LOCUS       NM_002505               6209 bp    mRNA    linear   PRI 12-AUG-2024
DEFINITION  Homo sapiens nuclear transcription factor Y subunit alpha (NFYA),
            transcript variant 1, mRNA.
ACCESSION   NM_002505
VERSION     NM_002505.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 6209)
  AUTHORS   Sroka,M.W., Skopelitis,D., Vermunt,M.W., Preall,J.B., El
            Demerdash,O., de Almeida,L.M.N., Chang,K., Utama,R., Gryder,B.,
            Caligiuri,G., Ren,D., Nalbant,B., Milazzo,J.P., Tuveson,D.A.,
            Dobin,A., Hiebert,S.W., Stengel,K.R., Mantovani,R., Khan,J.,
            Kohli,R.M., Shi,J., Blobel,G.A. and Vakoc,C.R.
  TITLE     Myo-differentiation reporter screen reveals NF-Y as an activator of
            PAX3-FOXO1 in rhabdomyosarcoma
  JOURNAL   Proc Natl Acad Sci U S A 120 (36), e2303859120 (2023)
   PUBMED   37639593
  REMARK    GeneRIF: Myo-differentiation reporter screen reveals NF-Y as an
            activator of PAX3-FOXO1 in rhabdomyosarcoma.
REFERENCE   2  (bases 1 to 6209)
  AUTHORS   Okada,N., Ueki,C., Shimazaki,M., Tsujimoto,G., Kohno,S.,
            Muranaka,H., Yoshikawa,K. and Takahashi,C.
  TITLE     NFYA promotes malignant behavior of triple-negative breast cancer
            in mice through the regulation of lipid metabolism
  JOURNAL   Commun Biol 6 (1), 596 (2023)
   PUBMED   37268670
  REMARK    GeneRIF: NFYA promotes malignant behavior of triple-negative breast
            cancer in mice through the regulation of lipid metabolism.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 6209)
  AUTHORS   Li,Z., You,C., Huang,J. and Zhang,M.
  TITLE     NFYAv1 is a Tumor-Promoting Transcript Associated with Poor
            Prognosis of Hepatocellular Carcinoma
  JOURNAL   Med Sci Monit 29, e938410 (2023)
   PUBMED   36680333
  REMARK    GeneRIF: NFYAv1 is a Tumor-Promoting Transcript Associated with
            Poor Prognosis of Hepatocellular Carcinoma.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 6209)
  AUTHORS   Goos,H., Kinnunen,M., Salokas,K., Tan,Z., Liu,X., Yadav,L.,
            Zhang,Q., Wei,G.H. and Varjosalo,M.
  TITLE     Human transcription factor protein interaction networks
  JOURNAL   Nat Commun 13 (1), 766 (2022)
   PUBMED   35140242
  REMARK    Publication Status: Online-Only
REFERENCE   5  (bases 1 to 6209)
  AUTHORS   Han,F., Zhang,L., Liao,S., Zhang,Y., Qian,L., Hou,F., Gong,J.,
            Lai,M. and Zhang,H.
  TITLE     The interaction between S100A2 and KPNA2 mediates NFYA nuclear
            import and is a novel therapeutic target for colorectal cancer
            metastasis
  JOURNAL   Oncogene 41 (5), 657-670 (2022)
   PUBMED   34802034
  REMARK    GeneRIF: The interaction between S100A2 and KPNA2 mediates NFYA
            nuclear import and is a novel therapeutic target for colorectal
            cancer metastasis.
REFERENCE   6  (bases 1 to 6209)
  AUTHORS   Li,X.Y., Hooft van Huijsduijnen,R., Mantovani,R., Benoist,C. and
            Mathis,D.
  TITLE     Intron-exon organization of the NF-Y genes. Tissue-specific
            splicing modifies an activation domain
  JOURNAL   J Biol Chem 267 (13), 8984-8990 (1992)
   PUBMED   1577736
REFERENCE   7  (bases 1 to 6209)
  AUTHORS   Li,X.Y., Mantovani,R., Hooft van Huijsduijnen,R., Andre,I.,
            Benoist,C. and Mathis,D.
  TITLE     Evolutionary variation of the CCAAT-binding transcription factor
            NF-Y
  JOURNAL   Nucleic Acids Res 20 (5), 1087-1091 (1992)
   PUBMED   1549471
  REMARK    Erratum:[Nucleic Acids Res 1992 Apr 11;20(7):1841]
REFERENCE   8  (bases 1 to 6209)
  AUTHORS   Li,X.Y., Mattei,M.G., Zaleska-Rutczynska,Z., Hooft van
            Huijsduijnen,R., Figueroa,F., Nadeau,J., Benoist,C. and Mathis,D.
  TITLE     One subunit of the transcription factor NF-Y maps close to the
            major histocompatibility complex in murine and human chromosomes
  JOURNAL   Genomics 11 (3), 630-634 (1991)
   PUBMED   1774067
REFERENCE   9  (bases 1 to 6209)
  AUTHORS   Becker,D.M., Fikes,J.D. and Guarente,L.
  TITLE     A cDNA encoding a human CCAAT-binding protein cloned by functional
            complementation in yeast
  JOURNAL   Proc Natl Acad Sci U S A 88 (5), 1968-1972 (1991)
   PUBMED   2000400
REFERENCE   10 (bases 1 to 6209)
  AUTHORS   Vuorio,T., Maity,S.N. and de Crombrugghe,B.
  TITLE     Purification and molecular cloning of the 'A' chain of a rat
            heteromeric CCAAT-binding protein. Sequence identity with the yeast
            HAP3 transcription factor
  JOURNAL   J Biol Chem 265 (36), 22480-22486 (1990)
   PUBMED   2266139
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BM561405.1, X59711.1,
            AL031778.1 and BQ184804.1.
            
            On Jun 2, 2019 this sequence version replaced NM_002505.4.
            
            Summary: The protein encoded by this gene is one subunit of a
            trimeric complex, forming a highly conserved transcription factor
            that binds to CCAAT motifs in the promoter regions in a variety of
            genes. Subunit A associates with a tight dimer composed of the B
            and C subunits, resulting in a trimer that binds to DNA with high
            specificity and affinity. The sequence specific interactions of the
            complex are made by the A subunit, suggesting a role as the
            regulatory subunit. In addition, there is evidence of
            post-transcriptional regulation in this gene product, either by
            protein degradation or control of translation. Further regulation
            is represented by alternative splicing in the glutamine-rich
            activation domain, with clear tissue-specific preferences for the
            two isoforms. [provided by RefSeq, Jul 2008].
            
            Transcript Variant: This variant (1) includes alternate exon B,
            resulting in the long isoform, isoform 1.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803613.158135.1,
                                           SRR1803613.251019.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000341376.11/ ENSP00000345702.6
            RefSeq Select criteria :: based on manual assertion, conservation,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-43                BM561405.1         7-49
            44-1371             X59711.1           46-1373
            1372-5862           AL031778.1         143174-147664
            5863-6209           BQ184804.1         22-368              c
FEATURES             Location/Qualifiers
     source          1..6209
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="6"
                     /map="6p21.1"
     gene            1..6209
                     /gene="NFYA"
                     /gene_synonym="CBF-A; CBF-B; HAP2; NF-YA"
                     /note="nuclear transcription factor Y subunit alpha"
                     /db_xref="GeneID:4800"
                     /db_xref="HGNC:HGNC:7804"
                     /db_xref="MIM:189903"
     exon            1..111
                     /gene="NFYA"
                     /gene_synonym="CBF-A; CBF-B; HAP2; NF-YA"
                     /inference="alignment:Splign:2.1.0"
     exon            112..247
                     /gene="NFYA"
                     /gene_synonym="CBF-A; CBF-B; HAP2; NF-YA"
                     /inference="alignment:Splign:2.1.0"
     CDS             173..1216
                     /gene="NFYA"
                     /gene_synonym="CBF-A; CBF-B; HAP2; NF-YA"
                     /note="isoform 1 is encoded by transcript variant 1; HAP2
                     CCAAT-binding protein; Transcription factor NF-Y, A
                     subunit; CAAT-box DNA binding protein subunit A;
                     CCAAT-binding transcription factor subunit B; NFYA
                     transcript; nuclear transcription factor Y subunit A;
                     nuclear transcription factor Y, alpha"
                     /codon_start=1
                     /product="nuclear transcription factor Y subunit alpha
                     isoform 1"
                     /protein_id="NP_002496.1"
                     /db_xref="CCDS:CCDS4849.1"
                     /db_xref="GeneID:4800"
                     /db_xref="HGNC:HGNC:7804"
                     /db_xref="MIM:189903"
                     /translation="MEQYTANSNSSTEQIVVQAGQIQQQQQGGVTAVQLQTEAQVASA
                     SGQQVQTLQVVQGQPLMVQVSGGQLITSTGQPIMVQAVPGGQGQTIMQVPVSGTQGLQ
                     QIQLVPPGQIQIQGGQAVQVQGQQGQTQQIIIQQPQTAVTAGQTQTQQQIAVQGQQVA
                     QTAEGQTIVYQPVNADGTILQQVTVPVSGMITIPAASLAGAQIVQTGANTNTTSSGQG
                     TVTVTLPVAGNVVNSGGMVMMVPGAGSVPAIQRIPLPGAEMLEEEPLYVNAKQYHRIL
                     KRRQARAKLEAEGKIPKERRKYLHESRHRHAMARKRGEGGRFFSPKEKDSPHMQDPNQ
                     ADEEAMTQIIRVS"
     exon            248..334
                     /gene="NFYA"
                     /gene_synonym="CBF-A; CBF-B; HAP2; NF-YA"
                     /inference="alignment:Splign:2.1.0"
     exon            335..481
                     /gene="NFYA"
                     /gene_synonym="CBF-A; CBF-B; HAP2; NF-YA"
                     /inference="alignment:Splign:2.1.0"
     exon            482..613
                     /gene="NFYA"
                     /gene_synonym="CBF-A; CBF-B; HAP2; NF-YA"
                     /inference="alignment:Splign:2.1.0"
     exon            614..719
                     /gene="NFYA"
                     /gene_synonym="CBF-A; CBF-B; HAP2; NF-YA"
                     /inference="alignment:Splign:2.1.0"
     exon            720..886
                     /gene="NFYA"
                     /gene_synonym="CBF-A; CBF-B; HAP2; NF-YA"
                     /inference="alignment:Splign:2.1.0"
     exon            887..1060
                     /gene="NFYA"
                     /gene_synonym="CBF-A; CBF-B; HAP2; NF-YA"
                     /inference="alignment:Splign:2.1.0"
     exon            1061..1162
                     /gene="NFYA"
                     /gene_synonym="CBF-A; CBF-B; HAP2; NF-YA"
                     /inference="alignment:Splign:2.1.0"
     exon            1163..6209
                     /gene="NFYA"
                     /gene_synonym="CBF-A; CBF-B; HAP2; NF-YA"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3757..3762
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NFYA"
                     /gene_synonym="CBF-A; CBF-B; HAP2; NF-YA"
                     /note="hexamer: AATAAA"
     regulatory      3765..3770
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NFYA"
                     /gene_synonym="CBF-A; CBF-B; HAP2; NF-YA"
                     /note="hexamer: TATAAA"
     polyA_site      3775
                     /gene="NFYA"
                     /gene_synonym="CBF-A; CBF-B; HAP2; NF-YA"
                     /note="major polyA site"
     polyA_site      3782
                     /gene="NFYA"
                     /gene_synonym="CBF-A; CBF-B; HAP2; NF-YA"
     regulatory      6184..6189
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NFYA"
                     /gene_synonym="CBF-A; CBF-B; HAP2; NF-YA"
                     /note="hexamer: AATAAA"
     polyA_site      6209
                     /gene="NFYA"
                     /gene_synonym="CBF-A; CBF-B; HAP2; NF-YA"
ORIGIN      
        1 gctaggcagc ggcagtggcg gcggcagcgg cggctggagc ctctgattgg gtttcggagt
       61 ccggtactgg agccaatcag cgcgggcagc gaaccggggg agcgaggcac ggagtgtacc
      121 tcacagcctt ctaggatctc cagagtggac aggaatctca cttggaggga ccatggagca
      181 gtatacagca aacagcaata gttcgacaga gcagattgtt gtccaggcag gacagattca
      241 gcagcagcag cagggtggtg tcactgctgt gcagttgcag actgaggccc aggtggcatc
      301 cgcctcaggc cagcaagtcc agaccctcca ggtagtccaa gggcagccat taatggtgca
      361 ggtcagtgga ggccagctaa tcacatcaac tggccaaccc atcatggtcc aggctgtccc
      421 tggtggacaa ggtcaaacca tcatgcaagt acctgtttct ggaacacagg gtttgcagca
      481 aatacagttg gtcccacctg gacagatcca gatccagggt ggacaggctg tgcaggtgca
      541 gggccagcag ggccagaccc agcagatcat catccagcag ccccagacgg ctgtcactgc
      601 tggccagact cagacacagc agcagattgc tgtccaggga cagcaagtgg cacagactgc
      661 tgaagggcag accatcgtct atcaaccagt taatgcagat ggcaccattc tccagcaagt
      721 tacagtccct gtttcaggca tgatcactat cccagcagcc agtttggcag gagcacagat
      781 tgttcaaaca ggagccaata ccaacacaac cagcagtggg caagggactg tcactgtgac
      841 actaccagtg gcaggcaatg tggtcaattc aggagggatg gtcatgatgg ttcctggggc
      901 tggctctgtg cctgctatcc aaagaatccc tctacctgga gcagagatgc ttgaagaaga
      961 gcctctctac gtgaatgcca aacaatacca ccgtattctt aagaggaggc aagcccgagc
     1021 taaactagag gcagaaggga aaattccaaa ggagagaagg aaatacctgc atgagtctcg
     1081 gcaccgtcat gccatggcac ggaagcgtgg tgaaggtgga cgatttttct ctccaaagga
     1141 aaaggatagt ccccatatgc aggatccaaa ccaagccgat gaagaagcaa tgacacagat
     1201 catccgagtg tcctaacccc acgccatgtg atggagctga tcaaggtcat gtttctcact
     1261 gttccaggaa attgatcaac tcttccaatg ggacattgat gatcacattc tgccctttac
     1321 tacaggacag aaaccactta gtttttaata agtggctcag tattacaatt gcagcgatgc
     1381 ctggcaaatt gaagttgcaa gcctttgtct tactctttca gtcaggacct atttcagact
     1441 gttgatgaca ttgacatttc atggattcgg atgatgcaag gagacacatg ccaccccagc
     1501 agtacacaaa gcacttacct tgactggtga agtcagccag ccatctcagc agggaagaac
     1561 aaccttctca accaaaactg caatcaggaa agcagcagcc cactccttcc catggaatct
     1621 agacagcatt tatccctctg gtgggggaat ctcacactga ctaactgaat ggacgctttg
     1681 tattcagaga tggcttccag gcaaggaagt tctatcttgt gatacggaaa caataaattc
     1741 ctgagataca ttcttccaat ctgtggtaat aataagaatt cctctctgcc cagatggtag
     1801 gttgatattt ctggactcta gccaagaaaa agctaattct ggggatagaa tgataaaaga
     1861 ctcaagcact aaaaatatac attgccgaat ctctttttct agctttgaaa agttgtggcc
     1921 tgcttgtccc tctgttgact ggtcatctgg accatcgtac ttgctgtggc tacttctaag
     1981 acaatgtaga gggttattaa accttgaaac tgcctttcct aagtagagaa caagactatt
     2041 caacaacttc tttgctgaag cactgaggag atttgtaata ctcctaaagg aagggccaaa
     2101 ctagagattt tcaatcatag actttgtgac agcatttggg gaactaaaag attcatgtgt
     2161 ttcagcctag tgggagagag tgggggagag gaagagagag agagagcatg tatacccgta
     2221 tgttatcata gagcacgatt ctccagtgga tggatacctg gaatggatca ttaagatgaa
     2281 gagagtaatt cacatttact ctagaacctt taacaagcac tgaaaggaag aagcctgaga
     2341 tttgatcctt gacaatttct ggaaagcact ggtcagtcac actgtcctgg acagagtcca
     2401 agttacccac taaagcttga ggtggatgtt ggcttcactc ctgcagcaca atgctggccc
     2461 tagaaagcga gcattttcat tcttcccatg gcagctgatt ctttggtgcc agttttcatg
     2521 ttttctccat aggccttgtg cagaaaggtt ttctcattgc atccatactg tgagaataaa
     2581 ctgcagactg aaagaaacat gcctgcaggg tcaggaactg ggccactgtt cccagaacgg
     2641 gttgtgcctc agaaatctat ttctctttta gatgttgaac cctgagagaa ctaagatgat
     2701 atttcagaag agttggctgc cttctctgga aacaaggaaa acccaataat tccgaggaac
     2761 attgggggtt aaataccaag aataaaaatt ggttcttctt tgacttcagc ttaactgtat
     2821 taagacagaa attaagccct ctaaaaacac cacaaagaga gatgattgta atgatagaaa
     2881 tagaggtaca gagctatgca aaggaaacag gaagtgaaat attcctagtc ccagggatct
     2941 ggtaatcgct cctggcttcc agatgtcaaa ggactgatcc tttggtaagc tcactagaaa
     3001 cttctagctc tacaagagtt tgggatatta atgttattca gtaagtccca agtttttcag
     3061 agaatagatt actaacaatg aggaatttat attccttggt taatttagga gtctaagatg
     3121 cagagttcag aaagaaatta ctcagaccta actttgagtg cttaagctgc caacagttag
     3181 aagatgctat ttatttgtgt ctgtaagttt gtacatagta atgccagcct tgttgctgaa
     3241 ttttttattt ggggggagat gactttgaat tttgtttgaa atgcttcagt tgtcattttg
     3301 tcattgcatt tcaaatagct catgaccctt ttcttgtggt cactttggca caccatagta
     3361 gcccacaaag tgggggcagg gtattgacat ttggattttt tctttgatac tagttctttt
     3421 attcattttt atttgtgact gtagtgataa ctttacagat ggagaaagag tgaatggatg
     3481 aatgaataat tggtttaatg cctagttatg caacttgagt gctttttttt ttttttgcag
     3541 gataatttta agtgtatata ttttaactgc actggtacat tgaacatgtc agtgtcgatg
     3601 ccactggacc aacagagcat tttgtggcta tagggtgcct gataataatg tcttgatttt
     3661 tctttgggat tcaactatat cgaaggtttt tcccccagca gtatgggggc gtacaggtga
     3721 tcgatcatta atgtcattgc aattgttatt ttttaaaata aatttataaa aataaccgaa
     3781 aagtaatgtg acttttgagg aatctgcaac tcctgggttc tagtttgtgt ttccagtgtt
     3841 gaaattttga tctcccccga caagacttgt ctgcatggac ggtgtctctg taaacagtat
     3901 caaacttgta atgtctgagc ctcttagctt tatctcccct taatttcttc ccttttggcc
     3961 cttccaccct gttgaggaga ttttttctgt cacctgcagg ctgatcatac atttgatgct
     4021 actactgaaa gtgtttatca aaatgtgatg aagtaattcc atgaggaaaa ttagccgtta
     4081 tcacaaaaac agccttaagt attgtagaat ttcaggtagt gaagatgtac tatttacgtt
     4141 ttgttagatt ttgttttggc agaagaccaa aacaaaagta cttttctgat tgttaaaaat
     4201 ccacagcatg ctcaccttgt tactgtttta ccttacggtc gctgggaatc taagcaacaa
     4261 agactagaag cctccgatat gccattatca acacacaaga gacaaacagc ccgaggaagc
     4321 actcaatgat gagagcagta gacctgccct ggcagatgag agaggagaaa ctctccacaa
     4381 tgaaggaaaa gcactgacag tatttgatag ctttagtcaa actgtgcttg agtttggctt
     4441 agatttatgc cagtggaacc tgtccttacc aaattcagag atgacccaag attctactct
     4501 gtggtatgag ggaaagacct ctcgatattt atctcacacc aacggcttta tcgtaggagc
     4561 gtcttcgcct cccccgtttc cagtagaaaa gacagctttg ctcctttgaa agcgcagacc
     4621 gccgcacctc cagccccttc tccccgggga agtaggcccc gctaagaatg tgggaaggtg
     4681 gtggggcggc gactgaagtc gcttccgatt ggcgttgtcc caaggaagcc tgcgcggatt
     4741 gatcggcggc aggcctccaa tagagcctgc taggcggatt ggctgctacg cggctgggcc
     4801 ctgtttccgg tacctaggcg ggcagccatg gtgaccggcg agcggcatgc gacgccgcct
     4861 ctgtggcctg tggaggcccg cttggcggcg ctgcttcccg acctactggt ctttcggaag
     4921 cctcggggat gggaacccga gctcgccacg gcccagggcg tcctcctagg cgtccatgtg
     4981 acgggtgagg gcgacggccg gcacttgcac ttaagtctcc tggcctgcgg gagaggcggc
     5041 cgttgggtct aagcccctgg aggcctcgac ggttacaggc ctagcctccc gcgagctaag
     5101 gcacagttta acttcgtgct gcggcttcct taggaaactt tgagtatctt cgttttagcg
     5161 tgggaggaca tcttttggga tgaaccggct cagtccttga gctcacccca aagatgatga
     5221 aattgaaact cagaggaaag gatttttaca ggaccacgcc ccgggctttg aacccgtttt
     5281 tcccaacccc gagcattttc tattaagcgt tctgtttcaa ttattttatt cgttcagtta
     5341 attcgttctt ggcgtcataa gtatatgccc gccgttgttc tggccgtagc tatctagcag
     5401 ccactccgcg agtcgttcat ttaacaaata cttactctgt gtcagggata tgaagatggg
     5461 taataccagg tgcctgtcct cactgtgtga gtggttctct agccagacat gaggagggat
     5521 cctattgttt cccatagcta ggcagcctaa gagagtaggg gaaagagctg gctccagatg
     5581 aaaagggcac catctccagc tcttggaagc agagagggtg gtcctttgga ttcccagtgg
     5641 cagcagcaac caacctggcc cttagtagtt tctaagttac accatgttaa tggataaagg
     5701 aattactcag cttgaaggga tctgtttcct ttacactggc ctgatgtgtg gggagaaagg
     5761 ggccaaaaag tttaggattt ggagttaaca tcgtaatttc tgctttgttg gtcagggttt
     5821 acagcattaa tggacaggtt caccctgacc tacaagtcgg aacgtgacat ggacaaactg
     5881 gcccatatct tcagttatta cattagtgac atcgtggagc gtgagtgatc attgtagggt
     5941 aacaaggtgt ggtgctgggc tcctgggggt ttataaaaga agggggccag aagtacttta
     6001 aaacaggtac caaagggtca ttcctttagc atcattcatc tttggtttct tcaagttgcc
     6061 actgaaaagc tagacctttg cctattatct gctaatctac ccagtctcac attctattga
     6121 ctcattgttc aaaggggggg caggaggagc taatttcctt tgcacatttg tattttattt
     6181 caaaataaag tattctcttc taacaataa
//
