LOCUS       NM_198976               2237 bp    mRNA    linear   PRI 20-SEP-2024
DEFINITION  Homo sapiens negative elongation factor complex member C/D
            (NELFCD), mRNA.
ACCESSION   NM_198976
VERSION     NM_198976.4
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Wang,S., Sun,Y., Li,C., Chong,Y., Ai,M., Wang,Y., Shi,H. and
            Shang,Y.
  TITLE     TH1L involvement in colorectal cancer pathogenesis by regulation of
            CCL20 through the NF-kappaB signalling pathway
  JOURNAL   J Cell Mol Med 28 (10), e18391 (2024)
   PUBMED   38809918
  REMARK    GeneRIF: TH1L involvement in colorectal cancer pathogenesis by
            regulation of CCL20 through the NF-kappaB signalling pathway.
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Cao,Y., Qin,Y., Zhang,W., Tian,W., Ren,Y., Ren,J., Wang,J.,
            Wang,M., Jiang,J. and Wang,Z.
  TITLE     Structural basis of the human negative elongation factor NELF-B/C/E
            ternary complex
  JOURNAL   Biochem Biophys Res Commun 677, 155-161 (2023)
   PUBMED   37591184
  REMARK    GeneRIF: Structural basis of the human negative elongation factor
            NELF-B/C/E ternary complex.
REFERENCE   3  (bases 1 to 2237)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 2237)
  AUTHORS   Haenig,C., Atias,N., Taylor,A.K., Mazza,A., Schaefer,M.H., Russ,J.,
            Riechers,S.P., Jain,S., Coughlin,M., Fontaine,J.F., Freibaum,B.D.,
            Brusendorf,L., Zenkner,M., Porras,P., Stroedicke,M., Schnoegl,S.,
            Arnsburg,K., Boeddrich,A., Pigazzini,L., Heutink,P., Taylor,J.P.,
            Kirstein,J., Andrade-Navarro,M.A., Sharan,R. and Wanker,E.E.
  TITLE     Interactome Mapping Provides a Network of Neurodegenerative Disease
            Proteins and Uncovers Widespread Protein Aggregation in Affected
            Brains
  JOURNAL   Cell Rep 32 (7), 108050 (2020)
   PUBMED   32814053
REFERENCE   5  (bases 1 to 2237)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   6  (bases 1 to 2237)
  AUTHORS   Yin,X.L., Chen,S. and Gu,J.X.
  TITLE     Identification of TH1 as an interaction partner of A-Raf kinase
  JOURNAL   Mol Cell Biochem 231 (1-2), 69-74 (2002)
   PUBMED   11952167
  REMARK    GeneRIF: In a two-hybrid screen of human fetal liver cDNA library,
            TH1 was detected as a new interaction partner of A-Raf; this
            specific interaction may have played a critical role in the
            activation of A-Raf.
REFERENCE   7  (bases 1 to 2237)
  AUTHORS   Deloukas,P., Matthews,L.H., Ashurst,J., Burton,J., Gilbert,J.G.,
            Jones,M., Stavrides,G., Almeida,J.P., Babbage,A.K., Bagguley,C.L.,
            Bailey,J., Barlow,K.F., Bates,K.N., Beard,L.M., Beare,D.M.,
            Beasley,O.P., Bird,C.P., Blakey,S.E., Bridgeman,A.M., Brown,A.J.,
            Buck,D., Burrill,W., Butler,A.P., Carder,C., Carter,N.P.,
            Chapman,J.C., Clamp,M., Clark,G., Clark,L.N., Clark,S.Y.,
            Clee,C.M., Clegg,S., Cobley,V.E., Collier,R.E., Connor,R.,
            Corby,N.R., Coulson,A., Coville,G.J., Deadman,R., Dhami,P.,
            Dunn,M., Ellington,A.G., Frankland,J.A., Fraser,A., French,L.,
            Garner,P., Grafham,D.V., Griffiths,C., Griffiths,M.N., Gwilliam,R.,
            Hall,R.E., Hammond,S., Harley,J.L., Heath,P.D., Ho,S., Holden,J.L.,
            Howden,P.J., Huckle,E., Hunt,A.R., Hunt,S.E., Jekosch,K.,
            Johnson,C.M., Johnson,D., Kay,M.P., Kimberley,A.M., King,A.,
            Knights,A., Laird,G.K., Lawlor,S., Lehvaslaiho,M.H., Leversha,M.,
            Lloyd,C., Lloyd,D.M., Lovell,J.D., Marsh,V.L., Martin,S.L.,
            McConnachie,L.J., McLay,K., McMurray,A.A., Milne,S., Mistry,D.,
            Moore,M.J., Mullikin,J.C., Nickerson,T., Oliver,K., Parker,A.,
            Patel,R., Pearce,T.A., Peck,A.I., Phillimore,B.J.,
            Prathalingam,S.R., Plumb,R.W., Ramsay,H., Rice,C.M., Ross,M.T.,
            Scott,C.E., Sehra,H.K., Shownkeen,R., Sims,S., Skuce,C.D.,
            Smith,M.L., Soderlund,C., Steward,C.A., Sulston,J.E., Swann,M.,
            Sycamore,N., Taylor,R., Tee,L., Thomas,D.W., Thorpe,A., Tracey,A.,
            Tromans,A.C., Vaudin,M., Wall,M., Wallis,J.M., Whitehead,S.L.,
            Whittaker,P., Willey,D.L., Williams,L., Williams,S.A., Wilming,L.,
            Wray,P.W., Hubbard,T., Durbin,R.M., Bentley,D.R., Beck,S. and
            Rogers,J.
  TITLE     The DNA sequence and comparative analysis of human chromosome 20
  JOURNAL   Nature 414 (6866), 865-871 (2001)
   PUBMED   11780052
REFERENCE   8  (bases 1 to 2237)
  AUTHORS   Ping,Y.H. and Rana,T.M.
  TITLE     DSIF and NELF interact with RNA polymerase II elongation complex
            and HIV-1 Tat stimulates P-TEFb-mediated phosphorylation of RNA
            polymerase II and DSIF during transcription elongation
  JOURNAL   J Biol Chem 276 (16), 12951-12958 (2001)
   PUBMED   11112772
REFERENCE   9  (bases 1 to 2237)
  AUTHORS   Bonthron,D.T., Hayward,B.E., Moran,V. and Strain,L.
  TITLE     Characterization of TH1 and CTSZ, two non-imprinted genes
            downstream of GNAS1 in chromosome 20q13
  JOURNAL   Hum Genet 107 (2), 165-175 (2000)
   PUBMED   11030415
REFERENCE   10 (bases 1 to 2237)
  AUTHORS   Yamaguchi,Y., Takagi,T., Wada,T., Yano,K., Furuya,A., Sugimoto,S.,
            Hasegawa,J. and Handa,H.
  TITLE     NELF, a multisubunit complex containing RD, cooperates with DSIF to
            repress RNA polymerase II elongation
  JOURNAL   Cell 97 (1), 41-51 (1999)
   PUBMED   10199401
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AK293410.1 and BQ022435.1.
            
            On May 17, 2019 this sequence version replaced NM_198976.3.
            
            Summary: The NELF complex of proteins interacts with the DSIF
            protein complex to repress transcriptional elongation by RNA
            polymerase II. The protein encoded by this gene is an essential
            part of the NELF complex. Alternative translation initiation site
            usage results in the formation of two isoforms with different
            N-termini. [provided by RefSeq, Jul 2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: SRR3476690.1126501.1, BC014952.1
                                           [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            CDS uses downstream in-frame AUG :: upstream AUG and CDS extension
                                                is not conserved
            MANE Ensembl match               :: ENST00000652272.2/
                                                ENSP00000499018.1
            RefSeq Select criteria           :: based on conservation,
                                                expression, longest protein
            ##RefSeq-Attributes-END##
            COMPLETENESS: complete on the 3' end.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-2131              AK293410.1         1-2131
            2132-2237           BQ022435.1         36-141              c
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="20"
                     /map="20q13.32"
     gene            1..2237
                     /gene="NELFCD"
                     /gene_synonym="HSPC130; NELF-C; NELF-D; TH1; TH1L"
                     /note="negative elongation factor complex member C/D"
                     /db_xref="GeneID:51497"
                     /db_xref="HGNC:HGNC:15934"
                     /db_xref="MIM:605297"
     exon            1..114
                     /gene="NELFCD"
                     /gene_synonym="HSPC130; NELF-C; NELF-D; TH1; TH1L"
                     /inference="alignment:Splign:2.1.0"
     CDS             55..1800
                     /gene="NELFCD"
                     /gene_synonym="HSPC130; NELF-C; NELF-D; TH1; TH1L"
                     /note="TH1 drosophila homolog; trihydrophobin 1; negative
                     elongation factor proteins C and D; NELF-C/D; TH1-like
                     protein"
                     /codon_start=1
                     /product="negative elongation factor C/D"
                     /protein_id="NP_945327.3"
                     /db_xref="CCDS:CCDS13473.3"
                     /db_xref="GeneID:51497"
                     /db_xref="HGNC:HGNC:15934"
                     /db_xref="MIM:605297"
                     /translation="MDEDYYGSAAEWGDEADGGQQEDDSGEGEDDAEVQQECLHKFST
                     RDYIMEPSIFNTLKRYFQAGGSPENVIQLLSENYTAVAQTVNLLAEWLIQTGVEPVQV
                     QETVENHLKSLLIKHFDPRKADSIFTEEGETPAWLEQMIAHTTWRDLFYKLAEAHPDC
                     LMLNFTVKLISDAGYQGEITSVSTACQQLEVFSRVLRTSLATILDGGEENLEKNLPEF
                     AKMVCHGEHTYLFAQAMMSVLAQEEQGGSAVRRIAQEVQRFAQEKGHDASQITLALGT
                     AASYPRACQALGAMLSKGALNPADITVLFKMFTSMDPPPVELIRVPAFLDLFMQSLFK
                     PGARINQDHKHKYIHILAYAASVVETWKKNKRVSINKDELKSTSKAVETVHNLCCNEN
                     KGASELVAELSTLYQCIRFPVVAMGVLKWVDWTVSEPRYFQLQTDHTPVHLALLDEIS
                     TCHQLLHPQVLQLLVKLFETEHSQLDVMEQLELKKTLLDRMVHLLSRGYVLPVVSYIR
                     KCLEKLDTDISLIRYFVTEVLDVIAPPYTSDFVQLFLPILENDSIAGTIKTEGEHDPV
                     TEFIAHCKSNFIMVN"
     exon            115..230
                     /gene="NELFCD"
                     /gene_synonym="HSPC130; NELF-C; NELF-D; TH1; TH1L"
                     /inference="alignment:Splign:2.1.0"
     exon            231..340
                     /gene="NELFCD"
                     /gene_synonym="HSPC130; NELF-C; NELF-D; TH1; TH1L"
                     /inference="alignment:Splign:2.1.0"
     exon            341..450
                     /gene="NELFCD"
                     /gene_synonym="HSPC130; NELF-C; NELF-D; TH1; TH1L"
                     /inference="alignment:Splign:2.1.0"
     exon            451..558
                     /gene="NELFCD"
                     /gene_synonym="HSPC130; NELF-C; NELF-D; TH1; TH1L"
                     /inference="alignment:Splign:2.1.0"
     exon            559..711
                     /gene="NELFCD"
                     /gene_synonym="HSPC130; NELF-C; NELF-D; TH1; TH1L"
                     /inference="alignment:Splign:2.1.0"
     exon            712..842
                     /gene="NELFCD"
                     /gene_synonym="HSPC130; NELF-C; NELF-D; TH1; TH1L"
                     /inference="alignment:Splign:2.1.0"
     exon            843..1008
                     /gene="NELFCD"
                     /gene_synonym="HSPC130; NELF-C; NELF-D; TH1; TH1L"
                     /inference="alignment:Splign:2.1.0"
     exon            1009..1143
                     /gene="NELFCD"
                     /gene_synonym="HSPC130; NELF-C; NELF-D; TH1; TH1L"
                     /inference="alignment:Splign:2.1.0"
     exon            1144..1283
                     /gene="NELFCD"
                     /gene_synonym="HSPC130; NELF-C; NELF-D; TH1; TH1L"
                     /inference="alignment:Splign:2.1.0"
     exon            1284..1398
                     /gene="NELFCD"
                     /gene_synonym="HSPC130; NELF-C; NELF-D; TH1; TH1L"
                     /inference="alignment:Splign:2.1.0"
     exon            1399..1494
                     /gene="NELFCD"
                     /gene_synonym="HSPC130; NELF-C; NELF-D; TH1; TH1L"
                     /inference="alignment:Splign:2.1.0"
     exon            1495..1635
                     /gene="NELFCD"
                     /gene_synonym="HSPC130; NELF-C; NELF-D; TH1; TH1L"
                     /inference="alignment:Splign:2.1.0"
     exon            1636..1765
                     /gene="NELFCD"
                     /gene_synonym="HSPC130; NELF-C; NELF-D; TH1; TH1L"
                     /inference="alignment:Splign:2.1.0"
     exon            1766..2237
                     /gene="NELFCD"
                     /gene_synonym="HSPC130; NELF-C; NELF-D; TH1; TH1L"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2219..2224
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NELFCD"
                     /gene_synonym="HSPC130; NELF-C; NELF-D; TH1; TH1L"
                     /note="hexamer: AGTAAA"
     polyA_site      2237
                     /gene="NELFCD"
                     /gene_synonym="HSPC130; NELF-C; NELF-D; TH1; TH1L"
                     /note="major polyA site"
ORIGIN      
        1 atgcgccgcg ctcgctcgcg ggagggcatg gcgggggccg tgccgggcgc catcatggac
       61 gaggactact acgggagcgc ggccgagtgg ggcgacgagg ctgacggcgg ccagcaggag
      121 gatgattctg gagaaggaga ggatgatgcg gaggttcagc aagaatgcct gcataaattt
      181 tccacccggg attatatcat ggaaccctcc atcttcaaca ctctgaagag gtattttcag
      241 gcaggagggt ctccagagaa tgttatccag ctcttatctg aaaactacac cgctgtggcc
      301 cagactgtga acctgctggc cgagtggctc attcagacag gtgttgagcc agtgcaggtt
      361 caggaaactg tggaaaatca cttgaagagt ttgctgatca aacattttga cccccgcaaa
      421 gcagattcta tttttactga agaaggagag accccagcgt ggctggaaca gatgattgca
      481 cataccacgt ggcgggacct tttttataaa ctggctgaag cccatccaga ctgtttgatg
      541 ctgaacttca ccgttaagct tatttctgac gcagggtacc agggggagat caccagtgtg
      601 tccacagcat gccagcagct agaagtgttc tcgagagtgc tccggacctc tctagctaca
      661 attttagatg gaggagaaga aaaccttgaa aaaaatctcc ctgagtttgc caagatggtg
      721 tgccacgggg agcacacgta cctgtttgcc caggccatga tgtccgtgct ggcccaggag
      781 gagcaggggg gctccgctgt gcgcaggatc gcccaggaag tgcagcgctt tgcccaggag
      841 aaaggtcatg acgccagtca gatcacacta gccttgggca cagctgcctc ctaccccagg
      901 gcctgccagg ctctcggggc catgctgtcc aaaggagccc tgaaccctgc tgacatcacc
      961 gtcctgttca agatgttcac aagcatggac cctcctccgg ttgaacttat ccgcgttcca
     1021 gccttcctgg acctgttcat gcagtcactc tttaaaccag gggctcggat caaccaggac
     1081 cacaagcaca aatacatcca catcttggcg tacgcagcaa gcgtggttga gacctggaag
     1141 aagaacaagc gagtgagcat caataaagat gagctgaagt caacgtcaaa agctgtcgaa
     1201 accgttcaca atttgtgttg caacgagaac aaaggggcct ctgaactagt ggcagaattg
     1261 agcacacttt atcagtgtat taggtttcca gtggtagcaa tgggtgtgct gaagtgggtg
     1321 gattggactg tatcagaacc aaggtacttt cagctgcaga ctgaccatac ccctgtccac
     1381 ctggcgttgc tggatgagat cagcacctgc caccagctcc tgcaccccca ggtcctgcag
     1441 ctgcttgtta agctttttga gactgagcac tcccagctgg acgtgatgga gcagcttgag
     1501 ttgaagaaga cactgctgga caggatggtt cacctgctga gtcgaggtta tgtacttcct
     1561 gttgtcagtt acatccgaaa gtgtctggag aagctggaca ctgacatttc actcattcgc
     1621 tattttgtca ctgaggtgct ggacgtcatt gctcctcctt atacctctga cttcgtgcaa
     1681 cttttcctcc ccatcctgga gaatgacagc atcgcaggta ccatcaaaac ggaaggcgag
     1741 catgaccctg tgacggagtt tatagctcac tgcaaatcta acttcatcat ggtgaactaa
     1801 tttagagcat cctccagagc tgaagcagaa cattccagaa cccgttgtgg aaaaaccctt
     1861 tcaagaagct gttttaagag gctcgggcag cgtcttgaaa atgggcaccg ctgggaggag
     1921 gtggatgact tctttacaaa ggaaaatggt agcagcttca gtgagaaact gcccttacaa
     1981 acagtccctt ctctgctgtc aatccaatac tgctcccaaa tcctgttttc agtgttcatt
     2041 tccctcaagg caggcgctgg gctcccacga cccctcagga cagatctggc cgtcagccgc
     2101 gggccgctgg gaactccact cggggaactc ctttccaagc tgacctcagt tttctcacaa
     2161 gaacccagtt agctgatgtt ttattgtaat tgtcttaatt tgctaagaac aagtaataag
     2221 taaattttta aaaagcc
//
