LOCUS       NM_024654               6627 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens nucleolar protein 9 (NOL9), mRNA.
ACCESSION   NM_024654
VERSION     NM_024654.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6627)
  AUTHORS   Gordon,J., Pillon,M.C. and Stanley,R.E.
  TITLE     Nol9 Is a Spatial Regulator for the Human ITS2 Pre-rRNA
            Endonuclease-Kinase Complex
  JOURNAL   J Mol Biol 431 (19), 3771-3786 (2019)
   PUBMED   31288032
  REMARK    GeneRIF: a Nol9-encoded nucleolar localization sequence that is
            responsible for nucleolar transport of the assembled Las1L-Nol9
            complex, is reported.
REFERENCE   2  (bases 1 to 6627)
  AUTHORS   Yadav,L., Tamene,F., Goos,H., van Drogen,A., Katainen,R.,
            Aebersold,R., Gstaiger,M. and Varjosalo,M.
  TITLE     Systematic Analysis of Human Protein Phosphatase Interactions and
            Dynamics
  JOURNAL   Cell Syst 4 (4), 430-444 (2017)
   PUBMED   28330616
REFERENCE   3  (bases 1 to 6627)
  AUTHORS   Bielczyk-Maczynska,E., Lam Hung,L., Ferreira,L., Fleischmann,T.,
            Weis,F., Fernandez-Pevida,A., Harvey,S.A., Wali,N., Warren,A.J.,
            Barroso,I., Stemple,D.L. and Cvejic,A.
  TITLE     The Ribosome Biogenesis Protein Nol9 Is Essential for Definitive
            Hematopoiesis and Pancreas Morphogenesis in Zebrafish
  JOURNAL   PLoS Genet 11 (12), e1005677 (2015)
   PUBMED   26624285
  REMARK    GeneRIF: we present the nol9sa1022/sa1022 mutant, a novel zebrafish
            ribosomopathy model, which recapitulates key human disease
            characteristics.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6627)
  AUTHORS   Heindl,K. and Martinez,J.
  TITLE     Nol9 is a novel polynucleotide 5'-kinase involved in ribosomal RNA
            processing
  JOURNAL   EMBO J 29 (24), 4161-4171 (2010)
   PUBMED   21063389
  REMARK    GeneRIF: The polynucleotide kinase activity of Nol9 is required for
            efficient generation of the 5.8S and 28S ribosomal RNAs from the
            32S precursor.
REFERENCE   5  (bases 1 to 6627)
  AUTHORS   Ghosh,D., Lippert,D., Krokhin,O., Cortens,J.P. and Wilkins,J.A.
  TITLE     Defining the membrane proteome of NK cells
  JOURNAL   J Mass Spectrom 45 (1), 1-25 (2010)
   PUBMED   19946888
REFERENCE   6  (bases 1 to 6627)
  AUTHORS   Scherl,A., Coute,Y., Deon,C., Calle,A., Kindbeiter,K.,
            Sanchez,J.C., Greco,A., Hochstrasser,D. and Diaz,J.J.
  TITLE     Functional proteomic analysis of human nucleolus
  JOURNAL   Mol Biol Cell 13 (11), 4100-4109 (2002)
   PUBMED   12429849
REFERENCE   7  (bases 1 to 6627)
  AUTHORS   Wistow,G., Bernstein,S.L., Wyatt,M.K., Fariss,R.N., Behal,A.,
            Touchman,J.W., Bouffard,G., Smith,D. and Peterson,K.
  TITLE     Expressed sequence tag analysis of human RPE/choroid for the
            NEIBank Project: over 6000 non-redundant transcripts, novel genes
            and splice variants
  JOURNAL   Mol Vis 8, 205-220 (2002)
   PUBMED   12107410
  REMARK    Publication Status: Online-Only
REFERENCE   8  (bases 1 to 6627)
  AUTHORS   Andersen,J.S., Lyon,C.E., Fox,A.H., Leung,A.K., Lam,Y.W., Steen,H.,
            Mann,M. and Lamond,A.I.
  TITLE     Directed proteomic analysis of the human nucleolus
  JOURNAL   Curr Biol 12 (1), 1-11 (2002)
   PUBMED   11790298
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AL591866.13.
            
            On Nov 23, 2018 this sequence version replaced NM_024654.4.
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK091284.1, SRR1660803.101026.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000377705.6/ ENSP00000366934.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-407               AL591866.13        51297-51703         c
            408-627             AL591866.13        47586-47805         c
            628-755             AL591866.13        46761-46888         c
            756-891             AL591866.13        42235-42370         c
            892-988             AL591866.13        42016-42112         c
            989-1086            AL591866.13        39020-39117         c
            1087-1248           AL591866.13        30470-30631         c
            1249-1546           AL591866.13        29653-29950         c
            1547-1658           AL591866.13        29158-29269         c
            1659-1836           AL591866.13        26184-26361         c
            1837-1970           AL591866.13        23886-24019         c
            1971-6627           AL591866.13        18537-23193         c
FEATURES             Location/Qualifiers
     source          1..6627
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1p36.31"
     gene            1..6627
                     /gene="NOL9"
                     /gene_synonym="Grc3; NET6"
                     /note="nucleolar protein 9"
                     /db_xref="GeneID:79707"
                     /db_xref="HGNC:HGNC:26265"
                     /db_xref="MIM:620304"
     exon            1..407
                     /gene="NOL9"
                     /gene_synonym="Grc3; NET6"
                     /inference="alignment:Splign:2.1.0"
     CDS             12..2120
                     /gene="NOL9"
                     /gene_synonym="Grc3; NET6"
                     /EC_number="2.7.1.78"
                     /note="polynucleotide 5'-kinase"
                     /codon_start=1
                     /product="polynucleotide 5'-hydroxyl-kinase NOL9"
                     /protein_id="NP_078930.4"
                     /db_xref="CCDS:CCDS80.1"
                     /db_xref="GeneID:79707"
                     /db_xref="HGNC:HGNC:26265"
                     /db_xref="MIM:620304"
                     /translation="MADSGLLLKRGSCRSTWLRVRKARPQLILSRRPRRRLGSLRWCG
                     RRRLRWRLLQAQASGVDWREGARQVSRAAAARRPNTATPSPIPSPTPASEPESEPELE
                     SASSCHRPLLIPPVRPVGPGRALLLLPVEQGFTFSGICRVTCLYGQVQVFGFTISQGQ
                     PAQDIFSVYTHSCLSIHALHYSQPEKSKKELKREARNLLKSHLNLDDRRWSMQNFSPQ
                     CSIVLLEHLKTATVNFITSYPGSSYIFVQESPTPQIKPEYLALRSVGIRREKKRKGLQ
                     LTESTLSALEELVNVSCEEVDGCPVILVCGSQDVGKSTFNRYLINHLLNSLPCVDYLE
                     CDLGQTEFTPPGCISLLNITEPVLGPPFTHLRTPQKMVYYGKPSCKNNYENYIDIVKY
                     VFSAYKRESPLIVNTMGWVSDQGLLLLIDLIRLLSPSHVVQFRSDHSKYMPDLTPQYV
                     DDMDGLYTKSKTKMRNRRFRLAAFADALEFADEEKESPVEFTGHKLIGVYTDFAFRIT
                     PRNRESHNKILRDLSILSYLSQLQPPMPKPLSPLHSLTPYQVPFNAVALRITHSDVAP
                     THILYAVNASWVGLCKIQDDVRGYTNGPILLAQTPICDCLGFGICRGIDMEKRLYHIL
                     TPVPPEELRTVNCLLVGAIAIPHCVLKCQRGIEGTVPYVTTDYNFKLPGASEKIGARE
                     PEEAHKEKPYRRPKFCRKMK"
     exon            408..627
                     /gene="NOL9"
                     /gene_synonym="Grc3; NET6"
                     /inference="alignment:Splign:2.1.0"
     exon            628..755
                     /gene="NOL9"
                     /gene_synonym="Grc3; NET6"
                     /inference="alignment:Splign:2.1.0"
     exon            756..891
                     /gene="NOL9"
                     /gene_synonym="Grc3; NET6"
                     /inference="alignment:Splign:2.1.0"
     exon            892..988
                     /gene="NOL9"
                     /gene_synonym="Grc3; NET6"
                     /inference="alignment:Splign:2.1.0"
     exon            989..1086
                     /gene="NOL9"
                     /gene_synonym="Grc3; NET6"
                     /inference="alignment:Splign:2.1.0"
     exon            1087..1248
                     /gene="NOL9"
                     /gene_synonym="Grc3; NET6"
                     /inference="alignment:Splign:2.1.0"
     exon            1249..1546
                     /gene="NOL9"
                     /gene_synonym="Grc3; NET6"
                     /inference="alignment:Splign:2.1.0"
     exon            1547..1658
                     /gene="NOL9"
                     /gene_synonym="Grc3; NET6"
                     /inference="alignment:Splign:2.1.0"
     exon            1659..1836
                     /gene="NOL9"
                     /gene_synonym="Grc3; NET6"
                     /inference="alignment:Splign:2.1.0"
     exon            1837..1970
                     /gene="NOL9"
                     /gene_synonym="Grc3; NET6"
                     /inference="alignment:Splign:2.1.0"
     exon            1971..6627
                     /gene="NOL9"
                     /gene_synonym="Grc3; NET6"
                     /inference="alignment:Splign:2.1.0"
     polyA_site      2307
                     /gene="NOL9"
                     /gene_synonym="Grc3; NET6"
     regulatory      2384..2389
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NOL9"
                     /gene_synonym="Grc3; NET6"
                     /note="hexamer: ATTAAA"
     polyA_site      2407
                     /gene="NOL9"
                     /gene_synonym="Grc3; NET6"
     regulatory      6604..6609
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NOL9"
                     /gene_synonym="Grc3; NET6"
                     /note="hexamer: ATTAAA"
     polyA_site      6627
                     /gene="NOL9"
                     /gene_synonym="Grc3; NET6"
                     /note="major polyA site"
ORIGIN      
        1 gaggacccag catggcggac tcgggactgc tgctaaagcg gggttcctgc cgttccactt
       61 ggctgcgggt ccgcaaggcc cggccccagc tcatcctcag ccgccggccc cgccgccggc
      121 tcgggagcct gcgctggtgc ggtcggcggc gcctacggtg gcggttactg caagcccagg
      181 cgtccggcgt ggactggagg gagggagccc gccaggtgtc gcgcgcggcg gcggcccgga
      241 gacccaacac cgcgaccccc agcccgatcc ctagcccgac cccggcctcc gaacccgagt
      301 ccgaacccga actcgaatcc gcctcgagtt gccaccggcc tctcctcatc ccaccggtgc
      361 ggcccgtggg ccccggccgc gcgttgctgc tgctgccggt cgagcagggt tttactttta
      421 gtgggatctg tcgtgtgact tgcctctatg gccaggtgca ggtatttggt tttaccatca
      481 gccaaggcca gcctgcccaa gacatcttct ctgtgtatac ccactcttgc ttgagtatcc
      541 atgcacttca ctactcacag cctgagaaaa gcaagaagga actgaaaagg gaagcccgga
      601 atttgctcaa atctcatctt aaccttgatg acaggcgttg gtcgatgcag aatttttctc
      661 ctcagtgttc cattgtgttg ctagaacatc tgaaaactgc cactgtaaac ttcataacca
      721 gctatccggg ttcatcctac atttttgtgc aagagagtcc aactccccag attaaacctg
      781 aatatttagc cttgaggtct gttggcatca gaagagagaa aaaaaggaaa ggccttcagt
      841 taactgagag taccctttca gccctggaag agttagtcaa tgtttcctgt gaagaagtag
      901 atggctgccc tgtcattcta gtttgtggat cccaggatgt tggaaagtca acatttaata
      961 gatacctgat taaccatttg ttaaatagtc ttccctgcgt tgactatttg gaatgtgatc
     1021 tgggacagac agaatttacc cctcctggtt gcatttcttt gcttaatatt acagaaccag
     1081 ttctgggacc acctttcact cacctgagga ctccacagaa gatggtatat tatgggaaac
     1141 cttcttgtaa aaacaactat gagaattata ttgacatagt gaaatatgtg ttcagcgctt
     1201 acaagagaga gtcccctctc atcgtcaaca ctatgggatg ggtttcagac caggggctcc
     1261 tgcttctcat tgatctgatc cgattgctgt ctcccagcca cgtggttcag ttccgctctg
     1321 accacagtaa atatatgcca gaccttaccc cgcagtatgt agatgacatg gatggcttgt
     1381 acacaaaaag caagaccaag atgagaaatc gacgtttcag actcgcagca tttgcagatg
     1441 ctttggaatt tgctgatgaa gaaaaagaga gtccagttga gttcactgga cataaactga
     1501 taggtgttta tacagacttt gcattcagaa taactccaag aaatagagag tcacataaca
     1561 aaattcttcg agatctgtcc atcttgagtt accttagcca gctgcagccc ccgatgccca
     1621 aaccactttc tcctttacat agcctgacac cctatcaggt ccctttcaat gcagtcgcac
     1681 tccggattac ccactctgat gtcgccccta cccatatact atatgctgta aacgccagct
     1741 gggttggtct ttgcaagatc caggatgacg tcagaggata cacgaatggg cccatcctgc
     1801 ttgcccagac tccaatctgt gactgcttgg gctttggcat ctgtagaggc attgacatgg
     1861 agaagcggct gtaccacatc ctcacccctg tgcccccgga agagctaagg accgtgaatt
     1921 gtctgctcgt tggagctatt gccattccac attgtgtcct taagtgccag cgtgggatcg
     1981 aagggacagt accttatgtc acaacggatt acaattttaa acttcctgga gcatcagaga
     2041 aaattggagc aagagaacct gaggaggcac ataaagagaa accataccga agacctaagt
     2101 tctgtcgaaa aatgaagtga tgctcgcgtt tttaataagg gaagaaactt tcctaccaga
     2161 aagctttgtc tcaagcctga ccacaagaga catgatggag tttcatggcc atgaatggtg
     2221 cccttattag caacagtgtt ttttgtgtaa ttcgtgaatg tcatatagta gtcttaagtt
     2281 tctcttttaa agctgcatga gctagaattt gttctagtct tctgtgtgat actcatactt
     2341 gctgtccaga gggagtaaaa cagcagagaa cagtttgtgt gccattaaaa cagagattcc
     2401 tcagaaagtt cttcagggac ctggttatta gaatcaggat aaaaataagg aaactggggc
     2461 cattacaatg taccgagaag gaaggaagaa ggccgacctg caggatctca agcataatgg
     2521 atacaagggc tcgccacggg ccttgcctga gccatgtgca gagctggggt ccagcggtct
     2581 gcttccttgg agccagtgtg gatggttaca gcccatccat ttggtgcagt tgtacttggt
     2641 ctgttatatt caatcaaggt ttaatagata gggctgggca tggtggctca tgcacacctg
     2701 taatcctagc actttgggag gctgaggcgg gaggattgct tgagcctcgg agttccaggc
     2761 tggagtacac caactactcc agcctgagcg acagagtaag accttatctt ttaaaaaaga
     2821 aaagaaaaat ttaaacgggt ttgatagata taaatttgga cccaagagcc cttccttttt
     2881 tttaatcctg aggttttatg ctgaggaaat ttctttcaaa tctggaaaac ctcggggcca
     2941 ggcgcagtgg ctcacgcctg taattccagc actttgggag gccaaggcag gcggttcacg
     3001 aggtcaggag attgagacca tcctggctaa cacagtgaaa ctccgtctct actaaaaata
     3061 caaaaaacta gctgggcgtg gtggtgggcg cctatagtcc cagctactca ggaggctgag
     3121 gcaggagaat ggcatgaacc caggaggcgg agcttgcagt gagccgaggt cacgccactg
     3181 cactccagcc tgggcaacaa gagcaagact ccatctcaaa aaaaaaaaat aataatctgg
     3241 aaaacctctt ctgggggctc ttataaaagt agatttgtcc aaattgactt ctgatctgag
     3301 gccagagaca accacagggc agagctggag actgggctgt tttgaaggaa gaaggactgg
     3361 cagaccagct cttgggaaag ctccattatt gcctgtggga ggctggacct gaacttgcac
     3421 ctatgaacaa ccctgccatt tagagctttt tcctgggggt gccggcggct ggtgaccaga
     3481 ctgccattct ccacgttctc cctggtgctt ccgactctgt cctctaggtt ggaagtggtt
     3541 tgtgtaaacg ggtgatggga ccaggtcaca tcatttctgc atttaagaca gcaggatact
     3601 tcctggggct gaggttcaaa ctggtattgt gaccaccgct ttgacagaat tccatcttcc
     3661 cagcatgatc acattgtaag actattagga tgtgtgggtt ttaacactta tatacactta
     3721 tacaactgtt ggttatcatt gtctttagta ttttacagtg gcccctcagc acctcttaga
     3781 cccaacagca aaggacttaa aataagcaca ggaaaccttg ctatggtgat tagttttgat
     3841 acctttgaga caattgagtc tagcttctta gttgcccctt atttatgttg aaatctggct
     3901 cattcatatg atgttttact gtgctggcct tgtaaagaat tttaagacaa tccatcacca
     3961 tcaatgtttt gcctttctcc actttcgtgt atcttacagt tatactcact ggaggtcatt
     4021 tgcagcaagg ctctggaaga atgaaactgg tgccctgtaa ttgcatttgc attcaatcta
     4081 ggtttgcctt caggctttaa atgcggcctg tatgaaggtc aagtcttttt cttcagtccc
     4141 tatcagtaaa gaaaaatcgt tgcctctaga gggagctatc agctgtgggt caggtggccg
     4201 tactggggct agagttggcc caggaagctg tacagggttt agggaaggag gttcggagac
     4261 agggagccaa ggcctctgtc aggtctagct tgtgccggtc agacctgcag cagcaccatt
     4321 ctccaggatg acaattcatc ccattattga tggcagtggc ctttttatcc tttttttttt
     4381 tttttttttt ttttgagatg gagtttcgct cttgttgccc aggctggagt gcagtggcgc
     4441 aatctgggct cactgccacc tccgcctccc gggttcaagt gattctcttg cctcaacctc
     4501 ccgagtagct gggattacag gcatgcgcca ccatgcctgg ctgattttct atttttagta
     4561 gagaaggggt ttcaccatgt tggccaggct ggttttgaac tcctgtcctc aggtgatcca
     4621 cctgccttgg cctcccaaag tgttgggatt acaggcatga gccaccgcac ccggctggtg
     4681 gcagtggctt taagggcatt tagaagaagg acaagattgg actttttatc atttccacag
     4741 tctgttatgg gtcagattag gtagtaagga gttctccatt ggagctattc tttttttttt
     4801 ttcttttttt ttgagacaga gtctcgctct gtcacccagg ctggggtgca gtggcacgac
     4861 cttggctcac tacagcctct cccctcccgg gttcaagtga ttcttctgcc ccagcctccc
     4921 aagtagctgg gattacaggt gagcgccacc atgcctggct aatttttata tttttagtag
     4981 agatagggtt tcaccgtatt ggctaggctg gtctcgatct cctgaccttg tgatccgctt
     5041 gcctcggcct cccaaagtgc tgaagcaatt tctttttttt tttttttttt gagacagagt
     5101 ctagctctgt cgcccaggct ggggtgcagt ggagtgatct cggctcactg caacttccgc
     5161 ctcccaggtt taagcgattc tcctgcctca gcctcctgag tagctgggat tacaggcgta
     5221 tgccaccacg cccagctaat ttttgtattt ttagtagaga cagggtttca ccatgttggt
     5281 caggctggtc tcgaactcct gacctcgtga tccgcccgcc ttggcctccg aaagtgctgg
     5341 gattacaggc gtgagccact gcgcctggcc tgaagctatt ctttacaaaa gctcttagtt
     5401 tttgtttgtt tgtttgtttt tttcttttgc gacggactct ctgtcgccaa ggctggagtg
     5461 cagtggcctg atctcaggtc actgcaacct ctgcctcctg ggttcaagcg attctcctgc
     5521 ttcagcctcc caagtagctg ggattacagg cgcctgccac cacacccagc taatttttgt
     5581 atttttagta gagatggcgt ttcactttat tggtcaggct ggtctcgaac ttccaacctc
     5641 aggggatccg cctgccttgg cctcccaagg tgttgggatt acaggcataa gacactgtgc
     5701 ctgggcctct tagtaatttt tgtatgtaat tttcaaagta tccgggatta caggcgcccg
     5761 ccactacact agctaatttt ttttgtattt ttagtagaga tggggtttta ctatgttggc
     5821 taggctggtc tcgagctcct gacctcgtga tccgtccacc ttggcctccc aaagtgctgg
     5881 gattacaggc gtgagccacc gcgcccggcc taggtcattt gtttttgggt ttggatggca
     5941 agatcatgga taagacagca ggctttgatt atagagctct gagttcaaat cctggctcta
     6001 ccacttgtca gtggtgtaac ttggaggaag atgcttagcc tgagcctgtt ttatgttttg
     6061 aagattgggt aattgtcctt ccttcagtgt tcctgggtga ggagtaagtg aagtcatgtc
     6121 tataagagat gtagcacagt ctccggcatg tagaaacggt cagtaactcc tagtgtgtag
     6181 ctagcctctg atgagatggt aaactcagga ggctggcacc acggctgtgc tactcacggt
     6241 catgctggag ggatgcagaa actaaatgaa tccacagcta cttactcatc ttgcctctgg
     6301 caggttcacc ttaaaattgt tgctaccaag cttcctgtgg tcaaagagcc cttgaggaag
     6361 caagctccac ggccaccata gataacccat tcctgaaagt ttttcataaa cccccacttg
     6421 ttgcttttaa aattttataa gcagcttaca catgtagaaa atgatcctcc agcccaccac
     6481 cacatagtaa tcactgacac tgtgaggtgt ctcagctggg gttctgctgt gtgtgtgtgt
     6541 attttacaaa atatgtatgg tttctgttca gctcttttca gttaacagat gaacttttgt
     6601 atcattaaat atttttgaaa acatgaa
//
