LOCUS       NP_002490               1461 aa            linear   PRI 27-APR-2025
DEFINITION  neogenin isoform 1 precursor [Homo sapiens].
ACCESSION   NP_002490
VERSION     NP_002490.2
DBSOURCE    REFSEQ: accession NM_002499.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 1461)
  AUTHORS   Vennela,J., Pottakkat,B., Vairappan,B.S., Verma,S.K. and
            Mukherjee,V.
  TITLE     Hepatic Expression of NTN4 and Its Receptors in Patients with
            Hepatocellular Carcinoma
  JOURNAL   Asian Pac J Cancer Prev 24 (12), 4285-4292 (2023)
   PUBMED   38156865
  REMARK    GeneRIF: Hepatic Expression of NTN4 and Its Receptors in Patients
            with Hepatocellular Carcinoma.
            Publication Status: Online-Only
REFERENCE   2  (residues 1 to 1461)
  AUTHORS   Untiveros,G., Raskind,A., Linares,L., Dotti,A. and Strizzi,L.
  TITLE     Netrin-1 Stimulates Migration of Neogenin Expressing Aggressive
            Melanoma Cells
  JOURNAL   Int J Mol Sci 23 (21), 12751 (2022)
   PUBMED   36361539
  REMARK    GeneRIF: Netrin-1 Stimulates Migration of Neogenin Expressing
            Aggressive Melanoma Cells.
            Publication Status: Online-Only
REFERENCE   3  (residues 1 to 1461)
  AUTHORS   Sesen,J., Driscoll,J., Shah,N., Moses-Gardner,A., Luiselli,G.,
            Alexandrescu,S., Zurakowski,D., Baxter,P.A., Su,J.M., Pricola
            Fehnel,K. and Smith,E.R.
  TITLE     Neogenin is highly expressed in diffuse intrinsic pontine glioma
            and influences tumor invasion
  JOURNAL   Brain Res 1762, 147348 (2021)
   PUBMED   33571520
  REMARK    GeneRIF: Neogenin is highly expressed in diffuse intrinsic pontine
            glioma and influences tumor invasion.
REFERENCE   4  (residues 1 to 1461)
  AUTHORS   Sun,D., Tan,Z.B., Sun,X.D., Liu,Z.P., Chen,W.B., Milibari,L.,
            Ren,X., Yao,L.L., Lee,D., Shen,C., Pan,J.X., Huang,Z.H., Mei,L. and
            Xiong,W.C.
  TITLE     Hippocampal astrocytic neogenin regulating glutamate uptake, a
            critical pathway for preventing epileptic response
  JOURNAL   Proc Natl Acad Sci U S A 118 (16) (2021)
   PUBMED   33850017
  REMARK    GeneRIF: Hippocampal astrocytic neogenin regulating glutamate
            uptake, a critical pathway for preventing epileptic response.
REFERENCE   5  (residues 1 to 1461)
  AUTHORS   Huyghe,A., Furlan,G., Ozmadenci,D., Galonska,C., Charlton,J.,
            Gaume,X., Combemorel,N., Riemenschneider,C., Allegre,N., Zhang,J.,
            Wajda,P., Rama,N., Vieugue,P., Durand,I., Brevet,M., Gadot,N.,
            Imhof,T., Merrill,B.J., Koch,M., Mehlen,P., Chazaud,C., Meissner,A.
            and Lavial,F.
  TITLE     Netrin-1 promotes naive pluripotency through Neo1 and Unc5b
            co-regulation of Wnt and MAPK signalling
  JOURNAL   Nat Cell Biol 22 (4), 389-400 (2020)
   PUBMED   32231305
  REMARK    GeneRIF: Netrin-1 promotes naive pluripotency through Neo1 and
            Unc5b co-regulation of Wnt and MAPK signaling.
REFERENCE   6  (residues 1 to 1461)
  AUTHORS   Niederkofler,V., Salie,R. and Arber,S.
  TITLE     Hemojuvelin is essential for dietary iron sensing, and its mutation
            leads to severe iron overload
  JOURNAL   J Clin Invest 115 (8), 2180-2186 (2005)
   PUBMED   16075058
REFERENCE   7  (residues 1 to 1461)
  AUTHORS   Krauss,R.S., Cole,F., Gaio,U., Takaesu,G., Zhang,W. and Kang,J.S.
  TITLE     Close encounters: regulation of vertebrate skeletal myogenesis by
            cell-cell contact
  JOURNAL   J Cell Sci 118 (Pt 11), 2355-2362 (2005)
   PUBMED   15923648
  REMARK    Review article
REFERENCE   8  (residues 1 to 1461)
  AUTHORS   Ho,S.M., Lau,K.M., Mok,S.C. and Syed,V.
  TITLE     Profiling follicle stimulating hormone-induced gene expression
            changes in normal and malignant human ovarian surface epithelial
            cells
  JOURNAL   Oncogene 22 (27), 4243-4256 (2003)
   PUBMED   12833147
  REMARK    GeneRIF: neogenin and restin have roles in
            proproliferation/survival action on ovarian cancer cells
REFERENCE   9  (residues 1 to 1461)
  AUTHORS   Vielmetter,J., Chen,X.N., Miskevich,F., Lane,R.P., Yamakawa,K.,
            Korenberg,J.R. and Dreyer,W.J.
  TITLE     Molecular characterization of human neogenin, a DCC-related
            protein, and the mapping of its gene (NEO1) to chromosomal position
            15q22.3-q23
  JOURNAL   Genomics 41 (3), 414-421 (1997)
   PUBMED   9169140
REFERENCE   10 (residues 1 to 1461)
  AUTHORS   Meyerhardt,J.A., Look,A.T., Bigner,S.H. and Fearon,E.R.
  TITLE     Identification and characterization of neogenin, a DCC-related gene
  JOURNAL   Oncogene 14 (10), 1129-1136 (1997)
   PUBMED   9121761
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AC129980.6, DB458571.2,
            BC117161.1, AB209412.1 and AL355708.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Sep 18, 2007 this sequence version replaced NP_002490.1.
            
            Summary: This gene encodes a cell surface protein that is a member
            of the immunoglobulin superfamily. The encoded protein consists of
            four N-terminal immunoglobulin-like domains, six fibronectin type
            III domains, a transmembrane domain and a C-terminal internal
            domain that shares homology with the tumor suppressor candidate
            gene DCC. This protein may be involved in cell growth and
            differentiation and in cell-cell adhesion. Defects in this gene are
            associated with cell proliferation in certain cancers. Alternate
            splicing results in multiple transcript variants. [provided by
            RefSeq, Feb 2010].
            
            Transcript Variant: This variant (1) represents the longest
            transcript and encodes the longest isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: U61262.1, U72391.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1968540, SAMEA1968968
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000261908.11/ ENSP00000261908.6
            RefSeq Select criteria :: based on conservation
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="15"
                     /map="15q24.1"
     Protein         1..1461
                     /product="neogenin isoform 1 precursor"
                     /note="immunoglobulin superfamily DCC subclass member 2;
                     neogenin homolog 1"
                     /calculated_mol_wt=156446
     sig_peptide     1..33
                     /inference="COORDINATES: ab initio prediction:SignalP:6.0"
                     /calculated_mol_wt=3589
     mat_peptide     34..1461
                     /product="neogenin isoform 1"
                     /calculated_mol_wt=156446
     Region          52..148
                     /region_name="IgI_1_Neogenin_like"
                     /note="First immunoglobulin (Ig)-like domain in neogenin,
                     and similar domains; member of the I-set of Ig superfamily
                     (IgSF) domains; cd05722"
                     /db_xref="CDD:409387"
     Region          52..55
                     /region_name="Ig strand A"
                     /note="Ig strand A [structural motif]"
                     /db_xref="CDD:409387"
     Region          58..64
                     /region_name="Ig strand A'"
                     /note="Ig strand A' [structural motif]"
                     /db_xref="CDD:409387"
     Region          68..78
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409387"
     Site            73
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q92859.2)"
     Region          82..88
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409387"
     Region          90..93
                     /region_name="Ig strand C'"
                     /note="Ig strand C' [structural motif]"
                     /db_xref="CDD:409387"
     Region          100..105
                     /region_name="Ig strand D"
                     /note="Ig strand D [structural motif]"
                     /db_xref="CDD:409387"
     Region          107..113
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409387"
     Region          125..132
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409387"
     Region          137..148
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409387"
     Region          158..239
                     /region_name="IG_like"
                     /note="Immunoglobulin like; smart00410"
                     /db_xref="CDD:214653"
     Region          169..173
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          182..186
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          204..208
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Site            210
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000269|PubMed:16335952,
                     ECO:0000269|PubMed:19349973; propagated from
                     UniProtKB/Swiss-Prot (Q92859.2)"
     Region          218..223
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Region          250..337
                     /region_name="I-set"
                     /note="Immunoglobulin I-set domain; pfam07679"
                     /db_xref="CDD:400151"
     Region          266..270
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409353"
     Region          279..283
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409353"
     Region          303..307
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409353"
     Region          317..322
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409353"
     Site            326
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q92859.2)"
     Region          330..333
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409353"
     Region          344..427
                     /region_name="IgI_4_Neogenin_like"
                     /note="Fourth immunoglobulin (Ig)-like domain in neogenin,
                     and similar domains; member of the I-set of Ig superfamily
                     (IgSF) domains; cd05723"
                     /db_xref="CDD:409388"
     Region          344..347
                     /region_name="Ig strand A"
                     /note="Ig strand A [structural motif]"
                     /db_xref="CDD:409388"
     Region          349..353
                     /region_name="Ig strand A'"
                     /note="Ig strand A' [structural motif]"
                     /db_xref="CDD:409388"
     Region          356..365
                     /region_name="Ig strand B"
                     /note="Ig strand B [structural motif]"
                     /db_xref="CDD:409388"
     Region          370..376
                     /region_name="Ig strand C"
                     /note="Ig strand C [structural motif]"
                     /db_xref="CDD:409388"
     Region          378..381
                     /region_name="Ig strand C'"
                     /note="Ig strand C' [structural motif]"
                     /db_xref="CDD:409388"
     Region          386..390
                     /region_name="Ig strand D"
                     /note="Ig strand D [structural motif]"
                     /db_xref="CDD:409388"
     Region          394..398
                     /region_name="Ig strand E"
                     /note="Ig strand E [structural motif]"
                     /db_xref="CDD:409388"
     Region          406..414
                     /region_name="Ig strand F"
                     /note="Ig strand F [structural motif]"
                     /db_xref="CDD:409388"
     Region          417..427
                     /region_name="Ig strand G"
                     /note="Ig strand G [structural motif]"
                     /db_xref="CDD:409388"
     Region          439..532
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(439,505,520)
                     /site_type="active"
                     /note="Interdomain contacts [active]"
                     /db_xref="CDD:238020"
     Site            470
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000269|PubMed:19349973; propagated from
                     UniProtKB/Swiss-Prot (Q92859.2)"
     Site            489
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000269|PubMed:16335952; propagated from
                     UniProtKB/Swiss-Prot (Q92859.2)"
     Region          <502..924
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain [General function
                     prediction only]; COG3401"
                     /db_xref="CDD:442628"
     Site            order(521..522,524..525)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Site            639
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000269|PubMed:19349973; propagated from
                     UniProtKB/Swiss-Prot (Q92859.2)"
     Site            715
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q92859.2)"
     Site            909
                     /site_type="glycosylation"
                     /note="N-linked (GlcNAc...) asparagine.
                     /evidence=ECO:0000255; propagated from
                     UniProtKB/Swiss-Prot (Q92859.2)"
     Region          956..1051
                     /region_name="FN3"
                     /note="Fibronectin type 3 domain; One of three types of
                     internal repeats found in the plasma protein fibronectin.
                     Its tenth fibronectin type III repeat contains an RGD cell
                     recognition sequence in a flexible loop between 2 strands.
                     Approximately 2% of all...; cd00063"
                     /db_xref="CDD:238020"
     Site            order(1040..1041,1043..1044)
                     /site_type="active"
                     /note="Cytokine receptor motif [active]"
                     /db_xref="CDD:238020"
     Region          1041..1097
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q92859.2)"
     Site            1106..1126
                     /site_type="transmembrane region"
                     /note="propagated from UniProtKB/Swiss-Prot (Q92859.2)"
     Region          1138..1160
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q92859.2)"
     Region          1158..1461
                     /region_name="Neogenin_C"
                     /note="Neogenin C-terminus; pfam06583"
                     /db_xref="CDD:461954"
     Region          1174..1206
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q92859.2)"
     Site            1178
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P97798; propagated from
                     UniProtKB/Swiss-Prot (Q92859.2)"
     Site            1194
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:24275569; propagated from
                     UniProtKB/Swiss-Prot (Q92859.2)"
     Site            1198
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0007744|PubMed:23186163; propagated from
                     UniProtKB/Swiss-Prot (Q92859.2)"
     Region          1235..1276
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q92859.2)"
     Region          1289..1381
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (Q92859.2)"
     Site            1401
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:23186163; propagated from
                     UniProtKB/Swiss-Prot (Q92859.2)"
     Site            1404
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0007744|PubMed:23186163; propagated from
                     UniProtKB/Swiss-Prot (Q92859.2)"
     Site            1432
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P97798; propagated from
                     UniProtKB/Swiss-Prot (Q92859.2)"
     Site            1434
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:23186163; propagated from
                     UniProtKB/Swiss-Prot (Q92859.2)"
     Site            1435
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0000250|UniProtKB:P97798; propagated from
                     UniProtKB/Swiss-Prot (Q92859.2)"
     CDS             1..1461
                     /gene="NEO1"
                     /gene_synonym="IGDCC2; NGN; NTN1R2"
                     /coded_by="NM_002499.4:214..4599"
                     /note="isoform 1 precursor is encoded by transcript
                     variant 1"
                     /db_xref="CCDS:CCDS10247.1"
                     /db_xref="GeneID:4756"
                     /db_xref="HGNC:HGNC:7754"
                     /db_xref="MIM:601907"
ORIGIN      
        1 maaergarrl lstpsfwlyc llllgrrapg aaaarsgsap qspgasirtf tpfyflvepv
       61 dtlsvrgssv ilncsaysep spkiewkkdg tflnlvsddr rqllpdgslf isnvvhskhn
      121 kpdegyyqcv atveslgtii srtaklivag lprftsqpep ssvyagnnai lncevnadlv
      181 pfvrweqnrq plllddrvik lpsgmlvisn ategdgglyr cvvesggppk ysdevelkvl
      241 pdpevisdlv flkqpsplvr vigqdvvlpc vasglptpti kwmkneeald tesserlvll
      301 aggsleisdv teddagtyfc iadngnetie aqaeltvqaq peflkqptni yahesmdivf
      361 ecevtgkptp tvkwvkngdm vipsdyfkiv kehnlqvlgl vksdegfyqc iaendvgnaq
      421 agaqliileh apattgplps aprdvvaslv strfikltwr tpasdphgdn ltysvfytke
      481 giarervent shpgemqvti qnlmpatvyi frvmaqnkhg sgessaplrv etqpevqlpg
      541 papnlrayaa sptsitvtwe tpvsgngeiq nyklyymekg tdkeqdvdvs shsytinglk
      601 kyteysfrvv aynkhgpgvs tpdvavrtls dvpsaapqnl slevrnsksi mihwqppapa
      661 tqngqitgyk iryrkasrks dvtetlvsgt qlsqliegld rgteynfrva altingtgpa
      721 tdwlsaetfe sdldetrvpe vpsslhvrpl vtsivvswtp penqnivvrg yaigygigsp
      781 haqtikvdyk qryytienld psshyvitlk afnnvgegip lyesavtrph tdtsevdlfv
      841 inapytpvpd ptpmmppvgv qasilshdti ritwadnslp khqkitdsry ytvrwktnip
      901 antkyknana ttlsylvtgl kpntlyefsv mvtkgrrsst wsmtahgttf elvptsppkd
      961 vtvvskegkp ktiivnwqpp seangkitgy iiyystdvna eihdwviepv vgnrlthqiq
     1021 eltldtpyyf kiqarnskgm gpmseavqfr tpkadssdkm pndqasgsgg kgsrlpdlgs
     1081 dykppmsgsn sphgsptspl dsnmllviiv svgvitivvv viiavfctrr ttshqkkkra
     1141 acksvngshk ykgnskdvkp pdlwihherl elkpidkspd pnpimtdtpi prnsqditpv
     1201 dnsmdsnihq rrnsyrghes edsmstlagr rgmrpkmmmp fdsqppqpvi sahpihsldn
     1261 phhhfhsssl asparshlyh pgspwpigts mslsdranst esvrntpstd tmpasssqtc
     1321 ctdhqdpega tsssylassq eedsgqslpt ahvrpshplk sfavpaippp gpptydpalp
     1381 stpllsqqal nhhihsvkta sigtlgrsrp pmpvvvpsap evqettrmle dsessyepde
     1441 ltkemahleg lmkdlnaitt a
//
