LOCUS       NM_001258308            2650 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens NOP2 nucleolar protein (NOP2), transcript variant 3,
            mRNA.
ACCESSION   NM_001258308
VERSION     NM_001258308.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2650)
  AUTHORS   Yang,Y., Fan,H., Liu,H., Lou,X., Xiao,N., Zhang,C., Chen,H.,
            Chen,S., Gu,H., Liu,H. and Wan,J.
  TITLE     NOP2 facilitates EZH2-mediated epithelial-mesenchymal transition by
            enhancing EZH2 mRNA stability via m5C methylation in lung cancer
            progression
  JOURNAL   Cell Death Dis 15 (7), 506 (2024)
   PUBMED   39013911
  REMARK    GeneRIF: NOP2 facilitates EZH2-mediated epithelial-mesenchymal
            transition by enhancing EZH2 mRNA stability via m5C methylation in
            lung cancer progression.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2650)
  AUTHORS   Sun,G.F. and Ding,H.
  TITLE     NOP2-mediated m5C methylation of XPD is associated with
            hepatocellular carcinoma progression
  JOURNAL   Neoplasma 70 (3), 340-349 (2023)
   PUBMED   37498063
  REMARK    GeneRIF: NOP2-mediated m5C methylation of XPD is associated with
            hepatocellular carcinoma progression.
REFERENCE   3  (bases 1 to 2650)
  AUTHORS   Liao,H., Gaur,A., McConie,H., Shekar,A., Wang,K., Chang,J.T.,
            Breton,G. and Denicourt,C.
  TITLE     Human NOP2/NSUN1 regulates ribosome biogenesis through
            non-catalytic complex formation with box C/D snoRNPs
  JOURNAL   Nucleic Acids Res 50 (18), 10695-10716 (2022)
   PUBMED   36161484
  REMARK    GeneRIF: Human NOP2/NSUN1 regulates ribosome biogenesis through
            non-catalytic complex formation with box C/D snoRNPs.
REFERENCE   4  (bases 1 to 2650)
  AUTHORS   Feng,J., Zhang,J., Li,Y., Wang,J., Mo,P. and Lin,L.
  TITLE     Upregulated expression of NOP2 predicts worse prognosis of gastric
            adenocarcinoma by promoting tumor growth
  JOURNAL   Saudi J Gastroenterol 28 (5), 369-377 (2022)
   PUBMED   35381832
  REMARK    GeneRIF: Upregulated expression of NOP2 predicts worse prognosis of
            gastric adenocarcinoma by promoting tumor growth.
REFERENCE   5  (bases 1 to 2650)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   6  (bases 1 to 2650)
  AUTHORS   Valdez,B.C., Perlaky,L., Saijo,Y., Henning,D., Zhu,C., Busch,R.K.,
            Zhang,W.W. and Busch,H.
  TITLE     A region of antisense RNA from human p120 cDNA with high homology
            to mouse p120 cDNA inhibits NIH 3T3 proliferation
  JOURNAL   Cancer Res 52 (20), 5681-5686 (1992)
   PUBMED   1394192
REFERENCE   7  (bases 1 to 2650)
  AUTHORS   Busch,H.
  TITLE     The final common pathway of cancer
  JOURNAL   Cancer Res 50 (16), 4830-4838 (1990)
   PUBMED   2199030
  REMARK    Review article
REFERENCE   8  (bases 1 to 2650)
  AUTHORS   Larson,R.G., Henning,D., Haidar,M.A., Jhiang,S., Lin,W.L.,
            Zhang,W.W. and Busch,H.
  TITLE     Genomic structure of the human proliferating cell nucleolar protein
            P120
  JOURNAL   Cancer Commun 2 (2), 63-71 (1990)
   PUBMED   2372471
REFERENCE   9  (bases 1 to 2650)
  AUTHORS   Fonagy,A., Henning,D., Jhiang,S., Haidar,M., Busch,R.K., Larson,R.,
            Valdez,B. and Busch,H.
  TITLE     Cloning of the cDNA and sequence of the human proliferating-cell
            nucleolar protein P120
  JOURNAL   Cancer Commun 1 (4), 243-251 (1989)
   PUBMED   2576976
REFERENCE   10 (bases 1 to 2650)
  AUTHORS   Freeman,J.W., Busch,R.K., Gyorkey,F., Gyorkey,P., Ross,B.E. and
            Busch,H.
  TITLE     Identification and characterization of a human
            proliferation-associated nucleolar antigen with a molecular weight
            of 120,000 expressed in early G1 phase
  JOURNAL   Cancer Res 48 (5), 1244-1251 (1988)
   PUBMED   3422591
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC128183.1 and CX868076.1.
            
            On Jun 2, 2019 this sequence version replaced NM_001258308.1.
            
            Transcript Variant: This variant (3) uses an alternate splice site
            in the coding region, but maintains the reading frame, compared to
            variant 1. The encoded isoform (2) is longer than isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC000656.2 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000322166.10/ ENSP00000313272.6
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2560              BC128183.1         17-2576
            2561-2650           CX868076.1         26-115              c
FEATURES             Location/Qualifiers
     source          1..2650
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12p13.31"
     gene            1..2650
                     /gene="NOP2"
                     /gene_synonym="NOL1; NOP120; NSUN1; p120"
                     /note="NOP2 nucleolar protein"
                     /db_xref="GeneID:4839"
                     /db_xref="HGNC:HGNC:7867"
                     /db_xref="MIM:164031"
     exon            1..85
                     /gene="NOP2"
                     /gene_synonym="NOL1; NOP120; NSUN1; p120"
                     /inference="alignment:Splign:2.1.0"
     exon            86..192
                     /gene="NOP2"
                     /gene_synonym="NOL1; NOP120; NSUN1; p120"
                     /inference="alignment:Splign:2.1.0"
     CDS             90..2528
                     /gene="NOP2"
                     /gene_synonym="NOL1; NOP120; NSUN1; p120"
                     /note="isoform 2 is encoded by transcript variant 3;
                     nucleolar protein 2 homolog; nucleolar protein 1, 120kDa;
                     NOP2 nucleolar protein homolog; probable 28S rRNA
                     (cytosine(4447)-C(5))-methyltransferase; NOL1/NOP2/Sun
                     domain family, member 1; putative ribosomal RNA
                     methyltransferase NOP2; proliferating-cell nucleolar
                     antigen p120; proliferation-associated nucleolar protein
                     p120"
                     /codon_start=1
                     /product="28S rRNA (cytosine(4447)-C(5))-methyltransferase
                     isoform 2"
                     /protein_id="NP_001245237.1"
                     /db_xref="CCDS:CCDS58203.1"
                     /db_xref="GeneID:4839"
                     /db_xref="HGNC:HGNC:7867"
                     /db_xref="MIM:164031"
                     /translation="MGRKLDPTKEKRGPGRKARKQKGAETELVRFLPAVSDENSKRLS
                     SRARKRAAKRRLGSVEAPKTNKSPEAKPLPGKLPKGISAGAVQTAGKKGPQSLFNAPR
                     GKKRPAPGSDEEEEEEDSEEDGMVNHGDLWGSEDDADTVDDYGADSNSEDEEEGEALL
                     PIERAARKQKAREAAAGIQWSEEETEDEEEEKEVTPESGPPKVEEADGGLQINVDEEP
                     FVLPPAGEMEQDAQAPDLQRVHKRIQDIVGILRDFGAQREEGRSRSEYLNRLKKDLAI
                     YYSYGDFLLGKLMDLFPLSELVEFLEANEVPRPVTLRTNTLKTRRRDLAQALINRGVN
                     LDPLGKWSKTGLVVYDSSVPIGATPEYLAGHYMLQGASSMLPVMALAPQEHERILDMC
                     CAPGGKTSYMAQLMKNTGVILANDANAERLKSVVGNLHRLGVTNTIISHYDGRQFPKV
                     VGGFDRVLLDAPCSGTGVISKDPAVKTNKDEKDILRCAHLQKELLLSAIDSVNATSKT
                     GGYLVYCTCSITVEENEWVVDYALKKRNVRLVPTGLDFGQEGFTRFRERRFHPSLRST
                     RRFYPHTHNMDGFFIAKFKKFSNSIPQSQTGNSETATPTNVDLPQVIPKSENSSQPAK
                     KAKGAAKTKQQLQKQQHPKKASFQKLNGISKGADSELSTVPSVTKTQASSSFQDSSQP
                     AGKAEGIREPKVTGKLKQRSPKLQSSKKVAFLRQNAPPKGTDTQTPAVLSPSKTQATL
                     KPKDHHQPLGRAKGVEKQQLPEQPFEKAAFQKQNDTPKGPQPPTVSPIRSSRPPPAKR
                     KKSQSRGNSQLLLS"
     exon            193..238
                     /gene="NOP2"
                     /gene_synonym="NOL1; NOP120; NSUN1; p120"
                     /inference="alignment:Splign:2.1.0"
     exon            239..327
                     /gene="NOP2"
                     /gene_synonym="NOL1; NOP120; NSUN1; p120"
                     /inference="alignment:Splign:2.1.0"
     exon            328..563
                     /gene="NOP2"
                     /gene_synonym="NOL1; NOP120; NSUN1; p120"
                     /inference="alignment:Splign:2.1.0"
     exon            564..619
                     /gene="NOP2"
                     /gene_synonym="NOL1; NOP120; NSUN1; p120"
                     /inference="alignment:Splign:2.1.0"
     exon            620..777
                     /gene="NOP2"
                     /gene_synonym="NOL1; NOP120; NSUN1; p120"
                     /inference="alignment:Splign:2.1.0"
     exon            778..977
                     /gene="NOP2"
                     /gene_synonym="NOL1; NOP120; NSUN1; p120"
                     /inference="alignment:Splign:2.1.0"
     exon            978..1067
                     /gene="NOP2"
                     /gene_synonym="NOL1; NOP120; NSUN1; p120"
                     /inference="alignment:Splign:2.1.0"
     exon            1068..1155
                     /gene="NOP2"
                     /gene_synonym="NOL1; NOP120; NSUN1; p120"
                     /inference="alignment:Splign:2.1.0"
     exon            1156..1296
                     /gene="NOP2"
                     /gene_synonym="NOL1; NOP120; NSUN1; p120"
                     /inference="alignment:Splign:2.1.0"
     exon            1297..1436
                     /gene="NOP2"
                     /gene_synonym="NOL1; NOP120; NSUN1; p120"
                     /inference="alignment:Splign:2.1.0"
     exon            1437..1526
                     /gene="NOP2"
                     /gene_synonym="NOL1; NOP120; NSUN1; p120"
                     /inference="alignment:Splign:2.1.0"
     exon            1527..1649
                     /gene="NOP2"
                     /gene_synonym="NOL1; NOP120; NSUN1; p120"
                     /inference="alignment:Splign:2.1.0"
     exon            1650..1878
                     /gene="NOP2"
                     /gene_synonym="NOL1; NOP120; NSUN1; p120"
                     /inference="alignment:Splign:2.1.0"
     exon            1879..2650
                     /gene="NOP2"
                     /gene_synonym="NOL1; NOP120; NSUN1; p120"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2629..2634
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NOP2"
                     /gene_synonym="NOL1; NOP120; NSUN1; p120"
                     /note="hexamer: AATACA"
     polyA_site      2650
                     /gene="NOP2"
                     /gene_synonym="NOL1; NOP120; NSUN1; p120"
                     /note="major polyA site"
ORIGIN      
        1 gtggcgagtg ccggccgaaa gctaggtccg gattgcacgt ggagggccgc ccgaagggca
       61 ctctcggaca ttaacccgca ttctgtacca tggggcgcaa gttggaccct acgaaggaga
      121 agcgggggcc aggccgaaag gcccggaagc agaagggtgc cgagacagaa ctcgtcagat
      181 tcttgcctgc agtaagtgac gaaaattcca agaggctgtc tagtcgtgct cgaaagaggg
      241 cagccaagag gagattgggc tctgttgaag cccctaagac aaataagtct cctgaggcca
      301 aaccattgcc tggaaagcta ccaaaaggga tctctgcagg agctgtccag acagctggta
      361 agaagggacc ccagtcccta tttaatgctc ctcgaggcaa gaagcgccca gcacctggca
      421 gtgatgagga agaggaggag gaagactctg aagaagatgg tatggtgaac cacggggacc
      481 tctggggctc cgaggacgat gctgatacgg tagatgacta tggagctgac tccaactctg
      541 aggatgagga ggaaggtgaa gcgttgctgc ccattgaaag agctgctcgg aagcagaagg
      601 cccgggaagc tgctgctggg atccagtgga gtgaagagga gaccgaggac gaggaggaag
      661 agaaagaagt gacccctgag tcaggccccc caaaggtgga agaggcagat gggggcctgc
      721 agatcaatgt ggatgaggaa ccatttgtgc tgccccctgc tggggagatg gagcaggatg
      781 cccaggctcc agacctgcaa cgagttcaca agcggatcca ggatattgtg ggaattctgc
      841 gtgattttgg ggctcagcgg gaggaagggc ggtctcgttc tgaatacctg aaccggctca
      901 agaaggatct ggccatttac tactcctatg gagacttcct gcttggcaag ctcatggacc
      961 tcttccctct gtctgagctg gtggagttct tagaagctaa tgaggtgcct cggcccgtca
     1021 ccctccggac caataccttg aaaacccgac gccgagacct tgcacaggct ctaatcaatc
     1081 gtggggttaa cctggatccc ctgggcaagt ggtcaaagac tggactagtg gtgtatgatt
     1141 cttctgtgcc cattggtgct acccccgagt acctggctgg gcactacatg ctgcagggag
     1201 cctccagcat gttgcccgtc atggccttgg caccccagga acatgagcgg atcctggaca
     1261 tgtgttgtgc ccctggagga aagaccagct acatggccca gctgatgaag aacacgggtg
     1321 tgatccttgc caatgacgcc aatgctgagc ggctcaagag tgttgtgggc aacttgcatc
     1381 ggctgggagt caccaacacc attatcagcc actatgatgg gcgccagttc cccaaggtgg
     1441 tggggggctt tgaccgagta ctgctggatg ctccctgcag tggcactggg gtcatctcca
     1501 aggatccagc cgtgaagact aacaaggatg agaaggacat cctgcgctgt gctcacctcc
     1561 agaaggagtt gctcctgagt gctattgact ctgtcaatgc gacctccaag acaggaggct
     1621 acctggttta ctgcacctgt tctatcacag tagaagagaa tgagtgggtg gtagactatg
     1681 ctctgaaaaa gaggaatgtg cgactggtgc ccacgggcct agactttggc caggaaggtt
     1741 ttacccgctt tcgagaaagg cgcttccacc ccagtctgcg ttctacccga cgcttctacc
     1801 ctcataccca caatatggat gggttcttca ttgccaagtt caagaaattt tccaattcta
     1861 tccctcagtc ccagacagga aattctgaaa cagccacacc tacaaatgta gacttgcctc
     1921 aggtcatccc caagtctgag aacagcagcc agccagccaa gaaagccaag ggggctgcaa
     1981 agacaaagca gcagctgcag aaacagcaac atcccaagaa ggcctccttc cagaagctga
     2041 atggcatctc caaaggggca gactcagaat tgtccactgt accttctgtc acaaagaccc
     2101 aagcttcctc cagcttccag gatagcagtc agccagctgg aaaagccgaa gggatcaggg
     2161 agccaaaggt gactgggaag ctaaagcaac gatcacctaa attacagtcc tccaagaaag
     2221 ttgctttcct caggcagaat gcccctccca agggcacaga cacacaaaca ccggctgtgt
     2281 tatccccatc caagactcag gccaccctga aacctaagga ccatcatcag ccccttggaa
     2341 gggccaaggg ggttgagaag cagcagttgc cagagcagcc ttttgagaaa gctgccttcc
     2401 agaaacagaa tgataccccc aaggggcctc agcctcccac tgtgtctccc atccgttcca
     2461 gccgcccccc accagcaaag aggaagaaat ctcagtccag gggcaacagc cagctgctgc
     2521 tatcttagat ggttgaaaac tagacgggtg gctcactgcc attgtcacca ggttggaact
     2581 cttgcctctg tgaggatgcc ttctctactg tgcataccca tgaaatttaa tacacatttt
     2641 aaaacctctg
//
