LOCUS       NM_033004               5610 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens NLR family pyrin domain containing 1 (NLRP1),
            transcript variant 1, mRNA.
ACCESSION   NM_033004 NM_021730
VERSION     NM_033004.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5610)
  AUTHORS   Geeson,M.B., Hsiao,J.C., Tsamouri,L.P., Ball,D.P. and
            Bachovchin,D.A.
  TITLE     The interaction between NLRP1 and oxidized TRX1 involves a
            transient disulfide bond
  JOURNAL   Cell Chem Biol 31 (5), 955-961 (2024)
   PUBMED   38215746
  REMARK    GeneRIF: The interaction between NLRP1 and oxidized TRX1 involves a
            transient disulfide bond.
REFERENCE   2  (bases 1 to 5610)
  AUTHORS   He,H., Li,Y., Chen,Y., Chen,J., Li,Z., Li,L., Shi,D., Zhang,X.,
            Shi,H., Xue,M. and Feng,L.
  TITLE     NLRP1 restricts porcine deltacoronavirus infection via IL-11
            inhibiting the phosphorylation of the ERK signaling pathway
  JOURNAL   J Virol 98 (3), e0198223 (2024)
   PUBMED   38411106
  REMARK    GeneRIF: NLRP1 restricts porcine deltacoronavirus infection via
            IL-11 inhibiting the phosphorylation of the ERK signaling pathway.
REFERENCE   3  (bases 1 to 5610)
  AUTHORS   Spanic Popovacki,E., Vogrinc,D., Fuller,H.R., Langer Horvat,L.,
            Mayer,D., Kopic,J., Pintaric,K., Babic Leko,M., Pravica,M.,
            Krsnik,Z., Marcinko,D., Sagud,M., Hof,P.R., Mladinov,M. and
            Simic,G.
  TITLE     Increased NLRP1 mRNA and Protein Expression Suggests Inflammasome
            Activation in the Dorsolateral Prefrontal and Medial Orbitofrontal
            Cortex in Schizophrenia
  JOURNAL   Biomolecules 14 (3), 302 (2024)
   PUBMED   38540722
  REMARK    GeneRIF: Increased NLRP1 mRNA and Protein Expression Suggests
            Inflammasome Activation in the Dorsolateral Prefrontal and Medial
            Orbitofrontal Cortex in Schizophrenia.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5610)
  AUTHORS   Calabrese,L., Fiocco,Z., Mellett,M., Aoki,R., Rubegni,P.,
            French,L.E. and Satoh,T.K.
  TITLE     Role of the NLRP1 inflammasome in skin cancer and inflammatory skin
            diseases
  JOURNAL   Br J Dermatol 190 (3), 305-315 (2024)
   PUBMED   37889986
  REMARK    GeneRIF: Role of the NLRP1 inflammasome in skin cancer and
            inflammatory skin diseases.
            Review article
REFERENCE   5  (bases 1 to 5610)
  AUTHORS   Barry,K., Murphy,C. and Mansell,A.
  TITLE     NLRP1- A CINDERELLA STORY: a perspective of recent advances in
            NLRP1 and the questions they raise
  JOURNAL   Commun Biol 6 (1), 1274 (2023)
   PUBMED   38104185
  REMARK    GeneRIF: NLRP1- A CINDERELLA STORY: a perspective of recent
            advances in NLRP1 and the questions they raise.
            Review article
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5610)
  AUTHORS   Chu,Z.L., Pio,F., Xie,Z., Welsh,K., Krajewska,M., Krajewski,S.,
            Godzik,A. and Reed,J.C.
  TITLE     A novel enhancer of the Apaf1 apoptosome involved in cytochrome
            c-dependent caspase activation and apoptosis
  JOURNAL   J Biol Chem 276 (12), 9239-9245 (2001)
   PUBMED   11113115
REFERENCE   7  (bases 1 to 5610)
  AUTHORS   Hlaing,T., Guo,R.F., Dilley,K.A., Loussia,J.M., Morrish,T.A.,
            Shi,M.M., Vincenz,C. and Ward,P.A.
  TITLE     Molecular cloning and characterization of DEFCAP-L and -S, two
            isoforms of a novel member of the mammalian Ced-4 family of
            apoptosis proteins
  JOURNAL   J Biol Chem 276 (12), 9230-9238 (2001)
   PUBMED   11076957
REFERENCE   8  (bases 1 to 5610)
  AUTHORS   Martinon,F., Hofmann,K. and Tschopp,J.
  TITLE     The pyrin domain: a possible member of the death domain-fold family
            implicated in apoptosis and inflammation
  JOURNAL   Curr Biol 11 (4), R118-R120 (2001)
   PUBMED   11250163
REFERENCE   9  (bases 1 to 5610)
  AUTHORS   Thorpe,K.L., Abdulla,S., Kaufman,J., Trowsdale,J. and Beck,S.
  TITLE     Phylogeny and structure of the RING3 gene
  JOURNAL   Immunogenetics 44 (5), 391-396 (1996)
   PUBMED   8781126
REFERENCE   10 (bases 1 to 5610)
  AUTHORS   Gu,Y., Sarnecki,C., Aldape,R.A., Livingston,D.J. and Su,M.S.
  TITLE     Cleavage of poly(ADP-ribose) polymerase by interleukin-1 beta
            converting enzyme and its homologs TX and Nedd-2
  JOURNAL   J Biol Chem 270 (32), 18715-18718 (1995)
   PUBMED   7642516
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC055839.9, AF229060.1,
            AB023143.1 and AI819334.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_033004.3.
            
            Summary: This gene encodes a member of the Ced-4 family of
            apoptosis proteins. Ced-family members contain a caspase
            recruitment domain (CARD) and are known to be key mediators of
            programmed cell death. The encoded protein contains a distinct
            N-terminal pyrin-like motif, which is possibly involved in
            protein-protein interactions. This protein interacts strongly with
            caspase 2 and weakly with caspase 9. Overexpression of this gene
            was demonstrated to induce apoptosis in cells. Multiple
            alternatively spliced transcript variants encoding distinct
            isoforms have been found for this gene, but the biological validity
            of some variants has not been determined. [provided by RefSeq, Jul
            2008].
            
            Transcript Variant: This variant (1) encodes the longest isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR18074968.2449760.1, AF229060.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000572272.6/ ENSP00000460475.1
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-552               AC055839.9         96884-97435         c
            553-4974            AF229060.1         1-4422
            4975-5606           AB023143.1         4813-5444
            5607-5610           AI819334.1         11-14               c
FEATURES             Location/Qualifiers
     source          1..5610
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17p13.2"
     gene            1..5610
                     /gene="NLRP1"
                     /gene_synonym="AIADK; CARD7; CIDED; CLR17.1; DEFCAP;
                     DEFCAP-L/S; JRRP; MSPC; NAC; NALP1; PP1044; SLEV1; VAMAS1"
                     /note="NLR family pyrin domain containing 1"
                     /db_xref="GeneID:22861"
                     /db_xref="HGNC:HGNC:14374"
                     /db_xref="MIM:606636"
     exon            1..823
                     /gene="NLRP1"
                     /gene_synonym="AIADK; CARD7; CIDED; CLR17.1; DEFCAP;
                     DEFCAP-L/S; JRRP; MSPC; NAC; NALP1; PP1044; SLEV1; VAMAS1"
                     /inference="alignment:Splign:2.1.0"
     CDS             553..4974
                     /gene="NLRP1"
                     /gene_synonym="AIADK; CARD7; CIDED; CLR17.1; DEFCAP;
                     DEFCAP-L/S; JRRP; MSPC; NAC; NALP1; PP1044; SLEV1; VAMAS1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     NACHT, leucine rich repeat and PYD containing 1; death
                     effector filament-forming Ced-4-like apoptosis protein;
                     nucleotide-binding oligomerization domain, leucine rich
                     repeat and pyrin domain containing 1; NACHT, leucine rich
                     repeat and PYD (pyrin domain) containing 1; NACHT, LRR and
                     PYD containing protein 1; caspase recruitment domain
                     protein 7; NACHT, LRR and PYD domains-containing protein
                     1; caspase recruitment domain-containing protein 7;
                     nucleotide-binding domain and caspase recruitment domain"
                     /codon_start=1
                     /product="NACHT, LRR and PYD domains-containing protein 1
                     isoform 1"
                     /protein_id="NP_127497.1"
                     /db_xref="CCDS:CCDS42246.1"
                     /db_xref="GeneID:22861"
                     /db_xref="HGNC:HGNC:14374"
                     /db_xref="MIM:606636"
                     /translation="MAGGAWGRLACYLEFLKKEELKEFQLLLANKAHSRSSSGETPAQ
                     PEKTSGMEVASYLVAQYGEQRAWDLALHTWEQMGLRSLCAQAQEGAGHSPSFPYSPSE
                     PHLGSPSQPTSTAVLMPWIHELPAGCTQGSERRVLRQLPDTSGRRWREISASLLYQAL
                     PSSPDHESPSQESPNAPTSTAVLGSWGSPPQPSLAPREQEAPGTQWPLDETSGIYYTE
                     IREREREKSEKGRPPWAAVVGTPPQAHTSLQPHHHPWEPSVRESLCSTWPWKNEDFNQ
                     KFTQLLLLQRPHPRSQDPLVKRSWPDYVEENRGHLIEIRDLFGPGLDTQEPRIVILQG
                     AAGIGKSTLARQVKEAWGRGQLYGDRFQHVFYFSCRELAQSKVVSLAELIGKDGTATP
                     APIRQILSRPERLLFILDGVDEPGWVLQEPSSELCLHWSQPQPADALLGSLLGKTILP
                     EASFLITARTTALQNLIPSLEQARWVEVLGFSESSRKEYFYRYFTDERQAIRAFRLVK
                     SNKELWALCLVPWVSWLACTCLMQQMKRKEKLTLTSKTTTTLCLHYLAQALQAQPLGP
                     QLRDLCSLAAEGIWQKKTLFSPDDLRKHGLDGAIISTFLKMGILQEHPIPLSYSFIHL
                     CFQEFFAAMSYVLEDEKGRGKHSNCIIDLEKTLEAYGIHGLFGASTTRFLLGLLSDEG
                     EREMENIFHCRLSQGRNLMQWVPSLQLLLQPHSLESLHCLYETRNKTFLTQVMAHFEE
                     MGMCVETDMELLVCTFCIKFSRHVKKLQLIEGRQHRSTWSPTMVVLFRWVPVTDAYWQ
                     ILFSVLKVTRNLKELDLSGNSLSHSAVKSLCKTLRRPRCLLETLRLAGCGLTAEDCKD
                     LAFGLRANQTLTELDLSFNVLTDAGAKHLCQRLRQPSCKLQRLQLVSCGLTSDCCQDL
                     ASVLSASPSLKELDLQQNNLDDVGVRLLCEGLRHPACKLIRLGLDQTTLSDEMRQELR
                     ALEQEKPQLLIFSRRKPSVMTPTEGLDTGEMSNSTSSLKRQRLGSERAASHVAQANLK
                     LLDVSKIFPIAEIAEESSPEVVPVELLCVPSPASQGDLHTKPLGTDDDFWGPTGPVAT
                     EVVDKEKNLYRVHFPVAGSYRWPNTGLCFVMREAVTVEIEFCVWDQFLGEINPQHSWM
                     VAGPLLDIKAEPGAVEAVHLPHFVALQGGHVDTSLFQMAHFKEEGMLLEKPARVELHH
                     IVLENPSFSPLGVLLKMIHNALRFIPVTSVVLLYHRVHPEEVTFHLYLIPSDCSIRKA
                     IDDLEMKFQFVRIHKPPPLTPLYMGCRYTVSGSGSGMLEILPKELELCYRSPGEDQLF
                     SEFYVGHLGSGIRLQVKDKKDETLVWEALVKPGDLMPATTLIPPARIAVPSPLDAPQL
                     LHFVDQYREQLIARVTSVEVVLDKLHGQVLSQEQYERVLAENTRPSQMRKLFSLSQSW
                     DRKCKDGLYQALKETHPHLIMELWEKGSKKGLLPLSS"
     exon            824..1000
                     /gene="NLRP1"
                     /gene_synonym="AIADK; CARD7; CIDED; CLR17.1; DEFCAP;
                     DEFCAP-L/S; JRRP; MSPC; NAC; NALP1; PP1044; SLEV1; VAMAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1001..1204
                     /gene="NLRP1"
                     /gene_synonym="AIADK; CARD7; CIDED; CLR17.1; DEFCAP;
                     DEFCAP-L/S; JRRP; MSPC; NAC; NALP1; PP1044; SLEV1; VAMAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            1205..2909
                     /gene="NLRP1"
                     /gene_synonym="AIADK; CARD7; CIDED; CLR17.1; DEFCAP;
                     DEFCAP-L/S; JRRP; MSPC; NAC; NALP1; PP1044; SLEV1; VAMAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            2910..3080
                     /gene="NLRP1"
                     /gene_synonym="AIADK; CARD7; CIDED; CLR17.1; DEFCAP;
                     DEFCAP-L/S; JRRP; MSPC; NAC; NALP1; PP1044; SLEV1; VAMAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            3081..3251
                     /gene="NLRP1"
                     /gene_synonym="AIADK; CARD7; CIDED; CLR17.1; DEFCAP;
                     DEFCAP-L/S; JRRP; MSPC; NAC; NALP1; PP1044; SLEV1; VAMAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            3252..3422
                     /gene="NLRP1"
                     /gene_synonym="AIADK; CARD7; CIDED; CLR17.1; DEFCAP;
                     DEFCAP-L/S; JRRP; MSPC; NAC; NALP1; PP1044; SLEV1; VAMAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            3423..3512
                     /gene="NLRP1"
                     /gene_synonym="AIADK; CARD7; CIDED; CLR17.1; DEFCAP;
                     DEFCAP-L/S; JRRP; MSPC; NAC; NALP1; PP1044; SLEV1; VAMAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            3513..3604
                     /gene="NLRP1"
                     /gene_synonym="AIADK; CARD7; CIDED; CLR17.1; DEFCAP;
                     DEFCAP-L/S; JRRP; MSPC; NAC; NALP1; PP1044; SLEV1; VAMAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            3605..3685
                     /gene="NLRP1"
                     /gene_synonym="AIADK; CARD7; CIDED; CLR17.1; DEFCAP;
                     DEFCAP-L/S; JRRP; MSPC; NAC; NALP1; PP1044; SLEV1; VAMAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            3686..3848
                     /gene="NLRP1"
                     /gene_synonym="AIADK; CARD7; CIDED; CLR17.1; DEFCAP;
                     DEFCAP-L/S; JRRP; MSPC; NAC; NALP1; PP1044; SLEV1; VAMAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            3849..4072
                     /gene="NLRP1"
                     /gene_synonym="AIADK; CARD7; CIDED; CLR17.1; DEFCAP;
                     DEFCAP-L/S; JRRP; MSPC; NAC; NALP1; PP1044; SLEV1; VAMAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            4073..4335
                     /gene="NLRP1"
                     /gene_synonym="AIADK; CARD7; CIDED; CLR17.1; DEFCAP;
                     DEFCAP-L/S; JRRP; MSPC; NAC; NALP1; PP1044; SLEV1; VAMAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            4336..4467
                     /gene="NLRP1"
                     /gene_synonym="AIADK; CARD7; CIDED; CLR17.1; DEFCAP;
                     DEFCAP-L/S; JRRP; MSPC; NAC; NALP1; PP1044; SLEV1; VAMAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            4468..4609
                     /gene="NLRP1"
                     /gene_synonym="AIADK; CARD7; CIDED; CLR17.1; DEFCAP;
                     DEFCAP-L/S; JRRP; MSPC; NAC; NALP1; PP1044; SLEV1; VAMAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            4610..4654
                     /gene="NLRP1"
                     /gene_synonym="AIADK; CARD7; CIDED; CLR17.1; DEFCAP;
                     DEFCAP-L/S; JRRP; MSPC; NAC; NALP1; PP1044; SLEV1; VAMAS1"
                     /inference="alignment:Splign:2.1.0"
     exon            4655..5610
                     /gene="NLRP1"
                     /gene_synonym="AIADK; CARD7; CIDED; CLR17.1; DEFCAP;
                     DEFCAP-L/S; JRRP; MSPC; NAC; NALP1; PP1044; SLEV1; VAMAS1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5451..5456
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NLRP1"
                     /gene_synonym="AIADK; CARD7; CIDED; CLR17.1; DEFCAP;
                     DEFCAP-L/S; JRRP; MSPC; NAC; NALP1; PP1044; SLEV1; VAMAS1"
                     /note="hexamer: AATAAA"
     polyA_site      5479
                     /gene="NLRP1"
                     /gene_synonym="AIADK; CARD7; CIDED; CLR17.1; DEFCAP;
                     DEFCAP-L/S; JRRP; MSPC; NAC; NALP1; PP1044; SLEV1; VAMAS1"
                     /note="major polyA site"
     regulatory      5560..5565
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NLRP1"
                     /gene_synonym="AIADK; CARD7; CIDED; CLR17.1; DEFCAP;
                     DEFCAP-L/S; JRRP; MSPC; NAC; NALP1; PP1044; SLEV1; VAMAS1"
                     /note="hexamer: AATACA"
     polyA_site      5577
                     /gene="NLRP1"
                     /gene_synonym="AIADK; CARD7; CIDED; CLR17.1; DEFCAP;
                     DEFCAP-L/S; JRRP; MSPC; NAC; NALP1; PP1044; SLEV1; VAMAS1"
                     /note="major polyA site"
     regulatory      5582..5587
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NLRP1"
                     /gene_synonym="AIADK; CARD7; CIDED; CLR17.1; DEFCAP;
                     DEFCAP-L/S; JRRP; MSPC; NAC; NALP1; PP1044; SLEV1; VAMAS1"
                     /note="hexamer: AATAAA"
     polyA_site      5610
                     /gene="NLRP1"
                     /gene_synonym="AIADK; CARD7; CIDED; CLR17.1; DEFCAP;
                     DEFCAP-L/S; JRRP; MSPC; NAC; NALP1; PP1044; SLEV1; VAMAS1"
ORIGIN      
        1 gttgactagg cgctgttctt gctggctggt gccccagggc ctggagaggt ctgaagaaac
       61 ctgggagcca gcagcccggg gctccactct gggttctgaa agcccattcc ctgctctgcg
      121 gctcctccca ccccacctct tctcagcctt gcagctcaag ggttgatctc aggagtccag
      181 gacccaggag agggaagaat ctgaggaaca cagaacagtg agcgttgccc acaccccatc
      241 tcccgtcacc acatctcccc tcaccctcac cctccctgcc tggccctgga ccccatccca
      301 ggacctccct atcagctgac ttcttccagt gtcttgcagg cccctctggg ctcctccctc
      361 ccctggcttt tcctaccact ccccctctat cggcgtctat ctgtaggtgc cctgggattt
      421 ataaaactgg gttccgaatg ctgaataaga gacggtaaga gccaaggcaa aggacagcac
      481 tgttctctgc ctgcctgata ccctcaccac ctgggaacat cccccagaca ccctcttaac
      541 tccgggacag agatggctgg cggagcctgg ggccgcctgg cctgttactt ggagttcctg
      601 aagaaggagg agctgaagga gttccagctt ctgctcgcca ataaagcgca ctccaggagc
      661 tcttcgggtg agacacccgc tcagccagag aagacgagtg gcatggaggt ggcctcgtac
      721 ctggtggctc agtatgggga gcagcgggcc tgggacctag ccctccatac ctgggagcag
      781 atggggctga ggtcactgtg cgcccaagcc caggaagggg caggccactc tccctcattc
      841 ccctacagcc caagtgaacc ccacctgggg tctcccagcc aacccacctc caccgcagtg
      901 ctaatgccct ggatccatga attgccggcg gggtgcaccc agggctcaga gagaagggtt
      961 ttgagacagc tgcctgacac atctggacgc cgctggagag aaatctctgc ctcactcctc
     1021 taccaagctc ttccaagctc cccagaccat gagtctccaa gccaggagtc acccaacgcc
     1081 cccacatcca cagcagtgct ggggagctgg ggatccccac ctcagcccag cctagcaccc
     1141 agagagcagg aggctcctgg gacccaatgg cctctggatg aaacgtcagg aatttactac
     1201 acagaaatca gagaaagaga gagagagaaa tcagagaaag gcaggccccc atgggcagcg
     1261 gtggtaggaa cgcccccaca ggcgcacacc agcctacagc cccaccacca cccatgggag
     1321 ccttctgtga gagagagcct ctgttccaca tggccctgga aaaatgagga ttttaaccaa
     1381 aaattcacac agctgctact tctacaaaga cctcacccca gaagccaaga tcccctggtc
     1441 aagagaagct ggcctgatta tgtggaggag aatcgaggac atttaattga gatcagagac
     1501 ttatttggcc caggcctgga tacccaagaa cctcgcatag tcatactgca gggggctgct
     1561 ggaattggga agtcaacact ggccaggcag gtgaaggaag cctgggggag aggccagctg
     1621 tatggggacc gcttccagca tgtcttctac ttcagctgca gagagctggc ccagtccaag
     1681 gtggtgagtc tcgctgagct catcggaaaa gatgggacag ccactccggc tcccattaga
     1741 cagatcctgt ctaggccaga gcggctgctc ttcatcctcg atggtgtaga tgagccagga
     1801 tgggtcttgc aggagccgag ttctgagctc tgtctgcact ggagccagcc acagccggcg
     1861 gatgcactgc tgggcagttt gctggggaaa actatacttc ccgaggcatc cttcctgatc
     1921 acggctcgga ccacagctct gcagaacctc attccttctt tggagcaggc acgttgggta
     1981 gaggtcctgg ggttctctga gtccagcagg aaggaatatt tctacagata tttcacagat
     2041 gaaaggcaag caattagagc ctttaggttg gtcaaatcaa acaaagagct ctgggccctg
     2101 tgtcttgtgc cctgggtgtc ctggctggcc tgcacttgcc tgatgcagca gatgaagcgg
     2161 aaggaaaaac tcacactgac ttccaagacc accacaaccc tctgtctaca ttaccttgcc
     2221 caggctctcc aagctcagcc attgggaccc cagctcagag acctctgctc tctggctgct
     2281 gagggcatct ggcaaaaaaa gacccttttc agtccagatg acctcaggaa gcatgggtta
     2341 gatggggcca tcatctccac cttcttgaag atgggtattc ttcaagagca ccccatccct
     2401 ctgagctaca gcttcattca cctctgtttc caagagttct ttgcagcaat gtcctatgtc
     2461 ttggaggatg agaaggggag aggtaaacat tctaattgca tcatagattt ggaaaagacg
     2521 ctagaagcat atggaataca tggcctgttt ggggcatcaa ccacacgttt cctattgggc
     2581 ctgttaagtg atgaggggga gagagagatg gagaacatct ttcactgccg gctgtctcag
     2641 gggaggaacc tgatgcagtg ggtcccgtcc ctgcagctgc tgctgcagcc acactctctg
     2701 gagtccctcc actgcttgta cgagactcgg aacaaaacgt tcctgacaca agtgatggcc
     2761 catttcgaag aaatgggcat gtgtgtagaa acagacatgg agctcttagt gtgcactttc
     2821 tgcattaaat tcagccgcca cgtgaagaag cttcagctga ttgagggcag gcagcacaga
     2881 tcaacatgga gccccaccat ggtagtcctg ttcaggtggg tcccagtcac agatgcctat
     2941 tggcagattc tcttctccgt cctcaaggtc accagaaacc tgaaggagct ggacctaagt
     3001 ggaaactcgc tgagccactc tgcagtgaag agtctttgta agaccctgag acgccctcgc
     3061 tgcctcctgg agaccctgcg gttggctggc tgtggcctca cagctgagga ctgcaaggac
     3121 cttgcctttg ggctgagagc caaccagacc ctgaccgagc tggacctgag cttcaatgtg
     3181 ctcacggatg ctggagccaa acacctttgc cagagactga gacagccgag ctgcaagcta
     3241 cagcgactgc agctggtcag ctgtggcctc acgtctgact gctgccagga cctggcctct
     3301 gtgcttagtg ccagccccag cctgaaggag ctagacctgc agcagaacaa cctggatgac
     3361 gttggcgtgc gactgctctg tgaggggctc aggcatcctg cctgcaaact catacgcctg
     3421 gggctggacc agacaactct gagtgatgag atgaggcagg aactgagggc cctggagcag
     3481 gagaaacctc agctgctcat cttcagcaga cggaaaccaa gtgtgatgac ccctactgag
     3541 ggcctggata cgggagagat gagtaatagc acatcctcac tcaagcggca gagactcgga
     3601 tcagagaggg cggcttccca tgttgctcag gctaatctca aactcctgga cgtgagcaag
     3661 atcttcccaa ttgctgagat tgcagaggaa agctccccag aggtagtacc ggtggaactc
     3721 ttgtgcgtgc cttctcctgc ctctcaaggg gacctgcata cgaagccttt ggggactgac
     3781 gatgacttct ggggccccac ggggcctgtg gctactgagg tagttgacaa agaaaagaac
     3841 ttgtaccgag ttcacttccc tgtagctggc tcctaccgct ggcccaacac gggtctctgc
     3901 tttgtgatga gagaagcggt gaccgttgag attgaattct gtgtgtggga ccagttcctg
     3961 ggtgagatca acccacagca cagctggatg gtggcagggc ctctgctgga catcaaggct
     4021 gagcctggag ctgtggaagc tgtgcacctc cctcactttg tggctctcca agggggccat
     4081 gtggacacat ccctgttcca aatggcccac tttaaagagg aggggatgct cctggagaag
     4141 ccagccaggg tggagctgca tcacatagtt ctggaaaacc ccagcttctc ccccttggga
     4201 gtcctcctga aaatgatcca taatgccctg cgcttcattc ccgtcacctc tgtggtgttg
     4261 ctttaccacc gcgtccatcc tgaggaagtc accttccacc tctacctgat cccaagtgac
     4321 tgctccattc ggaaggccat agatgatcta gaaatgaaat tccagtttgt gcgaatccac
     4381 aagccacccc cgctgacccc actttatatg ggctgtcgtt acactgtgtc tgggtctggt
     4441 tcagggatgc tggaaatact ccccaaggaa ctggagctct gctatcgaag ccctggagaa
     4501 gaccagctgt tctcggagtt ctacgttggc cacttgggat cagggatcag gctgcaagtg
     4561 aaagacaaga aagatgagac tctggtgtgg gaggccttgg tgaaaccagg agatctcatg
     4621 cctgcaacta ctctgatccc tccagcccgc atagccgtac cttcacctct ggatgccccg
     4681 cagttgctgc actttgtgga ccagtatcga gagcagctga tagcccgagt gacatcggtg
     4741 gaggttgtct tggacaaact gcatggacag gtgctgagcc aggagcagta cgagagggtg
     4801 ctggctgaga acacgaggcc cagccagatg cggaagctgt tcagcttgag ccagtcctgg
     4861 gaccggaagt gcaaagatgg actctaccaa gccctgaagg agacccatcc tcacctcatt
     4921 atggaactct gggagaaggg cagcaaaaag ggactcctgc cactcagcag ctgaagtatc
     4981 aacaccagcc cttgaccctt gagtcctggc tttggctgac ccttctttgg gtctcagttt
     5041 ctttctctgc aaacaagttg ccatctggtt tgccttccag cactaaagta atggaacttt
     5101 gatgatgcct ttgctgggca ttatgtgtcc atgccaggga tgccacaggg ggccccagtc
     5161 caggtggcct aacagcatct cagggaatgt ccatctggag ctggcaagac ccctgcagac
     5221 ctcatagagc ctcatctggt ggccacagca gccaagccta gagccctccg gatcccatcc
     5281 aggcgcaaag aggaatagga gggacatgga accatttgcc tctggctgtg tcacagggtg
     5341 agccccaaaa ttggggttca gcgtgggagg ccacgtggat tcttggcttt gtacaggaag
     5401 atctacaaga gcaagccaac agagtaaagt ggaaggaagt ttattcagaa aataaaggag
     5461 tatcacagct cttttagaat ttgtctagca ggctttccag tttttaccag aaaaccccta
     5521 taaattaaaa attttttact taaatttaag aattaaaaaa atacaaaaaa gaaaaaatga
     5581 aaataaagga ataagaagtt acctactcca
//
