LOCUS       NM_033119              17039 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens NKD inhibitor of WNT signaling pathway 1 (NKD1), mRNA.
ACCESSION   NM_033119
VERSION     NM_033119.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 17039)
  AUTHORS   Dai,Y., Liu,J., Li,X., Deng,J., Zeng,C., Lu,W., Hou,Y., Sheng,Y.,
            Wu,H. and Liu,Q.
  TITLE     Let-7b-5p inhibits colon cancer progression by prohibiting APC
            ubiquitination degradation and the Wnt pathway by targeting NKD1
  JOURNAL   Cancer Sci 114 (5), 1882-1897 (2023)
   PUBMED   36445120
  REMARK    GeneRIF: Let-7b-5p inhibits colon cancer progression by prohibiting
            APC ubiquitination degradation and the Wnt pathway by targeting
            NKD1.
            Erratum:[Cancer Sci. 2023 Aug;114(8):3466-3469. doi:
            10.1111/cas.15904.. PMID: 37401168]
REFERENCE   2  (bases 1 to 17039)
  AUTHORS   Liu,Q., Dai,Y., Yu,H., Shen,Y., Deng,J., Lu,W. and Jin,J.
  TITLE     [NKD1 promotes glucose uptake in colon cancer cells by activating
            YWHAE transcription]
  JOURNAL   Nan Fang Yi Ke Da Xue Xue Bao 43 (4), 585-589 (2023)
   PUBMED   37202194
  REMARK    GeneRIF: [NKD1 promotes glucose uptake in colon cancer cells by
            activating YWHAE transcription].
REFERENCE   3  (bases 1 to 17039)
  AUTHORS   Li,L., Gao,R., Huangfu,W., Zhang,F. and Wang,R.
  TITLE     Clinical Significance of NKD Inhibitor of WNT Signaling Pathway 1
            (NKD1) in Glioblastoma
  JOURNAL   Genet Res (Camb) 2023, 1184101 (2023)
   PUBMED   36969985
  REMARK    GeneRIF: Clinical Significance of NKD Inhibitor of WNT Signaling
            Pathway 1 (NKD1) in Glioblastoma.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 17039)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 17039)
  AUTHORS   Song,X., Wu,R., Tao,Q., Zhao,L. and Ji,Z.
  TITLE     miR-532 promotes colorectal cancer invasion and metastasis by
            targeting NKD1
  JOURNAL   Cell Mol Biol (Noisy-le-grand) 65 (6), 52-55 (2019)
   PUBMED   31472047
  REMARK    GeneRIF: miR-532 is highly expressed in colorectal cancer cells and
            directly inhibits NKD1 expression
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 17039)
  AUTHORS   Creyghton,M.P., Roel,G., Eichhorn,P.J., Hijmans,E.M., Maurer,I.,
            Destree,O. and Bernards,R.
  TITLE     PR72, a novel regulator of Wnt signaling required for Naked cuticle
            function
  JOURNAL   Genes Dev 19 (3), 376-386 (2005)
   PUBMED   15687260
REFERENCE   7  (bases 1 to 17039)
  AUTHORS   Yan,D., Wiesmann,M., Rohan,M., Chan,V., Jefferson,A.B., Guo,L.,
            Sakamoto,D., Caothien,R.H., Fuller,J.H., Reinhard,C., Garcia,P.D.,
            Randazzo,F.M., Escobedo,J., Fantl,W.J. and Williams,L.T.
  TITLE     Elevated expression of axin2 and hnkd mRNA provides evidence that
            Wnt/beta -catenin signaling is activated in human colon tumors
  JOURNAL   Proc Natl Acad Sci U S A 98 (26), 14973-14978 (2001)
   PUBMED   11752446
REFERENCE   8  (bases 1 to 17039)
  AUTHORS   Katoh,M.
  TITLE     Molecular cloning, gene structure, and expression analyses of NKD1
            and NKD2
  JOURNAL   Int J Oncol 19 (5), 963-969 (2001)
   PUBMED   11604995
REFERENCE   9  (bases 1 to 17039)
  AUTHORS   Wharton,K.A. Jr., Zimmermann,G., Rousset,R. and Scott,M.P.
  TITLE     Vertebrate proteins related to Drosophila Naked Cuticle bind
            Dishevelled and antagonize Wnt signaling
  JOURNAL   Dev Biol 234 (1), 93-106 (2001)
   PUBMED   11356022
REFERENCE   10 (bases 1 to 17039)
  AUTHORS   Yan,D., Wallingford,J.B., Sun,T.Q., Nelson,A.M., Sakanaka,C.,
            Reinhard,C., Harland,R.M., Fantl,W.J. and Williams,L.T.
  TITLE     Cell autonomous regulation of multiple Dishevelled-dependent
            pathways by mammalian Nkd
  JOURNAL   Proc Natl Acad Sci U S A 98 (7), 3802-3807 (2001)
   PUBMED   11274398
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            BC051288.1 and AC007608.7.
            
            On Nov 22, 2018 this sequence version replaced NM_033119.4.
            
            Summary: In the mouse, Nkd is a Dishevelled (see DVL1; MIM
            601365)-binding protein that functions as a negative regulator of
            the Wnt (see WNT1; MIM 164820)-beta-catenin (see MIM 116806)-Tcf
            (see MIM 602272) signaling pathway.[supplied by OMIM, Jun 2003].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY061883.1, BC051288.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA1968540
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000268459.6/ ENSP00000268459.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2304              BC051288.1         67-2370
            2305-17039          AC007608.7         62466-77200         c
FEATURES             Location/Qualifiers
     source          1..17039
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="16"
                     /map="16q12.1"
     gene            1..17039
                     /gene="NKD1"
                     /gene_synonym="Naked1"
                     /note="NKD inhibitor of WNT signaling pathway 1"
                     /db_xref="GeneID:85407"
                     /db_xref="HGNC:HGNC:17045"
                     /db_xref="MIM:607851"
     exon            1..183
                     /gene="NKD1"
                     /gene_synonym="Naked1"
                     /inference="alignment:Splign:2.1.0"
     CDS             159..1571
                     /gene="NKD1"
                     /gene_synonym="Naked1"
                     /note="naked cuticle-1; Dvl-binding protein; naked-1;
                     naked cuticle homolog 1; NKD1, WNT signaling pathway
                     inhibitor"
                     /codon_start=1
                     /product="protein naked cuticle homolog 1"
                     /protein_id="NP_149110.1"
                     /db_xref="CCDS:CCDS10743.1"
                     /db_xref="GeneID:85407"
                     /db_xref="HGNC:HGNC:17045"
                     /db_xref="MIM:607851"
                     /translation="MGKLHSKPAAVCKRRESPEGDSFAVSAAWARKGIEEWIGRQRCP
                     GGVSGPRQLRLAGTIGRSTRELVGDVLRDTLSEEEEDDFRLEVALPPEKTDGLGSGDE
                     KKMERVSEPCPGSKKQLKFEELQCDVSMEEDSRQEWTFTLYDFDNNGKVTREDITSLL
                     HTIYEVVDSSVNHSPTSSKMLRVKLTVAPDGSQSKRSVLVNQADLQSARPRAETKPTE
                     DLRSWEKKQRAPLRFQGDSRLEQSGCYHHCVDENIERRNHYLDLAGIENYTSQFGPGS
                     PSVAQKSELPPRTSNPTRSRSHEPEAIHIPHRKPQGVDPASFHFLDTPIAKVSELQQR
                     LRGTQDGSKHFVRSPKAQGKSVGVGHVARGARNKPPLGPAIPAVSPSAHLAASPALLP
                     SLAPLGHKKHKHRAKESQQGCRGLQAPLASGGPVLGREHLRELPALVVYESQAGQPVQ
                     RHEHHHHHEHHHHYHHFYQT"
     exon            184..216
                     /gene="NKD1"
                     /gene_synonym="Naked1"
                     /inference="alignment:Splign:2.1.0"
     exon            217..350
                     /gene="NKD1"
                     /gene_synonym="Naked1"
                     /inference="alignment:Splign:2.1.0"
     exon            351..417
                     /gene="NKD1"
                     /gene_synonym="Naked1"
                     /inference="alignment:Splign:2.1.0"
     exon            418..524
                     /gene="NKD1"
                     /gene_synonym="Naked1"
                     /inference="alignment:Splign:2.1.0"
     exon            525..620
                     /gene="NKD1"
                     /gene_synonym="Naked1"
                     /inference="alignment:Splign:2.1.0"
     exon            621..768
                     /gene="NKD1"
                     /gene_synonym="Naked1"
                     /inference="alignment:Splign:2.1.0"
     exon            769..853
                     /gene="NKD1"
                     /gene_synonym="Naked1"
                     /inference="alignment:Splign:2.1.0"
     exon            854..981
                     /gene="NKD1"
                     /gene_synonym="Naked1"
                     /inference="alignment:Splign:2.1.0"
     exon            982..17039
                     /gene="NKD1"
                     /gene_synonym="Naked1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4499..4504
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NKD1"
                     /gene_synonym="Naked1"
                     /note="hexamer: AATAAA"
     polyA_site      4524
                     /gene="NKD1"
                     /gene_synonym="Naked1"
                     /note="major polyA site"
     regulatory      8509..8514
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NKD1"
                     /gene_synonym="Naked1"
                     /note="hexamer: AATAAA"
     polyA_site      8529
                     /gene="NKD1"
                     /gene_synonym="Naked1"
     regulatory      16457..16462
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NKD1"
                     /gene_synonym="Naked1"
                     /note="hexamer: AATAAA"
     polyA_site      16478
                     /gene="NKD1"
                     /gene_synonym="Naked1"
     regulatory      17019..17024
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NKD1"
                     /gene_synonym="Naked1"
                     /note="hexamer: AATAAA"
     polyA_site      17039
                     /gene="NKD1"
                     /gene_synonym="Naked1"
ORIGIN      
        1 agtcgggccg cggcgacggc ggcaggagcg cgtcccggcg ccgcctcggg ctccgctcgg
       61 ctcgggggct gcttcgggag gaggagagcc aagggaggcg ccaggcccgc gggccgggcg
      121 catggcttag ggacgctccc ggccgccgca gccccagcat ggggaaactt cactccaagc
      181 cggccgccgt gtgcaagcgc agggagagcc cggaaggtga cagcttcgcc gtgagcgctg
      241 cctgggctcg gaagggcatc gaggagtgga tcgggagaca gcgctgcccg ggcggtgtct
      301 cgggaccccg acagctgcgg ttggcgggca ccataggccg aagcacccgg gagctcgtgg
      361 gcgacgtgtt gagagacacg ctcagcgagg aagaggagga cgactttcgg ctggaagtgg
      421 ccctgcctcc tgagaagact gacgggctgg gcagcggaga tgagaagaag atggagagag
      481 tgagcgaacc ctgcccaggc tccaagaagc agctgaagtt tgaagagctc cagtgcgacg
      541 tgtccatgga ggaggacagc cggcaggagt ggaccttcac cctgtatgac tttgacaaca
      601 acggcaaggt cacccgagag gacatcacca gcttgctgca caccatctat gaggtggtgg
      661 actcctctgt caaccactcc ccaacatcca gcaagatgct gcgggtaaag ctcaccgtgg
      721 cccccgatgg cagccagagc aagaggagcg tccttgtcaa tcaggctgac ctgcagagcg
      781 caaggccccg agcagagacc aagcccactg aggacctgcg gagctgggag aagaagcagc
      841 gagccccgct caggttccag ggtgacagcc gcctggagca gtctggctgc taccaccatt
      901 gcgtagatga gaacatcgag aggagaaacc actacttaga tctcgccggg atagaaaact
      961 acacgtccca atttgggcct ggctcccctt ccgtggccca gaagtcagaa ctgccccccc
     1021 gcacctccaa tcccactcga tctcgctccc atgagccgga agccatccac atcccacacc
     1081 gaaagcccca aggcgtggac ccggcctcct tccacttcct tgacacccca atcgccaagg
     1141 tctcagagct ccagcaacgg ctccggggca cccaggacgg gagcaagcac tttgtgaggt
     1201 cccccaaggc ccagggcaag agtgtgggtg tgggccacgt ggccagaggg gcaagaaaca
     1261 agccccctct gggacccgcc atccctgcgg tgtccccctc cgcccacctg gctgccagcc
     1321 cggccctcct cccctcccta gcccccctcg ggcacaagaa gcacaagcac cgagccaagg
     1381 agagccagca gggctgccgg ggcctgcagg caccactggc ctcaggtggc cctgtcctgg
     1441 ggcgggagca cctgcgggag ctgcccgcct tggtggtgta tgagagccag gccgggcagc
     1501 cggtccagag acatgagcac caccaccacc atgaacatca ccaccattac caccacttct
     1561 accagacata gagcccctcc ccagggcccc accctgccat atgaaggacc ccacccccga
     1621 caccacaagg cattattatt ctattaatta ttgttattat gatgattatt gttattaata
     1681 attattgtta ctccactaat atttagctag cctacatgta gaagatctat ggaaacacag
     1741 aactaaactt ttatttatat gttgtgggga ctgcataact agcccaggaa atgactctgg
     1801 ttttgagtgg cctgtaatgg gcagaggctc cctcggggct cgggatctgc agcatctatg
     1861 tggatcagcc cacacccttc ccagagccag ggagccctta gcctcaactc tgccccaccc
     1921 cagcctcttc caggagagca cccttacttg gcccggcttc cagagaccct cgaaatctcc
     1981 gagaagataa acagctgcta cctcttagta ttattctgat tttattttgc ccttaactga
     2041 ttgttatatg ctacaaggga tttcagtgga ctgcagagtg cgaacgtctt gctgttgtgt
     2101 ttttatgtcc caacctagaa accttagctg tcttttctgg agttgtcttt catgcttttc
     2161 cagtgggatc aagccctttt ccccaagagt cccatctctt ctgccatgca cgacatgaaa
     2221 atccacttct cttccttctc cttcttctct ctctacttta cagtgataca cacacgtatt
     2281 taaggactat ccctgagacc atccttctca ttttggaaac tgctagggag ggaaccaacc
     2341 acttaaacaa gcgtggtttt ccaagatcca ggcaattgtg tgctgttggt tgtggtggag
     2401 gatgtggcac atatatacgt acctacacgg ttctctaaca gagctttctg tatctataga
     2461 tagatgccat ctgtccattt ctatgtatct ttctataaat atacactctc aggtatcttt
     2521 tctggtgtgt ataaatcagt ggtttgcttc cctggatgct tctggaaccc agatgtgacc
     2581 ctgcttgtct ccttttgggg ctggacggct ctcagatgct ttcatcagag gtccattctc
     2641 gggttttggg gtctgatgga ttttggagag aactgctggg gtacagaaac ttggtgggaa
     2701 aaggggactg tggccagagt tgggaccctg gagcagcatc ctctgcagag aaggattttg
     2761 tctggccaga gcctggagaa acctgaaaaa gaaccagtca gctagccagg gtctcagaga
     2821 aaagcagatt acacactcaa attgggtaat ttgagcagag cttaataaag gcagtattta
     2881 caaagtgtgg gctaagcctc ccatgagagt gcagaaccct ggggctagca gtgtggggcg
     2941 ctattcccac ccctgggcct gagtggggaa ggagttatct ttagcagaca gaggattgct
     3001 ggaggagtga gaggggcacc tgaaggagct gtgacctttg cttaaaggat gcagccagtc
     3061 atggggaccc tccagggaaa ttaatatcct gacctgcttt cctgcccccc agccccctca
     3121 atccattggc tgaggccgct ggaagccacc gggccaaggg agcttgttga tgtgggtcac
     3181 acgggcatgt tcccaggtca gagaggagag tggagagtga atctagggag actcaagagg
     3241 gagaagtgac tccagctacc ttcctttctg ccgttttgtc tcccagctgg cattctcttt
     3301 tccagagcct cgagtttggg tttaatgtag ttagtaagga gttacttcca aaggtagtgg
     3361 gcttagttgt caccgtattc ttaattttat taatgactga tgtgcctggg attggattaa
     3421 tacattaatc cttaggacag ccccatgagg cagcttggtg gcagctcagg aagcatgtct
     3481 aaggctcaga agttcataac tcccttgagg ccgcacagag ggcggagctg gaattctggt
     3541 ctcccactcg atcccttcgc cactggtgca gagcttaatc acggccatgg gctgcccttg
     3601 ttttgggttg tgggtttctg ttcagtgaga acgcaactca atccaaagtg aatgaaacca
     3661 agttggaagg cagggaggga actggctttg agatgacagg gtccaggatg tgaatgcagc
     3721 tgagactggt tcttgtccct ccctcttgtc cctcggattg ataacttgta ctcaactaca
     3781 tgcttttgtc agggaaccct ggctgctggc cttctgggtc ccctaaccat aagaaaaagg
     3841 accttccgtt agtgtaaagc ccaaggcagg attctgcttg gctgggcttc gatctggtac
     3901 ccatagctgg accaatcatg acatcccaga tggggtcacg tggccaacct gccgcaaggg
     3961 ggtggggtct gtaccggaag acaggagggg aggggtgcag atcggacagg aagtaatggc
     4021 atcccaggcc ccgaattgct gcaaccctgg aggccagccc ggagttgaga ctactggttt
     4081 tagagcagtt cctctgcctc cctaagctcc acacctgtca ggattctgtt acttcttggt
     4141 aactgggact tgcccaactt taaaaacatt atttaaaaaa atagtaatgt gcacatgtaa
     4201 aagattcaaa tagtatatat acaaggtgta cagtaaaaag taaacttccc tccatcccag
     4261 gcctgccagc atccctgatg ccgactttct gggtgtggcc tagggcccct cagtgtaatg
     4321 taggggttgt gagcacagac tttggtgcca gtttgctagg ttcgaatcct gactccctct
     4381 ttgtagctct gtgcttcaat tgaaatactg tgcctcagtt tctcctttat aaaggcaggg
     4441 atcatgagag tgcctgtccc ttgtgagcac tatgaaagtg ttagctgttc tttaccagaa
     4501 taaatgcatt tctatatctt cccatatgca ttttgttaat ttttaaagta tttcaaacac
     4561 aaagtttgaa acagaaaatt gtgtaacatt aactatgaac ttaccaccca gaatttacaa
     4621 atgctgacat tttgcaatat ttatttcgga tctattttta agggggggaa ccctgcagtt
     4681 actgcttaat cctcttccca ccccaccctt ttatttttac acaaggagca tagtgttcat
     4741 acttatgcta tttttttcag taactgaata tatttggaga tctccctccc taggtcatag
     4801 agctctgccc ccttctctct aacagctgct cagccttctg tgactagagg tgctgtcacc
     4861 agcttgcccc atcccctcct ggtggtcata agggtcatct ctcatcccag gctctggcga
     4921 acactgtgct gagagtcctt gcatacagct ctcccggtgc ccacaggcag gaacacaggc
     4981 gacagaaagt ttccacgtgg aactgcagag ttcaagagca agtgtgattt caattttgat
     5041 gagattgtac atgacctcta aaagaggtta tactggccgg gcacggtggc tcacgcctgt
     5101 aatctcaaca ctttgggaga ccgaggtggg tggatcacct gaggtcagga gttcgagacc
     5161 aacctgggca acatggtgaa accccgtttc tactaaaaat acaaaaaatt agctgggcat
     5221 ggtggtgcgt gcctgtaatc tcagctactc gggaggccaa ggcacgagaa tcgcttgaac
     5281 ctgggaggca gagattgcag tgagctaaga ttgcaccact gtactccagc ctgggcgaca
     5341 gagtgagact ccatctcaaa taaataaaga ggttatactg aataatgagg aatcaaagga
     5401 agaagaagaa agagagagga aggaaggttg gaaggaagga aggagggaaa attagaaggg
     5461 gaaaccatga ttgctggtga ggttttgagc acattttcct gcaggctggt atgggtgaga
     5521 ggtttggtct tgtttgcaaa tcttctgaag gccattccag aggagcagtt gccactgccc
     5581 catcccctga gctctgagcg tgggggttcc cctggggaga ctcctggtga gaggatgccg
     5641 atttctgctg atctgtcact gggtaccgag gactgggtgt gtttaaggca gacagccagg
     5701 tgaggatccc agctactggg gcctgctgtc atctcctggg agtacccggg ggtcaggagc
     5761 ctaggggact cttgcacttc acatccagcc atgctaatta cactttttgg caaaggaaac
     5821 agctaggagc agtttctttc actcctacag ccccgttttc tcagtgttta gacctcgaat
     5881 tattactggg ctagagggaa ggcagcctct gaagtgtggc aggaggaggg gaagtctgcc
     5941 tgcatcttgg tgtgtctgtc agatgccagc actaataacc tggcttctgt gaggcctgtc
     6001 agtgctctca ggaatgaaag gggacccctg agaggtgctc agtaccagca ggctgtgaat
     6061 gctctctacc caccaccctc acctcctcgt taaagatggt gctacctgcc acacagcaga
     6121 catctggtcg ctgcacaccc gaaagacccc aaggcagtct gccccttgtc cagccacacg
     6181 ccagcaccca ccctcctggc ccctgcctcg gcctccccag accagctgca cccagccccc
     6241 aacacgcacc ccttctccag atgtgtgcag ggcctcattt tgcagagcaa agacagatgt
     6301 ttcagccaca cgctttatta acttctaaaa cctgtgctca ggacactctt caacagtcat
     6361 gaaaagtttg atcacttgcc acagtcagga cctttgtgtg gggctctgat ctgatgttcg
     6421 gtctcatcat ctcccaaacc agcagtcgtt tgtaccccaa ccctctgctc aggggctcat
     6481 acccccaaat gattttcctg atttatgtat ttccctacaa agggctttct atacctagca
     6541 tctgcctcca gcatgagaag ggggaatagg tgagacccat ttgccagtag cagacgggga
     6601 ccctggggag aaaatggcag agcctgttgg agactccctg tctccagctg accagccaat
     6661 gggattcctc ttccctccac tgtctcccac aaagtagaag aatcctggta catttagccc
     6721 atgagcctgg cacagatccc tatctagaca tgaggccctt tagacatgac tttggcattg
     6781 accagcctgt tggcaatggg tcggggaggc agaggggatg ctcacaccag taattctcat
     6841 cccctgaatg cttgggatca cctggggaga gttcacaaaa tactggtgca ggggtcccac
     6901 ctctgatgat gctgagtggt gggtctgggg tgtggcccag gcatcatgat gtttcaggcc
     6961 cccaggtgac ttcttaggca gcccagctaa gcccctagag ccttgcaatt tcccccaaat
     7021 gacctcagag ggcccgattt gagggaaatg cctaacttca ggggccgtaa gaatccccca
     7081 gggagcatgt gaaatgcaga taccaggccc acccccagag atgagctgag gtgggtcagg
     7141 ggtgaagtgc agggatcagt gtttttcaca agctccatac ctccaggaaa tggtgttgtg
     7201 gttgggcccg tagaaaacat tctgagagtc ctgttgcctg tgccttggtg cacgtggggt
     7261 ggaatcccag tggccctgcc ttgaggagga tgtgcattaa cgtggtaggg gagacagaga
     7321 cagctccacc tgccccctgc cccaccgggg acctccaaaa acttcatgga tgttagagca
     7381 agcagccatg ctgcagcaga ggatgaggct ggcggattta gtaagagccc tctgtgtttg
     7441 ggctgagttc tttctctagt tgccctgtca tctggcctct ggataaccca cctctcctcc
     7501 ctcatcctaa aattacagat ggcgaaagat ggccacattt agtgagaccc ctaaggtcct
     7561 ccaactaggg tgggtccaca gtggcccctg gtgcatggac cacacactct cttccctcct
     7621 ctggctcagg actacggtct gaaattaggg agatatgaat gtctttcttg aaaacttctc
     7681 ttcccagtct tcccactttg cttgggggtc cttggtcaag gccagctttg gggactaggg
     7741 cttgttgcga ctaccagctg tctcattttg ctgtactgca aactcaggct tggttccaag
     7801 cttatggggg ccctgtcctt cccctagtag ggtttgtttt ggggtcacat ctggtcatac
     7861 ccttcagaga gctcttcccc agcctctaca tcagggagag aggtaggtag ggaggagcat
     7921 tcaaggatta gaagaaggac taaagtacaa cagccttgga ggaactgcca ggaactaagg
     7981 gcgagcactg gagaaggcaa cctgggaccc cctgcgcttc tgagcaggaa gaccaagacc
     8041 ttcaggggcc ctaagcactg aaaacatcat tcctcatccc caagccctgg catccccctg
     8101 ttcttctaaa ataattcttt tctaggtatt tctgattgca aaattctgga tgggttcatc
     8161 caagctgacc tttgctgttt tttcccttcc caacaaggcc tcactttttg gagccacctt
     8221 agctggtgcc taggcagagg ggcagtcagc agtggttatc aggatcctgg ctctatgggt
     8281 tgccttcctc ctggtctgta aagcccctgc aggcagggac ttcttagata gctgcttcct
     8341 tagggcatgg catgtagtgg gtggttaatg aatggaagag agggaatgag tgatcaaggg
     8401 agggaggagg gagtggagtg gagatttctc atcctttcct gttaatttat gacatcctcc
     8461 tgcctatgag tccttgactc tggagtttta caaagcagtc acatttcaaa taaaagtctg
     8521 ggaaagcaac acatcatcgc caacttttaa ttttgctaaa taaggatatt agaaaaagaa
     8581 tagaaaattg cagtccctta ctgtttaaag aaaaaccaaa agaagttaaa aagaaaaaaa
     8641 aaacaacctc attatgttgt ctttgttttt gttttgtttt ctttccttgg aaatcagtga
     8701 aagtttggtc agccacacat tgtagtaggg ctagaatttt tcttcccatt ttagagacgg
     8761 gagacctgag actccaggtg ggggtgtgga gtccaaatgg acagttcttc ccaaagctga
     8821 gcgcagaccg gcttgcaggt cctttttcca gtcatggcct cagctgtctg caaaggactt
     8881 gggggaggct gacagctctg atgtctggag agggggcttc tgcaacctcc aacactggtc
     8941 agtaactcca cagaggccat tggcgggggt ggtctgcttg tatcagcagg cctgtcatgg
     9001 ccagttcaat ggtcggggct gaactatgtc tcttggtgcc tggtagaggg gttggagtgg
     9061 gcctgaggcc aacttgctca gagccacact ccccaggaag ctgtggagtc tggaactctg
     9121 ccctgccttc ctgtcccctg cccactctaa gcctccagaa gtcaatcctc tgctcaccgg
     9181 gaccgtacct agcctggccc atcgatgtgt catgtttgac ccatcaaggg tttcaaaaat
     9241 ttttaattaa tttccaaaat ctgagggttt cacctaaaaa cctggatgtt tggcttttct
     9301 cataaaaatc agaaagtggg tttaccccag gctcctgttc ccacatggct gcaatccgcc
     9361 cgcctcttcc aatggtgcac atgccttttt tcctgcgccc ggagcccctc tcagcctgat
     9421 tttgaggcct ggctttgatg cgattcagct catgacccct gcgcagcctc aagcacctaa
     9481 gttttggagg tcagtgatgc cacttaaaaa cctgttcagg gcagagcgtt agacaggagg
     9541 atggctaagg gagcagcttc aggcatggga ggactttcat ggggaagtct ttggggcagt
     9601 cctggagacc ctcacaatgt ggccccactc ctcctttaga tggggcaagg tgggctggaa
     9661 cccccagcaa agggtcccag gctgtgtgag accccgccca gcactcctta aacagccaag
     9721 acaggtggaa agccccaact caaaagactg gctggattgt acccggctca gtgacttccc
     9781 ctctttgaga ctcagtttgc ttttctgtaa agtggaccta atgaggcccg cttggcttca
     9841 gctccaggga ctttggagat ttaagcaaag caagaaatgg gaaaggagtt tgttacatat
     9901 caggcttcct ggaggaatgc cccccagctc tcttggaggg ggaagcaaaa agactgctga
     9961 tcaggcaggg gacgggaact ccccgagtgg ccacatgggc agtctcagta ggagtctcag
    10021 gccaggctct gtctgtggat tcccacttct ctgggctgca tctcactgtt caggctagag
    10081 tctggtcctt gcccatctgc aggtccagga agatgtggcc ccaaattctc tggacctgat
    10141 gtcctagatc ctgtgcactt ggcttcatgg atagagtctg agaaggttgc aggatccagt
    10201 agctggacca cagaatgcag cctgggggtt tggctaagat gagcagactg gcccatggtt
    10261 gagactttgg acctgagctg agtcctggtt ctactacttc ctagatgtgt gacctgggac
    10321 aagtgactca cctctgtgcc ttggtgtttt tctctaacat ggggctaatt ggggtaacta
    10381 ccttactgag ctgttatgag taaatgatcc agtgggcgtg aagcgttcag cgcagagcct
    10441 ggcacgtaat aggccctcga taagtaaaac agcaacagta gctgcagtta ggagaatgtg
    10501 accccagaag gctcatggga aaggaggtgc agtcctgatg gatgaagggt acctgtcctc
    10561 caccccggct gcagagcccc cagtgagaca gctgctgtgg catgctgcct ctgctacaca
    10621 ctcaaaaaac acttgaggac atgacttttc cctctcaagt ttgatgggat cactctgttc
    10681 cttccagacc cgacttgtgg tgtgaacttg gacaagcccc tgctgtctct ggccttcagt
    10741 accatatttg tattatgaaa ctgcccttgg cttgaagcta gactatgggg gtggcagctc
    10801 taagtcctac aaacagtagg ggtagtggtg gggatgggca gcctgaaagc catgccagca
    10861 gtgccttcct tggggaaaga ggtggcctct ggagcaatgc ttcgctaccc tacatctggg
    10921 tctgtccttg cccaactcct aagctgcagg atcgctgagg agtccacatt gccatatctg
    10981 ccacttacag agtgcctact gtgcacccag cccacattca ctagtgtcct caaaacagcc
    11041 ttgagaggaa accaacgagg aaacagagtt acagttcagt taagcaactt acctaacttg
    11101 tggctaatgc atggtaggat taaacataag cctgacatat gctagccaca tgaccctccc
    11161 tttccaggag cctgctggca ttgatacagg ttgacaaaga ccagtcctac tccgttgtct
    11221 gccattactc cagccaagtc cagggacacg tgaggcaagg ggagctgggg ctgctggtgt
    11281 ggccgagacc tcagcctcag caagatggtg ccccaagctc tcagggtgcc ctgtggtccc
    11341 cttcattcat aacattgatc atgtttgtaa ctgtagttga ttctcactca tcgcagtagt
    11401 tacatcttat aaagtcacct ctgacactga gttagcgcat actgaactac tgatcctagg
    11461 ggaaatacag ggttaggttc ctgccagctt ctggtcacaa cattttcatt accaatcagt
    11521 gcataacctt gttttatgtg tgcttctgtt taaagatgcc taatttacta tatattcttg
    11581 attcgttaac attgaactca gccaacagct ctataactca tgactgaatg aagcttatct
    11641 agcacacata ttttctctgc aaggcatatc agagccttct gtgcttaaga gcacaagatg
    11701 gcacttcagc gtcatgcctg gggagcattt taagcagcca aatcaccaac acaaagcaca
    11761 aacatgcaca aagcatgatg ctaaacagac tgtgagaagg atgtttgtgt ataggatgag
    11821 aactgaacca cgaaagcaga gcatcatctt gttcgacctc agttgagaac atgttgtcgg
    11881 gccactcaga cttttcactg ctctgcgcat atcacgaatg aagtgaatgc accatgagta
    11941 ttgattttgg gtgatagaca aattttagtg aataggcaat ttcgtaaata cagaatccac
    12001 ggagggtgag gatccaccgt atgtacttat ctatgcctct aattatttgg tttccaccaa
    12061 agcacttgtt caccagatta tcttataatc ccaaaggccc agcaaagaca ggtgcttgtt
    12121 caatgtctgt tgagcgactg aaggacaaca cattcctggc ctccccaccc gcccccagcg
    12181 ggacctagca catggcctgc tgttgacact ccatgtgtct ttgttgatag aattcataaa
    12241 gacagatggg aaaaggggca cccctcttgc gggttcctga tgctgagagt tgttggaatg
    12301 ttgatgctgc ccccagagaa gggtgacagc atgcagacag actggaaacc cagaacctcc
    12361 tggggatatt cagatggtgt cactgggctc ggcctgtggg ctctcagcac atctaggtac
    12421 tggggcccag ggtgactctg agtggctcaa gtcccctggc cagatcctct ccagatgggc
    12481 ccctggccat cttagccaac atcacctgat tctgtttcct ctgactctgt cctcaaaagg
    12541 cagcatctga ttgtgatttg gtccagaaat tcctggccag aatatttcca gtgtggtgca
    12601 tgtgaacttc ccgccttccc ttccttccct cctccctcct ttcttcactg gggagaaaga
    12661 atttggcatt cgagtatctc accccgtctt gctaagcagt ttagttagac tgcaggccag
    12721 tctctgtggt gtgaccaccc cccagtggca aggagctgcc aaaactgagc ttctgggcaa
    12781 gacgtgggca gagcaaagag agacccacac ttggcacttc ctgcccagaa aggacctgct
    12841 caggcctggc tggcctttac caccgtcatc tccaggcttt ctgttcatcc gtactttcaa
    12901 agatttagtg atcaagtact atgtgtcagg aaccatgctc agcactcgat aaaacagaca
    12961 tcatctctac ctacctttgt ggagctttaa tctagtgtgg gggacggggg gactgcttcc
    13021 acccagaaac gacattttta tccactgcga taacttctgg ggagacatgg gagggaataa
    13081 cagaggggac atgatggaga tgggggactg cagagggact ctccaggaag ccacatttag
    13141 ggggtgacct caagggtgag gaagaattag ccaggtggaa agttagaggg agcagctggt
    13201 acaagggcct caagggtgga tggagggtga acgcttgatg aactggtgca ggcctgtatg
    13261 gccgggtaca gctggcaacg aggaggcccg gtatgggcag aatctggggg cctgaatggt
    13321 ggagagtctg cttgtcaccc caagagacac atcaggaagc taggtggtca gtcgggagag
    13381 agaggatgtg acactctagc caccaccacg cagagaaggg attggaaaca gtagcccgga
    13441 gaggaggctc tcttggtagg atcccacggc aaccgaatgg atgcacgttg tttatgacgt
    13501 tcttacccat gaaagcgctc aaggatccgc ttgcttgctt acatgtggga tcagcactac
    13561 cttccgtgct gccgaacatc ttcttaagcg agggaaagaa caaaaagaaa agttggcaag
    13621 aaagaaaggg caaagacaga gagactggga gagaaagagg ccccaatttc ctctctctgg
    13681 tagttgctga attggctctc gtgaaggcag aaagggaagg gctgacttat gtccctctgc
    13741 caggagaggg gaaaggaggg aggcatgata ttgacttaaa cgtgggggat ttaaaaggcc
    13801 cctatgttcc cactttgtgc tttgcttcta acagacagcc tgggaccttt gaagtcggtc
    13861 tggcccagtg gggagcgggc ttctgtggag acaagatgct aacgggggcg ggggaagggg
    13921 gccagggggc ggccgaaact ctattaggca tgctctagca aaaagaaagg caaatccaat
    13981 gataatgtac atctgtttca gggaatttat aagctttgaa cattttttat gggcagataa
    14041 gtgggtcctt ttcttaccat aacttagaac ttcaaagctg agtgtgtgga taatgcaaat
    14101 tgctgaaata tgagattttc cctacaaaga ggtttccaaa aaagacgagg aagaaaaaga
    14161 gggggggggg agagagaacg aaggctaact gatgccacag tcttttatgt acatggcaaa
    14221 aacgggacag aaattgattt ttatgaaaga agaaattgat gggagacatg gcgtggggag
    14281 gaggcggcct tgtggcaggt gctgggaggc agggactggg gccgggcggg tccacccagg
    14341 ctgtgggtca gccctgcagg gttgagctgc ctggctgtgg ctcaggccct ggggacacag
    14401 tgccctccct ctaggagcct taatggggaa aacaagaaca ctgtcactcg taatgggcat
    14461 gaaaggacac ttcagagcca gccaaggcag tgtcagccca gaacaacacc ctggaacacc
    14521 caagggggct ccaaggcagg atgggacccc ctggtttggg aaaatcagaa aaggcctcct
    14581 ggaggagagg ggctctaggg ggcaggtatg atcctgaagg gaagacagtg gggtgggaca
    14641 tttcagatgg caaaacagct gggtcaggat atccccaggg tcatgcctct gccacccaga
    14701 ccccaccaca ttcagccctc tgtgcttttc ttgtctccca catccccagc cccagtaact
    14761 agcagggaca cagtcaacat tcaatatgtg catgttgaat gaatgaatga atgaatgtga
    14821 gatcgtgaga acacacagct ggttggcaag cagcatcaac cagctctttc ttttcactat
    14881 attacatatc tacagtggta gagtcatact agaagacatg atgggatggt ggtgtggtct
    14941 ctgtagttga ccccatcatc ctttctgtcc ataggtgatt cgtctaagcc agacatggca
    15001 aagtcctcac ctttgccagt gactgattca ggaaagatca tgtgtgacct tattctgggc
    15061 aatgagatct gaagggaagt ctgttgagga acttgtaaca tgtcatggta gtggccatct
    15121 tgctacagcc tgaggaccac agagcacaca cagagagtgc tgggtccttg ggaatgatgt
    15181 tgagcaagca cttatacatg acctggcagc cacccagcat ctgtacttta gttatttggt
    15241 ttttctcagg aggagatcct gagatgaaga ctcaggagca ggttgttgat tttgaggtgg
    15301 tcctgggaaa gacagggagg agagtgggga agtgaggcag aaaaggaagg gaagttccac
    15361 tccaggagcc cacgtggaac atacctcaga gttatcccac ctgcacaggg agggagctga
    15421 ggtatttacc cacccatcct tgtcagtcat tggtcaaggg ctatttctgt gcataggcat
    15481 aggcttcagc agaattgcag gtactggcag tcggaaattg gaccagcaaa cagaaatgat
    15541 tcctacaggg gatacaagcc aggcacaggc agtgtctgtt acttggggtt cctgttgctt
    15601 agagttgaaa gcatcataac ccatcccagg tgatcataga aggacctcaa aggaaaaggt
    15661 gaggggtttg gacatttcct gagaatccat gggggaacca ttcagggttt ggggcaggtg
    15721 tcaaccacaa gaacattaaa caggctcttt tggcagcaaa gttgggagtg gtgttgaagt
    15781 aactgggaaa actccacaga ggctcagcgt ccacctctac ctgacaccct gccagcaacc
    15841 tgggtgattt ccgcaggtgt ctgaaccccg atctctcagt gttatggccc ccaccaccag
    15901 gaaggcctcc ttttctctct tctatagcca ctcactcctg ggccccactg tgaacccaga
    15961 aatgccacac catggaagcc acacactctg ctgtctcctt ctgtcctcat tcctgtcctt
    16021 ctcacagtca gtccctcttg gctcttccta gagtcccttt cattccctca tttccacttc
    16081 ctgccgctgt actgtcacct gtggcctgga tttgcactct tggtccaaca ccctcaactc
    16141 caacacctct gtctttctgc cccatccact agacaaaagc tgactctgga aaacattagg
    16201 cactcagaat caagggttct ggggtcagat ggataattgc catcatcctc accaagttgc
    16261 cactggactt tcttgcccct aaatccactg ggcatttcat tgctaccttt cttgacttct
    16321 tgattgtttt tgtgatactg acacatcccc cctttcagaa caccctctgc ccttggattc
    16381 tgtgcacagg aagctagttg ctcccctgaa tacactcttt cttccttgta atacagcctc
    16441 tgattttgag cccaagaata aagactacag ttctcagact ccttcgcaaa taaattttgt
    16501 gactaaactc tagtcaacag taagtgtcat gtagcagctc ctgggaatct cctttaaaaa
    16561 gagagcttgt ttatacctat tgtcatctct gttcttctgt gccccttctt ccattttgct
    16621 gcctggaaag cagatgtgat ggctggaatt ccagtcacca ttttggacca tgaggacaac
    16681 accctagaga tgtggagtgg ctaaaagaag cctgtgttcc tgagaactta gaggaccagg
    16741 acctctattc caggcttgga cacctacatt tagactatta tatgaggaag caatcaactt
    16801 ctcacttgtt tcaaccactt tcacttgcag tcaaacctga attgtaagtg aaattgcttt
    16861 cctgatagca aacctgttgg attttctcca gaatccctgg gccactttta gcagtcagat
    16921 tcgtctaatc ctcctttaaa gatggtggca gtgaaactgg tacatgggac ctgactgggc
    16981 tttgtttgca actttctgat aatttataat tatttcaaaa taaaaaaatt ttaaaaata
//
