LOCUS       NM_178493               2223 bp    mRNA    linear   PRI 01-AUG-2024
DEFINITION  Homo sapiens notum, palmitoleoyl-protein carboxylesterase (NOTUM),
            mRNA.
ACCESSION   NM_178493
VERSION     NM_178493.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2223)
  AUTHORS   Zhao,Q., Ren,H., Wang,N., Yuan,X., Zhao,Y. and Wen,Q.
  TITLE     NOTUM plays a bidirectionally modulatory role in the odontoblastic
            differentiation of human stem cells from the apical papilla through
            the WNT/beta-catenin signaling pathway
  JOURNAL   Arch Oral Biol 160, 105896 (2024)
   PUBMED   38278124
  REMARK    GeneRIF: NOTUM plays a bidirectionally modulatory role in the
            odontoblastic differentiation of human stem cells from the apical
            papilla through the WNT/beta-catenin signaling pathway.
REFERENCE   2  (bases 1 to 2223)
  AUTHORS   Liu,Y., Chen,H., Xiao,L., Dong,P., Ma,Y., Zhou,Y., Yang,J.,
            Bian,B., Xie,G., Chen,L. and Shen,L.
  TITLE     Notum enhances gastric cancer stem-like cell properties through
            upregulation of Sox2 by PI3K/AKT signaling pathway
  JOURNAL   Cell Oncol (Dordr) 47 (2), 463-480 (2024)
   PUBMED   37749430
  REMARK    GeneRIF: Notum enhances gastric cancer stem-like cell properties
            through upregulation of Sox2 by PI3K/AKT signaling pathway.
REFERENCE   3  (bases 1 to 2223)
  AUTHORS   Yang,P., Li,C., Zhou,Q., Zhang,X., Kou,Y., Feng,Q., Wang,H., Su,R.,
            Hasegawa,T., Liu,H. and Li,M.
  TITLE     Notum leads to potential pro-survival of OSCC through crosstalk
            between Shh and Wnt/beta-catenin signaling via p-GSK3beta
  JOURNAL   Int J Biochem Cell Biol 153, 106316 (2022)
   PUBMED   36280040
  REMARK    GeneRIF: Notum leads to potential pro-survival of OSCC through
            crosstalk between Shh and Wnt/beta-catenin signaling via
            p-GSK3beta.
REFERENCE   4  (bases 1 to 2223)
  AUTHORS   Gong,H., Niu,Q., Zhou,Y., Wang,Y.X., Xu,X.F. and Hou,K.Z.
  TITLE     Notum palmitoleoyl-protein carboxylesterase regulates Fas cell
            surface death receptor-mediated apoptosis via the Wnt signaling
            pathway in colon adenocarcinoma
  JOURNAL   Bioengineered 12 (1), 5241-5252 (2021)
   PUBMED   34402722
  REMARK    GeneRIF: Notum palmitoleoyl-protein carboxylesterase regulates Fas
            cell surface death receptor-mediated apoptosis via the Wnt
            signaling pathway in colon adenocarcinoma.
REFERENCE   5  (bases 1 to 2223)
  AUTHORS   Flanagan,D.J., Pentinmikko,N., Luopajarvi,K., Willis,N.J.,
            Gilroy,K., Raven,A.P., Mcgarry,L., Englund,J.I., Webb,A.T.,
            Scharaw,S., Nasreddin,N., Hodder,M.C., Ridgway,R.A., Minnee,E.,
            Sphyris,N., Gilchrist,E., Najumudeen,A.K., Romagnolo,B., Perret,C.,
            Williams,A.C., Clevers,H., Nummela,P., Lahde,M., Alitalo,K.,
            Hietakangas,V., Hedley,A., Clark,W., Nixon,C., Kirschner,K.,
            Jones,E.Y., Ristimaki,A., Leedham,S.J., Fish,P.V., Vincent,J.P.,
            Katajisto,P. and Sansom,O.J.
  TITLE     NOTUM from Apc-mutant cells biases clonal competition to initiate
            cancer
  JOURNAL   Nature 594 (7863), 430-435 (2021)
   PUBMED   34079124
  REMARK    GeneRIF: NOTUM from Apc-mutant cells biases clonal competition to
            initiate cancer.
REFERENCE   6  (bases 1 to 2223)
  AUTHORS   Brommage,R., Liu,J., Vogel,P., Mseeh,F., Thompson,A.Y.,
            Potter,D.G., Shadoan,M.K., Hansen,G.M., Jeter-Jones,S., Cui,J.,
            Bright,D., Bardenhagen,J.P., Doree,D.D., Moverare-Skrtic,S.,
            Nilsson,K.H., Henning,P., Lerner,U.H., Ohlsson,C., Sands,A.T.,
            Tarver,J.E., Powell,D.R., Zambrowicz,B. and Liu,Q.
  TITLE     NOTUM inhibition increases endocortical bone formation and bone
            strength
  JOURNAL   Bone Res 7, 2 (2019)
   PUBMED   30622831
  REMARK    Publication Status: Online-Only
REFERENCE   7  (bases 1 to 2223)
  AUTHORS   Yoon,J.H., Kim,D., Kim,J., Lee,H., Ghim,J., Kang,B.J., Song,P.,
            Suh,P.G., Ryu,S.H. and Lee,T.G.
  TITLE     NOTUM Is Involved in the Progression of Colorectal Cancer
  JOURNAL   Cancer Genomics Proteomics 15 (6), 485-497 (2018)
   PUBMED   30343282
  REMARK    GeneRIF: NOTUM expression was increased in metastatic cells.
            Proliferation was suppressed by inhibiting expression of NOTUM.
            Knockdown of NOTUM genes inhibited proliferation as well as
            migration, with possible involvement of p38 and c-JUN N-terminal
            kinase in this process.
REFERENCE   8  (bases 1 to 2223)
  AUTHORS   De Robertis,M., Arigoni,M., Loiacono,L., Riccardo,F.,
            Calogero,R.A., Feodorova,Y., Tashkova,D., Belovejdov,V.,
            Sarafian,V., Cavallo,F. and Signori,E.
  TITLE     Novel insights into Notum and glypicans regulation in colorectal
            cancer
  JOURNAL   Oncotarget 6 (38), 41237-41257 (2015)
   PUBMED   26517809
  REMARK    GeneRIF: Data show that notum and glypican-1 and glypican-3 gene
            expression during colorectal cancer (CRC) development and present
            evidence to suggest them as potential new biomarkers of CRC
            pathogenesis.
REFERENCE   9  (bases 1 to 2223)
  AUTHORS   Kakugawa,S., Langton,P.F., Zebisch,M., Howell,S., Chang,T.H.,
            Liu,Y., Feizi,T., Bineva,G., O'Reilly,N., Snijders,A.P., Jones,E.Y.
            and Vincent,J.P.
  TITLE     Notum deacylates Wnt proteins to suppress signalling activity
  JOURNAL   Nature 519 (7542), 187-192 (2015)
   PUBMED   25731175
  REMARK    GeneRIF: Kinetic and mass spectrometric analyses of human proteins
            show that Notum is a carboxylesterase that removes an essential
            palmitoleate moiety from Wnt proteins and thus constitutes the
            first known extracellular protein deacylase
REFERENCE   10 (bases 1 to 2223)
  AUTHORS   Torisu,Y., Watanabe,A., Nonaka,A., Midorikawa,Y., Makuuchi,M.,
            Shimamura,T., Sugimura,H., Niida,A., Akiyama,T., Iwanari,H.,
            Kodama,T., Zeniya,M. and Aburatani,H.
  TITLE     Human homolog of NOTUM, overexpressed in hepatocellular carcinoma,
            is regulated transcriptionally by beta-catenin/TCF
  JOURNAL   Cancer Sci 99 (6), 1139-1146 (2008)
   PUBMED   18429952
  REMARK    GeneRIF: Overexpression of NOTUM is associated with hepatocellular
            carcinoma
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AC145207.2, BC060882.1, BX365997.2 and BC036872.1.
            
            On Nov 22, 2018 this sequence version replaced NM_178493.5.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC036872.1, BC060882.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1968189 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000409678.8/ ENSP00000387310.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-6                 AC145207.2         25793-25798
            7-16                BC060882.1         9-18
            17-622              BX365997.2         1-606
            623-2218            BC060882.1         625-2220
            2219-2223           BC036872.1         1762-1766
FEATURES             Location/Qualifiers
     source          1..2223
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q25.3"
     gene            1..2223
                     /gene="NOTUM"
                     /gene_synonym="hNOTUM"
                     /note="notum, palmitoleoyl-protein carboxylesterase"
                     /db_xref="GeneID:147111"
                     /db_xref="HGNC:HGNC:27106"
                     /db_xref="MIM:609847"
     exon            1..601
                     /gene="NOTUM"
                     /gene_synonym="hNOTUM"
                     /inference="alignment:Splign:2.1.0"
     CDS             279..1769
                     /gene="NOTUM"
                     /gene_synonym="hNOTUM"
                     /EC_number="3.1.1.98"
                     /note="protein notum homolog; palmitoleoyl-protein
                     carboxylesterase NOTUM; notum pectinacetylesterase
                     homolog; [Wnt protein] O-palmitoleoyl-L-serine hydrolase"
                     /codon_start=1
                     /product="palmitoleoyl-protein carboxylesterase NOTUM
                     precursor"
                     /protein_id="NP_848588.3"
                     /db_xref="CCDS:CCDS32771.2"
                     /db_xref="GeneID:147111"
                     /db_xref="HGNC:HGNC:27106"
                     /db_xref="MIM:609847"
                     /translation="MGRGVRVLLLLSLLHCAGGSEGRKTWRRRGQQPPPPPRTEAAPA
                     AGQPVESFPLDFTAVEGNMDSFMAQVKSLAQSLYPCSAQQLNEDLRLHLLLNTSVTCN
                     DGSPAGYYLKESRGSRRWLLFLEGGWYCFNRENCDSRYDTMRRLMSSRDWPRTRTGTG
                     ILSSQPEENPYWWNANMVFIPYCSSDVWSGASSKSEKNEYAFMGALIIQEVVRELLGR
                     GLSGAKVLLLAGSSAGGTGVLLNVDRVAEQLEKLGYPAIQVRGLADSGWFLDNKQYRH
                     TDCVDTITCAPTEAIRRGIRYWNGVVPERCRRQFQEGEEWNCFFGYKVYPTLRCPVFV
                     VQWLFDEAQLTVDNVHLTGQPVQEGLRLYIQNLGRELRHTLKDVPASFAPACLSHEII
                     IRSHWTDVQVKGTSLPRALHCWDRSLHDSHKASKTPLKGCPVHLVDSCPWPHCNPSCP
                     TVRDQFTGQEMNVAQFLMHMGFDMQTVAQPQGLEPSELLGMLSNGS"
     exon            602..654
                     /gene="NOTUM"
                     /gene_synonym="hNOTUM"
                     /inference="alignment:Splign:2.1.0"
     exon            655..750
                     /gene="NOTUM"
                     /gene_synonym="hNOTUM"
                     /inference="alignment:Splign:2.1.0"
     exon            751..811
                     /gene="NOTUM"
                     /gene_synonym="hNOTUM"
                     /inference="alignment:Splign:2.1.0"
     exon            812..870
                     /gene="NOTUM"
                     /gene_synonym="hNOTUM"
                     /inference="alignment:Splign:2.1.0"
     exon            871..973
                     /gene="NOTUM"
                     /gene_synonym="hNOTUM"
                     /inference="alignment:Splign:2.1.0"
     exon            974..1165
                     /gene="NOTUM"
                     /gene_synonym="hNOTUM"
                     /inference="alignment:Splign:2.1.0"
     exon            1166..1266
                     /gene="NOTUM"
                     /gene_synonym="hNOTUM"
                     /inference="alignment:Splign:2.1.0"
     exon            1267..1414
                     /gene="NOTUM"
                     /gene_synonym="hNOTUM"
                     /inference="alignment:Splign:2.1.0"
     exon            1415..1462
                     /gene="NOTUM"
                     /gene_synonym="hNOTUM"
                     /inference="alignment:Splign:2.1.0"
     exon            1463..2223
                     /gene="NOTUM"
                     /gene_synonym="hNOTUM"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2223
                     /gene="NOTUM"
                     /gene_synonym="hNOTUM"
                     /note="major polyA site"
ORIGIN      
        1 attggcgcgg gccgcagcca gcgcacccag accctgcgct gccctcggac ggccgggcgc
       61 ggagccccag ctgcggaggc cgacggcacc cggccccgag cgcctcgacg ccgagccgcg
      121 cgcgccttct ccgccaggcc cggcgggcgg gagcgggggc gagggagcag gagcggccag
      181 tgcccccgac acccccggcc cggcaccccc ggcccggcat cccccgccgc cgccgccgcc
      241 gcctcaaggc cgcccgctcc ccgcaggtgg acgcggccat gggccgaggg gtgcgcgtgc
      301 tgctgctgct gagcctgctg cactgcgccg ggggcagcga gggcaggaag acctggcggc
      361 gccggggtca gcagccgcct cctcccccgc ggaccgaggc ggcgccggcg gccggacagc
      421 ccgtggagag cttcccgctg gacttcacgg ccgtggaggg taacatggac agcttcatgg
      481 cgcaagtcaa gagcctggcg cagtccctgt acccctgctc cgcgcagcag ctcaacgagg
      541 acctgcgcct gcacctccta ctcaacacct cggtgacctg caacgacggc agccccgccg
      601 gctactacct gaaggagtcc aggggcagcc ggcggtggct cctcttcctg gaaggcggct
      661 ggtactgctt caaccgcgag aactgcgact ccagatacga caccatgcgg cgcctcatga
      721 gctcccggga ctggccgcgc actcgcacag gcacagggat cctgtcctca cagccggagg
      781 agaaccccta ctggtggaac gcaaacatgg tcttcatccc ctactgctcc agtgatgttt
      841 ggagcggggc ttcatccaag tctgagaaga acgagtacgc cttcatgggc gccctcatca
      901 tccaggaggt ggtgcgggag cttctgggca gagggctgag cggggccaag gtgctgctgc
      961 tggccgggag cagcgcgggg ggcaccgggg tgctcctgaa tgtggaccgt gtggctgagc
     1021 agctggagaa gctgggctac ccagccatcc aggtgcgagg cctggctgac tccggctggt
     1081 tcctggacaa caagcagtat cgccacacag actgcgtcga cacgatcacg tgcgcgccca
     1141 cggaggccat ccgccgtggc atcaggtact ggaacggggt ggtcccggag cgctgccgac
     1201 gccagttcca ggagggcgag gagtggaact gcttctttgg ctacaaggtc tacccgaccc
     1261 tgcgctgccc tgtgttcgtg gtgcagtggc tgtttgacga ggcacagctg acggtggaca
     1321 acgtgcacct gacggggcag ccggtgcagg agggcctgcg gctgtacatc cagaacctcg
     1381 gccgcgagct gcgccacaca ctcaaggacg tgccggccag ctttgccccc gcctgcctct
     1441 cccatgagat catcatccgg agccactgga cggatgtcca ggtgaagggg acgtcgctgc
     1501 cccgagcact gcactgctgg gacaggagcc tccatgacag ccacaaggcc agcaagaccc
     1561 ccctcaaggg ctgccccgtc cacctggtgg acagctgccc ctggccccac tgcaacccct
     1621 catgccccac cgtccgagac cagttcacgg ggcaagagat gaacgtggcc cagttcctca
     1681 tgcacatggg cttcgacatg cagacggtgg cccagccgca gggactggag cccagtgagc
     1741 tgctggggat gctgagcaac ggaagctagg cagactgtct ggaggaggag ccggcactga
     1801 ggggcccaga cacccgctgc cccagtgcca cctcaccccc caccagcagg ccctcccgtc
     1861 tcttcgggac agggccccag ccgtcccccc tgtctgggtc tgcccactgc cctcctgccc
     1921 cggctttccc tgcccctctc ccacagccca gccagagaca agggacctgc tgtcatcccc
     1981 atctgtggcc tgggggtcct tcctgacaac gagggggtag ccagaagaga agcactggat
     2041 tcctcagtcc accagctcag acagcaccca ccggccccac ccatcaagcc cttttatatt
     2101 attttataaa gtgacttttt tattacttta attttttaaa aaaaggaaaa taagaatata
     2161 tgatgaatga tattgttttg taacttttta aaaatgattt taaagagaca aaaaagaacc
     2221 tca
//
