LOCUS       NM_002512                690 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens NME/NM23 nucleoside diphosphate kinase 2 (NME2),
            transcript variant 1, mRNA.
ACCESSION   NM_002512
VERSION     NM_002512.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 690)
  AUTHORS   Zhang,S., Nelson,O.D., Price,I.R., Zhu,C., Lu,X., Fernandez,I.R.,
            Weiss,R.S. and Lin,H.
  TITLE     Long-chain fatty acyl coenzyme A inhibits NME1/2 and regulates
            cancer metastasis
  JOURNAL   Proc Natl Acad Sci U S A 119 (11), e2117013119 (2022)
   PUBMED   35259022
  REMARK    GeneRIF: Long-chain fatty acyl coenzyme A inhibits NME1/2 and
            regulates cancer metastasis.
REFERENCE   2  (bases 1 to 690)
  AUTHORS   Qi,Y., Wei,J. and Zhang,X.
  TITLE     Requirement of transcription factor NME2 for the maintenance of the
            stemness of gastric cancer stem-like cells
  JOURNAL   Cell Death Dis 12 (10), 924 (2021)
   PUBMED   34628473
  REMARK    GeneRIF: Requirement of transcription factor NME2 for the
            maintenance of the stemness of gastric cancer stem-like cells.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 690)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 690)
  AUTHORS   Chatterjee,A., Eshwaran,R., Poschet,G., Lomada,S., Halawa,M.,
            Wilhelm,K., Schmidt,M., Hammes,H.P., Wieland,T. and Feng,Y.
  TITLE     Involvement of NDPK-B in Glucose Metabolism-Mediated Endothelial
            Damage via Activation of the Hexosamine Biosynthesis Pathway and
            Suppression of O-GlcNAcase Activity
  JOURNAL   Cells 9 (10), 2324 (2020)
   PUBMED   33086728
  REMARK    GeneRIF: Involvement of NDPK-B in Glucose Metabolism-Mediated
            Endothelial Damage via Activation of the Hexosamine Biosynthesis
            Pathway and Suppression of O-GlcNAcase Activity.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 690)
  AUTHORS   Sengupta,P. and Chatterjee,S.
  TITLE     Inosine 5'-diphosphate, a molecular decoy rescues Nucleoside
            diphosphate kinase from c-MYC G-Quadruplex unfolding
  JOURNAL   Biochim Biophys Acta Gen Subj 1864 (9), 129649 (2020)
   PUBMED   32492501
  REMARK    GeneRIF: Inosine 5'-diphosphate, a molecular decoy rescues
            Nucleoside diphosphate kinase from c-MYC G-Quadruplex unfolding.
REFERENCE   6  (bases 1 to 690)
  AUTHORS   Seifert,M., Seib,T., Engel,M., Dooley,S. and Welter,C.
  TITLE     Characterization of the human nm23-H2 promoter region and
            localization of the microsatellite D17S396
  JOURNAL   Biochem Biophys Res Commun 215 (3), 910-914 (1995)
   PUBMED   7488060
REFERENCE   7  (bases 1 to 690)
  AUTHORS   Webb,P.A., Perisic,O., Mendola,C.E., Backer,J.M. and Williams,R.L.
  TITLE     The crystal structure of a human nucleoside diphosphate kinase,
            NM23-H2
  JOURNAL   J Mol Biol 251 (4), 574-587 (1995)
   PUBMED   7658474
REFERENCE   8  (bases 1 to 690)
  AUTHORS   Gilles,A.M., Presecan,E., Vonica,A. and Lascu,I.
  TITLE     Nucleoside diphosphate kinase from human erythrocytes. Structural
            characterization of the two polypeptide chains responsible for
            heterogeneity of the hexameric enzyme
  JOURNAL   J Biol Chem 266 (14), 8784-8789 (1991)
   PUBMED   1851158
REFERENCE   9  (bases 1 to 690)
  AUTHORS   Stahl,J.A., Leone,A., Rosengard,A.M., Porter,L., King,C.R. and
            Steeg,P.S.
  TITLE     Identification of a second human nm23 gene, nm23-H2
  JOURNAL   Cancer Res 51 (1), 445-449 (1991)
   PUBMED   1988104
REFERENCE   10 (bases 1 to 690)
  AUTHORS   Rosengard,A.M., Krutzsch,H.C., Shearn,A., Biggs,J.R., Barker,E.,
            Margulies,I.M., King,C.R., Liotta,L.A. and Steeg,P.S.
  TITLE     Reduced Nm23/Awd protein in tumour metastasis and aberrant
            Drosophila development
  JOURNAL   Nature 342 (6246), 177-180 (1989)
   PUBMED   2509941
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. This record has been curated by NCBI staff in
            collaboration with Francesco Amaldi. The reference sequence was
            derived from DB454073.1, BF034471.1 and BC095458.1.
            
            On Jun 2, 2019 this sequence version replaced NM_002512.3.
            
            Summary: Nucleoside diphosphate kinase (NDK) exists as a hexamer
            composed of 'A' (encoded by NME1) and 'B' (encoded by this gene)
            isoforms. Multiple alternatively spliced transcript variants have
            been found for this gene. Read-through transcription from the
            neighboring upstream gene (NME1) generates naturally-occurring
            transcripts (NME1-NME2) that encode a fusion protein comprised of
            sequence sharing identity with each individual gene product.
            [provided by RefSeq, Nov 2010].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (a). Variants 1-4 encode the same isoform.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BG707449.1, SRR5189661.36994.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000512737.6/ ENSP00000421064.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-32                DB454073.1         140-171
            33-50               BF034471.1         96-113
            51-690              BC095458.1         1-640
FEATURES             Location/Qualifiers
     source          1..690
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q21.33"
     gene            1..690
                     /gene="NME2"
                     /gene_synonym="NDKB; NDPK-B; NDPKB; NM23-H2; NM23B; PUF"
                     /note="NME/NM23 nucleoside diphosphate kinase 2"
                     /db_xref="GeneID:4831"
                     /db_xref="HGNC:HGNC:7850"
                     /db_xref="MIM:156491"
     exon            1..87
                     /gene="NME2"
                     /gene_synonym="NDKB; NDPK-B; NDPKB; NM23-H2; NM23B; PUF"
                     /inference="alignment:Splign:2.1.0"
     exon            88..217
                     /gene="NME2"
                     /gene_synonym="NDKB; NDPK-B; NDPKB; NM23-H2; NM23B; PUF"
                     /inference="alignment:Splign:2.1.0"
     CDS             92..550
                     /gene="NME2"
                     /gene_synonym="NDKB; NDPK-B; NDPKB; NM23-H2; NM23B; PUF"
                     /EC_number="2.7.4.6"
                     /EC_number="2.7.13.3"
                     /note="isoform a is encoded by transcript variant 1; NDP
                     kinase B; c-myc transcription factor; non-metastatic cells
                     2, protein (NM23) expressed in; c-myc purine-binding
                     transcription factor PUF; nucleoside diphosphate kinase B;
                     histidine protein kinase NDKB; nucleotide diphosphate
                     kinase B; non-metastatic cells 2, protein (NM23B)
                     expressed in; HEL-S-155an; epididymis secretory sperm
                     binding protein Li 155an"
                     /codon_start=1
                     /product="nucleoside diphosphate kinase B isoform a"
                     /protein_id="NP_002503.1"
                     /db_xref="CCDS:CCDS11580.1"
                     /db_xref="GeneID:4831"
                     /db_xref="HGNC:HGNC:7850"
                     /db_xref="MIM:156491"
                     /translation="MANLERTFIAIKPDGVQRGLVGEIIKRFEQKGFRLVAMKFLRAS
                     EEHLKQHYIDLKDRPFFPGLVKYMNSGPVVAMVWEGLNVVKTGRVMLGETNPADSKPG
                     TIRGDFCIQVGRNIIHGSDSVKSAEKEISLWFKPEELVDYKSCAHDWVYE"
     exon            218..319
                     /gene="NME2"
                     /gene_synonym="NDKB; NDPK-B; NDPKB; NM23-H2; NM23B; PUF"
                     /inference="alignment:Splign:2.1.0"
     exon            320..432
                     /gene="NME2"
                     /gene_synonym="NDKB; NDPK-B; NDPKB; NM23-H2; NM23B; PUF"
                     /inference="alignment:Splign:2.1.0"
     exon            433..690
                     /gene="NME2"
                     /gene_synonym="NDKB; NDPK-B; NDPKB; NM23-H2; NM23B; PUF"
                     /inference="alignment:Splign:2.1.0"
     regulatory      669..674
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NME2"
                     /gene_synonym="NDKB; NDPK-B; NDPKB; NM23-H2; NM23B; PUF"
                     /note="hexamer: AATAAA"
     polyA_site      690
                     /gene="NME2"
                     /gene_synonym="NDKB; NDPK-B; NDPKB; NM23-H2; NM23B; PUF"
                     /note="major polyA site"
ORIGIN      
        1 ctccgccgcc ggctcccggg tgtggtggtc gcaccagctc tctgctctcc cagcgcagcg
       61 ccgccgcccg gcccctccag cttcccggac catggccaac ctggagcgca ccttcatcgc
      121 catcaagccg gacggcgtgc agcgcggcct ggtgggcgag atcatcaagc gcttcgagca
      181 gaagggattc cgcctcgtgg ccatgaagtt cctccgggcc tctgaagaac acctgaagca
      241 gcactacatt gacctgaaag accgaccatt cttccctggg ctggtgaagt acatgaactc
      301 agggccggtt gtggccatgg tctgggaggg gctgaacgtg gtgaagacag gccgagtgat
      361 gcttggggag accaatccag cagattcaaa gccaggcacc attcgtgggg acttctgcat
      421 tcaggttggc aggaacatca ttcatggcag tgattcagta aaaagtgctg aaaaagaaat
      481 cagcctatgg tttaagcctg aagaactggt tgactacaag tcttgtgctc atgactgggt
      541 ctatgaataa gaggtggaca caacagcagt ctccttcagc acggcgtggt gtgtccctgg
      601 acacagctct tcattccatt gacttagagg caacaggatt gatcattctt ttatagagca
      661 tatttgccaa taaagctttt ggaagccgga
//
