LOCUS       NM_001365925           16241 bp    mRNA    linear   PRI 09-JUN-2024
DEFINITION  Homo sapiens neuroligin 1 (NLGN1), transcript variant 3, mRNA.
ACCESSION   NM_001365925 XM_017005890
VERSION     NM_001365925.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 16241)
  AUTHORS   Lee,I.H., Walker,D.I., Lin,Y., Smith,M.R., Mandl,K.D., Jones,D.P.
            and Kong,S.W.
  TITLE     Association between Neuroligin-1 polymorphism and plasma glutamine
            levels in individuals with autism spectrum disorder
  JOURNAL   EBioMedicine 95, 104746 (2023)
   PUBMED   37544204
  REMARK    GeneRIF: Association between Neuroligin-1 polymorphism and plasma
            glutamine levels in individuals with autism spectrum disorder.
REFERENCE   2  (bases 1 to 16241)
  AUTHORS   Pergolizzi,M., Bizzozero,L., Maione,F., Maldi,E., Isella,C.,
            Macagno,M., Mariella,E., Bardelli,A., Medico,E., Marchio,C.,
            Serini,G., Di Nicolantonio,F., Bussolino,F. and Arese,M.
  TITLE     The neuronal protein Neuroligin 1 promotes colorectal cancer
            progression by modulating the APC/beta-catenin pathway
  JOURNAL   J Exp Clin Cancer Res 41 (1), 266 (2022)
   PUBMED   36056393
  REMARK    GeneRIF: The neuronal protein Neuroligin 1 promotes colorectal
            cancer progression by modulating the APC/beta-catenin pathway.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 16241)
  AUTHORS   Choi,G.E., Chae,C.W., Park,M.R., Yoon,J.H., Jung,Y.H., Lee,H.J. and
            Han,H.J.
  TITLE     Prenatal glucocorticoid exposure selectively impairs neuroligin
            1-dependent neurogenesis by suppressing astrocytic FGF2-neuronal
            FGFR1 axis
  JOURNAL   Cell Mol Life Sci 79 (6), 294 (2022)
   PUBMED   35562616
  REMARK    GeneRIF: Prenatal glucocorticoid exposure selectively impairs
            neuroligin 1-dependent neurogenesis by suppressing astrocytic
            FGF2-neuronal FGFR1 axis.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 16241)
  AUTHORS   Yu,Q., Wang,X., Yang,Y., Chi,P., Huang,J., Qiu,S., Zheng,X. and
            Chen,X.
  TITLE     Upregulated NLGN1 predicts poor survival in colorectal cancer
  JOURNAL   BMC Cancer 21 (1), 884 (2021)
   PUBMED   34340665
  REMARK    GeneRIF: Upregulated NLGN1 predicts poor survival in colorectal
            cancer.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 16241)
  AUTHORS   Camporesi,E., Lashley,T., Gobom,J., Lantero-Rodriguez,J.,
            Hansson,O., Zetterberg,H., Blennow,K. and Becker,B.
  TITLE     Neuroligin-1 in brain and CSF of neurodegenerative disorders:
            investigation for synaptic biomarkers
  JOURNAL   Acta Neuropathol Commun 9 (1), 19 (2021)
   PUBMED   33522967
  REMARK    GeneRIF: Neuroligin-1 in brain and CSF of neurodegenerative
            disorders: investigation for synaptic biomarkers.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 16241)
  AUTHORS   Cantallops,I. and Cline,H.T.
  TITLE     Synapse formation: if it looks like a duck and quacks like a duck
            ...
  JOURNAL   Curr Biol 10 (17), R620-R623 (2000)
   PUBMED   10996085
  REMARK    Review article
REFERENCE   7  (bases 1 to 16241)
  AUTHORS   Scheiffele,P., Fan,J., Choih,J., Fetter,R. and Serafini,T.
  TITLE     Neuroligin expressed in nonneuronal cells triggers presynaptic
            development in contacting axons
  JOURNAL   Cell 101 (6), 657-669 (2000)
   PUBMED   10892652
REFERENCE   8  (bases 1 to 16241)
  AUTHORS   Irie,M., Hata,Y., Takeuchi,M., Ichtchenko,K., Toyoda,A., Hirao,K.,
            Takai,Y., Rosahl,T.W. and Sudhof,T.C.
  TITLE     Binding of neuroligins to PSD-95
  JOURNAL   Science 277 (5331), 1511-1515 (1997)
   PUBMED   9278515
REFERENCE   9  (bases 1 to 16241)
  AUTHORS   Ichtchenko,K., Nguyen,T. and Sudhof,T.C.
  TITLE     Structures, alternative splicing, and neurexin binding of multiple
            neuroligins
  JOURNAL   J Biol Chem 271 (5), 2676-2682 (1996)
   PUBMED   8576240
REFERENCE   10 (bases 1 to 16241)
  AUTHORS   Ichtchenko,K., Hata,Y., Nguyen,T., Ullrich,B., Missler,M.,
            Moomaw,C. and Sudhof,T.C.
  TITLE     Neuroligin 1: a splice site-specific ligand for beta-neurexins
  JOURNAL   Cell 81 (3), 435-443 (1995)
   PUBMED   7736595
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC018456.18, AC092967.5,
            AC092923.8 and AC008120.17.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_001365925.1.
            
            Summary: This gene encodes a member of a family of neuronal cell
            surface proteins. Members of this family may act as splice
            site-specific ligands for beta-neurexins and may be involved in the
            formation and remodeling of central nervous system synapses.
            [provided by RefSeq, Jul 2008].
            
            Sequence Note: The RefSeq transcript and protein were derived from
            genomic sequence to make the sequence consistent with the reference
            genome assembly. The genomic coordinates used for the transcript
            record were based on alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803614.109098.1,
                                           SRR1803613.128928.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA2467150, SAMN03267759
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000695368.1/ ENSP00000511841.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-320               AC018456.18        90355-90674         c
            321-1133            AC092967.5         56540-57352
            1134-1193           AC092967.5         57795-57854
            1194-1346           AC092923.8         67966-68118         c
            1347-1559           AC008120.17        22015-22227         c
            1560-2349           AC008120.17        17892-18681         c
            2350-16241          AC008120.17        3170-17061          c
FEATURES             Location/Qualifiers
     source          1..16241
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3q26.31"
     gene            1..16241
                     /gene="NLGN1"
                     /gene_synonym="NL1; NLG1"
                     /note="neuroligin 1"
                     /db_xref="GeneID:22871"
                     /db_xref="HGNC:HGNC:14291"
                     /db_xref="MIM:600568"
     exon            1..320
                     /gene="NLGN1"
                     /gene_synonym="NL1; NLG1"
                     /inference="alignment:Splign:2.1.0"
     exon            321..1133
                     /gene="NLGN1"
                     /gene_synonym="NL1; NLG1"
                     /inference="alignment:Splign:2.1.0"
     CDS             641..3172
                     /gene="NLGN1"
                     /gene_synonym="NL1; NLG1"
                     /note="isoform 2 precursor is encoded by transcript
                     variant 3"
                     /codon_start=1
                     /product="neuroligin-1 isoform 2 precursor"
                     /protein_id="NP_001352854.1"
                     /db_xref="CCDS:CCDS93427.1"
                     /db_xref="GeneID:22871"
                     /db_xref="HGNC:HGNC:14291"
                     /db_xref="MIM:600568"
                     /translation="MALPRCTWPNYVWRAVMACLVHRGLGAPLTLCMLGCLLQAGHVL
                     SQKLDDVDPLVATNFGKIRGIKKELNNEILGPVIQFLGVPYAAPPTGERRFQPPEPPS
                     PWSDIRNATQFAPVCPQNIIDGRLPEVMLPVWFTNNLDVVSSYVQDQSEDCLYLNIYV
                     PTEDVKRISKECARKPGKKICRKGDIRDSGGPKPVMVYIHGGSYMEGTGNLYDGSVLA
                     SYGNVIVITVNYRLGVLGFLSTGDQAAKGNYGLLDLIQALRWTSENIGFFGGDPLRIT
                     VFGSGAGGSCVNLLTLSHYSEGNRWSNSTKGLFQRAIAQSGTALSSWAVSFQPAKYAR
                     MLATKVGCNVSDTVELVECLQKKPYKELVDQDIQPARYHIAFGPVIDGDVIPDDPQIL
                     MEQGEFLNYDIMLGVNQGEGLKFVENIVDSDDGISASDFDFAVSNFVDNLYGYPEGKD
                     VLRETIKFMYTDWADRHNPETRRKTLLALFTDHQWVAPAVATADLHSNFGSPTYFYAF
                     YHHCQTDQVPAWADAAHGDEVPYVLGIPMIGPTELFPCNFSKNDVMLSAVVMTYWTNF
                     AKTGDPNQPVPQDTKFIHTKPNRFEEVAWTRYSQKDQLYLHIGLKPRVKEHYRANKVN
                     LWLELVPHLHNLNDISQYTSTTTKVPSTDITFRPTRKNSVPVTSAFPTAKQDDPKQQP
                     SPFSVDQRDYSTELSVTIAVGASLLFLNILAFAALYYKKDKRRHDVHRRCSPQRTTTN
                     DLTHAQEEEIMSLQMKHTDLDHECESIHPHEVVLRTACPPDYTLAMRRSPDDVPLMTP
                     NTITMIPNTIPGIQPLHTFNTFTGGQNNTLPHPHPHPHSHSTTRV"
     exon            1134..1193
                     /gene="NLGN1"
                     /gene_synonym="NL1; NLG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1194..1346
                     /gene="NLGN1"
                     /gene_synonym="NL1; NLG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1347..1559
                     /gene="NLGN1"
                     /gene_synonym="NL1; NLG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1560..2349
                     /gene="NLGN1"
                     /gene_synonym="NL1; NLG1"
                     /inference="alignment:Splign:2.1.0"
     exon            2350..16241
                     /gene="NLGN1"
                     /gene_synonym="NL1; NLG1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4947..4952
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NLGN1"
                     /gene_synonym="NL1; NLG1"
                     /note="hexamer: ATTAAA"
     polyA_site      4965
                     /gene="NLGN1"
                     /gene_synonym="NL1; NLG1"
                     /note="major polyA site"
     regulatory      7258..7263
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NLGN1"
                     /gene_synonym="NL1; NLG1"
                     /note="hexamer: AATAAA"
     polyA_site      7278
                     /gene="NLGN1"
                     /gene_synonym="NL1; NLG1"
     regulatory      8490..8495
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NLGN1"
                     /gene_synonym="NL1; NLG1"
                     /note="hexamer: AATAAA"
     polyA_site      8513
                     /gene="NLGN1"
                     /gene_synonym="NL1; NLG1"
     regulatory      16219..16224
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NLGN1"
                     /gene_synonym="NL1; NLG1"
                     /note="hexamer: AATAAA"
     polyA_site      16241
                     /gene="NLGN1"
                     /gene_synonym="NL1; NLG1"
ORIGIN      
        1 gccgcggctg taagccgagg atcaggcggc ggcgcggaga gcgcgcccgg gcggccagcg
       61 ccgagcgcgc ggagctggcg tggggagaca cggcaggaaa ggcgagtcgg agtgggtgat
      121 gagaggccgg gagagcccgt ccgagccgga gagaccggag agaagggagc gagcgactgt
      181 gcggcgctca cgctgccaga gcccgggcgg agtgcagtcg cctccaaagc cttagagcgg
      241 cgccccagtc actgcagctc ttcaccctag agctgtgccc tgggaccaca gagaccaaga
      301 gcatggaaat cggctgagag gatatagacc tgcagctaac tggatttgat ttataagaga
      361 gaaatctgca gtcaatgccc actcttgcca cactgctaat atggaaaaca gaatgttcaa
      421 taggatatgg tctgataaat agtgatgatt gaagatgctg ctccaataca tgtgaaatca
      481 atgggagata tctgctgtct gaagatcttt cagagctttt ctcgacaagc tcccctgtaa
      541 gaaatcggag gtatattcta ccattataca gtctttctca agtggatata aatacgtttg
      601 cctcactgta accagacaac tagacaacta atgtgggacc atggcactgc ccagatgcac
      661 gtggccaaat tatgtttgga gagcagtgat ggcatgcttg gtacaccggg gattgggtgc
      721 cccattgact ctctgtatgt tgggatgttt gcttcaggct ggccatgtgc tatcacaaaa
      781 attggatgat gtggacccac tggtggctac caactttgga aagataagag ggattaagaa
      841 ggaactcaat aatgaaattt tggggcctgt tattcaattt cttggggttc catatgcagc
      901 cccaccaaca ggggaacgtc gttttcagcc tccagaacca ccatctccct ggtcagatat
      961 cagaaatgcc actcaatttg ctcctgtgtg tccccagaat atcattgatg gcagattgcc
     1021 agaagtcatg cttcctgtgt ggtttactaa taacttggat gtggtttcat catatgtgca
     1081 agaccagagc gaagactgcc tatatttaaa tatatatgtc ccgactgagg atgtaaaaag
     1141 aatatccaag gaatgtgcca gaaagcccgg caagaaaata tgtagaaaag gagatattcg
     1201 ggacagtggg ggtcccaaac cagtgatggt gtatatccat ggtggctcat atatggaagg
     1261 tactggaaat ttatatgatg gaagtgtctt ggcaagttat ggcaatgtga tcgtcatcac
     1321 agtcaactat cgacttggag tactcggttt cttgagtaca ggcgatcagg ctgcaaaggg
     1381 gaactatgga ctccttgatc tcatacaagc tttaagatgg actagtgaaa acattggatt
     1441 ctttggtggt gaccccttaa gaatcactgt ttttggatct ggtgctgggg gttcatgtgt
     1501 caacctgctg actttatccc attattctga aggtaaccgt tggagcaatt caaccaaagg
     1561 actttttcaa cgagcaatag ctcaaagtgg aacagccctt tccagctggg ctgttagttt
     1621 tcaacctgca aaatatgcta gaatgttggc cacaaaagtt ggttgcaatg tttcagatac
     1681 agtagagtta gtggaatgcc tacagaagaa gccttacaaa gaacttgttg accaagatat
     1741 tcaaccagct cgataccaca tagcctttgg acctgtgatt gatggtgatg taataccaga
     1801 cgacccccag atattgatgg agcaaggaga gtttctcaac tatgatataa tgttaggagt
     1861 gaaccaaggg gaagggttaa aatttgttga aaatatagta gatagcgatg atggtatatc
     1921 agctagtgat tttgactttg ctgtttcaaa ttttgttgat aatttatatg gatatcctga
     1981 aggcaaagat gttttgagag aaaccattaa gttcatgtat actgactggg ctgaccgtca
     2041 taaccctgaa accagaagaa agacattact ggctttgttt acggaccatc agtgggtggc
     2101 accagctgta gccacagcgg atcttcactc aaactttggt tcacctacgt acttctatgc
     2161 cttttaccat cattgccaaa cagatcaggt tccagcttgg gctgatgcag cccacggaga
     2221 cgaggttccc tatgtactgg gaatccccat gattggccct acagagttat ttccttgcaa
     2281 tttctccaaa aatgatgtga tgctgagtgc agttgtaatg acatactgga caaattttgc
     2341 taaaactggt gacccaaatc aaccagtccc tcaagacacg aaattcattc ataccaaacc
     2401 caaccgtttt gaagaagtag catggaccag atattcccag aaagaccaac tttatctcca
     2461 tattggatta aaaccaagag ttaaagaaca ttacagagcc aataaggtga acctctggtt
     2521 ggagttggta cctcatctgc ataatctcaa tgacatttct cagtatacct ctacaacaac
     2581 taaagtgcca tcaactgaca tcactttcag acctacgaga aaaaattctg tacctgtcac
     2641 gtcagccttt cccactgcca agcaggatga tcccaaacaa caaccaagtc cattttcagt
     2701 ggatcaaagg gactactcaa cagagctgag tgtcactatt gcagttggag catcactgct
     2761 gtttctgaac atcttggcct ttgcagccct gtactacaaa aaggataaga ggagacatga
     2821 tgttcacagg agatgcagcc ctcagcgcac tactaccaat gatctaaccc atgcacaaga
     2881 agaggaaatc atgtccctcc aaatgaagca cactgatttg gatcatgaat gtgagtccat
     2941 tcatccacat gaggtggttc ttcggaccgc ctgtccccca gattacacac tagctatgag
     3001 gaggtcacct gatgatgttc ccttaatgac acccaacacc attacaatga ttcccaacac
     3061 tataccaggg attcagccct tacacacatt caatacattt actggaggac agaacaatac
     3121 tctgccccat ccccatcccc acccccattc acattcaaca accagggtat agccagataa
     3181 gagaaacaaa ctattttttt tgatggattg cagtaaacga tcactgaaga ttccttggct
     3241 ttcaacctac aagacttact atttaaataa ggaggaatat tatgtgaata tacatatcaa
     3301 gaactttggg ggttttgaaa aaaatgaatt gtatatatac aaatcaactt taaaaacaaa
     3361 tttcaattgc ttgaagcaat tgttctgaat gatacttttt cattcacatt caagaattaa
     3421 ttttttgaag atttaagtta cataatggaa ttaggcatgt ggaacaccaa acaggaaaga
     3481 actatgtctg aaatataaaa aataaaaata aaaaaacaac tatgaatatg cacaagggac
     3541 acaccagtgg aatgtcagat aattttcacc agtttttatt tggagccgtt ttattgtgta
     3601 gaccatattt acatatttgg ataagtacac aaagcgtcaa tgctgttaat ggccttagca
     3661 aaggctcatg ctgaaatttg ccagtaaaac aaagaagttt aaagactggc aggtacacca
     3721 ttatcacata agtgctgtca gtataaagtt gtggggataa aggaaactgg atatttttag
     3781 cacgatgtgc atgataattt atatgcttgg tggctgtgct gctgattaag ccgtaattaa
     3841 aattcttctc atcccattgg agtttttaat agaagcttcc tccatcaatt ggcagaacct
     3901 aaagaagatt ttaaggggca aaagtaatta caataaaata attcacagta gtttcaatat
     3961 agaaggaatt agctattaaa ggtatttgaa gaaactatag gtatagtggt gaatactcgc
     4021 tgatatgaat cccagaaaaa aatttcctgt ttttaatgtt cttttcaatc ccatctagat
     4081 aatttataga aatataaccc taattggaca tgtggtacag gatctataag ttgctgtgtt
     4141 tttttgttac tctgtatttt gttccttttg gtaaggtgaa gtgtgtccaa agagttactt
     4201 gcaacagtct ttcatgatat gaggatgccc ccgtattacc actctgatta tagttctgag
     4261 ttctttgatt tactcatgct gcatgacaaa atgtttacta ataacaattc attataaagt
     4321 tatatccctc tttacatcac ttatctttct cactgaggtt cattcactgg aatttactca
     4381 cgcaatctca gtagagtaca acgtagatac agaacctagg agagtcaaca tctggaggat
     4441 tttagtcttt cttacacata tgtgtgattt taaacgaata ttctcagacc acaggaaact
     4501 cttcatcccc ctgttgttta ccagtaacag tatatcacag acctttccaa atgtttgtat
     4561 atgtaatcag atgtacattt atattgaaaa acaaatgaga tggacttaaa gagcacatcc
     4621 tgataaatac tttctctctc acctgtacta tatttctatt agactaaagt tatgtgattt
     4681 tttttttaca ttttttcaga tgactagcaa ttttgatagt ttataagata atgcaaagaa
     4741 ctttctctga caaactaact gcagtaacag aaacctttct tttcagttac tctttttcaa
     4801 gaatgaaaga ttattataca aaaaattgta tactacttga tggaaccaac tttgtacatc
     4861 ttggccatgt cactggtcat tgtgtgaaat aaagataatc tggataatga ctattagtcc
     4921 aatgctaaga aacatgatct ttgctcatta aagagctaaa atgtttattg ctgttttgtc
     4981 tttctttttt ctaaaaaaag aaaaaaaaga aaaaaaggaa aagaagaaca aagaaacatg
     5041 actgtctcaa agagtaattt ttctagatta gaccagtcag gtttttgaag acatataggt
     5101 aacttccaca gaaaacacaa acatgtattt aaaggcaagt ctcatctaag atgaaactca
     5161 taaaaattat ttaatgtttg ttatgaattt aaaagctcca gaaatccatg aaaaaaagaa
     5221 agattagctt tgtgttgtta aatatctctt aggtacagat gttgtagaaa taaagtgcca
     5281 attgttttca gttagttaga aaaacaagtt ccaattctag tcatcaagga aaaggtctat
     5341 tgaaaagaaa tttgcaatat ttcatggaat acaaataact aggttcataa aatgggggtg
     5401 attaaactac cataaagtaa tgaatagaaa gttgccatgc taagagaatg taaaagtgct
     5461 gagcaagata ttaaactgac actaaaagat aaaaatctat ttcaaggaat ttatgtgatt
     5521 ataatccccc ctcccaatta ttatctatca aagaaatgta ggtattcctc cataattaaa
     5581 aaaaagtctc tgttagaagc aaaagtcttc aaaatccaga gtgatctaca tacagctttt
     5641 gactgaggtg gttatgcttt tcaactgtga actgccagtg tacacaaata caaataattg
     5701 tttagcatct gaagtattgt tcaattggaa cactgtattt tactgtatta attaaacaaa
     5761 acacaagaac agaatagaaa tgacaacaaa ataagatatg aatgaatact ttcatatgca
     5821 ttgcttcatt tggcaaaacc aaacattcta ttaaatagta tgttttaacg atggccatat
     5881 ttatatcatc tacctaccaa tctattgctc tgcatttatc aatctgccta tatatagcat
     5941 aactgtcacc ctctttatat taatttagga aaatgattga ggaaaacaaa gcatagccct
     6001 attaattatt ttctctaaat tagggtagaa taactttatg gaagataaag cagcctaggt
     6061 ttaattgttg ttcaccagtc acgtgataaa gtcattgtga atgagtgtta gttgctagtt
     6121 ttataattgc agtctctagt ttctgaatga aaaaggatgc cccacattat acagaacatt
     6181 aagaactgtt tttaaagctg aaacataatc tttatatgca agaaaaataa atactgttac
     6241 tcaattttag caaatatgtt taaaatgtat ttatttagat aattttcttt gaaaaacaat
     6301 gtaagtaaca aaattaaaat aatatttttg gcattatttg ttaattcaga ttttcctgcc
     6361 tccgattaaa aattaggcaa agctgcttat aatttataca aagattacac taaaatgaac
     6421 tttatttcat tgttttgctg ttatataaaa tttttaccaa atgtttacca agagattaat
     6481 gttttagagt atgaggatag aaaaataaaa gtgacagatt tcatagtttt aaatctctac
     6541 tgaacatgtt gactatgaac aaaatctaat tggctaccac agatatataa caaagtttat
     6601 tactaacttt atgcaaattg gaaaataatt tgctaaagca ttaaaaatgt cagatttgta
     6661 gacgtcttgc tggcaactca aagcagaaaa tctgagtata tattatttta ttcctcattt
     6721 caaatataaa agttgaccaa agtgatgctt tatttccgac ttcctgatct gtatgtgaaa
     6781 acagcaacat ttttttaaaa attatcaagt atatataata ttaaaaagag gaaaacagat
     6841 ggcactaatt ttcttacagg agtgaagtgc tgaaactaaa ttgttacatc tgtaacaaca
     6901 gttatttcac ctcttgccac actcatcctg acatacgtat taatatcaaa aaatgataca
     6961 aagaatatga gaaatgtgtt ccagcattaa agacactact acatagaaat tactgggatt
     7021 cttttctttg gcgagtttca ctgaaatagt ttcacaggat gaaatgatga attgaattct
     7081 taagaagctg tcaaatatgg cagtcttttg atgttagtaa ttttgttttc ttctgtgtta
     7141 ttggttcaaa gtactggcct tttccttcat ttccagtaat tagttgttat agactatcac
     7201 tttttaatgt gagtggaaat tagatgattt ggttatactt gtgaaaacat gcttccaaat
     7261 aaattcaatt aatgggcact tccctgctga gattttgtta tcattcattt gattatcttg
     7321 tcaagcttta ctgttacgac aaatgtttta gctttctggt caatagacta agggaatttc
     7381 tccagtagcc ttatacctct cctgcacttg atctgatagc aacatacact tcaaatttcc
     7441 atcagaagtc tctaaagaaa tttcctactg ctatgaaaca tttggtggcc aacttgattc
     7501 tttttcataa gcattctgat ttgattttat tctggcacgt tgctgtattt ggatgcaggc
     7561 cagaaatcaa ttccatttga taagggttgt ggagaataca gactggaata accgtgtgca
     7621 gggcttaagt gccttttcct cctgagattt tatttataaa acttagcttt tttaaatttt
     7681 cagattcaga taattcaatt taaagagtac aataggatga ctatattata aattaccaat
     7741 aaagaaagtt tatttggaca tttttacatt taatttgatt tttaaaataa tatcagatgc
     7801 atctaaatgg cttccgggaa agactgaggt aaatttttga aagtgacaat tatataggca
     7861 tctgacttga tggtacttaa gaataaaaat ttaaatctct agcaaacttt aaaaattgtc
     7921 caaatagtaa gtttaatttg aaagaagtct gaagctatta gagaaaatca tgtactagat
     7981 aggtatatat ttttttctag tcagctatta cgtacaccac ataatatata taccctatat
     8041 actatatggt ttatataaca tttataatgg ccctttcaga cccatatact tacaaatgac
     8101 attgtataac tacctacata tgtattaatg tgacagaatt tagtccttta tggaaagtca
     8161 aaccaaattg ctagcagttt tgagttgaaa tgaatgataa caattaaagc ttttaagaaa
     8221 ttggtaaagg taccgtaaca aaaactcagc agataaattt aaaagagagg gaccattttt
     8281 gaatgccatt ttcagagttg acatctaaac tagatggtgt gtttaaaagg attttagtgg
     8341 ttttgattca tcctttctct gcattcatat catttagcaa attaatggga catatgtcat
     8401 ggtttttgta aaaaagacca ttgggttgtc atgattcagt ttgtgtttgg acatctatgg
     8461 ctttcattaa aaacgtgttt attatcatga ataaatatta ccatgttttc caagttgtgt
     8521 tgactatttt taaactcaga aataatttct tctctatttg tctcacacaa aaaggttact
     8581 aagataagtg ttttcttaca aagtctttgt attgcttcca cactgctgac agttctgact
     8641 tgatagaaac aatgacattt tatgatcttt tcacctcttg gtgaatgcag ggcaagaatt
     8701 tagagaatag atttggggag ctggacacaa cttttcagaa aagagagagg aaagtgggag
     8761 aaggaaaaag aaaagaatgg aaaaaggagg aagtgagaga gaacaaaaag gaagaaaaac
     8821 ttctcaattt taaattaatg actttttgca tccaaatagt gctttaaata gagatgtgtg
     8881 acagtttttt aaaaagaagg gcaaaggaat ttaaataaat ccctttaaat cataaatcta
     8941 ttattcttag agaacaaaat taataatatc taggaagata tcttaaattt aattcttgat
     9001 ccaagtcttg atcaaatcat gtcactggaa acaattaaaa ataaaatcta tactgaaata
     9061 tttatggttt tgtactatct aatcaataat gatattggta ggaagactat gggaagtaag
     9121 ataatctctc atctgtatat agcccaagat aagaatacag caaagaggac atcatataat
     9181 ttcctcatac atagcctaaa gataaatgtt tccaatggct gaataaaaga attagcattg
     9241 ctttacaagt ctctgtggtc acatatggcc atgggcacct aataaattaa acagttaaaa
     9301 aacatattct aacaatttca ctttccataa aagggttttg atgttttgta aaatttggtg
     9361 ttgtttgata tgattacttt ttcactagtt ttgtatttcc ttatggtttg taggatgtta
     9421 tttctgagtt caaaaaaagt ttcaaatata ctcgtgaatg ttcatgtgaa agcagtattt
     9481 tctaatggaa tgctataatt tgaagtactg tctcaatttt ggagagttga ttgactatct
     9541 agttaaaact tttctatctc ccctgaaaat actcaacttt gctctcagag ctgccttcct
     9601 ctgcactaca ggggatcttc ttaccagtca ctgtggctcc tgtttccatg gcctatccta
     9661 ttactagtta tgacctcttc tccaaggggg taaagttgtg ctgaaagtct taaaaggtga
     9721 tctaaccaac tggtgccttg ggaattggct tcctctgctc ctcaggcagg ttagaagcta
     9781 gtgaacatca atcaagcaat gaaagaaatt agtcatttct tctttcaaca aatgttgtct
     9841 actatgggtt aggcaacata atgggtgtta aacacgtggc caattctatg acttactaac
     9901 atacaactat tgtcaagttt ggaataggtc caaacacatg gactgattta gaccactttc
     9961 tatgctatac tctgtagcaa atcactgtac aattccttgt tttgtctatt tcacatgtct
    10021 caaaatagta aatacaaatc aatatttctt gagttcaaaa aaaattagtg aattctattg
    10081 agccagttca ccagttaagg tatgtagcag acatctttcc aaataataaa tacagcctat
    10141 ctgaagtcat gctatagtat gccaattcaa atttcatgga atatgaaatt tccagcataa
    10201 atctcatctg atccagcccc ataagtggtt ccttaatcct ttgccacaac ctcaacatgt
    10261 aattgtgaag cctatcattg aacactgcca tgggtctgaa ttctaaaaca gctggctgca
    10321 ttttggacta tttgggatgt cttctttata ttgatttctt gctctttttt tttcttttta
    10381 tagccacacc gtcttcatcg cctgaattga tctcaaatat ttaaagatgg ctttcatatc
    10441 ttccatgagt tattttctcc tgaaagcaaa aaatcccact tgaaatacaa aatagttcag
    10501 tcttttcaca attctaatca ctgttctcca aatttgctcc caagcctaag tttcttagaa
    10561 agtttaatgt ctaggattga ccataataaa cacttacgta tagttcatct ctgcctaaca
    10621 gagctccact atcaacttcc tcattacaca aagtatattt attttaatat aacttgcaat
    10681 actattacat tctttgttgg ccatagcata ctagtaattc agattgagat gtactttcta
    10741 ctaaaacttt aaaaattcta aatgcactat tgttaagcaa attctgtctt tctactgccc
    10801 ttgatcattt gagttttatt gtatgttcag gatattaaat actattaaat tctacctcat
    10861 taggtacagc actacagcct atggagacct atctatccac ctcagagtaa ggaaatcaac
    10921 aaagatgata aacttctatc ttcatccaag tcactaacaa aaactaccat gcagaatcag
    10981 gttgaggata aatctccaga ataatagtaa tagagacagt gtgtaaagta ctgacaaaag
    11041 caaaagatga caattactaa gcccttttat gttccagatt ctgtatcaca ctccacaaca
    11101 aactcccaca taccaccctc gaaatatcta ctaatacttg ttgatgtgct tattcactaa
    11161 ttcgttgttt aaaagattat tattaagcat ctattatata tgagttactg agctacttgt
    11221 taatgataga aaagtgaata aaatgtggct ccgtttctca cattactagc agccaactga
    11281 ttactcttcc tcttttctac acacacagca caaaagacac aatcagatgt atttgcagga
    11341 tatatttttt tcccatgtaa aacatttgcc tgacatcata gtcatcttta gtaagaaaac
    11401 tgggataatt actctaaact ttctatttgg gaatttctgg gttcctggtg cattatatta
    11461 aatgttagaa gatatagaaa aataattctc acaccaatca cctgtcataa ttaagatgtg
    11521 aatttaggcc agtcaagtgt tgtcttaaag tgggattcct tgtatgaaaa agtaatacat
    11581 acataagata aaacaaaata ttttaaagtg aaaagtaaac atctctctta ctacagattc
    11641 taatccctta gtctttttcc ccagagacat tgactctcac caatttgcat ataaatctct
    11701 acaaatatgt cattcatgtg taaacatgta aaagtgtatt gtctgtgtat gtacgtgggg
    11761 gcactttata tatatatatg tatgtatata tatgtgtata tatatatgta tgtatatata
    11821 tgtgtatata tatatatgta tatatatata tatacatata tattcttttt ttaaatttat
    11881 ctggaaatgt tccatgtgag gatactacat gtcagctctg aggagaaaat agaagataaa
    11941 agtaagtttc aaatttctcc ttaaattcac ggctattcca atcaaaattt caacactgtt
    12001 tgtttgttta cagactttag gtgagtaagg gtcaggaata gccaagatta ttttgaatca
    12061 aaagaatcag cagcaaagac tttgtctacc gtatttcatg agttactata aagctattgt
    12121 aatcaaaaca gtgttatatc aattcagaga cagaccagtg taggataaca ttgaacccat
    12181 aaacagatct atgaagaaag gggaacatgg catataaatg gaaatacaaa tcaagagaaa
    12241 agagagatgc ctcagctaac aatatcccta tgtcatacta gacacaaaaa tcaatcccag
    12301 acaaattaaa aatataaaca taaaagcaca ttttaaatgt ctcagaatac ataggaaaac
    12361 attgtcatgt cctcgaaata gggaagaatt tattaacaag acataaaaca gcataaacaa
    12421 taaagaaaac gaataacaag ttaaaattaa aatgttaata catcagataa tgagtttttt
    12481 taaatgacta taagcacata ttgaaagtaa gtatttgcaa cacatataag caatgaaaga
    12541 ttaattaata tgagtggata gaaaattcct gtagtttaaa taaggaaagg acaaattcac
    12601 aaggaaaata gagctgatta aatactggga ataaaggaca tgtctgtttt ttccctgttg
    12661 ctgattctca caaaaatgta aggagaggac tgaaaaatat ataaacttat cagaacaaaa
    12721 agaatagaaa aagagataac aggtaagaag ttctgacaag tttttgagag agagaaatta
    12781 gataaactaa aaaaccttaa gaatataata caacctatga aatagcaaca taaatcagaa
    12841 ttagaaaagt taaaagatgc tttaataaaa ctcaagaaag aattataaat aaaagaaaaa
    12901 ataatttttt aaattaagac taaattaaag gaaatgcaga ccaaagaatg ctttaagaga
    12961 catagaaaaa ttttcaaaaa gaaatgggaa taaatagtaa aaagtagaaa aaatctaaaa
    13021 agtttcaaaa aagaaggaat aggggatgtg agctgagggg gagaggaagg gagaaataag
    13081 aaaaaaaatc aagcataagt gataagaaga tagaaaaaga agactcaata tgcatgtata
    13141 tggaaccttc gaaaaagaaa accaaaacaa ggaaacacaa caaatacaat gaaagctgta
    13201 attcaggaaa tcttttacaa tacaacagaa aaaaatttga tgtcacattt tcaaaaggca
    13261 cactgaatat atgaaataac tgatcaaaaa tgaccaacat caagacatat gctaataagt
    13321 ttaatgacta tcaaagaaat aatataagca ttcacatcta gctaaaatca ccaaatctct
    13381 taaaatgaag actcttttgt tatataatta gatcatcatc agacttcaaa gacaacgctt
    13441 tatgccagta aaaattaaag taacatacaa aagataagac gttagaatgt aagcaagtta
    13501 tttttatatt cagccataag ctaactttcc tacaagtatg aagggcatgg acaaactgtt
    13561 attggcatta aagaactcag ggaatattgt taccatgaac gcctcctgag aaatttacca
    13621 gaaaaagacc tgtagacaaa cagaagggtg acatggatat aaggactgat aatgggcact
    13681 aaataccaga gagctaacac taaatgaggg ctatatggtg gagtacagta taaacttgtt
    13741 acatgttctg ataatgaaga cacggtacag ctataagaaa tatgaaggta atagaaaaat
    13801 cataggtaaa aattgtcatt actttcaata atcatattta cagtggtagc agtgctgttg
    13861 ctactctgaa actgctttat gtgtaacaca gaatacagca aagagggata ttgtgatatg
    13921 ctaattgtat catctccacc gtctttgaga accagcactc ttaatgtgga agaaaggata
    13981 ttctgaatca gagtagaaga ggttaagtaa aaacccacct tattgtttcg attttaaagt
    14041 gtaactatca gaatgaacac tttaaaaaaa gttatgcaaa ctatgatcaa gctagtagca
    14101 atgagcatcc ttaaggacca gattgtggtt ttaaaagaac atttcttact gtaaaaaatt
    14161 caccacgcac acaaacacac atagaaatag aggtctgggg aacaaaccat agaaggtgaa
    14221 cctggaacat tttgtcaaag acttctagga atatatctaa aggacagagg aaccaataca
    14281 aagatgatct cactatctaa agatacgaca gtgtgagcat caataaggat aatttcaatt
    14341 aattgaaaat aaatccaatt taaattctaa agtgaataat aattaaaaag aaaacatcta
    14401 ccacttatgg cagataattg gaatataatt cattgttttg aaaattcata agtaaaaaga
    14461 acagttaaat attttgtcct gccattcctc caccaactat acagcaaggt agccaaatgg
    14521 tagatgagaa tttactcttt gtagaagtta tttcagctaa taaaagaagg aattgttgta
    14581 ttataatttc agttgtcact tgttttattg ctcccaatga atcagtggat ctaggtatta
    14641 agcatcacaa aattagaaat aatgagttat catgtgtctc ctggatagat gtacacaaga
    14701 ttacctcaga aaaggcttgc acccaaaacc aaacctgtgt atgatggaag tctctaggtt
    14761 caactgtcag aaaatacata ggtacaggaa gtacatcaaa gaagcatgtt aaattatacc
    14821 atggatatga aatcagaaaa attcacacga tttaaaattt tcccagttta attttcttgt
    14881 atcctcaata aataaattgc aagaaaaaaa acacagtgag aaaatatgta gatttttaaa
    14941 aacttacaaa tactttttaa aaaagttctg cttcaggaag aacgattcag taataaaatt
    15001 ataaaggaca gcaagatggg gtaacctaaa atagtgttga tagatcacag aggaaataag
    15061 ctgtgactga gatggtgcat gtggaggttt tttttgcagt ggcttgcaaa gtgctatctc
    15121 ttaatctggg tgttttttac ctgagccttt tccttatgat gatgttttaa accaaacatt
    15181 tacttcatga tgtttttctg tacctggatt atattttaca ataaaataaa taggattgta
    15241 ataaaattaa taatacacga caatgttgca gacttaaaga acatgagttt attaattgaa
    15301 aggacccgcc aactgcccag tgtaatggat gaaaaaaaca cctgtgaaag cataccatca
    15361 caaaatgtca tgacttcaga aaaaagaaga aaagtttcaa agcttcctca gaggaacaaa
    15421 aggaattctc aacgtcagct ctggaagcta gaagaaattg gataatattt ttgagaaaga
    15481 attatgatca acctatagaa ttttatattc aaacaaaata taagttcact gtatcagtcc
    15541 acaaaggcat agattcaggg gtgagaggaa ataaaaaacc aggattgctg ctcaggagac
    15601 tagagaacag ctcagagtgt gaagctgggg agttgtagaa agaatatcta tacaataaaa
    15661 ttaaaactga cgtatttcat catgtggaaa aaagtgttat ttgtatagat tttacaattt
    15721 tttttgctaa atttggaaag aacatagaaa actacacaac aaaatcaaaa ctattaatgc
    15781 agagaaaaag agaaagtact acaagaaatg gaatgtaatc tttggatata tatttgtgca
    15841 catgaatgta agtacagata attcttccaa atagaaagtt tggaaaaagc atattttaag
    15901 gtttttatct accaatttac taattgattt aaaaagatgt tattcaaatt tacattccac
    15961 aaacagtgta ttaaagtatc tatttcccta ccctctaacc aaaaatcagt gtatcaattt
    16021 tttatatttt ccatttcaat ataaatgtaa tatcattatt gtattaattt gcatgtttaa
    16081 attgaggtaa aacatctttt cttatgctta taagccattg tatttctttt tatattagat
    16141 gtattccttt actttgtata ttttttctat tcgtttatta atctcttctt atgaaattat
    16201 aagagttctc tatagacaaa taaaatcagt acttttggtc a
//
