LOCUS       NM_015456               2540 bp    mRNA    linear   PRI 19-SEP-2024
DEFINITION  Homo sapiens negative elongation factor complex member B (NELFB),
            mRNA.
ACCESSION   NM_015456 XM_939381
VERSION     NM_015456.5
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 2540)
  AUTHORS   Cao,Y., Qin,Y., Zhang,W., Tian,W., Ren,Y., Ren,J., Wang,J.,
            Wang,M., Jiang,J. and Wang,Z.
  TITLE     Structural basis of the human negative elongation factor NELF-B/C/E
            ternary complex
  JOURNAL   Biochem Biophys Res Commun 677, 155-161 (2023)
   PUBMED   37591184
  REMARK    GeneRIF: Structural basis of the human negative elongation factor
            NELF-B/C/E ternary complex.
REFERENCE   2  (bases 1 to 2540)
  AUTHORS   Awah,C.U., Winter,J., Mazdoom,C.M. and Ogunwobi,O.O.
  TITLE     NSUN6, an RNA methyltransferase of 5-mC controls glioblastoma
            response to temozolomide (TMZ) via NELFB and RPS6KB2 interaction
  JOURNAL   Cancer Biol Ther 22 (10-12), 587-597 (2021)
   PUBMED   34705606
  REMARK    GeneRIF: NSUN6, an RNA methyltransferase of 5-mC controls
            glioblastoma response to temozolomide (TMZ) via NELFB and RPS6KB2
            interaction.
REFERENCE   3  (bases 1 to 2540)
  AUTHORS   Huttlin,E.L., Bruckner,R.J., Navarrete-Perea,J., Cannon,J.R.,
            Baltier,K., Gebreab,F., Gygi,M.P., Thornock,A., Zarraga,G., Tam,S.,
            Szpyt,J., Gassaway,B.M., Panov,A., Parzen,H., Fu,S., Golbazi,A.,
            Maenpaa,E., Stricker,K., Guha Thakurta,S., Zhang,T., Rad,R.,
            Pan,J., Nusinow,D.P., Paulo,J.A., Schweppe,D.K., Vaites,L.P.,
            Harper,J.W. and Gygi,S.P.
  TITLE     Dual proteome-scale networks reveal cell-specific remodeling of the
            human interactome
  JOURNAL   Cell 184 (11), 3022-3040 (2021)
   PUBMED   33961781
REFERENCE   4  (bases 1 to 2540)
  AUTHORS   Luck,K., Kim,D.K., Lambourne,L., Spirohn,K., Begg,B.E., Bian,W.,
            Brignall,R., Cafarelli,T., Campos-Laborie,F.J., Charloteaux,B.,
            Choi,D., Cote,A.G., Daley,M., Deimling,S., Desbuleux,A., Dricot,A.,
            Gebbia,M., Hardy,M.F., Kishore,N., Knapp,J.J., Kovacs,I.A.,
            Lemmens,I., Mee,M.W., Mellor,J.C., Pollis,C., Pons,C.,
            Richardson,A.D., Schlabach,S., Teeking,B., Yadav,A., Babor,M.,
            Balcha,D., Basha,O., Bowman-Colin,C., Chin,S.F., Choi,S.G.,
            Colabella,C., Coppin,G., D'Amata,C., De Ridder,D., De Rouck,S.,
            Duran-Frigola,M., Ennajdaoui,H., Goebels,F., Goehring,L., Gopal,A.,
            Haddad,G., Hatchi,E., Helmy,M., Jacob,Y., Kassa,Y., Landini,S.,
            Li,R., van Lieshout,N., MacWilliams,A., Markey,D., Paulson,J.N.,
            Rangarajan,S., Rasla,J., Rayhan,A., Rolland,T., San-Miguel,A.,
            Shen,Y., Sheykhkarimli,D., Sheynkman,G.M., Simonovsky,E., Tasan,M.,
            Tejeda,A., Tropepe,V., Twizere,J.C., Wang,Y., Weatheritt,R.J.,
            Weile,J., Xia,Y., Yang,X., Yeger-Lotem,E., Zhong,Q., Aloy,P.,
            Bader,G.D., De Las Rivas,J., Gaudet,S., Hao,T., Rak,J.,
            Tavernier,J., Hill,D.E., Vidal,M., Roth,F.P. and Calderwood,M.A.
  TITLE     A reference map of the human binary protein interactome
  JOURNAL   Nature 580 (7803), 402-408 (2020)
   PUBMED   32296183
REFERENCE   5  (bases 1 to 2540)
  AUTHORS   Yun,H., Bedolla,R., Horning,A., Li,R., Chiang,H.C., Huang,T.H.,
            Reddick,R., Olumi,A.F., Ghosh,R. and Kumar,A.P.
  TITLE     BRCA1 Interacting Protein COBRA1 Facilitates Adaptation to
            Castrate-Resistant Growth Conditions
  JOURNAL   Int J Mol Sci 19 (7), 2104 (2018)
   PUBMED   30036938
  REMARK    GeneRIF: BRCA1 Interacting Protein COBRA1 Facilitates Adaptation to
            Castrate-Resistant Growth Conditions.
            Publication Status: Online-Only
REFERENCE   6  (bases 1 to 2540)
  AUTHORS   Pan,H., Zhao,X., Zhang,X., Abouelsoud,M., Sun,J., April,C.,
            Amleh,A., Fan,J.B., Hu,Y. and Li,R.
  TITLE     Translational Initiation at a Non-AUG Start Codon for Human and
            Mouse Negative Elongation Factor-B
  JOURNAL   PLoS One 10 (5), e0127422 (2015)
   PUBMED   26010750
  REMARK    GeneRIF: both human and mouse NELF-B proteins are translated from a
            non-AUG codon upstream of the annotated AUG.
            Publication Status: Online-Only
REFERENCE   7  (bases 1 to 2540)
  AUTHORS   Ye,Q., Hu,Y.F., Zhong,H., Nye,A.C., Belmont,A.S. and Li,R.
  TITLE     BRCA1-induced large-scale chromatin unfolding and allele-specific
            effects of cancer-predisposing mutations
  JOURNAL   J Cell Biol 155 (6), 911-921 (2001)
   PUBMED   11739404
REFERENCE   8  (bases 1 to 2540)
  AUTHORS   Ping,Y.H. and Rana,T.M.
  TITLE     DSIF and NELF interact with RNA polymerase II elongation complex
            and HIV-1 Tat stimulates P-TEFb-mediated phosphorylation of RNA
            polymerase II and DSIF during transcription elongation
  JOURNAL   J Biol Chem 276 (16), 12951-12958 (2001)
   PUBMED   11112772
REFERENCE   9  (bases 1 to 2540)
  AUTHORS   Simpson,J.C., Wellenreuther,R., Poustka,A., Pepperkok,R. and
            Wiemann,S.
  TITLE     Systematic subcellular localization of novel proteins identified by
            large-scale cDNA sequencing
  JOURNAL   EMBO Rep 1 (3), 287-292 (2000)
   PUBMED   11256614
REFERENCE   10 (bases 1 to 2540)
  AUTHORS   Yamaguchi,Y., Takagi,T., Wada,T., Yano,K., Furuya,A., Sugimoto,S.,
            Hasegawa,J. and Handa,H.
  TITLE     NELF, a multisubunit complex containing RD, cooperates with DSIF to
            repress RNA polymerase II elongation
  JOURNAL   Cell 97 (1), 41-51 (1999)
   PUBMED   10199401
COMMENT     VALIDATED REFSEQ: This record has undergone validation or
            preliminary review. The reference sequence was derived from
            DA210427.1, BC019029.1, AK022651.1 and AL050280.1.
            
            On Nov 23, 2018 this sequence version replaced NM_015456.4.
            
            Summary: NELFB is a subunit of negative elongation factor (NELF),
            which also includes NELFA (WHSC2; MIM 606026), either NELFC or
            NELFD (TH1L; MIM 605297), and NELFE (RDBP; MIM 154040). NELF acts
            with DRB sensitivity-inducing factor (DSIF), a heterodimer of SPT4
            (SUPT4H1; MIM 603555) and SPT5 (SUPT5H; MIM 602102), to cause
            transcriptional pausing of RNA polymerase II (see MIM 180660)
            (Narita et al., 2003 [PubMed 12612062]).[supplied by OMIM, Mar
            2008].
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: BC019029.1, AK091056.1 [ECO:0000332]
            RNAseq introns              :: single sample supports all introns
                                           SAMEA1965299, SAMEA1966682
                                           [ECO:0000348]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match       :: ENST00000343053.6/ ENSP00000339495.6
            non-AUG initiation codon :: PMID: 26010750
            RefSeq Select criteria   :: based on single protein-coding
                                        transcript
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-23                DA210427.1         21-43
            24-1733             BC019029.1         10-1719
            1734-2537           AK022651.1         1257-2060
            2538-2540           AL050280.1         1812-1814
FEATURES             Location/Qualifiers
     source          1..2540
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="9"
                     /map="9q34.3"
     gene            1..2540
                     /gene="NELFB"
                     /gene_synonym="COBRA1; NELF-B"
                     /note="negative elongation factor complex member B"
                     /db_xref="GeneID:25920"
                     /db_xref="HGNC:HGNC:24324"
                     /db_xref="MIM:611180"
     exon            1..285
                     /gene="NELFB"
                     /gene_synonym="COBRA1; NELF-B"
                     /inference="alignment:Splign:2.1.0"
     CDS             40..1926
                     /gene="NELFB"
                     /gene_synonym="COBRA1; NELF-B"
                     /note="non-AUG (CUG) translation initiation codon;
                     negative elongation factor protein B; cofactor of BRCA1"
                     /codon_start=1
                     /product="negative elongation factor B"
                     /protein_id="NP_056271.3"
                     /db_xref="CCDS:CCDS7040.2"
                     /db_xref="GeneID:25920"
                     /db_xref="HGNC:HGNC:24324"
                     /db_xref="MIM:611180"
                     /translation="MAELEGAGERGSGGPRGPAERASGVSAAAPGERAGDGAPSRAVA
                     GASAMFAGLQDLGVANGEDLKETLTNCTEPLKAIEQFQTENGVLLPSLQSALPFLDLH
                     GTPRLEFHQSVFDELRDKLLERVSAIASEGKAEERYKKLEDLLEKSFSLVKMPSLQPV
                     VMCVMKHLPKVPEKKLKLVMADKELYRACAVEVKRQIWQDNQALFGDEVSPLLKQYIL
                     EKESALFSTELSVLHNFFSPSPKTRRQGEVVQRLTRMVGKNVKLYDMVLQFLRTLFLR
                     TRNVHYCTLRAELLMSLHDLDVGEICTVDPCHKFTWCLDACIRERFVDSKRARELQGF
                     LDGVKKGQEQVLGDLSMILCDPFAINTLALSTVRHLQELVGQETLPRDSPDLLLLLRL
                     LALGQGAWDMIDSQVFKEPKMEVELITRFLPMLMSFLVDDYTFNVDQKLPAEEKAPVS
                     YPNTLPESFTKFLQEQRMACEVGLYYVLHITKQRNKNALLRLLPGLVETFGDLAFGDI
                     FLHLLTGNLALLADEFALEDFCSSLFDGFFLTASPRKENVHRHALRLLIHLHPRVAPS
                     KLEALQKALEPTGQSGEAVKELYSQLGEKLEQLDHRKPSPAQAAETPALELPLPSVPA
                     PAPL"
     exon            286..449
                     /gene="NELFB"
                     /gene_synonym="COBRA1; NELF-B"
                     /inference="alignment:Splign:2.1.0"
     exon            450..549
                     /gene="NELFB"
                     /gene_synonym="COBRA1; NELF-B"
                     /inference="alignment:Splign:2.1.0"
     exon            550..780
                     /gene="NELFB"
                     /gene_synonym="COBRA1; NELF-B"
                     /inference="alignment:Splign:2.1.0"
     exon            781..966
                     /gene="NELFB"
                     /gene_synonym="COBRA1; NELF-B"
                     /inference="alignment:Splign:2.1.0"
     exon            967..1079
                     /gene="NELFB"
                     /gene_synonym="COBRA1; NELF-B"
                     /inference="alignment:Splign:2.1.0"
     exon            1080..1182
                     /gene="NELFB"
                     /gene_synonym="COBRA1; NELF-B"
                     /inference="alignment:Splign:2.1.0"
     exon            1183..1278
                     /gene="NELFB"
                     /gene_synonym="COBRA1; NELF-B"
                     /inference="alignment:Splign:2.1.0"
     exon            1279..1421
                     /gene="NELFB"
                     /gene_synonym="COBRA1; NELF-B"
                     /inference="alignment:Splign:2.1.0"
     exon            1422..1528
                     /gene="NELFB"
                     /gene_synonym="COBRA1; NELF-B"
                     /inference="alignment:Splign:2.1.0"
     exon            1529..1670
                     /gene="NELFB"
                     /gene_synonym="COBRA1; NELF-B"
                     /inference="alignment:Splign:2.1.0"
     exon            1671..1779
                     /gene="NELFB"
                     /gene_synonym="COBRA1; NELF-B"
                     /inference="alignment:Splign:2.1.0"
     exon            1780..2540
                     /gene="NELFB"
                     /gene_synonym="COBRA1; NELF-B"
                     /inference="alignment:Splign:2.1.0"
     regulatory      2520..2525
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NELFB"
                     /gene_synonym="COBRA1; NELF-B"
                     /note="hexamer: AATAAA"
     polyA_site      2540
                     /gene="NELFB"
                     /gene_synonym="COBRA1; NELF-B"
                     /note="major polyA site"
ORIGIN      
        1 ggctgcggga cgcgcgcgga gtcgcgcggc gggcgggacc tggccgagct ggagggcgcc
       61 ggggagcggg gctcgggcgg tccccgaggc ccggcggagc gggcttctgg ggtgtctgcg
      121 gcggcgccgg gggaacgggc tggggatggg gcgcctagcc gggcggtggc cggggcctcg
      181 gccatgttcg cggggctgca ggacctgggc gtggccaacg gcgaggacct gaaggagacc
      241 ctgaccaact gcacggagcc gctcaaggcc atcgagcagt tccagacaga gaatggtgtg
      301 ctgctgccat ctcttcagtc agccctcccc ttcttggacc tgcacgggac gccgcggctg
      361 gagttccacc agtcggtatt cgatgagctg cgggacaagc tgctggagcg agtgtcagcc
      421 atcgcttcgg aggggaaggc tgaggaaagg tacaagaagc tggaagacct tctggagaag
      481 agcttttctc tggtgaagat gccgtccctg cagcccgtgg tgatgtgcgt catgaagcac
      541 ctgcccaagg ttccggagaa aaaactgaag ctggttatgg ctgacaagga gctgtatcga
      601 gcctgcgccg tggaggtgaa gcggcagatc tggcaagaca accaggccct cttcggggac
      661 gaggtttccc cactcctgaa gcagtacatc ctggagaagg agagcgctct cttcagtaca
      721 gagctctctg tcctgcacaa ctttttcagt ccttccccca agaccaggcg ccagggcgag
      781 gtggtgcagc ggctgacgcg gatggtgggg aagaacgtga agctgtacga catggtgctg
      841 cagtttctgc gcacgctctt cctgcgcacg cggaatgtgc actactgcac gctgcgggct
      901 gagctgctca tgtccctgca cgacctggac gtgggtgaaa tctgcaccgt ggacccgtgc
      961 cacaagttca cctggtgcct ggacgcctgc atccgagagc ggttcgtgga cagcaagagg
     1021 gcgcgggagc tgcaggggtt tctcgatggc gtcaagaagg gccaggagca ggtgctgggg
     1081 gacctgtcca tgatcctgtg tgaccccttc gccatcaaca cgctggcact gagcacagtc
     1141 aggcacctgc aggagctggt cggccaggag acactgccca gggacagccc cgacctcctg
     1201 ctgctgctcc ggctgctggc gctgggccag ggagcctggg acatgatcga cagccaggtc
     1261 ttcaaggagc ccaagatgga ggtagagctc atcaccaggt tcctcccgat gctcatgtcc
     1321 ttcctggtgg atgactacac tttcaatgtg gatcagaaac ttccggctga ggagaaagcc
     1381 ccagtctcat atccaaacac acttcccgaa agcttcacta agtttctgca ggagcagcgc
     1441 atggcctgcg aggtggggct gtactacgtc ctgcacatca ccaagcagag gaacaagaac
     1501 gcgctcctcc gcctgctgcc cgggctggtg gagacctttg gcgacttggc ctttggcgac
     1561 atcttcctcc acctgctcac gggcaacctt gcgctgctgg ccgacgaatt tgcccttgag
     1621 gacttctgca gcagcctctt cgatggcttc ttcctcaccg cctctccaag gaaggagaac
     1681 gtgcaccggc acgcgctgcg gctcctcatt cacctgcacc ccagggtggc cccgtctaag
     1741 ctggaggcgt tgcagaaggc cctggagcct acaggccaga gcggagaggc agtgaaggag
     1801 ctttactccc agctcggcga gaagctggaa cagctggatc accggaagcc cagcccggca
     1861 caggctgcgg agacgccggc cctggagctg cccctcccca gcgtgcccgc ccctgccccg
     1921 ctctgagggc cctccagacc tgctcgggtg ctggggccat gccgagtcgc ggccctgctc
     1981 agccggaaga ggctcccgga cctggatgta cagggcagtc tctcttcccg gggctatggc
     2041 tgggcctgtc ctgccgtcat ggccccctgc ttcctgctcc ttggagctgg ctcccggacc
     2101 ttgcccacca tccatgcagt ggctcccagg gcagagcctc tccttgtact ttggcagcca
     2161 tagaaagcgt gctcattttc tgttttcctg tgttaggaaa aaaccacctg ttttccaagg
     2221 ggagagggcg gggcctgagg gtgggggcgg ggcctcttca ttggcccagc ttggcgaaag
     2281 cgaggcacac tgcttactgc cttggggttg tggagatgga cccgtgacct cgtggaggcc
     2341 gtgtgggggc agcagcctgg cctgtgccat ggtgggtgtc ctggggcctg tgcggaggga
     2401 gccacctcac cctgcagccc agtttgcagg tgtggccttg tttctccttg cccagcagtg
     2461 ctgccttcag cggccgtgac ggggccagct ggacacacgg tgagattttc tcgtatgtaa
     2521 ataaaaggca ttttggtaaa
//
