LOCUS       NM_001005388           10336 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens neurofascin (NFASC), transcript variant 1, mRNA.
ACCESSION   NM_001005388 XM_046808
VERSION     NM_001005388.3
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 10336)
  AUTHORS   Thibord,F., Chan,M.V., Chen,M.H. and Johnson,A.D.
  TITLE     A year of COVID-19 GWAS results from the GRASP portal reveals
            potential genetic risk factors
  JOURNAL   HGG Adv 3 (2), 100095 (2022)
   PUBMED   35224516
REFERENCE   2  (bases 1 to 10336)
  AUTHORS   Shelton,J.F., Shastri,A.J., Ye,C., Weldon,C.H.,
            Filshtein-Sonmez,T., Coker,D., Symons,A., Esparza-Gordillo,J.,
            Aslibekyan,S. and Auton,A.
  CONSRTM   23andMe COVID-19 Team
  TITLE     Trans-ancestry analysis reveals genetic and nongenetic associations
            with COVID-19 susceptibility and severity
  JOURNAL   Nat Genet 53 (6), 801-808 (2021)
   PUBMED   33888907
REFERENCE   3  (bases 1 to 10336)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   4  (bases 1 to 10336)
  AUTHORS   Harper,J.L., Wilson,T.E. and Mitchell,R.M.
  TITLE     Case report of two children with auditory neuropathy spectrum
            disorder related to a neurofascin (NFASC) gene variant
  JOURNAL   Int J Pediatr Otorhinolaryngol 131, 109863 (2020)
   PUBMED   31945734
  REMARK    GeneRIF: Case report of two children with auditory neuropathy
            spectrum disorder related to a neurofascin (NFASC) gene variant.
REFERENCE   5  (bases 1 to 10336)
  AUTHORS   Tuvia,S., Garver,T.D. and Bennett,V.
  TITLE     The phosphorylation state of the FIGQY tyrosine of neurofascin
            determines ankyrin-binding activity and patterns of cell
            segregation
  JOURNAL   Proc Natl Acad Sci U S A 94 (24), 12957-12962 (1997)
   PUBMED   9371782
REFERENCE   6  (bases 1 to 10336)
  AUTHORS   Davis,J.Q., Lambert,S. and Bennett,V.
  TITLE     Molecular composition of the node of Ranvier: identification of
            ankyrin-binding cell adhesion molecules neurofascin (mucin+/third
            FNIII domain-) and NrCAM at nodal axon segments
  JOURNAL   J Cell Biol 135 (5), 1355-1367 (1996)
   PUBMED   8947556
REFERENCE   7  (bases 1 to 10336)
  AUTHORS   Volkmer,H., Leuschner,R., Zacharias,U. and Rathjen,F.G.
  TITLE     Neurofascin induces neurites by heterophilic interactions with
            axonal NrCAM while NrCAM requires F11 on the axonal surface to
            extend neurites
  JOURNAL   J Cell Biol 135 (4), 1059-1069 (1996)
   PUBMED   8922386
REFERENCE   8  (bases 1 to 10336)
  AUTHORS   Hortsch,M.
  TITLE     The L1 family of neural cell adhesion molecules: old proteins
            performing new tricks
  JOURNAL   Neuron 17 (4), 587-593 (1996)
   PUBMED   8893017
  REMARK    Review article
REFERENCE   9  (bases 1 to 10336)
  AUTHORS   Burmeister,M., Ren,Q., Makris,G.J., Samson,D. and Bennett,V.
  TITLE     Genes for the neuronal immunoglobulin domain cell adhesion
            molecules neurofascin and Nr-CAM map to mouse chromosomes 1 and 12
            and homologous human chromosomes
  JOURNAL   Mamm Genome 7 (7), 558-559 (1996)
   PUBMED   8672144
REFERENCE   10 (bases 1 to 10336)
  AUTHORS   Volkmer,H., Hassel,B., Wolff,J.M., Frank,R. and Rathjen,F.G.
  TITLE     Structure of the axonal surface recognition molecule neurofascin
            and its relationship to a neural subgroup of the immunoglobulin
            superfamily
  JOURNAL   J Cell Biol 118 (1), 149-161 (1992)
   PUBMED   1377696
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC096675.2 and AL391822.12.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Feb 7, 2020 this sequence version replaced NM_001005388.2.
            
            Summary: This gene encodes an L1 family immunoglobulin cell
            adhesion molecule with multiple IGcam and fibronectin domains. The
            protein functions in neurite outgrowth, neurite fasciculation, and
            organization of the axon initial segment (AIS) and nodes of Ranvier
            on axons during early development. Both the AIS and nodes of
            Ranvier contain high densities of voltage-gated Na+ (Nav) channels
            which are clustered by interactions with cytoskeletal and
            scaffolding proteins including this protein, gliomedin, ankyrin 3
            (ankyrin-G), and betaIV spectrin. This protein links the AIS
            extracellular matrix to the intracellular cytoskeleton. This gene
            undergoes extensive alternative splicing, and the full-length
            nature of some variants has not been determined.[provided by
            RefSeq, May 2009].
            
            Transcript Variant: This variant (1) encodes isoform 1. This
            isoform lacks the third fibronectin type 3 (FNIII) repeat and
            includes the mucin-like domain. Isoform 1 is also known as NF186,
            as described by Davis et al. (PMID: 8947556).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1803617.189883.1 [ECO:0000332]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: downstream AUG is associated
                                                with N-terminal localization
                                                signal
            coronavirus related              :: locus in the vicinity of
                                                disease-associated variant(s)
            MANE Ensembl match               :: ENST00000339876.11/
                                                ENSP00000344786.6
            RefSeq Select criteria           :: based on manual assertion,
                                                expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-131               AC096675.2         85919-86049
            132-240             AL391822.12        9432-9540
            241-421             AL391822.12        33026-33206
            422-439             AL391822.12        39357-39374
            440-545             AL391822.12        40811-40916
            546-742             AL391822.12        42988-43184
            743-865             AL391822.12        43629-43751
            866-1036            AL391822.12        46456-46626
            1037-1148           AL391822.12        57049-57160
            1149-1333           AL391822.12        57598-57782
            1334-1465           AL391822.12        59416-59547
            1466-1609           AL391822.12        62076-62219
            1610-1721           AL391822.12        62979-63090
            1722-1888           AL391822.12        63457-63623
            1889-2036           AL391822.12        64071-64218
            2037-2161           AL391822.12        65471-65595
            2162-2206           AL391822.12        66481-66525
            2207-2308           AL391822.12        67768-67869
            2309-2506           AL391822.12        68162-68359
            2507-2577           AL391822.12        69170-69240
            2578-2800           AL391822.12        70598-70820
            2801-2923           AL391822.12        76218-76340
            2924-3097           AL391822.12        77433-77606
            3098-3112           AL391822.12        80092-80106
            3113-3349           AL391822.12        85970-86206
            3350-3466           AL391822.12        89970-90086
            3467-3619           AL391822.12        91396-91548
            3620-3751           AL391822.12        98357-98488
            3752-3821           AL391822.12        101597-101666
            3822-10336          AL391822.12        105108-111622
FEATURES             Location/Qualifiers
     source          1..10336
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="1"
                     /map="1q32.1"
     gene            1..10336
                     /gene="NFASC"
                     /gene_synonym="NEDCPMD; NF; NRCAML"
                     /note="neurofascin"
                     /db_xref="GeneID:23114"
                     /db_xref="HGNC:HGNC:29866"
                     /db_xref="MIM:609145"
     exon            1..131
                     /gene="NFASC"
                     /gene_synonym="NEDCPMD; NF; NRCAML"
                     /inference="alignment:Splign:2.1.0"
     exon            132..240
                     /gene="NFASC"
                     /gene_synonym="NEDCPMD; NF; NRCAML"
                     /inference="alignment:Splign:2.1.0"
     exon            241..421
                     /gene="NFASC"
                     /gene_synonym="NEDCPMD; NF; NRCAML"
                     /inference="alignment:Splign:2.1.0"
     CDS             331..4053
                     /gene="NFASC"
                     /gene_synonym="NEDCPMD; NF; NRCAML"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1; neurofascin homolog"
                     /codon_start=1
                     /product="neurofascin isoform 1 precursor"
                     /protein_id="NP_001005388.2"
                     /db_xref="CCDS:CCDS53460.1"
                     /db_xref="GeneID:23114"
                     /db_xref="HGNC:HGNC:29866"
                     /db_xref="MIM:609145"
                     /translation="MARQPPPPWVHAAFLLCLLSLGGAIEIPMDPSIQNELTQPPTIT
                     KQSAKDHIVDPRDNILIECEAKGNPAPSFHWTRNSRFFNIAKDPRVSMRRRSGTLVID
                     FRSGGRPEEYEGEYQCFARNKFGTALSNRIRLQVSKSPLWPKENLDPVVVQEGAPLTL
                     QCNPPPGLPSPVIFWMSSSMEPITQDKRVSQGHNGDLYFSNVMLQDMQTDYSCNARFH
                     FTHTIQQKNPFTLKVLTTRGVAERTPSFMYPQGTASSQMVLRGMDLLLECIASGVPTP
                     DIAWYKKGGDLPSDKAKFENFNKALRITNVSEEDSGEYFCLASNKMGSIRHTISVRVK
                     AAPYWLDEPKNLILAPGEDGRLVCRANGNPKPTVQWMVNGEPLQSAPPNPNREVAGDT
                     IIFRDTQISSRAVYQCNTSNEHGYLLANAFVSVLDVPPRMLSPRNQLIRVILYNRTRL
                     DCPFFGSPIPTLRWFKNGQGSNLDGGNYHVYENGSLEIKMIRKEDQGIYTCVATNILG
                     KAENQVRLEVKDPTRIYRMPEDQVARRGTTVQLECRVKHDPSLKLTVSWLKDDEPLYI
                     GNRMKKEDDSLTIFGVAERDQGSYTCVASTELDQDLAKAYLTVLADQATPTNRLAALP
                     KGRPDRPRDLELTDLAERSVRLTWIPGDANNSPITDYVVQFEEDQFQPGVWHDHSKYP
                     GSVNSAVLRLSPYVNYQFRVIAINEVGSSHPSLPSERYRTSGAPPESNPGDVKGEGTR
                     KNNMEITWTPMNATSAFGPNLRYIVKWRRRETREAWNNVTVWGSRYVVGQTPVYVPYE
                     IRVQAENDFGKGPEPESVIGYSGEDLPSAPRRFRVRQPNLETINLEWDHPEHPNGIMI
                     GYTLKYVAFNGTKVGKQIVENFSPNQTKFTVQRTDPVSRYRFTLSARTQVGSGEAVTE
                     ESPAPPNEATPTAAPPTLPPTTVGATGAVSSTDATAIAATTEATTVPIIPTVAPTTIA
                     TTTTVATTTTTTAAATTTTESPPTTTSGTKIHESAPDEQSIWNVTVLPNSKWANITWK
                     HNFGPGTDFVVEYIDSNHTKKTVPVKAQAQPIQLTDLYPGMTYTLRVYSRDNEGISST
                     VITFMTSTAYTNNQADIATQGWFIGLMCAIALLVLILLIVCFIKRSRGGKYPVREKKD
                     VPLGPEDPKEEDGSFDYSDEDNKPLQGSQTSLDGTIKQQESDDSLVDYGEGGEGQFNE
                     DGSFIGQYTVKKDKEETEGNESSEATSPVNAIYSLA"
     exon            422..439
                     /gene="NFASC"
                     /gene_synonym="NEDCPMD; NF; NRCAML"
                     /inference="alignment:Splign:2.1.0"
     exon            440..545
                     /gene="NFASC"
                     /gene_synonym="NEDCPMD; NF; NRCAML"
                     /inference="alignment:Splign:2.1.0"
     exon            546..742
                     /gene="NFASC"
                     /gene_synonym="NEDCPMD; NF; NRCAML"
                     /inference="alignment:Splign:2.1.0"
     exon            743..865
                     /gene="NFASC"
                     /gene_synonym="NEDCPMD; NF; NRCAML"
                     /inference="alignment:Splign:2.1.0"
     exon            866..1036
                     /gene="NFASC"
                     /gene_synonym="NEDCPMD; NF; NRCAML"
                     /inference="alignment:Splign:2.1.0"
     exon            1037..1148
                     /gene="NFASC"
                     /gene_synonym="NEDCPMD; NF; NRCAML"
                     /inference="alignment:Splign:2.1.0"
     exon            1149..1333
                     /gene="NFASC"
                     /gene_synonym="NEDCPMD; NF; NRCAML"
                     /inference="alignment:Splign:2.1.0"
     exon            1334..1465
                     /gene="NFASC"
                     /gene_synonym="NEDCPMD; NF; NRCAML"
                     /inference="alignment:Splign:2.1.0"
     exon            1466..1609
                     /gene="NFASC"
                     /gene_synonym="NEDCPMD; NF; NRCAML"
                     /inference="alignment:Splign:2.1.0"
     exon            1610..1721
                     /gene="NFASC"
                     /gene_synonym="NEDCPMD; NF; NRCAML"
                     /inference="alignment:Splign:2.1.0"
     exon            1722..1888
                     /gene="NFASC"
                     /gene_synonym="NEDCPMD; NF; NRCAML"
                     /inference="alignment:Splign:2.1.0"
     exon            1889..2036
                     /gene="NFASC"
                     /gene_synonym="NEDCPMD; NF; NRCAML"
                     /inference="alignment:Splign:2.1.0"
     exon            2037..2161
                     /gene="NFASC"
                     /gene_synonym="NEDCPMD; NF; NRCAML"
                     /inference="alignment:Splign:2.1.0"
     exon            2162..2206
                     /gene="NFASC"
                     /gene_synonym="NEDCPMD; NF; NRCAML"
                     /inference="alignment:Splign:2.1.0"
     exon            2207..2308
                     /gene="NFASC"
                     /gene_synonym="NEDCPMD; NF; NRCAML"
                     /inference="alignment:Splign:2.1.0"
     exon            2309..2506
                     /gene="NFASC"
                     /gene_synonym="NEDCPMD; NF; NRCAML"
                     /inference="alignment:Splign:2.1.0"
     exon            2507..2577
                     /gene="NFASC"
                     /gene_synonym="NEDCPMD; NF; NRCAML"
                     /inference="alignment:Splign:2.1.0"
     exon            2578..2800
                     /gene="NFASC"
                     /gene_synonym="NEDCPMD; NF; NRCAML"
                     /inference="alignment:Splign:2.1.0"
     exon            2801..2923
                     /gene="NFASC"
                     /gene_synonym="NEDCPMD; NF; NRCAML"
                     /inference="alignment:Splign:2.1.0"
     exon            2924..3097
                     /gene="NFASC"
                     /gene_synonym="NEDCPMD; NF; NRCAML"
                     /inference="alignment:Splign:2.1.0"
     exon            3098..3112
                     /gene="NFASC"
                     /gene_synonym="NEDCPMD; NF; NRCAML"
                     /inference="alignment:Splign:2.1.0"
     exon            3113..3349
                     /gene="NFASC"
                     /gene_synonym="NEDCPMD; NF; NRCAML"
                     /inference="alignment:Splign:2.1.0"
     exon            3350..3466
                     /gene="NFASC"
                     /gene_synonym="NEDCPMD; NF; NRCAML"
                     /inference="alignment:Splign:2.1.0"
     exon            3467..3619
                     /gene="NFASC"
                     /gene_synonym="NEDCPMD; NF; NRCAML"
                     /inference="alignment:Splign:2.1.0"
     exon            3620..3751
                     /gene="NFASC"
                     /gene_synonym="NEDCPMD; NF; NRCAML"
                     /inference="alignment:Splign:2.1.0"
     exon            3752..3821
                     /gene="NFASC"
                     /gene_synonym="NEDCPMD; NF; NRCAML"
                     /inference="alignment:Splign:2.1.0"
     exon            3822..10336
                     /gene="NFASC"
                     /gene_synonym="NEDCPMD; NF; NRCAML"
                     /inference="alignment:Splign:2.1.0"
     regulatory      10315..10320
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NFASC"
                     /gene_synonym="NEDCPMD; NF; NRCAML"
                     /note="hexamer: AATAAA"
     polyA_site      10336
                     /gene="NFASC"
                     /gene_synonym="NEDCPMD; NF; NRCAML"
                     /note="major polyA site"
ORIGIN      
        1 gcgcacgggc tggtctctgc cctaatgcgg cggctggcgg cgagaggcgc tgcaggggac
       61 gcgggggaag tggcggcgcc ggcagcggac agctcggaca gcgcccaggg ccggagcccg
      121 agcccttgga ggttgattga cttatgtgca atttgggacg ctggagttta ccttccctcc
      181 gcagcctgga acagagcctc ctctggtgtt gcaaggaaga ggctgaatga ggcagagaag
      241 ctgagtgctg tccaggaggc ccagttaaag cggctcgagg tgacaagacc ccgagtgctg
      301 gggagcaggg agcagggcca ggtgccgagg atggccaggc agccaccgcc gccctgggtc
      361 catgcagcct tcctcctctg cctcctcagt cttggcggag ccatcgaaat tcctatggat
      421 ccaagcattc agaatgagct gacgcagccg ccaaccatca ccaagcagtc agcgaaggat
      481 cacatcgtgg acccccgtga taacatcctg attgagtgtg aagcaaaagg gaaccctgcc
      541 cccagcttcc actggacacg aaacagcaga ttcttcaaca tcgccaagga cccccgggtg
      601 tccatgagga ggaggtctgg gaccctggtg attgacttcc gcagtggcgg gcggccggag
      661 gaatatgagg gggaatatca gtgcttcgcc cgcaacaaat ttggcacggc cctgtccaat
      721 aggatccgcc tgcaggtgtc taaatctcct ctgtggccca aggaaaacct agaccctgtc
      781 gtggtccaag agggcgctcc tttgacgctc cagtgcaacc ccccgcctgg acttccatcc
      841 ccggtcatct tctggatgag cagctccatg gagcccatca cccaagacaa acgtgtctct
      901 cagggccata acggagacct atacttctcc aacgtgatgc tgcaggacat gcagaccgac
      961 tacagttgta acgcccgctt ccacttcacc cacaccatcc agcagaagaa ccctttcacc
     1021 ctcaaggtcc tcaccacccg aggagttgca gaaagaacac caagcttcat gtatccccag
     1081 ggcaccgcga gcagccagat ggtgcttcgt ggcatggacc tcctgctgga atgcatcgcc
     1141 tccggggtcc caacaccaga catcgcatgg tacaagaaag gtggggacct cccatctgat
     1201 aaggccaagt ttgagaactt taataaggcc ctgcgtatca caaatgtctc tgaggaagac
     1261 tccggggagt atttctgcct ggcctccaac aagatgggca gcatccggca cacgatctcg
     1321 gtgagagtaa aggctgctcc ctactggctg gacgaaccca agaaccttat tctggctcct
     1381 ggcgaggatg ggagactggt gtgtcgagcc aatggaaacc ccaaacccac tgtccagtgg
     1441 atggtgaatg gggaaccttt gcaatcggca ccacctaacc caaaccgtga ggtggccgga
     1501 gacaccatca tcttccggga cacccagatc agcagcaggg ctgtgtacca gtgcaacacc
     1561 tccaacgagc atggctacct gctggccaac gcctttgtca gtgtgctgga tgtgccgcct
     1621 cggatgctgt cgccccggaa ccagctcatt cgagtgattc tttacaaccg gacgcggctg
     1681 gactgccctt tctttgggtc tcccatcccc acactgcgat ggtttaagaa tgggcaagga
     1741 agcaacctgg atggtggcaa ctaccatgtt tatgagaacg gcagtctgga aattaagatg
     1801 atccgcaaag aggaccaggg catctacacc tgtgtcgcca ccaacatcct gggcaaagct
     1861 gaaaaccaag tccgcctgga ggtcaaagac cccaccagga tctaccggat gcccgaggac
     1921 caggtggcca gaaggggcac cacggtgcag ctggagtgtc gggtgaagca cgacccctcc
     1981 ctgaaactca ccgtctcctg gctgaaggat gacgagccgc tctatattgg aaacaggatg
     2041 aagaaggaag acgactccct gaccatcttt ggggtggcag agcgggacca gggcagttac
     2101 acgtgtgtcg ccagcaccga gctagaccaa gacctggcca aggcctacct caccgtgcta
     2161 gctgatcagg ccactccaac taaccgtttg gctgccctgc ccaaaggacg gccagaccgg
     2221 ccccgggacc tggagctgac cgacctggcc gagaggagcg tgcggctgac ctggatcccc
     2281 ggggatgcta acaacagccc catcacagac tacgtcgtcc agtttgaaga agaccagttc
     2341 caacctgggg tctggcatga ccattccaag taccccggca gcgttaactc agccgtcctc
     2401 cggctgtccc cgtatgtcaa ctaccagttc cgtgtcattg ccatcaacga ggttgggagc
     2461 agccacccca gcctcccatc cgagcgctac cgaaccagtg gagcaccccc cgagtccaat
     2521 cctggtgacg tgaagggaga ggggaccaga aagaacaaca tggagatcac gtggacgccc
     2581 atgaatgcca cctcggcctt tggccccaac ctgcgctaca ttgtcaagtg gaggcggaga
     2641 gagactcgag aggcctggaa caacgtcaca gtgtggggct ctcgctacgt ggtggggcag
     2701 accccagtct acgtgcccta tgagatccga gtccaggctg aaaatgactt cgggaagggc
     2761 cctgagccag agtccgtcat cggttactcc ggagaagatt tacccagtgc ccctaggcgt
     2821 ttccgagtcc ggcagcccaa cctggagaca atcaacctgg aatgggatca tcctgagcat
     2881 ccaaatggga tcatgattgg atacactctc aaatatgtgg cctttaacgg gaccaaagta
     2941 ggaaagcaga tagtggaaaa cttctctccc aatcagacca agttcacggt gcaaagaacg
     3001 gaccccgtgt cacgctaccg ctttaccctc agcgccagga cgcaggtggg ctctggggaa
     3061 gccgtcacag aggagtcacc agcacccccg aatgaagcta ctccaaccgc agctcctccc
     3121 acattgcccc cgactaccgt gggtgcgacg ggcgctgtga gcagtaccga tgctactgcc
     3181 attgctgcca ccaccgaagc cacaacagtc cccatcatcc caactgtcgc acctaccacc
     3241 atcgccacca ccaccaccgt cgccacaact actacaacca ctgctgccgc caccaccacc
     3301 acggagagtc ctcccaccac cacctccggg actaagatac acgaatccgc ccctgatgag
     3361 cagtccatat ggaacgtcac ggtgctcccc aacagtaaat gggccaacat cacctggaag
     3421 cacaatttcg ggcccggaac tgactttgtg gttgagtaca tcgacagcaa ccatacgaaa
     3481 aaaactgtcc cagttaaggc ccaggctcag cctatacagc tgacagacct ctatcccggg
     3541 atgacataca cgttgcgggt ttattcccgg gacaacgagg gcatcagcag taccgtcatc
     3601 acctttatga ccagtacagc ttacaccaac aaccaagcgg acatcgccac ccagggctgg
     3661 ttcattgggc ttatgtgcgc catcgccctc ctggtgctga tcctgctcat cgtctgtttc
     3721 atcaagagga gtcgcggcgg caagtaccca gtacgagaaa agaaggatgt tccccttggc
     3781 cctgaagacc ccaaggaaga ggatggctca tttgactata gtgatgagga caacaagccc
     3841 ctgcagggca gtcagacatc tctggacggc accatcaagc agcaggagag tgacgacagc
     3901 ctggtggact atggcgaggg tggcgagggt cagttcaatg aagacggctc cttcatcggc
     3961 cagtacacgg tcaaaaagga caaggaggaa acagagggca acgaaagctc agaggccacg
     4021 tcacctgtca atgctatcta ctctctggcc taacggagcc cacccaggca cagccaccac
     4081 tttgcaagtg ggaggagggg agaaggggag acaaaaccac tgcagaccta ccacgaagcc
     4141 accaccacct tcagtaacaa gggtacgata tgggggtctg ccaagctgtg aggaccagta
     4201 gccaccaagc cacccacaag ccccctccca atgacccccc ttcagccccg ggtgccacca
     4261 gtgtgggaga gctggagccg tggctgagct cagctggagg gagcctggcc ccttgcccgg
     4321 tctcgcagcc accccgagcg ttccaccaca ctgtccgccc ttggcctcgg cacacgctca
     4381 ccttttctgt tggttacggg acttctcatt gtcttaattt cgctttgtgc atcttcccct
     4441 ccagacccaa tgtctctgtt ttcttttcct tttgaaaaag agtccctgga aaagaaagaa
     4501 taagtggatt ctcccccagg agaatccttt ttgcaaagat aggcaaaaag gattgaatcc
     4561 ataccagaac acgtagacag gctgtaatat tcaaaccacc tctgggccaa tgcatttcca
     4621 aaggatgcct ttctcgccat atgcctcccc tggcccccag cccctctgcc tcggccttgt
     4681 cagttgctga gctgggcttg gctcctttct ggaaaatgac agtatttttg gcagggagaa
     4741 ggtgcgcagg cctccttgct gctctctggt ttggttggga ggtgtgttta cctcttgctc
     4801 ctcattcctc ccctgccctt ttctctggaa tatctaagat gtgagctgca ttgactctga
     4861 agacgtttga ggaacaggag tgggcactga tagaaaggac ttcaacgcca gtgactgtgt
     4921 acctccagca gaagaaaatc aggtgtctgg tcttgggggc actgtgctca cttctagaga
     4981 gaagaaaaag gctgggtttg gacttcatgc ctcctcactg gtgaaaccca ggatgtagat
     5041 agagggccac acccaccctg tccagagtag agggcacctg tccacgtggc cagggcccat
     5101 gctgccaccc tcgaggagat ggaaccctgt gatgcaaaag ctttgctggt gtgtttgggg
     5161 cataaggcac tgctcccctt ccatctctag cagatatcat cttctcagcc aattctgtag
     5221 gacactgagg ctcttgtttg acatggtgtg tggatgggga cttgccccaa gactgagcag
     5281 gtctgccctt tcattggggt gtatacatat caggggaccc tgaggtggca ccgtactcag
     5341 tgttgtgatg cccccaccta gggaggactc aatgctcttt gtatgcctta tgcagcgctg
     5401 atctgtccct ggagttccca ggttcccagc tccccccctg caagccttga agcctccagc
     5461 aacgtctatc tccaggagag cagggcagcc tatgcaagtg ttccggcagg accagaagtg
     5521 gccactcaac acacgtcctc accagtcacc ccaaatggag acactcgcag acctgcagtt
     5581 ctcagaccga aggtgctgcc ttcatcctcc ccacctaatg catttttgaa accaaaggta
     5641 cctagcctca gagacagaga aggagagggg gagatcttga gtctaagagg aaccctttag
     5701 ctgtttctgc agctgaggtc agtttcagga gggtgaagct gatccccagg aatggatcag
     5761 ctgccatggg cacagcctcc gatttgagcc tctccggctg ccagccaggg ggcctgggcc
     5821 agaccagggc tctgtccttc cgtgacttta ttaaagcaac atttgccaca tgtttaagcc
     5881 gcaaaggtat tagcaatgct tgcatcatta ataatcattc agttgaaggt caagcccata
     5941 gacacacact ctctgctggt aagaaaccat agcccagaac tgagccaata aggttgtttg
     6001 aacctgagag tgtgtaatgg tcatttgggc caagcctcag ctagggctgc tgcctggacg
     6061 cctgaggggc agcttatcac agtgcaaggg gacagggtaa acagcagggg gtggggtgtg
     6121 agcttggagg gcccctcccc tggatttcct ccaacacctg cttggatact aggcccactg
     6181 tccctgaagg gaatgtcagg gaagtgtgcc tgtgagctga attaaaggac tggcctttcc
     6241 agaaaacgct ttggatctga gcagagtagt cagtttctca gacactcatt ccatactgca
     6301 ccgtacatgc cagcgtaccc attgcaccga aagcaggccc ttagggcagg aagggacgat
     6361 gagcaagtct gccacaccac ctggtgactg ggaagggctg cttctctcgt aagggtcttg
     6421 gggagaagta agcccgcagc ctgcctctgc acctcgttcc agtgtcctgc caggctctgt
     6481 actgtccttt gtgtatgaag aaaatgctgt tagccctctc tcaggctgct gttctgtggg
     6541 tgctgagagc tgccccccat caccacgcca tcacacccct ctgagcagaa gcaaatctga
     6601 ggctgaagaa ttaagttttc cccaagcaag gaaggcaggt ggtcagagag atcaaacgct
     6661 gaggcgtggg gattgcagga ttcaggaggc agcccctttg gggaagagga gggtgcattt
     6721 tccatggagc ccatgtcaga gtccactcca gctcagcagg aaagtggaat tgcccagtac
     6781 tgcctgccag ctgggtctcc caagcaacta ccccaagaca taccaacagt gaagccagga
     6841 cagcaccatg ccatcggcgc tccagtctgt ctggctggga ggaaagtgct gggcatctta
     6901 cagtaactct agaagttcct ttgactaggc tgcaatccca cctatatgct cagccctaag
     6961 agctgtggtt tcctgtgctc tcatttgtcc attcattcat tcattcattc attcagcaaa
     7021 taatctgaaa tccagtctga actaaggcct gagctgtgga taccatggcg cgtgagttag
     7081 atgtggtccc tgccctcatg gggctgacag tcctaaaatc aagtgctagg acacagtggc
     7141 cattgagctg ggagcattct tccctctacc gcttaagcca ctgatggctg cgagcttgaa
     7201 gagagggatt ctgagggaga gcttggtcca tctggtcacc aaggctgggc gggacagagc
     7261 agtcaagaac cacggtgtgg cttgagaaac agtcctgctt cctgagcctc tttgctctgc
     7321 ctcttggggc aagctcctta acctgtctgt atttttatgc ctctgtctcc cattcagttc
     7381 ctccttgaat acctctttct acagagaggc acgggctccc ctgaaggatt gtcgaggcca
     7441 gggttcggga agtacactgc catcggtcag gggacagctg cctcccacct tgcctgggtg
     7501 atggtgaatg ggactcatct tttcctgcct tgcttttgag gagatggttg aatttaagct
     7561 aacaggaaag ccaaaccagc accctccttt gtgctctgag aggttggaga catctggtcc
     7621 accccattgc ccctgcccct ggcaagccct gaagtcggga gaggtgacca caggtctgaa
     7681 aatcagccag tgccaacatt gcttgccctg caaggtgcaa ctgagcccac atagcgcatt
     7741 catagtttcc ctgaccacag ccttgggaag acggtggaaa cccgaggatg tgtgggtttc
     7801 ccagctaacg gacaagctcc cctgccacac ccagtgccca cgcatgctca catacataca
     7861 gcagaccaca accctgttgc caagcagtgc ttccaaggga tagcgatcca gattccaaca
     7921 ttgtcttgga gagggttagt ccacatccaa gttgcgtgag ataagatgca aagggctctg
     7981 tgtggatgag gaacgcacct tagaggagtg ggaaaggcca ccagggttgg gccctgttta
     8041 ggtaattcct gttggcagca cctagagaga gcatctgagc tgaaggagtg ggaaactttg
     8101 cccaagcaat ggcacgggca gcgggctctt tctggcgccc tgtgctggag cagggccaag
     8161 tcttagggca tcacaaacag cccatttgat ggagggagca gggacatagc acatttttgt
     8221 ctgtctttgt gaggctgctt tgctaactct ctgaggagag gaagcctctc gggctttccg
     8281 tcggctgggg ctagtgccag agaatccctt ctcagtggcc agcaggttcc tgggaggccg
     8341 gcacaaggca ccgctcccca ctcatgacac cttggtgcag agtgacctcc tgcccagtca
     8401 ccactccggc cagccccagc caaacacaca aaagcccatg gttgcggttg catctacacc
     8461 gttagttggc aaaggatcct gcttgagctc tgcgtggtgg ccaaggagta gcatggagga
     8521 gggccctgat tttaaaaagg aaaaatagag aggcctcaaa acaatgaaac aaagagcttg
     8581 atatgtcaag aggagaccaa ggccctggga ggcataggca agccgggcag agtcagacca
     8641 gtgccctccc ttgaccatct cctagcattc cttaacctag acaggggcta ccccatgtga
     8701 gtccaagcca gactttgtgg ctgtccccag cctgcacagc ccaagcccag ggaagtgtcc
     8761 tttctttccc ttccttacta ataatgggcc ttcctgagac acattcagag aaggatcaga
     8821 gagaaaggag aaccatccag gagagccaca agcgtccacc aaacagtgtc tcaggcctca
     8881 cctgaagctg ctgttcctcc tatcagcaca ctagtattaa atgggtgttc cataatgagg
     8941 agaatggaaa taggtacaag gcatctagct taggacagaa tcggatttcg gcatgtgaag
     9001 gaatcccaga gctgatctca ttgaaatgat ctatcgtaca gacaaggata tgcaaatcca
     9061 cagaagtgaa gggatttttg ctcaagatca catagctggt aaactaaggt aaggttaggg
     9121 cttgaacttg ggccttctga ctccttgtcc agtcagtgtt ctttcatctc accacagctg
     9181 cctcctttga aacagaggta ttaagatctg tccttctggt tcaccctctc atacctcttt
     9241 actgcgtctc ccacatcccc cacatgcctc ccaaaatgaa agacaaacag gattgtttct
     9301 gagaccaaga tcagtctgtc tgtgatcagc ctgtgtgtgg ttcacccagt catgcaatta
     9361 agggcagatc tgggccagtg gaataggata gctgattggt gtttgttact gtgaacccta
     9421 gaccgtaccc cgtagaatgg tgtctcttgc tttgtaacac atcgggcctt cagtgtgctg
     9481 tattcctcag aagtgagggc atctcggtcc attctgccca tggccacagg gtgcagagag
     9541 gcagcagggc ccatgcaagc tgccaccctg ggatttgctg ggctggagtt caacagatgt
     9601 aaagacttca gtgaagcaat aaacacaaaa ctctgggaga agatatccag aattttgtac
     9661 attactctgt ttctttttca aaaatgaggc agatcagatg cccctgagct gccccttttt
     9721 ttctgattcc caactgcaat gtcctcagtc agtgttgtcc ctctgcccgg ctccccagct
     9781 ctttgccaac ctcttcacac tccccttgag ctgagcatca gtcgcctgtg acgtggccac
     9841 cttctgtcct gctcccactc ccgacccatg ctggaccccg gaggacctcc tgccccgccc
     9901 ccaccacaca cccatatccc ccaccattcc aatttgttct ttcccgtggg gaattttttt
     9961 tcccagcgtc tccatccctt cctacatatc cacacacaca caaattggtc tgatcttttt
    10021 tccattggtt aaacatttaa ctccatgcca gaccttgttt taacccctct cacatcatgt
    10081 tctttccttt tttgcgagtt attttgcatt aaccaacttt gtcagtgaca gatgcgtatc
    10141 tgagggtgtc acacacgacc ttcagcaggg aagacttctg ggccatggag ggccgtctaa
    10201 tacatggact tataaactga ctgcatgagc aatgaaaagg ccaaattatt ctgaattttt
    10261 tttgaatcac tgtaaaaaaa ctgatttctt ttgtatagag aacactaaac gtataataaa
    10321 agttgttcaa aatgga
//
