LOCUS       NM_001331026            6191 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens nicotinamide nucleotide transhydrogenase (NNT),
            transcript variant 3, mRNA; nuclear gene for mitochondrial product.
ACCESSION   NM_001331026 XM_011514002
VERSION     NM_001331026.2
KEYWORDS    RefSeq.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6191)
  AUTHORS   Francisco,A., Goler,A.M.Y., Navarro,C.D.C., Onder,A., Yildiz,M.,
            Kendir Demirkol,Y., Karademir Yilmaz,B., Seven Menevse,T., Guran,T.
            and Castilho,R.F.
  TITLE     Lack of NAD(P)+ transhydrogenase activity in patients with primary
            adrenal insufficiency due to NNT variants
  JOURNAL   Eur J Endocrinol 190 (2), 130-138 (2024)
   PUBMED   38261461
  REMARK    GeneRIF: Lack of NAD(P)+ transhydrogenase activity in patients with
            primary adrenal insufficiency due to NNT variants.
            Erratum:[Eur J Endocrinol. 2024 Sep 30;191(4):X3. doi:
            10.1093/ejendo/lvae118.. PMID: 39361449]
REFERENCE   2  (bases 1 to 6191)
  AUTHORS   Grayson,C. and Mailloux,R.J.
  TITLE     Coenzyme Q10 and nicotinamide nucleotide transhydrogenase:
            Sentinels for mitochondrial hydrogen peroxide signaling
  JOURNAL   Free Radic Biol Med 208, 260-271 (2023)
   PUBMED   37573896
  REMARK    GeneRIF: Coenzyme Q10 and nicotinamide nucleotide transhydrogenase:
            Sentinels for mitochondrial hydrogen peroxide signaling.
            Review article
REFERENCE   3  (bases 1 to 6191)
  AUTHORS   Han,Y., Zhang,Y.Y., Pan,Y.Q., Zheng,X.J., Liao,K., Mo,H.Y.,
            Sheng,H., Wu,Q.N., Liu,Z.X., Zeng,Z.L., Yang,W., Yuan,S.Q.,
            Huang,P., Ju,H.Q. and Xu,R.H.
  TITLE     IL-1beta-associated NNT acetylation orchestrates iron-sulfur
            cluster maintenance and cancer immunotherapy resistance
  JOURNAL   Mol Cell 83 (11), 1887-1902 (2023)
   PUBMED   37244254
  REMARK    GeneRIF: IL-1beta-associated NNT acetylation orchestrates
            iron-sulfur cluster maintenance and cancer immunotherapy
            resistance.
REFERENCE   4  (bases 1 to 6191)
  AUTHORS   Bodoni,A.F., Coeli-Lacchini,F.B., Gebenlian,J.L., Sobral,L.M.,
            Garcia,C.B., Silva,W.A., Peronni,K.C., Ramalho,L.N.Z.,
            Ramalho,F.S., Moreira,A.C., de Castro,M., Leopoldino,A.M. and
            Antonini,S.R.R.
  TITLE     Nicotinamide Nucleotide Transhydrogenase Is Essential for Adrenal
            Steroidogenesis: Clinical and In Vitro Lessons
  JOURNAL   J Clin Endocrinol Metab 108 (6), 1464-1474 (2023)
   PUBMED   36478070
  REMARK    GeneRIF: Nicotinamide Nucleotide Transhydrogenase Is Essential for
            Adrenal Steroidogenesis: Clinical and In Vitro Lessons.
REFERENCE   5  (bases 1 to 6191)
  AUTHORS   Krasovec,T., Sikonja,J., Zerjav Tansek,M., Debeljak,M., Ilovar,S.,
            Trebusak Podkrajsek,K., Bertok,S., Tesovnik,T., Kovac,J., Suput
            Omladic,J., Hartmann,M.F., Wudy,S.A., Avbelj Stefanija,M.,
            Battelino,T., Kotnik,P. and Groselj,U.
  TITLE     Long-Term Follow-Up of Three Family Members with a Novel NNT
            Pathogenic Variant Causing Primary Adrenal Insufficiency
  JOURNAL   Genes (Basel) 13 (5), 717 (2022)
   PUBMED   35627102
  REMARK    GeneRIF: Long-Term Follow-Up of Three Family Members with a Novel
            NNT Pathogenic Variant Causing Primary Adrenal Insufficiency.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 6191)
  AUTHORS   White,S.A., Peake,S.J., McSweeney,S., Leonard,G., Cotton,N.P. and
            Jackson,J.B.
  TITLE     The high-resolution structure of the NADP(H)-binding component
            (dIII) of proton-translocating transhydrogenase from human heart
            mitochondria
  JOURNAL   Structure 8 (1), 1-12 (2000)
   PUBMED   10673423
REFERENCE   7  (bases 1 to 6191)
  AUTHORS   Arkblad,E.L., Helou,K., Levan,G. and Rydstrom,J.
  TITLE     Mapping of the rat and mouse nicotinamide nucleotide
            transhydrogenase gene
  JOURNAL   Mamm Genome 8 (9), 703 (1997)
   PUBMED   9271681
REFERENCE   8  (bases 1 to 6191)
  AUTHORS   Zieger,B. and Ware,J.
  TITLE     Cloning and deduced amino acid sequence of human nicotinamide
            nucleotide transhydrogenase
  JOURNAL   DNA Seq 7 (6), 369-373 (1997)
   PUBMED   9524818
REFERENCE   9  (bases 1 to 6191)
  AUTHORS   Forsmark-Andree,P., Persson,B., Radi,R., Dallner,G. and Ernster,L.
  TITLE     Oxidative modification of nicotinamide nucleotide transhydrogenase
            in submitochondrial particles: effect of endogenous ubiquinol
  JOURNAL   Arch Biochem Biophys 336 (1), 113-120 (1996)
   PUBMED   8951041
REFERENCE   10 (bases 1 to 6191)
  AUTHORS   Arkblad,E.L., Betsholtz,C. and Rydstrom,J.
  TITLE     The cDNA sequence of proton-pumping nicotinamide nucleotide
            transhydrogenase from man and mouse
  JOURNAL   Biochim Biophys Acta 1273 (3), 203-205 (1996)
   PUBMED   8616157
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC010435.6.
            
            On May 31, 2019 this sequence version replaced NM_001331026.1.
            
            Summary: This gene encodes an integral protein of the inner
            mitochondrial membrane. The enzyme couples hydride transfer between
            NAD(H) and NADP(+) to proton translocation across the inner
            mitochondrial membrane. Under most physiological conditions, the
            enzyme uses energy from the mitochondrial proton gradient to
            produce high concentrations of NADPH. The resulting NADPH is used
            for biosynthesis and in free radical detoxification. [provided by
            RefSeq, Sep 2016].
            
            Transcript Variant: This variant (3) differs in the 5' UTR and uses
            a downstream in-frame start codon, compared to variant 1. The
            encoded isoform (2) has a shorter N-terminus, compared to isoform
            1.
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803615.264744.1,
                                           SRR1803613.135399.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1968968
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: PMID: 12592411; reported by
                                                  MitoCarta
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-115               AC010435.6         32667-32781
            116-319             AC010435.6         38630-38833
            320-537             AC010435.6         45335-45552
            538-625             AC010435.6         48519-48606
            626-714             AC010435.6         53519-53607
            715-902             AC010435.6         57687-57874
            903-1036            AC010435.6         73679-73812
            1037-1228           AC010435.6         74098-74289
            1229-1382           AC010435.6         74844-74997
            1383-1544           AC010435.6         78634-78795
            1545-1655           AC010435.6         79964-80074
            1656-1801           AC010435.6         81226-81371
            1802-1997           AC010435.6         82505-82700
            1998-2231           AC010435.6         85327-85560
            2232-2392           AC010435.6         86140-86300
            2393-2572           AC010435.6         88658-88837
            2573-2732           AC010435.6         104998-105157
            2733-2814           AC010435.6         107212-107293
            2815-2933           AC010435.6         129606-129724
            2934-3049           AC010435.6         132108-132223
            3050-6191           AC010435.6         133742-136883
FEATURES             Location/Qualifiers
     source          1..6191
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5p12"
     gene            1..6191
                     /gene="NNT"
                     /gene_synonym="GCCD4"
                     /note="nicotinamide nucleotide transhydrogenase"
                     /db_xref="GeneID:23530"
                     /db_xref="HGNC:HGNC:7863"
                     /db_xref="MIM:607878"
     exon            1..115
                     /gene="NNT"
                     /gene_synonym="GCCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            116..319
                     /gene="NNT"
                     /gene_synonym="GCCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            320..537
                     /gene="NNT"
                     /gene_synonym="GCCD4"
                     /inference="alignment:Splign:2.1.0"
     CDS             332..3199
                     /gene="NNT"
                     /gene_synonym="GCCD4"
                     /EC_number="7.1.1.1"
                     /note="isoform 2 is encoded by transcript variant 3;
                     pyridine nucleotide transhydrogenase; NAD(P)
                     transhydrogenase, mitochondrial"
                     /codon_start=1
                     /product="NAD(P) transhydrogenase, mitochondrial isoform
                     2"
                     /protein_id="NP_001317955.1"
                     /db_xref="CCDS:CCDS82994.1"
                     /db_xref="GeneID:23530"
                     /db_xref="HGNC:HGNC:7863"
                     /db_xref="MIM:607878"
                     /translation="MVNPTLGVHEADLLKTSGTLISFIYPAQNPELLNKLSQRKTTVL
                     AMDQVPRVTIAQGYDALSSMANIAGYKAVVLAANHFGRFFTGQITAAGKVPPAKILIV
                     GGGVAGLASAGAAKSMGAIVRGFDTRAAALEQFKSLGAEPLEVDLKESGEGQGGYAKE
                     MSKEFIEAEMKLFAQQCKEVDILISTALIPGKKAPVLFNKEMIESMKEGSVVVDLAAE
                     AGGNFETTKPGELYIHKGITHIGYTDLPSRMATQASTLYSNNITKLLKAISPDKDNFY
                     FDVKDDFDFGTMGHVIRGTVVMKDGKVIFPAPTPKNIPQGAPVKQKTVAELEAEKAAT
                     ITPFRKTMSTASAYTAGLTGILGLGIAAPNLAFSQMVTTFGLAGIVGYHTVWGVTPAL
                     HSPLMSVTNAISGLTAVGGLALMGGHLYPSTTSQGLAALAAFISSVNIAGGFLVTQRM
                     LDMFKRPTDPPEYNYLYLLPAGTFVGGYLAALYSGYNIEQIMYLGSGLCCVGALAGLS
                     TQGTARLGNALGMIGVAGGLAATLGVLKPGPELLAQMSGAMALGGTIGLTIAKRIQIS
                     DLPQLVAAFHSLVGLAAVLTCIAEYIIEYPHFATDAAANLTKIVAYLGTYIGGVTFSG
                     SLIAYGKLQGLLKSAPLLLPGRHLLNAGLLAASVGGIIPFMVDPSFTTGITCLGSVSA
                     LSAVMGVTLTAAIGGADMPVVITVLNSYSGWALCAEGFLLNNNLLTIVGALIGSSGAI
                     LSYIMCVAMNRSLANVILGGYGTTSTAGGKPMEISGTHTEINLDNAIDMIREANSIII
                     TPGYGLCAAKAQYPIADLVKMLTEQGKKVRFGIHPVAGRMPGQLNVLLAEAGVPYDIV
                     LEMDEINHDFPDTDLVLVIGANDTVNSAAQEDPNSIIAGMPVLEVWKSKQVIVMKRSL
                     GVGYAAVDNPIFYKPNTAMLLGDAKKTCDALQAKVRESYQK"
     exon            538..625
                     /gene="NNT"
                     /gene_synonym="GCCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            626..714
                     /gene="NNT"
                     /gene_synonym="GCCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            715..902
                     /gene="NNT"
                     /gene_synonym="GCCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            903..1036
                     /gene="NNT"
                     /gene_synonym="GCCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            1037..1228
                     /gene="NNT"
                     /gene_synonym="GCCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            1229..1382
                     /gene="NNT"
                     /gene_synonym="GCCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            1383..1544
                     /gene="NNT"
                     /gene_synonym="GCCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            1545..1655
                     /gene="NNT"
                     /gene_synonym="GCCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            1656..1801
                     /gene="NNT"
                     /gene_synonym="GCCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            1802..1997
                     /gene="NNT"
                     /gene_synonym="GCCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            1998..2231
                     /gene="NNT"
                     /gene_synonym="GCCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            2232..2392
                     /gene="NNT"
                     /gene_synonym="GCCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            2393..2572
                     /gene="NNT"
                     /gene_synonym="GCCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            2573..2732
                     /gene="NNT"
                     /gene_synonym="GCCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            2733..2814
                     /gene="NNT"
                     /gene_synonym="GCCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            2815..2933
                     /gene="NNT"
                     /gene_synonym="GCCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            2934..3049
                     /gene="NNT"
                     /gene_synonym="GCCD4"
                     /inference="alignment:Splign:2.1.0"
     exon            3050..6191
                     /gene="NNT"
                     /gene_synonym="GCCD4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4003..4008
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NNT"
                     /gene_synonym="GCCD4"
                     /note="hexamer: AATAAA"
     polyA_site      4029
                     /gene="NNT"
                     /gene_synonym="GCCD4"
                     /note="major polyA site"
     regulatory      4263..4268
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NNT"
                     /gene_synonym="GCCD4"
                     /note="hexamer: AGTAAA"
     polyA_site      4288
                     /gene="NNT"
                     /gene_synonym="GCCD4"
     regulatory      4333..4338
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NNT"
                     /gene_synonym="GCCD4"
                     /note="hexamer: AATATA"
     polyA_site      4355
                     /gene="NNT"
                     /gene_synonym="GCCD4"
     regulatory      5995..6000
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NNT"
                     /gene_synonym="GCCD4"
                     /note="hexamer: AAGAAA"
     polyA_site      6016
                     /gene="NNT"
                     /gene_synonym="GCCD4"
     regulatory      6171..6176
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NNT"
                     /gene_synonym="GCCD4"
                     /note="hexamer: AATACA"
     polyA_site      6191
                     /gene="NNT"
                     /gene_synonym="GCCD4"
ORIGIN      
        1 gctgttcttc cgggttggag gcgcagcgcc gcggggccca agcccgggtc tgccagcgcg
       61 acgtcctctc gcggccctca gggcacagcc caaggctgtc agcctcccgg cccagtgatt
      121 tgccttcaag gaaactgggg agtcagaaaa ttgggaactc atatcaacat ggcaaaccta
      181 ttgaaaacag tggtgactgg ctgctcgtgt cctctactta gcaatttggg gtcctgtaag
      241 ggtctacgtg tgaagaagga ttttttacga acattttata ctcaccaaga actgtggtgt
      301 aaagcgcctg taaaaccagg tgcgagcccc tatggttaat ccaacattag gtgttcatga
      361 agctgacctt ttaaagacat caggaacgct gattagtttt atttacccag cccaaaatcc
      421 agagttgcta aataaacttt cccaaagaaa aactacagtt ctggcaatgg accaggttcc
      481 aagagtcaca attgctcagg gatatgatgc gctaagctcc atggccaaca ttgcgggtta
      541 taaggctgtt gtcctagcag caaatcattt tggacgtttt tttactggtc agatcacagc
      601 tgctggaaaa gttcctccag ctaagattct gatagttggt ggtggtgttg ctgggcttgc
      661 ttctgcaggc gcagcaaagt cgatgggtgc aattgttcga ggatttgaca caagagctgc
      721 agctttggaa cagttcaagt ctcttggtgc tgagcccttg gaggtggact tgaaggaatc
      781 tggtgaggga caaggaggat atgcaaaaga gatgtccaaa gagttcattg aagctgaaat
      841 gaaactcttt gctcaacaat gcaaggaggt agacatcctt atcagcacag cacttattcc
      901 aggtaaaaaa gctccagttt tatttaataa agaaatgatt gagtcaatga aggaaggttc
      961 agttgttgtg gatttagctg ctgaggctgg tggaaacttt gaaaccacta agccaggaga
     1021 actctacatt cataagggaa ttactcacat aggctacaca gacctgccca gccgaatggc
     1081 cactcaggcc agcaccctat attccaacaa catcaccaaa ctcctgaagg ccatcagccc
     1141 ggacaaagat aatttttatt ttgatgtgaa agatgacttt gactttggta cgatgggtca
     1201 tgtcattaga ggaactgtag tgatgaaaga tggtaaagtg attttcccag ctcccacacc
     1261 gaaaaatatt cctcaaggtg ccccagtaaa acagaagaca gtggctgagc tggaagctga
     1321 aaaagcagct accattacac ccttcaggaa gacaatgtca acggcttctg catatacagc
     1381 aggtctcaca gggatactgg gtttgggcat tgcggctccc aatctagcct tttctcagat
     1441 ggtgaccact tttggcttgg ctggcattgt ggggtatcat accgtctggg gagtgacccc
     1501 tgctctccac tcaccactga tgtctgtgac aaatgcaatc tcagggctga ctgcagttgg
     1561 tgggttggca ctgatgggag gacatttgta tccttccaca acttctcagg gccttgctgc
     1621 tcttgctgca ttcatatcct ctgtcaacat tgcaggtggc tttctggtga ctcagagaat
     1681 gctggacatg ttcaagcgtc ccactgaccc cccagaatac aactacctgt acctgctccc
     1741 tgccggcacc tttgttggtg gatatttagc tgccctctac agtggttata acattgaaca
     1801 gatcatgtac ctaggctcgg gtttgtgctg tgtcggtgcc ttggctggcc tctccaccca
     1861 gggaacagca cgtcttggca atgcactggg catgattggg gttgctggag gactggcagc
     1921 caccctcgga gtcctaaaac cgggcccaga attactagct cagatgtctg gagcgatggc
     1981 tttgggtggt accattggat tgacaattgc caaacgcatc cagatttctg atttacctca
     2041 attagttgct gcttttcaca gtttagtggg tttggcagct gtacttactt gcatagctga
     2101 gtacattata gaatatccac attttgctac ggatgcagca gcaaatctca ccaagattgt
     2161 ggcctacctc ggcacttaca ttggtggcgt cacctttagt gggtctctca ttgcctatgg
     2221 aaaattgcag ggtctcctga aatctgcccc tctcctactg cctggaaggc acttactcaa
     2281 tgcaggctta ctggctgcta gtgtgggcgg gataatccca ttcatggtgg acccaagctt
     2341 tactactggc atcacctgtc tgggttcagt gtctgctctc tctgctgtca tgggtgtgac
     2401 tttgacagct gctattgggg gtgctgacat gcccgtcgtt atcactgtgc tgaacagcta
     2461 ctcaggctgg gccctgtgtg cagagggctt cctgctcaac aacaatctgc tgaccatcgt
     2521 gggtgcactc ataggctcgt ctggtgctat cctgtcatac atcatgtgtg tggcaatgaa
     2581 tcgctccctg gctaatgtga ttcttggagg ctatggcacc acttcaacag ctggtggaaa
     2641 acccatggaa atttctggca cacatacgga aatcaacctt gacaatgcaa ttgacatgat
     2701 tcgagaagct aatagcatta ttattacacc aggctatggt ctctgtgcag ccaaagctca
     2761 ataccccatt gctgatttgg taaagatgct cactgagcaa ggcaaaaaag tcaggtttgg
     2821 aattcaccca gttgcaggcc gaatgcctgg tcagcttaat gtgctgctgg ctgaggctgg
     2881 tgtgccatat gacattgtgt tggaaatgga tgagatcaac catgattttc cagatactga
     2941 tttggtcctt gtaattggag ctaatgacac tgttaattca gcagctcaag aagatcccaa
     3001 ctctattatt gcaggcatgc cagtccttga ggtctggaaa tcaaagcagg tgattgttat
     3061 gaagaggtct ttgggtgttg gctatgctgc agtggacaat ccaatcttct acaaacctaa
     3121 cacggccatg cttctaggtg atgccaagaa aacatgtgac gcgctccagg cgaaagttag
     3181 agaatcctat cagaagtaaa tattaaggat caagctgtta gctaataatg ccacctctgc
     3241 agttttggga acaggcaaat aaagtatcag tatacatggt gatgtacatc tgtagcaaag
     3301 ctcttggaga aaatgaagac tgaagaaagc aaagcaaaaa ctgtatagag agatttttca
     3361 aaagcagtaa tccctcaatt ttaaaaaagg attgaaaatt ctaaatgtct ttctgtgcat
     3421 attttttgtg ttaggaatca aaagtatttt ataaaaggag aaagaacagc ctcattttag
     3481 atgtagtcct gttggatttt ttatgcctcc tcagtaacca gaaatgtttt aaaaaactaa
     3541 gtgtttagga tttcaagaca acattataca tggctctgaa atatctgaca caatgtaaac
     3601 attgcaggca cctgcatttt atgttttttt tttcaacaaa tgtgactaat ttgaaacttt
     3661 tatgaacttc tgagctgtcc ccttgcaatt caaccgcagt ttgaattaat catatcaaat
     3721 cagttttaat tttttaaatt gtacttcaga gtctatattt caagggcaca ttttctcact
     3781 actattttaa tacattaaag gactaaataa tctttcagag atgctggaaa caaatcattt
     3841 gctttatatg tttcattaga ataccaatga aacatacaac ttgaaaatta gtaatagtat
     3901 ttttgaagat cccatttcta attggagatc tctttaattt cgatcaactt ataatgtgta
     3961 gtactatatt aagtgcactt gagtggaatt caacatttga ctaataaaat gagttcatca
     4021 tgttggcaag tgatgtggca attatctctg gtgacaaaag agtaaaatca aatatttctg
     4081 cctgttacaa atatcaagga agacctgcta ctatgaaata gatgacatta atctgtcttc
     4141 actgtttata atacggatgg attttttttc aaatcagtgt gtgttttgag gtcttatgta
     4201 attgatgaca tttgagagaa atggtggctt tttttagcta cctctttgtt catttaagca
     4261 ccagtaaaga tcatgtcttt ttatagaagt gtagattttc tttgtgactt tgctatcgtg
     4321 cctaaagctc taaatatagg tgaatgtgtg atgaatactc agattatttg tctctctata
     4381 taattagttt ggtactaagt ttctcaaaaa attattaaca catgaaagac aatctctaaa
     4441 ccagaaaaag aagtagtaca aattttgtta ctgtaatgct cgcgtttagt gagtttaaaa
     4501 cacacagtat cttttggttt tataatcagt ttctattttg ctgtgcctga gattaagatc
     4561 tgtgtatgtg tgtgtgtgtg tgtgtgcgtt tgtgtgttaa agcagaaaag acttttttaa
     4621 aagttttaag tgataaatgc aatttgttaa ttgatcttag atcactagta aactcagggc
     4681 tgaattatac catgtatatt ctattagaag aaagtaaaca ccatctttat tcctgccctt
     4741 tttcttctct caaagtagtt gtagttatat ctagaaagaa gcaattttga tttcttgaaa
     4801 aggtagttcc tgcactcagt ttaaactaaa aataatcata cttggatttt atttattttt
     4861 gtcatagtaa aaattttaat ttatatatat ttttatttag tattatctta ttctttgcta
     4921 tttgccaatc ctttgtcatc aattgtgtta aatgaattga aaattcatgc cctgttcatt
     4981 ttattttact ttattggtta ggatatttaa aggatttttg tatatataat ttcttaaatt
     5041 aatattccaa aaggttagtg gacttagatt ataaattatg gcaaaaatct aaaaacaaca
     5101 aaaatgattt ttatacattc tatttcatta ttcctctttt tccaataagt catacaattg
     5161 gtagatatga cttattttat ttttgtatta ttcactatat ctttatgata tttaagtata
     5221 aataattaaa aaaatttatt gtaccttata gtctgtcacc aaaaaaaaaa aattatctgt
     5281 aggtagtgaa atgctaatgt tgatttgtct ttaagggctt gttaactatc ctttattttc
     5341 tcatttgtct taaattagga gtttgtgttt aaattactca tctaagcaaa aaatgtatat
     5401 aaatcccatt actgggtata tacccaaagg attataaatc atgctgctat aaagacacat
     5461 gcacacgtat gtttattgca gcactattca caatagcaaa gacttggaac caacccaaat
     5521 gtccatcaat gatagacttg attaagaaaa tgtgcacata tacaccatgg aatactatgc
     5581 agccataaaa aaggatgagt tcatgtcctt tgtagggaca tggataaagc tggaaaccat
     5641 cattctgagc aaactattgc aaggacagaa aaccaaacac tgcatgttct cactcatagg
     5701 tgggaattga acaatgagaa cacttggaca caaggtgggg aacaccacac accagggcct
     5761 gtcatggggt ggggggagtg gggagggata gcattaggag atatacctaa tgtaaatgat
     5821 gagttaatgg gtgcagcaca ccaacatggc acatgtatac atatgtagca aacctgcacg
     5881 ttgtgcacat gtaccctaga acttaaagta taattaaaaa aaaaaagaaa acagaagcta
     5941 tttataaaga agttatttgc tgaaataaat gtgatctttc ccattaaaaa aataaagaaa
     6001 ttttggggta aaaaaacaca atatattgta ttcttgaaaa attctaagag agtggatgtg
     6061 aagtgttctc accacaaaag tgataactaa ttgaggtaat gcacatatta attagaaaga
     6121 ttttgtcatt ccacaatgta tatatactta aaaatatgtt atacacaata aatacataca
     6181 ttaaaaaata a
//
