LOCUS       NM_018096               5193 bp    mRNA    linear   PRI 23-SEP-2024
DEFINITION  Homo sapiens notchless homolog 1 (NLE1), transcript variant 1,
            mRNA.
ACCESSION   NM_018096
VERSION     NM_018096.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Loevenich,L.P., Tschurtschenthaler,M., Rokavec,M., Silva,M.G.,
            Jesinghaus,M., Kirchner,T., Klauschen,F., Saur,D., Neumann,J.,
            Hermeking,H. and Jung,P.
  TITLE     SMAD4 Loss Induces c-MYC-Mediated NLE1 Upregulation to Support
            Protein Biosynthesis, Colorectal Cancer Growth, and Metastasis
  JOURNAL   Cancer Res 82 (24), 4604-4623 (2022)
   PUBMED   36219392
  REMARK    GeneRIF: SMAD4 Loss Induces c-MYC-Mediated NLE1 Upregulation to
            Support Protein Biosynthesis, Colorectal Cancer Growth, and
            Metastasis.
REFERENCE   2  (bases 1 to 5193)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   3  (bases 1 to 5193)
  AUTHORS   Romes,E.M., Sobhany,M. and Stanley,R.E.
  TITLE     The Crystal Structure of the Ubiquitin-like Domain of Ribosome
            Assembly Factor Ytm1 and Characterization of Its Interaction with
            the AAA-ATPase Midasin
  JOURNAL   J Biol Chem 291 (2), 882-893 (2016)
   PUBMED   26601951
REFERENCE   4  (bases 1 to 5193)
  AUTHORS   Scherl,A., Coute,Y., Deon,C., Calle,A., Kindbeiter,K.,
            Sanchez,J.C., Greco,A., Hochstrasser,D. and Diaz,J.J.
  TITLE     Functional proteomic analysis of human nucleolus
  JOURNAL   Mol Biol Cell 13 (11), 4100-4109 (2002)
   PUBMED   12429849
REFERENCE   5  (bases 1 to 5193)
  AUTHORS   Stanchi,F., Bertocco,E., Toppo,S., Dioguardi,R., Simionati,B.,
            Cannata,N., Zimbello,R., Lanfranchi,G. and Valle,G.
  TITLE     Characterization of 16 novel human genes showing high similarity to
            yeast sequences
  JOURNAL   Yeast 18 (1), 69-80 (2001)
   PUBMED   11124703
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BP244661.1, BI911995.1, BC012075.1, BQ070427.1, HY320157.1 and
            AC022916.11.
            
            On Nov 24, 2018 this sequence version replaced NM_018096.4.
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (a).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.22141.1,
                                           SRR1803613.4197.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000442241.9/ ENSP00000413572.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-26                BP244661.1         13-38
            27-46               BI911995.1         20-39
            47-533              BC012075.1         32-518
            534-565             BQ070427.1         278-309
            566-2573            BC012075.1         551-2558
            2574-2601           HY320157.1         32-59               c
            2602-5193           AC022916.11        95716-98307         c
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="17"
                     /map="17q12"
     gene            1..5193
                     /gene="NLE1"
                     /gene_synonym="HUSSY7; NLE; Rsa4"
                     /note="notchless homolog 1"
                     /db_xref="GeneID:54475"
                     /db_xref="HGNC:HGNC:19889"
                     /db_xref="MIM:620924"
     exon            1..46
                     /gene="NLE1"
                     /gene_synonym="HUSSY7; NLE; Rsa4"
                     /inference="alignment:Splign:2.1.0"
     CDS             29..1486
                     /gene="NLE1"
                     /gene_synonym="HUSSY7; NLE; Rsa4"
                     /note="isoform a is encoded by transcript variant 1;
                     Notchless gene homolog; notchless protein homolog 1"
                     /codon_start=1
                     /product="notchless protein homolog 1 isoform a"
                     /protein_id="NP_060566.2"
                     /db_xref="CCDS:CCDS11291.1"
                     /db_xref="GeneID:54475"
                     /db_xref="HGNC:HGNC:19889"
                     /db_xref="MIM:620924"
                     /translation="MAAAVPDEAVARDVQRLLVQFQDEGGQLLGSPFDVPVDITPDRL
                     QLVCNALLAQEDPLPLAFFVHDAEIVSSLGKTLESQAVETEKVLDIIYQPQAIFRVRA
                     VTRCTSSLEGHSEAVISVAFSPTGKYLASGSGDTTVRFWDLSTETPHFTCKGHRHWVL
                     SISWSPDGRKLASGCKNGQILLWDPSTGKQVGRTLAGHSKWITGLSWEPLHANPECRY
                     VASSSKDGSVRIWDTTAGRCERILTGHTQSVTCLRWGGDGLLYSASQDRTIKVWRAHD
                     GVLCRTLQGHGHWVNTMALSTDYALRTGAFEPAEASVNPQDLQGSLQELKERALSRYN
                     LVRGQGPERLVSGSDDFTLFLWSPAEDKKPLTRMTGHQALINQVLFSPDSRIVASASF
                     DKSIKLWDGRTGKYLASLRGHVAAVYQIAWSADSRLLVSGSSDSTLKVWDVKAQKLAM
                     DLPGHADEVYAVDWSPDGQRVASGGKDKCLRIWRR"
     exon            47..190
                     /gene="NLE1"
                     /gene_synonym="HUSSY7; NLE; Rsa4"
                     /inference="alignment:Splign:2.1.0"
     exon            191..408
                     /gene="NLE1"
                     /gene_synonym="HUSSY7; NLE; Rsa4"
                     /inference="alignment:Splign:2.1.0"
     exon            409..488
                     /gene="NLE1"
                     /gene_synonym="HUSSY7; NLE; Rsa4"
                     /inference="alignment:Splign:2.1.0"
     exon            489..565
                     /gene="NLE1"
                     /gene_synonym="HUSSY7; NLE; Rsa4"
                     /inference="alignment:Splign:2.1.0"
     exon            566..663
                     /gene="NLE1"
                     /gene_synonym="HUSSY7; NLE; Rsa4"
                     /inference="alignment:Splign:2.1.0"
     exon            664..856
                     /gene="NLE1"
                     /gene_synonym="HUSSY7; NLE; Rsa4"
                     /inference="alignment:Splign:2.1.0"
     exon            857..992
                     /gene="NLE1"
                     /gene_synonym="HUSSY7; NLE; Rsa4"
                     /inference="alignment:Splign:2.1.0"
     exon            993..1039
                     /gene="NLE1"
                     /gene_synonym="HUSSY7; NLE; Rsa4"
                     /inference="alignment:Splign:2.1.0"
     exon            1040..1242
                     /gene="NLE1"
                     /gene_synonym="HUSSY7; NLE; Rsa4"
                     /inference="alignment:Splign:2.1.0"
     exon            1243..1402
                     /gene="NLE1"
                     /gene_synonym="HUSSY7; NLE; Rsa4"
                     /inference="alignment:Splign:2.1.0"
     exon            1403..1473
                     /gene="NLE1"
                     /gene_synonym="HUSSY7; NLE; Rsa4"
                     /inference="alignment:Splign:2.1.0"
     exon            1474..5193
                     /gene="NLE1"
                     /gene_synonym="HUSSY7; NLE; Rsa4"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1841..1846
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NLE1"
                     /gene_synonym="HUSSY7; NLE; Rsa4"
                     /note="hexamer: AATAAG"
     polyA_site      1854
                     /gene="NLE1"
                     /gene_synonym="HUSSY7; NLE; Rsa4"
                     /note="major polyA site"
     regulatory      2555..2560
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NLE1"
                     /gene_synonym="HUSSY7; NLE; Rsa4"
                     /note="hexamer: TATAAA"
     polyA_site      2573
                     /gene="NLE1"
                     /gene_synonym="HUSSY7; NLE; Rsa4"
                     /note="major polyA site"
     regulatory      2858..2863
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NLE1"
                     /gene_synonym="HUSSY7; NLE; Rsa4"
                     /note="hexamer: ATTAAA"
     polyA_site      2880
                     /gene="NLE1"
                     /gene_synonym="HUSSY7; NLE; Rsa4"
     regulatory      4030..4035
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NLE1"
                     /gene_synonym="HUSSY7; NLE; Rsa4"
                     /note="hexamer: ATTATA"
     regulatory      4048..4053
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NLE1"
                     /gene_synonym="HUSSY7; NLE; Rsa4"
                     /note="hexamer: AATACA"
     polyA_site      4051
                     /gene="NLE1"
                     /gene_synonym="HUSSY7; NLE; Rsa4"
     polyA_site      4069
                     /gene="NLE1"
                     /gene_synonym="HUSSY7; NLE; Rsa4"
     regulatory      5162..5167
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NLE1"
                     /gene_synonym="HUSSY7; NLE; Rsa4"
                     /note="hexamer: AATAAA"
     polyA_site      5193
                     /gene="NLE1"
                     /gene_synonym="HUSSY7; NLE; Rsa4"
ORIGIN      
        1 ctctttctcc tccacgtggg gacgcaggat ggcggcagca gtgccggacg aggcggtggc
       61 gcgcgatgtg cagcggttgc tagtgcagtt ccaggatgag ggcgggcagc tgctgggttc
      121 cccgttcgac gtgcccgtgg acatcacccc ggacaggctg cagctcgtgt gcaacgcgct
      181 actggcccag gaggatcccc tgccactggc tttctttgtc cacgatgctg agatcgtctc
      241 ctcactgggg aagacgttgg agtcccaggc agtggagaca gagaaggtcc tagacatcat
      301 ctaccagcca caggctatct tcagagtccg ggctgtgact cgctgcacca gctccttgga
      361 gggtcacagt gaggcagtca tttctgtggc cttcagccct acgggaaagt acctggccag
      421 tggctctgga gacaccaccg tgcgcttctg ggatctcagc acagagacac cacatttcac
      481 atgcaaggga cacagacact gggtccttag tatatcctgg tctccagatg gcaggaagct
      541 ggcctcaggc tgcaagaatg gccagattct cctctgggac ccaagcacag ggaagcaggt
      601 gggcaggacc ctcgctggcc acagcaagtg gatcacaggc ctgagctggg agcccctcca
      661 tgcgaaccct gagtgccgct atgtggccag cagctccaag gatggcagtg tgcggatctg
      721 ggacacaact gcaggccgct gtgagcgcat cctcaccggg cacacccagt cggtcacctg
      781 tctccggtgg ggaggggacg ggcttctcta ctctgcctcc caggaccgca ccatcaaagt
      841 ctggagagct catgacggtg tgctgtgccg gactctgcaa ggccacggcc actgggtgaa
      901 caccatggcc ctcagcactg actatgccct gcgcactggg gcctttgaac ctgctgaggc
      961 ctcagttaat ccccaagacc tccaaggatc cttgcaggag ttgaaggaga gggctctgag
     1021 ccgatacaac ctcgtgcggg gccagggtcc agagaggctg gtgtctggct ccgacgactt
     1081 caccttattc ctgtggtccc cagcagagga caaaaagcct ctcactcgga tgacaggaca
     1141 ccaagctctc atcaaccagg tgctcttctc tcctgactcc cgcatcgtgg ctagtgcctc
     1201 ctttgacaag tccatcaagc tgtgggatgg caggacgggc aagtacctgg cttccctacg
     1261 cggccacgtg gctgccgtgt accagattgc gtggtcagct gacagtcggc tcctggtcag
     1321 cggcagcagt gacagcacac tgaaggtgtg ggatgtgaag gcccagaagc tggccatgga
     1381 cctgcccggc cacgcggatg aggtatatgc tgttgactgg agtccagatg gccagagagt
     1441 ggcaagtggt gggaaggaca aatgcctccg gatatggagg agatgagacg gcccgaagtt
     1501 ctctctgacc cccacctcga ctcggcctct gccagctgcc ttccctgcca gagaacaaag
     1561 gctgagatgg cagtgcacac accctcccca ccagtgggga cctgagaatg cgtgtggcct
     1621 gctgtcctcg atagaccgga atggggtttt cccacagatc cccgcctgtg gcacacccca
     1681 gagccagaaa tcgaaggtca caggaagttg tcactgaact tggcccgtgt ctgctactct
     1741 gtaccttgct ggtacagaca ggggtggtgg gcagccaggc tctatgagtg ggcccctagt
     1801 gtcagctctg tacagggtca gatcccaggt tctatgacca aataagtaac ttaagttttg
     1861 tgtgttgggt tctaattcct tgtcctagaa tccccatgac tcaatcaagg actgtgctaa
     1921 atgagattgt ccagcccccg cccttgcact ggactacgcc aaaaccacac tgaccaggca
     1981 cttgccttcc ctctcttccc ccgtgttggt aagagagagg ccagttgtga tagtggccaa
     2041 ggagaatcta gggctgtatt gttgtccact gcagtaggca ccggccacat gtgactgctg
     2101 gcatgaaata gaagtgcagt tcctccatcg cactgggtaa ggcctccagt attggacagc
     2161 acacagaaag gttttcatca tcaagagagt tctgctggtc agccctgctc caggggatgc
     2221 ctctgccttc gcatagcaca ctgcttgagg ccctgccagg caccaagcac tgccctgggc
     2281 ccatgggata gagcggggaa ggtgatggct cttccagagg attccctcag atggggaggc
     2341 agcagtatga gctctgagca gaagtgggta ttgttgatac agaggaagtt ctttgccacg
     2401 agaactttca agcagtgaaa ggaattccca tcaggactca gaccccaggc cgagatcttg
     2461 ccctgaatgt accctgcctc tgctttctcc tgcatcccat gctaagcagg gtcatggtct
     2521 gaactactca gattggattt ccaaaccatc cttgtataaa ctgctcagaa ctagcatctt
     2581 gctccttctt accctcctcc acccttgtct gtgcttttgg gttttgcgcg tcacctagct
     2641 gtggtgcttg gcctccacct tgcacatatc tgcagtgagg ctacataaga agcgaacaaa
     2701 actatcagcc actgagctcc ttggtgccag ctgtttgtct gctgtgctct cagactgcac
     2761 caggcctgga aggttctcaa gctccaaaac ttttgatgag gaaacaacat aagaaatgat
     2821 tagcccatgc cctcacatct agtaagagaa agctgggatt aaaccaggcc ctgccccaaa
     2881 gcttattgtg cactttgtac cacatccctc cgtccagagc tcaagctagt ttgacagttc
     2941 atgggccacc acgtctgtcc atgacagcta tttctgtatt tttctggtgt aagagccttt
     3001 tagtcttaga gcaccagtct ccctgttccc ctaccccgcc ccactgggga aggggtgatg
     3061 gcagcatcct attgcctagc tgcctgtttt ctatctcccc acccatgtag actctgtcct
     3121 ggtggaagct aggaggtggg tgccagccag cccatctgag gctgtctgca gcataaagca
     3181 gactttaggg tgctggatga cctgcagttc tttaatgagg gacctagacc cagcccagat
     3241 ttggaggagg agggagtcta gcagctgtgg gcagtggctg agcctggcat ggcctggcct
     3301 gagatgcctc caggtgaatg gggcccctat gtagccttgg ccccagccca ggggtggggt
     3361 gcccatacca ctctgccaga ccccgggccc gcagggtgct ggtggtatgg taggtctcat
     3421 ggctctggtt tctgagggcc tggggttgaa gctgaaagta ggtgaggggt gtcctggggc
     3481 cctccccctt aacagggaaa taggtattcc agatcctcca gcctccgcat ctcctccggc
     3541 ccgatggaca cgatcttttg ccggggttcc ttgcgccgca cggtgatctg gatggggtag
     3601 ttctcaggaa agctgctgaa gtcagtggcc agggttgcaa actgaagtga aacaagaaaa
     3661 cccttcagag ttccttccca gatctgaacc tggctctctc tctgtctcta tcaccccttt
     3721 tttttctgtc tgctgactca gaccttaaac agccttatcc aacctagcca ttgacgcagg
     3781 aaacaggtgc aaagatgcat gactcaagcc tgtacctcct cattatggga tatttccaac
     3841 caaagggctg tctgtactca ggcctctata cccccatacc cctaccccat acccaggctg
     3901 ttcccttctt cgtccccctt tagcctcttg tcttccctat ttctttcaat gcccttgact
     3961 tcccagccta ctcacaatca tgggcactga attgcagagt ctaaaaacca atggctttgg
     4021 aaggggaaca ttatagtgat gttgaaaaat acattcgttt gcattcctag tgggccccag
     4081 agtggcttaa tccttaaacc tcccagaagc atgcattctg gaattgatgc ttcttgaccc
     4141 aggcagaagg aaagctagcc agatgagtca tgtgggcata ggggtgttcc caacctccaa
     4201 agccctggtt ccctggcttt ggacttctct ccacccccaa ggcccctctt ttcccagggc
     4261 tcactttgta gggcttgcac cacttcccca ccccagctgt gttggctgca cggacactga
     4321 ggcagtaaca cgtgttaggc tttagctcca tcagcttgac agtagtgccc aaaatcttgt
     4381 tgaagatcca tggacgattc tttgcctcgg gcaactcatt ctcctgtgtc ttggccacct
     4441 cctgtaacag gagctggtag aaactgaccg gctgcttgcc caaggcgtag gtccaggaaa
     4501 tctaggggaa gagagagccc cgaggcttcc tcagatatgt cctgtccttc ctgtcccttc
     4561 accctcccaa ctggggtcag gacgtcccat gcccatcccc tgctcctgct ggggcccagg
     4621 caggtcagat ggaaggtgca aggtactgca gcactgagtg tggaacccct gccccgaagc
     4681 atgtactact ctcctgggca gccccaccca ctttagggta caccctcatt ccatatttct
     4741 tatacgccag gggtcctcaa cccttgggcc accaactgat agcagtccat ggcctgttag
     4801 gaactggata gcacagcagg aggagattgg tgggcgagca agcgaagctt catctgtatt
     4861 tacagctgct ccccacggct cagggtacca cctgagctct gcctcctgtc agatcagcag
     4921 aggcattaga ttcccatagg cacgtgaacc ctattgtgaa ttgtatggga gggatctaag
     4981 ttgaatgctc cttatgagaa tctaatgcct gatgatctgt cattgtctcc catcaccccc
     5041 aaatgggacc ctctcgttgc aggaaaacaa gctcagggct cccacagttt ctacattatg
     5101 gtgagttgta taattatttc attatatatt acaatgtaat aataatagaa ataaagtaca
     5161 caataaatgt aatatgcttg actcatcctg aaa
//
