LOCUS       NM_000620              12007 bp    mRNA    linear   PRI 11-SEP-2024
DEFINITION  Homo sapiens nitric oxide synthase 1 (NOS1), transcript variant 1,
            mRNA.
ACCESSION   NM_000620
VERSION     NM_000620.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 12007)
  AUTHORS   Nakashima,M., Suga,N., Yoshikawa,S. and Matsuda,S.
  TITLE     Caveolin and NOS in the Development of Muscular Dystrophy
  JOURNAL   Int J Mol Sci 25 (16), 8771 (2024)
   PUBMED   39201459
  REMARK    GeneRIF: Caveolin and NOS in the Development of Muscular Dystrophy.
            Review article
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12007)
  AUTHORS   Kusano,M., Sakai,K., Tomiga,Y., Ito,A., Nakashima,S., Uehara,Y.,
            Kawanaka,K., Kitajima,Y. and Higaki,Y.
  TITLE     The neuronal nitric oxide synthase expression increases during
            satellite cell-derived primary myoblasts differentiation
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 69 (13), 128-133 (2023)
   PUBMED   38158677
  REMARK    GeneRIF: The neuronal nitric oxide synthase expression increases
            during satellite cell-derived primary myoblasts differentiation.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 12007)
  AUTHORS   Liu,R., Juncos,L.A., Lu,Y., Wei,J., Zhang,J., Wang,L., Lai,E.Y.,
            Carlstrom,M. and Persson,A.E.G.
  TITLE     The Role of Macula Densa Nitric Oxide Synthase 1 Beta Splice
            Variant in Modulating Tubuloglomerular Feedback
  JOURNAL   Compr Physiol 13 (1), 4215-4229 (2023)
   PUBMED   36715280
  REMARK    GeneRIF: The Role of Macula Densa Nitric Oxide Synthase 1 Beta
            Splice Variant in Modulating Tubuloglomerular Feedback.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 12007)
  AUTHORS   Boissel,J.P., Zelenka,M., Godtel-Armbrust,U., Feuerstein,T.J. and
            Forstermann,U.
  TITLE     Transcription of different exons 1 of the human neuronal nitric
            oxide synthase gene is dynamically regulated in a cell- and
            stimulus-specific manner
  JOURNAL   Biol Chem 384 (3), 351-362 (2003)
   PUBMED   12715886
REFERENCE   5  (bases 1 to 12007)
  AUTHORS   Larsson,B. and Phillips,S.C.
  TITLE     Isolation and characterization of a novel, human neuronal nitric
            oxide synthase cDNA
  JOURNAL   Biochem Biophys Res Commun 251 (3), 898-902 (1998)
   PUBMED   9791007
REFERENCE   6  (bases 1 to 12007)
  AUTHORS   Wang,Y., Goligorsky,M.S., Lin,M., Wilcox,J.N. and Marsden,P.A.
  TITLE     A novel, testis-specific mRNA transcript encoding an NH2-terminal
            truncated nitric-oxide synthase
  JOURNAL   J Biol Chem 272 (17), 11392-11401 (1997)
   PUBMED   9111048
REFERENCE   7  (bases 1 to 12007)
  AUTHORS   Kishimoto,J., Spurr,N., Liao,M., Lizhi,L., Emson,P. and Xu,W.
  TITLE     Localization of brain nitric oxide synthase (NOS) to human
            chromosome 12
  JOURNAL   Genomics 14 (3), 802-804 (1992)
   PUBMED   1385308
  REMARK    Erratum:[Genomics 1993 Feb;15(2):465]
REFERENCE   8  (bases 1 to 12007)
  AUTHORS   Lowenstein,C.J., Glatt,C.S., Bredt,D.S. and Snyder,S.H.
  TITLE     Cloned and expressed macrophage nitric oxide synthase contrasts
            with the brain enzyme
  JOURNAL   Proc Natl Acad Sci U S A 89 (15), 6711-6715 (1992)
   PUBMED   1379716
REFERENCE   9  (bases 1 to 12007)
  AUTHORS   Bredt,D.S., Ferris,C.D. and Snyder,S.H.
  TITLE     Nitric oxide synthase regulatory sites. Phosphorylation by cyclic
            AMP-dependent protein kinase, protein kinase C, and
            calcium/calmodulin protein kinase; identification of flavin and
            calmodulin binding sites
  JOURNAL   J Biol Chem 267 (16), 10976-10981 (1992)
   PUBMED   1375933
REFERENCE   10 (bases 1 to 12007)
  AUTHORS   Bredt,D.S. and Snyder,S.H.
  TITLE     Isolation of nitric oxide synthetase, a calmodulin-requiring enzyme
  JOURNAL   Proc Natl Acad Sci U S A 87 (2), 682-685 (1990)
   PUBMED   1689048
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from U17327.1, AK294435.1,
            AK307481.1, BC033208.1, AC026364.36 and BE207961.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_000620.4.
            
            Summary: The protein encoded by this gene belongs to the family of
            nitric oxide synthases, which synthesize nitric oxide from
            L-arginine. Nitric oxide is a reactive free radical, which acts as
            a biologic mediator in several processes, including
            neurotransmission, and antimicrobial and antitumoral activities. In
            the brain and peripheral nervous system, nitric oxide displays many
            properties of a neurotransmitter, and has been implicated in
            neurotoxicity associated with stroke and neurodegenerative
            diseases, neural regulation of smooth muscle, including
            peristalsis, and penile erection. This protein is ubiquitously
            expressed, with high level of expression in skeletal muscle.
            Multiple transcript variants that differ in the 5' UTR have been
            described for this gene but the full-length nature of these
            transcripts is not known. Additionally, alternatively spliced
            transcript variants encoding different isoforms (some
            testis-specific) have been found for this gene.[provided by RefSeq,
            Feb 2011].
            
            Transcript Variant: This variant (1) represents the predominant
            transcript, and encodes isoform 1 (also known as nNOS).
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: EU753241.1, U17327.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMN03465402, SAMN03465403
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000317775.11/ ENSP00000320758.6
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-251               U17327.1           152-402
            252-2132            AK294435.1         232-2112
            2133-2736           U17327.1           2283-2886
            2737-4142           AK307481.1         2913-4318
            4143-6577           U17327.1           4293-6727
            6578-7075           BC033208.1         2019-2516
            7076-11757          AC026364.36        33532-38213         c
            11758-12007         BE207961.1         7-256               c
FEATURES             Location/Qualifiers
     source          1..12007
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q24.22"
     gene            1..12007
                     /gene="NOS1"
                     /gene_synonym="bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS"
                     /note="nitric oxide synthase 1"
                     /db_xref="GeneID:4842"
                     /db_xref="HGNC:HGNC:7872"
                     /db_xref="MIM:163731"
     exon            1..115
                     /gene="NOS1"
                     /gene_synonym="bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS"
                     /inference="alignment:Splign:2.1.0"
     exon            116..1260
                     /gene="NOS1"
                     /gene_synonym="bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS"
                     /inference="alignment:Splign:2.1.0"
     CDS             536..4840
                     /gene="NOS1"
                     /gene_synonym="bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS"
                     /EC_number="1.14.13.39"
                     /note="isoform 1 is encoded by transcript variant 1; NOS
                     type I; neuronal NOS; constitutive NOS; peptidyl-cysteine
                     S-nitrosylase NOS1; nitric oxide synthase 1 (neuronal)"
                     /codon_start=1
                     /product="nitric oxide synthase 1 isoform 1"
                     /protein_id="NP_000611.1"
                     /db_xref="CCDS:CCDS41842.1"
                     /db_xref="GeneID:4842"
                     /db_xref="HGNC:HGNC:7872"
                     /db_xref="MIM:163731"
                     /translation="MEDHMFGVQQIQPNVISVRLFKRKVGGLGFLVKERVSKPPVIIS
                     DLIRGGAAEQSGLIQAGDIILAVNGRPLVDLSYDSALEVLRGIASETHVVLILRGPEG
                     FTTHLETTFTGDGTPKTIRVTQPLGPPTKAVDLSHQPPAGKEQPLAVDGASGPGNGPQ
                     HAYDDGQEAGSLPHANGLAPRPPGQDPAKKATRVSLQGRGENNELLKEIEPVLSLLTS
                     GSRGVKGGAPAKAEMKDMGIQVDRDLDGKSHKPLPLGVENDRVFNDLWGKGNVPVVLN
                     NPYSEKEQPPTSGKQSPTKNGSPSKCPRFLKVKNWETEVVLTDTLHLKSTLETGCTEY
                     ICMGSIMHPSQHARRPEDVRTKGQLFPLAKEFIDQYYSSIKRFGSKAHMERLEEVNKE
                     IDTTSTYQLKDTELIYGAKHAWRNASRCVGRIQWSKLQVFDARDCTTAHGMFNYICNH
                     VKYATNKGNLRSAITIFPQRTDGKHDFRVWNSQLIRYAGYKQPDGSTLGDPANVQFTE
                     ICIQQGWKPPRGRFDVLPLLLQANGNDPELFQIPPELVLEVPIRHPKFEWFKDLGLKW
                     YGLPAVSNMLLEIGGLEFSACPFSGWYMGTEIGVRDYCDNSRYNILEEVAKKMNLDMR
                     KTSSLWKDQALVEINIAVLYSFQSDKVTIVDHHSATESFIKHMENEYRCRGGCPADWV
                     WIVPPMSGSITPVFHQEMLNYRLTPSFEYQPDPWNTHVWKGTNGTPTKRRAIGFKKLA
                     EAVKFSAKLMGQAMAKRVKATILYATETGKSQAYAKTLCEIFKHAFDAKVMSMEEYDI
                     VHLEHETLVLVVTSTFGNGDPPENGEKFGCALMEMRHPNSVQEERKSYKVRFNSVSSY
                     SDSQKSSGDGPDLRDNFESAGPLANVRFSVFGLGSRAYPHFCAFGHAVDTLLEELGGE
                     RILKMREGDELCGQEEAFRTWAKKVFKAACDVFCVGDDVNIEKANNSLISNDRSWKRN
                     KFRLTFVAEAPELTQGLSNVHKKRVSAARLLSRQNLQSPKSSRSTIFVRLHTNGSQEL
                     QYQPGDHLGVFPGNHEDLVNALIERLEDAPPVNQMVKVELLEERNTALGVISNWTDEL
                     RLPPCTIFQAFKYYLDITTPPTPLQLQQFASLATSEKEKQRLLVLSKGLQEYEEWKWG
                     KNPTIVEVLEEFPSIQMPATLLLTQLSLLQPRYYSISSSPDMYPDEVHLTVAIVSYRT
                     RDGEGPIHHGVCSSWLNRIQADELVPCFVRGAPSFHLPRNPQVPCILVGPGTGIAPFR
                     SFWQQRQFDIQHKGMNPCPMVLVFGCRQSKIDHIYREETLQAKNKGVFRELYTAYSRE
                     PDKPKKYVQDILQEQLAESVYRALKEQGGHIYVCGDVTMAADVLKAIQRIMTQQGKLS
                     AEDAGVFISRMRDDNRYHEDIFGVTLRTYEVTNRLRSESIAFIEESKKDTDEVFSS"
     exon            1261..1387
                     /gene="NOS1"
                     /gene_synonym="bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS"
                     /inference="alignment:Splign:2.1.0"
     exon            1388..1516
                     /gene="NOS1"
                     /gene_synonym="bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS"
                     /inference="alignment:Splign:2.1.0"
     exon            1517..1662
                     /gene="NOS1"
                     /gene_synonym="bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS"
                     /inference="alignment:Splign:2.1.0"
     exon            1663..1825
                     /gene="NOS1"
                     /gene_synonym="bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS"
                     /inference="alignment:Splign:2.1.0"
     exon            1826..1917
                     /gene="NOS1"
                     /gene_synonym="bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS"
                     /inference="alignment:Splign:2.1.0"
     exon            1918..2059
                     /gene="NOS1"
                     /gene_synonym="bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS"
                     /inference="alignment:Splign:2.1.0"
     exon            2060..2199
                     /gene="NOS1"
                     /gene_synonym="bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS"
                     /inference="alignment:Splign:2.1.0"
     exon            2200..2374
                     /gene="NOS1"
                     /gene_synonym="bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS"
                     /inference="alignment:Splign:2.1.0"
     exon            2375..2476
                     /gene="NOS1"
                     /gene_synonym="bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS"
                     /inference="alignment:Splign:2.1.0"
     exon            2477..2671
                     /gene="NOS1"
                     /gene_synonym="bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS"
                     /inference="alignment:Splign:2.1.0"
     exon            2672..2757
                     /gene="NOS1"
                     /gene_synonym="bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS"
                     /inference="alignment:Splign:2.1.0"
     exon            2758..2902
                     /gene="NOS1"
                     /gene_synonym="bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS"
                     /inference="alignment:Splign:2.1.0"
     exon            2903..3007
                     /gene="NOS1"
                     /gene_synonym="bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS"
                     /inference="alignment:Splign:2.1.0"
     exon            3008..3066
                     /gene="NOS1"
                     /gene_synonym="bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS"
                     /inference="alignment:Splign:2.1.0"
     exon            3067..3183
                     /gene="NOS1"
                     /gene_synonym="bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS"
                     /inference="alignment:Splign:2.1.0"
     exon            3184..3358
                     /gene="NOS1"
                     /gene_synonym="bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS"
                     /inference="alignment:Splign:2.1.0"
     exon            3359..3497
                     /gene="NOS1"
                     /gene_synonym="bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS"
                     /inference="alignment:Splign:2.1.0"
     exon            3498..3576
                     /gene="NOS1"
                     /gene_synonym="bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS"
                     /inference="alignment:Splign:2.1.0"
     exon            3577..3770
                     /gene="NOS1"
                     /gene_synonym="bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS"
                     /inference="alignment:Splign:2.1.0"
     exon            3771..3940
                     /gene="NOS1"
                     /gene_synonym="bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS"
                     /inference="alignment:Splign:2.1.0"
     exon            3941..4151
                     /gene="NOS1"
                     /gene_synonym="bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS"
                     /inference="alignment:Splign:2.1.0"
     exon            4152..4239
                     /gene="NOS1"
                     /gene_synonym="bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS"
                     /inference="alignment:Splign:2.1.0"
     exon            4240..4361
                     /gene="NOS1"
                     /gene_synonym="bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS"
                     /inference="alignment:Splign:2.1.0"
     exon            4362..4510
                     /gene="NOS1"
                     /gene_synonym="bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS"
                     /inference="alignment:Splign:2.1.0"
     exon            4511..4705
                     /gene="NOS1"
                     /gene_synonym="bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS"
                     /inference="alignment:Splign:2.1.0"
     exon            4706..4824
                     /gene="NOS1"
                     /gene_synonym="bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS"
                     /inference="alignment:Splign:2.1.0"
     exon            4825..12007
                     /gene="NOS1"
                     /gene_synonym="bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      11990..11995
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NOS1"
                     /gene_synonym="bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS"
                     /note="hexamer: ATTAAA"
     polyA_site      12007
                     /gene="NOS1"
                     /gene_synonym="bNOS; IHPS1; N-NOS; NC-NOS; nNOS; NOS"
                     /note="major polyA site"
ORIGIN      
        1 acggctcagt ccctacatat ttatgccgcg tttccagccg ctgggtgagg agctacttag
       61 cgccgcggct cctccgaggg gcggccgggc agcgagcagc ggccgagcgg acgggctcat
      121 gatgcctcag atctgatccg catctaacag gctggcaatg aagataccca gagaatagtt
      181 cacatctatc atgcgtcact tctagacaca gccatcagac gcatctcctc ccctttctgc
      241 ctgaccttag ggacacgtcc caccgcctct cttgacgtct gcctggtcaa ccatcacttc
      301 cttagagaat aaggagagag gcggatgcag gaaatcatgc caccgacggg ccaccagcca
      361 tgagtgggtg acgctgagct gacgtcaaag acagagaggg ctgaagcctt gtcagcacct
      421 gtcaccccgg ctcctgctct ccgtgtagcc tgaagcctgg atcctcctgg tgaaatcatc
      481 ttggcctgat agcattgtga ggtcttcaga caggacccct cggaagctag ttaccatgga
      541 ggatcacatg ttcggtgttc agcaaatcca gcccaatgtc atttctgttc gtctcttcaa
      601 gcgcaaagtt gggggcctgg gatttctggt gaaggagcgg gtcagtaagc cgcccgtgat
      661 catctctgac ctgattcgtg ggggcgccgc agagcagagt ggcctcatcc aggccggaga
      721 catcattctt gcggtcaacg gccggccctt ggtggacctg agctatgaca gcgccctgga
      781 ggtactcaga ggcattgcct ctgagaccca cgtggtcctc attctgaggg gccctgaagg
      841 tttcaccacg cacctggaga ccacctttac aggtgatggg acccccaaga ccatccgggt
      901 gacacagccc ctgggtcccc ccaccaaagc cgtggatctg tcccaccagc caccggccgg
      961 caaagaacag cccctggcag tggatggggc ctcgggtccc gggaatgggc ctcagcatgc
     1021 ctacgatgat gggcaggagg ctggctcact cccccatgcc aacggcctgg cccccaggcc
     1081 cccaggccag gaccccgcga agaaagcaac cagagtcagc ctccaaggca gaggggagaa
     1141 caatgaactg ctcaaggaga tagagcctgt gctgagcctt ctcaccagtg ggagcagagg
     1201 ggtcaaggga ggggcacctg ccaaggcaga gatgaaagat atgggaatcc aggtggacag
     1261 agatttggac ggcaagtcac acaaacctct gcccctcggc gtggagaacg accgagtctt
     1321 caatgaccta tgggggaagg gcaatgtgcc tgtcgtcctc aacaacccat attcagagaa
     1381 ggagcagccc cccacctcag gaaaacagtc ccccacaaag aatggcagcc cctccaagtg
     1441 tccacgcttc ctcaaggtca agaactggga gactgaggtg gttctcactg acaccctcca
     1501 ccttaagagc acattggaaa cgggatgcac tgagtacatc tgcatgggct ccatcatgca
     1561 tccttctcag catgcaagga ggcctgaaga cgtccgcaca aaaggacagc tcttccctct
     1621 cgccaaagag tttattgatc aatactattc atcaattaaa agatttggct ccaaagccca
     1681 catggaaagg ctggaagagg tgaacaaaga gatcgacacc actagcactt accagctcaa
     1741 ggacacagag ctcatctatg gggccaagca cgcctggcgg aatgcctcgc gctgtgtggg
     1801 caggatccag tggtccaagc tgcaggtatt cgatgcccgt gactgcacca cggcccacgg
     1861 gatgttcaac tacatctgta accatgtcaa gtatgccacc aacaaaggga acctcaggtc
     1921 tgccatcacc atattccccc agaggacaga cggcaagcac gacttccgag tctggaactc
     1981 ccagctcatc cgctacgctg gctacaagca gcctgacggc tccaccctgg gggacccagc
     2041 caatgtgcag ttcacagaga tatgcataca gcagggctgg aaaccgccta gaggccgctt
     2101 cgatgtcctg ccgctcctgc ttcaggccaa cggcaatgac cctgagctct tccagattcc
     2161 tccagagctg gtgttggaag ttcccatcag gcaccccaag tttgagtggt tcaaggacct
     2221 ggggctgaag tggtacggcc tccccgccgt gtccaacatg ctcctagaga ttggcggcct
     2281 ggagttcagc gcctgtccct tcagtggctg gtacatgggc acagagattg gtgtccgcga
     2341 ctactgtgac aactcccgct acaatatcct ggaggaagtg gccaagaaga tgaacttaga
     2401 catgaggaag acgtcctccc tgtggaagga ccaggcgctg gtggagatca atatcgcggt
     2461 tctctatagc ttccagagtg acaaagtgac cattgttgac catcactccg ccaccgagtc
     2521 cttcattaag cacatggaga atgagtaccg ctgccggggg ggctgccctg ccgactgggt
     2581 gtggatcgtg ccccccatgt ccggaagcat cacccctgtg ttccaccagg agatgctcaa
     2641 ctaccggctc accccctcct tcgaatacca gcctgatccc tggaacacgc atgtctggaa
     2701 aggcaccaac gggaccccca caaagcggcg agccattggc ttcaagaagc tagcagaagc
     2761 tgtcaagttc tcggccaagc tgatggggca ggctatggcc aagagggtga aagcgaccat
     2821 cctctatgcc acagagacag gcaaatcgca agcttatgcc aagaccttgt gtgagatctt
     2881 caaacacgcc tttgatgcca aggtgatgtc catggaagaa tatgacattg tgcacctgga
     2941 acatgaaact ctggtccttg tggtcaccag cacctttggc aatggagatc cccctgagaa
     3001 tggggagaaa ttcggctgtg ctttgatgga aatgaggcac cccaactctg tgcaggaaga
     3061 aaggaagagc tacaaggtcc gattcaacag cgtctcctcc tactctgact cccaaaaatc
     3121 atcaggcgat gggcccgacc tcagagacaa ctttgagagt gctggacccc tggccaatgt
     3181 gaggttctca gtttttggcc tcggctcacg agcataccct cacttttgcg ccttcggaca
     3241 cgctgtggac accctcctgg aagaactggg aggggagagg atcctgaaga tgagggaagg
     3301 ggatgagctc tgtgggcagg aagaggcttt caggacctgg gccaagaagg tcttcaaggc
     3361 agcctgtgat gtcttctgtg tgggagatga tgtcaacatt gaaaaggcca acaattccct
     3421 catcagcaat gatcgcagct ggaagagaaa caagttccgc ctcacctttg tggccgaagc
     3481 tccagaactc acacaaggtc tatccaatgt ccacaaaaag cgagtctcag ctgcccggct
     3541 ccttagccgt caaaacctcc agagccctaa atccagtcgg tcaactatct tcgtgcgtct
     3601 ccacaccaac gggagccagg agctgcagta ccagcctggg gaccacctgg gtgtcttccc
     3661 tggcaaccac gaggacctcg tgaatgccct gatcgagcgg ctggaggacg cgccgcctgt
     3721 caaccagatg gtgaaagtgg aactgctgga ggagcggaac acggctttag gtgtcatcag
     3781 taactggaca gacgagctcc gcctcccgcc ctgcaccatc ttccaggcct tcaagtacta
     3841 cctggacatc accacgccac caacgcctct gcagctgcag cagtttgcct ccctagctac
     3901 cagcgagaag gagaagcagc gtctgctggt cctcagcaag ggtttgcagg agtacgagga
     3961 atggaaatgg ggcaagaacc ccaccatcgt ggaggtgctg gaggagttcc catctatcca
     4021 gatgccggcc accctgctcc tgacccagct gtccctgctg cagccccgct actattccat
     4081 cagctcctcc ccagacatgt accctgatga agtgcacctc actgtggcca tcgtttccta
     4141 ccgcactcga gatggagaag gaccaattca ccacggcgta tgctcctcct ggctcaaccg
     4201 gatacaggct gacgaactgg tcccctgttt cgtgagagga gcacccagct tccacctgcc
     4261 ccggaacccc caagtcccct gcatcctcgt tggaccaggc accggcattg cccctttccg
     4321 aagcttctgg caacagcggc aatttgatat ccaacacaaa ggaatgaacc cctgccccat
     4381 ggtcctggtc ttcgggtgcc ggcaatccaa gatagatcat atctacaggg aagagaccct
     4441 gcaggccaag aacaaggggg tcttcagaga gctgtacacg gcttactccc gggagccaga
     4501 caaaccaaag aagtacgtgc aggacatcct gcaggagcag ctggcggagt ctgtgtaccg
     4561 agccctgaag gagcaagggg gccacatata cgtctgtggg gacgtcacca tggctgctga
     4621 tgtcctcaaa gccatccagc gcatcatgac ccagcagggg aagctctcgg cagaggacgc
     4681 cggcgtattc atcagccgga tgagggatga caaccgatac catgaggata tttttggagt
     4741 caccctgcga acgtacgaag tgaccaaccg ccttagatct gagtccattg ccttcattga
     4801 agagagcaaa aaagacaccg atgaggtttt cagctcctaa ctggaccctc ttgcccagcc
     4861 ggctgcaagt tttgtaagcg cggacagaca ctgctgaacc tttcctctgg gaccccctgt
     4921 ggccctcgct ctgcctcctg tccttgtcgc tgtgccctgg tttccctcct cgggcttctc
     4981 gcccctcagt ggtttcctcg gccctcctgg gtttactcct tgagttttcc tgctgcgatg
     5041 caatgctttt ctaatctgca gtggctctta caaaactctg ttcccactcc ctctcttgcc
     5101 gacaagggca actcacgggt gcatgaaacc actggaacat ggccgtcgct gtgggggttt
     5161 ttttctctgg ggttcccctg gaaaggctgc aggaactagg cacaagctct ctgagccagt
     5221 ccctcagcca ctgaagtccc cctttctcct tttttatgat gacattttgg ttgtgcgtgc
     5281 ctgtgtgtgt gtgtgtgtgt gtgtgtgtgt gtgtgatggg ccaggtctct gtccgtcctc
     5341 ttccctgcac aagtgtgtcg atcttagatt gccactgctt tcattgaaga ccctcaatgc
     5401 caagaaacgt gtccctggcc catattaatc cctcgtgtgt ccataattag ggtccacgcc
     5461 catgtacctg aaacatttgg aagccccata attgttctag ttagaaaggg ttcagggcat
     5521 ggggagagga gtgggaaatt gattaaaggg gctgtctccc aatgaaagag gcattcccag
     5581 aatttgctgc atttagattt tgataccagt gagcagagcc ctcatgtgac atgaacccat
     5641 ccaatggatt gtgcaaatcc cctccccaaa cccacccata ccagctagaa tcacttgact
     5701 ttgccacatc cattgactga ccccctcctc cagcaatagc atccaagggg cctggaagtt
     5761 atgttgttca aagaagcctg gtggcaataa ggatcttccc actttgccac tggatgactt
     5821 tggatgggtc acttgtcctc agtttttcct agtcataatg tcatacgaac ctaaagaata
     5881 tgaatggatt aaatgttaaa gctttggtgc ctggaaacaa tatcaagtaa caatatgatt
     5941 attatttttt tattccccca aagcgggctt gctgcttcac ccttggggat gaaataatgg
     6001 aagctggtta aagtggatga ggttggaaag agttgccata atgaggtccc acgtggcttc
     6061 ttcgatagga gccacaactt ggggtgggaa gaacttgtcc ctcaggcttg ttgccctctg
     6121 cagttgatct ccaaagtttt aaacctgtta aattaatttt gacaaataag ttaccctcaa
     6181 ctcagatcaa aaatgggcag ccaagtcttc ggtaggaatt ggagccggtg taattcctcc
     6241 ctaagaggca acctgttgaa tttactctct cagagtaaat ggtgggaagg gatccctttg
     6301 tatacttttt taaatactac aaattagtgt caggcagttc ccagaaagag acaagaaatc
     6361 ctagtggcct cccagactgc agggtcccca aggatggaaa gggaatgttc tgctggttct
     6421 accctgtttg ttgtgtcttg ctatacagaa aaaccacatt tcttttatat actgtacgtg
     6481 ggcatatctt gttgttcagt ttgggtgtct gctaaagagg aagtgcactg gccctctttg
     6541 aaagggcttt acagtggggg caccaagacc ccaaagggcc caggccagga gactgttaaa
     6601 gtgaaaaggc aatctatgac tcaccttgct ctgccatccc tggcagcccc caccggtgtc
     6661 ctgttcctgc cacatggagc ttgacttcat gccagctata atctcccctg ccttccttta
     6721 atcccaattt cccctgctca ctcttccaca gatataaaga acaaacactt agcatcccac
     6781 actcacccct tctaatcctg aagggaagcc cattctaaac tcctttcctg caaacccatt
     6841 tccagctcct agtagctttc ctcccaaagg ctttctttcc aatcctttat agctttggag
     6901 acgcctcccc aattccccag ggaaggaaac tgttgtgtcc aatccccatt aaagacaaat
     6961 tgatcagtgc ttcccactcc aagtcaagct ttatgcagga atgcttttcc atcagggaat
     7021 aaatacttag aagcgcttac aaggtgccag gcacctcctt tctgcatgtg cctgcctttc
     7081 tagtagcaga cagatggaaa cattgtctca ttttgtcaag gagtccaaag aaatgattat
     7141 aaaaccagga ttcatccttc ttctccagaa agattttttt ttaagtaaac acctttcaat
     7201 ccccaacaca agctgcttca caactccagg ctagaaggca ggagagcgat ctgatgtgtt
     7261 tctttcattt gccagaattc ctgataccaa aagcctctct ctctgttgag taacctctca
     7321 aggaccagag tggagtccag attgttaggc tcagatcaag ggtggggaaa tactgccctc
     7381 tcgtggtggc ttttcatcca ggcctcgtag ccaaccgttt aagtgcaaaa tagaattaag
     7441 caatgggtaa gcaaaatagg gttgacaaga tatttggggg ttattcgggt tatggcccat
     7501 ttatttccct cttccccctg aattgaccag tagcagctcc agccccattt cacaaaagtg
     7561 agtttggcca ggaggaatga gacgtctcct gaaataggaa caccggaaca tcatgctcac
     7621 ctgccatcac tatgcatcca gttcccacag cttgtgtcgt gaaagagcag agagatgatg
     7681 ttaaactcct tgggaggaga gagggcttct tttggtttcc ctggagtgag acagccaggt
     7741 gtctttcttt tgcgggggga cacttcagac ccatcaatat ggaattttgg gagccgacct
     7801 gagtgcaaat cctaattctg cccctgttgg tgcagatggc tgtgggcggc tcacttgacc
     7861 ttttagagtc tgcataccca cctgtataac aaggtggatt gaatgagaca atgcccacga
     7921 aatgcccagt tacagtacct ggttcaaaac ttactgcatt ttaatttttc acttaactta
     7981 taacatgtct tgcttctcca gtgtgtggaa ggcaccgggc agtttgcaga gataagcaaa
     8041 acacagttcc tctcgtgcag aaggttagaa tctatttttt tttttgacag agtcttgctc
     8101 tgtcacccag gctggcgtac agtggtacga tctcagctca ctgcatcctc tgcctccccc
     8161 agttcaagtg attcttctgc ctcggcctcc tgagtaactg ggactacagg cgcctaccac
     8221 cacgcccagc taagttttgt atttttagta gagtcagggt ttcaccatgt tggccaggct
     8281 ggtcttgaat tcctgacctc aaatgatcca cgcacctcag cctcccaaag tgctggatta
     8341 caggcatgag ccaccacgcc cagccaaagg ttataatctg atggagagag acacccgtct
     8401 tggaactgac ataaatttct ggggtttgag aaatgggcgg gatttcactg gtagcttctg
     8461 gaaggtaaga gttgtccagg aattgggaag agtgagagga aaggcacgga cagggagcat
     8521 gtaagataaa ttgaggctgg ctttggaagg ctgaggaggg tgagaaaagg tgggctggga
     8581 ccagaccgtg gggagaggtg agtggcatta caagaaattt aggctttatt cagaaggcaa
     8641 cagggagtcc ctaagaatgt ttttcaaaaa gggacattaa ggcgattgga gttatacttg
     8701 gaaaagaaag ttctggccac agtacagagc atggcccgtt gagctgttgg gggggttatt
     8761 gctgcaacca aggcttgagt gagggaagag gcggatgtag tgataaagag actccaggaa
     8821 ctgaatcagc gtacctggca ccccatccat tgtagagggt gagaataaag gagaaattaa
     8881 agcatcttgc aggctgggcg cggtagctca tgtctgtaat cccagcactt tgggaggccg
     8941 aggtgggtgt atcagttgag gtcaggagtt ggagaccagt cagccagtta gtagaaaccc
     9001 tgactctact aagaaaatac aaaaattagc tgggcatggt ggcatgcgcc tgtagtctca
     9061 gctacctggg aggctgagga aggaggatcg cttgagccca ggaggtggag gctgcagtga
     9121 gccaagattg taccactgca ctccagcctg ggtgacagag caagactctt atctcaaaaa
     9181 aaataaaata aaataaaata aaataaaaca tcttgcccct agctgagaga gaggtctctg
     9241 aagagcaggc tcagggaaaa gatgagtttt cagagctgat gtgatagtca gcttctctgg
     9301 agtcaacagg gtgaatcctt cccaagtcca gccatgccca gatgcccgga gggaaaactg
     9361 acccccagcc agtagacatt ggctaagaac acagaatctt ctgaccaaac acgctttcag
     9421 cagctgcctg ctctggactt tgaaagaggt caggtcttgc cctaagctca aaacaagtga
     9481 gaggtgtcct gacctagctc atagggcaaa tggtcctaat aggatgggca atccagatgc
     9541 ctgagcccct tcactccgac agcaccagcg cctaatgcag ccttttcatt cttgccatta
     9601 ggaaatctgt ggacttctag cctgtgtttt aaaccagcca tgtttccttg tatatttccc
     9661 tacccgctgc cccacatacc cagcatgccg ctgtggccac catgtcctca aagccttctg
     9721 tctgtatcag gaatgtagtc tgagactgcc aggaagcaac aaggagagag aaacactaac
     9781 tagtcttcct ttataaccca ttcatactct ctggctgtcc ccaaccttca tagtctcctg
     9841 catccaaatg tcctctttgg ctcaaaaagt aggccaggca tggtggttca tgcctgtaat
     9901 agcactttgg gagactgagg tgggaggatc acttggggcc aggagtttga gaccagcttg
     9961 ggcaacacag cgcaatctcg tctctactaa aaaaaaaaaa aaaaaaaaat tagctgggca
    10021 tgatggcatg ctcctgtggt cccagctact tgggaggctg aggcaggagg atcacttggt
    10081 cccaggagtt tgaggcgaca gtgagctagg atcgcaccac tgcactccag cctgagtgac
    10141 agagcaagac cctgtctcta aaaaaaatta aaatgaaaga ccaggtgctg ggattaagga
    10201 aacacaggtc tgagggtctg agggaagggg cctgcctccc agggagtcaa catagatgtt
    10261 ccccatgaac agggatttga ctttggaggc caacctggcc tggcctctgc cctttatctc
    10321 acactcccta tccttggccc actgccagtc cctgccttgt ggcaaagggg ccccaaaaga
    10381 aaagctgccc ttccccaaat gtaaggaccc aggtacactt tcacccgtgg aaagcagtgt
    10441 ctgtcgagag tctgtttcct attaatactt atcaaagcca tgtgcgaggg aggtggtcag
    10501 ctgtcaatat gccttagtat gtttatatga gtttgttttg ttctaaaata cccaaacagt
    10561 tctggtcaag cggggctatg cccgtctggc ccaaaacaca gtccgttatt aacgagatgg
    10621 ccctggcagg cgggaacaaa tctgcctcca tgcactgctt cctgtagtct tttagaaagt
    10681 aactccagga catcgaagtg cccagatttg actcctaagt tctaggagac tgtagcgcag
    10741 ggtctgtcaa ccttagcact attggcattt ggggctgggt aattctttct tgtgggggcc
    10801 gtcttgggta ctgtaggaag ctgagcagca ttcctggcct ccatccacaa gatacctgta
    10861 gcagtgtcct gccaacggta acaatcaagt atgtcatcag acattgccca atgtccccag
    10921 ggggcaacac ccctctcttg gacttcaggg tcaagagaat ctctgctggc taccccagga
    10981 cttctcatta tagatttcct ggagcacgca gcagaaactt tgcctagccc agtggttgtt
    11041 tccattatct gctgccaaag tgggatttga gggtgtccgg gggagggggc atggggaggg
    11101 cagtatgctt tcaaaaaccc ctcccaggcc aggcgtggtg gctcatgcct gtaatcacag
    11161 gactttggga ggccgaggct ggcagatcac ttgaggctgg gagttagaga ccaacctggc
    11221 taacatggca aaacctcgtc tctactaaaa atacaaaaat cagcccggcg tggtggcggg
    11281 catctgtaat cccatctact cgggaggctg aggcaggaga attacttgaa cccaggaggc
    11341 agaggctgca gtgagccgag atggcaccac tgcactccag cttgttgaca gaatgagacc
    11401 ctgtggaaaa aaaaaaaaaa gccctcccat gccagaacag aggatggcag tctgtttcaa
    11461 taagacactg tgtccttggt gttggttctg attaagactc actgagatcc agtgctcttg
    11521 agctgggtct cagtcccctc ccatgtcctg tgctctgccg ccactgtttt cattgttgtg
    11581 ttctcgttgt gattgttaag actcacactc ctggctcagc agtggttttc cagaaggccc
    11641 aaagagcggt gccgggcacc ccacgtcgca gtgtccgttc cgggcttggg aagctgggga
    11701 ggtgggcaga cctggtcgca tctcaccaca cacacacaca cacacacaca cacacacgct
    11761 gtcagaaact cggccgtccc ccctacctct gagctctcaa tgctgctaat ctctgccaag
    11821 tgtccctgtg ctccagcacc ttccttgaag gactgacgcc caccccacgc tctttgcgag
    11881 gttgtccagg ctgtgtttgt cgcatgctct tcttctgtat agttctcatc ttccaatttt
    11941 atgggattca acaaaagcct attatgcttg tttgcattat ggttacaata ttaaaaagtg
    12001 gattcaa
//
