LOCUS       NM_007184               5139 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens nischarin (NISCH), transcript variant 1, mRNA.
ACCESSION   NM_007184
VERSION     NM_007184.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5139)
  AUTHORS   Zheng,P., Pan,C., Zhou,C., Liu,B., Wang,L., Duan,S. and Ding,Y.
  TITLE     Contribution of Nischarin/IRAS in CNS development, injury and
            diseases
  JOURNAL   J Adv Res 54, 43-57 (2023)
   PUBMED   36716956
  REMARK    GeneRIF: Contribution of Nischarin/IRAS in CNS development, injury
            and diseases.
            Review article
REFERENCE   2  (bases 1 to 5139)
  AUTHORS   Ostojic,M., Jevric,M., Mitrovic-Ajtic,O., Zivic,K., Tanic,M.,
            Cavic,M., Srdic-Rajic,T. and Grahovac,J.
  TITLE     Nischarin expression may have differing roles in male and female
            melanoma patients
  JOURNAL   J Mol Med (Berl) 101 (8), 1001-1014 (2023)
   PUBMED   37382661
  REMARK    GeneRIF: Nischarin expression may have differing roles in male and
            female melanoma patients.
REFERENCE   3  (bases 1 to 5139)
  AUTHORS   Yang,Z.H., Cai,X., Ding,Z.L., Li,W., Zhang,C.Y., Huo,J.H.,
            Zhang,Y., Wang,L., Zhang,L.M., Li,S.W., Li,M., Zhang,C., Chang,H.
            and Xiao,X.
  TITLE     Identification of a psychiatric risk gene NISCH at 3p21.1 GWAS
            locus mediating dendritic spine morphogenesis and cognitive
            function
  JOURNAL   BMC Med 21 (1), 254 (2023)
   PUBMED   37443018
  REMARK    GeneRIF: Identification of a psychiatric risk gene NISCH at 3p21.1
            GWAS locus mediating dendritic spine morphogenesis and cognitive
            function.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 5139)
  AUTHORS   Zhao,X., Chen,L., Wu,J., You,J., Hong,Q. and Ye,F.
  TITLE     Transcription factor KLF15 inhibits the proliferation and migration
            of gastric cancer cells via regulating the TFAP2A-AS1/NISCH axis
  JOURNAL   Biol Direct 16 (1), 21 (2021)
   PUBMED   34727954
  REMARK    GeneRIF: Transcription factor KLF15 inhibits the proliferation and
            migration of gastric cancer cells via regulating the
            TFAP2A-AS1/NISCH axis.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 5139)
  AUTHORS   Liu,X., Salokas,K., Tamene,F., Jiu,Y., Weldatsadik,R.G., Ohman,T.
            and Varjosalo,M.
  TITLE     An AP-MS- and BioID-compatible MAC-tag enables comprehensive
            mapping of protein interactions and subcellular localizations
  JOURNAL   Nat Commun 9 (1), 1188 (2018)
   PUBMED   29568061
  REMARK    Publication Status: Online-Only
REFERENCE   6  (bases 1 to 5139)
  AUTHORS   Piletz,J.E., Deleersnijder,W., Roth,B.L., Ernsberger,P., Zhu,H. and
            Ziegler,D.
  TITLE     IRAS splice variants
  JOURNAL   Ann N Y Acad Sci 1009, 419-426 (2003)
   PUBMED   15028621
  REMARK    GeneRIF: Results describe three alternatively spliced transcripts
            of the human I(1)-imidazoline receptor candidate gene, IRAS.
REFERENCE   7  (bases 1 to 5139)
  AUTHORS   El-Ayoubi,R., Gutkowska,J., Regunathan,S. and Mukaddam-Daher,S.
  TITLE     Imidazoline receptors in the heart: characterization, distribution,
            and regulation
  JOURNAL   J Cardiovasc Pharmacol 39 (6), 875-883 (2002)
   PUBMED   12021582
  REMARK    GeneRIF: The heart possesses imidazoline I1-receptors that are
            up-regulated in the presence of hypertension or heart failure,
            which suggests their involvement in cardiovascular regulation.
REFERENCE   8  (bases 1 to 5139)
  AUTHORS   Alahari,S.K., Lee,J.W. and Juliano,R.L.
  TITLE     Nischarin, a novel protein that interacts with the integrin alpha5
            subunit and inhibits cell migration
  JOURNAL   J Cell Biol 151 (6), 1141-1154 (2000)
   PUBMED   11121431
REFERENCE   9  (bases 1 to 5139)
  AUTHORS   Piletz,J.E., Ivanov,T.R., Sharp,J.D., Ernsberger,P., Chang,C.H.,
            Pickard,R.T., Gold,G., Roth,B., Zhu,H., Jones,J.C., Baldwin,J. and
            Reis,D.J.
  TITLE     Imidazoline receptor antisera-selected (IRAS) cDNA: cloning and
            characterization
  JOURNAL   DNA Cell Biol 19 (6), 319-329 (2000)
   PUBMED   10882231
REFERENCE   10 (bases 1 to 5139)
  AUTHORS   Ivanov,T.R., Jones,J.C., Dontenwill,M., Bousquet,P. and Piletz,J.E.
  TITLE     Characterization of a partial cDNA clone detected by imidazoline
            receptor-selective antisera
  JOURNAL   J Auton Nerv Syst 72 (2-3), 98-110 (1998)
   PUBMED   9851558
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC006208.3.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_007184.3.
            
            Summary: This gene encodes a nonadrenergic imidazoline-1 receptor
            protein that localizes to the cytosol and anchors to the inner
            layer of the plasma membrane. The orthologous mouse protein has
            been shown to influence cytoskeletal organization and cell
            migration by binding to alpha-5-beta-1 integrin. In humans, this
            protein has been shown to bind to the adapter insulin receptor
            substrate 4 (IRS4) to mediate translocation of alpha-5 integrin
            from the cell membrane to endosomes. Expression of this protein was
            reduced in human breast cancers while its overexpression reduced
            tumor growth and metastasis; possibly by limiting the expression of
            alpha-5 integrin. In human cardiac tissue, this gene was found to
            affect cell growth and death while in neural tissue it affected
            neuronal growth and differentiation. Alternative splicing results
            in multiple transcript variants encoding differerent isoforms. Some
            isoforms lack the expected C-terminal domains of a functional
            imidazoline receptor. [provided by RefSeq, Jan 2013].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1; also known as
            IRAS-M).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC038102.2, AF082516.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000345716.9/ ENSP00000339958.4
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-131               AC006208.3         64044-64174
            132-215             AC006208.3         66283-66366
            216-398             AC006208.3         67102-67284
            399-447             AC006208.3         79299-79347
            448-611             AC006208.3         80254-80417
            612-707             AC006208.3         80743-80838
            708-803             AC006208.3         82174-82269
            804-956             AC006208.3         84887-85039
            957-1025            AC006208.3         86014-86082
            1026-1211           AC006208.3         86537-86722
            1212-1340           AC006208.3         86889-87017
            1341-1454           AC006208.3         88189-88302
            1455-1566           AC006208.3         88624-88735
            1567-1691           AC006208.3         92953-93077
            1692-1741           AC006208.3         94218-94267
            1742-3151           AC006208.3         95636-97045
            3152-3494           AC006208.3         97776-98118
            3495-3651           AC006208.3         98515-98671
            3652-3780           AC006208.3         99145-99273
            3781-3942           AC006208.3         99792-99953
            3943-5139           AC006208.3         100312-101508
FEATURES             Location/Qualifiers
     source          1..5139
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="3"
                     /map="3p21.1"
     gene            1..5139
                     /gene="NISCH"
                     /gene_synonym="hIRAS; I-1; IR1; IRAS"
                     /note="nischarin"
                     /db_xref="GeneID:11188"
                     /db_xref="HGNC:HGNC:18006"
                     /db_xref="MIM:615507"
     exon            1..131
                     /gene="NISCH"
                     /gene_synonym="hIRAS; I-1; IR1; IRAS"
                     /inference="alignment:Splign:2.1.0"
     CDS             39..4553
                     /gene="NISCH"
                     /gene_synonym="hIRAS; I-1; IR1; IRAS"
                     /note="isoform 1 is encoded by transcript variant 1; I-1
                     receptor candidate protein; imidazoline receptor antisera
                     selected; I1R candidate protein; imidazoline receptor 1"
                     /codon_start=1
                     /product="nischarin isoform 1"
                     /protein_id="NP_009115.3"
                     /db_xref="CCDS:CCDS33767.1"
                     /db_xref="GeneID:11188"
                     /db_xref="HGNC:HGNC:18006"
                     /db_xref="MIM:615507"
                     /translation="MATARTFGPEREAEPAKEARVVGSELVDTYTVYIIQVTDGSHEW
                     TVKHRYSDFHDLHEKLVAERKIDKNLLPPKKIIGKNSRSLVEKREKDLEVYLQKLLAA
                     FPGVTPRVLAHFLHFHFYEINGITAALAEELFEKGEQLLGAGEVFAIGPLQLYAVTEQ
                     LQQGKPTCASGDAKTDLGHILDFTCRLKYLKVSGTEGPFGTSNIQEQLLPFDLSIFKS
                     LHQVEISHCDAKHIRGLVASKPTLATLSVRFSATSMKEVLVPEASEFDEWEPEGTTLE
                     GPVTAVIPTWQALTTLDLSHNSVSEIDESVKLIPKIEFLDLSHNGLLVVDNLQHLYNL
                     VHLDLSYNKLSSLEGLHTKLGNIKTLNLAGNLLESLSGLHKLYSLVNLDLRDNRIEQM
                     EEVRSIGSLPCLEHVSLLNNPLSIIPDYRTKVLAQFGERASEVCLDDTVTTEKELDTV
                     EVLKAIQKAKEVKSKLSNPEKKGGEDSRLSAAPCIRPSSSPPTVAPASASLPQPILSN
                     QGIMFVQEEALASSLSSTDSLTPEHQPIAQGCSDSLESIPAGQAASDDLRDVPGAVGG
                     ASPEHAEPEVQVVPGSGQIIFLPFTCIGYTATNQDFIQRLSTLIRQAIERQLPAWIEA
                     ANQREEGQGEQGEEEDEEEEEEEDVAENRYFEMGPPDVEEEEGGGQGEEEEEEEEDEE
                     AEEERLALEWALGADEDFLLEHIRILKVLWCFLIHVQGSIRQFAACLVLTDFGIAVFE
                     IPHQESRGSSQHILSSLRFVFCFPHGDLTEFGFLMPELCLVLKVRHSENTLFIISDAA
                     NLHEFHADLRSCFAPQHMAMLCSPILYGSHTSLQEFLRQLLTFYKVAGGCQERSQGCF
                     PVYLVYSDKRMVQTAAGDYSGNIEWASCTLCSAVRRSCCAPSEAVKSAAIPYWLLLTP
                     QHLNVIKADFNPMPNRGTHNCRNRNSFKLSRVPLSTVLLDPTRSCTQPRGAFADGHVL
                     ELLVGYRFVTAIFVLPHEKFHFLRVYNQLRASLQDLKTVVIAKTPGTGGSPQGSFADG
                     QPAERRASNDQRPQEVPAEALAPAPAEVPAPAPAAASASGPAKTPAPAEASTSALVPE
                     ETPVEAPAPPPAEAPAQYPSEHLIQATSEENQIPSHLPACPSLRHVASLRGSAIIELF
                     HSSIAEVENEELRHLMWSSVVFYQTPGLEVTACVLLSTKAVYFVLHDGLRRYFSEPLQ
                     DFWHQKNTDYNNSPFHISQCFVLKLSDLQSVNVGLFDQHFRLTGSTPMQVVTCLTRDS
                     YLTHCFLQHLMVVLSSLERTPSPEPVDKDFYSEFGNKTTGKMENYELIHSSRVKFTYP
                     SEEEIGDLTFTVAQKMAEPEKAPALSILLYVQAFQVGMPPPGCCRGPLRPKTLLLTSS
                     EIFLLDEDCVHYPLPEFAKEPPQRDRYRLDDGRRVRDLDRVLMGYQTYPQALTLVFDD
                     VQGHDLMGSVTLDHFGEVPGGPARASQGREVQWQVFVPSAESREKLISLLARQWEALC
                     GRELPVELTG"
     exon            132..215
                     /gene="NISCH"
                     /gene_synonym="hIRAS; I-1; IR1; IRAS"
                     /inference="alignment:Splign:2.1.0"
     exon            216..398
                     /gene="NISCH"
                     /gene_synonym="hIRAS; I-1; IR1; IRAS"
                     /inference="alignment:Splign:2.1.0"
     exon            399..447
                     /gene="NISCH"
                     /gene_synonym="hIRAS; I-1; IR1; IRAS"
                     /inference="alignment:Splign:2.1.0"
     exon            448..611
                     /gene="NISCH"
                     /gene_synonym="hIRAS; I-1; IR1; IRAS"
                     /inference="alignment:Splign:2.1.0"
     exon            612..707
                     /gene="NISCH"
                     /gene_synonym="hIRAS; I-1; IR1; IRAS"
                     /inference="alignment:Splign:2.1.0"
     exon            708..803
                     /gene="NISCH"
                     /gene_synonym="hIRAS; I-1; IR1; IRAS"
                     /inference="alignment:Splign:2.1.0"
     exon            804..956
                     /gene="NISCH"
                     /gene_synonym="hIRAS; I-1; IR1; IRAS"
                     /inference="alignment:Splign:2.1.0"
     exon            957..1025
                     /gene="NISCH"
                     /gene_synonym="hIRAS; I-1; IR1; IRAS"
                     /inference="alignment:Splign:2.1.0"
     exon            1026..1211
                     /gene="NISCH"
                     /gene_synonym="hIRAS; I-1; IR1; IRAS"
                     /inference="alignment:Splign:2.1.0"
     exon            1212..1340
                     /gene="NISCH"
                     /gene_synonym="hIRAS; I-1; IR1; IRAS"
                     /inference="alignment:Splign:2.1.0"
     exon            1341..1454
                     /gene="NISCH"
                     /gene_synonym="hIRAS; I-1; IR1; IRAS"
                     /inference="alignment:Splign:2.1.0"
     exon            1455..1566
                     /gene="NISCH"
                     /gene_synonym="hIRAS; I-1; IR1; IRAS"
                     /inference="alignment:Splign:2.1.0"
     exon            1567..1691
                     /gene="NISCH"
                     /gene_synonym="hIRAS; I-1; IR1; IRAS"
                     /inference="alignment:Splign:2.1.0"
     exon            1692..1741
                     /gene="NISCH"
                     /gene_synonym="hIRAS; I-1; IR1; IRAS"
                     /inference="alignment:Splign:2.1.0"
     exon            1742..3151
                     /gene="NISCH"
                     /gene_synonym="hIRAS; I-1; IR1; IRAS"
                     /inference="alignment:Splign:2.1.0"
     exon            3152..3494
                     /gene="NISCH"
                     /gene_synonym="hIRAS; I-1; IR1; IRAS"
                     /inference="alignment:Splign:2.1.0"
     exon            3495..3651
                     /gene="NISCH"
                     /gene_synonym="hIRAS; I-1; IR1; IRAS"
                     /inference="alignment:Splign:2.1.0"
     exon            3652..3780
                     /gene="NISCH"
                     /gene_synonym="hIRAS; I-1; IR1; IRAS"
                     /inference="alignment:Splign:2.1.0"
     exon            3781..3942
                     /gene="NISCH"
                     /gene_synonym="hIRAS; I-1; IR1; IRAS"
                     /inference="alignment:Splign:2.1.0"
     exon            3943..5139
                     /gene="NISCH"
                     /gene_synonym="hIRAS; I-1; IR1; IRAS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5116..5121
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NISCH"
                     /gene_synonym="hIRAS; I-1; IR1; IRAS"
                     /note="hexamer: AATAAA"
     polyA_site      5139
                     /gene="NISCH"
                     /gene_synonym="hIRAS; I-1; IR1; IRAS"
                     /note="major polyA site"
ORIGIN      
        1 agtctgcgcc gggcggcggt ggcggcggag acccgaacat ggcgaccgcg cgcaccttcg
       61 ggcccgagcg ggaagccgag ccggccaagg aagcgcgcgt cgtgggctcg gagcttgtgg
      121 acacttatac ggtttacatc atccaggtca ctgatggcag ccatgagtgg acagtaaagc
      181 accgctacag cgacttccat gacctgcatg aaaagctcgt tgcagagaga aagattgata
      241 aaaatctgct tccgcccaaa aagataattg ggaaaaactc aagaagcttg gtggagaaga
      301 gggagaagga tctggaggtc tacctccaga agctcctggc tgccttccct ggcgtgaccc
      361 ccagagtact ggcccacttc ttgcattttc acttctatga gataaatggc atcaccgcgg
      421 cactggctga agagctcttt gagaaaggag aacagctcct gggggccggc gaggtctttg
      481 ccattggacc cctgcagctg tatgccgtca cggagcagct gcagcaggga aagcccacgt
      541 gcgccagtgg ggatgccaag accgacctcg ggcacatcct ggacttcacc tgtcgcctta
      601 agtaccttaa ggtttctggc acagaaggac cttttgggac cagcaacatt caggagcagc
      661 tcctgccgtt cgacctatca atattcaagt ccctgcatca ggtggagata agtcactgtg
      721 atgctaagca catcagaggg ctggtcgcat cgaagcccac cttagccacg ctgagtgtcc
      781 gcttctcagc aacctcgatg aaggaagtcc ttgttcctga agcctcagaa tttgatgagt
      841 gggagcctga aggcacaacc ctagaaggcc ctgtgactgc cgtcatcccc acttggcagg
      901 cattgaccac gcttgacctg agccacaaca gcgtctccga gatcgacgag tctgtgaaac
      961 tgatcccaaa gattgagttc ctggacctga gtcacaatgg attgctggtt gtggacaatc
     1021 tgcagcacct gtataacctt gtgcatctgg acctgtccta caacaagctc tcctccttgg
     1081 aagggcttca caccaagctg gggaacatca agaccttaaa cctggcaggc aacctcctag
     1141 agagtctgag tggcctgcac aagctctact cactggtcaa cctggatctc cgggacaaca
     1201 ggatcgaaca gatggaggag gtccggagca taggcagcct cccgtgtctg gagcacgtgt
     1261 ctctgctgaa caaccctctg agcatcatcc ccgactaccg gaccaaggtg ctggctcagt
     1321 tcggagagag ggcctcagag gtctgtctgg atgacacagt gaccacagag aaggagctgg
     1381 acactgtgga agtgctgaaa gcaattcaga aagccaagga ggtcaagtcc aaactgagca
     1441 acccagagaa gaagggtggt gaagactccc ggctctcagc tgccccctgc atcagaccca
     1501 gcagctcccc tcccactgtg gctcccgcat ctgcctccct gccccagccc atcctctcta
     1561 accaaggaat catgttcgtt caggaggagg ccctggccag cagcctctcg tccactgaca
     1621 gtctgactcc cgagcaccag cccattgccc agggatgttc tgattccttg gagtccatcc
     1681 ctgcgggaca ggcagcttcc gatgatttaa gggacgtgcc aggagctgtt ggtggtgcaa
     1741 gcccagaaca tgccgagccg gaggtccagg tggtgccggg gtctggccag atcatcttcc
     1801 tgcccttcac ctgcattggc tacacggcca ccaatcagga cttcatccag cgcctgagca
     1861 cactgatccg gcaggccatc gagcggcagc tgcctgcctg gatcgaggct gccaaccagc
     1921 gggaggaggg ccagggtgaa cagggcgagg aggaggatga ggaggaggaa gaagaggagg
     1981 acgtggctga gaaccgctac tttgaaatgg ggcccccaga cgtggaggag gaggagggag
     2041 gaggccaggg ggaggaagag gaggaggaag aggaggatga agaggccgag gaggagcgcc
     2101 tggctctgga atgggccctg ggcgcggacg aggacttcct gctggagcac atccgcatcc
     2161 tcaaggtgct gtggtgcttc ctgatccatg tgcagggcag tatccgccag ttcgccgcct
     2221 gccttgtgct caccgacttc ggcatcgcag tcttcgagat cccgcaccag gagtctcggg
     2281 gcagcagcca gcacatcctc tcctccctgc gctttgtctt ttgcttcccg catggcgacc
     2341 tcaccgagtt tggcttcctc atgccggagc tgtgtctggt gctcaaggta cggcacagtg
     2401 agaacacgct cttcattatc tcggacgccg ccaacctgca cgagttccac gcggacctgc
     2461 gctcatgctt tgcaccccag cacatggcca tgctgtgtag ccccatcctc tacggcagcc
     2521 acaccagcct gcaggagttc ctgcgccagc tgctcacctt ctacaaggtg gctggcggct
     2581 gccaggagcg cagccagggc tgcttccccg tctacctggt ctacagtgac aagcgcatgg
     2641 tgcagacggc cgccggggac tactcaggca acatcgagtg ggccagctgc acactctgtt
     2701 cagccgtgcg gcgctcctgc tgcgcgccct ctgaggccgt caagtccgcc gccatcccct
     2761 actggctgtt gctcacgccc cagcacctca acgtcatcaa ggccgacttc aaccccatgc
     2821 ccaaccgtgg cacccacaac tgtcgcaacc gcaacagctt caagctcagc cgtgtgccgc
     2881 tctccaccgt gctgctggac cccacacgca gctgtaccca gcctcggggc gcctttgctg
     2941 atggccacgt gctagagctg ctcgtggggt accgctttgt cactgccatc ttcgtgctgc
     3001 cccacgagaa gttccacttc ctgcgcgtct acaaccagct gcgggcctcg ctgcaggacc
     3061 tgaagactgt ggtcatcgcc aagacccccg ggacgggagg cagcccccag ggctcctttg
     3121 cggatggcca gcctgccgag cgcagggcca gcaatgacca gcgtccccag gaggtcccag
     3181 cagaggctct ggccccggcc ccagcggaag tcccagctcc agcccctgca gcagcctcag
     3241 cctcaggccc agcgaagact ccggccccag cagaggcctc aacttcagct ttggtcccag
     3301 aggagacgcc agtggaagct ccagccccac ccccagccga ggcccctgcc cagtacccga
     3361 gtgagcacct catccaggcc acctcggagg agaatcagat cccctcgcac ttgcctgcct
     3421 gcccgtcgct ccggcacgtc gccagcctgc ggggcagcgc catcatcgag ctcttccaca
     3481 gcagcattgc tgaggttgaa aacgaggagc tgaggcacct catgtggtcc tcggtggtgt
     3541 tctaccagac cccagggctg gaggtgactg cctgcgtgct gctctccacc aaggctgtgt
     3601 actttgtgct ccacgacggc ctccgccgct acttctcaga gccactgcag gatttctggc
     3661 atcagaaaaa caccgactac aacaacagcc ctttccacat ctcccagtgc ttcgtgctaa
     3721 agcttagtga cctgcagtca gtcaatgtgg ggcttttcga ccagcatttc cggctgacgg
     3781 gttccacccc gatgcaggtg gtcacgtgct tgacgcggga cagctacctg acgcactgct
     3841 tcctccagca cctcatggtc gtgctgtcct ctctggaacg cacgccctcg ccggagcctg
     3901 ttgacaagga cttctactcc gagtttggga acaagaccac agggaagatg gagaactacg
     3961 agctgatcca ctctagtcgc gtcaagttta cctaccccag tgaggaggag attggggacc
     4021 tgacgttcac tgtggcccaa aagatggctg agccagagaa ggccccagcc ctcagcatcc
     4081 tgctgtacgt gcaggccttc caggtgggca tgccaccccc tgggtgctgc aggggccccc
     4141 tgcgccccaa gacactcctg ctcaccagct ccgagatctt cctcctggat gaggactgtg
     4201 tccactaccc actgcccgag tttgccaaag agccgccgca gagagacagg taccggctgg
     4261 acgatggccg ccgcgtccgg gacctggacc gagtgctcat gggctaccag acctacccgc
     4321 aggccctcac cctcgtcttc gatgacgtgc aaggtcatga cctcatgggc agtgtcaccc
     4381 tggaccactt tggggaggtg ccaggtggcc cggctagagc cagccagggc cgtgaagtcc
     4441 agtggcaggt gtttgtcccc agtgctgaga gcagagagaa gctcatctcg ctgttggctc
     4501 gccagtggga ggccctgtgt ggccgtgagc tgcctgtcga gctcaccggc tagcccaggc
     4561 cacagccagc ctgtcgtgtc cagcctgacg cctactgggg cagggcagca ggcttttgtg
     4621 ttctctaaaa atgttttatc ctccctttgg taccttaatt tgactgtcct cgcagagaat
     4681 gtgaacatgt gtgtgtgttg tgttaattct ttctcatgtt gggagtgaga atgccgggcc
     4741 cctcagggct gtcggtgtgc tgtcagcctc ccacaggtgg tacagccgtg cacaccagtg
     4801 tcgtgtctgc tgttgtggga ccgttgttaa cacgtgacac tgtgggtctg actttctctt
     4861 ctacacgtcc tttcctgaag tgtcgagtcc agtcctttgt tgctgttgct gttgctgttg
     4921 ctgttgctgt tgctgttggc atcttgctgc taatcctgag gctggtagca gaatgcacat
     4981 tggaagctcc caccccatat tgttcttcaa agtggaggtc tcccctgatc cagacaagtg
     5041 ggagagcccg tgggggcagg ggacctggag ctgccagcac caagcgtgat tcctgctgcc
     5101 tgtattctct attccaataa agcagagttt gacaccgtc
//
