LOCUS       NM_033116               8278 bp    mRNA    linear   PRI 07-FEB-2025
DEFINITION  Homo sapiens NIMA related kinase 9 (NEK9), transcript variant 2,
            mRNA.
ACCESSION   NM_033116
VERSION     NM_033116.6
KEYWORDS    RefSeq; MANE Select.
SOURCE      Homo sapiens (human)
  ORGANISM  Homo sapiens
            Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
            Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
            Catarrhini; Hominidae; Homo.
REFERENCE   1  (bases 1 to 8278)
  AUTHORS   Segal,D., Maier,S., Mastromarco,G.J., Qian,W.W., Nabeel-Shah,S.,
            Lee,H., Moore,G., Lacoste,J., Larsen,B., Lin,Z.Y.,
            Selvabaskaran,A., Liu,K., Smibert,C., Zhang,Z., Greenblatt,J.,
            Peng,J., Lee,H.O., Gingras,A.C. and Taipale,M.
  TITLE     A central chaperone-like role for 14-3-3 proteins in human cells
  JOURNAL   Mol Cell 83 (6), 974-993 (2023)
   PUBMED   36931259
REFERENCE   2  (bases 1 to 8278)
  AUTHORS   Muller,M., Eghbalian,R., Boeckel,J.N., Frese,K.S., Haas,J.,
            Kayvanpour,E., Sedaghat-Hamedani,F., Lackner,M.K., Tugrul,O.F.,
            Ruppert,T., Tappu,R., Martins Bordalo,D., Kneuer,J.M., Piekarek,A.,
            Herch,S., Schudy,S., Keller,A., Grammes,N., Bischof,C., Klinke,A.,
            Cardoso-Moreira,M., Kaessmann,H., Katus,H.A., Frey,N.,
            Steinmetz,L.M. and Meder,B.
  TITLE     NIMA-related kinase 9 regulates the phosphorylation of the
            essential myosin light chain in the heart
  JOURNAL   Nat Commun 13 (1), 6209 (2022)
   PUBMED   36266340
  REMARK    GeneRIF: NIMA-related kinase 9 regulates the phosphorylation of the
            essential myosin light chain in the heart.
            Publication Status: Online-Only
REFERENCE   3  (bases 1 to 8278)
  AUTHORS   Zhaoyu,Z., Huanyu,X., Yajie,Z., Ziyu,D., Hui,L. and Feng,S.
  TITLE     Study on the Expression of lncRNA ATB and Nek9 in Breast Cancer
            Patients Based on Q-PCR Technology and Its Relationship with the
            Disease
  JOURNAL   Contrast Media Mol Imaging 2022, 2634080 (2022)
   PUBMED   35935324
  REMARK    GeneRIF: Study on the Expression of lncRNA ATB and Nek9 in Breast
            Cancer Patients Based on Q-PCR Technology and Its Relationship with
            the Disease.
            Publication Status: Online-Only
REFERENCE   4  (bases 1 to 8278)
  AUTHORS   Cho,N.H., Cheveralls,K.C., Brunner,A.D., Kim,K., Michaelis,A.C.,
            Raghavan,P., Kobayashi,H., Savy,L., Li,J.Y., Canaj,H., Kim,J.Y.S.,
            Stewart,E.M., Gnann,C., McCarthy,F., Cabrera,J.P., Brunetti,R.M.,
            Chhun,B.B., Dingle,G., Hein,M.Y., Huang,B., Mehta,S.B.,
            Weissman,J.S., Gomez-Sjoberg,R., Itzhak,D.N., Royer,L.A., Mann,M.
            and Leonetti,M.D.
  TITLE     OpenCell: Endogenous tagging for the cartography of human cellular
            organization
  JOURNAL   Science 375 (6585), eabi6983 (2022)
   PUBMED   35271311
REFERENCE   5  (bases 1 to 8278)
  AUTHORS   Benz,C., Ali,M., Krystkowiak,I., Simonetti,L., Sayadi,A.,
            Mihalic,F., Kliche,J., Andersson,E., Jemth,P., Davey,N.E. and
            Ivarsson,Y.
  TITLE     Proteome-scale mapping of binding sites in the unstructured regions
            of the human proteome
  JOURNAL   Mol Syst Biol 18 (1), e10584 (2022)
   PUBMED   35044719
REFERENCE   6  (bases 1 to 8278)
  AUTHORS   Belham,C., Roig,J., Caldwell,J.A., Aoyama,Y., Kemp,B.E., Comb,M.
            and Avruch,J.
  TITLE     A mitotic cascade of NIMA family kinases. Nercc1/Nek9 activates the
            Nek6 and Nek7 kinases
  JOURNAL   J Biol Chem 278 (37), 34897-34909 (2003)
   PUBMED   12840024
  REMARK    GeneRIF: Activated in mitosis, and activates nek6 and nek7 kinase.
REFERENCE   7  (bases 1 to 8278)
  AUTHORS   Heilig,R., Eckenberg,R., Petit,J.L., Fonknechten,N., Da Silva,C.,
            Cattolico,L., Levy,M., Barbe,V., de Berardinis,V., Ureta-Vidal,A.,
            Pelletier,E., Vico,V., Anthouard,V., Rowen,L., Madan,A., Qin,S.,
            Sun,H., Du,H., Pepin,K., Artiguenave,F., Robert,C., Cruaud,C.,
            Bruls,T., Jaillon,O., Friedlander,L., Samson,G., Brottier,P.,
            Cure,S., Segurens,B., Aniere,F., Samain,S., Crespeau,H., Abbasi,N.,
            Aiach,N., Boscus,D., Dickhoff,R., Dors,M., Dubois,I., Friedman,C.,
            Gouyvenoux,M., James,R., Madan,A., Mairey-Estrada,B., Mangenot,S.,
            Martins,N., Menard,M., Oztas,S., Ratcliffe,A., Shaffer,T.,
            Trask,B., Vacherie,B., Bellemere,C., Belser,C., Besnard-Gonnet,M.,
            Bartol-Mavel,D., Boutard,M., Briez-Silla,S., Combette,S.,
            Dufosse-Laurent,V., Ferron,C., Lechaplais,C., Louesse,C.,
            Muselet,D., Magdelenat,G., Pateau,E., Petit,E.,
            Sirvain-Trukniewicz,P., Trybou,A., Vega-Czarny,N., Bataille,E.,
            Bluet,E., Bordelais,I., Dubois,M., Dumont,C., Guerin,T.,
            Haffray,S., Hammadi,R., Muanga,J., Pellouin,V., Robert,D.,
            Wunderle,E., Gauguet,G., Roy,A., Sainte-Marthe,L., Verdier,J.,
            Verdier-Discala,C., Hillier,L., Fulton,L., McPherson,J.,
            Matsuda,F., Wilson,R., Scarpelli,C., Gyapay,G., Wincker,P.,
            Saurin,W., Quetier,F., Waterston,R., Hood,L. and Weissenbach,J.
  TITLE     The DNA sequence and analysis of human chromosome 14
  JOURNAL   Nature 421 (6923), 601-607 (2003)
   PUBMED   12508121
  REMARK    Erratum:[Nature. 2023 Aug;620(7973):E17. doi:
            10.1038/s41586-023-06403-y.. PMID: 37491470]
REFERENCE   8  (bases 1 to 8278)
  AUTHORS   Roig,J., Mikhailov,A., Belham,C. and Avruch,J.
  TITLE     Nercc1, a mammalian NIMA-family kinase, binds the Ran GTPase and
            regulates mitotic progression
  JOURNAL   Genes Dev 16 (13), 1640-1658 (2002)
   PUBMED   12101123
  REMARK    GeneRIF: binds the Ran GTPase and regulates mitotic progression
REFERENCE   9  (bases 1 to 8278)
  AUTHORS   Holland,P.M., Milne,A., Garka,K., Johnson,R.S., Willis,C.,
            Sims,J.E., Rauch,C.T., Bird,T.A. and Virca,G.D.
  TITLE     Purification, cloning, and characterization of Nek8, a novel
            NIMA-related kinase, and its candidate substrate Bicd2
  JOURNAL   J Biol Chem 277 (18), 16229-16240 (2002)
   PUBMED   11864968
REFERENCE   10 (bases 1 to 8278)
  AUTHORS   Ohara,O., Nagase,T., Mitsui,G., Kohga,H., Kikuno,R., Hiraoka,S.,
            Takahashi,Y., Kitajima,S., Saga,Y. and Koseki,H.
  TITLE     Characterization of size-fractionated cDNA libraries generated by
            the in vitro recombination-assisted method
  JOURNAL   DNA Res 9 (2), 47-57 (2002)
   PUBMED   12056414
COMMENT     REVIEWED REFSEQ: This record has been curated by NCBI staff. The
            reference sequence was derived from AL049780.4.
            This sequence is a reference standard in the RefSeqGene project.
            
            On Nov 23, 2018 this sequence version replaced NM_033116.5.
            
            Summary: This gene encodes a member of the NimA (never in mitosis
            A) family of serine/threonine protein kinases. The encoded protein
            is activated in mitosis and, in turn, activates other family
            members during mitosis. This protein also mediates cellular
            processes that are essential for interphase progression. [provided
            by RefSeq, Jul 2016].
            
            Sequence Note: This RefSeq record was created from transcript and
            genomic sequence data to make the sequence consistent with the
            reference genome assembly. The genomic coordinates used for the
            transcript record were based on transcript alignments.
            
            Publication Note:  This RefSeq record includes a subset of the
            publications that are available for this gene. Please see the Gene
            record to access additional publications.
            
            ##Evidence-Data-START##
            Transcript exon combination :: AB082526.1, SRR1803615.156200.1
                                           [ECO:0000332]
            RNAseq introns              :: mixed sample support SAMEA1965299,
                                           SAMEA1966682 [ECO:0006172]
            ##Evidence-Data-END##
            
            ##RefSeq-Attributes-START##
            MANE Ensembl match     :: ENST00000238616.10/ ENSP00000238616.5
            RefSeq Select criteria :: based on conservation, expression
            ##RefSeq-Attributes-END##
            COMPLETENESS: full length.
PRIMARY     REFSEQ_SPAN         PRIMARY_IDENTIFIER PRIMARY_SPAN        COMP
            1-346               AL049780.4         34706-35051
            347-524             AL049780.4         37531-37708
            525-580             AL049780.4         40580-40635
            581-651             AL049780.4         41174-41244
            652-757             AL049780.4         42819-42924
            758-889             AL049780.4         44428-44559
            890-1000            AL049780.4         47441-47551
            1001-1065           AL049780.4         48351-48415
            1066-1116           AL049780.4         51383-51433
            1117-1309           AL049780.4         51877-52069
            1310-1454           AL049780.4         54267-54411
            1455-1655           AL049780.4         55052-55252
            1656-1702           AL049780.4         55758-55804
            1703-1858           AL049780.4         57757-57912
            1859-1967           AL049780.4         59989-60097
            1968-2129           AL049780.4         60601-60762
            2130-2300           AL049780.4         64484-64654
            2301-2360           AL049780.4         66323-66382
            2361-2569           AL049780.4         70276-70484
            2570-2731           AL049780.4         73113-73274
            2732-2944           AL049780.4         74524-74736
            2945-8278           AL049780.4         77068-82401
FEATURES             Location/Qualifiers
     source          1..8278
                     /organism="Homo sapiens"
                     /mol_type="mRNA"
                     /db_xref="taxon:9606"
                     /chromosome="14"
                     /map="14q24.3"
     gene            1..8278
                     /gene="NEK9"
                     /gene_synonym="APUG; LCCS10; NC; NERCC; NERCC1"
                     /note="NIMA related kinase 9"
                     /db_xref="GeneID:91754"
                     /db_xref="HGNC:HGNC:18591"
                     /db_xref="MIM:609798"
     exon            1..346
                     /gene="NEK9"
                     /gene_synonym="APUG; LCCS10; NC; NERCC; NERCC1"
                     /inference="alignment:Splign:2.1.0"
     CDS             128..3067
                     /gene="NEK9"
                     /gene_synonym="APUG; LCCS10; NC; NERCC; NERCC1"
                     /EC_number="2.7.11.1"
                     /note="isoform 2 is encoded by transcript variant 2;
                     serine/threonine-protein kinase Nek9; nercc1 kinase;
                     nimA-related protein kinase 9; NIMA (never in mitosis gene
                     a)- related kinase 9"
                     /codon_start=1
                     /product="serine/threonine-protein kinase Nek9 isoform 2"
                     /protein_id="NP_149107.4"
                     /db_xref="CCDS:CCDS9839.1"
                     /db_xref="GeneID:91754"
                     /db_xref="HGNC:HGNC:18591"
                     /db_xref="MIM:609798"
                     /translation="MSVLGEYERHCDSINSDFGSESGGCGDSSPGPSASQGPRAGGGA
                     AEQEELHYIPIRVLGRGAFGEATLYRRTEDDSLVVWKEVDLTRLSEKERRDALNEIVI
                     LALLQHDNIIAYYNHFMDNTTLLIELEYCNGGNLYDKILRQKDKLFEEEMVVWYLFQI
                     VSAVSCIHKAGILHRDIKTLNIFLTKANLIKLGDYGLAKKLNSEYSMAETLVGTPYYM
                     SPELCQGVKYNFKSDIWAVGCVIFELLTLKRTFDATNPLNLCVKIVQGIRAMEVDSSQ
                     YSLELIQMVHSCLDQDPEQRPTADELLDRPLLRKRRREMEEKVTLLNAPTKRPRSSTV
                     TEAPIAVVTSRTSEVYVWGGGKSTPQKLDVIKSGCSARQVCAGNTHFAVVTVEKELYT
                     WVNMQGGTKLHGQLGHGDKASYRQPKHVEKLQGKAIRQVSCGDDFTVCVTDEGQLYAF
                     GSDYYGCMGVDKVAGPEVLEPMQLNFFLSNPVEQVSCGDNHVVVLTRNKEVYSWGCGE
                     YGRLGLDSEEDYYTPQKVDVPKALIIVAVQCGCDGTFLLTQSGKVLACGLNEFNKLGL
                     NQCMSGIINHEAYHEVPYTTSFTLAKQLSFYKIRTIAPGKTHTAAIDERGRLLTFGCN
                     KCGQLGVGNYKKRLGINLLGGPLGGKQVIRVSCGDEFTIAATDDNHIFAWGNGGNGRL
                     AMTPTERPHGSDICTSWPRPIFGSLHHVPDLSCRGWHTILIVEKVLNSKTIRSNSSGL
                     SIGTVFQSSSPGGGGGGGGGEEEDSQQESETPDPSGGFRGTMEADRGMEGLISPTEAM
                     GNSNGASSSCPGWLRKELENAEFIPMPDSPSPLSAAFSESEKDTLPYEELQGLKVASE
                     APLEHKPQVEASSPRLNPAVTCAGKGTPLTPPACACSSLQVEVERLQGLVLKCLAEQQ
                     KLQQENLQIFTQLQKLNKKLEGGQQVGMHSKGTQTAKEEMEMDPKPDLDSDSWCLLGT
                     DSCRPSL"
     exon            347..524
                     /gene="NEK9"
                     /gene_synonym="APUG; LCCS10; NC; NERCC; NERCC1"
                     /inference="alignment:Splign:2.1.0"
     exon            525..580
                     /gene="NEK9"
                     /gene_synonym="APUG; LCCS10; NC; NERCC; NERCC1"
                     /inference="alignment:Splign:2.1.0"
     exon            581..651
                     /gene="NEK9"
                     /gene_synonym="APUG; LCCS10; NC; NERCC; NERCC1"
                     /inference="alignment:Splign:2.1.0"
     exon            652..757
                     /gene="NEK9"
                     /gene_synonym="APUG; LCCS10; NC; NERCC; NERCC1"
                     /inference="alignment:Splign:2.1.0"
     exon            758..889
                     /gene="NEK9"
                     /gene_synonym="APUG; LCCS10; NC; NERCC; NERCC1"
                     /inference="alignment:Splign:2.1.0"
     exon            890..1000
                     /gene="NEK9"
                     /gene_synonym="APUG; LCCS10; NC; NERCC; NERCC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1001..1065
                     /gene="NEK9"
                     /gene_synonym="APUG; LCCS10; NC; NERCC; NERCC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1066..1116
                     /gene="NEK9"
                     /gene_synonym="APUG; LCCS10; NC; NERCC; NERCC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1117..1309
                     /gene="NEK9"
                     /gene_synonym="APUG; LCCS10; NC; NERCC; NERCC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1310..1454
                     /gene="NEK9"
                     /gene_synonym="APUG; LCCS10; NC; NERCC; NERCC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1455..1655
                     /gene="NEK9"
                     /gene_synonym="APUG; LCCS10; NC; NERCC; NERCC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1656..1702
                     /gene="NEK9"
                     /gene_synonym="APUG; LCCS10; NC; NERCC; NERCC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1703..1858
                     /gene="NEK9"
                     /gene_synonym="APUG; LCCS10; NC; NERCC; NERCC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1859..1967
                     /gene="NEK9"
                     /gene_synonym="APUG; LCCS10; NC; NERCC; NERCC1"
                     /inference="alignment:Splign:2.1.0"
     exon            1968..2129
                     /gene="NEK9"
                     /gene_synonym="APUG; LCCS10; NC; NERCC; NERCC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2130..2300
                     /gene="NEK9"
                     /gene_synonym="APUG; LCCS10; NC; NERCC; NERCC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2301..2360
                     /gene="NEK9"
                     /gene_synonym="APUG; LCCS10; NC; NERCC; NERCC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2361..2569
                     /gene="NEK9"
                     /gene_synonym="APUG; LCCS10; NC; NERCC; NERCC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2570..2731
                     /gene="NEK9"
                     /gene_synonym="APUG; LCCS10; NC; NERCC; NERCC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2732..2944
                     /gene="NEK9"
                     /gene_synonym="APUG; LCCS10; NC; NERCC; NERCC1"
                     /inference="alignment:Splign:2.1.0"
     exon            2945..8278
                     /gene="NEK9"
                     /gene_synonym="APUG; LCCS10; NC; NERCC; NERCC1"
                     /inference="alignment:Splign:2.1.0"
     regulatory      5490..5495
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NEK9"
                     /gene_synonym="APUG; LCCS10; NC; NERCC; NERCC1"
                     /note="hexamer: AATAAA"
     polyA_site      5510
                     /gene="NEK9"
                     /gene_synonym="APUG; LCCS10; NC; NERCC; NERCC1"
                     /note="major polyA site"
     regulatory      6205..6210
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NEK9"
                     /gene_synonym="APUG; LCCS10; NC; NERCC; NERCC1"
                     /note="hexamer: AATAAA"
     polyA_site      6226
                     /gene="NEK9"
                     /gene_synonym="APUG; LCCS10; NC; NERCC; NERCC1"
     regulatory      8258..8263
                     /regulatory_class="polyA_signal_sequence"
                     /gene="NEK9"
                     /gene_synonym="APUG; LCCS10; NC; NERCC; NERCC1"
                     /note="hexamer: ATTAAA"
     polyA_site      8278
                     /gene="NEK9"
                     /gene_synonym="APUG; LCCS10; NC; NERCC; NERCC1"
ORIGIN      
        1 accatcgagc ttcgcggggt tgctgggctg acggatccgc gggccggcat ctgaagcgag
       61 cgggacgcag cgcggccagg gcctccgggc atacgcaggc tggtccccaa ggcccgcggc
      121 cgccgccatg tcggtgctgg gcgagtacga gcgacactgc gattccatca actcggactt
      181 tgggagcgag tccgggggtt gcggggactc gagtccgggg cctagcgcca gtcaggggcc
      241 gcgagccggc ggcggcgcgg cggagcagga ggaactgcac tacatcccca tccgcgtcct
      301 gggccgcggc gccttcgggg aagccacgct gtaccgccgc accgaggatg actcactggt
      361 tgtgtggaag gaagtcgatt tgacccggct gtctgagaag gaacgtcgtg atgccttgaa
      421 tgagattgtt attctggcac tgctgcagca cgacaacatt attgcctact acaatcactt
      481 catggacaat accacgctgc tgattgagct ggaatattgt aatggaggga acctgtatga
      541 caaaatcctt cgtcagaagg acaagttgtt tgaggaagag atggtggtgt ggtacctatt
      601 tcagattgtt tcagcagtga gctgcatcca taaagctgga atccttcata gagatataaa
      661 gacattaaat atttttctga ccaaggcaaa cctgataaaa cttggagatt atggcctagc
      721 aaagaaactt aattctgagt attccatggc tgagacgctt gtgggaaccc catattacat
      781 gtctccagag ctctgtcaag gagtaaagta caatttcaag tctgatatct gggcagttgg
      841 ctgcgtcatt tttgaactgc ttaccttaaa gaggacgttt gatgctacaa acccacttaa
      901 cctgtgtgtg aagatcgtgc aaggaattcg ggccatggaa gttgactcta gccagtactc
      961 tttggaattg atccaaatgg ttcattcgtg ccttgaccag gatcctgagc agagacctac
     1021 tgcagatgaa cttctagatc gccctcttct caggaaacgc aggagagaga tggaggaaaa
     1081 agtcactctg cttaatgcac ctacaaagag accaaggtca agcactgtga ctgaagcacc
     1141 cattgctgta gtaacatcac gaaccagtga agtctatgtt tggggtggtg gaaaatccac
     1201 cccccagaaa ctggatgtta tcaagagtgg ctgtagtgcc cggcaggtct gtgcagggaa
     1261 tacccacttt gctgtggtca cagtggagaa ggaactgtac acttgggtga acatgcaagg
     1321 aggcactaaa ctccatggtc agctgggcca tggagacaaa gcctcctatc gacagccaaa
     1381 gcatgtggaa aagttgcaag gcaaagctat ccgtcaggtg tcatgtggtg atgatttcac
     1441 tgtctgtgtg actgatgagg gtcagctcta tgccttcgga tcagattatt atggctgcat
     1501 gggggtggac aaagttgctg gccctgaagt gctagaaccc atgcagctga acttcttcct
     1561 cagcaatcca gtggagcagg tctcctgtgg agataatcat gtggtggttc tgacacgaaa
     1621 caaggaagtc tattcttggg gctgtggcga atatggacga ctgggtttgg attcagaaga
     1681 ggattattat acaccacaaa aggtggatgt tcccaaggcc ttgattattg ttgcagttca
     1741 atgtggctgt gatgggacat ttctgttgac ccagtcaggc aaagtgctgg cctgtggact
     1801 caatgaattc aataagctgg gtctgaatca gtgcatgtcg ggaattatca accatgaagc
     1861 ataccatgaa gttccctaca caacgtcctt taccttggcc aaacagttgt ccttttataa
     1921 gatccgtacc attgccccag gcaagactca cacagctgct attgatgagc gaggccggct
     1981 gctgaccttt ggctgcaaca agtgtgggca gctgggcgtt gggaactaca agaagcgtct
     2041 gggaatcaac ctgttggggg gaccccttgg tgggaagcaa gtgatcaggg tctcctgcgg
     2101 tgatgagttt accattgctg ccactgatga taatcacatt tttgcctggg gcaatggtgg
     2161 taatggccgc ctggcaatga cccccacaga gagaccacat ggctctgata tctgtacctc
     2221 atggcctcgg cctatttttg gatctctgca tcatgtcccg gacctgtctt gccgtggatg
     2281 gcataccatt ctcatcgttg agaaagtatt gaattctaag accatccgtt ccaatagcag
     2341 tggcttatcc attggaactg tgtttcagag ctctagcccg ggaggaggcg gcgggggcgg
     2401 cggtggtgaa gaagaggaca gtcagcagga atctgaaact cctgacccaa gtggaggctt
     2461 ccgaggaaca atggaagcag accgaggaat ggaaggttta atcagtccca cagaggccat
     2521 ggggaacagt aatggggcca gcagctcctg tcctggctgg cttcgaaagg agctggaaaa
     2581 tgcagaattt atccccatgc ctgacagccc atctcctctc agtgcagcgt tttcagaatc
     2641 tgagaaagat accctgccct atgaagagct gcaaggactc aaagtggcct ctgaagctcc
     2701 tttggaacac aaaccccaag tagaagcctc gtcacctcgg ctgaatcctg cagtaacctg
     2761 tgctgggaag ggaacaccac tgactcctcc tgcgtgtgcg tgcagctctc tgcaggtgga
     2821 ggttgagaga ttgcagggtc tggtgttaaa gtgtctggct gaacaacaga agctacagca
     2881 agaaaacctc cagattttta cccaactgca gaagttgaac aagaaattag aaggagggca
     2941 gcaggtgggg atgcattcca aaggaactca gacagcaaag gaagagatgg aaatggatcc
     3001 aaagcctgac ttagattcag attcctggtg cctcctggga acagactcct gtagacccag
     3061 cctctagtct cctgagcctg tagagccccc aggagactgg gacccaaaga acttcacagc
     3121 acacttaccg aatgcagaga gcagctttcc tggctttgtt cacttgcaga aaaggagcgc
     3181 aaggcagagg ctctgaagca ctttccttgt acatttggag agtggcattg ccttttagat
     3241 aggatctagg agtgatttta ttgttttgga gaatggaagg gcccccatgg ccctggcttt
     3301 gtcatcagtg actgccatag caacagcagc tctgtacctc atctgttgat cccacctttg
     3361 aagaggagac acagtgctca ccttaattgc gctggtagca gcttatatcc catgtatcat
     3421 tttcaccatt gattggaagc tgccttggga attcagtacc aggcattacc cctctgggtg
     3481 ggagagggag aagtgtaaag ttggagtggg ctggaatcag gtgtggcccg cccagtgtcc
     3541 tctgcagagt ggtgaagtag tctggccctc ttgggagccc tgagtccagg aaaatatgtc
     3601 tgatggagtc aatctagggc ttgtttcgaa aaagttcagt tactctgtgc agctaaatgc
     3661 tttaggagga aaggtaggct taggttgctt ttcctctgag ggttgattga aatttcttca
     3721 gtgaggaata gagaaagggc aggaccctcc tcatcacaca gctggtgttt caggctgtga
     3781 ccaatgcagg gtgggatttc ctaactgtgg atgaggggat gaggtgtctc tgagggatga
     3841 ggtgtctcag agaattgagt ccatggggca gtcagaatag ccttaagaga aaatcatgaa
     3901 ggagaagagg tcctccttta gctgcctcta cttggtatct tagagagggc ttagagggct
     3961 ctcagtcttc tgcccatgaa aagacttctt tgagcctctg ccttcatggc tcttagggtt
     4021 ctgatcttga tatcagcagc cccaaccact ttctttctga atgtctagtc agtatttttc
     4081 cccttttggt gttttatgaa gccatgtggt aacgaatgaa tctgtatcat ttttcctacc
     4141 tgagtggccc aaagccagca ccaaaccctg ggagtccctg aagcctaaca gaacaggtag
     4201 aacttgcaaa aggaatttgg ctgagagctc acttctaatc ctgtactcac tgtgtctttg
     4261 tagatagaaa caagctagct ttaccaaaga gaaatagtgt catagaagaa caaaacttca
     4321 tatagaaagt tctaggcaag tatttgatgg tttccttaag gatgtgagct ttgtatttcc
     4381 acctagcctt gtaaaatgtt cctgtggtat tttgtgtcac acatcctacc tttgatgagt
     4441 ctcacatccc accttcctat aaacagctaa attaattttg tttcatcttc cccagaccaa
     4501 aatgtttgat aatcttatca acattgtggg aggtcttgtg caatggaaat tttgccattt
     4561 ctccaaaact ggtggcataa aggctgatgc ttggggagaa ccccattgct cgggacaggc
     4621 aactctgttc aatgggatct tctttggttt gatgttccca ttgttttctc agttctggga
     4681 agcctagtac attagtacta atgtaatcac tgaaaccttt tcttgaaata agggaagcag
     4741 ccaaactttg attaaagttg caagttctgg ggacttgcgg gggttgtcat aaactgtaac
     4801 agtgggtttt ggttcagcat gtaaatgcaa ctttgatttt cttgaggacc gattgacctg
     4861 tcatgtccct gtatcctcat gctcatcatc tcagcaggcc tgagaggctg ggtcagtttg
     4921 ggtgttcatc atgaggattg cttctgccat ggagctgatg gacatgggca ggttgctgag
     4981 aaggtggggt gaaagtgagt gccgggggtg ggtgagtgcc ctggtcttgt tcatagggga
     5041 gcctttccct agcagtggaa cgctgtggtc attttctcta gcatattccc ttgggaagtc
     5101 tagatttgct attaatctgg ctgagaatct aagttctgtg ccttagagac agtttgcact
     5161 ttcccatatt gtgcctggga cagccatatg attttttttc ccaccaaaca agtatgcaaa
     5221 cagaaaccag ttcaaagggg gatggagtaa aagatgaggc agtagaaatg cctttgaatg
     5281 gttttctgta gctaattctc tttaaatttt gtcctgcttt ttttctttat gcagtgctag
     5341 gtgttttaag ttttctagta gtattgcttt tgagttacag tataacctga gttactcctc
     5401 tgctctaaca ttgttgcaga agaactagtt tattgttagc caggttgctt gaaaggttga
     5461 gagtggagtg gtttggcatt tctgttttaa ataaacattt aagctcttaa tcagtactgt
     5521 gcttcagtaa gcctgtgcat ggaccttagt ctctggggat aaaacatcca gattctaagc
     5581 ctccgtgaat ctttgattct gtgagtcttt atataaataa tgttcttgga acagaaacaa
     5641 tgcaagggac atctgcacag aaatattctt tgcttctctc cagtttctta ttcttgccag
     5701 cagaatcatt gctgctgcta cagctttttt cccaaaaatg tctaaaagtg ccgaaagtgc
     5761 agagggaggg aaaaaatgag ccactaaaag aaggatgatc ctttcttgta cctacctcct
     5821 cagtctcagt gggggccttt ccaagtgata atgccagttt atggtttgta aaacttttcc
     5881 ttcaaagaga ttgaactgaa tttggtctcc ctggcatagc acagccaagt tacaacttct
     5941 cttggctgag catttcactg aagtttaaag caaaagtaca ctaaaaaatt tcatttcact
     6001 tgccatatgt tcacaaacat tcataataga acaattcagg ggcaagaata cattttaaat
     6061 aaattcattc acatgaaatc cctaaacctc caaaaaagta ttcatgatgg tcagggcaga
     6121 gtgggctata atcaggacta taaaacatgt tcctaactgc tgcaaagcca aaaatcattt
     6181 attgtttcaa tgattaactg tggaaataaa cacgctgatc attaaacctc acagaatgat
     6241 gaacattagg gagaaaaact ccactatgtc acatctctgt taccacctgt gtagctgtgg
     6301 agagtgataa attgataatg aaagcaaaca ttggccagaa ggtgctgtgc tgcattacat
     6361 acattgctgt taagaaatac tgtctcgggc cagacgcagt ggctcacgcc tgtaatccca
     6421 gcactttggg aggccgaggc gggcggatca cgaggtcagg agatcgagac catcctggct
     6481 aacacagtga aatcccgtct ctactaaaaa tacaaaaaaa ttagctgggt gttgtggcag
     6541 gtgcctgtag tcccagctac tctggaggct gaggcaggag aatggcgtga acccaggagg
     6601 cggagtttgc agtgagctga gattgcgcca cggtactcca gcctgggcga cagagtgaga
     6661 ctccatctca aaaataaaaa aaaaaatact gtcttggcag gacaggcgcg gtggctcacg
     6721 cctgtaatcc cagcactttg ggaggccgag gtgggcaggt caggagttca agaccatcct
     6781 ggctaacaca gtgaaacccc atctctacta aaaatacaaa aaatcagccg ggcgtggtgg
     6841 tgggcgcctg tagtcccagc tactcgggag gctggggcag aagaatggcg tgaacccggg
     6901 aggcggagct tgcagtgggc caagatcact ccactgcact ccagcctggg tgacaagagc
     6961 gagactccgt ctcaaaaaaa aaaaaaaaga aatactgtct cggcacatct gcatacattt
     7021 ttaatgaaat gagtttccac atgaggagtt tccccgtgaa atttctccta tggtgtaacc
     7081 ttatgaactt tataatgttt acttgcacta aacatttctt ggataaagtt gtataaaata
     7141 cagacattaa gaagcagcat gcatttactg tcatcgtctg agctatccag gaagaaattc
     7201 agattttttt aaaaaaaggt tcttatctct tcccagagac cagatcaaga taaatttaaa
     7261 agatgaaaga tatttctttt ttttttttct tttttttttt ttgagatgga ttcttactag
     7321 gtcaccaggc tggagtgcag tggcgcgatc tcagctcact gcaacctctg cctcctgggt
     7381 tcaagcaatt ctcctgcctc agcctcccga gtagttggga ctacaggtgt gcgccaccac
     7441 gcccagctaa tttttgtatt tttagtagag acggggtttc accatgttgg ccaggatggt
     7501 ctcgatctct tgactttgtg atccgcccac ctcggcctcc caaagtgctg ggattacagg
     7561 catgagccac catgctcggc cttgaaagat atttctaata taaccaaggc ctaaacatgt
     7621 agagattaaa gggaacactt aaggcattga ctattgtttt atttctttag caaaatgaga
     7681 gcaaacaact gcttcctttt tagaaagcac tccccacata taacaacaat ttgttctctc
     7741 tggagggccc ggatctatga ccttactaac atccagcgat cccaaatcca agtgcctgtg
     7801 gtaccaggca gataaggtaa agaagtgaag caggccaagt gagactgcag tggtctggtg
     7861 ctaccattcc catctggagg agcatctgat tctaaacccc acccaatttg tgccatgcgg
     7921 gttgctggcc cagtgtttcc agaacttcca gtttttcaag agatgttcca tatcccagtt
     7981 tttaaatgtc agatcatatt atgaatgcta ggccagcgaa ataaaacatc tgtggagcaa
     8041 agcaatccac atgttgccat tttgtgacct tggccacagc ttactcttgc catctattgc
     8101 aatgtactgg tttacattcc cacttaaagt gtacatttca ttgaacttgc ctagggaatg
     8161 tacctattag gttaaatatc cattatctct gtgtgaggtt tccttaaggg gctaaccata
     8221 aagctttatt atgtgatacc tcctttcctt tggaacaatt aaaaagagtg caaagaaa
//
