LOCUS       NP_006301                919 aa            linear   PRI 28-APR-2025
DEFINITION  puromycin-sensitive aminopeptidase isoform 1 [Homo sapiens].
ACCESSION   NP_006301 XP_001128588
VERSION     NP_006301.3
DBSOURCE    REFSEQ: accession NM_006310.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 919)
  AUTHORS   Huang,B., Xiong,X., Zhang,L., Liu,X., Wang,Y., Gong,X., Sang,Q.,
            Lu,Y., Qu,H., Zheng,H. and Zheng,Y.
  TITLE     PSA controls hepatic lipid metabolism by regulating the NRF2
            signaling pathway
  JOURNAL   J Mol Cell Biol 13 (7), 527-539 (2021)
   PUBMED   34048566
  REMARK    GeneRIF: PSA controls hepatic lipid metabolism by regulating the
            NRF2 signaling pathway.
REFERENCE   2  (residues 1 to 919)
  AUTHORS   Zheng,X., Li,Q., Li,X., Zhang,Y., Wu,X., Wei,Q., Cao,S., Yang,M.,
            Lin,Z., Liao,Z., Qi,J., Lv,Q., Wang,L., Li,Y., Irwanto,A.,
            Cheng,C.Y., Chai,X., Khor,C.C., Heng,C.K., Koh,W.P., Yuan,J.M.,
            Bei,J., Zhang,F., Zhang,X., Zeng,Y., Shen,Y., Liu,J. and Gu,J.
  TITLE     Analysis of 47 Non-MHC Ankylosing Spondylitis Susceptibility Loci
            Regarding Associated Variants across Whites and Han Chinese
  JOURNAL   J Rheumatol 47 (5), 674-681 (2020)
   PUBMED   31523044
  REMARK    GeneRIF: Analysis of 47 Non-MHC Ankylosing Spondylitis
            Susceptibility Loci Regarding Associated Variants across Whites and
            Han Chinese.
REFERENCE   3  (residues 1 to 919)
  AUTHORS   Perez,I., Blanco,L., Sanz,B., Errarte,P., Ariz,U., Beitia,M.,
            Fernandez,A., Loizate,A., Candenas,M.L., Pinto,F.M., Gil,J.,
            Lopez,J.I. and Larrinaga,G.
  TITLE     Altered Activity and Expression of Cytosolic Peptidases in
            Colorectal Cancer
  JOURNAL   Int J Med Sci 12 (6), 458-467 (2015)
   PUBMED   26078706
  REMARK    GeneRIF: cytosolic peptidases(puromycin-sensitive aminopeptidase
            (PSA), aminopeptidase B (APB) and pyroglutamyl-peptidase I (PGI) )
            may be involved in colorectal carcinogenesis
            Publication Status: Online-Only
REFERENCE   4  (residues 1 to 919)
  AUTHORS   Lemaitre,R.N., King,I.B., Kabagambe,E.K., Wu,J.H., McKnight,B.,
            Manichaikul,A., Guan,W., Sun,Q., Chasman,D.I., Foy,M., Wang,L.,
            Zhu,J., Siscovick,D.S., Tsai,M.Y., Arnett,D.K., Psaty,B.M.,
            Djousse,L., Chen,Y.D., Tang,W., Weng,L.C., Wu,H., Jensen,M.K.,
            Chu,A.Y., Jacobs,D.R. Jr., Rich,S.S., Mozaffarian,D., Steffen,L.,
            Rimm,E.B., Hu,F.B., Ridker,P.M., Fornage,M. and Friedlander,Y.
  TITLE     Genetic loci associated with circulating levels of very long-chain
            saturated fatty acids
  JOURNAL   J Lipid Res 56 (1), 176-184 (2015)
   PUBMED   25378659
REFERENCE   5  (residues 1 to 919)
  AUTHORS   Lv,F. and Xue,Q.
  TITLE     [MiR-614 inhibited lung cancer cell invasion and proliferation via
            targeting PSA]
  JOURNAL   Zhongguo Fei Ai Za Zhi 17 (10), 715-721 (2014)
   PUBMED   25342037
  REMARK    GeneRIF: MiR-614 inhibited cell invasion and proliferationa
            targeting PSA in lung cancer cells, PGCL3
REFERENCE   6  (residues 1 to 919)
  AUTHORS   Beninga,J., Rock,K.L. and Goldberg,A.L.
  TITLE     Interferon-gamma can stimulate post-proteasomal trimming of the N
            terminus of an antigenic peptide by inducing leucine aminopeptidase
  JOURNAL   J Biol Chem 273 (30), 18734-18742 (1998)
   PUBMED   9668046
REFERENCE   7  (residues 1 to 919)
  AUTHORS   Tobler,A.R., Constam,D.B., Schmitt-Graff,A., Malipiero,U.,
            Schlapbach,R. and Fontana,A.
  TITLE     Cloning of the human puromycin-sensitive aminopeptidase and
            evidence for expression in neurons
  JOURNAL   J Neurochem 68 (3), 889-897 (1997)
   PUBMED   9048733
REFERENCE   8  (residues 1 to 919)
  AUTHORS   Constam,D.B., Tobler,A.R., Rensing-Ehl,A., Kemler,I., Hersh,L.B.
            and Fontana,A.
  TITLE     Puromycin-sensitive aminopeptidase. Sequence analysis, expression,
            and functional characterization
  JOURNAL   J Biol Chem 270 (45), 26931-26939 (1995)
   PUBMED   7592939
REFERENCE   9  (residues 1 to 919)
  AUTHORS   Cussenot,O., Berthon,P., Cochand-Priollet,B., Maitland,N.J. and Le
            Duc,A.
  TITLE     Immunocytochemical comparison of cultured normal epithelial
            prostatic cells with prostatic tissue sections
  JOURNAL   Exp Cell Res 214 (1), 83-92 (1994)
   PUBMED   8082751
REFERENCE   10 (residues 1 to 919)
  AUTHORS   McLellan,S., Dyer,S.H., Rodriguez,G. and Hersh,L.B.
  TITLE     Studies on the tissue distribution of the puromycin-sensitive
            enkephalin-degrading aminopeptidases
  JOURNAL   J Neurochem 51 (5), 1552-1559 (1988)
   PUBMED   3171591
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB476543.3, BC065294.1,
            AJ132583.1 and BM723238.1.
            
            On Oct 31, 2007 this sequence version replaced NP_006301.2.
            
            Summary: This gene encodes the puromycin-sensitive aminopeptidase,
            a zinc metallopeptidase which hydrolyzes amino acids from the
            N-terminus of its substrate. The protein has been localized to both
            the cytoplasm and to cellular membranes. This enzyme degrades
            enkaphalins in the brain, and studies in mouse suggest that it is
            involved in proteolytic events regulating the cell cycle. [provided
            by RefSeq, Jul 2008].
            
            CCDS Note: Experimental evidence (N-terminal protein sequencing;
            PubMed ID 10978616) shows a peptide starting at Pro-46, suggesting
            that the downstream ATG (coding for Met-45) is used.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660805.63608.1,
                                           SRR1803612.124215.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000322157.9/ ENSP00000320324.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q21.32"
     Protein         1..919
                     /product="puromycin-sensitive aminopeptidase isoform 1"
                     /EC_number="3.4.11.14"
                     /note="puromycin-sensitive aminopeptidase;
                     metalloproteinase MP100; cytosol alanyl aminopeptidase"
                     /calculated_mol_wt=103146
     Region          61..499
                     /region_name="M1_APN-Q_like"
                     /note="Peptidase M1 aminopeptidase N catalytic domain
                     family which includes aminopeptidase N (APN),
                     aminopeptidase Q (APQ), tricorn interacting factor F3, and
                     endoplasmic reticulum aminopeptidase 1 (ERAP1); cd09601"
                     /db_xref="CDD:341064"
     Site            order(178,180,316..319,352..353,356,375,379,433,438)
                     /site_type="active"
                     /db_xref="CDD:341064"
     Site            438
                     /site_type="other"
                     /note="Transition state stabilizer. /evidence=ECO:0000250;
                     propagated from UniProtKB/Swiss-Prot (P55786.2)"
     Region          578..891
                     /region_name="ERAP1_C"
                     /note="ERAP1-like C-terminal domain; pfam11838"
                     /db_xref="CDD:463368"
     Region          726..730
                     /region_name="Nuclear localization signal.
                     /evidence=ECO:0000255"
                     /note="propagated from UniProtKB/Swiss-Prot (P55786.2)"
     CDS             1..919
                     /gene="NPEPPS"
                     /gene_synonym="AAP-S; MP100; PSA"
                     /coded_by="NM_006310.4:194..2953"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /db_xref="CCDS:CCDS45721.1"
                     /db_xref="GeneID:9520"
                     /db_xref="HGNC:HGNC:7900"
                     /db_xref="MIM:606793"
ORIGIN      
        1 mwlaaaapsl arrllflgpp pppllllvfs rssrrrlhsl glaampekrp ferlpadvsp
       61 inyslclkpd lldftfegkl eaaaqvrqat nqivmncadi diitasyape gdeeihatgf
      121 nyqnedekvt lsfpstlqtg tgtlkidfvg elndkmkgfy rskyttpsge vryaavtqfe
      181 atdarrafpc wdepaikatf dislvvpkdr valsnmnvid rkpypddenl vevkfartpv
      241 mstylvafvv geydfvetrs kdgvcvrvyt pvgkaeqgkf alevaaktlp fykdyfnvpy
      301 plpkidliai adfaagamen wglvtyreta llidpknscs ssrqwvalvv ghelahqwfg
      361 nlvtmewwth lwlnegfasw ieylcvdhcf peydiwtqfv sadytraqel daldnshpie
      421 vsvghpsevd eifdaisysk gasvirmlhd yigdkdfkkg mnmyltkfqq knaatedlwe
      481 slenasgkpi aavmntwtkq mgfpliyvea eqveddrllr lsqkkfcagg syvgedcpqw
      541 mvpitistse dpnqaklkil mdkpemnvvl knvkpdqwvk lnlgtvgfyr tqyssamles
      601 llpgirdlsl ppvdrlglqn dlfslaragi istvevlkvm eafvnepnyt vwsdlscnlg
      661 ilstllshtd fyeeiqefvk dvfspigerl gwdpkpgegh ldallrglvl gklgkaghka
      721 tleearrrfk dhvegkqils adlrspvylt vlkhgdgttl dimlklhkqa dmqeeknrie
      781 rvlgatllpd liqkvltfal seevrpqdtv sviggvaggs khgrkaawkf ikdnweelyn
      841 ryqggflisr liklsvegfa vdkmagevka ffeshpapsa ertiqqccen illnaawlkr
      901 daesihqyll qrkaspptv
//
