LOCUS       NM_024528               5942 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens NFKB activating protein (NKAP), mRNA.
ACCESSION   NM_024528
VERSION     NM_024528.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5942)
  AUTHORS   Xu,X., Gao,C., Ye,F., Peng,A., Xu,J., Jin,K., Zhang,J., Ye,Y.,
            Yang,Y., Zhang,X., Shen,S. and Jin,F.
  TITLE     From phenotype to mechanism: Prenatal spectrum of NKAP
            mutation-related disorder and its pathogenesis inducing congenital
            heart disease
  JOURNAL   J Cell Mol Med 28 (8), e18305 (2024)
   PUBMED   38647244
  REMARK    GeneRIF: From phenotype to mechanism: Prenatal spectrum of NKAP
            mutation-related disorder and its pathogenesis inducing congenital
            heart disease.
REFERENCE   2  (bases 1 to 5942)
  AUTHORS   Sun,S., Gao,T., Pang,B., Su,X., Guo,C., Zhang,R. and Pang,Q.
  TITLE     RNA binding protein NKAP protects glioblastoma cells from
            ferroptosis by promoting SLC7A11 mRNA splicing in an m6A-dependent
            manner
  JOURNAL   Cell Death Dis 13 (1), 73 (2022)
   PUBMED   35064112
  REMARK    GeneRIF: RNA binding protein NKAP protects glioblastoma cells from
            ferroptosis by promoting SLC7A11 mRNA splicing in an
            m(6)A-dependent manner.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 5942)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 5942)
  AUTHORS   Liu,J., Chen,S.J., Hsu,S.W., Zhang,J., Li,J.M., Yang,D.C., Gu,S.,
            Pinkerton,K.E. and Chen,C.H.
  TITLE     MARCKS cooperates with NKAP to activate NF-kB signaling in
            smoke-related lung cancer
  JOURNAL   Theranostics 11 (9), 4122-4136 (2021)
   PUBMED   33754052
  REMARK    GeneRIF: MARCKS cooperates with NKAP to activate NF-kB signaling in
            smoke-related lung cancer.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5942)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 5942)
  AUTHORS   Hegele,A., Kamburov,A., Grossmann,A., Sourlis,C., Wowro,S.,
            Weimann,M., Will,C.L., Pena,V., Luhrmann,R. and Stelzl,U.
  TITLE     Dynamic protein-protein interaction wiring of the human spliceosome
  JOURNAL   Mol Cell 45 (4), 567-580 (2012)
   PUBMED   22365833
REFERENCE   7  (bases 1 to 5942)
  AUTHORS   Hsu,F.C., Pajerowski,A.G., Nelson-Holte,M., Sundsbak,R. and
            Shapiro,V.S.
  TITLE     NKAP is required for T cell maturation and acquisition of
            functional competency
  JOURNAL   J Exp Med 208 (6), 1291-1304 (2011)
   PUBMED   21624937
  REMARK    GeneRIF: NKAP is required for T cell maturation and the acquisition
            of functional competency
REFERENCE   8  (bases 1 to 5942)
  AUTHORS   D'Addabbo,A., Latiano,A., Palmieri,O., Creanza,M.T., Maglietta,R.,
            Annese,V. and Ancona,N.
  TITLE     Association of genetic profiles to Crohn's disease by linear
            combinations of single nucleotide polymorphisms
  JOURNAL   Artif Intell Med 46 (2), 131-138 (2009)
   PUBMED   18804983
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
REFERENCE   9  (bases 1 to 5942)
  AUTHORS   Pajerowski,A.G., Nguyen,C., Aghajanian,H., Shapiro,M.J. and
            Shapiro,V.S.
  TITLE     NKAP is a transcriptional repressor of notch signaling and is
            required for T cell development
  JOURNAL   Immunity 30 (5), 696-707 (2009)
   PUBMED   19409814
  REMARK    GeneRIF: NKAP functions as a transcriptional repressor, acting on
            Notch target genes, and is required for alphabeta T cell
            development.
REFERENCE   10 (bases 1 to 5942)
  AUTHORS   Chen,D., Li,Z., Yang,Q., Zhang,J., Zhai,Z. and Shu,H.B.
  TITLE     Identification of a nuclear protein that promotes NF-kappaB
            activation
  JOURNAL   Biochem Biophys Res Commun 310 (3), 720-724 (2003)
   PUBMED   14550261
  REMARK    GeneRIF: NKAP is a novel nuclear regulator of TNF- and IL-1-induced
            NF-kappaB activation
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AY388958.1, BC015354.1 and
            AC002477.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 22, 2018 this sequence version replaced NM_024528.3.
            
            Summary: This gene encodes a protein that is involved in the
            activation of the ubiquitous transcription factor NF-kappaB. This
            protein is associated with the the histone deacetylase HDAC3 and
            with the Notch corepressor complex, and it thereby acts as a
            transcriptional repressor of Notch target genes. It is also
            required for alphabeta T cell development. A related pseudogene has
            been identified on chromosome X, while a related and intronless
            retrocopy, which has an intact CDS and may be functional, is
            located on chromosome 6. [provided by RefSeq, May 2010].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AY388958.1, AK026279.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000371410.5/ ENSP00000360464.3
            RefSeq Select criteria :: based on single protein-coding transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1554              AY388958.1         22-1575
            1555-1564           BC015354.1         1440-1449
            1565-5942           AC002477.1         97197-101574        c
FEATURES             Location/Qualifiers
     source          1..5942
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xq24"
     gene            1..5942
                     /gene="NKAP"
                     /gene_synonym="MRXSHD"
                     /note="NFKB activating protein"
                     /db_xref="GeneID:79576"
                     /db_xref="HGNC:HGNC:29873"
                     /db_xref="MIM:300766"
     exon            1..532
                     /gene="NKAP"
                     /gene_synonym="MRXSHD"
                     /inference="alignment:Splign:2.1.0"
     CDS             147..1394
                     /gene="NKAP"
                     /gene_synonym="MRXSHD"
                     /note="NF-kappaB activating protein"
                     /codon_start=1
                     /product="NF-kappa-B-activating protein"
                     /protein_id="NP_078804.2"
                     /db_xref="CCDS:CCDS14592.1"
                     /db_xref="GeneID:79576"
                     /db_xref="HGNC:HGNC:29873"
                     /db_xref="MIM:300766"
                     /translation="MAPVSGSRSPDREASGSGGRRRSSSKSPKPSKSARSPRGRRSRS
                     HSCSRSGDRNGLTHQLGGLSQGSRNQSYRSRSRSRSRERPSAPRGIPFASASSSVYYG
                     SYSRPYGSDKPWPSLLDKEREESLRQKRLSERERIGELGAPEVWGLSPKNPEPDSDEH
                     TPVEDEEPKKSTTSASTSEEEKKKKSSRSKERSKKRRKKKSSKRKHKKYSEDSDSDSD
                     SETDSSDEDNKRRAKKAKKKEKKKKHRSKKYKKKRSKKSRKESSDSSSKESQEEFLEN
                     PWKDRTKAEEPSDLIGPEAPKTLTSQDDKPLNYGHALLPGEGAAMAEYVKAGKRIPRR
                     GEIGLTSEEIASFECSGYVMSGSRHRRMEAVRLRKENQIYSADEKRALASFNQEERRK
                     RENKILASFREMVYRKTKGKDDK"
     exon            533..613
                     /gene="NKAP"
                     /gene_synonym="MRXSHD"
                     /inference="alignment:Splign:2.1.0"
     exon            614..684
                     /gene="NKAP"
                     /gene_synonym="MRXSHD"
                     /inference="alignment:Splign:2.1.0"
     exon            685..819
                     /gene="NKAP"
                     /gene_synonym="MRXSHD"
                     /inference="alignment:Splign:2.1.0"
     exon            820..883
                     /gene="NKAP"
                     /gene_synonym="MRXSHD"
                     /inference="alignment:Splign:2.1.0"
     exon            884..993
                     /gene="NKAP"
                     /gene_synonym="MRXSHD"
                     /inference="alignment:Splign:2.1.0"
     exon            994..1069
                     /gene="NKAP"
                     /gene_synonym="MRXSHD"
                     /inference="alignment:Splign:2.1.0"
     exon            1070..1219
                     /gene="NKAP"
                     /gene_synonym="MRXSHD"
                     /inference="alignment:Splign:2.1.0"
     exon            1220..5942
                     /gene="NKAP"
                     /gene_synonym="MRXSHD"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1535..1540
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NKAP"
                     /gene_synonym="MRXSHD"
                     /note="hexamer: AATAAA"
     polyA_site      1559
                     /gene="NKAP"
                     /gene_synonym="MRXSHD"
                     /note="major polyA site"
     regulatory      5922..5927
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NKAP"
                     /gene_synonym="MRXSHD"
                     /note="hexamer: AATAAA"
     polyA_site      5942
                     /gene="NKAP"
                     /gene_synonym="MRXSHD"
ORIGIN      
        1 gcagaagtac ccagaactgt gtccaaggtt tcctcagatt tgggctgttc cgcagcggca
       61 ggtcccggga accaaggcaa cagacatctt cctaggctcg cgagagcgcc cccttgtccc
      121 acggctgctg gggcccccca gtagccatgg ctccggtgtc cggctcacgc agcccggata
      181 gggaggcctc gggctcgggg ggaagacgtc gcagttcgtc gaagagtccg aagcccagca
      241 aatctgcccg ctccccgcgg ggccgccgct ctcgctcgca ctcttgctct cggtccgggg
      301 accggaatgg actcacccat cagctgggtg gcctcagcca aggctcccga aaccagtcct
      361 accgctcacg ctcgcggtcg cgttctagag agcggccctc tgcgccccgg ggcatcccct
      421 tcgcttctgc ctcctcgtca gtctattacg gcagctactc gcgcccctac gggagcgaca
      481 agccttggcc tagcctcctc gacaaggaga gggaggagag cctgcggcag aagagattaa
      541 gtgagagaga gagaattgga gaattgggag ctcctgaagt atggggactt tctccaaaga
      601 atcctgaacc agattctgat gaacatacac cagtggagga tgaagagcca aagaaaagca
      661 ctacttcagc ttctacttca gaagaagaaa aaaagaagaa gtctagccgt tcaaaagaaa
      721 ggtccaagaa aaggagaaag aaaaaatcat cgaaaagaaa acataagaag tattctgaag
      781 atagcgacag tgactctgat tctgaaacag actccagtga tgaagataac aaaaggagag
      841 caaagaaagc caagaaaaag gaaaagaaga agaaacacag atcgaagaaa tataagaaaa
      901 agaggtctaa gaagagcaga aaagagtcca gtgattcaag ctctaaagaa tcccaagaag
      961 agtttctgga aaatccctgg aaggatcgaa caaaggctga agaaccatca gatttaattg
     1021 gcccagaggc tccaaaaaca cttacctctc aagatgataa acctttgaac tatggccatg
     1081 ctctgttacc tggtgaaggt gcagctatgg ctgaatatgt aaaagctgga aaacgtatcc
     1141 cacgaagagg tgaaattggc ttgacaagtg aagaaattgc atcatttgaa tgctcaggtt
     1201 atgtaatgag tggtagcagg catcgccgaa tggaggctgt gcgactgcga aaagagaacc
     1261 agatctacag tgctgatgag aagagagccc ttgcatcctt taaccaagaa gagagacgaa
     1321 agagagagaa caagattctg gccagttttc gagaaatggt ttacagaaag accaaaggga
     1381 aggatgacaa ataaagattt tctgattgtc cagaagacat ttttaacaac aaaaaagaaa
     1441 gtctgggttc cacacataca tagaaaaaga ttattatgtt ctgagaaagc tttacagtgc
     1501 tactgtgcct tctatttaat tctttcagtc cttcaataaa aagctgctta ttgatataac
     1561 tttagcaagt tctttgggtt attttggatt gaccatagta actttctggt ttaaaaatcc
     1621 aaattatggg ctgggcacgg ttgctcacgc ctgtaatccc agcactttgg gaggctgagg
     1681 cgggtggatc acttgaggcc aggagttcga gaccagcctg gccaacatgg tgaaaccctg
     1741 tccctactga aaatacaaaa tcagccagga gtggtggcgc acacctgtag tcccagctac
     1801 tcgagaggaa gaggcaggag aattgcttga acctaggagg tggaggttgc agtgagccga
     1861 gattgtgcca ctgcactcca gcctgggcga caaagcaaga ctgtgtctca gaaaaaaata
     1921 aaaggccagg cacggtggct cacgcctgta atcccagcac tttgggaggc caaggcgggt
     1981 ggatcacctg aggtcaggag tcgagaccag cctggccaac atggtgaaac cctgtctcta
     2041 ccaaaaaata caaaaattag ccgggcgtgg tggtgggcgc ctgtaatccc aactactcgg
     2101 gaggctgagg caggagaatt gcttgaatcc aggaggcgga ggttgcagtg agccgagatt
     2161 gtgacattgc actccagcct gggcaagaag agtgaaactc tgtctcaaaa aaaaaaaatc
     2221 caaattatga ataaaatgct actgtattgg aggaggtggc ttgaacaaaa agtgttagtg
     2281 agatgagaaa cttcaatctc acaccatgag acagtgctac tattagctct atgtgatgcc
     2341 tgccccaccc cgacctctac cccagctgaa cttttgggaa attatcactc ctctgtgttg
     2401 aatgtgttat aaatggactt gataggaaca tataggaggc cacttttgtt aacaaaatga
     2461 aggcattgct gcgaaaagca aatctggaat ttgaaaactg acttgtgtac tataatatat
     2521 gatctaccct ggaaataatg ggttttgatt taagtcccta cctccactct aaaaccaaca
     2581 aacaaaccaa aaagcaaact aacccacaga tttgttgaat tgaaaatgtg aacattagat
     2641 attttaaatt gttttttttg agatggaatc tcgctcttgt tgcccaggct ggagtacaat
     2701 ggggtgatct cggctcacca caacctccac ctcctaagtt caagcagttc tcctgcctca
     2761 gcctcctgag tagctgggat tacaggcatg tgccaccatg cctggctaat tttgtatttt
     2821 tagtagagat ggggtttctc catgttggtc aggctggtct cgaactccca accccaggtg
     2881 atccgcccgc cttggcctcc caaagtgctg ggattacagg catgagccac tgcgtccagc
     2941 ctaaattctt aataagttac ttaaaaatag ttatttgaaa tataaagtct taagaagtct
     3001 actcttatcc ccatgtaaat actttatttc aatctgagct gatatgagct tgaggatagg
     3061 ataacatcca aaagaatagg agtcaactat ggtcaagagt tttgataagg aaaaatataa
     3121 tgagtcagta ttttagctta attgtttcat aagagaaact aaaatgatat taaatatttt
     3181 taactgtctt tcatatacta agtagatttc attttaaaca cttaaacctt tctgtttgtt
     3241 tttaagttgg ggagaaatgg aaaattgtgg gactatgata tcgtggtctt gttcctttgt
     3301 ttatttcgtt caattttaat taggtcagaa gagtgaatgg gggagggaac ccaggaagtt
     3361 ttggctgttt tcattaaggt tggttttctg ttttttgttt tttgagatag agtctcactc
     3421 tgtcactcag gctggagccc agtggtgtga tctcggctca ctgtaacctc tgcctcctgg
     3481 gttcaagtga tcctcccacc tcagcctccc aagtagctgg gattacaggc actcaccacc
     3541 acaactggct aatttttgta ttttcaatag agatggggtt tcgccatctt ggccaggctg
     3601 gtctcgaact cctgacctca agtgatctgc ccgcctcagc ctcctgaaat gctgggatta
     3661 caggtgtgag caaccgtgcc tggccgtttt tgttaaggtt atttgatctg cattattatt
     3721 acatgcctat gataaattta aattacctga gtgataagga cattctgatt atactattgt
     3781 aaaatgcccg cttgccttat atttttaagt ggttgttttt caaaagtgtt taaataaggc
     3841 agtcatattt caaagtatag agcaaaagtt tttttattat tatttttgag acagggtctc
     3901 cctctgtcac ccaggctaga gttcagtggt gcaatctcgg ctcactgcaa cctccacctc
     3961 ctgggctcaa gcaatcctcc acctcagcct cccgagcagc tgggactacc agtgtgcacc
     4021 accatgccca gctaattttt gtatagttag tagagacaag gtttcacctt attgcccagg
     4081 ctggtctcaa actccagacc tcaaacaatc cgcccgcctc ggcctcccaa agtgctagga
     4141 ttataggcgt gagccaccac acccggccca aagttttata attaaacagt attaacagtc
     4201 ttaaatacta ttaaatatat gttccaggaa atgtctactt ttaacccgca actactgaaa
     4261 ataagagtca gcatgtagca gcattaatga cccctgaatt tgaaatgatt tctgtttatt
     4321 tatacctaat tgtagttgct gcctagccta ataaatctgt agttttcaaa agttaaaaga
     4381 aactagttcc caaagttttg agtctcaatc catctacttc cctgaataat aaaacctgaa
     4441 ttttgtaata cccacttaaa aattttattc tcagctgggc gtggtggctc acacctataa
     4501 tcccagcact ttgggaggcc gaggtgggcg gaccatgagg tcaggagatc aagaccatcc
     4561 tggctaacat ggtgaaaccc catctctact aaaaatacaa aaaatgtagc cgggcatggt
     4621 ggcacgtgcc tgtagtccca gctactcggg aggctgaggc aggagaattg cttgaacctg
     4681 ggaggtggag gttgcagtga gccgagatca agccactgca ctccggcctg ggagacagaa
     4741 tgagactccg tctctaaata aacaaacaaa taaaaatgta ttctctaagg acattttttt
     4801 tttggttact ggttcctaga gatgaaatga ttatcttatt ttcagtaggc aaggtagttt
     4861 aaatgcagaa attctgcgat atatgaatat tgtgacatca agaacagctt cattggcata
     4921 ttctttggga aagaggcaag aagaataaaa gggctaaaag tattttattt tcagcttatg
     4981 agtgttgtta ctgcttcaaa ttgtgtttag tcacctgagc cataaatcag ttgtagtagg
     5041 gctattgtat tttaaatata ttttttgttc tccaaggaga cttatctagt ttgccacctc
     5101 cttgcctatt gctctgatgt ggttttccct ccttttttat ttattgcaca aatatctaac
     5161 agttaaaaaa gaaaaatttc agtaccatat caattgtctt tcatattttg tgttcctcct
     5221 ttccagtgct tttctatgta agtaaatatt ctgatgtact tgtaagtaat cactcagagc
     5281 ataatgtaat tttgtctttg gtgtcttaaa attttgcagc caggcaagag aggttcacag
     5341 ggaaggtagg aacaagtgat tattcagtgt gaggatggga agaagatcaa agataagtaa
     5401 gggcaatgat tgtatccttc ccgtgtcatt gcccctagtc cccaagccac cctcctgcaa
     5461 tcatgagtat agtggtgtat tgtggaaggc agcatgccca gttttccagt ttcaaaatat
     5521 attatttagc agtgaggctt aactcccagt aaatgaaggg gtctgtgagt aggggaaccc
     5581 ttcagactgg ttatattatg agctgtagaa ctgttgccat tttgttgttc ccagttgtgc
     5641 ccataaagag atgtgtctga gctggagact taggctcaga tggggaacaa acagccaagt
     5701 accaacctta gtttggtcca ataggtgaat tttagcaact ctctaaactc agctctatac
     5761 ctgtaagact cagaagtgat acatatagtg atagaaaaaa atactttaga aaatagaaaa
     5821 caacttactg gttactctgc taatgtgatg gaggttgcag aatggaaaat gaatgaatgc
     5881 aaaaaaatac agctggattg tgtgccacaa gtttattact caataaatat tctgtggttt
     5941 ga
//
