LOCUS       NM_198514              11051 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens NHL repeat containing 2 (NHLRC2), mRNA.
ACCESSION   NM_198514 NM_017687 XM_350803
VERSION     NM_198514.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 11051)
  AUTHORS   Sczakiel,H.L., Zhao,M., Wollert-Wulf,B., Danyel,M., Ehmke,N.,
            Stoltenburg,C., Damseh,N., Al-Ashhab,M., Balci,T.B., Osmond,M.,
            Andrade,A., Schallner,J., Porrmann,J., McDonald,K., Liao,M.,
            Oppermann,H., Platzer,K., Dierksen,N., Mojarrad,M., Eslahi,A.,
            Bakaeean,B., Calame,D.G., Lupski,J.R., Firoozfar,Z.,
            Seyedhassani,S.M., Mohammadi,S.A., Anwaar,N., Rahman,F., Seelow,D.,
            Janz,M., Horn,D., Maroofian,R. and Boschann,F.
  TITLE     Broadening the phenotypic and molecular spectrum of FINCA syndrome:
            Biallelic NHLRC2 variants in 15 novel individuals
  JOURNAL   Eur J Hum Genet 31 (8), 905-917 (2023)
   PUBMED   37188825
  REMARK    GeneRIF: Broadening the phenotypic and molecular spectrum of FINCA
            syndrome: Biallelic NHLRC2 variants in 15 novel individuals.
REFERENCE   2  (bases 1 to 11051)
  AUTHORS   Kreus,M., Lehtonen,S., Hinttala,R., Salonen,J., Porvari,K. and
            Kaarteenaho,R.
  TITLE     NHLRC2 expression is increased in idiopathic pulmonary fibrosis
  JOURNAL   Respir Res 23 (1), 206 (2022)
   PUBMED   35964085
  REMARK    GeneRIF: NHLRC2 expression is increased in idiopathic pulmonary
            fibrosis.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 11051)
  AUTHORS   Brodsky,N.N., Boyarchuk,O., Kovalchuk,T., Hariyan,T., Rice,A.,
            Ji,W., Khokha,M., Lakhani,S. and Lucas,C.L.
  TITLE     Novel compound heterozygous variants in NHLRC2 in a patient with
            FINCA syndrome
  JOURNAL   J Hum Genet 65 (10), 911-915 (2020)
   PUBMED   32435055
  REMARK    GeneRIF: Novel compound heterozygous variants in NHLRC2 in a
            patient with FINCA syndrome.
REFERENCE   4  (bases 1 to 11051)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 11051)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 11051)
  AUTHORS   Paakkola,T., Salokas,K., Miinalainen,I., Lehtonen,S., Manninen,A.,
            Kaakinen,M., Ruddock,L.W., Varjosalo,M., Kaarteenaho,R., Uusimaa,J.
            and Hinttala,R.
  TITLE     Biallelic mutations in human NHLRC2 enhance myofibroblast
            differentiation in FINCA disease
  JOURNAL   Hum Mol Genet 27 (24), 4288-4302 (2018)
   PUBMED   30239752
  REMARK    GeneRIF: We conclude that compound heterozygous p.Asp148Tyr and
            p.Arg201GlyfsTer6 mutations in NHLRC2 lead to severe tissue
            fibrosis in humans by enhancing the differentiation of fibroblasts
            to myofibroblasts.
REFERENCE   7  (bases 1 to 11051)
  AUTHORS   Biterova,E., Ignatyev,A., Uusimaa,J., Hinttala,R. and Ruddock,L.W.
  TITLE     Structural analysis of human NHLRC2, mutations of which are
            associated with FINCA disease
  JOURNAL   PLoS One 13 (8), e0202391 (2018)
   PUBMED   30138417
  REMARK    GeneRIF: Bioinformatics analysis discovers homologs across a range
            of eukaryotic and prokaryotic species and conserved residues map
            mostly to the adjacent surfaces of the Trx-like and beta-propeller
            domains that form the cleft, suggesting both that this forms the
            potential functional site of NHLRC2 and that the function is
            conserved across species.
            Publication Status: Online-Only
REFERENCE   8  (bases 1 to 11051)
  AUTHORS   Uusimaa,J., Kaarteenaho,R., Paakkola,T., Tuominen,H.,
            Karjalainen,M.K., Nadaf,J., Varilo,T., Uusi-Makela,M.,
            Suo-Palosaari,M., Pietila,I., Hiltunen,A.E., Ruddock,L., Alanen,H.,
            Biterova,E., Miinalainen,I., Salminen,A., Soininen,R., Manninen,A.,
            Sormunen,R., Kaakinen,M., Vuolteenaho,R., Herva,R., Vieira,P.,
            Dunder,T., Kokkonen,H., Moilanen,J.S., Rantala,H., Nogee,L.M.,
            Majewski,J., Ramet,M., Hallman,M. and Hinttala,R.
  TITLE     NHLRC2 variants identified in patients with fibrosis,
            neurodegeneration, and cerebral angiomatosis (FINCA):
            characterisation of a novel cerebropulmonary disease
  JOURNAL   Acta Neuropathol 135 (5), 727-742 (2018)
   PUBMED   29423877
  REMARK    GeneRIF: Study identified a novel cerebropulmonary and multi-organ
            disease characterised by a unique combination of tissue fibrosis,
            neurodegeneration and cerebral angiomatosis, and identified
            identical compound heterozygous NHLRC2 variants in three affected
            children from two unrelated families. Also created a KO mouse model
            and zebrafish morphants to investigate the consequences of absent
            or decreased Nhlrc2 expression.
REFERENCE   9  (bases 1 to 11051)
  AUTHORS   Nishi,K., Iwaihara,Y., Tsunoda,T., Doi,K., Sakata,T., Shirasawa,S.
            and Ishikura,S.
  TITLE     ROS-induced cleavage of NHLRC2 by caspase-8 leads to apoptotic cell
            death in the HCT116 human colon cancer cell line
  JOURNAL   Cell Death Dis 8 (12), 3218 (2017)
   PUBMED   29242562
  REMARK    GeneRIF: Reactive oxygen species-induced cleavage of NHLRC2 by
            caspase-8 leads to apoptotic cell death in the HCT116 human colon
            cancer cells.
            Publication Status: Online-Only
REFERENCE   10 (bases 1 to 11051)
  AUTHORS   Grupe,A., Li,Y., Rowland,C., Nowotny,P., Hinrichs,A.L., Smemo,S.,
            Kauwe,J.S., Maxwell,T.J., Cherny,S., Doil,L., Tacey,K., van
            Luchene,R., Myers,A., Wavrant-De Vrieze,F., Kaleem,M.,
            Hollingworth,P., Jehu,L., Foy,C., Archer,N., Hamilton,G.,
            Holmans,P., Morris,C.M., Catanese,J., Sninsky,J., White,T.J.,
            Powell,J., Hardy,J., O'Donovan,M., Lovestone,S., Jones,L.,
            Morris,J.C., Thal,L., Owen,M., Williams,J. and Goate,A.
  TITLE     A scan of chromosome 10 identifies a novel locus showing strong
            association with late-onset Alzheimer disease
  JOURNAL   Am J Hum Genet 78 (1), 78-88 (2006)
   PUBMED   16385451
  REMARK    GeneRIF: Observational study of gene-disease association. (HuGE
            Navigator)
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            AK090631.1, AK098487.1, AL832090.1, BX538287.1 and AL162407.14.
            
            On Nov 22, 2018 this sequence version replaced NM_198514.3.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AK090631.1, SRR1660803.237092.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000369301.3/ ENSP00000358307.3
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2079              AK090631.1         1-2079
            2080-2088           AK098487.1         1223-1231
            2089-2550           AK090631.1         2089-2550
            2551-5441           AL832090.1         551-3441
            5442-6363           BX538287.1         4265-5186
            6364-11051          AL162407.14        18504-23191
FEATURES             Location/Qualifiers
     source          1..11051
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="10"
                     /map="10q25.3"
     gene            1..11051
                     /gene="NHLRC2"
                     /gene_synonym="FINCA"
                     /note="NHL repeat containing 2"
                     /db_xref="GeneID:374354"
                     /db_xref="HGNC:HGNC:24731"
                     /db_xref="MIM:618277"
     exon            1..390
                     /gene="NHLRC2"
                     /gene_synonym="FINCA"
                     /inference="alignment:Splign:2.1.0"
     CDS             213..2393
                     /gene="NHLRC2"
                     /gene_synonym="FINCA"
                     /note="1200003G01Rik; novel NHL repeat domain containing
                     protein"
                     /codon_start=1
                     /product="NHL repeat-containing protein 2"
                     /protein_id="NP_940916.2"
                     /db_xref="CCDS:CCDS7585.1"
                     /db_xref="GeneID:374354"
                     /db_xref="HGNC:HGNC:24731"
                     /db_xref="MIM:618277"
                     /translation="MAAPGGRGRSLSGLLPAQTSLEYALLDAVTQQEKDSLVYQYLQK
                     VDGWEQDLSVPEFPEGLEWLNTEEPISVYKDLCGKIVVLDFFTYCCINCIHLLPDLHA
                     LEHTYSDKDGLLIIGVHSAKFPNEKVLDNIKSAVLRYNITHPMVNDADASLWQELEVS
                     CWPTLVILGPRGNMLFSLIGEGHKDKLFLYTSIALKYYKDRGQIRDNKIGIKLYKDSL
                     PPSPLLFPGKVTVDQVTDRLVIADTGHHRILVVWKNGQIQYSIGGPNPGRKDGIFSES
                     TFNSPQGVAIMNNIIYVADTENHLIRKIDLEAEKVSTVAGIGIQGTDKEGGAKGEQQP
                     ISSPWDVVFGTSGSEVQRGDILWIAMAGTHQIWALLLDSGKLPKKNELTKGTCLRFAG
                     SGNEENRNNAYPHKAGFAQPSGLSLASEDPWSCLFVADSESSTVRTVSLKDGAVKHLV
                     GGERDPMNLFAFGDVDGVGINAKLQHPLGVTWDKKRNLLYVADSYNHKIKVVDPKTKN
                     CTTLAGTGDTNNVTSSSFTESTFNEPGGLCIGENGELLYVADTNNHQIKVMDLETKMV
                     SVLPIFRSENAVVDGPFLVEKQKTLPKLPKSAPSIRLSPVTACAGQTLQFKLRLDLPS
                     GSKLTEGVSSCWFLTAEGNEWLLQGQIAAGDIENISSQPTISLQIPDDCLSLEAIVSV
                     SVFLYYCSADSSACMMKAILFSQPLQITDTQQGCIAPVELRYVF"
     exon            391..543
                     /gene="NHLRC2"
                     /gene_synonym="FINCA"
                     /inference="alignment:Splign:2.1.0"
     exon            544..999
                     /gene="NHLRC2"
                     /gene_synonym="FINCA"
                     /inference="alignment:Splign:2.1.0"
     exon            1000..1121
                     /gene="NHLRC2"
                     /gene_synonym="FINCA"
                     /inference="alignment:Splign:2.1.0"
     exon            1122..1251
                     /gene="NHLRC2"
                     /gene_synonym="FINCA"
                     /inference="alignment:Splign:2.1.0"
     exon            1252..1351
                     /gene="NHLRC2"
                     /gene_synonym="FINCA"
                     /inference="alignment:Splign:2.1.0"
     exon            1352..1583
                     /gene="NHLRC2"
                     /gene_synonym="FINCA"
                     /inference="alignment:Splign:2.1.0"
     exon            1584..1706
                     /gene="NHLRC2"
                     /gene_synonym="FINCA"
                     /inference="alignment:Splign:2.1.0"
     exon            1707..1916
                     /gene="NHLRC2"
                     /gene_synonym="FINCA"
                     /inference="alignment:Splign:2.1.0"
     exon            1917..2136
                     /gene="NHLRC2"
                     /gene_synonym="FINCA"
                     /inference="alignment:Splign:2.1.0"
     exon            2137..11051
                     /gene="NHLRC2"
                     /gene_synonym="FINCA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      11030..11035
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NHLRC2"
                     /gene_synonym="FINCA"
                     /note="hexamer: ATTAAA"
     polyA_site      11051
                     /gene="NHLRC2"
                     /gene_synonym="FINCA"
                     /note="major polyA site"
ORIGIN      
        1 agtttaatta cgtccccggg aactgcgccg atttggactt ttggcacttg gacctatgct
       61 ttaaaaagaa aaaagtgtca ttggcgtgga gtggggctag tggagggtga ggtgaatgcg
      121 ccgtttggaa accacaggac agtgaacgtt tcgtctctcc cagcgagact ctcccgcggg
      181 cccggcggcc gcatcgggag cccggcggaa acatggcggc gcccggaggc cggggccgca
      241 gcctctccgg cctgctcccc gcgcagacct cgctagagta cgccctgctc gacgccgtta
      301 cccagcagga gaaggacagc ctggtctacc agtatctgca gaaggtggac ggctgggagc
      361 aggacttgtc agtacccgag tttccggaag gattagaatg gctgaacaca gaagaaccta
      421 tttctgtcta caaggatcta tgtggaaaaa tagtcgtcct tgatttcttc acctactgct
      481 gcataaactg tattcaccta ttgcctgatc tccatgcatt agaacacaca tactctgata
      541 aagatggtct tcttattatt ggtgttcact cggctaagtt tccaaatgaa aaagtcctgg
      601 ataacattaa gagtgctgtt cttcgataca acatcaccca ccctatggtt aatgatgcag
      661 atgccagcct ttggcaagaa ctagaagttt cctgctggcc aactctagtc atacttggac
      721 ctcgtggaaa catgttgttt tctttgattg gagagggaca caaagataaa ttatttttat
      781 atacttcaat tgctttaaag tattacaaag acagggggca gatcagagat aataaaattg
      841 gaataaaact ctataaagat tctttgccac cttcaccatt gctatttcct ggcaaagtaa
      901 cagtagacca agttactgat agattggtaa tagcagacac tggacatcat agaattttgg
      961 tcgtttggaa gaatggacaa attcaatata gcattggagg acccaaccct ggaagaaaag
     1021 atggaatatt ttcagaatca acttttaatt ctccacaggg tgtagccata atgaataata
     1081 tcatatatgt ggcagacact gaaaaccacc ttataagaaa gattgaccta gaagctgaga
     1141 aggtgagcac tgtagctggt attggaattc aaggtacaga taaagaaggt ggagcaaaag
     1201 gagaacaaca acccattagt tccccttggg atgtagtttt tggaacatca ggttcagagg
     1261 tccaaagagg tgacatttta tggatagcca tggcagggac tcatcagata tgggcactcc
     1321 tgctggactc tggcaaactg ccaaagaaaa atgagttaac aaaaggaacc tgccttaggt
     1381 ttgctggaag tggaaatgaa gagaatcgaa acaatgccta tcctcacaag gcaggttttg
     1441 cccaaccttc aggcctttcc ttggcctctg aagatccctg gagctgcttg tttgtagcag
     1501 atagtgagag cagtacagtg agaaccgttt cactgaaaga tggagcagtg aagcacctcg
     1561 taggaggaga aagagacccc atgaatttat ttgcttttgg tgatgttgat ggagtaggaa
     1621 tcaatgcaaa gcttcaacac ccccttggag taacatggga caaaaaaagg aatttacttt
     1681 atgttgcaga ctcctacaat cacaagatta aagttgtgga tccaaaaaca aaaaactgta
     1741 caacattagc aggaactgga gacacaaata atgttaccag ttccagtttt acagagtcaa
     1801 cttttaatga accaggaggc ttgtgtattg gagagaatgg agaattatta tatgtagcag
     1861 acaccaataa tcatcaaatt aaagtgatgg atttagaaac taaaatggta tctgtgctcc
     1921 ccatcttcag atctgaaaat gctgtggtag atggcccgtt cctagtagaa aaacagaaga
     1981 cattacccaa actacctaaa tctgctccaa gcattaggct ttcccccgtg actgcgtgtg
     2041 ctggccagac tcttcagttc aaactcagat tagacctccc atcaggatca aagctaactg
     2101 aaggagtatc cagttgctgg tttctaacag ctgaaggcaa tgaatggcta cttcaaggac
     2161 agatagcagc tggagatata gagaacattt ccagtcaacc aacaatttca ctacaaattc
     2221 ctgatgattg cttatcactt gaagccattg tatctgtcag tgtgtttctt tattactgta
     2281 gtgcagacag cagtgcttgt atgatgaagg caattttgtt cagtcagcct ttacaaataa
     2341 cggatacaca gcaaggttgc atagctccag tagagctcag gtatgtattt tagccagcca
     2401 gctagctagc aacccattgc caccacctac tgtctcccat cctgactatc actgtaattt
     2461 aaggaaagaa aacttcagtt ctgcctctgg ataccaagat gcccattgct cagttcagac
     2521 aactgatatt aaaataaagc tatgctcctt acttacttct tttattataa acaaattcct
     2581 ttgctttggc tgatactagc tgagtcattg atcatcattg gtaccatgat attgtaatct
     2641 atgctgctat ttggcacaag actgaagttc acactacagt agagaatact ataagataat
     2701 ttgcaataaa tactgataat aataatacca gatattttaa ctaacttttt ctacctttat
     2761 taatagcaat cagcacactt gaatgtgtaa atttcacagt aactttaggc agaacttaag
     2821 ctccaggcca catttgtata agaacaccaa gtattcaagg cataaagtct gttgtaagcc
     2881 aaaaaaaagt cttttcatca ctgtagaagc ttaaggagta catcagtggt aaatacgatc
     2941 tttattctct tctgtggttt tctcagttga gatattttta aaagagagtt ttgatctatt
     3001 tttattataa attatttgtt atttaacata ccattatgaa tgccttaaaa ctttgtacag
     3061 ataaagttgg acatagaatc tctaaggact gactccttta actgtttttg agtgactaca
     3121 ttaccagata atgaatgagt ccccagaaat attatgactt tcaaggaaaa agggacttta
     3181 ggacacatta cttagtgtct gtgataaata atttattgtg acagctaaat ctgatggtta
     3241 catatcttgt tttggaaatg tacatttcac ttgtaagcat taaatgcaga tttgttgagt
     3301 ttaggttttg aggttttttt cgttcccata ttcagaattt aaattatgat catttattgt
     3361 gatgttttta aaaatcactt ttaagcctta aaagtgaaat tttcagtgtg aggtgctttc
     3421 atgctattaa tagatacagt atatcctaat atattagtct tcaaaatatt aaggccgaaa
     3481 cagtgattta atgatactat tttttaattt ttgttgtttt ttatccctaa aaaaagatat
     3541 tggaaaggtt ttttttctat tgtcataatt tataactact taaaagccta atcttgtttt
     3601 ccttaagacc tattcaacat gtttgtgatt taatcagctt acaagggttt aaaaacatca
     3661 ggtgaaccat gtttatttat tcagtaaata tttatagaca atatggcata atatgcttcc
     3721 cttccctgta aaaacagtgc taaatttgaa gtgtaatctt ttttaaacat cattgtctat
     3781 ttttaatttt gtttgaaatg tagtattagt ttgttttatt cataaggtta gctgaatctc
     3841 tgaagtaagg atgtataaat catgacattg atttaattag tcatctactg aagctacttt
     3901 taaagagaaa tatagatata aaattaaaag gaatcctgtt taaaattata tgtaattacc
     3961 tcataaacct tttctctcca caagcaatca aaaaaatgaa atgttctgta ataaaagggt
     4021 gtataacata ctctctttta tgagaatagg ccttagttat tttattttat ttttgagacg
     4081 tagtctggct ctgtagccca ggctgggagg gcagtggcgc gatcttggct cactgcaacc
     4141 tctacctcct gggttcaaga gattttcctg cctcagcctc ccaagtagct gggattacag
     4201 gcgtacacca ccatgcccag ctaatttttt tgtgttttta gtagagacag ggtttcacca
     4261 tgtcagtcag gctggtccca aactcctggc ctcaggtgat ccgcctgcct ctgtctccca
     4321 aagtgctggg attacaggtg tgagccacct tgccctactg gccttagtta atttttaatc
     4381 tcatttgact tattatgttt ctcatttcta gatggacagg atgagaatat tttggcccaa
     4441 tcattttctg tttaaggact taaacaattt cacactctaa atgaagtata tacaatttta
     4501 atttactaat ttagaaatat ctgagtttat ttttcaatat aatatacaaa cacagtgttt
     4561 gttatacatg ttcatgtaca gtgaaaatgc aaaggcattt tttagactct attccccttc
     4621 atttgtctag acagttttag gttagcctta gcttatactt ttcttgactt tcaaaataca
     4681 gttttattga acattcttat tcttacccac tgccagtctg cattgttttt tacattaaaa
     4741 gtattattta tagtagacta atatgatttt gaataaaatc acacttaagc aatatagata
     4801 ggttcctaga atttatgacg aggataaacc tctcacataa gaggaagcac tagaaagttt
     4861 atttttaaaa cttgttgatc catattctat aaggactaat gcatattttt tatcttttgc
     4921 ttttcttgtt ggataaatta ttatgtccag tgatagttta aaaagaacaa gattttagtg
     4981 taagtcacct cacttgaaat gccagtaatg tggtattaca cacattttat ataatacctg
     5041 gaatctttgc cctaaattat tctgctggtt tgtcgctatt tttttcctct cctctttatt
     5101 atttcacaaa tggtagaatt tcttgatatg attcttgtaa agagtttagc ttatcttgaa
     5161 cattctgtct taagcaaata tttaacaaga gaaaactttg tagtttaatc acccataagt
     5221 gtaactttag caaaattcaa tgactatgaa aaataatttt tttcttaact gttctaataa
     5281 tatttagtta taatattatc aaaccttcag aaagttataa atcctaacag ccagaaaaaa
     5341 aaatttacta tgtgtatatt tatataggtg tgtgtataca caaagattaa gaattattca
     5401 tgattctttt atggccaata tacttttgag cttgtataac tgagataagt tactctttgc
     5461 ttgtttttct gtagttggaa aatttaggaa gttattaaaa atacatatct ctataatgta
     5521 atagttttct gcctgcacaa agtttactat taatttatgt ctaccagtta aaaatacgat
     5581 agttagattt ggcatcacgt ttaaccacaa tcatcaaaat ctacttaaat tctatagtta
     5641 acagttacat aagaatatat gcagagggaa aatgtcaata gttcttgtga atgaaggaat
     5701 tatggatgtt tttattttaa ttctactttg ctgttttttt tttcttttca gtgagcaatc
     5761 tgttattttt aaaatcacgg tggggggaaa cccataaagt ttaaagagtt aaagttaact
     5821 gaatcgtaag cacttatata ataacttttt aaaagtcatt tttattacca gagctcagaa
     5881 cattgtttgg agtcctttga tctaccggtt ctcttaagta cgtgatttcg tgaagatagc
     5941 aatggttatg gcttttttgt tctgttattt ttattgtgta acgtagtgta gtggttaaga
     6001 gcttggattt tgcagtaact tggattctaa tcctttaaaa tggggctgat atacctgcct
     6061 catattcaat gaaatattgt gtgtacagca cttagtaatt aatgaaactg gtaattatta
     6121 gcttagttac tgtaatgata atttgggacc ataaacacat atcaccctac attttctttg
     6181 caactacaat ttgtgaagga aaatctccca ctaactattg gttatgttaa tattcttcat
     6241 agagttgggt tcaagaagag aaatgtttta taatgaagca tataactatt ttataaacaa
     6301 cattgtagtt ataaagaagc atgcctatag tgttattaaa aattttttgt acgactttct
     6361 ggagctacaa gtaatgaact tgctttgaga gggtccagac atactcatcc tgaaccacat
     6421 tcctacaaca tatgcccaac aacctgcacc agagtactta tatcttaacc aaaaagtttc
     6481 acttcaatgg tggtgcttca caaaatcttc aaagtgcttt catgtgtatt gcctcatttg
     6541 agcttcaaat aaccttataa atcgggcagg tagatgttag tttacagagg aggagacagc
     6601 tttggaatag tggtgacgta tctgaagaat agaaaaccgg aatagtgctc gatgcctgaa
     6661 gtggtgcctg gcgccagcag atgttcactt ctgtcaaaca aggggaagta gtggtggagc
     6721 ctttattcag gcacgggtct cctcattcct aactccaaga tgcagaggga aaacgaactg
     6781 ctcaattaag agttcttcca tggacacttt cttaagcctt cacataaaat aacaagaata
     6841 atagatattt attgagtaga tatttattga gttctttcta ttttccagca ctcatctatt
     6901 aagtgctttt atgtattaac tcatttaatc cacacaacaa ccttatgtga gaaataccat
     6961 ttttccttat ttgattggtg aagaagcaca cagagagggt aagcataatc aaatacacca
     7021 gcataaccaa aatcacacat ttagcgagtg gcagggcctg gatgggagtc tgttcaactc
     7081 tctgctgtat tgcctcttaa agtaggtttt cttaaaacat aaatctttca taactttata
     7141 tatttatagt aacttaatcc aaagaacccc aaaagtacta gcaatgattg tttctgcaat
     7201 ttaaataaat ccatttaatt atacaacttt gcattatata ctgttattca gaccttggaa
     7261 cagtctataa ttgtttgtca aaagatttta tgtactactt tttcttaatt tcagatatta
     7321 tccagagttt ttcttggcag atggatgtat taaaaaaata gctttccagt gttagttctc
     7381 aacagtgctg tttcaaaagt tgttttaaaa gttctaacgg ttggtcttca aaacgtaatt
     7441 aagaccttat cataaagagc cttttgcaca ggactatctc aaaccctaag aagctattca
     7501 tatttaagaa aattttgttg tattaaatat tagtccaagt gagctctgga aatacagtct
     7561 tctgctttct tgtttcataa ataacactgg tatgcagacc cctccccatg actcaaatat
     7621 taatatatgc aattctgtta cagtaaagtt ttcaccatgg tcaacctatt ccagcattag
     7681 gtactttttg ttgttgttgt tttgtttttt tttttttttt ttgagatgga gtctcactct
     7741 gtcacccagg ctggagtgca agggtacaat ctcagctcac tgcaggctcc acctaccggg
     7801 ttcaagtgat cctcctgcct cagcctccca agtagctggc gctcaccact actggtgccc
     7861 accacaatgc ccggctaatt tttgtacttt tagtagaaac agggtttctg ttggccaggt
     7921 tggtctcgaa ctcctgacct catgtgatgc gcctgccttg gcctcccaaa gtgctgggat
     7981 tacaggcgtg agccaccgta cccagcctag cattaggtac tttgttttgt tttgttttgt
     8041 ttttgttttt tttgagatgg agttttgctc ttgttgccca ggctggagtg ccatggcaac
     8101 acagcctcca cttcctgtgt tcaagcaatt ctcctgcctc agcctcctga gtagctggga
     8161 ttacaggcgt ctgccaccac gcttagctga ttttttgtat ttttagtgga gatggggttt
     8221 cgccatgctt gccaggctgg tctcgaactc ctgacctcag gtgatctgcc tgccttggcc
     8281 ttccaaagtg ctgggattac aggcgtgagc cgctgcgccc agcgcactaa atactattaa
     8341 aaggaattaa gcaaaatgct acgtaccaat gattatgatt catgatcacc taaacacaga
     8401 atgcagactt aacttttcat tcttagtgtg ctaattaagt agtagtatgg tgcagtaagg
     8461 aattttgtta attttgttat aaactccctc tcctgaaatc cgtaaggaaa tgtgataata
     8521 agaaagtgat aatagtgtca tattttaaaa gtagaatcca ctgttaagca ccaagattac
     8581 cttttcttcc ccctgtggca cagtttattt aaatgaagta ttagcaataa tcatgtcact
     8641 attttgtcct gaataattaa gagtttgctt ttttcccatg tctttgcaat aggataatat
     8701 aaagaatagt attaaaagtc agaggcttta ctaatctacc tatatgtatt ccatggctaa
     8761 caaaccctgg cccctttaca tatgagctct ggaggttcgc ctggctgcct caggcttgca
     8821 gaaggctgcc ccaatcacag agcctgggta aggtggaaca ggaggcagcc ccactcggct
     8881 tttctgattg catcccacct gtttctgagt gtgttggttt ggtttaattc ttttcaaggg
     8941 ttggagttgg aaagtgaaaa ccctagacac ttgctgtgga atgtttgcct ggttgtattg
     9001 gtgtgtccct cttcttcact ggcatgtcgc tttcaagtgt accaaaggac attttgttct
     9061 gttgaaagcc acaggaccaa aaggaaaata ttgcaactat ttgcaaacat acttccctac
     9121 ctgtacaagc agccatatac taaaaagcac taaacaagca caaatgaaca ctaaatagcc
     9181 ttataccaaa aagcattctt gtaactgtca gggcatggta tgaattcctt cctctttaag
     9241 cagcaactta ccacaggctt ggtggcttta agtaatatag cattaagcaa atggtcagtt
     9301 attttttaat gttgaaaact tccaagtgtg aataatacgg acatagttta ctaccttttg
     9361 cttttaatat acctggttat ctatttccat ttgaaataaa atgaaaggag acctcaaact
     9421 gatgctgaga agtagacaaa atcagctctc agacttaact ctccccaatt aaaatagttt
     9481 ttttttccct tcccattttt ttgtttttaa gagatagggt cttgctgtgt tgcccaggct
     9541 agagtgcagc agtgacatga tcatagctca ctgcagcctg gaactcctgg gctcaggatc
     9601 cttctgcttc ggcctcctga gtagctaaga ccacaggtgt gtgccaccac acctggctaa
     9661 tttttttaaa attttttgta gagatgggga cttgctatgt tgcccagact ggtctcgaac
     9721 tcctggcctc aagccaccaa cctctcaaat tgctagaatt acaagcatga gccaccacac
     9781 ctggcctgtt attccttctt tatctaatgt gtgctaagct tgtgaaaaat atatgttgag
     9841 gtaaataggg caaaacatta gttgataaat tatgctaatt aatgggaaaa atagacatgt
     9901 tcctctctga acatttagaa ggactctgcc ctacaactat cttctgtttt tagaatttgt
     9961 agtcactgtt cttagtgcca ctggaaatat attcattctt tgagcatgta cagggtgggc
    10021 tccctgttgt atttattaca cttttcaaaa tgccagcaag tttttgtttg tatagagttg
    10081 gaatgtattg ttcgtgcatg cctgtgatat tcatcatcaa aatatacctg taaaaaataa
    10141 actactgctt cctctccaca gcttaggcct ccctcttact aaaaacaata gtagtttctg
    10201 tagaagtttc agtgagaaat tatggttata taaataacag atatggcaga acaattttgt
    10261 tgtagtattt ttttccgtag catttcttaa taatagctca gtttttaaag gaggggaaca
    10321 ataccccatg agttcaaatt aattttctct actttgaggt ataccttcct aattatattt
    10381 tacataggct gtttttttta agtttaaatt ctcactgtta agttgcattg agagacaatt
    10441 agaaatgttg taattgtcat atctttacat gtggattatg aacaaatgaa agtttgctgt
    10501 gtgattgcag ttttaaatta taacatttca taaatatgtc aattttagaa actcaaactc
    10561 tttcccatct tttgtatgga taaagtttat ggtttcattt ctgagaatag agttggtctg
    10621 ctgtgctaac ttcatgtttc ttattccaaa ggcttgatta tatttttttc tccagtgatt
    10681 aaaaatgcag cgaaaatcca atctacaagt tcatatattg gtatttctag acatagtcta
    10741 gttctaaaag aatgtacttg gtgtgcattt ttaagtgttt catgtagaca gattaatata
    10801 tttttgtaca acattgtatt tctacattta tttcaagact gtacttttca gtgacttttt
    10861 caagtgcatg tgttaacaga agattgtttg gaacgagagt gcagtggctt ctttactagc
    10921 aaagagaagt gtaatacaag tgatcataga aggtgagaat gtgtttatac tgtatatgga
    10981 aacctaatgc ctcttttcta aagctttgta catttttttc gtgaaataga ttaaatattt
    11041 tctctctaaa a
//
