LOCUS       NP_005375                462 aa            linear   PRI 27-APR-2025
DEFINITION  nuclear factor interleukin-3-regulated protein [Homo sapiens].
ACCESSION   NP_005375
VERSION     NP_005375.2
DBSOURCE    REFSEQ: accession NM_005384.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 462)
  AUTHORS   Qiang,F., Xuan,D., Liu,J. and Sheng,J.
  TITLE     Knockdown of NFIL3 modulates the AMPK pathway to suppress excessive
            cell proliferation, inflammation, and migration in rheumatoid
            arthritis
  JOURNAL   Int J Rheum Dis 27 (8), e15287 (2024)
   PUBMED   39175280
  REMARK    GeneRIF: Knockdown of NFIL3 modulates the AMPK pathway to suppress
            excessive cell proliferation, inflammation, and migration in
            rheumatoid arthritis.
REFERENCE   2  (residues 1 to 462)
  AUTHORS   Chen,S., Lei,M., Liu,K. and Min,J.
  TITLE     Structural basis for specific DNA sequence recognition by the
            transcription factor NFIL3
  JOURNAL   J Biol Chem 300 (3), 105776 (2024)
   PUBMED   38382670
  REMARK    GeneRIF: Structural basis for specific DNA sequence recognition by
            the transcription factor NFIL3.
REFERENCE   3  (residues 1 to 462)
  AUTHORS   Wang,L., Li,P., Zhang,X., Gu,Z., Pan,X., Wu,Y. and Li,H.
  TITLE     The role of basic leucine zipper transcription factor E4BP4 in
            cancer: a review and update
  JOURNAL   Mol Biol Rep 51 (1), 91 (2024)
   PUBMED   38193973
  REMARK    GeneRIF: The role of basic leucine zipper transcription factor
            E4BP4 in cancer: a review and update.
            Review article
            Publication Status: Online-Only
REFERENCE   4  (residues 1 to 462)
  AUTHORS   Zeng,G.G., Zhou,J., Jiang,W.L., Yu,J., Nie,G.Y., Li,J., Zhang,S.Q.
            and Tang,C.K.
  TITLE     A Potential Role of NFIL3 in Atherosclerosis
  JOURNAL   Curr Probl Cardiol 49 (1 Pt B), 102096 (2024)
   PUBMED   37741601
  REMARK    GeneRIF: A Potential Role of NFIL3 in Atherosclerosis.
            Review article
REFERENCE   5  (residues 1 to 462)
  AUTHORS   Chen,L., Shangguan,Z., Dong,Z., Deng,Q., Ding,Y. and Yang,S.
  TITLE     NFIL3 aggravates human coronary artery endothelial cell injury by
            promoting ITGAM transcription in Kawasaki disease
  JOURNAL   Hematology 28 (1), 2277502 (2023)
   PUBMED   37933872
  REMARK    GeneRIF: NFIL3 aggravates human coronary artery endothelial cell
            injury by promoting ITGAM transcription in Kawasaki disease.
REFERENCE   6  (residues 1 to 462)
  AUTHORS   Cowell,I.G. and Hurst,H.C.
  TITLE     Protein-protein interaction between the transcriptional repressor
            E4BP4 and the TBP-binding protein Dr1
  JOURNAL   Nucleic Acids Res 24 (18), 3607-3613 (1996)
   PUBMED   8836190
REFERENCE   7  (residues 1 to 462)
  AUTHORS   Zhang,W., Zhang,J., Kornuc,M., Kwan,K., Frank,R. and Nimer,S.D.
  TITLE     Molecular cloning and characterization of NF-IL3A, a
            transcriptional activator of the human interleukin-3 promoter
  JOURNAL   Mol Cell Biol 15 (11), 6055-6063 (1995)
   PUBMED   7565758
REFERENCE   8  (residues 1 to 462)
  AUTHORS   Matoba,R., Okubo,K., Hori,N., Fukushima,A. and Matsubara,K.
  TITLE     The addition of 5'-coding information to a 3'-directed cDNA library
            improves analysis of gene expression
  JOURNAL   Gene 146 (2), 199-207 (1994)
   PUBMED   8076819
REFERENCE   9  (residues 1 to 462)
  AUTHORS   Cowell,I.G., Skinner,A. and Hurst,H.C.
  TITLE     Transcriptional repression by a novel member of the bZIP family of
            transcription factors
  JOURNAL   Mol Cell Biol 12 (7), 3070-3077 (1992)
   PUBMED   1620116
REFERENCE   10 (residues 1 to 462)
  AUTHORS   Rehfuss,R.P., Walton,K.M., Loriaux,M.M. and Goodman,R.H.
  TITLE     The cAMP-regulated enhancer-binding protein ATF-1 activates
            transcription in response to cAMP-dependent protein kinase A
  JOURNAL   J Biol Chem 266 (28), 18431-18434 (1991)
   PUBMED   1655749
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from BQ937243.1, AW163045.1 and
            BC008197.1.
            
            On Sep 24, 2004 this sequence version replaced NP_005375.1.
            
            Summary: The protein encoded by this gene is a transcriptional
            regulator that binds as a homodimer to activating transcription
            factor (ATF) sites in many cellular and viral promoters. The
            encoded protein represses PER1 and PER2 expression and therefore
            plays a role in the regulation of circadian rhythm. Three
            transcript variants encoding the same protein have been found for
            this gene. [provided by RefSeq, Feb 2014].
            
            Transcript Variant: This variant (3) differs in the 5' UTR compared
            to variant 1. All three variants encode the same protein.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: ERR279872.3696.1, ERR279858.4433.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1966682, SAMEA2162895
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000297689.4/ ENSP00000297689.2
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..462
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q22.31"
     Protein         1..462
                     /product="nuclear factor interleukin-3-regulated protein"
                     /note="interleukin-3 promoter transcriptional activator;
                     E4 promoter-binding protein 4; interleukin-3-binding
                     protein 1; transcriptional activator NF-IL3A; adenovirus
                     E4 promoter region binding protein"
                     /calculated_mol_wt=51341
     Region          72..131
                     /region_name="bZIP_NFIL3"
                     /note="Basic leucine zipper (bZIP) domain of Nuclear
                     factor interleukin-3-regulated protein (NFIL3): a
                     DNA-binding and dimerization domain; cd14694"
                     /db_xref="CDD:269842"
     Region          73..131
                     /region_name="coiled coil"
                     /note="coiled coil [structural motif]"
                     /db_xref="CDD:269842"
     Region          79..95
                     /region_name="Basic motif.
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU00978"
                     /note="propagated from UniProtKB/Swiss-Prot (Q16649.2)"
     Site            order(79..80,82..84,86..91,93..95)
                     /site_type="DNA binding"
                     /note="DNA binding site [nucleotide binding]"
                     /db_xref="CDD:269842"
     Site            order(94,97..98,101..102,104..105,108..109,111..112,
                     115..116,118..119,122..123,125..126)
                     /site_type="other"
                     /note="dimer interface [polypeptide binding]"
                     /db_xref="CDD:269842"
     Region          99..106
                     /region_name="Leucine-zipper.
                     /evidence=ECO:0000255|PROSITE-ProRule:PRU00978"
                     /note="propagated from UniProtKB/Swiss-Prot (Q16649.2)"
     Region          130..461
                     /region_name="Vert_IL3-reg_TF"
                     /note="Vertebrate interleukin-3 regulated transcription
                     factor; pfam06529"
                     /db_xref="CDD:368956"
     Region          189..237
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q16649.2)"
     Region          258..302
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q16649.2)"
     Region          299..363
                     /region_name="Necessary for transcriptional repression and
                     sufficient for interaction with DR1.
                     /evidence=ECO:0000269|PubMed:8836190"
                     /note="propagated from UniProtKB/Swiss-Prot (Q16649.2)"
     Site            301
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:17081983,
                     ECO:0007744|PubMed:20068231, ECO:0007744|PubMed:23186163;
                     propagated from UniProtKB/Swiss-Prot (Q16649.2)"
     Site            353
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:20068231,
                     ECO:0007744|PubMed:23186163; propagated from
                     UniProtKB/Swiss-Prot (Q16649.2)"
     CDS             1..462
                     /gene="NFIL3"
                     /gene_synonym="E4BP4; IL3BP1; NF-IL3A; NFIL3A"
                     /coded_by="NM_005384.3:366..1754"
                     /db_xref="CCDS:CCDS6690.1"
                     /db_xref="GeneID:4783"
                     /db_xref="HGNC:HGNC:7787"
                     /db_xref="MIM:605327"
ORIGIN      
        1 mqlrkmqtvk keqasldass nvdkmmvlns altevsedst tgeelllseg svgknkssac
       61 rrkrefipde kkdamywekr rknneaakrs rekrrlndlv lenklialge enatlkaell
      121 slklkfglis stayaqeiqk lsnstavyfq dyqtsksnvs sfvdehepsm vssscisvik
      181 hspqsslsdv sevssvehtq essvqgscrs penkfqiikq epmelesytr eprddrgsyt
      241 asiyqnymgn sfsgyshspp llqvnrsssn sprtsetddg vvgkssdged eqqvpkgpih
      301 spvelkhvha tvvkvpevns salphklrik akamqikvea fdnefeatqk lsspidmtsk
      361 rhfelekhsa psmvhssltp fsvqvtniqd wslksehwhq kelsgktqns fktgvvemkd
      421 sgykvsdpen lylkqgianl saevvslkrl iatqpisasd sg
//
