment:Splign:2.1.0"
     exon            612..733
                     /gene="NPEPPS"
                     /gene_synonym="AAP-S; MP100; PSA"
                     /inference="alignment:Splign:2.1.0"
     exon            734..841
                     /gene="NPEPPS"
                     /gene_synonym="AAP-S; MP100; PSA"
                     /inference="alignment:Splign:2.1.0"
     exon            842..1042
                     /gene="NPEPPS"
                     /gene_synonym="AAP-S; MP100; PSA"
                     /inference="alignment:Splign:2.1.0"
     exon            1043..1139
                     /gene="NPEPPS"
                     /gene_synonym="AAP-S; MP100; PSA"
                     /inference="alignment:Splign:2.1.0"
     exon            1140..1173
                     /gene="NPEPPS"
                     /gene_synonym="AAP-S; MP100; PSA"
                     /inference="alignment:Splign:2.1.0"
     exon            1174..1288
                     /gene="NPEPPS"
                     /gene_synonym="AAP-S; MP100; PSA"
                     /inference="alignment:Splign:2.1.0"
     exon            1289..1453
                     /gene="NPEPPS"
                     /gene_synonym="AAP-S; MP100; PSA"
                     /inference="alignment:Splign:2.1.0"
     exon            1454..1558
                     /gene="NPEPPS"
                     /gene_synonym="AAP-S; MP100; PSA"
                     /inference="alignment:Splign:2.1.0"
     exon            1559..1619
                     /gene="NPEPPS"
                     /gene_synonym="AAP-S; MP100; PSA"
                     /inference="alignment:Splign:2.1.0"
     exon            1620..1729
                     /gene="NPEPPS"
                     /gene_synonym="AAP-S; MP100; PSA"
                     /inference="alignment:Splign:2.1.0"
     exon            1730..1793
                     /gene="NPEPPS"
                     /gene_synonym="AAP-S; MP100; PSA"
                     /inference="alignment:Splign:2.1.0"
     exon            1794..1933
                     /gene="NPEPPS"
                     /gene_synonym="AAP-S; MP100; PSA"
                     /inference="alignment:Splign:2.1.0"
     exon            1934..2068
                     /gene="NPEPPS"
                     /gene_synonym="AAP-S; MP100; PSA"
                     /inference="alignment:Splign:2.1.0"
     exon            2069..2288
                     /gene="NPEPPS"
                     /gene_synonym="AAP-S; MP100; PSA"
                     /inference="alignment:Splign:2.1.0"
     exon            2289..2431
                     /gene="NPEPPS"
                     /gene_synonym="AAP-S; MP100; PSA"
                     /inference="alignment:Splign:2.1.0"
     exon            2432..2488
                     /gene="NPEPPS"
                     /gene_synonym="AAP-S; MP100; PSA"
                     /inference="alignment:Splign:2.1.0"
     exon            2489..2596
                     /gene="NPEPPS"
                     /gene_synonym="AAP-S; MP100; PSA"
                     /inference="alignment:Splign:2.1.0"
     exon            2597..2752
                     /gene="NPEPPS"
                     /gene_synonym="AAP-S; MP100; PSA"
                     /inference="alignment:Splign:2.1.0"
     exon            2753..2800
                     /gene="NPEPPS"
                     /gene_synonym="AAP-S; MP100; PSA"
                     /inference="alignment:Splign:2.1.0"
     exon            2801..4309
                     /gene="NPEPPS"
                     /gene_synonym="AAP-S; MP100; PSA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3194..3199
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NPEPPS"
                     /gene_synonym="AAP-S; MP100; PSA"
                     /note="hexamer: AATAGA"
     polyA_site      3218
                     /gene="NPEPPS"
                     /gene_synonym="AAP-S; MP100; PSA"
     regulatory      4285..4290
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NPEPPS"
                     /gene_synonym="AAP-S; MP100; PSA"
                     /note="hexamer: AATAAA"
     polyA_site      4309
                     /gene="NPEPPS"
                     /gene_synonym="AAP-S; MP100; PSA"
                     /note="major polyA site"
ORIGIN      
        1 acttccccct ctccctccct ccttgcgggc cctcctcccc ttccctcccc tccgccccct
       61 tccccgtagg cagcccgccc gccagtccgc ccgcaccgcc tccttcccag cccctagcgc
      121 tccggctggg tctctccccc gccccccagg ctcccccggt cgctctcctc cggcggtcgc
      181 ccgcgctcgg tggatgtggc tggcagctgc cgccccctcc ctcgctcgcc gcctgctctt
      241 cctcggccct ccgcctcctc ccctcctcct tctcgtcttc agccgctcct ctcgccgccg
      301 cctccacagc ctgggcctcg ccgcgatgcc ggagaagagg cccttcgagc ggctgcctgc
      361 cgatgtctcc cccatcaact acagcctttg cctcaagccc gacttgctgg acttcacctt
      421 cgagggcaag ctggaggccg ccgcccaggt gaggcaggcg actaatcaga ttgtgatgaa
      481 ttgtgctgat attgatatta ttacagcttc atatgcacca gaaggagatg aagaaataca
      541 tgctacagga tttaactatc agaatgaaga tgaaaaagtc accttgtctt tccctagtac
      601 tctgcaaaca ggtacgggaa ccttaaagat agattttgtt ggagagctga atgacaaaat
      661 gaaaggtttc tatagaagta aatatactac cccttctgga gaggtgcgct atgctgctgt
      721 aacacagttt gaggctactg atgcccgaag ggcttttcct tgctgggatg agcctgctat
      781 caaagcaact tttgatatct cattggttgt tcctaaagac agagtagctt tatcaaacat
      841 gaatgtaatt gaccggaaac cataccctga tgatgaaaat ttagtggaag tgaagtttgc
      901 ccgcacacct gttatgtcta catatctggt ggcatttgtt gtgggtgaat atgactttgt
      961 agaaacaagg tcaaaagatg gtgtgtgtgt ccgtgtttac actcctgttg gcaaagcaga
     1021 gcaaggaaaa tttgcgttag aggttgctgc taaaaccttg cctttttata aggactactt
     1081 caatgttcct tatcctctac ctaaaattga tctcattgct attgcagact ttgcagctgg
     1141 tgccatggag aactggggcc ttgttactta tagggagact gcattgctta ttgatccaaa
     1201 aaattcctgt tcttcatccc gccagtgggt tgctctggtt gtgggacatg aactcgccca
     1261 tcaatggttt ggaaatcttg ttactatgga atggtggact catctttggt taaatgaagg
     1321 ttttgcatcc tggattgaat atctgtgtgt agaccactgc ttcccagagt atgatatttg
     1381 gactcagttt gtttctgctg attacacccg tgcccaggag cttgacgcct tagataacag
     1441 ccatcctatt gaagtcagtg tgggccatcc atctgaggtt gatgagatat ttgatgctat
     1501 atcatatagc aaaggtgcat ctgtcatccg aatgctgcat gactacattg gggataagga
     1561 ctttaagaaa ggaatgaaca tgtatttaac caagttccaa caaaagaatg ctgccacaga
     1621 ggatctctgg gaaagtttag aaaatgctag tggtaaacct atagcagctg tgatgaatac
     1681 ctggaccaaa caaatgggat ttcccctcat ttatgtggaa gctgaacagg tagaagatga
     1741 cagattattg aggttgtccc aaaagaagtt ctgtgctggt gggtcatatg ttggtgaaga
     1801 ttgtccccag tggatggtcc ctatcacaat ctctactagt gaagacccca accaggccaa
     1861 actaaaaatt ctaatggaca agccagagat gaatgtggtt ttgaaaaatg tcaaaccaga
     1921 ccaatgggtg aagttaaact taggaacagt tgggttttat cggacccagt acagctctgc
     1981 catgctggaa agtttattac caggcattcg tgacctttct ctgccccctg tggatcgact
     2041 tggattacag aatgacctct tctccttggc tcgagctgga atcattagca ctgtagaggt
     2101 tctaaaagtc atggaggctt ttgtgaatga gcccaattat actgtatgga gcgacctgag
     2161 ctgtaacctg gggattctct caactctctt gtcccacaca gacttctatg aggaaatcca
     2221 ggagtttgtg aaagatgtct tttcacctat aggggagaga ctgggctggg accccaaacc
     2281 tggagaaggt catctcgatg cactcctgag gggcttggtt ctgggaaaac taggaaaagc
     2341 aggacataag gcaacgttag aagaagcccg tcgtcggttt aaggaccacg tggaaggaaa
     2401 acagattctc tccgctgatc tgaggagtcc tgtctatctg actgttttga agcatggtga
     2461 tggcactact ttagatatta tgttaaaact tcataaacaa gcagatatgc aagaagagaa
     2521 aaaccgaatc gaaagagtcc ttggcgctac tcttttgcct gacctgattc aaaaagtcct
     2581 cacgtttgca ctttcagaag aggtacgtcc acaggacact gtatcggtaa ttggtggagt
     2641 agctggaggc agcaagcatg gtaggaaagc tgcttggaaa ttcataaagg acaactggga
     2701 agaactttat aaccgatacc agggaggatt cttaatatcc agactaataa agctatcagt
     2761 tgagggattt gcagttgata aaatggctgg agaggttaag gctttcttcg agagtcaccc
     2821 agctccttca gctgagcgta ccatccagca gtgttgtgaa aatattctgc tgaatgctgc
     2881 ctggctaaag cgagatgctg agagcatcca ccagtacctc cttcagcgga aggcctcacc
     2941 acccacagtg tgaatcctga ggtgccgcca ttggcggttc tgctgcttcg ctgcagggat
     3001 aaggtggagc taccgaacag ctgattcata tgccaagaat ttggagtctt ctttcaaacc
     3061 agtgggggtt ggacaatgaa tgtagttaac tggttcctgc tcacactcca gaattaaatt
     3121 ctattgaaaa aggaaaatca gcaattcagc aaaaaataaa taaaaaataa aaatgtaaat
     3181 atgatagtaa taaaatagag cataacgaaa ctgtgaaact ttctgaagcc ttgtcagtgg
     3241 ttaaaagtat ttaacactct actgttaatg acagatgttc tgtttttata acctaccaaa
     3301 aggaaactag aggcttcttg gtgaagagca tttttgtgaa gtgggttctg caaggagcct
     3361 ataaagccaa gggtggtgtc catttctggg aatggttaaa cacaaaaggc tgatagctgg
     3421 tatcacatag ttggagtcag tgcataattc caagtggctt tttttttttt tggcacgggg
     3481 actgatcagg aagatatatt cctgcataac tcaatctgaa ccaaggattg tagtttagtt
     3541 ttcctccttg ccttcccttc tgtgtgaccg accccttggc caaaaaaaaa caaaaagcaa
     3601 aaaacaaaaa cctaccctgt tctggttttt ttcctccctt tagttccacc cccaaccccc
     3661 attccctggt gtccttctta gagatgaaga aataataagg aaacatcttt catagccaca
     3721 ttaaataaga gaaactgata tacattattt ttttcttttt aaagatgact tataagaacc
     3781 ctgaaattta tataggtgag acaatagaaa taaaaagatc ttcagccagg cctttctgaa
     3841 ggagttattc tgctaaaaat ggtcttagtt gtctgaaaag ccagctcttg aacctcttca
     3901 caacagtatc aacactggct tctcccggtt cattttatgc gtgcgagaag tcagtggtaa
     3961 ctgctgcagg gcttaataca ttagtggtaa ctggtttaaa aaacaaagac tgtaagcctg
     4021 tgtgtgccac tgtttgcttc aacagtatat cctaataagc ctcacctatt taatccaatg
     4081 agttttaaat ctaaatctca ttcccttctt ctttccctac cttttttttc tttttttctt
     4141 aaaaaaatat tttgtgttat taacagaaat tcatatttgg tgtggcttaa cggtatttca
     4201 gaaggtcatc agattgtgag actgcttcct tgaaacattt ttgtgctatt gttttaaaaa
     4261 aataattaaa aaacagttgg cgttaataaa aatgtcaatg tgaaactga
//
