LOCUS       NM_033120               2182 bp    mRNA    linear   PRI 05-APR-2024
DEFINITION  Homo sapiens NKD inhibitor of WNT signaling pathway 2 (NKD2),
            transcript variant 1, mRNA.
ACCESSION   NM_033120
VERSION     NM_033120.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Ke,S., Pan,Q., Wang,C., Su,Z., Li,M. and Liu,X.
  TITLE     NKD2 Trigger NF-kappaB Signaling Pathway and Facilitates Thyroid
            Cancer Cell Proliferation
  JOURNAL   Mol Biotechnol 65 (11), 1846-1856 (2023)
   PUBMED   36820951
  REMARK    GeneRIF: NKD2 Trigger NF-kappaB Signaling Pathway and Facilitates
            Thyroid Cancer Cell Proliferation.
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Wu,B., Woo,J.S., Vila,P., Jew,M., Leung,J., Sun,Z., Srikanth,S. and
            Gwack,Y.
  TITLE     NKD2 mediates stimulation-dependent ORAI1 trafficking to augment
            Ca2+ entry in T cells
  JOURNAL   Cell Rep 36 (8), 109603 (2021)
   PUBMED   34433025
  REMARK    GeneRIF: NKD2 mediates stimulation-dependent ORAI1 trafficking to
            augment Ca(2+) entry in T cells.
REFERENCE   3  (bases 1 to 2182)
  AUTHORS   Wang,Y., Dong,T., Wang,P., Li,S., Wu,G., Zhou,J. and Wang,Z.
  TITLE     LINC00922 regulates epithelial-mesenchymal transition, invasive and
            migratory capacities in breast cancer through promoting NKD2
            methylation
  JOURNAL   Cell Signal 77, 109808 (2021)
   PUBMED   33045317
  REMARK    GeneRIF: LINC00922 regulates epithelial-mesenchymal transition,
            invasive and migratory capacities in breast cancer through
            promoting NKD2 methylation.
REFERENCE   4  (bases 1 to 2182)
  AUTHORS   Cao,Z., Singh,B., Li,C., Markham,N.O., Carrington,L.J.,
            Franklin,J.L., Graves-Deal,R., Kennedy,E.J., Goldenring,J.R. and
            Coffey,R.J.
  TITLE     Protein kinase A-mediated phosphorylation of naked cuticle homolog
            2 stimulates cell-surface delivery of transforming growth
            factor-alpha for epidermal growth factor receptor transactivation
  JOURNAL   Traffic 20 (5), 357-368 (2019)
   PUBMED   30941853
  REMARK    GeneRIF: NKD2 phosphorylation by PKA promotes cell-surface delivery
            of TGFalpha and increased EGFR activation.
REFERENCE   5  (bases 1 to 2182)
  AUTHORS   Yang,H., Li,G., Cheng,B. and Jiang,R.
  TITLE     ZFAS1 functions as an oncogenic long non-coding RNA in bladder
            cancer
  JOURNAL   Biosci Rep 38 (3) (2018)
   PUBMED   29678899
  REMARK    GeneRIF: The experiments in vitro suggested that knockdown of ZFAS1
            repressed bladder cancer cell proliferation via up-regulating KLF2
            and NKD2 expression, and inhibited cell migration and invasion via
            down-regulating ZEB1 and ZEB2 expression.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2182)
  AUTHORS   Adamowicz,M., Radlwimmer,B., Rieker,R.J., Mertens,D.,
            Schwarzbach,M., Schraml,P., Benner,A., Lichter,P.,
            Mechtersheimer,G. and Joos,S.
  TITLE     Frequent amplifications and abundant expression of TRIO, NKD2, and
            IRX2 in soft tissue sarcomas
  JOURNAL   Genes Chromosomes Cancer 45 (9), 829-838 (2006)
   PUBMED   16752383
  REMARK    GeneRIF: NKD2 represents a candidate target of 5p amplifications in
            soft tissue sarcomas and might play a crucial role during the
            progression of this disease.
REFERENCE   7  (bases 1 to 2182)
  AUTHORS   Creyghton,M.P., Roel,G., Eichhorn,P.J., Hijmans,E.M., Maurer,I.,
            Destree,O. and Bernards,R.
  TITLE     PR72, a novel regulator of Wnt signaling required for Naked cuticle
            function
  JOURNAL   Genes Dev 19 (3), 376-386 (2005)
   PUBMED   15687260
REFERENCE   8  (bases 1 to 2182)
  AUTHORS   Li,C., Franklin,J.L., Graves-Deal,R., Jerome,W.G., Cao,Z. and
            Coffey,R.J.
  TITLE     Myristoylated Naked2 escorts transforming growth factor alpha to
            the basolateral plasma membrane of polarized epithelial cells
  JOURNAL   Proc Natl Acad Sci U S A 101 (15), 5571-5576 (2004)
   PUBMED   15064403
REFERENCE   9  (bases 1 to 2182)
  AUTHORS   Katoh,M.
  TITLE     Molecular cloning, gene structure, and expression analyses of NKD1
            and NKD2
  JOURNAL   Int J Oncol 19 (5), 963-969 (2001)
   PUBMED   11604995
REFERENCE   10 (bases 1 to 2182)
  AUTHORS   Wharton,K.A. Jr., Zimmermann,G., Rousset,R. and Scott,M.P.
  TITLE     Vertebrate proteins related to Drosophila Naked Cuticle bind
            Dishevelled and antagonize Wnt signaling
  JOURNAL   Dev Biol 234 (1), 93-106 (2001)
   PUBMED   11356022
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC116351.2.
            
            On May 17, 2019 this sequence version replaced NM_033120.3.
            
            Summary: This gene encodes a member of a family of proteins that
            function as negative regulators of Wnt receptor signaling through
            interaction with Dishevelled family members. The encoded protein
            participates in the delivery of transforming growth factor
            alpha-containing vesicles to the cell membrane. Alternatively
            spliced transcript variants encoding multiple isoforms have been
            observed for this gene. [provided by RefSeq, Aug 2012].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB062887.1, AF358137.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968968
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000296849.10/ ENSP00000296849.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-281               AC116351.2         34730-35010
            282-317             AC116351.2         35107-35142
            318-397             AC116351.2         35409-35488
            398-458             AC116351.2         58080-58140
            459-586             AC116351.2         59300-59427
            587-682             AC116351.2         60163-60258
            683-830             AC116351.2         60684-60831
            831-915             AC116351.2         61317-61401
            916-1043            AC116351.2         62185-62312
            1044-2182           AC116351.2         63733-64871
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="5"
                     /map="5p15.33"
     gene            1..2182
                     /gene="NKD2"
                     /gene_synonym="Naked2"
                     /note="NKD inhibitor of WNT signaling pathway 2"
                     /db_xref="GeneID:85409"
                     /db_xref="HGNC:HGNC:17046"
                     /db_xref="MIM:607852"
     exon            1..281
                     /gene="NKD2"
                     /gene_synonym="Naked2"
                     /inference="alignment:Splign:2.1.0"
     CDS             257..1612
                     /gene="NKD2"
                     /gene_synonym="Naked2"
                     /note="isoform 1 is encoded by transcript variant 1;
                     protein naked cuticle homolog 2; Dvl-binding protein NKD2;
                     NKD2, WNT signaling pathway inhibitor"
                     /codon_start=1
                     /product="protein naked cuticle homolog 2 isoform 1"
                     /protein_id="NP_149111.1"
                     /db_xref="CCDS:CCDS3859.1"
                     /db_xref="GeneID:85409"
                     /db_xref="HGNC:HGNC:17046"
                     /db_xref="MIM:607852"
                     /translation="MGKLQSKHAAAARKRRESPEGDSFVASAYASGRKGAEEAERRAR
                     DKQELPNGDPKEGPFREDQCPLQVALPAEKAEGREHPGQLLSADDGERAANREGPRGP
                     GGQRLNIDALQCDVSVEEDDRQEWTFTLYDFDNCGKVTREDMSSLMHTIYEVVDASVN
                     HSSGSSKTLRVKLTVSPEPSSKRKEGPPAGQDREPTRCRMEGELAEEPRVADRRLSAH
                     VRRPSTDPQPCSERGPYCVDENTERRNHYLDLAGIENYTSRFGPGSPPVQAKQEPQGR
                     ASHLQARSRSQEPDTHAVHHRRSQVLVEHVVPASEPAARALDTQPRPKGPEKQFLKSP
                     KGSGKPPGVPASSKSGKAFSYYLPAVLPPQAPQDGHHLPQPPPPPYGHKRYRQKGREG
                     HSPLKAPHAQPATVEHEVVRDLPPTPAGEGYAVPVIQRHEHHHHHEHHHHHHHHHFHP
                     S"
     exon            282..317
                     /gene="NKD2"
                     /gene_synonym="Naked2"
                     /inference="alignment:Splign:2.1.0"
     exon            318..397
                     /gene="NKD2"
                     /gene_synonym="Naked2"
                     /inference="alignment:Splign:2.1.0"
     exon            398..458
                     /gene="NKD2"
                     /gene_synonym="Naked2"
                     /inference="alignment:Splign:2.1.0"
     exon            459..586
                     /gene="NKD2"
                     /gene_synonym="Naked2"
                     /inference="alignment:Splign:2.1.0"
     exon            587..682
                     /gene="NKD2"
                     /gene_synonym="Naked2"
                     /inference="alignment:Splign:2.1.0"
     exon            683..830
                     /gene="NKD2"
                     /gene_synonym="Naked2"
                     /inference="alignment:Splign:2.1.0"
     exon            831..915
                     /gene="NKD2"
                     /gene_synonym="Naked2"
                     /inference="alignment:Splign:2.1.0"
     exon            916..1043
                     /gene="NKD2"
                     /gene_synonym="Naked2"
                     /inference="alignment:Splign:2.1.0"
     exon            1044..2182
                     /gene="NKD2"
                     /gene_synonym="Naked2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1897..1902
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NKD2"
                     /gene_synonym="Naked2"
                     /note="hexamer: CATAAA"
     polyA_site      1921
                     /gene="NKD2"
                     /gene_synonym="Naked2"
                     /note="major polyA site"
ORIGIN      
        1 agagggagcc gggccgccgt cgctgccgcc gctgtccccg cgccctgcgc ccggtggccc
       61 cccacctccg ccccgcggcc gtacctggcg ccccctggct cccccggccc ccgcgcggcg
      121 tggagccatc ttccctcacc tcctaccggc accctagctt gctcccggcc catgcggccc
      181 ccgcgggctc ccggccccgg cttcagaact cagccctgca cctgagcgcg gggcccggcg
      241 gggcgtggcg gcggcgatgg ggaaactgca gtcgaagcac gccgccgccg cccgcaagcg
      301 gagagagagc ccggaagggg acagcttcgt ggcgtccgcg tacgcgagcg gccgcaaagg
      361 cgcggaggaa gcggagcggc gcgcgcggga caagcaggag ctgcccaatg gggaccccaa
      421 ggaggggcct ttccgggagg accagtgtcc cctacaggtg gcactccccg ctgagaaagc
      481 tgagggccgc gagcacccgg gacaactcct cagcgcagat gacggagaga gggcagcaaa
      541 ccgcgagggc ccgcgaggac cgggcgggca gcgcctcaac attgacgcac tccagtgcga
      601 tgtctcggtg gaggaggacg accgccagga gtggacgttc acgctctatg actttgacaa
      661 ctgcgggaag gtcaccaggg aggacatgtc cagcctcatg cacaccatct atgaggtcgt
      721 ggatgcctcg gtcaaccact cctcgggcag cagcaagacc ctccgtgtga agctaaccgt
      781 cagccctgag ccctccagca agaggaagga gggtcctcct gctggccagg accgggagcc
      841 cacccgttgc aggatggagg gtgaactggc agaggagcca agggtggctg acaggaggtt
      901 gtctgcacac gtcaggaggc ccagtactga cccccagccc tgctcggagc gggggcccta
      961 ctgcgtggac gagaacacgg agcgcagaaa ccactacctg gacctcgccg ggattgagaa
     1021 ctacacgtcc agattcggcc ctgggtcccc tcctgtgcaa gcaaagcagg agccccaggg
     1081 cagggcctcg cacctccagg cccggtcccg ctcccaggag ccagatacac atgccgtaca
     1141 ccaccgcagg tcacaggtgc tggtggaaca cgtcgtgcca gcctcggagc ctgctgcccg
     1201 ggccctggac acgcagcccc ggccgaaggg gccggagaag cagttcctca agtcccccaa
     1261 gggctccggg aagccgcctg gggtgccagc cagcagcaag tccgggaaag ccttcagcta
     1321 ctacctgccg gccgtcctgc cgccccaggc ccctcaggac ggccaccacc tcccgcagcc
     1381 cccaccgcca ccctacggcc acaagcggta ccgccaaaag ggcagggagg gccactcgcc
     1441 actcaaggcc ccacacgctc agcctgccac agtggagcac gaggtggtgc gggacctgcc
     1501 gcccacgcca gcaggagagg gctacgcggt gccagtgatc cagcggcacg agcaccacca
     1561 ccaccacgag caccaccacc accaccacca ccaccacttc cacccgtcct agcgccactg
     1621 ccaagcacac ctcgctccca gcacaccaca gcccgcgacc tcagggcagg gagcagagca
     1681 gctgccggct gtgtgcccat ggggagccca gcccccaccc cccacctccg acagcaaaca
     1741 gcaactgact gcaggtgctg gcatgatgga ggtggtgcac cttggacacg tggacaaggc
     1801 ccaggcgccc tctgctcttc tgccctcgat gccacatggc ggtgaacaca tctgaagcca
     1861 ctatgtttcc tggctctaag gctcgtctgt gtaacccata aaacctgctt tgattccaaa
     1921 atgaggccct ggagtgcgca aggagtgccc ggatgcttgg ggtgggtgtt cctcccgggg
     1981 cttcaagggg tgactgctgt aggctgtccc agtgcgaggc cgggagcgaa tggaaaagct
     2041 gaggctttgc ctggcttgtg catcatgaag tgagagggga cagggccggt ggggggaagc
     2101 agcttgtgct tagcagggag catccgcggc tccccgcagg aggccaagca gcagaaggca
     2161 gcagtgctag aaagagaagt gc
//
