LOCUS       NM_000625               4206 bp    mRNA    linear   PRI 26-FEB-2025
DEFINITION  Homo sapiens nitric oxide synthase 2 (NOS2), mRNA.
ACCESSION   NM_000625
VERSION     NM_000625.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 4206)
  AUTHORS   Astashkin,A.V., Gyawali,Y.P., Jiang,T., Zhang,H. and Feng,C.
  TITLE     Analyzing the FMN-heme interdomain docking interactions in neuronal
            and inducible NOS isoforms by pulsed EPR experiments and
            conformational distribution modeling
  JOURNAL   J Biol Inorg Chem 29 (6), 611-623 (2024)
   PUBMED   39136772
  REMARK    GeneRIF: Analyzing the FMN-heme interdomain docking interactions in
            neuronal and inducible NOS isoforms by pulsed EPR experiments and
            conformational distribution modeling.
REFERENCE   2  (bases 1 to 4206)
  AUTHORS   Sun,Z., Lu,K., He,Q., Tang,Y., Li,H., Pazo,E.E., Hu,L. and Wei,R.
  TITLE     INOS ablation promotes corneal wound healing via activation of Akt
            signaling
  JOURNAL   Exp Eye Res 243, 109886 (2024)
   PUBMED   38583755
  REMARK    GeneRIF: INOS ablation promotes corneal wound healing via
            activation of Akt signaling.
REFERENCE   3  (bases 1 to 4206)
  AUTHORS   Fedele,G., Castiglioni,S., Trapani,V., Zafferri,I., Bartolini,M.,
            Casati,S.M., Ciuffreda,P., Wolf,F.I. and Maier,J.A.
  TITLE     Impact of Inducible Nitric Oxide Synthase Activation on Endothelial
            Behavior under Magnesium Deficiency
  JOURNAL   Nutrients 16 (10), 1406 (2024)
   PUBMED   38794644
  REMARK    GeneRIF: Impact of Inducible Nitric Oxide Synthase Activation on
            Endothelial Behavior under Magnesium Deficiency.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 4206)
  AUTHORS   Wan,W., Zhang,S., Zhao,M., OuYang,X., Yu,Y., Xiong,X., Zhao,N. and
            Jiao,J.
  TITLE     Lysosomal trafficking regulator restricts intracellular growth of
            Coxiella burnetii by inhibiting the expansion of
            Coxiella-containing vacuole and upregulating nos2 expression
  JOURNAL   Front Cell Infect Microbiol 13, 1336600 (2024)
   PUBMED   38282619
  REMARK    GeneRIF: Lysosomal trafficking regulator restricts intracellular
            growth of Coxiella burnetii by inhibiting the expansion of
            Coxiella-containing vacuole and upregulating nos2 expression.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 4206)
  AUTHORS   Bjorner,K., Chen,W.N., Gannavarapu,V.R., Axling,F., Gulyas,M.,
            Halim,M.A., Webb,D.L. and Hellstrom,P.M.
  TITLE     High iNOS and IL-1beta immunoreactivity are features of
            colitis-associated colorectal cancer tumors, but fail to predict
            5-year survival
  JOURNAL   Ups J Med Sci 28, 10241 (2024)
   PUBMED   38187473
  REMARK    GeneRIF: High iNOS and IL-1beta immunoreactivity are features of
            colitis-associated colorectal cancer tumors, but fail to predict
            5-year survival.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 4206)
  AUTHORS   Asano,K., Chee,C.B., Gaston,B., Lilly,C.M., Gerard,C., Drazen,J.M.
            and Stamler,J.S.
  TITLE     Constitutive and inducible nitric oxide synthase gene expression,
            regulation, and activity in human lung epithelial cells
  JOURNAL   Proc Natl Acad Sci U S A 91 (21), 10089-10093 (1994)
   PUBMED   7524082
REFERENCE   7  (bases 1 to 4206)
  AUTHORS   Maier,R., Bilbe,G., Rediske,J. and Lotz,M.
  TITLE     Inducible nitric oxide synthase from human articular chondrocytes:
            cDNA cloning and analysis of mRNA expression
  JOURNAL   Biochim Biophys Acta 1208 (1), 145-150 (1994)
   PUBMED   7522054
REFERENCE   8  (bases 1 to 4206)
  AUTHORS   Chartrain,N.A., Geller,D.A., Koty,P.P., Sitrin,N.F., Nussler,A.K.,
            Hoffman,E.P., Billiar,T.R., Hutchinson,N.I. and Mudgett,J.S.
  TITLE     Molecular cloning, structure, and chromosomal localization of the
            human inducible nitric oxide synthase gene
  JOURNAL   J Biol Chem 269 (9), 6765-6772 (1994)
   PUBMED   7509810
REFERENCE   9  (bases 1 to 4206)
  AUTHORS   Marsden,P.A., Heng,H.H., Duff,C.L., Shi,X.M., Tsui,L.C. and
            Hall,A.V.
  TITLE     Localization of the human gene for inducible nitric oxide synthase
            (NOS2) to chromosome 17q11.2-q12
  JOURNAL   Genomics 19 (1), 183-185 (1994)
   PUBMED   7514565
REFERENCE   10 (bases 1 to 4206)
  AUTHORS   Charles,I.G., Palmer,R.M., Hickery,M.S., Bayliss,M.T., Chubb,A.P.,
            Hall,V.S., Moss,D.W. and Moncada,S.
  TITLE     Cloning, characterization, and expression of a cDNA encoding an
            inducible nitric oxide synthase from the human chondrocyte
  JOURNAL   Proc Natl Acad Sci U S A 90 (23), 11419-11423 (1993)
   PUBMED   7504305
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC130289.11.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Sep 25, 2008 this sequence version replaced NM_000625.3.
            
            Summary: Nitric oxide is a reactive free radical which acts as a
            biologic mediator in several processes, including neurotransmission
            and antimicrobial and antitumoral activities. This gene encodes a
            nitric oxide synthase which is expressed in liver and is inducible
            by a combination of lipopolysaccharide and certain cytokines. Three
            related pseudogenes are located within the Smith-Magenis syndrome
            region on chromosome 17. [provided by RefSeq, Jul 2008].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U31511.1, X73029.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000313735.11/ ENSP00000327251.6
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-191               AC130289.11        107118-107308       c
            192-374             AC130289.11        105479-105661       c
            375-459             AC130289.11        96383-96467         c
            460-582             AC130289.11        95588-95710         c
            583-731             AC130289.11        94457-94605         c
            732-894             AC130289.11        89723-89885         c
            895-986             AC130289.11        88794-88885         c
            987-1128            AC130289.11        87815-87956         c
            1129-1268           AC130289.11        87546-87685         c
            1269-1443           AC130289.11        85661-85835         c
            1444-1545           AC130289.11        85469-85570         c
            1546-1740           AC130289.11        81036-81230         c
            1741-1823           AC130289.11        79940-80022         c
            1824-1968           AC130289.11        79087-79231         c
            1969-2073           AC130289.11        77692-77796         c
            2074-2123           AC130289.11        76314-76363         c
            2124-2298           AC130289.11        75756-75930         c
            2299-2431           AC130289.11        74484-74616         c
            2432-2510           AC130289.11        73289-73367         c
            2511-2692           AC130289.11        72314-72495         c
            2693-2856           AC130289.11        70760-70923         c
            2857-3064           AC130289.11        69577-69784         c
            3065-3152           AC130289.11        67923-68010         c
            3153-3274           AC130289.11        67402-67523         c
            3275-3423           AC130289.11        66809-66957         c
            3424-3618           AC130289.11        65660-65854         c
            3619-4206           AC130289.11        63545-64132         c
FEATURES             Location/Qualifiers
     source          1..4206
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q11.2"
     gene            1..4206
                     /gene="NOS2"
                     /gene_synonym="HEP-NOS; INOS; NOS; NOS2A"
                     /note="nitric oxide synthase 2"
                     /db_xref="GeneID:4843"
                     /db_xref="HGNC:HGNC:7873"
                     /db_xref="MIM:163730"
     exon            1..191
                     /gene="NOS2"
                     /gene_synonym="HEP-NOS; INOS; NOS; NOS2A"
                     /inference="alignment:Splign:2.1.0"
     exon            192..374
                     /gene="NOS2"
                     /gene_synonym="HEP-NOS; INOS; NOS; NOS2A"
                     /inference="alignment:Splign:2.1.0"
     CDS             265..3726
                     /gene="NOS2"
                     /gene_synonym="HEP-NOS; INOS; NOS; NOS2A"
                     /EC_number="1.14.13.39"
                     /note="nitric oxide synthase 2A (inducible, hepatocytes);
                     NOS, type II; nitric oxide synthase, macrophage; inducible
                     NOS; hepatocyte NOS; inducible NO synthase;
                     peptidyl-cysteine S-nitrosylase NOS2; nitric oxide
                     synthase 2, inducible"
                     /codon_start=1
                     /product="nitric oxide synthase, inducible"
                     /protein_id="NP_000616.3"
                     /db_xref="CCDS:CCDS11223.1"
                     /db_xref="GeneID:4843"
                     /db_xref="HGNC:HGNC:7873"
                     /db_xref="MIM:163730"
                     /translation="MACPWKFLFKTKFHQYAMNGEKDINNNVEKAPCATSSPVTQDDL
                     QYHNLSKQQNESPQPLVETGKKSPESLVKLDATPLSSPRHVRIKNWGSGMTFQDTLHH
                     KAKGILTCRSKSCLGSIMTPKSLTRGPRDKPTPPDELLPQAIEFVNQYYGSFKEAKIE
                     EHLARVEAVTKEIETTGTYQLTGDELIFATKQAWRNAPRCIGRIQWSNLQVFDARSCS
                     TAREMFEHICRHVRYSTNNGNIRSAITVFPQRSDGKHDFRVWNAQLIRYAGYQMPDGS
                     IRGDPANVEFTQLCIDLGWKPKYGRFDVVPLVLQANGRDPELFEIPPDLVLEVAMEHP
                     KYEWFRELELKWYALPAVANMLLEVGGLEFPGCPFNGWYMGTEIGVRDFCDVQRYNIL
                     EEVGRRMGLETHKLASLWKDQAVVEINIAVLHSFQKQNVTIMDHHSAAESFMKYMQNE
                     YRSRGGCPADWIWLVPPMSGSITPVFHQEMLNYVLSPFYYYQVEAWKTHVWQDEKRRP
                     KRREIPLKVLVKAVLFACMLMRKTMASRVRVTILFATETGKSEALAWDLGALFSCAFN
                     PKVVCMDKYRLSCLEEERLLLVVTSTFGNGDCPGNGEKLKKSLFMLKELNNKFRYAVF
                     GLGSSMYPRFCAFAHDIDQKLSHLGASQLTPMGEGDELSGQEDAFRSWAVQTFKAACE
                     TFDVRGKQHIQIPKLYTSNVTWDPHHYRLVQDSQPLDLSKALSSMHAKNVFTMRLKSR
                     QNLQSPTSSRATILVELSCEDGQGLNYLPGEHLGVCPGNQPALVQGILERVVDGPTPH
                     QTVRLEALDESGSYWVSDKRLPPCSLSQALTYFLDITTPPTQLLLQKLAQVATEEPER
                     QRLEALCQPSEYSKWKFTNSPTFLEVLEEFPSLRVSAGFLLSQLPILKPRFYSISSSR
                     DHTPTEIHLTVAVVTYHTRDGQGPLHHGVCSTWLNSLKPQDPVPCFVRNASGFHLPED
                     PSHPCILIGPGTGIAPFRSFWQQRLHDSQHKGVRGGRMTLVFGCRRPDEDHIYQEEML
                     EMAQKGVLHAVHTAYSRLPGKPKVYVQDILRQQLASEVLRVLHKEPGHLYVCGDVRMA
                     RDVAHTLKQLVAAKLKLNEEQVEDYFFQLKSQKRYHEDIFGAVFPYEAKKDRVAVQPS
                     SLEMSAL"
     exon            375..459
                     /gene="NOS2"
                     /gene_synonym="HEP-NOS; INOS; NOS; NOS2A"
                     /inference="alignment:Splign:2.1.0"
     exon            460..582
                     /gene="NOS2"
                     /gene_synonym="HEP-NOS; INOS; NOS; NOS2A"
                     /inference="alignment:Splign:2.1.0"
     exon            583..731
                     /gene="NOS2"
                     /gene_synonym="HEP-NOS; INOS; NOS; NOS2A"
                     /inference="alignment:Splign:2.1.0"
     exon            732..894
                     /gene="NOS2"
                     /gene_synonym="HEP-NOS; INOS; NOS; NOS2A"
                     /inference="alignment:Splign:2.1.0"
     exon            895..986
                     /gene="NOS2"
                     /gene_synonym="HEP-NOS; INOS; NOS; NOS2A"
                     /inference="alignment:Splign:2.1.0"
     exon            987..1128
                     /gene="NOS2"
                     /gene_synonym="HEP-NOS; INOS; NOS; NOS2A"
                     /inference="alignment:Splign:2.1.0"
     exon            1129..1268
                     /gene="NOS2"
                     /gene_synonym="HEP-NOS; INOS; NOS; NOS2A"
                     /inference="alignment:Splign:2.1.0"
     exon            1269..1443
                     /gene="NOS2"
                     /gene_synonym="HEP-NOS; INOS; NOS; NOS2A"
                     /inference="alignment:Splign:2.1.0"
     exon            1444..1545
                     /gene="NOS2"
                     /gene_synonym="HEP-NOS; INOS; NOS; NOS2A"
                     /inference="alignment:Splign:2.1.0"
     exon            1546..1740
                     /gene="NOS2"
                     /gene_synonym="HEP-NOS; INOS; NOS; NOS2A"
                     /inference="alignment:Splign:2.1.0"
     exon            1741..1823
                     /gene="NOS2"
                     /gene_synonym="HEP-NOS; INOS; NOS; NOS2A"
                     /inference="alignment:Splign:2.1.0"
     exon            1824..1968
                     /gene="NOS2"
                     /gene_synonym="HEP-NOS; INOS; NOS; NOS2A"
                     /inference="alignment:Splign:2.1.0"
     exon            1969..2073
                     /gene="NOS2"
                     /gene_synonym="HEP-NOS; INOS; NOS; NOS2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2074..2123
                     /gene="NOS2"
                     /gene_synonym="HEP-NOS; INOS; NOS; NOS2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2124..2298
                     /gene="NOS2"
                     /gene_synonym="HEP-NOS; INOS; NOS; NOS2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2299..2431
                     /gene="NOS2"
                     /gene_synonym="HEP-NOS; INOS; NOS; NOS2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2432..2510
                     /gene="NOS2"
                     /gene_synonym="HEP-NOS; INOS; NOS; NOS2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2511..2692
                     /gene="NOS2"
                     /gene_synonym="HEP-NOS; INOS; NOS; NOS2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2693..2856
                     /gene="NOS2"
                     /gene_synonym="HEP-NOS; INOS; NOS; NOS2A"
                     /inference="alignment:Splign:2.1.0"
     exon            2857..3064
                     /gene="NOS2"
                     /gene_synonym="HEP-NOS; INOS; NOS; NOS2A"
                     /inference="alignment:Splign:2.1.0"
     exon            3065..3152
                     /gene="NOS2"
                     /gene_synonym="HEP-NOS; INOS; NOS; NOS2A"
                     /inference="alignment:Splign:2.1.0"
     exon            3153..3274
                     /gene="NOS2"
                     /gene_synonym="HEP-NOS; INOS; NOS; NOS2A"
                     /inference="alignment:Splign:2.1.0"
     exon            3275..3423
                     /gene="NOS2"
                     /gene_synonym="HEP-NOS; INOS; NOS; NOS2A"
                     /inference="alignment:Splign:2.1.0"
     exon            3424..3618
                     /gene="NOS2"
                     /gene_synonym="HEP-NOS; INOS; NOS; NOS2A"
                     /inference="alignment:Splign:2.1.0"
     exon            3619..4206
                     /gene="NOS2"
                     /gene_synonym="HEP-NOS; INOS; NOS; NOS2A"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      4206
                     /gene="NOS2"
                     /gene_synonym="HEP-NOS; INOS; NOS; NOS2A"
                     /note="major polyA site"
ORIGIN      
        1 ataactttgt agcgagtcga aaactgaggc tccggccgca gagaactcag cctcattcct
       61 gctttaaaat ctctcggcca cctttgatga ggggactggg cagttctaga cagtcccgaa
      121 gttctcaagg cacaggtctc ttcctggttt gactgtcctt accccgggga ggcagtgcag
      181 ccagctgcaa gccccacagt gaagaacatc tgagctcaaa tccagataag tgacataagt
      241 gacctgcttt gtaaagccat agagatggcc tgtccttgga aatttctgtt caagaccaaa
      301 ttccaccagt atgcaatgaa tggggaaaaa gacatcaaca acaatgtgga gaaagccccc
      361 tgtgccacct ccagtccagt gacacaggat gaccttcagt atcacaacct cagcaagcag
      421 cagaatgagt ccccgcagcc cctcgtggag acgggaaaga agtctccaga atctctggtc
      481 aagctggatg caaccccatt gtcctcccca cggcatgtga ggatcaaaaa ctggggcagc
      541 gggatgactt tccaagacac acttcaccat aaggccaaag ggattttaac ttgcaggtcc
      601 aaatcttgcc tggggtccat tatgactccc aaaagtttga ccagaggacc cagggacaag
      661 cctacccctc cagatgagct tctacctcaa gctatcgaat ttgtcaacca atattacggc
      721 tccttcaaag aggcaaaaat agaggaacat ctggccaggg tggaagcggt aacaaaggag
      781 atagaaacaa caggaaccta ccaactgacg ggagatgagc tcatcttcgc caccaagcag
      841 gcctggcgca atgccccacg ctgcattggg aggatccagt ggtccaacct gcaggtcttc
      901 gatgcccgca gctgttccac tgcccgggaa atgtttgaac acatctgcag acacgtgcgt
      961 tactccacca acaatggcaa catcaggtcg gccatcaccg tgttccccca gcggagtgat
     1021 ggcaagcacg acttccgggt gtggaatgct cagctcatcc gctatgctgg ctaccagatg
     1081 ccagatggca gcatcagagg ggaccctgcc aacgtggaat tcactcagct gtgcatcgac
     1141 ctgggctgga agcccaagta cggccgcttc gatgtggtcc ccctggtcct gcaggccaat
     1201 ggccgtgacc ctgagctctt cgaaatccca cctgaccttg tgcttgaggt ggccatggaa
     1261 catcccaaat acgagtggtt tcgggaactg gagctaaagt ggtacgccct gcctgcagtg
     1321 gccaacatgc tgcttgaggt gggcggcctg gagttcccag ggtgcccctt caatggctgg
     1381 tacatgggca cagagatcgg agtccgggac ttctgtgacg tccagcgcta caacatcctg
     1441 gaggaagtgg gcaggagaat gggcctggaa acgcacaagc tggcctcgct ctggaaagac
     1501 caggctgtcg ttgagatcaa cattgctgtg ctccatagtt tccagaagca gaatgtgacc
     1561 atcatggacc accactcggc tgcagaatcc ttcatgaagt acatgcagaa tgaataccgg
     1621 tcccgtgggg gctgcccggc agactggatt tggctggtcc ctcccatgtc tgggagcatc
     1681 acccccgtgt ttcaccagga gatgctgaac tacgtcctgt cccctttcta ctactatcag
     1741 gtagaggcct ggaaaaccca tgtctggcag gacgagaagc ggagacccaa gagaagagag
     1801 attccattga aagtcttggt caaagctgtg ctctttgcct gtatgctgat gcgcaagaca
     1861 atggcgtccc gagtcagagt caccatcctc tttgcgacag agacaggaaa atcagaggcg
     1921 ctggcctggg acctgggggc cttattcagc tgtgccttca accccaaggt tgtctgcatg
     1981 gataagtaca ggctgagctg cctggaggag gaacggctgc tgttggtggt gaccagtacg
     2041 tttggcaatg gagactgccc tggcaatgga gagaaactga agaaatcgct cttcatgctg
     2101 aaagagctca acaacaaatt caggtacgct gtgtttggcc tcggctccag catgtaccct
     2161 cggttctgcg cctttgctca tgacattgat cagaagctgt cccacctggg ggcctctcag
     2221 ctcaccccga tgggagaagg ggatgagctc agtgggcagg aggacgcctt ccgcagctgg
     2281 gccgtgcaaa ccttcaaggc agcctgtgag acgtttgatg tccgaggcaa acagcacatt
     2341 cagatcccca agctctacac ctccaatgtg acctgggacc cgcaccacta caggctcgtg
     2401 caggactcac agcctttgga cctcagcaaa gccctcagca gcatgcatgc caagaacgtg
     2461 ttcaccatga ggctcaaatc tcggcagaat ctacaaagtc cgacatccag ccgtgccacc
     2521 atcctggtgg aactctcctg tgaggatggc caaggcctga actacctgcc gggggagcac
     2581 cttggggttt gcccaggcaa ccagccggcc ctggtccaag gtatcctgga gcgagtggtg
     2641 gatggcccca caccccacca gacagtgcgc ctggaggccc tggatgagag tggcagctac
     2701 tgggtcagtg acaagaggct gcccccctgc tcactcagcc aggccctcac ctacttcctg
     2761 gacatcacca cacccccaac ccagctgctg ctccaaaagc tggcccaggt ggccacagaa
     2821 gagcctgaga gacagaggct ggaggccctg tgccagccct cagagtacag caagtggaag
     2881 ttcaccaaca gccccacatt cctggaggtg ctagaggagt tcccgtccct gcgggtgtct
     2941 gctggcttcc tgctttccca gctccccatt ctgaagccca ggttctactc catcagctcc
     3001 tcccgggatc acacgcccac agagatccac ctgactgtgg ccgtggtcac ctaccacacc
     3061 cgagatggcc agggtcccct gcaccacggc gtctgcagca catggctcaa cagcctgaag
     3121 ccccaagacc cagtgccctg ctttgtgcgg aatgccagcg gcttccacct ccccgaggat
     3181 ccctcccatc cttgcatcct catcgggcct ggcacaggca tcgcgccctt ccgcagtttc
     3241 tggcagcaac ggctccatga ctcccagcac aagggagtgc ggggaggccg catgaccttg
     3301 gtgtttgggt gccgccgccc agatgaggac cacatctacc aggaggagat gctggagatg
     3361 gcccagaagg gggtgctgca tgcggtgcac acagcctatt cccgcctgcc tggcaagccc
     3421 aaggtctatg ttcaggacat cctgcggcag cagctggcca gcgaggtgct ccgtgtgctc
     3481 cacaaggagc caggccacct ctatgtttgc ggggatgtgc gcatggcccg ggacgtggcc
     3541 cacaccctga agcagctggt ggctgccaag ctgaaattga atgaggagca ggtcgaggac
     3601 tatttctttc agctcaagag ccagaagcgc tatcacgaag atatctttgg tgctgtattt
     3661 ccttacgagg cgaagaagga cagggtggcg gtgcagccca gcagcctgga gatgtcagcg
     3721 ctctgagggc ctacaggagg ggttaaagct gccggcacag aacttaagga tggagccagc
     3781 tctgcattat ctgaggtcac agggcctggg gagatggagg aaagtgatat cccccagcct
     3841 caagtcttat ttcctcaacg ttgctcccca tcaagccctt tacttgacct cctaacaagt
     3901 agcaccctgg attgatcgga gcctcctctc tcaaactggg gcctccctgg tcccttggag
     3961 acaaaatctt aaatgccagg cctggcaagt gggtgaaaga tggaacttgc tgctgagtgc
     4021 accacttcaa gtgaccacca ggaggtgcta tcgcaccact gtgtatttaa ctgccttgtg
     4081 tacagttatt tatgcctctg tatttaaaaa actaacaccc agtctgttcc ccatggccac
     4141 ttgggtcttc cctgtatgat tccttgatgg agatatttac atgaattgca ttttacttta
     4201 atcaca
//
