LOCUS       NM_020726               8652 bp    mRNA    linear   PRI 24-SEP-2024
DEFINITION  Homo sapiens neurolysin (NLN), mRNA; nuclear gene for mitochondrial
            product.
ACCESSION   NM_020726
VERSION     NM_020726.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8652)
  AUTHORS   Shi,K., Bagchi,S., Bickel,J., Esfahani,S.H., Yin,L., Cheng,T.,
            Karamyan,V.T. and Aihara,H.
  TITLE     Structural basis of divergent substrate recognition and inhibition
            of human neurolysin
  JOURNAL   Sci Rep 14 (1), 18420 (2024)
   PUBMED   39117724
  REMARK    GeneRIF: Structural basis of divergent substrate recognition and
            inhibition of human neurolysin.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8652)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   3  (bases 1 to 8652)
  AUTHORS   Mirali,S., Botham,A., Voisin,V., Xu,C., St-Germain,J., Sharon,D.,
            Hoff,F.W., Qiu,Y., Hurren,R., Gronda,M., Jitkova,Y., Nachmias,B.,
            MacLean,N., Wang,X., Arruda,A., Minden,M.D., Horton,T.M.,
            Kornblau,S.M., Chan,S.M., Bader,G.D., Raught,B. and Schimmer,A.D.
  TITLE     The mitochondrial peptidase, neurolysin, regulates respiratory
            chain supercomplex formation and is necessary for AML viability
  JOURNAL   Sci Transl Med 12 (538) (2020)
   PUBMED   32269163
  REMARK    GeneRIF: The mitochondrial peptidase, neurolysin, regulates
            respiratory chain supercomplex formation and is necessary for AML
            viability.
REFERENCE   4  (bases 1 to 8652)
  AUTHORS   Teixeira,P.F., Masuyer,G., Pinho,C.M., Branca,R.M.M., Kmiec,B.,
            Wallin,C., Warmlander,S.K.T.S., Berntsson,R.P., Ankarcrona,M.,
            Graslund,A., Lehtio,J., Stenmark,P. and Glaser,E.
  TITLE     Mechanism of Peptide Binding and Cleavage by the Human
            Mitochondrial Peptidase Neurolysin
  JOURNAL   J Mol Biol 430 (3), 348-362 (2018)
   PUBMED   29183787
  REMARK    GeneRIF: The crystal structure of human neurolysin(E475Q) in
            complex with the products of neurotensin cleavage at 2.7A revealed
            a closed conformation with an internal cavity that restricts
            substrate length and highlighted the mechanism of enzyme
            opening/closing that is necessary for substrate binding and
            catalytic activity.
REFERENCE   5  (bases 1 to 8652)
  AUTHORS   Norman,M.U., Reeve,S.B., Dive,V., Smith,A.I. and Lew,R.A.
  TITLE     Endopeptidases 3.4.24.15 and 24.16 in endothelial cells: potential
            role in vasoactive peptide metabolism
  JOURNAL   Am J Physiol Heart Circ Physiol 284 (6), H1978-H1984 (2003)
   PUBMED   12609826
REFERENCE   6  (bases 1 to 8652)
  AUTHORS   Garrido,P.A., Vandenbulcke,F., Ramjaun,A.R., Vincent,B.,
            Checler,F., Ferro,E. and Beaudet,A.
  TITLE     Confocal microscopy reveals thimet oligopeptidase (EC 3.4.24.15)
            and neurolysin (EC 3.4.24.16) in the classical secretory pathway
  JOURNAL   DNA Cell Biol 18 (4), 323-331 (1999)
   PUBMED   10235115
REFERENCE   7  (bases 1 to 8652)
  AUTHORS   Rioli,V., Kato,A., Portaro,F.C., Cury,G.K., te Kaat,K., Vincent,B.,
            Checler,F., Camargo,A.C., Glucksman,M.J., Roberts,J.L., Hirose,S.
            and Ferro,E.S.
  TITLE     Neuropeptide specificity and inhibition of recombinant isoforms of
            the endopeptidase 3.4.24.16 family: comparison with the related
            recombinant endopeptidase 3.4.24.15
  JOURNAL   Biochem Biophys Res Commun 250 (1), 5-11 (1998)
   PUBMED   9735321
REFERENCE   8  (bases 1 to 8652)
  AUTHORS   Krause,D.R., Piva,T.J., Brown,S.B. and Ellem,K.A.
  TITLE     Characterization and localization of mitochondrial oligopeptidase
            (MOP) (EC 3.4.24.16) activity in the human cervical adenocarcinoma
            cell line HeLa
  JOURNAL   J Cell Biochem 66 (3), 297-308 (1997)
   PUBMED   9257187
REFERENCE   9  (bases 1 to 8652)
  AUTHORS   Vincent,B., Dauch,P., Vincent,J.P. and Checler,F.
  TITLE     Stably transfected human cells overexpressing rat brain
            endopeptidase 3.4.24.16: biochemical characterization of the
            activity and expression of soluble and membrane-associated
            counterparts
  JOURNAL   J Neurochem 68 (2), 837-845 (1997)
   PUBMED   9003076
REFERENCE   10 (bases 1 to 8652)
  AUTHORS   Vincent,B., Vincent,J.P. and Checler,F.
  TITLE     Purification and characterization of human endopeptidase 3.4.24.16.
            Comparison with the porcine counterpart indicates a unique cleavage
            site on neurotensin
  JOURNAL   Brain Res 709 (1), 51-58 (1996)
   PUBMED   8869556
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from CB992921.1, AJ300837.1,
            AC016643.6 and CA441264.1.
            
            On Nov 23, 2018 this sequence version replaced NM_020726.4.
            
            Summary: This gene encodes a member of the metallopeptidase M3
            protein family that cleaves neurotensin at the Pro10-Tyr11 bond,
            leading to the formation of neurotensin(1-10) and
            neurotensin(11-13). The encoded protein is likely involved in the
            termination of the neurotensinergic signal in the central nervous
            system and in the gastrointestinal tract.[provided by RefSeq, Jun
            2010].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB033052.2, SRR1803615.18418.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000380985.10/
                                                  ENSP00000370372.5
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-95                CB992921.1         37-131
            96-2934             AJ300837.1         52-2890
            2935-8119           AC016643.6         96001-101185
            8120-8652           CA441264.1         12-544              c
FEATURES             Location/Qualifiers
     source          1..8652
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5q12.3"
     gene            1..8652
                     /gene="NLN"
                     /gene_synonym="AGTBP; EP24.16; MEP; MOP"
                     /note="neurolysin"
                     /db_xref="GeneID:57486"
                     /db_xref="HGNC:HGNC:16058"
                     /db_xref="MIM:611530"
     exon            1..210
                     /gene="NLN"
                     /gene_synonym="AGTBP; EP24.16; MEP; MOP"
                     /inference="alignment:Splign:2.1.0"
     CDS             170..2284
                     /gene="NLN"
                     /gene_synonym="AGTBP; EP24.16; MEP; MOP"
                     /EC_number="3.4.24.16"
                     /note="endopeptidase 24.16; neurotensin endopeptidase;
                     angiotensin binding protein; microsomal endopeptidase;
                     mitochondrial oligopeptidase M; neurolysin
                     (metallopeptidase M3 family)"
                     /codon_start=1
                     /product="neurolysin, mitochondrial"
                     /protein_id="NP_065777.1"
                     /db_xref="CCDS:CCDS3989.1"
                     /db_xref="GeneID:57486"
                     /db_xref="HGNC:HGNC:16058"
                     /db_xref="MIM:611530"
                     /translation="MIARCLLAVRSLRRVGGSRILLRMTLGREVMSPLQAMSSYTVAG
                     RNVLRWDLSPEQIKTRTEELIVQTKQVYDAVGMLGIEEVTYENCLQALADVEVKYIVE
                     RTMLDFPQHVSSDKEVRAASTEADKRLSRFDIEMSMRGDIFERIVHLQETCDLGKIKP
                     EARRYLEKSIKMGKRNGLHLPEQVQNEIKSMKKRMSELCIDFNKNLNEDDTFLVFSKA
                     ELGALPDDFIDSLEKTDDDKYKITLKYPHYFPVMKKCCIPETRRRMEMAFNTRCKEEN
                     TIILQQLLPLRTKVAKLLGYSTHADFVLEMNTAKSTSRVTAFLDDLSQKLKPLGEAER
                     EFILNLKKKECKDRGFEYDGKINAWDLYYYMTQTEELKYSIDQEFLKEYFPIEVVTEG
                     LLNTYQELLGLSFEQMTDAHVWNKSVTLYTVKDKATGEVLGQFYLDLYPREGKYNHAA
                     CFGLQPGCLLPDGSRMMAVAALVVNFSQPVAGRPSLLRHDEVRTYFHEFGHVMHQICA
                     QTDFARFSGTNVETDFVEVPSQMLENWVWDVDSLRRLSKHYKDGSPIADDLLEKLVAS
                     RLVNTGLLTLRQIVLSKVDQSLHTNTSLDAASEYAKYCSEILGVAATPGTNMPATFGH
                     LAGGYDGQYYGYLWSEVFSMDMFYSCFKKEGIMNPEVGMKYRNLILKPGGSLDGMDML
                     HNFLKREPNQKAFLMSRGLHAP"
     exon            211..470
                     /gene="NLN"
                     /gene_synonym="AGTBP; EP24.16; MEP; MOP"
                     /inference="alignment:Splign:2.1.0"
     exon            471..619
                     /gene="NLN"
                     /gene_synonym="AGTBP; EP24.16; MEP; MOP"
                     /inference="alignment:Splign:2.1.0"
     exon            620..727
                     /gene="NLN"
                     /gene_synonym="AGTBP; EP24.16; MEP; MOP"
                     /inference="alignment:Splign:2.1.0"
     exon            728..830
                     /gene="NLN"
                     /gene_synonym="AGTBP; EP24.16; MEP; MOP"
                     /inference="alignment:Splign:2.1.0"
     exon            831..991
                     /gene="NLN"
                     /gene_synonym="AGTBP; EP24.16; MEP; MOP"
                     /inference="alignment:Splign:2.1.0"
     exon            992..1127
                     /gene="NLN"
                     /gene_synonym="AGTBP; EP24.16; MEP; MOP"
                     /inference="alignment:Splign:2.1.0"
     exon            1128..1494
                     /gene="NLN"
                     /gene_synonym="AGTBP; EP24.16; MEP; MOP"
                     /inference="alignment:Splign:2.1.0"
     exon            1495..1696
                     /gene="NLN"
                     /gene_synonym="AGTBP; EP24.16; MEP; MOP"
                     /inference="alignment:Splign:2.1.0"
     exon            1697..1883
                     /gene="NLN"
                     /gene_synonym="AGTBP; EP24.16; MEP; MOP"
                     /inference="alignment:Splign:2.1.0"
     exon            1884..2012
                     /gene="NLN"
                     /gene_synonym="AGTBP; EP24.16; MEP; MOP"
                     /inference="alignment:Splign:2.1.0"
     exon            2013..2149
                     /gene="NLN"
                     /gene_synonym="AGTBP; EP24.16; MEP; MOP"
                     /inference="alignment:Splign:2.1.0"
     exon            2150..8652
                     /gene="NLN"
                     /gene_synonym="AGTBP; EP24.16; MEP; MOP"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2685..2690
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NLN"
                     /gene_synonym="AGTBP; EP24.16; MEP; MOP"
                     /note="hexamer: AATGAA"
     polyA_site      2723
                     /gene="NLN"
                     /gene_synonym="AGTBP; EP24.16; MEP; MOP"
     regulatory      4029..4034
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NLN"
                     /gene_synonym="AGTBP; EP24.16; MEP; MOP"
                     /note="hexamer: AATAAA"
     polyA_site      4056
                     /gene="NLN"
                     /gene_synonym="AGTBP; EP24.16; MEP; MOP"
                     /note="major polyA site"
     regulatory      8632..8637
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NLN"
                     /gene_synonym="AGTBP; EP24.16; MEP; MOP"
                     /note="hexamer: AATATA"
     polyA_site      8652
                     /gene="NLN"
                     /gene_synonym="AGTBP; EP24.16; MEP; MOP"
ORIGIN      
        1 aggcgccggc gtggagctgc cgcacgtggg agggcgctgg ccaggcagcc actgtggcct
       61 ctgcggctag gccggctcga gactcccggg cgcccaggcg ctgccgcccg cctcgccgcc
      121 ccacgccgaa ggaccacgcg cccgccgccg ccagcctctc agcgctccca tgatcgcccg
      181 gtgccttttg gctgtgcgaa gcctccgcag agttggtggt tccaggattt tactcagaat
      241 gacgttagga agagaagtga tgtctcctct tcaggcaatg tcttcctata ctgtggctgg
      301 cagaaatgtt ttaagatggg atctttcacc agagcaaatt aaaacaagaa ctgaggagct
      361 cattgtgcag accaaacagg tgtacgatgc tgttggaatg ctcggtattg aggaagtaac
      421 ttacgagaac tgtctgcagg cactggcaga tgtagaagta aagtatatag tggaaaggac
      481 catgctagac tttccccagc atgtatcctc tgacaaagaa gtacgagcag caagtacaga
      541 agcagacaaa agactttctc gttttgatat tgagatgagc atgagaggag atatatttga
      601 gagaattgtt catttacagg aaacctgtga tctggggaag ataaaacctg aggccagacg
      661 atacttggaa aagtcaatta aaatggggaa aagaaatggg ctccatcttc ctgaacaagt
      721 acagaatgaa atcaaatcaa tgaagaaaag aatgagtgag ctatgtattg attttaacaa
      781 aaacctcaat gaggatgata ccttccttgt attttccaag gctgaacttg gtgctcttcc
      841 tgatgatttc attgacagtt tagaaaagac agatgatgac aagtataaaa ttaccttaaa
      901 atatccacac tatttccctg tcatgaagaa atgttgtatc cctgaaacca gaagaaggat
      961 ggaaatggct tttaatacaa ggtgcaaaga ggaaaacacc ataattttgc agcagctact
     1021 cccactgcga accaaggtgg ccaaactact cggttatagc acacatgctg acttcgtcct
     1081 tgaaatgaac actgcaaaga gcacaagccg cgtaacagcc tttctagatg atttaagcca
     1141 gaagttaaaa cccttgggtg aagcagaacg agagtttatt ttgaatttga agaaaaagga
     1201 atgcaaagac aggggttttg aatatgatgg gaaaatcaat gcctgggatc tatattacta
     1261 catgactcag acagaggaac tcaagtattc catagaccaa gagttcctca aggaatactt
     1321 cccaattgag gtggtcactg aaggcttgct gaacacctac caggagttgt tgggactttc
     1381 atttgaacaa atgacagatg ctcatgtttg gaacaagagt gttacacttt atactgtgaa
     1441 ggataaagct acaggagaag tattgggaca gttctatttg gacctctatc caagggaagg
     1501 aaaatacaat catgcggcct gcttcggtct ccagcctggc tgccttctgc ctgatggaag
     1561 ccggatgatg gcagtggctg ccctcgtggt gaacttctca cagccagtgg caggtcgtcc
     1621 ctctctcctg agacacgacg aggtgaggac ttactttcat gagtttggtc acgtgatgca
     1681 tcagatttgt gcacagactg attttgcacg atttagcgga acaaatgtgg aaactgactt
     1741 tgtagaggtg ccatcgcaaa tgcttgaaaa ttgggtgtgg gacgtcgatt ccctccgaag
     1801 attgtcaaaa cattataaag atggaagccc tattgcagac gatctgcttg aaaaacttgt
     1861 tgcttctagg ctggtcaaca caggtcttct gaccctgcgc cagattgttt tgagcaaagt
     1921 tgatcagtct cttcatacca acacatcgct ggatgctgca agtgaatatg ccaaatactg
     1981 ctcagaaata ttaggagttg cagctactcc aggcacaaat atgccagcta cctttggaca
     2041 tttggcaggg ggatacgatg gccaatatta tggatatctt tggagtgaag tattttccat
     2101 ggatatgttt tacagctgtt ttaaaaaaga agggataatg aatccagagg ttggaatgaa
     2161 atacagaaac ctaatcctga aacctggggg atctctggac ggcatggaca tgctccacaa
     2221 tttcttgaaa cgtgagccaa accaaaaagc gttcctaatg agtagaggcc tgcatgctcc
     2281 gtgaactggg gatctttggt agccgtccat gtctggagga caagtcgaca tcaccatgtg
     2341 ttactggcct ggaaactgaa gggagttttg caagtgaaaa tttagatttc tattgacatc
     2401 cttttgtttt ctaattttaa aaattataaa gatgtaaatg gaattataaa tactgtgacc
     2461 taagaaaaga cccactagaa agtaattgta ctataaaatt tcataaaact ggatttgatt
     2521 tctttttatg aaagtttcat atgaatgtaa cttgattttt tactattata atctagataa
     2581 tatgatataa gagggctaag aatttttaaa ttgaatcata tatatgatat aatttgatcc
     2641 ttcttgtatc ttgaagtttt gtacttggga tttctggact gataaatgaa tcatcacatt
     2701 cttctggtaa atattttctt ggagctctgt gtcaactttg atcctttgtc tcccaggaag
     2761 gtgtgacctc tcctttgcct gcatacctca aggccagggg aatatgcctc agtgatgcat
     2821 ttatctttgt atatcaggcc gcatgattcc caactttctg ccacacttaa attacgttcc
     2881 tccatttcag ttttgtcttt tctgtctaaa gttcagtcaa agagtatcaa aaaattatgt
     2941 ttcagctaga ctggtgtaat gtataagttt ttgtatcttg tattagagga tttcgtagct
     3001 tttattagag gctcatttcc acctcagcat acaagatcgt tagtcttttg gcatgtgtgc
     3061 caattagaat actaaagcaa gtccaagcac atttttctct tctcacgttt ctaataagtg
     3121 ttagggactt tgcctctttt acttaccacg tccccaaaag tgtcaggtag acatgtcaca
     3181 aatggctctg tagagagcca tgggaagaga gaggaggtgg atgtggaaca taaagggttc
     3241 agaaactcca gaagaggagt gggttttgga tagaagcatt tgaggacagc tgctccaaag
     3301 ccttatgtgt atgatgaaac ttaaccacgg ggaagagact cttcagtagc ctgttctgtc
     3361 tggtgatttt tattttaagt gaacctttgg atctatcttt aactctcttt attgtgagtc
     3421 taaattccaa ttctgcagca gatcagtaaa ctcacagtat ttttcctgtg gaaatctatt
     3481 caataaggaa accaagacag gataataaaa tttaaaaaaa aaacaacttt gaattcccct
     3541 gcctaggtct tccagttgtt ttccagcgca tacctcaggt atgactttgc tagccgggga
     3601 caaaattagc accttccgat tctctagtcc aaatgaactt tgtgctaaat aaaaaattat
     3661 tatactacat aataaagtta cagatagcag gaaatgcaag agctaggaga ttcctagatt
     3721 atatctgcca agcaaatacc ttaaacatcc acctgaaatc ctactacccc ctcttctgag
     3781 ataatttgcc cagcccttct cttcccacac actcactcaa tgtcaccccc ttctaatccc
     3841 caaaactgtt tttgtggtct ttgtagccta tagtagtttt ctcacatctt tccccctaga
     3901 cttttctgtt tttcagtttc agacaaaaaa actcttcagc tttttccagt gtgtctcctt
     3961 aacagtaact ttaccacttg aaatcttatt tcatagaaaa actaaattgg tgtggaaagg
     4021 ctgcacacaa taaagttata ttattatcca tgaaaatgaa ctcatatttc tttcatactt
     4081 taacgttaaa accgaaatgc atgagagcaa aagcaccatg gtgttctttc tatttagggc
     4141 ctacctctaa tatttaaaat ctaccaaaga gcagtcacaa aattaaaact cagcccgggc
     4201 gcagtggctc atgcctgtaa tcccagcact ttgggaggcc aaggcaggag gatcacctga
     4261 ggtcagagtt caagaccagc atggtcaaca tggtgaaacc ctgtctctac aaaaatacaa
     4321 aaagctgggc atgatgttgg gttcctgtag tcccagctac ttaggaggct gaggcaggag
     4381 aatagcttga acccgggagg tggaggttgc agtgagctga gatcatgcca ttgcactcca
     4441 gcctggacga cagagtgaga ttcagtctca aaaaaaatta atactcaaag aattatctag
     4501 cataatttaa aagaaaggac tttttaaaaa ttctctagca taattcaaaa gaaaggactt
     4561 aacttttttt tttttagtgt ggtcatccgt ctagttgttc tttttttcca gatcagagca
     4621 gagaatatca ctgacatgct tgaaatggtg gataactatg taaattgtaa ttggacaaat
     4681 gtacacttta gatttatgga ctgagccaca tataataagg tctctcctgg taattgatcc
     4741 aggggattta ggcctctttc gggttttttg tttttttttt aggcattgtt atgttgtgaa
     4801 ggataaaatc ttcaacatct tatgcaaatg gatagtaggc atgatcctaa aggtttagtt
     4861 ttacgatgct gcagagaaga gaaatgtctt gacgttttgc cacctgatgt agactttgtc
     4921 cccctctagt ataaatgttg catgttacct agataaacaa ctaaaaattg ccttgagtta
     4981 tcacctgagc tacttatgcc aaggtcttgc ctttctaaag ctaataaggt gagaggtgga
     5041 tatctgtagc tcttcggatg aaaaattgca ttgtgggaga taccaaaatt gaggaaatag
     5101 ctcttcaaag aaaaattact gatatgatca ttgtacttgt aaatgcctta agtagcaggc
     5161 agtgactcaa ttttctactt taccatttta cctttagctt ttatgtatga attataggtc
     5221 tgtggagatt ctgcctcccc actaggccag tgtgtgttta ccatttattc attttctatc
     5281 atacacaggt ggattaaaat ttacattaaa atttacatta aaataccatc atacttcagg
     5341 cttctataac aaaatatcag actgggtggc ttcaaaagca gacatttctt acagttctgg
     5401 aagctgggaa gtcccacaat ccacatgctg gtaaatttgg ttcgtggtga ggcccctctt
     5461 cctcttttac tgaccacatc cccaaaagtg tcaggtagac atgttacaaa tagctctgta
     5521 gagagccatg ggaagagaca ggaggcagat gtggaacata aagggtttag aaacttctgc
     5581 aggcagcagt ggcagggagc aagccctaga gtgtctcttt ttacgagagc aggaatccca
     5641 tcatgagagt tccttcctca tgactttatc taaacccagt cacccccaac aaaggcccca
     5701 ccccctaacc ccatcacatt ggctgttacg gcttcaatgt aggggatggg gcacaaacat
     5761 tcagtccata acgaatactg attcctcaaa tagggtttaa acatagttaa ctgagccccc
     5821 aaagaatgtt aaaagagaaa atccatttta tttgaataga tgcagtgacc acagcttctt
     5881 ccctcaaatc aacacattac agttagatgt ctcccatctg aaattggata gcccactgaa
     5941 attgaacatg ccttctctta taaatgtgtg gtgagaataa aagcaacaaa gaaatgaatg
     6001 tggtggctca cacctgtaat cctaacactt tggaaggttg aggcaggagg atcctttgtg
     6061 ctcaagagtt tgagaccagc ctgagcaaca cagtgagacc ctgtctctac aaaaactaaa
     6121 aaaattagct agggatggtg gcacatgcct gtagtcccag ctacttggga ggctgaggtg
     6181 ggaggattgc ttgagcccaa gaaggtcaag gctgccatga gccataatca cgccactgca
     6241 ctccagcctg ggtgacagag accctgtctt aaaataaaat aaaaaaaaaa ggaaaaggaa
     6301 agacatacat accctcagtt cttcgaaaaa cagtcaacgt ccaaaaagtt tattttttga
     6361 gtcaccttta atagagaaat ttcccactgg aaacagatcc acctaagtga ttatctagta
     6421 tcagctgtat caaggcaaaa ggtaaaggtt ataagatgta aatattagac caaaatagcc
     6481 ttttttaaca aacacaataa tgctgtacgt ctgtaacaaa acaaaatttg aaaaattgag
     6541 gtaatgtaca cagcgatgca cacaccccca caccacaccg tgattcctga gttttgattt
     6601 ggttgaattc accttgtagt tccctttcct gagaaaatac aggttcaaca catctagtcc
     6661 acgatgtacg tacaagggga aggggtgaac aggaaataga ctcccttatt ctgctcacat
     6721 gagctggaac ctgtactagc tgcatatttc atagacccca gaggctcacc tcaattgtac
     6781 tcctatgata aaatgtctct agcatttgtc atgagagaaa ctggctagaa atagtttgtt
     6841 cattgcgtcc agcaagacac gcattgctgt tgatatcttt gtggctaaga tggagaatgt
     6901 gactggataa tagttatcca ggtggattac tagctattga aacagtctga gatagttgca
     6961 tgatgattaa cgttaaactg ggagtaattt caagtcattt ggctaaaaat gaaaaaaaag
     7021 tcccataaaa aggtatcaaa tgtacagata atagggctgg aattattaag tatgatgatg
     7081 ttattaacat ctaaaattat tttggccggg caaggtgggt cacgcctgta atctcagcac
     7141 tttgggatgc caagatgggt ggatcacctg agttcaggag ttcaagacca gcttggccaa
     7201 catggcgaaa cccgtctcta ctaaagaata caaaaattag ccaggtgttg tggcacatgc
     7261 ctgtactccc agctactcag gaggctgagg cagaagaatc atttgaaccc cggaggtgga
     7321 ggttgcagtg agccgacatt gtgccgcttc attccagcct gggcaacaga gtgaaactct
     7381 tgtctcaaaa gtaaaaaaat aataatgttt aaaaatattt caatgtggag acaagctcaa
     7441 aatgaaatta gacacattcc attacccagg taaaagaagg ggaagcctga cttgatagta
     7501 gtattcagga aaaaagagtt ggcagtttta tttggccaaa ttccaatatc agctcatggt
     7561 acagcacacc gggggagggg gacgggaggc gagaactaag gctttttaag aatgtgttga
     7621 tggaagtatg tgcctagatc aaaagaataa tccccccgga ctccagtgta agatcaatta
     7681 ctgttggaat attgtgttcc tttctagata tcacatttta agcagacttt ggccaagtag
     7741 tacagtgttt gcaggagcag taacaagatg gtgataactt tgaaaatact tctcaaaaga
     7801 aaaataaaaa agaattaggg aagttcagtc tggagataat tcagaaatac agatgataat
     7861 tgttttcaaa ttcttgaagg aaatgggaga gagaataggc taattctgta ttgcttcaga
     7921 aaccaaatgg aaacaattaa attccattag agaaacggtt ggaaaatatg aggaggattt
     7981 agttcagtac gaggaagctg tttctaatac tttttgttgg ttaaaaatac attccgctgc
     8041 tgtggaaagc ctgtagctgc caggagtgtt acagagggca ttcctccctt gagttgacat
     8101 ttgtgccaaa ccggctttta gggcttctcc agcttttaag atgcgataat gataagatga
     8161 tcatcgggaa aacatccctc agatgaaacg ttcacaggct ggctctgaaa actggcattc
     8221 agatgattgc ggctccccca tactgtagga atattgttta tggcttaagg ttgcctcgct
     8281 cctcatctgt aatcagtgaa agtttatcca gaggttaatt accggttttc tggtgggtat
     8341 ctgggaactg aggatgggag attaactgtt gtatatgacc atagtaaagc aaaagactgt
     8401 tagagaatga gtaaacgttc gttttcctcc ctacagaatt tacagttact ctctgtccat
     8461 tccactcact actgtcttca tcacacacac agaggcaaac gtctgattca gatagccagg
     8521 tgtgccatgc atgcactcca ccatattgtg atctcttcga ggcttgtttc tgtcccctgt
     8581 cctccacagt cctggattgt taatgaatag ttcatacatg tttgttaaat aaatatacct
     8641 tctttaaaat ta
//
