LOCUS       NM_001291867            9044 bp    mRNA    linear   PRI 05-OCT-2024
DEFINITION  Homo sapiens NHS actin remodeling regulator (NHS), transcript
            variant 3, mRNA.
ACCESSION   NM_001291867 XM_005274539
VERSION     NM_001291867.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 9044)
  AUTHORS   Clarke,J.M., Alikian,M., Xiao,S., Kasperaviciute,D., Thomas,E.,
            Turbin,I., Olupona,K., Cifra,E., Curetean,E., Ferguson,T.,
            Redhead,J. and Shovlin,C.L.
  CONSRTM   Genomics England Research Consortium
  TITLE     Low grade mosaicism in hereditary haemorrhagic telangiectasia
            identified by bidirectional whole genome sequencing reads through
            the 100,000 Genomes Project clinical diagnostic pipeline
  JOURNAL   J Med Genet 57 (12), 859-862 (2020)
   PUBMED   32303606
  REMARK    GeneRIF: Low grade mosaicism in hereditary haemorrhagic
            telangiectasia identified by bidirectional whole genome sequencing
            reads through the 100,000 Genomes Project clinical diagnostic
            pipeline.
REFERENCE   2  (bases 1 to 9044)
  AUTHORS   Hernandez,V., Pascual-Camps,I., Aparisi,M.J., Martinez-Matilla,M.,
            Martinez,F., Ceron,J.A. and Pedrola,L.
  TITLE     Great clinical variability of Nance Horan syndrome due to
            deleterious NHS mutations in two unrelated Spanish families
  JOURNAL   Ophthalmic Genet 40 (6), 553-557 (2019)
   PUBMED   31755796
  REMARK    GeneRIF: Deleterious NHS mutations are associated with Nance Horan
            syndrome.
REFERENCE   3  (bases 1 to 9044)
  AUTHORS   Ling,C., Sui,R., Yao,F., Wu,Z., Zhang,X. and Zhang,S.
  TITLE     Whole exome sequencing identified a novel truncation mutation in
            the NHS gene associated with Nance-Horan syndrome
  JOURNAL   BMC Med Genet 20 (1), 14 (2019)
   PUBMED   30642278
  REMARK    GeneRIF: Nance-Horan syndrome (NHS) is an X-linked inheritance
            disorder characterized by bilateral congenital cataracts, and
            facial and dental dysmorphism. This disorder is caused by mutations
            in the NHS gene. However, NHS may be difficult to detect in
            individuals with subtle facial dysmorphism and dental abnormalities
            in whom congenital cataracts are the primary clinical
            manifestations.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 9044)
  AUTHORS   Li,H., Yang,L., Sun,Z., Yuan,Z., Wu,S. and Sui,R.
  TITLE     A novel small deletion in the NHS gene associated with Nance-Horan
            syndrome
  JOURNAL   Sci Rep 8 (1), 2398 (2018)
   PUBMED   29402928
  REMARK    GeneRIF: A novel small deletion in the NHS gene is associated with
            Nance-Horan syndrome in a Chinese pedigree.
            Publication Status: Online-Only
REFERENCE   5  (bases 1 to 9044)
  AUTHORS   Brooks,S.P., Coccia,M., Tang,H.R., Kanuga,N., Machesky,L.M.,
            Bailly,M., Cheetham,M.E. and Hardcastle,A.J.
  TITLE     The Nance-Horan syndrome protein encodes a functional WAVE homology
            domain (WHD) and is important for co-ordinating actin remodelling
            and maintaining cell morphology
  JOURNAL   Hum Mol Genet 19 (12), 2421-2432 (2010)
   PUBMED   20332100
  REMARK    GeneRIF: these data identify NHS as a new regulator of actin
            remodelling.
REFERENCE   6  (bases 1 to 9044)
  AUTHORS   Burdon,K.P., McKay,J.D., Sale,M.M., Russell-Eggitt,I.M.,
            Mackey,D.A., Wirth,M.G., Elder,J.E., Nicoll,A., Clarke,M.P.,
            FitzGerald,L.M., Stankovich,J.M., Shaw,M.A., Sharma,S., Gajovic,S.,
            Gruss,P., Ross,S., Thomas,P., Voss,A.K., Thomas,T., Gecz,J. and
            Craig,J.E.
  TITLE     Mutations in a novel gene, NHS, cause the pleiotropic effects of
            Nance-Horan syndrome, including severe congenital cataract, dental
            anomalies, and mental retardation
  JOURNAL   Am J Hum Genet 73 (5), 1120-1130 (2003)
   PUBMED   14564667
  REMARK    GeneRIF: key functions in the regulation of eye, tooth, brain, and
            craniofacial development
REFERENCE   7  (bases 1 to 9044)
  AUTHORS   Toutain,A., Dessay,B., Ronce,N., Ferrante,M.I., Tranchemontagne,J.,
            Newbury-Ecob,R., Wallgren-Pettersson,C., Burn,J., Kaplan,J.,
            Rossi,A., Russo,S., Walpole,I., Hartsfield,J.K., Oyen,N.,
            Nemeth,A., Bitoun,P., Trump,D., Moraine,C. and Franco,B.
  TITLE     Refinement of the NHS locus on chromosome Xp22.13 and analysis of
            five candidate genes
  JOURNAL   Eur J Hum Genet 10 (9), 516-520 (2002)
   PUBMED   12173028
REFERENCE   8  (bases 1 to 9044)
  AUTHORS   Walpole,S.M., Ronce,N., Grayson,C., Dessay,B., Yates,J.R., Trump,D.
            and Toutain,A.
  TITLE     Exclusion of RAI2 as the causative gene for Nance-Horan syndrome
  JOURNAL   Hum Genet 104 (5), 410-411 (1999)
   PUBMED   10394933
REFERENCE   9  (bases 1 to 9044)
  AUTHORS   Bardakjian,T., Weiss,A. and Schneider,A.
  TITLE     Microphthalmia/Anophthalmia/Coloboma Spectrum - RETIRED CHAPTER,
            FOR HISTORICAL REFERENCE ONLY
  JOURNAL   (in) Adam MP, Feldman J, Mirzaa GM, Pagon RA, Wallace SE and
            Amemiya A (Eds.);
            GENEREVIEWS(R);
            (1993)
   PUBMED   20301552
REFERENCE   10 (bases 1 to 9044)
  AUTHORS   Stambolian,D., Lewis,R.A., Buetow,K., Bond,A. and Nussbaum,R.
  TITLE     Nance-Horan syndrome: localization within the region Xp21.1-Xp22.3
            by linkage analysis
  JOURNAL   Am J Hum Genet 47 (1), 13-19 (1990)
   PUBMED   1971992
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL845433.4, AY436752.1,
            BC136415.1 and CR749300.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Jun 2, 2019 this sequence version replaced NM_001291867.1.
            
            Summary: This gene encodes a protein containing four conserved
            nuclear localization signals. The encoded protein functions in eye,
            tooth, craniofacial and brain development, and it can regulate
            actin remodeling and cell morphology. Mutations in this gene have
            been shown to cause Nance-Horan syndrome, and also X-linked
            cataract-40. Alternatively spliced transcript variants encoding
            different isoforms have been described for this gene. [provided by
            RefSeq, May 2014].
            
            Transcript Variant: This variant (3) represents the longest
            transcript and encodes the longest isoform (3).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038194.1845160.1,
                                           SRR14038197.1717746.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968189, SAMEA2144120
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000676302.1/ ENSP00000502262.1
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-220               AL845433.4         32188-32407         c
            221-494             AY436752.1         1-274
            495-5550            BC136415.1         1-5056
            5551-8365           AY436752.1         5268-8082
            8366-9044           CR749300.1         4922-5600
FEATURES             Location/Qualifiers
     source          1..9044
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="X"
                     /map="Xp22.2-p22.13"
     gene            1..9044
                     /gene="NHS"
                     /gene_synonym="CTRCT40; CXN; SCML1"
                     /note="NHS actin remodeling regulator"
                     /db_xref="GeneID:4810"
                     /db_xref="HGNC:HGNC:7820"
                     /db_xref="MIM:300457"
     exon            1..1123
                     /gene="NHS"
                     /gene_synonym="CTRCT40; CXN; SCML1"
                     /inference="alignment:Splign:2.1.0"
     CDS             559..5514
                     /gene="NHS"
                     /gene_synonym="CTRCT40; CXN; SCML1"
                     /note="isoform 3 is encoded by transcript variant 3;
                     congenital cataracts and dental anomalies protein;
                     Nance-Horan syndrome (congenital cataracts and dental
                     anomalies); actin remodeling regulator NHS"
                     /codon_start=1
                     /product="actin remodeling regulator NHS isoform 3"
                     /protein_id="NP_001278796.1"
                     /db_xref="CCDS:CCDS94562.1"
                     /db_xref="GeneID:4810"
                     /db_xref="HGNC:HGNC:7820"
                     /db_xref="MIM:300457"
                     /translation="MPFAKRIVEPQWLCRQRRPAPGPAVDASGGSAEPPPPLQPPGRR
                     DLDEVEAPGPEEPARAVPAPSGLPPPPPPLPAPADQTQPPHGEASVAGEESTAGIPEA
                     APAAGEASSAAAAAAVLLMLDLCAVSNAALARVLRQLSDVARHACSLFQELESDIQLT
                     HRRVWALQGKLGGVQRVLSTLDPKQEAVPVSNLDIESKLSVYYRAPWHQQRNIFLPAT
                     RPPCVEELHRHARQSLQALRREHRSRSDRREQRAAAPLSIAAPPLPAYPPAHSQRRRE
                     FKDRHFLTFNSTRSPSPTECCHMTPWSRKSHPPEDEDTDVMLGQRPKNPIHNIPSTLD
                     KQTNWSKALPLPTPEEKMKQDAQVISSCIIPINVTGVGFDREASIRCSLVHSQSVLQR
                     RRKLRRRKTISGIPRRVQQEIDSDESPVARERNVIVHTNPDPSNTVNRISGTRDSECQ
                     TEDILIAAPSRRRIRAQRGQSIAASLSHSAGNISALADKGDTMFTPAVSSRTRSRSLP
                     REGNRGGDAEPKVGAKPSAYEEGESFVGDHERTPNDFSEAPSSPSAQDHQPTLGLACS
                     QHLHSPQHKLSERGRSRLSRMAADSGSCDISSNSDTFGSPIHCISTAGVLLSSHMDQK
                     DDHQSSSGNWSGSSSTCPSQTSETIPPAASPPLTGSSHCDSELSLNTAPHANEDASVF
                     VTEQYNDHLDKVRGHRANSFTSTVADLLDDPNNSNTSDSEWNYLHHHHDASCRQDFSP
                     ERPKADSLGCPSFTSMATYDSFLEKSPSDKADTSSHFSVDTEGYYTSMHFDCGLKGNK
                     SYVCHYAALGPENGQGVGASPGLPDCAWQDYLDHKRQGRPSISFRKPKAKPTPPKRSS
                     SLRKSDGNADISEKKEPKISSGQHLPHSSREMKLPLDFANTPSRMENANLPTKQEPSW
                     INQSEQGIKEPQLDASDIPPFKDEVAESTHYADLWLLNDLKTNDPYRSLSNSSTATGT
                     TVIECIKSPESSESQTSQSESRATTPSLPSVDNEFKLASPEKLAGLASPSSGYSSQSE
                     TPTSSFPTAFFSGPLSPGGSKRKPKVPERKSSLQQPSLKDGTISLSKDLELPIIPPTH
                     LDLSALHNVLNKPFHHRHPLHVFTHNKQNTVGETLRSNPPPSLAITPTILKSVNLRSI
                     NKSEEVKQKEENNTDLPYLEESTLTTAALSPSKIRPHTANKSVSRQYSTEDTILSFLD
                     SSAVEMGPDKLHLEKNSTFDVKNRCDPETITSAGSSLLDSNVTKDQVRTETEPIPENT
                     PTKNCAFPTEGFQRVSAARPNDLDGKIIQYGPGPDETLEQVQKAPSAGLEEVAQPESV
                     DVITSQSDSPTRATDVSNQFKHQFVMSRHHDKVPGTISYESEITSVNSFPEKCSKQEN
                     IASGISAKSASDNSKAEETQGNVDEASLKESSPSDDSIISPLSEDSQAEAEGVFVSPN
                     KPRTTEDLFAVIHRSKRKVLGRKDSGDMSVRSKSRAPLSSSSSSASSITSPSSNVTTP
                     NSQRSPGLIYRNAKKSNTSNEEFKLLLLKKGSRSDSSYRMSATEILKSPILPKPPGEL
                     TAESPQSTDDAHQGSQGAEALSPLSPCSPRVNAEGFSSKSFATSASARVGRSRAPPAA
                     SSSRYSVRCRLYNTPMQAISEGETENSDGSPHDDRSSQSST"
     exon            1124..1276
                     /gene="NHS"
                     /gene_synonym="CTRCT40; CXN; SCML1"
                     /inference="alignment:Splign:2.1.0"
     exon            1277..1410
                     /gene="NHS"
                     /gene_synonym="CTRCT40; CXN; SCML1"
                     /inference="alignment:Splign:2.1.0"
     exon            1411..1473
                     /gene="NHS"
                     /gene_synonym="CTRCT40; CXN; SCML1"
                     /inference="alignment:Splign:2.1.0"
     exon            1474..1666
                     /gene="NHS"
                     /gene_synonym="CTRCT40; CXN; SCML1"
                     /inference="alignment:Splign:2.1.0"
     exon            1667..1798
                     /gene="NHS"
                     /gene_synonym="CTRCT40; CXN; SCML1"
                     /inference="alignment:Splign:2.1.0"
     exon            1799..4780
                     /gene="NHS"
                     /gene_synonym="CTRCT40; CXN; SCML1"
                     /inference="alignment:Splign:2.1.0"
     exon            4781..4907
                     /gene="NHS"
                     /gene_synonym="CTRCT40; CXN; SCML1"
                     /inference="alignment:Splign:2.1.0"
     exon            4908..9044
                     /gene="NHS"
                     /gene_synonym="CTRCT40; CXN; SCML1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      9023..9028
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NHS"
                     /gene_synonym="CTRCT40; CXN; SCML1"
                     /note="hexamer: AATAAA"
     polyA_site      9044
                     /gene="NHS"
                     /gene_synonym="CTRCT40; CXN; SCML1"
                     /note="major polyA site"
ORIGIN      
        1 agagcgcctt tccgaaacct cctccccgcc cagccaggga gagagatccc gggcgcaatc
       61 gctccccgga gcggccgagg ggcgcgcggg gagaggcctg cgccggggta ctttcaaact
      121 gaggcgcgcg cacacactca cacccaccca cccactcacc cacacacaca cagacacacg
      181 cacgcccatt tatataggcg gcggcgacgg cagtggccgg ggtggcgacg gcgagcacag
      241 aaacgatgga gttgagccag tcgaccctgg ttggaagcaa gtgagaagag accaggcgca
      301 cccctaaatt tctgtgcgga gcgcttgtca tcctccccag ggaacggtgc acccaaagga
      361 ggactggctg gactgatttg ctagggagag ggcggggaag aggaggcaag gtgagcagag
      421 aagccccctg cgtatccgga ctgccagatc gcgcttttgc cggactcctg ccccctccct
      481 gctcaggtgg gcgcgcccgg tctccagctc acaggggcgc ttggaggaga ccagaaagtc
      541 gccgcctggc tgcgcgggat gcctttcgcc aagcggatcg tggagccgca atggctgtgc
      601 aggcagcggc gccctgcgcc cggcccagca gtggacgcga gcggaggcag cgctgagccg
      661 ccgccgccct tgcagccgcc gggccggagg gacctggacg aggtcgaggc gccagggcca
      721 gaggagccag cccgcgccgt ccctgcacct tcagggctgc caccgccgcc gccgccactg
      781 cccgcgccgg ccgaccagac tcagccgccg cacggagagg cgtccgtggc tggcgaggag
      841 agcacggcgg ggatcccgga ggcggcgccc gcagccggcg aggcgtcctc ggcggcggcg
      901 gcggcggccg tgctgctcat gctggaccta tgcgcggtca gcaacgccgc tctggcccgt
      961 gtcctccggc agctctcgga cgtggcccgg cacgcttgca gcctcttcca ggagctcgag
     1021 agcgacatcc agctcaccca ccgccgcgtc tgggcgctgc agggcaagct cggcggcgtg
     1081 cagcgcgtcc tcagcacgct tgaccctaag caggaggcag tgcccgtctc caacctggac
     1141 atagagagta agctgagtgt gtactaccgc gccccgtggc accagcagcg caacatcttc
     1201 ctcccagcca caaggccacc ctgcgtggag gagctgcacc gccacgcccg gcagagcctg
     1261 caagccctgc gcagagaaca ccggagccgg agcgatcgcc gagagcaaag agcagctgcc
     1321 cccctttcca ttgcagctcc tccactgcca gcctaccctc cagctcacag ccagaggagg
     1381 cgtgagttta aggaccgtca ctttttaacg tttaacagca cccgttcgcc ctcccccact
     1441 gaatgttgcc acatgacccc gtggagtaga aagtcccatc ccccagagga tgaagataca
     1501 gatgtcatgt tagggcagag gccgaaaaac ccaatacaca atatcccttc cacactggac
     1561 aagcagacca actggagcaa agcactacct ctcccgacgc cagaggagaa gatgaaacaa
     1621 gatgcccaag tgatttcttc ttgcattatt cccatcaatg ttactggagt tggctttgac
     1681 agagaggcta gtatacgctg ctctctggtt cattcacaat cggtactaca gcggagacga
     1741 aaattgagga ggaggaaaac catctcgggt atccccagaa gagttcaaca agaaatagat
     1801 tctgatgaat caccagtggc cagggaaagg aatgtgattg tgcacacaaa cccagacccc
     1861 tccaacactg tcaataggat atccggaacc agggactctg agtgccaaac cgaggatatt
     1921 ctgattgctg ccccatccag aaggagaatc agagctcaaa ggggtcaaag cattgcagct
     1981 tccctttctc attctgctgg caacatttct gccctagcag acaaaggtga caccatgttt
     2041 actcctgcag tgagcagccg cacaagatct cggagccttc cccgggaagg taatagaggt
     2101 ggggatgctg agcccaaagt tggcgctaaa ccctcagcat atgaagaggg agagtctttt
     2161 gtgggtgacc atgaaagaac ccctaatgat ttcagtgagg ctccaagcag cccgagtgcc
     2221 caggaccacc agcctacttt gggcctggcc tgctctcaac atcttcacag cccccagcac
     2281 aaattaagtg agaggggaag gtcacgtctg tcccgaatgg ctgctgactc tggcagctgt
     2341 gacatctcct ccaactcaga cacgtttggg agccccatcc actgcatctc cacggctggc
     2401 gtcctcctta gcagccacat ggaccagaaa gatgaccacc agtcatccag tggcaactgg
     2461 agtgggagca gctccacgtg cccctcgcag acctcagaaa ccatccctcc tgcagcttct
     2521 cctccactca ctggctcttc acactgtgac tcggagttgt cactaaacac agcccctcat
     2581 gccaatgagg atgccagtgt tttcgtgaca gagcaataca atgaccactt ggataaagtg
     2641 agaggccatc gggcaaactc ctttacctcc actgttgcag acctgctgga tgatcccaac
     2701 aacagcaaca caagtgacag tgagtggaat tacctacacc accaccatga tgcctcctgc
     2761 cgccaggatt ttagtcctga gcgtcccaag gcagacagcc tgggctgccc aagcttcaca
     2821 agcatggcca cttatgacag ctttctggaa aagtctccat cagacaaagc ggacactagc
     2881 tctcactttt cagtagacac ggaaggatac tatacctcca tgcactttga ctgtggtctc
     2941 aaaggtaata agagctatgt ctgtcactat gcagccctgg gcccagagaa tggccagggt
     3001 gtaggggctt cccctggtct tccagattgt gcctggcagg actacttaga ccacaagagg
     3061 cagggaagac caagcatctc tttcaggaaa ccaaaggcaa agccgacccc acctaaacgt
     3121 agctcatcat tgaggaagtc tgatggaaac gcagatattt ctgagaagaa agaaccaaag
     3181 ataagcagtg gtcagcacct gcctcacagt tccagggaaa tgaagctgcc tcttgatttc
     3241 gccaacacgc cttctcgaat ggaaaacgcc aatcttccca ccaagcagga accttcttgg
     3301 ataaaccaga gtgaacaagg cattaaggaa cctcagttag atgcttcgga tattccacca
     3361 ttcaaagatg aagttgccga atccacacac tatgcagacc tctggctcct aaatgacttg
     3421 aaaacaaatg atccttatag atctctatct aattcaagca ccgctacggg taccacagtc
     3481 attgaatgca tcaaatctcc agagagctct gaatcccaaa catcacaatc agaatcaaga
     3541 gccaccaccc catctcttcc ttctgttgac aatgagttta aactggcttc accagaaaag
     3601 ctggctggct tggcatctcc atcaagtggc tattcaagcc agtctgaaac gccaacatcc
     3661 tctttcccta cagctttctt ttcaggtcca ttgtctcccg gaggtagcaa aagaaaacct
     3721 aaagtcccag aaagaaaatc ctcactacag caaccctctt taaaagatgg aactatatca
     3781 ctgagtaaag accttgaact tccaattata cctcctaccc atcttgatct aagtgctctt
     3841 cataatgtct tgaacaaacc attccaccac cgtcatccac tgcatgtttt tactcataat
     3901 aagcagaaca cagtaggaga aacactgagg tcgaatcctc caccgtccct tgcaattaca
     3961 ccaacgatcc tgaaatctgt taaccttagg tccatcaaca agtctgaaga agttaagcaa
     4021 aaagaagaaa acaatacaga tctcccttat ttagaggaaa gcacactcac aacggctgcc
     4081 ttgtctccaa gtaagattag gccgcataca gcaaataaat cagtatctcg tcaatactcc
     4141 actgaagaca ccatactgtc ctttttagac tcttctgcag ttgagatggg accagataaa
     4201 ctacatttag aaaaaaactc tacttttgat gtgaagaatc gctgcgatcc agaaaccata
     4261 acatcagctg gtagcagtct tctagattca aatgtcacaa aagaccaagt gcgtacagag
     4321 actgagccta ttccagaaaa cacgccaacc aaaaactgtg cttttcccac agaaggattt
     4381 cagagggtct ctgctgcccg cccaaatgat ttggatggta aaataataca atatggacct
     4441 ggtccagacg aaactctaga acaggtacag aaggcaccct ctgcaggtct ggaggaagtt
     4501 gcacaacctg aatctgtgga tgtaatcaca tctcagtcag actcaccaac tagagcaaca
     4561 gatgtaagca atcaatttaa gcatcaattt gttatgagcc gccaccatga caaagtgcct
     4621 ggtactatca gctatgaatc ggagataaca tctgtaaatt cattccctga aaaatgttcc
     4681 aagcaggaaa atattgcttc aggtatttca gccaaaagtg cctctgataa cagcaaagca
     4741 gaggagaccc aaggaaatgt ggatgaggct tcattgaaag aatcatcacc gagtgatgac
     4801 tccatcattt caccacttag tgaagactcc caagctgaag cagagggtgt gttcgtgtct
     4861 ccaaacaaac ctcgaacaac tgaggattta tttgcagtca ttcacagatc caagaggaaa
     4921 gtacttggaa gaaaagattc cggggacatg tctgttcgaa gcaaatcgag agctcccctc
     4981 agcagtagca gcagcagcgc cagttccatc acttcaccca gcagtaatgt gacaaccccc
     5041 aacagccaga ggtctcctgg tctcatatac cgaaatgcca aaaagtccaa cacatccaat
     5101 gaagagttta agctgttact gctcaagaaa ggcagtcgct cagattctag ttaccgcatg
     5161 tctgccactg agatcctgaa gagccccata ctgcccaaac ctcctgggga gctcacagca
     5221 gagtcccctc agagcaccga tgatgcccat caggggtcac aaggggctga ggcattgtcc
     5281 ccactctctc catgctcccc acgagttaat gcagaaggct tttcctcgaa gagctttgcc
     5341 acctcagcat cagcaagggt tggacgttct cgggcccctc ctgcagccag cagcagccgc
     5401 tacagtgtcc gctgccggct gtacaatacg cccatgcagg caatctccga gggagagacg
     5461 gaaaattctg acgggagccc acatgacgac cgttcctccc agagttcaac atagactgcc
     5521 tgtaccaggc tgcctggcaa aggccaaaac ccttactcac atgaggatgg aggaacagaa
     5581 cagaggactt gggaaaagtc tcaacttgat ggggtagcat cactgctaag caatgaatga
     5641 atttctaaat acagtatgtg ctgggtaaac agaaagtggt ttagacattc ttgattagtt
     5701 tccatatttt aagtagctgc agtctttcat gtttttcttt agcatagttt gatttacgca
     5761 atctccttcc ttgtgaaaat agaggataca aaattgtcta attttcatga cacctagaaa
     5821 acgttttccc agagctgcct attcagaaac taaagccctc actgtcatta ttctttaaga
     5881 agatgaaatt actctggatg tttggtattt ttttacatta aaacaaacta aagcaaaatt
     5941 tagaagaaag aactacacat atgtaaatac catatttttg ataaaaatgt gtaaatagat
     6001 ttatcaatga tgagtggaca tgtggtcaat tatctactgc acaccaactg tttatagaat
     6061 acacaaaaat aaaatgtaat aactgtattc tgtgaatacc attttcatat caaatgccat
     6121 atttaaatgt ccaccaatat gatttctgag tggtaaacct caaatatgaa ttttaaactg
     6181 gtgaactaaa ggaaatcttg aggtcatata ctgaaatatg aataaattaa aataatgaat
     6241 tcagatctaa tcatttttat gacaaattgc agcaccaaac aaactaatag caacccatgg
     6301 ctgtgatttg ttgtgtggta atttggacag aatgaaaagc atgcttttga tttttttttt
     6361 aatcagtgag agaagtcatc attctcaaca caaagccctg aacaacagca tttgacagtg
     6421 tcctttagat tttaattttt tcaatggatt gctttaaaga gaatgagtag ggaaaatagt
     6481 agttaatttt aggttatgtt ttatattagg ctactgggga cataaacggt cataactaat
     6541 gactgtaatc attaaactcc ttaaacagtt tagaaattag ctccaggttc ttaaactaac
     6601 aaaaataaaa cctaagcatg ccacagagct attgattaat cagtaagtac ctgtaatgag
     6661 ttttcagtga agctttctct ctgtgctgat gagattcatg ctacctaaaa attaacagaa
     6721 atgacactgt gaacaatgta gttggaaaat aggtatgtgt atatgcatta tttatactca
     6781 ttgttaaact gggaatgggg aacagctctg gttcacaata ctacatacaa ctgagttttt
     6841 gtctggtttt actatagtgt ctgcttgatg gcaaaagggg agggtggagg gtgggagagg
     6901 aaatgtcagg gcatgtgctc tgataaaata aaggcaggga aacaagctga attacttgaa
     6961 attacttgaa ttttcttgtc ttaaaactga aaaaaaaatg tagttacacg ttaaaatctg
     7021 caaatggttt ttacacctct gattttaaca tgaacttata ctaatgtttg gaatcttatg
     7081 tcagaaatat aagcagctat gtacttagaa taggttttga atgggagagg tagaacagag
     7141 agagaattaa gaagggatct gacttataaa agactagaat gtgattagag tgatagaaca
     7201 taccaatgtt accaagaaat tgacaagctg ctggctttaa gcttatgcaa gtggtagttg
     7261 ggaaagtagg aggtgtggaa gagggtttgc attttggatt aattcatgca aaatgaagga
     7321 ggaagcctgg tctaagaaga tactgtcttt caatagaaat gatttctaaa ctgctacaga
     7381 ttaagaatag ataatctgat tgctgttgtt ttgtttgttt ggaaagaaaa aaaatgtctg
     7441 gcttcttcta ctatttgttt tcactaccaa actgtgttac taaatttctt gtcatccttg
     7501 tatgtaaaat gggtgctggg ggtggagggg tataagagga gggagagtca gagagagtgt
     7561 gtatgggtgt gtgtgagtgt gagtgtgtgt gtacgcacac acactgggga tagataagct
     7621 acctggtaaa gggtttgaac atttacaaaa tgtcacactt tttcttaaaa gaaaaatatt
     7681 ttggggtttg aataaaatgg accaccattt ctcattggaa cccattaatt aagaaaacca
     7741 gcatggtttg acaccacatg ggaacatgaa taaatctgtc tcatgatttg aaaagtacac
     7801 cttgcaaagt tttaaaaata aagtttttag gcaaatgcga taagaaaccg tcatttccag
     7861 tcacagtagg cgatctttcg taatttcata aaagcagttc gtttgcagta tggcttttgt
     7921 gtagttaggg gttttttaag tgtgaagaaa ggtatgtggg ctttaaaagt gattctaaat
     7981 cttgaataga aaacacatga attggcttta ataaaaatgt caccttttta ttattgacat
     8041 taagtaatgg taccatatat tttttaaaat acatattgac ttgaattgtg aggcaataag
     8101 ataccttcta tatgtaatga tcacagaact aacccttata atatagtttt acttattttg
     8161 tttactctaa aaatctatag cagagtttct ctattttata tttcatagat ttaaaaaacc
     8221 caaataaaga tggatttaaa attcactgtg gaaaagagta ttgagttaca aactgagaag
     8281 aaaaagcagg aagttcccta ataatctgga atattctaca tggcttgatt acatagcaaa
     8341 caagaccaag atttagggct gtctttaaca tcactgccgt cttgaagcag ggtacacacg
     8401 ttaggtattg tgaagaacat caatccgaac ttttcttctg ttgtttgcac tgatgcgatt
     8461 ttttgttctt ttatgtttat acaacgtatc tatgttgtgt aggggaaatt ttttcatttt
     8521 ttaaaaattc tactttggtt ttgttgttgt tttgatttgt ttttggctaa gtagaggaca
     8581 tggactagtt gtagcaaaca gaacatgctg tttgctcttg ccaaaggtca gtgagtgagt
     8641 gcatttgctg cagtggtggc acgtagagaa ctagatggta ataaagattt aaaactatga
     8701 agaaggttac agtgtaacta ttttggtact agaaccagtt catgctggcc gaaacgacaa
     8761 tggtttatgg acttgcatcc attttgtatt tttgtaatat ttgtaaatat gaacttttta
     8821 aattgctgca aagatggttt cttttgtaag atgttttcaa cgtttacatt caatccaagc
     8881 ctttgtatat tttagagctg tgcaacactt taagtcttgt atttattttt agtaaaaatg
     8941 gtgacagttt cattgtgaac ccctctcaaa aaaaatgtat cagaaaagtt tgattaccta
     9001 gaaagtgtgt atagaaactg caaataaacg tgactgcaat taaa
//
