LOCUS       NM_021100               3008 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens NFS1 cysteine desulfurase (NFS1), transcript variant
            1, mRNA.
ACCESSION   NM_021100
VERSION     NM_021100.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3008)
  AUTHORS   Lin,J.F., Hu,P.S., Wang,Y.Y., Tan,Y.T., Yu,K., Liao,K., Wu,Q.N.,
            Li,T., Meng,Q., Lin,J.Z., Liu,Z.X., Pu,H.Y., Ju,H.Q., Xu,R.H. and
            Qiu,M.Z.
  TITLE     Phosphorylated NFS1 weakens oxaliplatin-based chemosensitivity of
            colorectal cancer by preventing PANoptosis
  JOURNAL   Signal Transduct Target Ther 7 (1), 54 (2022)
   PUBMED   35221331
  REMARK    GeneRIF: Phosphorylated NFS1 weakens oxaliplatin-based
            chemosensitivity of colorectal cancer by preventing PANoptosis.
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3008)
  AUTHORS   Morgenstern,M., Peikert,C.D., Lubbert,P., Suppanz,I., Klemm,C.,
            Alka,O., Steiert,C., Naumenko,N., Schendzielorz,A., Melchionda,L.,
            Muhlhauser,W.W.D., Knapp,B., Busch,J.D., Stiller,S.B.,
            Dannenmaier,S., Lindau,C., Licheva,M., Eickhorst,C., Galbusera,R.,
            Zerbes,R.M., Ryan,M.T., Kraft,C., Kozjak-Pavlovic,V., Drepper,F.,
            Dennerlein,S., Oeljeklaus,S., Pfanner,N., Wiedemann,N. and
            Warscheid,B.
  TITLE     Quantitative high-confidence human mitochondrial proteome and its
            dynamics in cellular context
  JOURNAL   Cell Metab 33 (12), 2464-2483 (2021)
   PUBMED   34800366
REFERENCE   3  (bases 1 to 3008)
  AUTHORS   Freibert,S.A., Boniecki,M.T., Stumpfig,C., Schulz,V., Krapoth,N.,
            Winge,D.R., Muhlenhoff,U., Stehling,O., Cygler,M. and Lill,R.
  TITLE     N-terminal tyrosine of ISCU2 triggers [2Fe-2S] cluster synthesis by
            ISCU2 dimerization
  JOURNAL   Nat Commun 12 (1), 6902 (2021)
   PUBMED   34824239
  REMARK    Publication Status: Online-Only
REFERENCE   4  (bases 1 to 3008)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   5  (bases 1 to 3008)
  AUTHORS   Herrera,M.G., Noguera,M.E., Sewell,K.E., Agudelo Suarez,W.A.,
            Capece,L., Klinke,S. and Santos,J.
  TITLE     Structure of the Human ACP-ISD11 Heterodimer
  JOURNAL   Biochemistry 58 (46), 4596-4609 (2019)
   PUBMED   31664822
REFERENCE   6  (bases 1 to 3008)
  AUTHORS   Deloukas,P., Matthews,L.H., Ashurst,J., Burton,J., Gilbert,J.G.,
            Jones,M., Stavrides,G., Almeida,J.P., Babbage,A.K., Bagguley,C.L.,
            Bailey,J., Barlow,K.F., Bates,K.N., Beard,L.M., Beare,D.M.,
            Beasley,O.P., Bird,C.P., Blakey,S.E., Bridgeman,A.M., Brown,A.J.,
            Buck,D., Burrill,W., Butler,A.P., Carder,C., Carter,N.P.,
            Chapman,J.C., Clamp,M., Clark,G., Clark,L.N., Clark,S.Y.,
            Clee,C.M., Clegg,S., Cobley,V.E., Collier,R.E., Connor,R.,
            Corby,N.R., Coulson,A., Coville,G.J., Deadman,R., Dhami,P.,
            Dunn,M., Ellington,A.G., Frankland,J.A., Fraser,A., French,L.,
            Garner,P., Grafham,D.V., Griffiths,C., Griffiths,M.N., Gwilliam,R.,
            Hall,R.E., Hammond,S., Harley,J.L., Heath,P.D., Ho,S., Holden,J.L.,
            Howden,P.J., Huckle,E., Hunt,A.R., Hunt,S.E., Jekosch,K.,
            Johnson,C.M., Johnson,D., Kay,M.P., Kimberley,A.M., King,A.,
            Knights,A., Laird,G.K., Lawlor,S., Lehvaslaiho,M.H., Leversha,M.,
            Lloyd,C., Lloyd,D.M., Lovell,J.D., Marsh,V.L., Martin,S.L.,
            McConnachie,L.J., McLay,K., McMurray,A.A., Milne,S., Mistry,D.,
            Moore,M.J., Mullikin,J.C., Nickerson,T., Oliver,K., Parker,A.,
            Patel,R., Pearce,T.A., Peck,A.I., Phillimore,B.J.,
            Prathalingam,S.R., Plumb,R.W., Ramsay,H., Rice,C.M., Ross,M.T.,
            Scott,C.E., Sehra,H.K., Shownkeen,R., Sims,S., Skuce,C.D.,
            Smith,M.L., Soderlund,C., Steward,C.A., Sulston,J.E., Swann,M.,
            Sycamore,N., Taylor,R., Tee,L., Thomas,D.W., Thorpe,A., Tracey,A.,
            Tromans,A.C., Vaudin,M., Wall,M., Wallis,J.M., Whitehead,S.L.,
            Whittaker,P., Willey,D.L., Williams,L., Williams,S.A., Wilming,L.,
            Wray,P.W., Hubbard,T., Durbin,R.M., Bentley,D.R., Beck,S. and
            Rogers,J.
  TITLE     The DNA sequence and comparative analysis of human chromosome 20
  JOURNAL   Nature 414 (6866), 865-871 (2001)
   PUBMED   11780052
REFERENCE   7  (bases 1 to 3008)
  AUTHORS   Stanchi,F., Bertocco,E., Toppo,S., Dioguardi,R., Simionati,B.,
            Cannata,N., Zimbello,R., Lanfranchi,G. and Valle,G.
  TITLE     Characterization of 16 novel human genes showing high similarity to
            yeast sequences
  JOURNAL   Yeast 18 (1), 69-80 (2001)
   PUBMED   11124703
REFERENCE   8  (bases 1 to 3008)
  AUTHORS   Tong,W.H. and Rouault,T.
  TITLE     Distinct iron-sulfur cluster assembly complexes exist in the
            cytosol and mitochondria of human cells
  JOURNAL   EMBO J 19 (21), 5692-5700 (2000)
   PUBMED   11060020
REFERENCE   9  (bases 1 to 3008)
  AUTHORS   Simpson,J.C., Wellenreuther,R., Poustka,A., Pepperkok,R. and
            Wiemann,S.
  TITLE     Systematic subcellular localization of novel proteins identified by
            large-scale cDNA sequencing
  JOURNAL   EMBO Rep 1 (3), 287-292 (2000)
   PUBMED   11256614
REFERENCE   10 (bases 1 to 3008)
  AUTHORS   Land,T. and Rouault,T.A.
  TITLE     Targeting of a human iron-sulfur cluster assembly enzyme, nifs, to
            different subcellular compartments is regulated through alternative
            AUG utilization
  JOURNAL   Mol Cell 2 (6), 807-815 (1998)
   PUBMED   9885568
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA082829.1, AK001265.1,
            AK223239.1, AK001470.1, R16676.1 and AL109827.8.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_021100.4.
            
            Summary: Iron-sulfur clusters are required for the function of many
            cellular enzymes. The proteins encoded by this gene supply
            inorganic sulfur to these clusters by removing the sulfur from
            cysteine, creating alanine in the process. This gene uses alternate
            in-frame translation initiation sites to generate mitochondrial
            forms and cytoplasmic/nuclear forms. Selection of the alternative
            initiation sites is determined by the cytosolic pH. The encoded
            proteins belong to the class-V family of pyridoxal
            phosphate-dependent aminotransferases. Alternatively spliced
            transcript variants have been described. [provided by RefSeq, Nov
            2010].
            
            Transcript Variant: This variant (1) encodes the longer isoform
            (a).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AF097025.2, AK312293.1 [ECO:0000332]
            RNAseq introns              :: mixed/partial sample support
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000350]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            gene product(s) localized to mito. :: reported by MitoCarta
            MANE Ensembl match                 :: ENST00000374092.9/
                                                  ENSP00000363205.3
            RefSeq Select criteria             :: based on conservation,
                                                  expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-1                 DA082829.1         14-14
            2-212               AK001265.1         1-211
            213-1939            AK223239.1         212-1938
            1940-2371           AK001470.1         1949-2380
            2372-2372           R16676.1           1-1                 c
            2373-3008           AL109827.8         73603-74238         c
FEATURES             Location/Qualifiers
     source          1..3008
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q11.22"
     gene            1..3008
                     /gene="NFS1"
                     /gene_synonym="COXPD52; HUSSY-08; IscS; NIFS"
                     /note="NFS1 cysteine desulfurase"
                     /db_xref="GeneID:9054"
                     /db_xref="HGNC:HGNC:15910"
                     /db_xref="MIM:603485"
     exon            1..161
                     /gene="NFS1"
                     /gene_synonym="COXPD52; HUSSY-08; IscS; NIFS"
                     /inference="alignment:Splign:2.1.0"
     CDS             65..1438
                     /gene="NFS1"
                     /gene_synonym="COXPD52; HUSSY-08; IscS; NIFS"
                     /EC_number="2.8.1.7"
                     /note="isoform a precursor is encoded by transcript
                     variant 1; nitrogen fixation 1 (S. cerevisiae, homolog);
                     nitrogen-fixing bacteria S-like protein; cysteine
                     desulfurase, mitochondrial; NFS1 nitrogen fixation 1
                     homolog"
                     /codon_start=1
                     /product="cysteine desulfurase isoform a precursor"
                     /protein_id="NP_066923.3"
                     /db_xref="CCDS:CCDS13262.1"
                     /db_xref="GeneID:9054"
                     /db_xref="HGNC:HGNC:15910"
                     /db_xref="MIM:603485"
                     /translation="MLLRAAWRRAAVAVTAAPGPKPAAPTRGLRLRVGDRAPQSAVPA
                     DTAAAPEVGPVLRPLYMDVQATTPLDPRVLDAMLPYLINYYGNPHSRTHAYGWESEAA
                     MERARQQVASLIGADPREIIFTSGATESNNIAIKGVARFYRSRKKHLITTQTEHKCVL
                     DSCRSLEAEGFQVTYLPVQKSGIIDLKELEAAIQPDTSLVSVMTVNNEIGVKQPIAEI
                     GRICSSRKVYFHTDAAQAVGKIPLDVNDMKIDLMSISGHKIYGPKGVGAIYIRRRPRV
                     RVEALQSGGGQERGMRSGTVPTPLVVGLGAACEVAQQEMEYDHKRISKLSERLIQNIM
                     KSLPDVVMNGDPKHHYPGCINLSFAYVEGESLLMALKDVALSSGSACTSASLEPSYVL
                     RAIGTDEDLAHSSIRFGIGRFTTEEEVDYTVEKCIQHVKRLREMSPLWEMVQDGIDLK
                     SIKWTQH"
     exon            162..271
                     /gene="NFS1"
                     /gene_synonym="COXPD52; HUSSY-08; IscS; NIFS"
                     /inference="alignment:Splign:2.1.0"
     exon            272..388
                     /gene="NFS1"
                     /gene_synonym="COXPD52; HUSSY-08; IscS; NIFS"
                     /inference="alignment:Splign:2.1.0"
     exon            389..472
                     /gene="NFS1"
                     /gene_synonym="COXPD52; HUSSY-08; IscS; NIFS"
                     /inference="alignment:Splign:2.1.0"
     exon            473..625
                     /gene="NFS1"
                     /gene_synonym="COXPD52; HUSSY-08; IscS; NIFS"
                     /inference="alignment:Splign:2.1.0"
     exon            626..719
                     /gene="NFS1"
                     /gene_synonym="COXPD52; HUSSY-08; IscS; NIFS"
                     /inference="alignment:Splign:2.1.0"
     exon            720..854
                     /gene="NFS1"
                     /gene_synonym="COXPD52; HUSSY-08; IscS; NIFS"
                     /inference="alignment:Splign:2.1.0"
     exon            855..1012
                     /gene="NFS1"
                     /gene_synonym="COXPD52; HUSSY-08; IscS; NIFS"
                     /inference="alignment:Splign:2.1.0"
     exon            1013..1118
                     /gene="NFS1"
                     /gene_synonym="COXPD52; HUSSY-08; IscS; NIFS"
                     /inference="alignment:Splign:2.1.0"
     exon            1119..1200
                     /gene="NFS1"
                     /gene_synonym="COXPD52; HUSSY-08; IscS; NIFS"
                     /inference="alignment:Splign:2.1.0"
     exon            1201..1284
                     /gene="NFS1"
                     /gene_synonym="COXPD52; HUSSY-08; IscS; NIFS"
                     /inference="alignment:Splign:2.1.0"
     exon            1285..1374
                     /gene="NFS1"
                     /gene_synonym="COXPD52; HUSSY-08; IscS; NIFS"
                     /inference="alignment:Splign:2.1.0"
     exon            1375..3008
                     /gene="NFS1"
                     /gene_synonym="COXPD52; HUSSY-08; IscS; NIFS"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2075..2080
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NFS1"
                     /gene_synonym="COXPD52; HUSSY-08; IscS; NIFS"
                     /note="hexamer: AATAAA"
     polyA_site      2095
                     /gene="NFS1"
                     /gene_synonym="COXPD52; HUSSY-08; IscS; NIFS"
                     /note="major polyA site"
     regulatory      2179..2184
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NFS1"
                     /gene_synonym="COXPD52; HUSSY-08; IscS; NIFS"
                     /note="hexamer: CATAAA"
     polyA_site      2197
                     /gene="NFS1"
                     /gene_synonym="COXPD52; HUSSY-08; IscS; NIFS"
     regulatory      2349..2354
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NFS1"
                     /gene_synonym="COXPD52; HUSSY-08; IscS; NIFS"
                     /note="hexamer: AATAAA"
     polyA_site      2372
                     /gene="NFS1"
                     /gene_synonym="COXPD52; HUSSY-08; IscS; NIFS"
     regulatory      2730..2735
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NFS1"
                     /gene_synonym="COXPD52; HUSSY-08; IscS; NIFS"
                     /note="hexamer: TATAAA"
     polyA_site      2767
                     /gene="NFS1"
                     /gene_synonym="COXPD52; HUSSY-08; IscS; NIFS"
     regulatory      2985..2990
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NFS1"
                     /gene_synonym="COXPD52; HUSSY-08; IscS; NIFS"
                     /note="hexamer: ATTAAA"
     polyA_site      3008
                     /gene="NFS1"
                     /gene_synonym="COXPD52; HUSSY-08; IscS; NIFS"
ORIGIN      
        1 acttccggga gcctggggcc caggactgca gcggcttcgg aaggtgggct ctgccagcgg
       61 gaccatgctg ctccgagccg cttggaggcg ggcggcagtg gcggtgacag cggctccagg
      121 gccgaagccc gcggcgccca ctcgggggct gcgcctgcgc gttggagacc gtgctcctca
      181 gtctgcggtt cccgcagata cagccgctgc cccggaggtg gggccagtgc tgcgacctct
      241 ctatatggat gtgcaagcta caactcctct ggacccccgg gtgcttgatg ccatgctccc
      301 ttacctaatc aactactatg ggaacccaca ctcccggaca catgcttatg gctgggagag
      361 tgaggcagcc atggaacgtg ctcgtcagca agtagcatct ctgattggag ctgatcctcg
      421 tgagatcatt tttactagtg gtgctactga atccaacaac atagcaatta agggggtggc
      481 ccgattctac aggtcacgga aaaagcactt gatcaccacc cagacagaac acaaatgtgt
      541 cttggactcc tgccgttcac tggaagctga gggctttcag gtcacctacc tcccagtgca
      601 gaagagtggg atcattgacc taaaggaact agaggctgct atccagccag atactagcct
      661 ggtgtcagtc atgactgtga acaatgagat tggagtgaag cagcctattg cagaaatagg
      721 gcggatttgc agttccagaa aggtatattt ccatactgat gcagcccagg ctgttggaaa
      781 aatcccactt gatgtcaatg acatgaaaat tgatctcatg agcattagtg gtcacaaaat
      841 ctacggtccc aaaggggttg gtgccatcta catccgtcgc cggccccgtg tgcgtgtgga
      901 ggccctgcag agtggagggg ggcaggagcg gggtatgcgg tctgggacag tgcccacacc
      961 cttagtggtg gggctggggg ctgcgtgtga ggtggcacag caagagatgg agtatgacca
     1021 caagcgaatc tcaaagttgt cagagcggct gatacagaat ataatgaaga gccttccaga
     1081 tgtggtgatg aatggggacc ctaagcacca ttatcccggc tgtatcaacc tctcctttgc
     1141 atatgtggaa ggggaaagtc tgctgatggc actgaaggac gttgccttat cctcagggag
     1201 tgcctgcacc tctgcatccc tggagccctc ttatgtgctt agagcaattg gcactgatga
     1261 ggatttagcg cactcttcta tcaggtttgg aattggccgc ttcactacag aggaggaagt
     1321 ggactacaca gtggagaaat gcattcagca tgtgaagcgt cttcgagaaa tgagccctct
     1381 ctgggagatg gttcaggatg gcattgacct caagagcatc aagtggaccc aacactagaa
     1441 gaatagggcc ctgactttgt gctggtctgg cccctcctgc ctcaccaacc cgtgcacaac
     1501 cagacacctt gttacaccta gtggatgctc tagattggta tagaccagtt gacttcagca
     1561 tcagtccacc tctatgacag aaacacaaga aaactgtctt tccctagctt cagttccttg
     1621 ggtgtggagc actccccatt tcttctcggg tcttaaagtg tgtggacatt ttcatcccga
     1681 agccatagag acatttgctg tcatattgct gctgggcaca tctgtgctct tggtgaggag
     1741 agcaagagga accagaagaa gtctctttgg tcagggacca tgatgctcta catggacatt
     1801 tgagtcttcg tcttctgctg ctgctcggct ggaccagctt ctttaacagc aagcataatc
     1861 cacttcaatg taatattttc tgtagctcca aaggctatct cttcatattg actgcagaca
     1921 gactgaatgg acagtttctt agagggcttg tctcctttct acccttgtcc tctttccttt
     1981 cctttgacct aatggagcta gaaatatgtc tgtgactcca ccagttattc taataatttg
     2041 ttttcttgaa aattgttaat ttcaagactg gagaaataaa ctcaccttct atttactctg
     2101 tgactttatg agaggttgag ggaaaagtgg aagctgtact tgggaattgt ggagaagacc
     2161 gccactccat ctgatttgca taaatgactc agtgggcttt tgtatctctt catttaagaa
     2221 ttatttgaat gcctgccagg agccagattt gttctgggta ttaggaatac actggttaac
     2281 aggaccaaaa aagaccctgc cctcactgag ttttaaattt tagtggagag atggaaaata
     2341 attaaacaaa taaacaatgt tatagattgt gataagtgct atgaaagaat taagtggtaa
     2401 gaggacatag cagggaaaac ctaccatgct gggtgtttga ggaaagtttt tctgaggagg
     2461 tagtgcttat aaatatgaag agctcgctaa tctagaaatg agaggtgaga aggaatggca
     2521 agagctaagg gcctgaagtg tgataaagca caaagtatta tactttagaa accaagagaa
     2581 ggccattgta actagagctt ttcatttgtg ggtctgaacc cataggtggg tagttaccag
     2641 tacttaaaaa aggagatagt atgttagagt acatttatgc atattttaag aatatatttt
     2701 gatgatatat ttgaatttgt ataccaggtt ataaatgtat ttcttattgt ggataatggt
     2761 caaaaaagtt taattgcttt tcatacttgc cagtagcagg gggacaggaa agtttgaaag
     2821 ccactgggct aaagtttggt aaatgtagat ggacagtgga aggaggagat gaaattatag
     2881 aatttttctt gttgaaaaat atctttttta cttagaataa ttttagattt actgaaaagt
     2941 tgcaaagata gtagagttgt agatctatta ccagttttcc tctaattaaa aagtcttaca
     3001 taaaacta
//
