LOCUS       NM_016931               4269 bp    mRNA    linear   PRI 17-SEP-2024
DEFINITION  Homo sapiens NADPH oxidase 4 (NOX4), transcript variant 1, mRNA.
ACCESSION   NM_016931
VERSION     NM_016931.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4269)
  AUTHORS   Alves,J.V., da Costa,R.M., Awata,W.M.C., Bruder-Nascimento,A.,
            Singh,S., Tostes,R.C. and Bruder-Nascimento,T.
  TITLE     NADPH oxidase 4-derived hydrogen peroxide counterbalances
            testosterone-induced endothelial dysfunction and migration
  JOURNAL   Am J Physiol Endocrinol Metab 327 (1), E1-E12 (2024)
   PUBMED   38690939
  REMARK    GeneRIF: NADPH oxidase 4-derived hydrogen peroxide counterbalances
            testosterone-induced endothelial dysfunction and migration.
REFERENCE   2  (bases 1 to 4269)
  AUTHORS   Wang,J., Lai,D.A., Wang,J.J. and Zhang,S.X.
  TITLE     Effects of Nox4 upregulation on PECAM-1 expression in a mouse model
            of diabetic retinopathy
  JOURNAL   PLoS One 19 (5), e0303010 (2024)
   PUBMED   38748682
  REMARK    GeneRIF: Effects of Nox4 upregulation on PECAM-1 expression in a
            mouse model of diabetic retinopathy.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 4269)
  AUTHORS   Wang,L. and Gong,W.
  TITLE     NOX4 regulates gastric cancer cell invasion and proliferation by
            increasing ferroptosis sensitivity through regulating ROS
  JOURNAL   Int Immunopharmacol 132, 112052 (2024)
   PUBMED   38593505
  REMARK    GeneRIF: NOX4 regulates gastric cancer cell invasion and
            proliferation by increasing ferroptosis sensitivity through
            regulating ROS.
REFERENCE   4  (bases 1 to 4269)
  AUTHORS   Liu,Y., Huang,H., Yang,X., Huang,D., Wang,X., Yuan,M. and Hong,L.
  TITLE     Comprehensive bioinformatics analysis of NOX4 as a biomarker for
            pan-cancer prognosis and immune infiltration
  JOURNAL   Aging (Albany NY) 16 (8), 7437-7447 (2024)
   PUBMED   38663913
  REMARK    GeneRIF: Comprehensive bioinformatics analysis of NOX4 as a
            biomarker for pan-cancer prognosis and immune infiltration.
REFERENCE   5  (bases 1 to 4269)
  AUTHORS   Yu,W., Zhuang,S., Zhan,M., Chen,Y., Zhang,J., Chen,L., Tu,C.,
            Zheng,L. and Chen,S.
  TITLE     Deubiquitinating Enzyme USP19 Regulates Ferroptosis and
            Mitochondrial Damage in SH-SY5Y Cells by Targeting the NOX4 Protein
  JOURNAL   J Alzheimers Dis 100 (3), 799-808 (2024)
   PUBMED   38943386
  REMARK    GeneRIF: Deubiquitinating Enzyme USP19 Regulates Ferroptosis and
            Mitochondrial Damage in SH-SY5Y Cells by Targeting the NOX4
            Protein.
REFERENCE   6  (bases 1 to 4269)
  AUTHORS   Bedard,K. and Krause,K.H.
  TITLE     The NOX family of ROS-generating NADPH oxidases: physiology and
            pathophysiology
  JOURNAL   Physiol Rev 87 (1), 245-313 (2007)
   PUBMED   17237347
  REMARK    Review article
REFERENCE   7  (bases 1 to 4269)
  AUTHORS   Brar,S.S., Kennedy,T.P., Sturrock,A.B., Huecksteadt,T.P.,
            Quinn,M.T., Whorton,A.R. and Hoidal,J.R.
  TITLE     An NAD(P)H oxidase regulates growth and transcription in melanoma
            cells
  JOURNAL   Am J Physiol Cell Physiol 282 (6), C1212-C1224 (2002)
   PUBMED   11997235
REFERENCE   8  (bases 1 to 4269)
  AUTHORS   Cheng,G., Cao,Z., Xu,X., van Meir,E.G. and Lambeth,J.D.
  TITLE     Homologs of gp91phox: cloning and tissue expression of Nox3, Nox4,
            and Nox5
  JOURNAL   Gene 269 (1-2), 131-140 (2001)
   PUBMED   11376945
REFERENCE   9  (bases 1 to 4269)
  AUTHORS   Shiose,A., Kuroda,J., Tsuruya,K., Hirai,M., Hirakata,H., Naito,S.,
            Hattori,M., Sakaki,Y. and Sumimoto,H.
  TITLE     A novel superoxide-producing NAD(P)H oxidase in kidney
  JOURNAL   J Biol Chem 276 (2), 1417-1423 (2001)
   PUBMED   11032835
REFERENCE   10 (bases 1 to 4269)
  AUTHORS   Geiszt,M., Kopp,J.B., Varnai,P. and Leto,T.L.
  TITLE     Identification of renox, an NAD(P)H oxidase in kidney
  JOURNAL   Proc Natl Acad Sci U S A 97 (14), 8010-8014 (2000)
   PUBMED   10869423
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AP002404.2 and AP001815.4.
            
            On Nov 23, 2018 this sequence version replaced NM_016931.4.
            
            Summary: This gene encodes a member of the NOX-family of enzymes
            that functions as the catalytic subunit the NADPH oxidase complex.
            The encoded protein is localized to non-phagocytic cells where it
            acts as an oxygen sensor and catalyzes the reduction of molecular
            oxygen to various reactive oxygen species (ROS). The ROS generated
            by this protein have been implicated in numerous biological
            functions including signal transduction, cell differentiation and
            tumor cell growth. A pseudogene has been identified on the other
            arm of chromosome 11. Alternative splicing results in multiple
            transcript variants.[provided by RefSeq, Jan 2009].
            
            Transcript Variant: This variant (1) encodes isoform (a).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB041035.1, BC040105.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000263317.9/ ENSP00000263317.4
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-186               AP002404.2         26123-26308         c
            187-282             AP002404.2         25391-25486         c
            283-393             AP001815.4         144607-144717       c
            394-478             AP001815.4         142262-142346       c
            479-576             AP001815.4         136957-137054       c
            577-604             AP001815.4         133510-133537       c
            605-677             AP001815.4         125606-125678       c
            678-758             AP001815.4         114724-114804       c
            759-975             AP001815.4         95148-95364         c
            976-1140            AP001815.4         93037-93201         c
            1141-1203           AP001815.4         92839-92901         c
            1204-1264           AP001815.4         66254-66314         c
            1265-1346           AP001815.4         47784-47865         c
            1347-1466           AP001815.4         34896-35015         c
            1467-1575           AP001815.4         32885-32993         c
            1576-1644           AP001815.4         30269-30337         c
            1645-1745           AP001815.4         28667-28767         c
            1746-4269           AP001815.4         17175-19698         c
FEATURES             Location/Qualifiers
     source          1..4269
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q14.3"
     gene            1..4269
                     /gene="NOX4"
                     /gene_synonym="KOX; KOX-1; RENOX"
                     /note="NADPH oxidase 4"
                     /db_xref="GeneID:50507"
                     /db_xref="HGNC:HGNC:7891"
                     /db_xref="MIM:605261"
     exon            1..186
                     /gene="NOX4"
                     /gene_synonym="KOX; KOX-1; RENOX"
                     /inference="alignment:Splign:2.1.0"
     CDS             130..1866
                     /gene="NOX4"
                     /gene_synonym="KOX; KOX-1; RENOX"
                     /EC_number="1.6.3.1"
                     /note="isoform a is encoded by transcript variant 1;
                     kidney superoxide-producing NADPH oxidase; renal
                     NAD(P)H-oxidase; kidney oxidase-1"
                     /codon_start=1
                     /product="NADPH oxidase 4 isoform a"
                     /protein_id="NP_058627.2"
                     /db_xref="CCDS:CCDS8285.1"
                     /db_xref="GeneID:50507"
                     /db_xref="HGNC:HGNC:7891"
                     /db_xref="MIM:605261"
                     /translation="MAVSWRSWLANEGVKHLCLFIWLSMNVLLFWKTFLLYNQGPEYH
                     YLHQMLGLGLCLSRASASVLNLNCSLILLPMCRTLLAYLRGSQKVPSRRTRRLLDKSR
                     TFHITCGVTICIFSGVHVAAHLVNALNFSVNYSEDFVELNAARYRDEDPRKLLFTTVP
                     GLTGVCMVVVLFLMITASTYAIRVSNYDIFWYTHNLFFVFYMLLTLHVSGGLLKYQTN
                     LDTHPPGCISLNRTSSQNISLPEYFSEHFHEPFPEGFSKPAEFTQHKFVKICMEEPRF
                     QANFPQTWLWISGPLCLYCAERLYRYIRSNKPVTIISVMSHPSDVMEIRMVKENFKAR
                     PGQYITLHCPSVSALENHPFTLTMCPTETKATFGVHLKIVGDWTERFRDLLLPPSSQD
                     SEILPFIQSRNYPKLYIDGPFGSPFEESLNYEVSLCVAGGIGVTPFASILNTLLDDWK
                     PYKLRRLYFIWVCRDIQSFRWFADLLCMLHNKFWQENRPDYVNIQLYLSQTDGIQKII
                     GEKYHALNSRLFIGRPRWKLLFDEIAKYNRGKTVGVFCCGPNSLSKTLHKLSNQNNSY
                     GTRFEYNKESFS"
     exon            187..282
                     /gene="NOX4"
                     /gene_synonym="KOX; KOX-1; RENOX"
                     /inference="alignment:Splign:2.1.0"
     exon            283..393
                     /gene="NOX4"
                     /gene_synonym="KOX; KOX-1; RENOX"
                     /inference="alignment:Splign:2.1.0"
     exon            394..478
                     /gene="NOX4"
                     /gene_synonym="KOX; KOX-1; RENOX"
                     /inference="alignment:Splign:2.1.0"
     exon            479..576
                     /gene="NOX4"
                     /gene_synonym="KOX; KOX-1; RENOX"
                     /inference="alignment:Splign:2.1.0"
     exon            577..604
                     /gene="NOX4"
                     /gene_synonym="KOX; KOX-1; RENOX"
                     /inference="alignment:Splign:2.1.0"
     exon            605..677
                     /gene="NOX4"
                     /gene_synonym="KOX; KOX-1; RENOX"
                     /inference="alignment:Splign:2.1.0"
     exon            678..758
                     /gene="NOX4"
                     /gene_synonym="KOX; KOX-1; RENOX"
                     /inference="alignment:Splign:2.1.0"
     exon            759..975
                     /gene="NOX4"
                     /gene_synonym="KOX; KOX-1; RENOX"
                     /inference="alignment:Splign:2.1.0"
     exon            976..1140
                     /gene="NOX4"
                     /gene_synonym="KOX; KOX-1; RENOX"
                     /inference="alignment:Splign:2.1.0"
     exon            1141..1203
                     /gene="NOX4"
                     /gene_synonym="KOX; KOX-1; RENOX"
                     /inference="alignment:Splign:2.1.0"
     exon            1204..1264
                     /gene="NOX4"
                     /gene_synonym="KOX; KOX-1; RENOX"
                     /inference="alignment:Splign:2.1.0"
     exon            1265..1346
                     /gene="NOX4"
                     /gene_synonym="KOX; KOX-1; RENOX"
                     /inference="alignment:Splign:2.1.0"
     exon            1347..1466
                     /gene="NOX4"
                     /gene_synonym="KOX; KOX-1; RENOX"
                     /inference="alignment:Splign:2.1.0"
     exon            1467..1575
                     /gene="NOX4"
                     /gene_synonym="KOX; KOX-1; RENOX"
                     /inference="alignment:Splign:2.1.0"
     exon            1576..1644
                     /gene="NOX4"
                     /gene_synonym="KOX; KOX-1; RENOX"
                     /inference="alignment:Splign:2.1.0"
     exon            1645..1745
                     /gene="NOX4"
                     /gene_synonym="KOX; KOX-1; RENOX"
                     /inference="alignment:Splign:2.1.0"
     exon            1746..4269
                     /gene="NOX4"
                     /gene_synonym="KOX; KOX-1; RENOX"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2255..2260
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NOX4"
                     /gene_synonym="KOX; KOX-1; RENOX"
                     /note="hexamer: ATTAAA"
     polyA_site      2278
                     /gene="NOX4"
                     /gene_synonym="KOX; KOX-1; RENOX"
                     /note="major polyA site"
     regulatory      2337..2342
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NOX4"
                     /gene_synonym="KOX; KOX-1; RENOX"
                     /note="hexamer: AATATA"
     polyA_site      2361
                     /gene="NOX4"
                     /gene_synonym="KOX; KOX-1; RENOX"
     regulatory      4250..4255
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NOX4"
                     /gene_synonym="KOX; KOX-1; RENOX"
                     /note="hexamer: AATAAA"
     polyA_site      4269
                     /gene="NOX4"
                     /gene_synonym="KOX; KOX-1; RENOX"
ORIGIN      
        1 actcagtctt tgaccctcgg tcctcgctca gcggcccggc aggccgcaca actgtaaccg
       61 ctgccccggc cgccgcccgc tccttctcgg tccggcgggc acagagcgca gcgcggcggg
      121 gccggcggca tggctgtgtc ctggaggagc tggctcgcca acgaaggggt taaacacctc
      181 tgcctgttca tctggctctc catgaatgtc ctgcttttct ggaaaacctt cttgctgtat
      241 aaccaagggc cagagtatca ctacctccac cagatgttgg ggctaggatt gtgtctaagc
      301 agagcctcag catctgttct taacctcaac tgcagcctta tccttttacc catgtgccga
      361 acactcttgg cttacctccg aggatcacag aaggttccaa gcaggagaac caggagattg
      421 ttggataaaa gcagaacatt ccatattacc tgtggtgtta ctatctgtat tttctcaggc
      481 gtgcatgtgg ctgcccatct ggtgaatgcc ctcaacttct cagtgaatta cagtgaagac
      541 tttgttgaac tgaatgcagc aagataccga gatgaggatc ctagaaaact tctcttcaca
      601 actgttcctg gcctgacagg ggtctgcatg gtggtggtgc tattcctcat gatcacagcc
      661 tctacatatg caataagagt ttctaactat gatatcttct ggtatactca taacctcttc
      721 tttgtcttct acatgctgct gacgttgcat gtttcaggag ggctgctgaa gtatcaaact
      781 aatttagata cccaccctcc cggctgcatc agtcttaacc gaaccagctc tcagaatatt
      841 tccttaccag agtatttctc agaacatttt catgaacctt tccctgaagg attttcaaaa
      901 ccggcagagt ttacccagca caaatttgtg aagatttgta tggaagagcc cagattccaa
      961 gctaattttc cacagacttg gctttggatt tctggacctt tgtgcctgta ctgtgccgaa
     1021 agactttaca ggtatatccg gagcaataag ccagtcacca tcatttcggt catgagtcat
     1081 ccctcagatg tcatggaaat ccgaatggtc aaagaaaatt ttaaagcaag acctggtcag
     1141 tatattactc tacattgtcc cagtgtatct gcattagaaa atcatccatt taccctcaca
     1201 atgtgtccaa ctgaaaccaa agcaacattt ggggttcatc ttaaaatagt aggagactgg
     1261 acagaacgat ttcgagattt actactgcct ccatctagtc aagactccga aattctgccc
     1321 ttcattcaat ctagaaatta tcccaagctg tatattgatg gtccttttgg aagtccattt
     1381 gaggaatcac tgaactatga ggtcagcctc tgcgtggctg gaggcattgg agtaactcca
     1441 tttgcatcaa tactcaacac cctgttggat gactggaaac catacaagct tagaagacta
     1501 tactttattt gggtatgcag agatatccag tccttccgtt ggtttgcaga tttactctgt
     1561 atgttgcata acaagttttg gcaagagaac agacctgact atgtcaacat ccagctgtac
     1621 ctcagtcaaa cagatgggat acagaagata attggagaaa aatatcatgc actgaattca
     1681 agactgttta taggacgtcc tcggtggaaa cttttgtttg atgaaatagc aaaatataac
     1741 agaggaaaaa cagttggtgt tttctgttgt ggacccaatt cactatccaa gactcttcat
     1801 aaactgagta accagaacaa ctcatatggg acaagatttg aatacaataa agagtctttc
     1861 agctgaaaac ttttgccatg aagcaggact ctaaagaagg aatgagtgca atttctaaga
     1921 ctttgaaact cagcggaatc aatcagctgt gttatgccaa agaatagtaa ggttttctta
     1981 tttatgatta tttaaaatgg aaatgtgaga atgtggcaag atgaccgtca cattacatgt
     2041 ttaatctgga aaccaaagag accctgaaga atatttgatg tgatgattca cttttcagtt
     2101 ctcaaattaa aagaaaactg ttagatgcac actgttgatt ttcatggtgg attcaagaac
     2161 tccctagtga ggagctgaac ttgctcaatc taaggctgat tgtcgtgttc ctctttaaat
     2221 tgtttttggt tgaacaaatg caagattgaa caaaattaaa aattcattga agctgaaatt
     2281 ccattttctg tgttgtgtat aaacagagta gctttaattt gcaagcactc caggcaaata
     2341 tattagatgt ttgaaaacac agcacaagac tctgtattga tacgggtact ttgtgtcaat
     2401 atctaatcgt ctccactact tatgctaata cctctatttg atatctgaag actatatgct
     2461 aactgaacct tcctcaaatg ttgttatagt atctattttt atatattttt ttctttttat
     2521 tcctctctct agggaaatat gccttccctt agcatgcatt agacataatg atttaatagg
     2581 tccctttcat cttcatttaa atctatcact attgcatggt aatgaaaata ttcctactat
     2641 aaattataaa gggatatata tatatggata tatatatgta tatacacata tatatataca
     2701 cacacacaca catatatata tatatatata tatatacaca tatactaata acttttccct
     2761 tttttcagca tttttgtctc tattattatt attgtttttt tcccaggtag ggtttgtctt
     2821 aggctgtagc ctctaaggat agttagttaa tttgcacttt gagaccaaag gacatcatgt
     2881 gtgtcagtag ggactgaata taagatttat ctcctttgcc acacattggt ttatgatgga
     2941 gacattgaaa gtctagtcat attcctgaac agtaaaacct gtattttacc ttttaagtaa
     3001 gaggaaatat gatattctta ttcaaactta agtttagaat ccagaatatt actgtcgcac
     3061 tttttggtat cctgagtttc catagggaac tattgggttt aaagtcaccg ttggaactac
     3121 actgtgtgat cttataaact tatgttccct ggctatcata ttcttggctc agaacaatat
     3181 ttcccattac tatcttaaga attaaggcat tcatggctca cgcctgtaat cccggcactt
     3241 tgggaggcca aggcaggtgg atcacgaggt cagagttcaa gaccagcctg accaagttgg
     3301 tgaaacccca tcgctactaa aaatacaaat attaggcggg tgtggtggcg ggtgcctata
     3361 atcccaacta ctcgggagac tgaggcaaag aatcgcttga acccggtggg cggaggttgc
     3421 agtgagccaa gattgcacca ctgcactcca gcctgggtga cagagcaaga ttccatctca
     3481 aaaaaaaaaa aaaaaaaaaa aaaaaagaat taaagcattc atacagttta gtgattttgt
     3541 ttagtagtcg gctatcaatt gctatcaaat ataacactgc tgaaatcagc agtgtgactt
     3601 accttgccat tgttaaaatg ttacataaaa cataacatga tagatgctaa ggcctttttt
     3661 tgctataatt caccaatagc aatcaagcat gctaacccat actgaatgat atttacttgt
     3721 agatatttct tcctttcctt gaaattctcc tttctatgga aagaagatga acccaaaaaa
     3781 gtgataggaa atgtggaatg ctcatgcaga tttagctctg aaggcatatt taataactag
     3841 tatgtcttga caacagtctt tagattaaaa agaattttca tggaaacatt taacagaaag
     3901 aactagtaaa aagacacttt gagttagtcc aggcttaatg tgcaatactg actctatact
     3961 gatcataatt tatttatgcg atcatattaa tagacctaat ttcattaaaa caggtagaag
     4021 atttttcaaa agaaagatga tgtttcaaag ctggtctgcc attctagatg agcctccttg
     4081 cttatttaag ttccagtagg tgttcaaatg ttaaatgtta aacataggtc atctttgctt
     4141 ctgcagggct tcatcttgca tgtttaagag aactttgttt tattttgagg gattatttct
     4201 ctggggatca ttcttataat acaagcctta aatcactaat tttagtagca ataaatgtta
     4261 aaattgaaa
//
