LOCUS       NM_005286               3323 bp    mRNA    linear   PRI 07-APR-2024
DEFINITION  Homo sapiens neuropeptides B and W receptor 2 (NPBWR2), mRNA.
ACCESSION   NM_005286
VERSION     NM_005286.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3323)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   2  (bases 1 to 3323)
  AUTHORS   Bu,G., Lin,D., Cui,L., Huang,L., Lv,C., Huang,S., Wan,Y., Fang,C.,
            Li,J. and Wang,Y.
  TITLE     Characterization of Neuropeptide B (NPB), Neuropeptide W (NPW), and
            Their Receptors in Chickens: Evidence for NPW Being a Novel
            Inhibitor of Pituitary GH and Prolactin Secretion
  JOURNAL   Endocrinology 157 (9), 3562-3576 (2016)
   PUBMED   27399877
  REMARK    GeneRIF: mainly expressed in brain or pituitary
REFERENCE   3  (bases 1 to 3323)
  AUTHORS   Luttrell,L.M.
  TITLE     Reviews in molecular biology and biotechnology: transmembrane
            signaling by G protein-coupled receptors
  JOURNAL   Mol Biotechnol 39 (3), 239-264 (2008)
   PUBMED   18240029
  REMARK    Review article
REFERENCE   4  (bases 1 to 3323)
  AUTHORS   Lambert,N.A.
  TITLE     Dissociation of heterotrimeric g proteins in cells
  JOURNAL   Sci Signal 1 (25), re5 (2008)
   PUBMED   18577758
  REMARK    Review article
            Erratum:[Sci Signal. 2008 Jul 15;1(28):er3]
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 3323)
  AUTHORS   Mazzocchi,G., Rebuffat,P., Ziolkowska,A., Rossi,G.P.,
            Malendowicz,L.K. and Nussdorfer,G.G.
  TITLE     G protein receptors 7 and 8 are expressed in human adrenocortical
            cells, and their endogenous ligands neuropeptides B and w enhance
            cortisol secretion by activating adenylate cyclase- and
            phospholipase C-dependent signaling cascades
  JOURNAL   J Clin Endocrinol Metab 90 (6), 3466-3471 (2005)
   PUBMED   15797961
  REMARK    GeneRIF: Expressed in the zona glomerulosa and zona
            fasciculata-reticularis ( cells of the human adrenal cortex.
REFERENCE   6  (bases 1 to 3323)
  AUTHORS   Brezillon,S., Lannoy,V., Franssen,J.D., Le Poul,E., Dupriez,V.,
            Lucchetti,J., Detheux,M. and Parmentier,M.
  TITLE     Identification of natural ligands for the orphan G protein-coupled
            receptors GPR7 and GPR8
  JOURNAL   J Biol Chem 278 (2), 776-783 (2003)
   PUBMED   12401809
  REMARK    GeneRIF: distribution in central nervous system and peripheral
            tissues with precursor peptides
REFERENCE   7  (bases 1 to 3323)
  AUTHORS   Shimomura,Y., Harada,M., Goto,M., Sugo,T., Matsumoto,Y., Abe,M.,
            Watanabe,T., Asami,T., Kitada,C., Mori,M., Onda,H. and Fujino,M.
  TITLE     Identification of neuropeptide W as the endogenous ligand for
            orphan G-protein-coupled receptors GPR7 and GPR8
  JOURNAL   J Biol Chem 277 (39), 35826-35832 (2002)
   PUBMED   12130646
REFERENCE   8  (bases 1 to 3323)
  AUTHORS   Deloukas,P., Matthews,L.H., Ashurst,J., Burton,J., Gilbert,J.G.,
            Jones,M., Stavrides,G., Almeida,J.P., Babbage,A.K., Bagguley,C.L.,
            Bailey,J., Barlow,K.F., Bates,K.N., Beard,L.M., Beare,D.M.,
            Beasley,O.P., Bird,C.P., Blakey,S.E., Bridgeman,A.M., Brown,A.J.,
            Buck,D., Burrill,W., Butler,A.P., Carder,C., Carter,N.P.,
            Chapman,J.C., Clamp,M., Clark,G., Clark,L.N., Clark,S.Y.,
            Clee,C.M., Clegg,S., Cobley,V.E., Collier,R.E., Connor,R.,
            Corby,N.R., Coulson,A., Coville,G.J., Deadman,R., Dhami,P.,
            Dunn,M., Ellington,A.G., Frankland,J.A., Fraser,A., French,L.,
            Garner,P., Grafham,D.V., Griffiths,C., Griffiths,M.N., Gwilliam,R.,
            Hall,R.E., Hammond,S., Harley,J.L., Heath,P.D., Ho,S., Holden,J.L.,
            Howden,P.J., Huckle,E., Hunt,A.R., Hunt,S.E., Jekosch,K.,
            Johnson,C.M., Johnson,D., Kay,M.P., Kimberley,A.M., King,A.,
            Knights,A., Laird,G.K., Lawlor,S., Lehvaslaiho,M.H., Leversha,M.,
            Lloyd,C., Lloyd,D.M., Lovell,J.D., Marsh,V.L., Martin,S.L.,
            McConnachie,L.J., McLay,K., McMurray,A.A., Milne,S., Mistry,D.,
            Moore,M.J., Mullikin,J.C., Nickerson,T., Oliver,K., Parker,A.,
            Patel,R., Pearce,T.A., Peck,A.I., Phillimore,B.J.,
            Prathalingam,S.R., Plumb,R.W., Ramsay,H., Rice,C.M., Ross,M.T.,
            Scott,C.E., Sehra,H.K., Shownkeen,R., Sims,S., Skuce,C.D.,
            Smith,M.L., Soderlund,C., Steward,C.A., Sulston,J.E., Swann,M.,
            Sycamore,N., Taylor,R., Tee,L., Thomas,D.W., Thorpe,A., Tracey,A.,
            Tromans,A.C., Vaudin,M., Wall,M., Wallis,J.M., Whitehead,S.L.,
            Whittaker,P., Willey,D.L., Williams,L., Williams,S.A., Wilming,L.,
            Wray,P.W., Hubbard,T., Durbin,R.M., Bentley,D.R., Beck,S. and
            Rogers,J.
  TITLE     The DNA sequence and comparative analysis of human chromosome 20
  JOURNAL   Nature 414 (6866), 865-871 (2001)
   PUBMED   11780052
REFERENCE   9  (bases 1 to 3323)
  AUTHORS   O'Dowd,B.F., Scheideler,M.A., Nguyen,T., Cheng,R., Rasmussen,J.S.,
            Marchese,A., Zastawny,R., Heng,H.H., Tsui,L.C., Shi,X. et al.
  TITLE     The cloning and chromosomal mapping of two novel human
            opioid-somatostatin-like receptor genes, GPR7 and GPR8, expressed
            in discrete areas of the brain
  JOURNAL   Genomics 28 (1), 84-91 (1995)
   PUBMED   7590751
REFERENCE   10 (bases 1 to 3323)
  AUTHORS   Itoh,H., Toyama,R., Kozasa,T., Tsukamoto,T., Matsuoka,M. and
            Kaziro,Y.
  TITLE     Presence of three distinct molecular species of Gi protein alpha
            subunit. Structure of rat cDNAs and human genomic DNAs
  JOURNAL   J Biol Chem 263 (14), 6656-6664 (1988)
   PUBMED   2834384
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL121581.41.
            
            On Dec 30, 2019 this sequence version replaced NM_005286.3.
            
            Summary: The protein encoded by this gene is an integral membrane
            protein and G protein-coupled receptor. The encoded protein is
            similar in sequence to another G protein-coupled receptor (GPR7),
            and it is structurally similar to opioid and somatostatin
            receptors. This protein binds neuropeptides B and W. This gene is
            intronless and is expressed primarily in the frontal cortex of the
            brain. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038194.2030481.1,
                                           SRR14038192.3861302.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2145743, SAMEA2157437
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000684052.1/ ENSP00000508236.1
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-202               AL121581.41        12410-12611         c
            203-3323            AL121581.41        8848-11968          c
FEATURES             Location/Qualifiers
     source          1..3323
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q13.33"
     gene            1..3323
                     /gene="NPBWR2"
                     /gene_synonym="GPR8"
                     /note="neuropeptides B and W receptor 2"
                     /db_xref="GeneID:2832"
                     /db_xref="HGNC:HGNC:4530"
                     /db_xref="MIM:600731"
     exon            1..202
                     /gene="NPBWR2"
                     /gene_synonym="GPR8"
                     /inference="alignment:Splign:2.1.0"
     exon            203..3323
                     /gene="NPBWR2"
                     /gene_synonym="GPR8"
                     /inference="alignment:Splign:2.1.0"
     CDS             294..1295
                     /gene="NPBWR2"
                     /gene_synonym="GPR8"
                     /note="opioid-somatostatin-like receptor 8; G-protein
                     coupled receptor 8; neuropeptides B/W receptor 2"
                     /codon_start=1
                     /product="neuropeptides B/W receptor type 2"
                     /protein_id="NP_005277.2"
                     /db_xref="CCDS:CCDS13557.1"
                     /db_xref="GeneID:2832"
                     /db_xref="HGNC:HGNC:4530"
                     /db_xref="MIM:600731"
                     /translation="MQAAGHPEPLDSRGSFSLPTMGANVSQDNGTGHNATFSEPLPFL
                     YVLLPAVYSGICAVGLTGNTAVILVILRAPKMKTVTNVFILNLAVADGLFTLVLPVNI
                     AEHLLQYWPFGELLCKLVLAVDHYNIFSSIYFLAVMSVDRYLVVLATVRSRHMPWRTY
                     RGAKVASLCVWLGVTVLVLPFFSFAGVYSNELQVPSCGLSFPWPEQVWFKASRVYTLV
                     LGFVLPVCTICVLYTDLLRRLRAVRLRSGAKALGKARRKVTVLVLVVLAVCLLCWTPF
                     HLASVVALTTDLPQTPLVISMSYVITSLSYANSCLNPFLYAFLDDNFRKNFRSILRC"
     regulatory      3303..3308
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NPBWR2"
                     /gene_synonym="GPR8"
                     /note="hexamer: AACAAA"
     polyA_site      3323
                     /gene="NPBWR2"
                     /gene_synonym="GPR8"
                     /note="major polyA site"
ORIGIN      
        1 agcatcagca ctgacaccag gctgggaacc tcgcagccgg ggctgaggca gccagcaggg
       61 caggtcttga aactcctgaa acctcagctg gaggtctgtc agtgaggacc acacctcgcc
      121 ctccatccct gcgactgcct gtactggcca aaaccaggaa cacaccaagg ctaccaactt
      181 ctggactcga agcactggac agaatccagc ccaaggaggc ccccaaccag atacccaact
      241 ccaaggcacc tcccacctgc ccagggcgca aatcgtcaac ggtcccagct acaatgcagg
      301 ccgctgggca cccagagccc cttgacagca ggggctcctt ctccctcccc acgatgggtg
      361 ccaacgtctc tcaggacaat ggcactggcc acaatgccac cttctccgag ccactgccgt
      421 tcctctatgt gctcctgccc gccgtgtact ccgggatctg tgctgtgggg ctgactggca
      481 acacggccgt catccttgta atcctaaggg cgcccaagat gaagacggtg accaacgtgt
      541 tcatcctgaa cctggccgtc gccgacgggc tcttcacgct ggtactgccc gtcaacatcg
      601 cggagcacct gctgcagtac tggcccttcg gggagctgct ctgcaagctg gtgctggccg
      661 tcgaccacta caacatcttc tccagcatct acttcctagc cgtgatgagc gtggaccgat
      721 acctggtggt gctggccacc gtgaggtccc gccacatgcc ctggcgcacc taccgggggg
      781 cgaaggtcgc cagcctgtgt gtctggctgg gcgtcacggt cctggttctg cccttcttct
      841 ctttcgctgg cgtctacagc aacgagctgc aggtcccaag ctgtgggctg agcttcccgt
      901 ggcccgagca ggtctggttc aaggccagcc gtgtctacac gttggtcctg ggcttcgtgc
      961 tgcccgtgtg caccatctgt gtgctctaca cagacctcct gcgcaggctg cgggccgtgc
     1021 ggctccgctc tggagccaag gctctaggca aggccaggcg gaaggtgacc gtcctggtcc
     1081 tcgtcgtgct ggccgtgtgc ctcctctgct ggacgccctt ccacctggcc tctgtcgtgg
     1141 ccctgaccac ggacctgccc cagaccccac tggtcatcag tatgtcctac gtcatcacca
     1201 gcctcagcta cgccaactcg tgcctgaacc ccttcctcta cgcctttcta gatgacaact
     1261 tccggaagaa cttccgcagc atattgcggt gctgaagggc ctgggcacca tcacccccat
     1321 catcatgccc atcatcaccc cccatcatca tcacgcccat catcatcatg cccacacccc
     1381 catcaggccc accccccccc acccccacca ccacccccac catcaccccc accaccaccc
     1441 ccatcatcac gcccatcatc atgcccaccc ccatcacatc caccatcacg cccaccccca
     1501 tcacccccac ccccaccatc acgcccacca tcacccccac ccccaccccc acccccatca
     1561 tcacgcccac cccccccacc acccccacca tcacccccat catcacgccc acccccaccc
     1621 agttccggct gtctctttca ggggacgccc ggcctgttca catcccagtg ctgtcctctc
     1681 cacgcaagga gacacagacc ctcacacgct ggccaccaga cctacagaca gccgtgggca
     1741 tatacctccc atgtctctgc ttcctggaca gcccctgggt ggacagcagc agggatccag
     1801 gtcctggcag aggcccccac acagcaagca tcccgggccc acttacccac gagagacaga
     1861 cagatggaca agccccttgg aaggctctgg gactcaggat ccctaatgac agtggtcggg
     1921 aggggaggga acctgaactg tgatcttgac ctgggccacg ttacatagtc tctccccaag
     1981 atggggccgg ggggtggtaa gtgcgggcac ctcaggcagc agcgatgctg tgttgctgca
     2041 aaggcggaca cctgcaccgc atgagaccca gaaatccact ctcgtggcct gaagcccaag
     2101 tggagttcca gcaggaggcc cagccccgcc gtgcccatgt gccatggcct gtgctgctgt
     2161 gttccccctg ctgtggccga cggtccttgc catggcctgt acccgccctg ctgtgtccga
     2221 cagtcctctc catggcctgt gctcccctgt gcccccctgc tgtgtccgac ggtcctctcc
     2281 atggcctgtg ctcccctgta cccccctgct gtggccgaca gtcctcacca tggcctgtgc
     2341 tcccctgtgc ccccctgctg tggccgacag tcctcaccat ggcctgtgct cccctatact
     2401 cccctgctgt ggccgacggt cctctccatg gcctgtgctc ccctgtaccc ccctgctgtg
     2461 tctgacggtc ctctccatgg cctgtgctcc cctatactcc cctgctgtgg ccgacggtcc
     2521 tctccatggc ctgtgctccc ctgctgtggc cgacagtcct caccatggcc tgtgctcccc
     2581 tgtgcccccc tgctgtgtct gacggtcctc tccatggcct gtgctcccct gtacccccct
     2641 gctgtggccg acggtcctcg ccatggcctg tacacccctg ctgtggccga tgctcctctc
     2701 atactttact agaggctaca gggccctttt gccaggacct ctgggccacc acagcctagg
     2761 gaccatccat cacttggaca ggctggcacc tggaactctc tctcccgggg tgtggccagg
     2821 ggcttgtaat cagccagcag gagctgcatg ctgaaactct ggtgggggca gggtgagggg
     2881 tggtaatgaa atgcaggcag ttcagagacc gctgactgtc aggtgggagg ggccaggggt
     2941 tggtcccagg tgtgctgtgg ccaggccccg actcttggcc ccatggctct cagctggttc
     3001 catgcctggc attccagcct ggcagtgagg tgtgagtgtt ctgggttggt ttcagtcctc
     3061 atgacccaga gctctgatca atctgtccag ccggcacaca ggacccacca ttcaccgccc
     3121 cacgaggtgt cccagggtgt atgcccacct ccacccatcc accaaatact ccagcgttcc
     3181 agcccactcg gctcaggcct gcagcagtgc ccctcctccg tgctagccag ccaaacacgc
     3241 cacggccatc tccatccact tatcctccct gacaacattg tattagatat ctgttgctga
     3301 ataacaaatt tctccaaaac tta
//
