LOCUS       NM_004713               5819 bp    mRNA    linear   PRI 04-APR-2024
DEFINITION  Homo sapiens nuclear export mediator factor (NEMF), transcript
            variant 1, mRNA.
ACCESSION   NM_004713
VERSION     NM_004713.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 5819)
  AUTHORS   Thrun,A., Garzia,A., Kigoshi-Tansho,Y., Patil,P.R., Umbaugh,C.S.,
            Dallinger,T., Liu,J., Kreger,S., Patrizi,A., Cox,G.A., Tuschl,T.
            and Joazeiro,C.A.P.
  TITLE     Convergence of mammalian RQC and C-end rule proteolytic pathways
            via alanine tailing
  JOURNAL   Mol Cell 81 (10), 2112-2122 (2021)
   PUBMED   33909987
REFERENCE   2  (bases 1 to 5819)
  AUTHORS   Ahmed,A., Wang,M., Bergant,G., Maroofian,R., Zhao,R., Alfadhel,M.,
            Nashabat,M., AlRifai,M.T., Eyaid,W., Alswaid,A., Beetz,C., Qin,Y.,
            Zhu,T., Tian,Q., Xia,L., Wu,H., Shen,L., Dong,S., Yang,X., Liu,C.,
            Ma,L., Zhang,Q., Khan,R., Shah,A.A., Guo,J., Tang,B., Leonardis,L.,
            Writzl,K., Peterlin,B., Guo,H., Malik,S., Xia,K. and Hu,Z.
  TITLE     Biallelic loss-of-function variants in NEMF cause central nervous
            system impairment and axonal polyneuropathy
  JOURNAL   Hum Genet 140 (4), 579-592 (2021)
   PUBMED   33048237
  REMARK    GeneRIF: Biallelic loss-of-function variants in NEMF cause central
            nervous system impairment and axonal polyneuropathy.
REFERENCE   3  (bases 1 to 5819)
  AUTHORS   Udagawa,T., Seki,M., Okuyama,T., Adachi,S., Natsume,T., Noguchi,T.,
            Matsuzawa,A. and Inada,T.
  TITLE     Failure to Degrade CAT-Tailed Proteins Disrupts Neuronal
            Morphogenesis and Cell Survival
  JOURNAL   Cell Rep 34 (1), 108599 (2021)
   PUBMED   33406423
REFERENCE   4  (bases 1 to 5819)
  AUTHORS   Shao,S., Brown,A., Santhanam,B. and Hegde,R.S.
  TITLE     Structure and assembly pathway of the ribosome quality control
            complex
  JOURNAL   Mol Cell 57 (3), 433-444 (2015)
   PUBMED   25578875
  REMARK    GeneRIF: Results show that Listerin specificity for nascent
            chain-60S complexes depends on nuclear export mediator factor.
REFERENCE   5  (bases 1 to 5819)
  AUTHORS   Wang,A.G., Yoon,S.Y., Oh,J.H., Jeon,Y.J., Kim,M., Kim,J.M.,
            Byun,S.S., Yang,J.O., Kim,J.H., Kim,D.G., Yeom,Y.I., Yoo,H.S.,
            Kim,Y.S. and Kim,N.S.
  TITLE     Identification of intrahepatic cholangiocarcinoma related genes by
            comparison with normal liver tissues using expressed sequence tags
  JOURNAL   Biochem Biophys Res Commun 345 (3), 1022-1032 (2006)
   PUBMED   16712791
REFERENCE   6  (bases 1 to 5819)
  AUTHORS   Bi,X., Jones,T., Abbasi,F., Lee,H., Stultz,B., Hursh,D.A. and
            Mortin,M.A.
  TITLE     Drosophila caliban, a nuclear export mediator, can function as a
            tumor suppressor in human lung cancer cells
  JOURNAL   Oncogene 24 (56), 8229-8239 (2005)
   PUBMED   16103875
REFERENCE   7  (bases 1 to 5819)
  AUTHORS   Oh,J.H., Yang,J.O., Hahn,Y., Kim,M.R., Byun,S.S., Jeon,Y.J.,
            Kim,J.M., Song,K.S., Noh,S.M., Kim,S., Yoo,H.S., Kim,Y.S. and
            Kim,N.S.
  TITLE     Transcriptome analysis of human gastric cancer
  JOURNAL   Mamm Genome 16 (12), 942-954 (2005)
   PUBMED   16341674
REFERENCE   8  (bases 1 to 5819)
  AUTHORS   Carbonnelle,D., Liehr,T., Jacquot,C., Masson,D., Lustenberger,P.,
            Denis,M.G. and Roussakis,C.
  TITLE     Assignment of the serologically defined colon cancer antigen 1 gene
            (SDCCAG1) to human chromosome band 14q22 by in situ hybridization
  JOURNAL   Cytogenet Cell Genet 86 (3-4), 248-249 (1999)
   PUBMED   10575219
REFERENCE   9  (bases 1 to 5819)
  AUTHORS   Scanlan,M.J., Chen,Y.T., Williamson,B., Gure,A.O., Stockert,E.,
            Gordan,J.D., Tureci,O., Sahin,U., Pfreundschuh,M. and Old,L.J.
  TITLE     Characterization of human colon cancer antigens recognized by
            autologous antibodies
  JOURNAL   Int J Cancer 76 (5), 652-658 (1998)
   PUBMED   9610721
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL627171.1, AL591767.2 and
            KF459555.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_004713.5.
            
            Summary: This gene encodes a component of the ribosome quality
            control complex. The encoded protein facilitates the recognition
            and ubiquitination of stalled 60S subunits by the ubiquitin ligase
            listerin. A similar protein in fly functions as a tumor suppressor.
            [provided by RefSeq, Jul 2016].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660809.85475.1,
                                           SRR1803611.109743.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000298310.10/ ENSP00000298310.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-94                AL627171.1         110001-110094       c
            95-163              AL627171.1         109113-109181       c
            164-266             AL627171.1         108869-108971       c
            267-392             AL627171.1         103446-103571       c
            393-541             AL627171.1         98024-98172         c
            542-609             AL627171.1         95445-95512         c
            610-696             AL627171.1         91669-91755         c
            697-770             AL627171.1         90729-90802         c
            771-841             AL627171.1         89513-89583         c
            842-917             AL627171.1         89357-89432         c
            918-980             AL627171.1         88605-88667         c
            981-1058            AL627171.1         86655-86732         c
            1059-1267           AL627171.1         86360-86568         c
            1268-1459           AL627171.1         85922-86113         c
            1460-1523           AL627171.1         85597-85660         c
            1524-1612           AL627171.1         83173-83261         c
            1613-1716           AL627171.1         72060-72163         c
            1717-1779           AL627171.1         71294-71356         c
            1780-1818           AL627171.1         63401-63439         c
            1819-1892           AL591767.2         187362-187435       c
            1893-1950           AL591767.2         184578-184635       c
            1951-2009           AL591767.2         184010-184068       c
            2010-2130           AL591767.2         183794-183914       c
            2131-2407           AL591767.2         181761-182037       c
            2408-2450           AL591767.2         180977-181019       c
            2451-2500           AL591767.2         180816-180865       c
            2501-2654           AL591767.2         177132-177285       c
            2655-2732           AL591767.2         170837-170914       c
            2733-2930           AL591767.2         170487-170684       c
            2931-2963           AL591767.2         168059-168091       c
            2964-3064           AL591767.2         166561-166661       c
            3065-3108           AL591767.2         166433-166476       c
            3109-3188           AL591767.2         166266-166345       c
            3189-3442           KF459555.1         1-254               c
            3443-5819           AL591767.2         163424-165800       c
FEATURES             Location/Qualifiers
     source          1..5819
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q21.3"
     gene            1..5819
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /note="nuclear export mediator factor"
                     /db_xref="GeneID:9147"
                     /db_xref="HGNC:HGNC:10663"
                     /db_xref="MIM:608378"
     exon            1..94
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /inference="alignment:Splign:2.1.0"
     CDS             36..3266
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /note="isoform 1 is encoded by transcript variant 1;
                     serologically defined colon cancer antigen 1; antigen
                     NY-CO-1; caliban homolog; ribosome quality control complex
                     subunit NEMF; New-York Colon 1"
                     /codon_start=1
                     /product="ribosome quality control complex subunit NEMF
                     isoform 1"
                     /protein_id="NP_004704.3"
                     /db_xref="CCDS:CCDS9694.1"
                     /db_xref="GeneID:9147"
                     /db_xref="HGNC:HGNC:10663"
                     /db_xref="MIM:608378"
                     /translation="MKSRFSTIDLRAVLAELNASLLGMRVNNVYDVDNKTYLIRLQKP
                     DFKATLLLESGIRIHTTEFEWPKNMMPSSFAMKCRKHLKSRRLVSAKQLGVDRIVDFQ
                     FGSDEAAYHLIIELYDRGNIVLTDYEYVILNILRFRTDEADDVKFAVRERYPLDHARA
                     AEPLLTLERLTEIVASAPKGELLKRVLNPLLPYGPALIEHCLLENGFSGNVKVDEKLE
                     TKDIEKVLVSLQKAEDYMKTTSNFSGKGYIIQKREIKPSLEADKPVEDILTYEEFHPF
                     LFSQHSQCPYIEFESFDKAVDEFYSKIEGQKIDLKALQQEKQALKKLDNVRKDHENRL
                     EALQQAQEIDKLKGELIEMNLQIVDRAIQVVRSALANQIDWTEIGLIVKEAQAQGDPV
                     ASAIKELKLQTNHVTMLLRNPYLLSEEEDDDVDGDVNVEKNETEPPKGKKKKQKNKQL
                     QKPQKNKPLLVDVDLSLSAYANAKKYYDHKRYAAKKTQKTVEAAEKAFKSAEKKTKQT
                     LKEVQTVTSIQKARKVYWFEKFLWFISSENYLIIGGRDQQQNEIIVKRYLTPGDIYVH
                     ADLHGATSCVIKNPTGEPIPPRTLTEAGTMALCYSAAWDARVITSAWWVYHHQVSKTA
                     PTGEYLTTGSFMIRGKKNFLPPSYLMMGFSFLFKVDESCVWRHQGERKVRVQDEDMET
                     LASCTSELISEEMEQLDGGDTSSDEDKEEHETPVEVELMTQVDQEDITLQSGRDELNE
                     ELIQEESSEDEGEYEEVRKDQDSVGEMKDEGEETLNYPDTTIDLSHLQPQRSIQKLAS
                     KEESSNSSDSKSQSRRHLSAKERREMKKKKLPSDSGDLEALEGKDKEKESTVHIETHQ
                     NTSKNVAAVQPMKRGQKSKMKKMKEKYKDQDEEDRELIMKLLGSAGSNKEEKGKKGKK
                     GKTKDEPVKKQPQKPRGGQRVSDNIKKETPFLEVITHELQDFAVDDPHDDKEEQDLDQ
                     QGNEENLFDSLTGQPHPEDVLLFAIPICAPYTTMTNYKYKVKLTPGVQKKGKAAKTAL
                     NSFMHSKEATAREKDLFRSVKDTDLSRNIPGKVKVSAPNLLNVKRK"
     exon            95..163
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            164..266
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            267..392
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            393..541
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            542..609
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            610..696
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            697..770
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            771..841
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            842..917
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            918..980
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            981..1058
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1059..1267
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1268..1459
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1460..1523
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1524..1612
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1613..1716
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1717..1779
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1780..1892
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1893..1950
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            1951..2009
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            2010..2130
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            2131..2407
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            2408..2450
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            2451..2500
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            2501..2654
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            2655..2732
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            2733..2930
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            2931..2963
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            2964..3064
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            3065..3108
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            3109..3188
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /inference="alignment:Splign:2.1.0"
     exon            3189..5819
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      4066..4071
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /note="hexamer: AATATA"
     regulatory      4075..4080
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /note="hexamer: AATAAT"
     polyA_site      4088
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /note="major polyA site"
     polyA_site      4098
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
     regulatory      4589..4594
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /note="hexamer: ATTAAA"
     polyA_site      4624
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
     regulatory      5802..5807
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /note="hexamer: AATAAA"
     polyA_site      5819
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
ORIGIN      
        1 agaggaactt gcggtagtga ccctcgggcc tcgccatgaa gagccgcttt agcaccattg
       61 acctccgcgc cgtactcgcg gagctgaatg ctagcttgct aggaatgaga gtaaacaatg
      121 tttatgatgt ggataataag acatacctta ttcgtcttca aaaaccggac tttaaagcta
      181 cacttttact tgaatctggc atacgaattc atacaacaga atttgagtgg cctaagaata
      241 tgatgccgtc tagttttgcc atgaagtgcc gaaaacattt gaagagtcgg agattagtca
      301 gtgcaaaaca gcttggtgtg gatagaattg tagattttca atttggaagt gatgaagctg
      361 cttaccattt aatcattgag ctctatgata gggggaacat tgttcttaca gattatgagt
      421 acgtaatttt aaatattcta aggtttcgaa ctgatgaggc agatgatgtt aaatttgctg
      481 ttcgtgaacg ctatccactt gatcatgcta gagctgctga acctttgctt actttggaaa
      541 ggttgactga aatagtagcc agcgcaccta agggtgaact actgaagagg gtgcttaacc
      601 cattacttcc ctatggacca gctctcattg aacactgtct tttagaaaat ggattctcgg
      661 gtaatgtcaa agtggatgaa aaacttgaaa ctaaagatat tgaaaaagta cttgtttctc
      721 tgcagaaagc agaagactat atgaaaacaa catccaactt cagtgggaag ggatatatca
      781 ttcagaaaag agaaataaaa ccaagcttgg aagcagataa accagttgaa gacatactga
      841 cgtatgagga atttcatcct ttcttgtttt ctcaacattc acaatgtcca tatatagaat
      901 ttgaatcatt tgacaaggcg gtggatgaat tttattccaa gatagaaggc cagaaaattg
      961 acttaaaagc tttacaacag gaaaagcaag cattgaagaa attagataat gttcgaaagg
     1021 atcacgaaaa cagattggaa gctcttcagc aggctcagga aatagacaaa ctgaaaggag
     1081 agctcataga aatgaaccta caaatagttg acagagccat tcaggtagtt cgaagtgctt
     1141 tagctaacca gatagattgg acagaaattg ggttaattgt gaaagaagcc caggctcaag
     1201 gagaccctgt tgcaagtgca atcaaagaat taaaactaca aacaaaccat gttacaatgc
     1261 tgctaagaaa tccatacttg ttatcagagg aggaagatga tgatgttgat ggtgacgtca
     1321 atgttgagaa aaatgaaact gaaccaccaa aaggaaaaaa gaaaaaacaa aagaataaac
     1381 agctgcagaa gcctcagaaa aataagccct tacttgtaga tgttgatctc agcttgtcag
     1441 catatgccaa tgccaaaaag tattatgatc acaagagata tgctgctaag aaaacacaaa
     1501 agactgttga agctgctgag aaggcattca agtcagcaga aaagaaaaca aagcaaacat
     1561 taaaagaagt tcagactgtt acctctattc aaaaagcaag aaaagtatat tggtttgaga
     1621 aatttctgtg gttcattagc tcagagaact atctaattat aggtggacga gatcagcaac
     1681 agaatgaaat aattgtgaaa agatacttga caccaggaga catttatgta catgctgatc
     1741 ttcatggagc tactagctgt gtaattaaga atccaacagg agaacccatc cccccacgga
     1801 ccttgactga agctggcaca atggcacttt gctacagtgc tgcttgggat gcacgagtta
     1861 tcactagtgc ttggtgggtg taccatcatc aggtatctaa aacagcacca actggagaat
     1921 atttgacaac aggaagcttc atgataagag gaaaaaagaa ttttcttcct ccctcatatc
     1981 taatgatggg gtttagcttc ctttttaagg tagatgagtc ttgtgtttgg agacatcagg
     2041 gtgaacgaaa agtcagagta caggatgaag acatggagac actggcaagt tgtacaagtg
     2101 aactcatatc agaagaaatg gaacaattag atggaggtga cacgagcagt gatgaggata
     2161 aagaagaaca tgaaactcct gtggaagtag aactcatgac tcaggttgac caagaggata
     2221 tcactcttca gagtggcaga gatgaactaa atgaggagct cattcaggaa gaaagctctg
     2281 aagacgaagg agaatatgaa gaggttagaa aagatcagga ttctgttggt gaaatgaagg
     2341 atgaagggga agagacatta aattatcctg atactaccat tgacttgtct caccttcaac
     2401 cccaaaggtc catccagaaa ttggcttcaa aagaggaatc ttctaattct agtgacagta
     2461 aatcacagag ccggagacat ttgtcagcca aggaaagaag ggaaatgaaa aagaaaaaac
     2521 ttccaagtga ctcaggagat ttagaagcgt tagagggaaa ggataaagaa aaagaaagta
     2581 ctgtacacat tgaaactcat cagaacacaa gcaaaaatgt tgcggctgtg cagccaatga
     2641 aacgaggaca aaagagtaaa atgaaaaaaa tgaaagaaaa atacaaagac caggatgaag
     2701 aagaccgtga acttatcatg aagttgctgg ggtctgcagg ttcaaacaaa gaagaaaaag
     2761 ggaagaaggg gaagaaagga aaaacaaagg acgaacctgt gaagaaacag ccccagaaac
     2821 ctagaggtgg acagagggtc tctgacaaca ttaagaaaga aactccgttc cttgaggtta
     2881 taactcatga gttacaagac tttgctgtag atgatccaca tgatgacaag gaagagcaag
     2941 atctggatca acagggaaat gaggaaaacc tatttgattc tttgacaggc cagccacatc
     3001 ctgaagatgt actactgttt gccattccaa tatgtgcccc ttacaccacc atgacaaact
     3061 acaaatataa agtgaaactt actcctggag tgcagaaaaa gggaaaagct gcaaaaacag
     3121 ccttgaatag tttcatgcat tccaaagaag caacagcaag agaaaaagac ttattccgca
     3181 gcgtaaagga cacagattta tcaagaaaca ttcctggcaa agtgaaagtg tctgcaccca
     3241 atcttctgaa cgtaaaaagg aaatagctga aatgaaattc taaaatattt gagaagagcc
     3301 aattttatag ccttttggaa gttcaaagat gaaagcacca tgtatcagga tttccgcatt
     3361 ataaaaatga actaaacatt gccttgctat attcaccaaa aggacttact tcttgttttt
     3421 tcccagtttt atatagagga aacactgtct atgataggat ttccaaaagt atttgtggac
     3481 agttaaatgc taattaatat acatctgtag ttattctaca ttttcttgaa atttgggagg
     3541 ttaataccaa gtattcattt catgatgtaa agaaactgaa cagtgaagtg gcttgattgc
     3601 ttaaactatt gacttggtaa gtctactgta tataacatct aatatatata ttacaggcca
     3661 aatgaactaa acattgcctt gctatattca ccaaaaggac ttaattcttg tttttttccc
     3721 agttttatat agaggaaaca ctatgatagg atttcctaaa gtatttgtgg acagttaaat
     3781 gctaattata tacatctgta gttattctac attttcttga aatttgggag gttaatacca
     3841 agtattcatt tcatgatgta aagaaactga acagtgaagt ggcttgattg cttaaactat
     3901 tgacttggta agtctactgt atataacatc taatatatat atattatagg ccagctacaa
     3961 ggggtttaaa tatttaggat tgtgtcttga aaactaagta ttggagtgga ttttcttctg
     4021 ctttcattga tacttgtcag aaaaaaatat tagaccaaaa tgtaaaatat aagtaataat
     4081 tctcatgact cagcttaatc atttctattc atttgtagac cttcaggttt gatcttaatt
     4141 ttttccttgt aatctgattc agttctgtac cattttaatt tccaccttta gtaattaatt
     4201 accaatatag cacgttagag tatcttaacc tataatacca gaagcgcatt ttaggcttac
     4261 cactaaagac tctaacccac ttttcctctg tctcttaaca gaatggaaaa tgatgtcatc
     4321 attatttcca ttccttcatg agctataaaa cctattaatt aaaaaaaaac tttttaattg
     4381 actggacagc acctgcctgt attcagcatg accacttgct ccagccccta cactgaagca
     4441 ttaaggtcaa agtactgaac agttgttggt ccctccacac tttgtttttc cagcttcgtc
     4501 ataatgtgaa tattaatgtt taaaaaaaaa aacctttatt atattgtatt tacaacacta
     4561 ggttacataa ctatagacta ctcttgccat taaaaaaaaa aaaatctata atgttttcaa
     4621 aaaatgactt cagcattttg atgatggtaa gactataatg tttctacaga tacatatcct
     4681 gaagttactg aaaagaaaat gtagcaacca ttcttcaggg taaaaagccc aaccattact
     4741 ttactttaat agaggacagc tactggtgtt aaatacattt attgtaaact ttagacacaa
     4801 aaataggttc tctaggccat tcacatgcac attaaaacca acaggtgcaa actacaacaa
     4861 tgcatataat tatacaaatg atgccactct gtgatgttta caggattgct gtccatgcaa
     4921 ggtgatcata ggcattattt atgaagcctt aagatccaga agtgttgtta ctaccaaacc
     4981 tctgattaac actgtgaagt aagtgttttg gaaggcagtt ccatgagttg gctaacattt
     5041 ctttaaagca aatgactgct tctaagctta gcctgaaagt ggacaaggaa agagaaaagt
     5101 aatttcactt tactgtttgg aagtacattc acttgaaaaa ctacataaaa catccctctt
     5161 tctttcttca aagattttca taggaactgg ttatagcaat gaagttttat tgcccttaaa
     5221 taatccataa acctagtttt tcgagagtgc taagtcttag gaaacacaat tttaaataat
     5281 ctaagtgcca agtacaaagt tacttaccgt acaagagatt ttggttgaac tgaaaatatt
     5341 agtatttcat tactgtgtgc ctgaaaaata aataggaaga acacatttaa aagcaaatgc
     5401 tttgcaagta agtttcagta ataatgtgaa ggtagtatac ttacagctct gtgatggaca
     5461 agcaagttgt tggcacatcc accaaaaaca attacttctc cttcatcgct ggcacaagct
     5521 gtgtgccata acctgcattt aaaacaaaaa cgagtttgaa gccacccaga acaccagttg
     5581 gccattttat actacagaat agagctatga aataaagact agcgatctta aggtagttca
     5641 gaccaaaaca tcatggcgag aagtaaaatc ttaagggaaa gtagcaaaag cagatcaatc
     5701 aatatctatt ttatctggat attattttct ctctctggtc gttctcattt atgcaatgag
     5761 gagtttgaac tgaacgatct gttctctagg tctttgttag caataaaaat cgtatggga
//
