LOCUS       NM_006169               2012 bp    mRNA    linear   PRI 03-SEP-2024
DEFINITION  Homo sapiens nicotinamide N-methyltransferase (NNMT), transcript
            variant 1, mRNA.
ACCESSION   NM_006169
VERSION     NM_006169.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Wang,X., Zhao,H., Luo,X., Chen,Y., Shi,C., Wang,Y., Bai,J., Shao,Z.
            and Shang,Z.
  TITLE     NNMT switches the proangiogenic phenotype of cancer-associated
            fibroblasts via epigenetically regulating ETS2/VEGFA axis
  JOURNAL   Oncogene 43 (35), 2647-2660 (2024)
   PUBMED   39069579
  REMARK    GeneRIF: NNMT switches the proangiogenic phenotype of
            cancer-associated fibroblasts via epigenetically regulating
            ETS2/VEGFA axis.
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Ma,Y., Huang,X., Wang,Y., Lei,Y., Yu,J., Yu,S., Gao,Y., Yang,J.,
            Zhao,F., Yu,H., Zeng,J., Chu,Y., Yang,M., Li,G., Xie,X. and
            Zhang,J.
  TITLE     NNMT/1-MNA Promote Cell-Cycle Progression of Breast Cancer by
            Targeting UBC12/Cullin-1-Mediated Degradation of P27 Proteins
  JOURNAL   Adv Sci (Weinh) 11 (9), e2305907 (2024)
   PUBMED   38126621
  REMARK    GeneRIF: NNMT/1-MNA Promote Cell-Cycle Progression of Breast Cancer
            by Targeting UBC12/Cullin-1-Mediated Degradation of P27 Proteins.
REFERENCE   3  (bases 1 to 2012)
  AUTHORS   Lu,J., Gui,W., Tang,S., Shi,Q., Wang,X., Huang,L., Shen,Y.,
            Yang,S., Xiang,J., Yuan,J., Mo,J., Kong,X., Huang,M., Li,X. and
            Lu,C.
  TITLE     Nicotinamide N-Methyltransferase (NNMT) is Involved in Gastric
            Adenocarcinoma Immune Infiltration by Driving Amino Acid Metabolism
  JOURNAL   Nutr Cancer 76 (8), 745-759 (2024)
   PUBMED   38855943
  REMARK    GeneRIF: Nicotinamide N-Methyltransferase (NNMT) is Involved in
            Gastric Adenocarcinoma Immune Infiltration by Driving Amino Acid
            Metabolism.
REFERENCE   4  (bases 1 to 2012)
  AUTHORS   Wen,Q., Xie,X., Chen,C., Wen,B., Liu,Y., Zhou,J., Lin,X., Jin,H.
            and Shi,K.
  TITLE     Lipid reprogramming induced by the NNMT-ABCA1 axis enhanced
            membrane fluidity to promote endometrial cancer progression
  JOURNAL   Aging (Albany NY) 15 (21), 11860-11874 (2023)
   PUBMED   37889548
  REMARK    GeneRIF: Lipid reprogramming induced by the NNMT-ABCA1 axis
            enhanced membrane fluidity to promote endometrial cancer
            progression.
REFERENCE   5  (bases 1 to 2012)
  AUTHORS   Davalieva,K., Kiprijanovska,S., Ivanovski,O., Trifunovski,A.,
            Saidi,S., Dimovski,A. and Popov,Z.
  TITLE     Proteomics Profiling of Bladder Cancer Tissues from Early to
            Advanced Stages Reveals NNMT and GALK1 as Biomarkers for Early
            Detection and Prognosis of BCa
  JOURNAL   Int J Mol Sci 24 (19), 14938 (2023)
   PUBMED   37834386
  REMARK    GeneRIF: Proteomics Profiling of Bladder Cancer Tissues from Early
            to Advanced Stages Reveals NNMT and GALK1 as Biomarkers for Early
            Detection and Prognosis of BCa.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2012)
  AUTHORS   Smith,M.L., Burnett,D., Bennett,P., Waring,R.H., Brown,H.M.,
            Williams,A.C. and Ramsden,D.B.
  TITLE     A direct correlation between nicotinamide N-methyltransferase
            activity and protein levels in human liver cytosol
  JOURNAL   Biochim Biophys Acta 1442 (2-3), 238-244 (1998)
   PUBMED   9804963
REFERENCE   7  (bases 1 to 2012)
  AUTHORS   Scheller,T., Orgacka,H., Szumlanski,C.L. and Weinshilboum,R.M.
  TITLE     Mouse liver nicotinamide N-methyltransferase pharmacogenetics:
            biochemical properties and variation in activity among inbred
            strains
  JOURNAL   Pharmacogenetics 6 (1), 43-53 (1996)
   PUBMED   8845860
REFERENCE   8  (bases 1 to 2012)
  AUTHORS   Aksoy,S., Brandriff,B.F., Ward,A., Little,P.F. and
            Weinshilboum,R.M.
  TITLE     Human nicotinamide N-methyltransferase gene: molecular cloning,
            structural characterization and chromosomal localization
  JOURNAL   Genomics 29 (3), 555-561 (1995)
   PUBMED   8575745
REFERENCE   9  (bases 1 to 2012)
  AUTHORS   Aksoy,S., Szumlanski,C.L. and Weinshilboum,R.M.
  TITLE     Human liver nicotinamide N-methyltransferase. cDNA cloning,
            expression, and biochemical characterization
  JOURNAL   J Biol Chem 269 (20), 14835-14840 (1994)
   PUBMED   8182091
REFERENCE   10 (bases 1 to 2012)
  AUTHORS   Sano,A., Endo,N. and Takitani,S.
  TITLE     Fluorometric assay of rat tissue N-methyltransferases with
            nicotinamide and four isomeric methylnicotinamides
  JOURNAL   Chem Pharm Bull (Tokyo) 40 (1), 153-156 (1992)
   PUBMED   1533573
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AP002518.3.
            
            On Aug 5, 2019 this sequence version replaced NM_006169.2.
            
            Summary: N-methylation is one method by which drug and other
            xenobiotic compounds are metabolized by the liver. This gene
            encodes the protein responsible for this enzymatic activity which
            uses S-adenosyl methionine as the methyl donor. [provided by
            RefSeq, Jul 2008].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803617.149884.1,
                                           SRR1803611.197174.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000299964.4/ ENSP00000299964.3
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-312               AP002518.3         90165-90476
            313-520             AP002518.3         91717-91924
            521-2012            AP002518.3         105811-107302
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="11"
                     /map="11q23.2"
     gene            1..2012
                     /gene="NNMT"
                     /note="nicotinamide N-methyltransferase"
                     /db_xref="GeneID:4837"
                     /db_xref="HGNC:HGNC:7861"
                     /db_xref="MIM:600008"
     exon            1..312
                     /gene="NNMT"
                     /inference="alignment:Splign:2.1.0"
     CDS             159..953
                     /gene="NNMT"
                     /EC_number="2.1.1.1"
                     /codon_start=1
                     /product="nicotinamide N-methyltransferase"
                     /protein_id="NP_006160.1"
                     /db_xref="CCDS:CCDS8368.1"
                     /db_xref="GeneID:4837"
                     /db_xref="HGNC:HGNC:7861"
                     /db_xref="MIM:600008"
                     /translation="MESGFTSKDTYLSHFNPRDYLEKYYKFGSRHSAESQILKHLLKN
                     LFKIFCLDGVKGDLLIDIGSGPTIYQLLSACESFKEIVVTDYSDQNLQELEKWLKKEP
                     EAFDWSPVVTYVCDLEGNRVKGPEKEEKLRQAVKQVLKCDVTQSQPLGAVPLPPADCV
                     LSTLCLDAACPDLPTYCRALRNLGSLLKPGGFLVIMDALKSSYYMIGEQKFSSLPLGR
                     EAVEAAVKEAGYTIEWFEVISQSYSSTMANNEGLFSLVARKLSRPL"
     exon            313..520
                     /gene="NNMT"
                     /inference="alignment:Splign:2.1.0"
     exon            521..2012
                     /gene="NNMT"
                     /inference="alignment:Splign:2.1.0"
     regulatory      968..973
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NNMT"
                     /note="hexamer: ATTAAA"
     polyA_site      994
                     /gene="NNMT"
                     /note="major polyA site"
     regulatory      1971..1976
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NNMT"
                     /note="hexamer: AATAAT"
     regulatory      1988..1993
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NNMT"
                     /note="hexamer: AATAAA"
     polyA_site      1989
                     /gene="NNMT"
     polyA_site      2012
                     /gene="NNMT"
ORIGIN      
        1 agtgtttaac caaccatctg ttctaaaaga agggctgaac tgatggaagg aatgctgtta
       61 gcctgagact caggaagaca acttctgcag ggtcactccc tggcttctgg aggaaagaga
      121 aggagggcag tgctccagtg gtacagaagt gagacataat ggaatcaggc ttcacctcca
      181 aggacaccta tctaagccat tttaaccctc gggattacct agaaaaatat tacaagtttg
      241 gttctaggca ctctgcagaa agccagattc ttaagcacct tctgaaaaat cttttcaaga
      301 tattctgcct agacggtgtg aagggagacc tgctgattga catcggctct ggccccacta
      361 tctatcagct cctctctgct tgtgaatcct ttaaggagat cgtcgtcact gactactcag
      421 accagaacct gcaggagctg gagaagtggc tgaagaaaga gccagaggcc tttgactggt
      481 ccccagtggt gacctatgtg tgtgatcttg aagggaacag agtcaagggt ccagagaagg
      541 aggagaagtt gagacaggcg gtcaagcagg tgctgaagtg tgatgtgact cagagccagc
      601 cactgggggc cgtcccctta cccccggctg actgcgtgct cagcacactg tgtctggatg
      661 ccgcctgccc agacctcccc acctactgca gggcgctcag gaacctcggc agcctactga
      721 agccaggggg cttcctggtg atcatggatg cgctcaagag cagctactac atgattggtg
      781 agcagaagtt ctccagcctc cccctgggcc gggaggcagt agaggctgct gtgaaagagg
      841 ctggctacac aatcgaatgg tttgaggtga tctcgcaaag ttattcttcc accatggcca
      901 acaacgaagg acttttctcc ctggtggcga ggaagctgag cagacccctg tgatgcctgt
      961 gacctcaatt aaagcaattc ctttgacctg tccagttgac tttagtcctt gtttctaact
     1021 gccaagtcat gtgctgagta gaggctcagt ggttggggcc caatggttca tctaggacgg
     1081 gactagagag gtcagtctac aagcaatcca ttgaccactt acttggtgct gcacacaaat
     1141 gttggtgcta tgggacccaa agatgagcaa ttagtattcc agtcttcatt gcctgtgctt
     1201 acaaaagaag acctcacttc cctaaacatc tagttatggc ggctcaagcc cgtacctgcc
     1261 tacagagaag tgtctgcagt tactcactat tagtttccta agggggcact gctggctcct
     1321 tctctcccag gaatgggctt ctcctattct ttaactcttg ccttctccct ggagaggcta
     1381 ccaaaccctg gcctctgccg ctgttcaggc tgccatgtgg gtatagctgt ttgctagact
     1441 ccactctggc ctcaggggcc agagaacagc tgtccagcat ttctgttcat cccagcaatc
     1501 caaaaaaaaa tttttttaag ctacaaagat cactccattt tcaaattcag actctgccac
     1561 ttattagctg tttctcatta ggcaagttat ataaccttgc agtgcctcac tttcctcatc
     1621 tataaaatga gattaaaata gtctttttat taagagaatt aaagtgaatt gtggccaggc
     1681 actgtggctc atgcctgtaa tcccagcgct ttgggaggct gaggcagaca gattgcttga
     1741 gctcagaagt tcgagactgg cctggggaac atgcaaaaac cctgtctgta ttaaaaatac
     1801 aaaaaaatta gctaggtgtg gcggcatatg cctgtggtcc cagctactca ggaggctgaa
     1861 gtgggaggat ggcttgagcc cgggaggcgg aggttgcaat gaactgagat cgcaccactg
     1921 tgttccagac agagtgcgat gctgtctcga aaaaagtaaa ataataaata aataataaat
     1981 aaaataaaat aaagtgaatt gttacatgta aa
//
