LOCUS       NM_001145108            3553 bp    mRNA    linear   PRI 21-SEP-2024
DEFINITION  Homo sapiens neural EGFL like 2 (NELL2), transcript variant 3,
            mRNA.
ACCESSION   NM_001145108
VERSION     NM_001145108.2
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 3553)
  AUTHORS   Miyaguchi,M., Nakanishi,Y., Maturana,A.D., Mizutani,K. and Niimi,T.
  TITLE     Conformational Change of the Hairpin-like-structured Robo2
            Ectodomain Allows NELL1/2 Binding
  JOURNAL   J Mol Biol 434 (19), 167777 (2022)
   PUBMED   35940226
  REMARK    GeneRIF: Conformational Change of the Hairpin-like-structured Robo2
            Ectodomain Allows NELL1/2 Binding.
REFERENCE   2  (bases 1 to 3553)
  AUTHORS   Liu,J., Liu,D., Zhang,X., Li,Y., Fu,X., He,W., Li,M., Chen,P.,
            Zeng,G., DiSanto,M.E., Wang,X. and Zhang,X.
  TITLE     NELL2 modulates cell proliferation and apoptosis via ERK pathway in
            the development of benign prostatic hyperplasia
  JOURNAL   Clin Sci (Lond) 135 (13), 1591-1608 (2021)
   PUBMED   34195782
  REMARK    GeneRIF: NELL2 modulates cell proliferation and apoptosis via ERK
            pathway in the development of benign prostatic hyperplasia.
REFERENCE   3  (bases 1 to 3553)
  AUTHORS   Pan,R., Lu,Q., Ren,C., Li,H., Zeng,F., Tian,X. and Chen,H.
  TITLE     Anoctamin 5 promotes osteosarcoma development by increasing
            degradation of Nel-like proteins 1 and 2
  JOURNAL   Aging (Albany NY) 13 (13), 17316-17327 (2021)
   PUBMED   34238763
  REMARK    GeneRIF: Anoctamin 5 promotes osteosarcoma development by
            increasing degradation of Nel-like proteins 1 and 2.
REFERENCE   4  (bases 1 to 3553)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 3553)
  AUTHORS   Kiyozumi,D., Noda,T., Yamaguchi,R., Tobita,T., Matsumura,T.,
            Shimada,K., Kodani,M., Kohda,T., Fujihara,Y., Ozawa,M., Yu,Z.,
            Miklossy,G., Bohren,K.M., Horie,M., Okabe,M., Matzuk,M.M. and
            Ikawa,M.
  TITLE     NELL2-mediated lumicrine signaling through OVCH2 is required for
            male fertility
  JOURNAL   Science 368 (6495), 1132-1135 (2020)
   PUBMED   32499443
REFERENCE   6  (bases 1 to 3553)
  AUTHORS   Zhang,Z. and Henzel,W.J.
  TITLE     Signal peptide prediction based on analysis of experimentally
            verified cleavage sites
  JOURNAL   Protein Sci 13 (10), 2819-2824 (2004)
   PUBMED   15340161
REFERENCE   7  (bases 1 to 3553)
  AUTHORS   Kuroda,S., Oyasu,M., Kawakami,M., Kanayama,N., Tanizawa,K.,
            Saito,N., Abe,T., Matsuhashi,S. and Ting,K.
  TITLE     Biochemical characterization and expression analysis of neural
            thrombospondin-1-like proteins NELL1 and NELL2
  JOURNAL   Biochem Biophys Res Commun 265 (1), 79-86 (1999)
   PUBMED   10548494
REFERENCE   8  (bases 1 to 3553)
  AUTHORS   Luce,M.J. and Burrows,P.D.
  TITLE     The neuronal EGF-related genes NELL1 and NELL2 are expressed in
            hemopoietic cells and developmentally regulated in the B lineage
  JOURNAL   Gene 231 (1-2), 121-126 (1999)
   PUBMED   10231576
REFERENCE   9  (bases 1 to 3553)
  AUTHORS   Beckmann,G., Hanke,J., Bork,P. and Reich,J.G.
  TITLE     Merging extracellular domains: fold prediction for laminin G-like
            and amino-terminal thrombospondin-like modules based on homology to
            pentraxins
  JOURNAL   J Mol Biol 275 (5), 725-730 (1998)
   PUBMED   9480764
REFERENCE   10 (bases 1 to 3553)
  AUTHORS   Watanabe,T.K., Katagiri,T., Suzuki,M., Shimizu,F., Fujiwara,T.,
            Kanemoto,N., Nakamura,Y., Hirai,Y., Maekawa,H. and Takahashi,Ei.
  TITLE     Cloning and characterization of two novel human cDNAs (NELL1 and
            NELL2) encoding proteins with six EGF-like repeats
  JOURNAL   Genomics 38 (3), 273-276 (1996)
   PUBMED   8975702
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from DA307194.1, AL527608.3,
            BC020544.1 and DB316660.1.
            
            On Feb 27, 2020 this sequence version replaced NM_001145108.1.
            
            Summary: The protein encoded by this gene is a glycoprotein
            containing several von Willebrand factor C domains and epidermal
            growth factor (EGF)-like domains. The encoded protein acts as a
            homotrimer and is found in the cytoplasm. Several variants encoding
            a few different isoforms exist, and at least one isoform appears to
            be a secreted protein. Studies in mouse suggest that this protein
            plays a role in neural cell growth and differentiation as well as
            in oncogenesis. [provided by RefSeq, Feb 2009].
            
            Transcript Variant: This variant (3) differs in the 5' UTR and
            coding sequence compared to variant 1. The resulting isoform (b) is
            shorter at the N-terminus compared to isoform a. Variants 2 and 3
            both encode isoform b.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC020544.1, SRR14038197.2379814.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000429094.7/ ENSP00000390680.2
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-22                DA307194.1         151-172
            23-144              AL527608.3         1-122
            145-3349            BC020544.1         1-3205
            3350-3553           DB316660.1         4-207               c
FEATURES             Location/Qualifiers
     source          1..3553
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="12"
                     /map="12q12"
     gene            1..3553
                     /gene="NELL2"
                     /gene_synonym="NRP2"
                     /note="neural EGFL like 2"
                     /db_xref="GeneID:4753"
                     /db_xref="HGNC:HGNC:7751"
                     /db_xref="MIM:602320"
     exon            1..501
                     /gene="NELL2"
                     /gene_synonym="NRP2"
                     /inference="alignment:Splign:2.1.0"
     CDS             447..2897
                     /gene="NELL2"
                     /gene_synonym="NRP2"
                     /note="isoform b precursor is encoded by transcript
                     variant 3; neural epidermal growth factor-like 2;
                     NEL-related protein 2; NEL-like protein 2; protein kinase
                     C-binding protein NELL2"
                     /codon_start=1
                     /product="protein kinase C-binding protein NELL2 isoform b
                     precursor"
                     /protein_id="NP_001138580.1"
                     /db_xref="CCDS:CCDS8746.1"
                     /db_xref="GeneID:4753"
                     /db_xref="HGNC:HGNC:7751"
                     /db_xref="MIM:602320"
                     /translation="MESRVLLRTFCLIFGLGAVWGLGVDPSLQIDVLTELELGESTTG
                     VRQVPGLHNGTKAFLFQDTPRSIKASTATAEQFFQKLRNKHEFTILVTLKQTHLNSGV
                     ILSIHHLDHRYLELESSGHRNEVRLHYRSGSHRPHTEVFPYILADDKWHKLSLAISAS
                     HLILHIDCNKIYERVVEKPSTDLPLGTTFWLGQRNNAHGYFKGIMQDVQLLVMPQGFI
                     AQCPDLNRTCPTCNDFHGLVQKIMELQDILAKTSAKLSRAEQRMNRLDQCYCERTCTM
                     KGTTYREFESWIDGCKNCTCLNGTIQCETLICPNPDCPLKSALAYVDGKCCKECKSIC
                     QFQGRTYFEGERNTVYSSSGVCVLYECKDQTMKLVESSGCPALDCPESHQITLSHSCC
                     KVCKGYDFCSERHNCMENSICRNLNDRAVCSCRDGFRALREDNAYCEDIDECAEGRHY
                     CRENTMCVNTPGSFMCICKTGYIRIDDYSCTEHDECITNQHNCDENALCFNTVGGHNC
                     VCKPGYTGNGTTCKAFCKDGCRNGGACIAANVCACPQGFTGPSCETDIDECSDGFVQC
                     DSRANCINLPGWYHCECRDGYHDNGMFSPSGESCEDIDECGTGRHSCANDTICFNLDG
                     GYDCRCPHGKNCTGDCIHDGKVKHNGQIWVLENDRCSVCSCQNGFVMCRRMVCDCENP
                     TVDLFCCPECDPRLSSQCLHQNGETLYNSGDTWVQNCQQCRCLQGEVDCWPLPCPDVE
                     CEFSILPENECCPRCVTDPCQADTIRNDITKTCLDEMNVVRFTGSSWIKHGTECTLCQ
                     CKNGHICCSVDPQCLQEL"
     exon            502..630
                     /gene="NELL2"
                     /gene_synonym="NRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            631..781
                     /gene="NELL2"
                     /gene_synonym="NRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            782..955
                     /gene="NELL2"
                     /gene_synonym="NRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            956..1052
                     /gene="NELL2"
                     /gene_synonym="NRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1053..1125
                     /gene="NELL2"
                     /gene_synonym="NRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1126..1208
                     /gene="NELL2"
                     /gene_synonym="NRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1209..1337
                     /gene="NELL2"
                     /gene_synonym="NRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1338..1440
                     /gene="NELL2"
                     /gene_synonym="NRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1441..1532
                     /gene="NELL2"
                     /gene_synonym="NRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1533..1635
                     /gene="NELL2"
                     /gene_synonym="NRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1636..1764
                     /gene="NELL2"
                     /gene_synonym="NRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1765..1890
                     /gene="NELL2"
                     /gene_synonym="NRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            1891..2013
                     /gene="NELL2"
                     /gene_synonym="NRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2014..2109
                     /gene="NELL2"
                     /gene_synonym="NRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2110..2250
                     /gene="NELL2"
                     /gene_synonym="NRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2251..2444
                     /gene="NELL2"
                     /gene_synonym="NRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2445..2621
                     /gene="NELL2"
                     /gene_synonym="NRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2622..2846
                     /gene="NELL2"
                     /gene_synonym="NRP2"
                     /inference="alignment:Splign:2.1.0"
     exon            2847..3553
                     /gene="NELL2"
                     /gene_synonym="NRP2"
                     /inference="alignment:Splign:2.1.0"
     regulatory      3529..3534
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NELL2"
                     /gene_synonym="NRP2"
                     /note="hexamer: AATAAA"
     polyA_site      3553
                     /gene="NELL2"
                     /gene_synonym="NRP2"
                     /note="major polyA site"
ORIGIN      
        1 attgtctctg cctttacaac aggttcgggc ggcggggaag acgggggggt gcggggccgc
       61 cccagcccgg gctttcttgg ggccgccccc cttctaccgg gtgtgcgagt ctttggctgc
      121 ttttattcgg ctcgggagct aattccccga cggagccgcg ccggggcgag tccgacccct
      181 ccctccgggc cccctccggg ccgcgctgcc gcctcggccc tgcgtgtggg aatgatgtgc
      241 gcattggagg gtctaagttc ttcacgcgcc tggggaggcc tcccttttct ttcttaggca
      301 accaaagcgt attaatccta ctgatcagta aatccgaggc agcagcagga gagacaaacg
      361 ttattttccc gcttgattcc aagaacctct tcgattttta tttttatttt taaagaggga
      421 gacgatggac tgagctgatc cgcaccatgg agtctcgggt cttactgaga acattctgtt
      481 tgatcttcgg tctcggagca gtttgggggc ttggtgtgga cccttcccta cagattgacg
      541 tcttaacaga gttagaactt ggggagtcca cgaccggagt gcgtcaggtc ccggggctgc
      601 ataatgggac gaaagccttt ctctttcaag atactcccag aagcataaaa gcatccactg
      661 ctacagctga acagtttttt cagaagctga gaaataaaca tgaatttact attttggtga
      721 ccctaaaaca gacccactta aattcaggag ttattctctc aattcaccac ttggatcaca
      781 ggtacctgga actggaaagt agtggccatc ggaatgaagt cagactgcat taccgctcag
      841 gcagtcaccg ccctcacaca gaagtgtttc cttacatttt ggctgatgac aagtggcaca
      901 agctctcctt agccatcagt gcttcccatt tgattttaca cattgactgc aataaaattt
      961 atgaaagggt agtagaaaag ccctccacag acttgcctct aggcacaaca ttttggctag
     1021 gacagagaaa taatgcgcat ggatatttta agggtataat gcaagatgtc caattacttg
     1081 tcatgcccca gggatttatt gctcagtgcc cagatcttaa tcgcacctgt ccaacttgca
     1141 atgacttcca tggacttgtg cagaaaatca tggagctaca ggatatttta gccaaaacat
     1201 cagccaagct gtctcgagct gaacagcgaa tgaatagatt ggatcagtgc tattgtgaaa
     1261 ggacttgcac catgaaggga accacctacc gagaatttga gtcctggata gacggctgta
     1321 agaactgcac atgcctgaat ggaaccatcc agtgtgaaac tctaatctgc ccaaatcctg
     1381 actgcccact taagtcggct cttgcgtatg tggatggcaa atgctgtaag gaatgcaaat
     1441 cgatatgcca atttcaagga cgaacctact ttgaaggaga aagaaataca gtctattcct
     1501 cttctggagt atgtgttctc tatgagtgca aggaccagac catgaaactt gttgagagtt
     1561 caggctgtcc agctttggat tgtccagagt ctcatcagat aaccttgtct cacagctgtt
     1621 gcaaagtttg taaaggttat gacttttgtt ctgaaaggca taactgcatg gagaattcca
     1681 tctgcagaaa tctgaatgac agggctgttt gtagctgtcg agatggtttt agggctcttc
     1741 gagaggataa tgcctactgt gaagacatcg atgagtgtgc tgaagggcgc cattactgtc
     1801 gtgaaaatac aatgtgtgtc aacaccccgg gttcttttat gtgcatctgc aaaactggat
     1861 acatcagaat tgatgattat tcatgtacag aacatgatga gtgtatcaca aatcagcaca
     1921 actgtgatga aaatgcttta tgcttcaaca ctgttggagg acacaactgt gtttgcaagc
     1981 cgggctatac agggaatgga acgacatgca aagcattttg caaagatggc tgtaggaatg
     2041 gaggagcctg tattgccgct aatgtgtgtg cctgcccaca aggcttcact ggacccagct
     2101 gtgaaacgga cattgatgaa tgctctgatg gttttgttca atgtgacagt cgtgctaatt
     2161 gcattaacct gcctggatgg taccactgtg agtgcagaga tggctaccat gacaatggga
     2221 tgttttcacc aagtggagaa tcgtgtgaag atattgatga gtgtgggacc gggaggcaca
     2281 gctgtgccaa tgataccatt tgcttcaatt tggatggcgg atatgattgt cgatgtcctc
     2341 atggaaagaa ttgcacaggg gactgcatcc atgatggaaa agttaagcac aatggtcaga
     2401 tttgggtgtt ggaaaatgac aggtgctctg tgtgctcatg tcagaatgga ttcgttatgt
     2461 gtcgacggat ggtctgtgac tgtgagaatc ccacagttga tcttttttgc tgccctgaat
     2521 gtgacccaag gcttagtagt cagtgcctcc atcaaaatgg ggaaactttg tataacagtg
     2581 gtgacacctg ggtccagaat tgtcaacagt gccgctgctt gcaaggggaa gttgattgtt
     2641 ggcccctgcc ttgcccagat gtggagtgtg aattcagcat tctcccagag aatgagtgct
     2701 gcccgcgctg tgtcacagac ccttgccagg ctgacaccat ccgcaatgac atcaccaaga
     2761 cttgcctgga cgaaatgaat gtggttcgct tcaccgggtc ctcttggatc aaacatggca
     2821 ctgagtgtac tctctgccag tgcaagaatg gccacatctg ttgctcagtg gatccacagt
     2881 gccttcagga actgtgaagt taactgtctc atgggagatt tctgttaaaa gaatgttctt
     2941 tcattaaaag accaaaaaga agttaaaact taaattgggt gatttgtggg cagctaaatg
     3001 cagctttgtt aatagctgag tgaactttca attatgaaat ttgtggagct tgacaaaatc
     3061 acaaaaggaa aattactggg gcaaaattag acctcaagtc tgcctctact gtgtctcaca
     3121 tcaccatgta gaagaatggg cgtacagtat ataccgtgac atcctgaacc ctggatagaa
     3181 agcctgagcc cattggatct gtgaaagcct ctagcttcac tggtgcagaa aattttcctc
     3241 tagatcagaa tcttcaagaa tcagttaggt tcctcactgc aagaaataaa atgtcaggca
     3301 gtgaatgaat tatattttca gaagtaaagc aaagaagcta taacatgtta tgtacagtac
     3361 actctgaaaa gaaatctgaa acaagttatt gtaatgataa aaataatgca caggcatggt
     3421 tacttaatat tttctaacag gaaaagtcat ccctatttcc ttgttttact gcacttaata
     3481 ttatttggtt gaatttgttc agtataagct cgttcttgtg caaaattaaa taaatatttc
     3541 tcttacctta taa
//
