LOCUS       NP_004704               1076 aa            linear   PRI 12-JUN-2025
DEFINITION  ribosome quality control complex subunit NEMF isoform 1 [Homo
            sapiens].
ACCESSION   NP_004704
VERSION     NP_004704.3
DBSOURCE    REFSEQ: accession NM_004713.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (residues 1 to 1076)
  AUTHORS   Thrun,A., Garzia,A., Kigoshi-Tansho,Y., Patil,P.R., Umbaugh,C.S.,
            Dallinger,T., Liu,J., Kreger,S., Patrizi,A., Cox,G.A., Tuschl,T.
            and Joazeiro,C.A.P.
  TITLE     Convergence of mammalian RQC and C-end rule proteolytic pathways
            via alanine tailing
  JOURNAL   Mol Cell 81 (10), 2112-2122 (2021)
   PUBMED   33909987
REFERENCE   2  (residues 1 to 1076)
  AUTHORS   Ahmed,A., Wang,M., Bergant,G., Maroofian,R., Zhao,R., Alfadhel,M.,
            Nashabat,M., AlRifai,M.T., Eyaid,W., Alswaid,A., Beetz,C., Qin,Y.,
            Zhu,T., Tian,Q., Xia,L., Wu,H., Shen,L., Dong,S., Yang,X., Liu,C.,
            Ma,L., Zhang,Q., Khan,R., Shah,A.A., Guo,J., Tang,B., Leonardis,L.,
            Writzl,K., Peterlin,B., Guo,H., Malik,S., Xia,K. and Hu,Z.
  TITLE     Biallelic loss-of-function variants in NEMF cause central nervous
            system impairment and axonal polyneuropathy
  JOURNAL   Hum Genet 140 (4), 579-592 (2021)
   PUBMED   33048237
  REMARK    GeneRIF: Biallelic loss-of-function variants in NEMF cause central
            nervous system impairment and axonal polyneuropathy.
REFERENCE   3  (residues 1 to 1076)
  AUTHORS   Udagawa,T., Seki,M., Okuyama,T., Adachi,S., Natsume,T., Noguchi,T.,
            Matsuzawa,A. and Inada,T.
  TITLE     Failure to Degrade CAT-Tailed Proteins Disrupts Neuronal
            Morphogenesis and Cell Survival
  JOURNAL   Cell Rep 34 (1), 108599 (2021)
   PUBMED   33406423
REFERENCE   4  (residues 1 to 1076)
  AUTHORS   Yip,M.C.J., Savickas,S., Gygi,S.P. and Shao,S.
  TITLE     ELAC1 Repairs tRNAs Cleaved during Ribosome-Associated Quality
            Control
  JOURNAL   Cell Rep 30 (7), 2106-2114 (2020)
   PUBMED   32075755
REFERENCE   5  (residues 1 to 1076)
  AUTHORS   Shao,S., Brown,A., Santhanam,B. and Hegde,R.S.
  TITLE     Structure and assembly pathway of the ribosome quality control
            complex
  JOURNAL   Mol Cell 57 (3), 433-444 (2015)
   PUBMED   25578875
  REMARK    GeneRIF: Results show that Listerin specificity for nascent
            chain-60S complexes depends on nuclear export mediator factor.
REFERENCE   6  (residues 1 to 1076)
  AUTHORS   Wang,A.G., Yoon,S.Y., Oh,J.H., Jeon,Y.J., Kim,M., Kim,J.M.,
            Byun,S.S., Yang,J.O., Kim,J.H., Kim,D.G., Yeom,Y.I., Yoo,H.S.,
            Kim,Y.S. and Kim,N.S.
  TITLE     Identification of intrahepatic cholangiocarcinoma related genes by
            comparison with normal liver tissues using expressed sequence tags
  JOURNAL   Biochem Biophys Res Commun 345 (3), 1022-1032 (2006)
   PUBMED   16712791
REFERENCE   7  (residues 1 to 1076)
  AUTHORS   Bi,X., Jones,T., Abbasi,F., Lee,H., Stultz,B., Hursh,D.A. and
            Mortin,M.A.
  TITLE     Drosophila caliban, a nuclear export mediator, can function as a
            tumor suppressor in human lung cancer cells
  JOURNAL   Oncogene 24 (56), 8229-8239 (2005)
   PUBMED   16103875
REFERENCE   8  (residues 1 to 1076)
  AUTHORS   Oh,J.H., Yang,J.O., Hahn,Y., Kim,M.R., Byun,S.S., Jeon,Y.J.,
            Kim,J.M., Song,K.S., Noh,S.M., Kim,S., Yoo,H.S., Kim,Y.S. and
            Kim,N.S.
  TITLE     Transcriptome analysis of human gastric cancer
  JOURNAL   Mamm Genome 16 (12), 942-954 (2005)
   PUBMED   16341674
REFERENCE   9  (residues 1 to 1076)
  AUTHORS   Carbonnelle,D., Liehr,T., Jacquot,C., Masson,D., Lustenberger,P.,
            Denis,M.G. and Roussakis,C.
  TITLE     Assignment of the serologically defined colon cancer antigen 1 gene
            (SDCCAG1) to human chromosome band 14q22 by in situ hybridization
  JOURNAL   Cytogenet Cell Genet 86 (3-4), 248-249 (1999)
   PUBMED   10575219
REFERENCE   10 (residues 1 to 1076)
  AUTHORS   Scanlan,M.J., Chen,Y.T., Williamson,B., Gure,A.O., Stockert,E.,
            Gordan,J.D., Tureci,O., Sahin,U., Pfreundschuh,M. and Old,L.J.
  TITLE     Characterization of human colon cancer antigens recognized by
            autologous antibodies
  JOURNAL   Int J Cancer 76 (5), 652-658 (1998)
   PUBMED   9610721
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL627171.1, AL591767.2 and
            KF459555.1.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NP_004704.2.
            
            Summary: This gene encodes a component of the ribosome quality
            control complex. The encoded protein facilitates the recognition
            and ubiquitination of stalled 60S subunits by the ubiquitin ligase
            listerin. A similar protein in fly functions as a tumor suppressor.
            [provided by RefSeq, Jul 2016].
            
            Transcript Variant: This variant (1) represents the longer
            transcript and encodes the longer isoform (1).
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR1660809.85475.1,
                                           SRR1803611.109743.1 [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000298310.10/ ENSP00000298310.5
            RefSeq Select criteria :: based on conservation, expression,
                                      longest protein
            ##RefSeq-Attributes-END##
FEATURES             Location/Qualifiers
     source          1..1076
                     /organism="Homo sapiens"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q21.3"
     Protein         1..1076
                     /product="ribosome quality control complex subunit NEMF
                     isoform 1"
                     /note="serologically defined colon cancer antigen 1;
                     antigen NY-CO-1; caliban homolog; ribosome quality control
                     complex subunit NEMF; New-York Colon 1"
                     /calculated_mol_wt=122824
     Region          6..644
                     /region_name="RqcH_arch"
                     /note="ribosome rescue protein RqcH, archaeal type;
                     NF041120"
                     /db_xref="CDD:469043"
     Site            7
                     /site_type="phosphorylation"
                     /note="Phosphothreonine.
                     /evidence=ECO:0007744|PubMed:23186163; propagated from
                     UniProtKB/Swiss-Prot (O60524.4)"
     Site            417
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:18669648,
                     ECO:0007744|PubMed:19690332, ECO:0007744|PubMed:20068231,
                     ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:23186163,
                     ECO:0007744|PubMed:24275569; propagated from
                     UniProtKB/Swiss-Prot (O60524.4)"
     Region          420..453
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (O60524.4)"
     Region          <658..776
                     /region_name="COG5137"
                     /note="Histone chaperone involved in gene silencing
                     [Transcription / Chromatin structure and dynamics]"
                     /db_xref="CDD:227466"
     Region          691..715
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (O60524.4)"
     Region          742..972
                     /region_name="Disordered.
                     /evidence=ECO:0000256|SAM:MobiDB-lite"
                     /note="propagated from UniProtKB/Swiss-Prot (O60524.4)"
     Site            747
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:18669648,
                     ECO:0007744|PubMed:21406692; propagated from
                     UniProtKB/Swiss-Prot (O60524.4)"
     Site            748
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:18669648,
                     ECO:0007744|PubMed:21406692; propagated from
                     UniProtKB/Swiss-Prot (O60524.4)"
     Site            763
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:18669648; propagated from
                     UniProtKB/Swiss-Prot (O60524.4)"
     Site            831
                     /site_type="phosphorylation"
                     /note="Phosphoserine.
                     /evidence=ECO:0007744|PubMed:20068231,
                     ECO:0007744|PubMed:23186163; propagated from
                     UniProtKB/Swiss-Prot (O60524.4)"
     Region          975..1066
                     /region_name="NFACT-C"
                     /note="NFACT protein C-terminal domain; pfam11923"
                     /db_xref="CDD:432191"
     CDS             1..1076
                     /gene="NEMF"
                     /gene_synonym="IDDSAPN; NY-CO-1; RQC2; SDCCAG1"
                     /coded_by="NM_004713.6:36..3266"
                     /note="isoform 1 is encoded by transcript variant 1"
                     /db_xref="CCDS:CCDS9694.1"
                     /db_xref="GeneID:9147"
                     /db_xref="HGNC:HGNC:10663"
                     /db_xref="MIM:608378"
ORIGIN      
        1 mksrfstidl ravlaelnas llgmrvnnvy dvdnktylir lqkpdfkatl llesgiriht
       61 tefewpknmm pssfamkcrk hlksrrlvsa kqlgvdrivd fqfgsdeaay hliielydrg
      121 nivltdyeyv ilnilrfrtd eaddvkfavr erypldhara aeplltlerl teivasapkg
      181 ellkrvlnpl lpygpalieh cllengfsgn vkvdekletk diekvlvslq kaedymktts
      241 nfsgkgyiiq kreikpslea dkpvedilty eefhpflfsq hsqcpyiefe sfdkavdefy
      301 skiegqkidl kalqqekqal kkldnvrkdh enrlealqqa qeidklkgel iemnlqivdr
      361 aiqvvrsala nqidwteigl ivkeaqaqgd pvasaikelk lqtnhvtmll rnpyllseee
      421 dddvdgdvnv ekneteppkg kkkkqknkql qkpqknkpll vdvdlslsay anakkyydhk
      481 ryaakktqkt veaaekafks aekktkqtlk evqtvtsiqk arkvywfekf lwfissenyl
      541 iiggrdqqqn eiivkryltp gdiyvhadlh gatscviknp tgepipprtl teagtmalcy
      601 saawdarvit sawwvyhhqv sktaptgeyl ttgsfmirgk knflppsylm mgfsflfkvd
      661 escvwrhqge rkvrvqdedm etlasctsel iseemeqldg gdtssdedke ehetpvevel
      721 mtqvdqedit lqsgrdelne eliqeessed egeyeevrkd qdsvgemkde geetlnypdt
      781 tidlshlqpq rsiqklaske essnssdsks qsrrhlsake rremkkkklp sdsgdleale
      841 gkdkekestv hiethqntsk nvaavqpmkr gqkskmkkmk ekykdqdeed relimkllgs
      901 agsnkeekgk kgkkgktkde pvkkqpqkpr ggqrvsdnik ketpflevit helqdfavdd
      961 phddkeeqdl dqqgneenlf dsltgqphpe dvllfaipic apyttmtnyk ykvkltpgvq
     1021 kkgkaaktal nsfmhskeat arekdlfrsv kdtdlsrnip gkvkvsapnl lnvkrk
//
