LOCUS       NM_024782               8020 bp    mRNA    linear   PRI 08-FEB-2025
DEFINITION  Homo sapiens non-homologous end joining factor 1 (NHEJ1),
            transcript variant 1, mRNA.
ACCESSION   NM_024782
VERSION     NM_024782.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8020)
  AUTHORS   Seif-El-Dahan,M., Kefala-Stavridi,A., Frit,P., Hardwick,S.W.,
            Chirgadze,D.Y., Maia De Oliviera,T., Andreani,J., Britton,S.,
            Barboule,N., Bossaert,M., Pandurangan,A.P., Meek,K., Blundell,T.L.,
            Ropars,V., Calsou,P., Charbonnier,J.B. and Chaplin,A.K.
  TITLE     PAXX binding to the NHEJ machinery explains functional redundancy
            with XLF
  JOURNAL   Sci Adv 9 (22), eadg2834 (2023)
   PUBMED   37256950
  REMARK    GeneRIF: PAXX binding to the NHEJ machinery explains functional
            redundancy with XLF.
            Erratum:[Sci Adv. 2023 Sep 29;9(39):eadj8489. doi:
            10.1126/sciadv.adj8489.. PMID: 37774037]
REFERENCE   2  (bases 1 to 8020)
  AUTHORS   Jessulat,M., Amin,S., Hooshyar,M., Malty,R., Moutaoufik,M.T.,
            Zilocchi,M., Istace,Z., Phanse,S., Aoki,H., Omidi,K., Burnside,D.,
            Samanfar,B., Aly,K.A., Golshani,A. and Babu,M.
  TITLE     The conserved Tpk1 regulates non-homologous end joining
            double-strand break repair by phosphorylation of Nej1, a homolog of
            the human XLF
  JOURNAL   Nucleic Acids Res 49 (14), 8145-8160 (2021)
   PUBMED   34244791
  REMARK    GeneRIF: The conserved Tpk1 regulates non-homologous end joining
            double-strand break repair by phosphorylation of Nej1, a homolog of
            the human XLF.
REFERENCE   3  (bases 1 to 8020)
  AUTHORS   Chaplin,A.K., Hardwick,S.W., Stavridi,A.K., Buehl,C.J., Goff,N.J.,
            Ropars,V., Liang,S., De Oliveira,T.M., Chirgadze,D.Y., Meek,K.,
            Charbonnier,J.B. and Blundell,T.L.
  TITLE     Cryo-EM of NHEJ supercomplexes provides insights into DNA repair
  JOURNAL   Mol Cell 81 (16), 3400-3409 (2021)
   PUBMED   34352203
  REMARK    GeneRIF: Cryo-EM of NHEJ supercomplexes provides insights into DNA
            repair.
REFERENCE   4  (bases 1 to 8020)
  AUTHORS   Andres,S.N., Modesti,M., Tsai,C.J., Chu,G. and Junop,M.S.
  TITLE     Crystal structure of human XLF: a twist in nonhomologous DNA
            end-joining
  JOURNAL   Mol Cell 28 (6), 1093-1101 (2007)
   PUBMED   18158905
  REMARK    GeneRIF: Mutational analysis of XLF and XRCC4 reveals a potential
            interaction interface, suggesting a mechanism for how XLF
            stimulates the ligation of mismatched ends.
REFERENCE   5  (bases 1 to 8020)
  AUTHORS   Tsai,C.J., Kim,S.A. and Chu,G.
  TITLE     Cernunnos/XLF promotes the ligation of mismatched and noncohesive
            DNA ends
  JOURNAL   Proc Natl Acad Sci U S A 104 (19), 7851-7856 (2007)
   PUBMED   17470781
  REMARK    GeneRIF: Cernunnos/XRCC4-like factor promotes a mismatched end
            (MEnd) DNA ligase activity to facilitate joining and to preserve
            DNA sequence.
REFERENCE   6  (bases 1 to 8020)
  AUTHORS   Revy,P. and de Villartay,J.P.
  TITLE     [Cernunnos, a novel DNA repair factor essential for the immune
            system]
  JOURNAL   Med Sci (Paris) 22 (6-7), 569-570 (2006)
   PUBMED   16828027
  REMARK    GeneRIF: DNA repair protein involved in lymphocyte activation and
            cell division.
REFERENCE   7  (bases 1 to 8020)
  AUTHORS   Callebaut,I., Malivert,L., Fischer,A., Mornon,J.P., Revy,P. and de
            Villartay,J.P.
  TITLE     Cernunnos interacts with the XRCC4 x DNA-ligase IV complex and is
            homologous to the yeast nonhomologous end-joining factor Nej1
  JOURNAL   J Biol Chem 281 (20), 13857-13860 (2006)
   PUBMED   16571728
  REMARK    GeneRIF: Cernunnos physically interacts with the XRCC4 x DNA-ligase
            IV complex
REFERENCE   8  (bases 1 to 8020)
  AUTHORS   Ahnesorg,P., Smith,P. and Jackson,S.P.
  TITLE     XLF interacts with the XRCC4-DNA ligase IV complex to promote DNA
            nonhomologous end-joining
  JOURNAL   Cell 124 (2), 301-313 (2006)
   PUBMED   16439205
  REMARK    GeneRIF: Studies on the binding of XLF and its role in DNA repair.
            GeneRIF: The XLF-encoded protein (XRRC4 like factor, FLJ12610) is
            involved in DNA double-strand break repair via nonhomologous
            end-joining and associates with the Ligase IV-XRCC4 complex. XLF is
            mutated in a number of radiosensitive and immuno-deficient
            patients.
REFERENCE   9  (bases 1 to 8020)
  AUTHORS   Buck,D., Malivert,L., de Chasseval,R., Barraud,A., Fondaneche,M.C.,
            Sanal,O., Plebani,A., Stephan,J.L., Hufnagel,M., le Deist,F.,
            Fischer,A., Durandy,A., de Villartay,J.P. and Revy,P.
  TITLE     Cernunnos, a novel nonhomologous end-joining factor, is mutated in
            human immunodeficiency with microcephaly
  JOURNAL   Cell 124 (2), 287-299 (2006)
   PUBMED   16439204
  REMARK    GeneRIF: Cernunnos-XLF is a new Non-homologous End Joining pathway
            protein, which if mutated results in several conditions
            -immunodeficiency and developmental anomalies and other conditions,
            which likely result from inability to repair spontaneous DNA
            damage.
REFERENCE   10 (bases 1 to 8020)
  AUTHORS   Dai,Y., Kysela,B., Hanakahi,L.A., Manolis,K., Riballo,E., Stumm,M.,
            Harville,T.O., West,S.C., Oettinger,M.A. and Jeggo,P.A.
  TITLE     Nonhomologous end joining and V(D)J recombination require an
            additional factor
  JOURNAL   Proc Natl Acad Sci U S A 100 (5), 2462-2467 (2003)
   PUBMED   12604777
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DB124934.1, AJ972687.1 and
            AC097468.3.
            
            On Jan 16, 2020 this sequence version replaced NM_024782.2.
            
            Summary: Double-strand breaks in DNA result from genotoxic stresses
            and are among the most damaging of DNA lesions. This gene encodes a
            DNA repair factor essential for the nonhomologous end-joining
            pathway, which preferentially mediates repair of double-stranded
            breaks. Mutations in this gene cause different kinds of severe
            combined immunodeficiency disorders. [provided by RefSeq, Jul
            2008].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR14038197.525462.1,
                                           SRR14038191.2629853.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000356853.10/ ENSP00000349313.5
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      expression
            ##RefSeq-Attributes-END##
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-6                 DB124934.1         51-56
            7-2053              AJ972687.1         1-2047
            2054-8020           AC097468.3         148995-154961       c
FEATURES             Location/Qualifiers
     source          1..8020
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="2"
                     /map="2q35"
     gene            1..8020
                     /gene="NHEJ1"
                     /gene_synonym="IMD124; MCOPCB13; XLF"
                     /note="non-homologous end joining factor 1"
                     /db_xref="GeneID:79840"
                     /db_xref="HGNC:HGNC:25737"
                     /db_xref="MIM:611290"
     exon            1..96
                     /gene="NHEJ1"
                     /gene_synonym="IMD124; MCOPCB13; XLF"
                     /inference="alignment:Splign:2.1.0"
     CDS             97..996
                     /gene="NHEJ1"
                     /gene_synonym="IMD124; MCOPCB13; XLF"
                     /note="isoform 1 is encoded by transcript variant 1;
                     XRCC4-like factor; protein cernunnos; nonhomologous
                     end-joining factor 1"
                     /codon_start=1
                     /product="non-homologous end-joining factor 1 isoform 1"
                     /protein_id="NP_079058.1"
                     /db_xref="CCDS:CCDS2432.1"
                     /db_xref="GeneID:79840"
                     /db_xref="HGNC:HGNC:25737"
                     /db_xref="MIM:611290"
                     /translation="MEELEQGLLMQPWAWLQLAENSLLAKVFITKQGYALLVSDLQQV
                     WHEQVDTSVVSQRAKELNKRLTAPPAAFLCHLDNLLRPLLKDAAHPSEATFSCDCVAD
                     ALILRVRSELSGLPFYWNFHCMLASPSLVSQHLIRPLMGMSLALQCQVRELATLLHMK
                     DLEIQDYQESGATLIRDRLKTEPFEENSFLEQFMIEKLPEACSIGDGKPFVMNLQDLY
                     MAVTTQEVQVGQKHQGAGDPHTSNSASLQGIDSQCVNQPEQLVSSAPTLSAPEKESTG
                     TSGPLQRPQLSKVKRKKPRGLFS"
     exon            97..273
                     /gene="NHEJ1"
                     /gene_synonym="IMD124; MCOPCB13; XLF"
                     /inference="alignment:Splign:2.1.0"
     exon            274..486
                     /gene="NHEJ1"
                     /gene_synonym="IMD124; MCOPCB13; XLF"
                     /inference="alignment:Splign:2.1.0"
     exon            487..625
                     /gene="NHEJ1"
                     /gene_synonym="IMD124; MCOPCB13; XLF"
                     /inference="alignment:Splign:2.1.0"
     exon            626..684
                     /gene="NHEJ1"
                     /gene_synonym="IMD124; MCOPCB13; XLF"
                     /inference="alignment:Splign:2.1.0"
     exon            685..802
                     /gene="NHEJ1"
                     /gene_synonym="IMD124; MCOPCB13; XLF"
                     /inference="alignment:Splign:2.1.0"
     exon            803..921
                     /gene="NHEJ1"
                     /gene_synonym="IMD124; MCOPCB13; XLF"
                     /inference="alignment:Splign:2.1.0"
     exon            922..8020
                     /gene="NHEJ1"
                     /gene_synonym="IMD124; MCOPCB13; XLF"
                     /inference="alignment:Splign:2.1.0"
     regulatory      1385..1390
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NHEJ1"
                     /gene_synonym="IMD124; MCOPCB13; XLF"
                     /note="hexamer: AATAAA"
     polyA_site      1404
                     /gene="NHEJ1"
                     /gene_synonym="IMD124; MCOPCB13; XLF"
                     /note="major polyA site"
     regulatory      2035..2040
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NHEJ1"
                     /gene_synonym="IMD124; MCOPCB13; XLF"
                     /note="hexamer: ATTATA"
     regulatory      2040..2045
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NHEJ1"
                     /gene_synonym="IMD124; MCOPCB13; XLF"
                     /note="hexamer: AATAAG"
     polyA_site      2053
                     /gene="NHEJ1"
                     /gene_synonym="IMD124; MCOPCB13; XLF"
                     /note="major polyA site"
     polyA_site      2056
                     /gene="NHEJ1"
                     /gene_synonym="IMD124; MCOPCB13; XLF"
     regulatory      7998..8003
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NHEJ1"
                     /gene_synonym="IMD124; MCOPCB13; XLF"
                     /note="hexamer: ATTAAA"
     polyA_site      8020
                     /gene="NHEJ1"
                     /gene_synonym="IMD124; MCOPCB13; XLF"
ORIGIN      
        1 gaaagcggcc tcttactcta ggcctttcgg tttgcgcgag cgggcaggaa agcgtgcgtg
       61 cggctaagag agtgggcgct ctcgcggccg ctgacgatgg aagaactgga gcaaggcctg
      121 ttgatgcagc catgggcgtg gctacagctt gcagagaact ccctcttggc caaggttttt
      181 atcaccaagc agggctatgc cttgttggtt tcagatcttc aacaggtgtg gcatgaacag
      241 gtggacacta gtgtggtcag ccagcgagcc aaggagctga acaagcggct cactgctcct
      301 cctgcagctt tcctctgtca tttggataat ctccttcgcc cattgttgaa ggacgctgct
      361 caccctagcg aagctacctt ctcctgtgat tgtgtggcag atgcactgat tctacgggtg
      421 cgaagtgagc tctctggcct ccccttctat tggaatttcc actgcatgct agctagtcct
      481 tccctggtct cccaacattt gattcgtcct ctgatgggca tgagtctggc attacagtgc
      541 caagtgaggg agctagcaac gttacttcat atgaaagacc tagagatcca agactaccag
      601 gagagtgggg ctacgctgat tcgagatcga ttgaagacag aaccatttga agaaaattcc
      661 ttcttggaac aatttatgat agagaaactg ccagaggcat gcagcattgg tgatggaaag
      721 ccctttgtca tgaatctgca ggatctgtat atggcagtca ccacacaaga ggtccaagtg
      781 ggacagaagc atcaaggcgc tggagatcct catacctcaa acagtgcttc cctgcaagga
      841 atcgatagcc aatgtgtaaa ccagccagaa caactggtct cctcagcccc aaccctctca
      901 gcacctgaga aagagtccac gggtacttca ggccctctgc agagacctca gctgtcaaag
      961 gtcaagagga agaagccaag gggtctcttc agttaatctg ttgtggcctc agctgctgag
     1021 gatggacttg gagaacagct tccaagcttc accttgaaag aagcttacat ggcagcaata
     1081 tttctaaaat agtgatacag tcagaggcct cctgtaaggg cgagagaact gaagttgatg
     1141 ttgacaggcc cacagggaat tggccttccc tgttcaagtg gaagccagtc tctgagaatc
     1201 ccgtgctctc ctctcttttg gtggaggttc tgtaggttca ggtttctacc atggacttta
     1261 ggtatatagg gcaagtcagc aagaaagcac cacacactca ggaagccttg tctacctttc
     1321 cctagcgtct ctagccagcc agccccagat actcctcaga gacccacttc tctcttttgc
     1381 atggaataaa aagcactcac agtccctgct tttgggatta cttatgctgt ggaactcata
     1441 acccaattca cttccttcac tgggtcccac cccattgttg tctctggatg aagtctagcc
     1501 atcagtctgg tccttcagat tcttcgtaaa cttttctgca gttgccagag ctaagtctcc
     1561 atttggaaac tttgatctca agaactctct tgatggtggg cacaagacag ataggtgatt
     1621 gctgctttcc tttgccaaac ttggatgcat ttttctcttt gtttccaaag tataggcaac
     1681 aggttaaact accacgtggg gtagtttatc aatcctcccg cctcatcctt ctgcataact
     1741 gggactaaag gcacacacca ccacacctag ctaatttttg tatttttttg tgtgtggaga
     1801 caaggctttg ccatgttgcc caggctggtc ttgaactcct ggactctagt gatctgccca
     1861 cctcaacctc ccaaagtgct gggattacag gcatgagcta ccacgcctat ccaatttctg
     1921 gtttatttac tttcgatggt taaatgcctc cttattcaaa tatcctgtat atatactggc
     1981 gtttctgtcc tgatgtcaag tctcgagaga agatgatcaa acaataataa aataattata
     2041 ataagaacaa caataataaa tagctaacat ttattgagca gttaatgtat accagctact
     2101 ctaatgagca cttgacattg atcctcatag catcatatga ggaagatgct gttaacccat
     2161 taagaaattg gatcctgaag tgagtaagtg acttatcaaa ggtcatacag ctaataagtg
     2221 acagccagaa tttaagccca gacagctgag ttcttaacag cattattata tacactaatg
     2281 aaaacattta ttttccagaa aggacattag gttcttcttt ccagtctccc actgtaaacc
     2341 tccttatccc atgaccttgc ctcttttcaa taggatgtct gctccccagg ggctcttcaa
     2401 ggaaggagct cttgaggact ccaggactta ccctcatgct ttttcctgct agcagagtct
     2461 gagatctcca ccaaggctca ttcccagtca ctgctgataa cttttcttta cccccagacc
     2521 acaggaccct agagtgtgct gtgtggtagt gactgtcatc gaagtgaatt gggaactata
     2581 taaaaacagc ctatcagctg gcttactttg ctcttttgtt actttttttt ttttttaatg
     2641 gagtcttgct cttgttgcct aggctggagt acaatcgcac aattttggct cactgcaaac
     2701 tccacctcct gtgttcaagt gattctcctg cctcagcctc ccgagtagct gggattagat
     2761 gcctgccacc aagcccggct aatttttgta ttttttgtag agacagggtt tcaccatgtt
     2821 ggctaggctg gtctcgaact cctgacctca ggtgatctac ctgcctcggc ctcccaaagt
     2881 gctgggatta cacgtttgag ccactgtgcc tggccgatct ttcgtttctt ttcagcataa
     2941 tttagatagt cagatttcag tatctacaag gcacatggaa aatggaaagg cttcccctgc
     3001 aagcttggag aaaataccgg ttcttcaccc tgggcattcc ctgtactgta acagcacaga
     3061 ctcaagacat ttttcatcac agcggggcca gaggcctgtg aatccctcaa aattgtaggt
     3121 aaaatcttaa gagcaaaatt ttgttgatgt tctcaaaaag aatgtgtgac tttctaaaaa
     3181 ggttaagtaa gaatatagct agtaaccttc catccactgc ccccatcatc cccaaaggtt
     3241 gctgtctcca ttggggtatt gcttaaatac gttgttagag aaacattgct accagaggcc
     3301 ctgccacagg cattcttgcc ccgaagccct taccccgcaa agctaggggt agtggttgag
     3361 aatcattcat cctaaggcaa ctccagggaa atccctgctt tgacaaattg gcctagccag
     3421 gctgtattcc agccctgccc cctggcttgg gcccctccct gaggccccca gcctggccca
     3481 acccccactc tacatgcaca taaaagggtc gaattctcgt ggctgctcca taaattttat
     3541 tccgtaaata ttagttttcc ctgtgtttaa taaagcagtg gcccacctcc ccgcctaccc
     3601 gcccgctccc cggggctggg gctggggcca gggctggctg gtgggagaag ggactctttc
     3661 aattgctttg gagtattttt agaaaaaaaa ataatataag gtggtttaca cgagcaagcc
     3721 agcaaaccag gagcaggact tgggacctcg aggaagttcc tgcaggaact tgacctaaaa
     3781 agtagagaaa tagcgccacc tagattgcaa ggcctacttg gccttgccgg ctggtccccc
     3841 atagttagct aggacaggca tgctccctgc ttggagagaa agcctcatcc ctttccactc
     3901 tagctgggta gcacctcctt ttctcatgcc tcagtggggg cttccccagc aacccttaaa
     3961 agcagagctc aggctgggtc ctaaaagata tcactagggt cattctcttc tcttgtcacc
     4021 aggcagccag tctcccagat caaggaggat ttccaaggga tagtaaagtt attgagaagg
     4081 caggaagaaa gcttctagtc tccatctttc cctgattcct gagaagcgaa ttcctatggt
     4141 gtaaggtggc gaagagtgag atctgtgctt ctatgctctg aactggaaag agtctagcct
     4201 gttgagacca aaatccttcc acaggcctgg tgtagcgcac ccacagtgag cactggggga
     4261 aggagagatg ctcacatctc ttgatactca gccctactgc tgctcgtttt tatccagcgg
     4321 tacatttgga ctcccttttc tttccttcct tttcacttag acctcccatt ttcccaagcc
     4381 cttacctcct ccttagccca cgtttgttgg gagataagca ggggcccctt gtgtactctg
     4441 tacaaagctg gcgggaggag tggggaatgg aaaaggctgt gctgctcctc ctggtttcgt
     4501 tccttacatt gtcactagga gcattttcct ctctctgttt attctgtctc tcattttctc
     4561 ctacttacaa aactacacag attcgctcca gagagtatag agtaagccta aattctctta
     4621 atagcccttc ctcatgatat ggtaagctca ctttgtctgt tctgcttatg ctgttaatgt
     4681 agactgtgtt aatagacaca cttcctgtgc acttttctgt tttcatcctt tcattaggtt
     4741 atactctttg agaacagggt cagttttaac cctaactcag aaaatatcac ttcagggata
     4801 ttataccatt aacaaatata attttatgtc aactaggttt tgatacaatc aggatatgtt
     4861 tttctaagct ctactgtgaa gtagtttctg tcttctaatt tttcttgaag aaaaaactgt
     4921 tagaaaggaa taagtgacat actgcaagga agccaccaaa ctcagagctc aagaaccttg
     4981 gttcctcgtc tctgttacta atatccataa atgtatgggc tagcaaaatt gtaatggatt
     5041 acactaggcc cttagataca gaggggaatg caggaagaga aggaagccca tactttatag
     5101 gaggggggtt cccagctgca ttgatgccct ggaagctgct acagggtgct cttgtccagg
     5161 gcagctcatt gctctatgtt ttttccttgt gttcttggcc aaggcctcag aagatctcac
     5221 catgagcgga acatccagtg catggagggt atgaggtagc ttgtcttatg tgaggaagag
     5281 ggaccatagg gaatccttct ctatttgaac atctagaagt ccatctttct tgatctttca
     5341 tcccatcttc tgagactttt tttgtggtgg ccttttatgt atcctctcag ctctgggctc
     5401 tcagttctct atcctagcac ataggaagtc attaggtagc acccaagtat tctagcaaag
     5461 gcttcatcac tcttctgagc tattaagtcc caccccactc agtttctcta tttggagccc
     5521 aacagatttt gctatccttc cacccactca gcacccaact cttatgggtt ctcaagaatc
     5581 tgggccagcc taccagggga acccggaaga agcactggaa gagctccagg gtgtggggag
     5641 cacacagcca ttgactcacc tgggaagatt gtgctaggat tacttgtcct gctgggccaa
     5701 gggccagtag ttaaaggagc atgcagtgcc acactcttcc tctctgccct tactctgggg
     5761 cccaaaacag tagcagagtt agaattgggg agtagggttc aggggctgct gggagtccct
     5821 tctgaaatgt gttgtattta ctttttcata ttggcagctg gaaggggaag cccatcccag
     5881 ctcggcattg ttgtctaaca tggttaatca gatcctataa tcctgctgtg gaaatattgg
     5941 gcctcactca aaacaataga gaccgagaat attgtggtta aatttcaacc aacggcctcc
     6001 ctggcctgtt tgcctaaccc ctacccccct ctcccgggcc agagtcctcc tagctttatg
     6061 gacccctgat aggattgacg gccagtcaga aatgggtgac aggagagggg agaatggagt
     6121 aggggaaggg tgatcctttt gactggcact tcatcctggc ttttccctcc tacctcaggc
     6181 cttgaaggct gagcagggga tggtgaaagg ggttggagct ggtagggaag ccccacaggg
     6241 tgtgggtctc cactcaggga tcaagagcag tgctgttggg aatcatgcat ggtaaagaca
     6301 gcccttactc aagacagaaa aagcctgatg ccacatgaga agggaagact gccagtttca
     6361 tttagcattg ttgcctagcg gggatttcag ggcgtcctgt cacgtgagag gaatggggag
     6421 gcactagttg tggaaggcag agctggtaat aatttagatt ttgtttcata gataacagca
     6481 atactaataa cggtgtgatg acatttgaag gtgatactga tggccctact tgagtggttt
     6541 tctgatagtg gtaatgtctg ttgtgttgcc ctctttcata ggtgtgaatt aaatctggca
     6601 ggaaaattta catagttaga atagcaagac aaagggaata gatcatttca gaaggataag
     6661 ctgtacaatg gtattttgaa ttaaaaaaaa aaaagaaaga aaagattgag tgtctggatt
     6721 gtgctaaagg ggaaatttaa gactgggctc catggcttac gcctgtaatc ccagcacttt
     6781 gggaggctga ggcgggagga ttgcttgagg ctaggagttt gagaccagcc taggcaacat
     6841 ggcgaaaccc tgtctttgca aaacattttt aaaattagcg tggtgggcca ggcgcagtgg
     6901 ctcacgcctg taatcccagc actttgggag gctgaggcag gtggatcacc tgaggtcagg
     6961 aatttgagac cagcctggcc aacatggtga aaccccatct ctactaaaaa tacaaaaatt
     7021 agctggatgt ggtggcgcac acctgtaatc ccagctagtc tactcaggag gctgaggcag
     7081 gagaatcgct ggaacccggg aggcagaggc tgcagtgagc tgagatcgtg ccactgcact
     7141 ccaccctggg cgacagagca agactccatc tcaaaaataa aaataaaaaa aaaattagtg
     7201 tggtgatgtg cgcctgtagt cccaggtact ccggaggctg tggcagattg tgggcttatt
     7261 gaacacaggc agtttgtggc cattcagcaa ggagcataat gcccctgttg gtggtgatag
     7321 tgaataagca ctcagtgcag tcaataagta tataatttga gttaatgcat gtttaatgat
     7381 tcctgtttct tgttgaatgt ggatttctct atctcattcc caatacattt cctacaagcc
     7441 agtgccattc ccttgaattg ctttatagca atctggaatg ttttcccatt ggattttctc
     7501 acttgcagct caagtctgtg aacaccatga ggtttaatca acatgaggtt taagccaact
     7561 ttataggctt cctatatctc ccttcctggt cagcaatata gcccattctc tggagcttcc
     7621 cgtggggatt tttcttccca gtgaattctt cctattagtt atctaagtgg tttaactgtt
     7681 gaaatgtttt ccgtcatctt tagatccttt tgaaaccact ttctctacct gcttcagttc
     7741 catttgatga ccgccatcat ctttgagatt cccatccaga accatgcata cctcttaggt
     7801 tctcacagcc aggagctatt tcctaagttg tctttctggt ggaaagctca gctcctacta
     7861 agcagtcctt gcttcaaaat ggctttgatg aatgacccaa aggagccagg ccagtccctg
     7921 agtcatcaca gagcacccta ggtgagaaac cctgaagtgt tctctgctgt tgctccaccc
     7981 ctaccctata tgaacagatt aaatcgtttc gtttggcaca
//
