LOCUS       NM_001101648            4982 bp    mRNA    linear   PRI 24-OCT-2024
DEFINITION  Homo sapiens NPC1 like intracellular cholesterol transporter 1
            (NPC1L1), transcript variant 2, mRNA.
ACCESSION   NM_001101648
VERSION     NM_001101648.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4982)
  AUTHORS   Cho,D., Huang,X., Han,Y. and Kim,M.
  TITLE     NPC1L1 rs217434 A > G as a Novel Single Nucleotide Polymorphism
            Related to Dyslipidemia in a Korean Population
  JOURNAL   Biochem Genet 62 (5), 4103-4119 (2024)
   PUBMED   38280151
  REMARK    GeneRIF: NPC1L1 rs217434 A > G as a Novel Single Nucleotide
            Polymorphism Related to Dyslipidemia in a Korean Population.
REFERENCE   2  (bases 1 to 4982)
  AUTHORS   Butt,E., Gunder,T., Sturzebecher,P., Kowalski,I., Schneider,P.,
            Buschmann,N., Schafer,S., Bender,A., Hermanns,H.M. and Zernecke,A.
  TITLE     Cholesterol uptake in the intestine is regulated by the
            LASP1-AKT-NPC1L1 signaling pathway
  JOURNAL   Am J Physiol Gastrointest Liver Physiol 327 (1), G25-G35 (2024)
   PUBMED   38713618
  REMARK    GeneRIF: Cholesterol uptake in the intestine is regulated by the
            LASP1-AKT-NPC1L1 signaling pathway.
REFERENCE   3  (bases 1 to 4982)
  AUTHORS   Li,Y., Li,J., Tang,X., Xu,J., Liu,R., Jiang,L., Tian,J., Zhang,Y.,
            Wang,D., Sun,K., Xu,B., Zhao,W., Hui,R., Gao,R., Song,L., Yuan,J.
            and Zhao,X.
  TITLE     Association of NPC1L1 and HMGCR gene polymorphisms with coronary
            artery calcification in patients with premature triple-vessel
            coronary disease
  JOURNAL   BMC Med Genomics 17 (1), 22 (2024)
   PUBMED   38233830
  REMARK    GeneRIF: Association of NPC1L1 and HMGCR gene polymorphisms with
            coronary artery calcification in patients with premature
            triple-vessel coronary disease.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4982)
  AUTHORS   Li,J., Tang,X., Xu,J., Liu,R., Jiang,L., Xu,L., Tian,J., Feng,X.,
            Wu,Y., Zhang,Y., Wang,D., Sun,K., Xu,B., Zhao,W., Hui,R., Gao,R.,
            Song,L., Yuan,J. and Zhao,X.
  TITLE     HMGCR gene polymorphism is associated with residual cholesterol
            risk in premature triple-vessel disease patients treated with
            moderate-intensity statins
  JOURNAL   BMC Cardiovasc Disord 23 (1), 317 (2023)
   PUBMED   37355634
  REMARK    GeneRIF: HMGCR gene polymorphism is associated with residual
            cholesterol risk in premature triple-vessel disease patients
            treated with moderate-intensity statins.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4982)
  AUTHORS   Zhang,G., Li,X. and Huang,X.
  TITLE     m6A-related bioinformatics analysis and functional characterization
            reveals that METTL3-mediated NPC1L1 mRNA hypermethylation
            facilitates progression of atherosclerosis via inactivation of the
            MAPK pathway
  JOURNAL   Inflamm Res 72 (3), 429-442 (2023)
   PUBMED   36583755
  REMARK    GeneRIF: m6A-related bioinformatics analysis and functional
            characterization reveals that METTL3-mediated NPC1L1 mRNA
            hypermethylation facilitates progression of atherosclerosis via
            inactivation of the MAPK pathway.
REFERENCE   6  (bases 1 to 4982)
  AUTHORS   Davies,J.P., Scott,C., Oishi,K., Liapis,A. and Ioannou,Y.A.
  TITLE     Inactivation of NPC1L1 causes multiple lipid transport defects and
            protects against diet-induced hypercholesterolemia
  JOURNAL   J Biol Chem 280 (13), 12710-12720 (2005)
   PUBMED   15671032
  REMARK    GeneRIF: NPC1L1 has a role in lipid transport and in diet-induced
            hypercholesterolemia
REFERENCE   7  (bases 1 to 4982)
  AUTHORS   van der Veen,J.N., Kruit,J.K., Havinga,R., Baller,J.F., Chimini,G.,
            Lestavel,S., Staels,B., Groot,P.H., Groen,A.K. and Kuipers,F.
  TITLE     Reduced cholesterol absorption upon PPARdelta activation coincides
            with decreased intestinal expression of NPC1L1
  JOURNAL   J Lipid Res 46 (3), 526-534 (2005)
   PUBMED   15604518
REFERENCE   8  (bases 1 to 4982)
  AUTHORS   Wang,J., Williams,C.M. and Hegele,R.A.
  TITLE     Compound heterozygosity for two non-synonymous polymorphisms in
            NPC1L1 in a non-responder to ezetimibe
  JOURNAL   Clin Genet 67 (2), 175-177 (2005)
   PUBMED   15679830
REFERENCE   9  (bases 1 to 4982)
  AUTHORS   Altmann,S.W., Davis,H.R. Jr., Zhu,L.J., Yao,X., Hoos,L.M.,
            Tetzloff,G., Iyer,S.P., Maguire,M., Golovko,A., Zeng,M., Wang,L.,
            Murgolo,N. and Graziano,M.P.
  TITLE     Niemann-Pick C1 Like 1 protein is critical for intestinal
            cholesterol absorption
  JOURNAL   Science 303 (5661), 1201-1204 (2004)
   PUBMED   14976318
  REMARK    GeneRIF: expression is enriched in the small intestine and is in
            the brush border membrane of enterocytes
REFERENCE   10 (bases 1 to 4982)
  AUTHORS   Davies,J.P., Levy,B. and Ioannou,Y.A.
  TITLE     Evidence for a Niemann-pick C (NPC) gene family: identification and
            characterization of NPC1L1
  JOURNAL   Genomics 65 (2), 137-145 (2000)
   PUBMED   10783261
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC004938.3, AF192522.1 and
            AY515256.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_001101648.1.
            
            Summary: The protein encoded by this gene is a multi-pass membrane
            protein. It contains a conserved N-terminal Niemann-Pick C1 (NPC1)
            domain and a putative sterol-sensing domain (SSD) which includes a
            YQRL motif functioning as a plasma membrane to trans-Golgi network
            transport signal in other proteins. This protein takes up free
            cholesterol into cells through vesicular endocytosis and plays a
            critical role in the absorption of intestinal cholesterol. It also
            has the ability to transport alpha-tocopherol (vitamin E). The drug
            ezetimibe targets this protein and inhibits the absorption of
            intestinal cholesterol and alpha-tocopherol. In addition, this
            protein may play a critical role in regulating lipid metabolism.
            Polymorphic variations in this gene are associated with plasma
            total cholesterol and low-density lipoprotein cholesterol (LDL-C)
            levels and coronary heart disease (CHD) risk. Alternatively spliced
            transcript variants encoding different isoforms have been found for
            this gene. [provided by RefSeq, Oct 2009].
            
            Transcript Variant: This variant (2) lacks an alternate in-frame
            exon in the 3' coding region, compared to variant 1, resulting in a
            shorter protein (isoform 2), compared to isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038195.1791879.1, BC117178.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1966682,
                                           SAMEA1968189 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000381160.8/ ENSP00000370552.3
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-15                AC004938.3         47874-47888
            16-71               AF192522.1         1-56
            72-4070             AY515256.1         1-3999
            4071-4982           AF192522.1         4137-5048
FEATURES             Location/Qualifiers
     source          1..4982
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="7"
                     /map="7p13"
     gene            1..4982
                     /gene="NPC1L1"
                     /gene_synonym="LDLCQ7; NPC11L1; SLC65A2"
                     /note="NPC1 like intracellular cholesterol transporter 1"
                     /db_xref="GeneID:29881"
                     /db_xref="HGNC:HGNC:7898"
                     /db_xref="MIM:608010"
     exon            1..125
                     /gene="NPC1L1"
                     /gene_synonym="LDLCQ7; NPC11L1; SLC65A2"
                     /inference="alignment:Splign:2.1.0"
     CDS             72..4070
                     /gene="NPC1L1"
                     /gene_synonym="LDLCQ7; NPC11L1; SLC65A2"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 2; niemann-Pick C1-like protein 1; NPC1
                     (Niemann-Pick disease, type C1, gene)-like 1; NPC1 like 1"
                     /codon_start=1
                     /product="NPC1-like intracellular cholesterol transporter
                     1 isoform 2 precursor"
                     /protein_id="NP_001095118.1"
                     /db_xref="CCDS:CCDS43575.1"
                     /db_xref="GeneID:29881"
                     /db_xref="HGNC:HGNC:7898"
                     /db_xref="MIM:608010"
                     /translation="MAEAGLRGWLLWALLLRLAQSEPYTTIHQPGYCAFYDECGKNPE
                     LSGSLMTLSNVSCLSNTPARKITGDHLILLQKICPRLYTGPNTQACCSAKQLVSLEAS
                     LSITKALLTRCPACSDNFVNLHCHNTCSPNQSLFINVTRVAQLGAGQLPAVVAYEAFY
                     QHSFAEQSYDSCSRVRVPAAATLAVGTMCGVYGSALCNAQRWLNFQGDTGNGLAPLDI
                     TFHLLEPGQAVGSGIQPLNEGVARCNESQGDDVATCSCQDCAASCPAIARPQALDSTF
                     YLGQMPGSLVLIIILCSVFAVVTILLVGFRVAPARDKSKMVDPKKGTSLSDKLSFSTH
                     TLLGQFFQGWGTWVASWPLTILVLSVIPVVALAAGLVFTELTTDPVELWSAPNSQARS
                     EKAFHDQHFGPFFRTNQVILTAPNRSSYRYDSLLLGPKNFSGILDLDLLLELLELQER
                     LRHLQVWSPEAQRNISLQDICYAPLNPDNTSLYDCCINSLLQYFQNNRTLLLLTANQT
                     LMGQTSQVDWKDHFLYCANAPLTFKDGTALALSCMADYGAPVFPFLAIGGYKGKDYSE
                     AEALIMTFSLNNYPAGDPRLAQAKLWEEAFLEEMRAFQRRMAGMFQVTFMAERSLEDE
                     INRTTAEDLPIFATSYIVIFLYISLALGSYSSWSRVMVDSKATLGLGGVAVVLGAVMA
                     AMGFFSYLGIRSSLVILQVVPFLVLSVGADNIFIFVLEYQRLPRRPGEPREVHIGRAL
                     GRVAPSMLLCSLSEAICFFLGALTPMPAVRTFALTSGLAVILDFLLQMSAFVALLSLD
                     SKRQEASRLDVCCCVKPQELPPPGQGEGLLLGFFQKAYAPFLLHWITRGVVLLLFLAL
                     FGVSLYSMCHISVGLDQELALPKDSYLLDYFLFLNRYFEVGAPVYFVTTLGYNFSSEA
                     GMNAICSSAGCNNFSFTQKIQYATEFPEQSYLAIPASSWVDDFIDWLTPSSCCRLYIS
                     GPNKDKFCPSTVNSLNCLKNCMSITMGSVRPSVEQFHKYLPWFLNDRPNIKCPKGGLA
                     AYSTSVNLTSDGQVLASRFMAYHKPLKNSQDYTEALRAARELAANITADLRKVPGTDP
                     AFEVFPYTITNVFYEQYLTILPEGLFMLSLCLVPTFAVSCLLLGLDLRSGLLNLLSIV
                     MILVDTVGFMALWGISYNAVSLINLVSAVGMSVEFVSHITRSFAISTKPTWLERAKEA
                     TISMGSAVFAGVAMTNLPGILVLGLAKAQLIQIFFFRLNLLITLLGLLHGLVFLPVIL
                     SYVGPDVNPALALEQKRAEEAVAAVMVASCPNHPSRVSTADNIYVNHSFEGSIKGAGA
                     ISNFLPNNGRQF"
     exon            126..1651
                     /gene="NPC1L1"
                     /gene_synonym="LDLCQ7; NPC11L1; SLC65A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1652..1752
                     /gene="NPC1L1"
                     /gene_synonym="LDLCQ7; NPC11L1; SLC65A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1753..1925
                     /gene="NPC1L1"
                     /gene_synonym="LDLCQ7; NPC11L1; SLC65A2"
                     /inference="alignment:Splign:2.1.0"
     exon            1926..2054
                     /gene="NPC1L1"
                     /gene_synonym="LDLCQ7; NPC11L1; SLC65A2"
                     /inference="alignment:Splign:2.1.0"
     exon            2055..2237
                     /gene="NPC1L1"
                     /gene_synonym="LDLCQ7; NPC11L1; SLC65A2"
                     /inference="alignment:Splign:2.1.0"
     exon            2238..2352
                     /gene="NPC1L1"
                     /gene_synonym="LDLCQ7; NPC11L1; SLC65A2"
                     /inference="alignment:Splign:2.1.0"
     exon            2353..2480
                     /gene="NPC1L1"
                     /gene_synonym="LDLCQ7; NPC11L1; SLC65A2"
                     /inference="alignment:Splign:2.1.0"
     exon            2481..2618
                     /gene="NPC1L1"
                     /gene_synonym="LDLCQ7; NPC11L1; SLC65A2"
                     /inference="alignment:Splign:2.1.0"
     exon            2619..2708
                     /gene="NPC1L1"
                     /gene_synonym="LDLCQ7; NPC11L1; SLC65A2"
                     /inference="alignment:Splign:2.1.0"
     exon            2709..2899
                     /gene="NPC1L1"
                     /gene_synonym="LDLCQ7; NPC11L1; SLC65A2"
                     /inference="alignment:Splign:2.1.0"
     exon            2900..3024
                     /gene="NPC1L1"
                     /gene_synonym="LDLCQ7; NPC11L1; SLC65A2"
                     /inference="alignment:Splign:2.1.0"
     exon            3025..3151
                     /gene="NPC1L1"
                     /gene_synonym="LDLCQ7; NPC11L1; SLC65A2"
                     /inference="alignment:Splign:2.1.0"
     exon            3152..3207
                     /gene="NPC1L1"
                     /gene_synonym="LDLCQ7; NPC11L1; SLC65A2"
                     /inference="alignment:Splign:2.1.0"
     exon            3208..3358
                     /gene="NPC1L1"
                     /gene_synonym="LDLCQ7; NPC11L1; SLC65A2"
                     /inference="alignment:Splign:2.1.0"
     exon            3359..3590
                     /gene="NPC1L1"
                     /gene_synonym="LDLCQ7; NPC11L1; SLC65A2"
                     /inference="alignment:Splign:2.1.0"
     exon            3591..3704
                     /gene="NPC1L1"
                     /gene_synonym="LDLCQ7; NPC11L1; SLC65A2"
                     /inference="alignment:Splign:2.1.0"
     exon            3705..3867
                     /gene="NPC1L1"
                     /gene_synonym="LDLCQ7; NPC11L1; SLC65A2"
                     /inference="alignment:Splign:2.1.0"
     exon            3868..4982
                     /gene="NPC1L1"
                     /gene_synonym="LDLCQ7; NPC11L1; SLC65A2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4961..4966
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NPC1L1"
                     /gene_synonym="LDLCQ7; NPC11L1; SLC65A2"
                     /note="hexamer: AATAAA"
     polyA_site      4982
                     /gene="NPC1L1"
                     /gene_synonym="LDLCQ7; NPC11L1; SLC65A2"
                     /note="major polyA site"
ORIGIN      
        1 aatgtgtgtt cagcccttgg ctgttcctga ggcctggcct ggctccccgc tgaccccttc
       61 ccagacctgg gatggcggag gccggcctga ggggctggct gctgtgggcc ctgctcctgc
      121 gcttggccca gagtgagcct tacacaacca tccaccagcc tggctactgc gccttctatg
      181 acgaatgtgg gaagaaccca gagctgtctg gaagcctcat gacactctcc aacgtgtcct
      241 gcctgtccaa cacgccggcc cgcaagatca caggtgatca cctgatccta ttacagaaga
      301 tctgcccccg cctctacacc ggccccaaca cccaagcctg ctgctccgcc aagcagctgg
      361 tatcactgga agcgagtctg tcgatcacca aggccctcct cacccgctgc ccagcctgct
      421 ctgacaattt tgtgaacctg cactgccaca acacgtgcag ccccaatcag agcctcttca
      481 tcaatgtgac ccgcgtggcc cagctagggg ctggacaact cccagctgtg gtggcctatg
      541 aggccttcta ccagcatagc tttgccgagc agagctatga ctcctgcagc cgtgtgcgcg
      601 tccctgcagc tgccacgctg gctgtgggca ccatgtgtgg cgtgtatggc tctgcccttt
      661 gcaatgccca gcgctggctc aacttccagg gagacacagg caatggtctg gccccactgg
      721 acatcacctt ccacctcttg gagcctggcc aggccgtggg gagtgggatt cagcctctga
      781 atgagggggt tgcacgttgc aatgagtccc aaggtgacga cgtggcgacc tgctcctgcc
      841 aagactgtgc tgcatcctgt cctgccatag cccgccccca ggccctcgac tccaccttct
      901 acctgggcca gatgccgggc agtctggtcc tcatcatcat cctctgctct gtcttcgctg
      961 tggtcaccat cctgcttgtg ggattccgtg tggcccccgc cagggacaaa agcaagatgg
     1021 tggaccccaa gaagggcacc agcctctctg acaagctcag cttctccacc cacaccctcc
     1081 ttggccagtt cttccagggc tggggcacgt gggtggcttc gtggcctctg accatcttgg
     1141 tgctatctgt catcccggtg gtggccttgg cagcgggcct ggtctttaca gaactcacta
     1201 cggaccccgt ggagctgtgg tcggccccca acagccaagc ccggagtgag aaagctttcc
     1261 atgaccagca tttcggcccc ttcttccgaa ccaaccaggt gatcctgacg gctcctaacc
     1321 ggtccagcta caggtatgac tctctgctgc tggggcccaa gaacttcagc ggaatcctgg
     1381 acctggactt gctgctggag ctgctagagc tgcaggagag gctgcggcac ctccaggtat
     1441 ggtcgcccga agcacagcgc aacatctccc tgcaggacat ctgctacgcc cccctcaatc
     1501 cggacaatac cagtctctac gactgctgca tcaacagcct cctgcagtat ttccagaaca
     1561 accgcacgct cctgctgctc acagccaacc agacactgat ggggcagacc tcccaagtcg
     1621 actggaagga ccattttctg tactgtgcca atgccccgct caccttcaag gatggcacag
     1681 ccctggccct gagctgcatg gctgactacg gggcccctgt cttccccttc cttgccattg
     1741 gggggtacaa aggaaaggac tattctgagg cagaggccct gatcatgacg ttctccctca
     1801 acaattaccc tgccggggac ccccgtctgg cccaggccaa gctgtgggag gaggccttct
     1861 tagaggaaat gcgagccttc cagcgtcgga tggctggcat gttccaggtc acgttcatgg
     1921 ctgagcgctc tctggaagac gagatcaatc gcaccacagc tgaagacctg cccatctttg
     1981 ccaccagcta cattgtcata ttcctgtaca tctctctggc cctgggcagc tattccagct
     2041 ggagccgagt gatggtggac tccaaggcca cgctgggcct cggcggggtg gccgtggtcc
     2101 tgggagcagt catggctgcc atgggcttct tctcctactt gggtatccgc tcctccctgg
     2161 tcatcctgca agtggttcct ttcctggtgc tgtccgtggg ggctgataac atcttcatct
     2221 ttgttctcga gtaccagagg ctgccccgga ggcctgggga gccacgagag gtccacattg
     2281 ggcgagccct aggcagggtg gctcccagca tgctgttgtg cagcctctct gaggccatct
     2341 gcttcttcct aggggccctg acccccatgc cagctgtgcg gacctttgcc ctgacctctg
     2401 gccttgcagt gatccttgac ttcctcctgc agatgtcagc ctttgtggcc ctgctctccc
     2461 tggacagcaa gaggcaggag gcctcccggt tggacgtctg ctgctgtgtc aagccccagg
     2521 agctgccccc gcctggccag ggagaggggc tcctgcttgg cttcttccaa aaggcttatg
     2581 cccccttcct gctgcactgg atcactcgag gtgttgtgct gctgctgttt ctcgccctgt
     2641 tcggagtgag cctctactcc atgtgccaca tcagcgtggg actggaccag gagctggccc
     2701 tgcccaagga ctcgtacctg cttgactatt tcctctttct gaaccgctac ttcgaggtgg
     2761 gggccccggt gtactttgtt accaccttgg gctacaactt ctccagcgag gctgggatga
     2821 atgccatctg ctccagtgca ggctgcaaca acttctcctt cacccagaag atccagtatg
     2881 ccacagagtt ccctgagcag tcttacctgg ccatccctgc ctcctcctgg gtggatgact
     2941 tcattgactg gctgaccccg tcctcctgct gccgccttta tatatctggc cccaataagg
     3001 acaagttctg cccctcgacc gtcaactctc tgaactgcct aaagaactgc atgagcatca
     3061 cgatgggctc tgtgaggccc tcggtggagc agttccataa gtatcttccc tggttcctga
     3121 acgaccggcc caacatcaaa tgtcccaaag gcggcctggc agcatacagc acctctgtga
     3181 acttgacttc agatggccag gttttagcct ccaggttcat ggcctatcac aagcccctga
     3241 aaaactcaca ggattacaca gaagctctgc gggcagctcg agagctggca gccaacatca
     3301 ctgctgacct gcggaaagtg cctggaacag acccggcttt tgaggtcttc ccctacacga
     3361 tcaccaatgt gttttatgag cagtacctga ccatcctccc tgaggggctc ttcatgctca
     3421 gcctctgcct tgtgcccacc ttcgctgtct cctgcctcct gctgggcctg gacctgcgct
     3481 ccggcctcct caacctgctc tccattgtca tgatcctcgt ggacactgtc ggcttcatgg
     3541 ccctgtgggg catcagttac aatgctgtgt ccctcatcaa cctggtctcg gcggtgggca
     3601 tgtctgtgga gtttgtgtcc cacattaccc gctcctttgc catcagcacc aagcccacct
     3661 ggctggagag ggccaaagag gccaccatct ctatgggaag tgcggtgttt gcaggtgtgg
     3721 ccatgaccaa cctgcctggc atccttgtcc tgggcctcgc caaggcccag ctcattcaga
     3781 tcttcttctt ccgcctcaac ctcctgatca ctctgctggg cctgctgcat ggcttggtct
     3841 tcctgcccgt catcctcagc tacgtggggc ctgacgttaa cccggctctg gcactggagc
     3901 agaagcgggc tgaggaggcg gtggcagcag tcatggtggc ctcttgccca aatcacccct
     3961 cccgagtctc cacagctgac aacatctatg tcaaccacag ctttgaaggt tctatcaaag
     4021 gtgctggtgc catcagcaac ttcttgccca acaatgggcg gcagttctga tacagccaga
     4081 ggccctgtct aggctctatg gccctgaacc aaagggttat ggggatcttc cttgtgactg
     4141 ccccttgaca cacgccctcc tcaaatccta ggggaggcca ttcccatgag actgcctgtc
     4201 actggaggat ggcctgctct tgaggtatcc aggcagcacc actgatggct cctctgctcc
     4261 catagtgggt ccccagtttc caagtcacct aggccttggg cagtgcctcc tcctgggcct
     4321 gggtctggaa gttggcagga acagacacac tccatgtttg tcccacactc actcactttc
     4381 ctaggagccc acttctcatc caacttttcc cttctcagtt cctctctcga aagtcttaat
     4441 tctgtgtcag taagtcttta acacgtagca gtgtccctga gaacacagac aatgaccact
     4501 accctgggtg tgatatcaca ggaggccaga gagaggcaaa ggctcaggcc aagagccaac
     4561 gctgtgggag gccggtcggc agccactccc tccagggcgc acctgcaggt ctgccatcca
     4621 cggccttttc tggcaagaga agggcccagg aaggatgctc tcataaggcc caggaaggat
     4681 gctctcataa gcaccttggt catggattag cccctcctgg aaaatggtgt tgggtttggt
     4741 ctccagctcc aatacttatt aaggctgttg ctgccagtca aggccaccca ggagtctgaa
     4801 ggctgggagc tcttggggct gggctggtcc tcccatcttc acctcgggcc tggatcccag
     4861 gcctcaaacc agcccaaccc gagcttttgg acagctctcc agaagcatga actgcagtgg
     4921 agatgaagat cctggctctg tgctgtgcac ataggtgttt aataaacatt tgttggcaga
     4981 aa
//
